2021 Extraction of Deoxyribonucleic Acid from fruits By: Zodyann Reid University College of the Cayman Islands Biology 101 December 9, 2021 Abstract Deoxyribonucleic acid is found in everything here on Earth but each one is different. This lab report will examine 3 different fruits to see the amount of DNA that is extracted for each. To determine what if type of fruit will affect the extraction process. In this lab we will look at the different DNA for each fruit and compare looks of each. Hypothesis The type of fruit affects the amount of DNA that is extracted. Method and Material 1. A kiwi 2. Half of a banana 3. ½ cup of blueberries. 4. 300ml of water 5. 6 tsp Dish washing liquid 6. 1 tbsp salt 7. Cold isotopy alcohol 8. Toothpicks 9. Three tall clear glass cups 10. Three small clear glass cups 11. Tea cloth/paper towel 12. Three clear zip lock bags 13. Glass measuring cup 14. Microscope 15. Glass Slides Method: 1. ½ cup of isotopy alcohol (rubbing alcohol) was placed in the freezer to cool. 2. Three fruits were prepared and were each in a zip lock bag. 3. The fruits were then mashed until the chunks had disappeared. 4. In a large glass measuring cup 6 tsp of dish wishing liquid, 300ml of water and 1 tbsp of salt were mixed together until well combined. 5. Tea cloth was folded into a funnel shape and placed into each tall clear glass. 6. Each fruit mixtures were poured into each of the glasses through the filters. 7. Each filtrate was then poured into a small clear glass; the cold isotopy alcohol was gently pouring on the side of each of cup to let it rest on the surface. 8. After poring the alcohol, let it sit for a few minutes until the top layer of alcohol if cloudy. 9. With your toothpick swirl it gently until there are stringy looking substance on the toothpick and then placed that substance on a glass slides. Result Visibility Kiwi Slightly Banana Yes Blueberry Yes Amount of DNA Thickness A lot Very watery A lot Slightly watery Little to none Very thickness Pictures of DNA under a Microscope The kiwi’s DNA is mainly straight and ridged looking The banana’s DNA was more cluster and curly The blueberry’s DNA was longer and further spaced out Conclusion My hypothesis was that the type of fruit will affect the amount of DNA that can be extracted and my results support my hypothesis. The results showed that the fruits with more flesh ( banana and kiwi) the DNA was more (while inside the cup) and the fruit with less flesh (blueberry) the DNA was way less. Throughout the experiment I learned the type of fruit also affects the visibility and the thickness of the DNA. The banana had a lot of DNA but was the hardness to extract while the kiwi was the hard one to see and also extract. The experiment went really well but one problem was the measurement for the alcohol was wrong so in turn I had to measure more alcohol and wait for it to cool to continue the experiment. The experiment does not really need improvement apart for getting the exact measurement for each material. Works Cited College, P. (2021, September 30). Lab report writing . Retrieved from Phoenix College libguides: https://phoenixcollege.libguides.com/c.php?g=225738&p=1496045 Hamilton, A. (2009). Perception of Different. n/a: Alexander Hamilton . science, M. (2015-2021). 5 biology experiments you can do at home. Retrieved from MEL Science : https://melscience.com/US-en/articles/5-biology-experiments-you-can-dohome/ https://www.biologycorner.com/worksheets/labreport.html https://www.youtube.com/watch?v=SQnOV47MwD0
