PRINCES TOWN WEST SECONDARY CAPE UNIT 2 CHEMISTRY LABS 2011 - 2012 Princes Town West Secondary Unit 2 Chemistry Labs 1|Page # Title Lab # 1 Organic Compounds : To determine which organic compound is contained in the bottles labelled A, B, C and D Organic Compounds: To determine which organic compound is contained in the bottles labelled P, Q, R and S. Plan and Design : Organic Chemistry Organic Chemistry: To investigate the relative acidities of alcohols, phenols and carboxylic acids Analytical Chemistry : The degree of uncertainty Crystallization Analytical Chemistry : Gravimetric Analysis - percentage oxalate in an unknown sample Analytical Chemistry : Gravimetric Analysis - Vitamin C Tablet Analytical Chemistry : Spectroscopy : UV/Vis Analytical Chemistry : Quantitative Titration Chromatography : Paper Chromatography Pigment in leaves Chromatography : Paper Chromatography Inks Solvent Extraction Plan Design: Environmental Chemistry Simple and Fractional Distillation Environmental Chemistry : Organic Compounds Plan Design: To determine the acidity / pH of a soil sample Analytical Chemistry Back Titration Environmental test for pollutants in water. Plan and Design : Analytical Chemistry (Gravimetric) Lab # 2 Lab # 3 Lab # 4 Lab # 5 Lab # 6 Lab # 7 Lab # 8 Lab# 9 Lab# 10 Lab# 11 Lab# 12 Lab# 13 Lab # 14 Lab # 15 Lab # 16 Lab # 17 Lab # 18 Lab # 19 Lab # 20 Princes Town West Secondary Unit 2 Chemistry Labs Skills 2|Page Lab # 1 Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Organic Compounds Aim : To determine which organic compound is contained in the bottles labelled A, B, C and D. Apparatus and Material : 1. Test Tubes 2. Boiling Tubes 3. 250 ml Beaker(Labelled Organic Waste) 4. Magnesium Powder or Turnings 5. Sodium Carbonate 6. 2,4dinitrophenyihydrazine 7. Aqueous Silver Nitrate 8. Aqueous Sodium Hydroxide 9. Aqueous Ammonia 10. Acidified Potassium Dichromate Aqueous solutions, A, B, C and D. Each solution contains one of the following. I. an alcohol II. an aldehyde III. a carboxylic acid IV. a ketone Procedure : 1. You are to perform the tests below and from the results establish which type of organic compound is contained in each of A, B, C and D. 2. After each test discard the contents of the tubes into the 250 cm3 beaker, labelled organic waste. Rinse and re-use the tubes for the remaining tests. 3. Tests for gases are at the end of this lab sheet. Princes Town West Secondary Unit 2 Chemistry Labs 3|Page Test (a) Place 1 cm depth of each of the solutions A, B, C and D into separate test-tubes. To each tube add a small quantity of magnesium powder or turnings. Identify any gas given off and record the test you used to make the identification. Test (b) Place 1 cm depth of each of the solutions A, B, C and D into separate test-tubes. To each tube add a small quantity of powdered sodium carbonate. Identify any gas given off and record the test you used to make the identification. Test (c) Place 1 cm depth of each of the solutions A, B, C and D into separate test-tubes. To each tube add 1 cm depth of 2,4-dinitrophenylhydrazine reagent. : Place 1 cm depth of each of the solutions A, B, C and D into separate test-tubes. Place 2 cm depth of aqueous silver nitrate in a boiling-tube and add to it 1 cm depth of aqueous sodium hydroxide. This will produce a precipitate of silver oxide. Test (d) Test (e) Observati ons and Results : Use a dropping pipette to add dilute aqueous ammonia to this mixture until the precipitate of silver oxide just dissolves. Do not add an excess of aqueous ammonia. To each of the tubes containing A, B, C and D add 1 cm depth of the silvercontaining solution you have just prepared. Place 1 cm depth of each of the solutions A, B, C and D into separate boiling-tubes. To each tube add a few drops of acidified potassium dichromate(V1) to give a yellow-orange solution. Warm the tube gently. Aqueous Solutions B C (Alcohol) (Ketone ) (Alde hyde) Test (a) No Rxn No Rxn No Rxn Test (b) No Rxn No Rxn No Rxn Test (c) No Visible Rxn Yellow brown/ (orange) ppt formed A No ppt formed ∴ Princes Town West Secondary Unit 2 Chemistry Labs Yello w brown / (orang e) ppt D (Carboxylic Acid) Pop with glowing splint ∴ H2 gas given off. Hence Compound D is the acid. effervescence and gas bubbled through Ca(OH)2 White ppt formed. Gas given off CO2. D is the carboxylic Acid. No Visible Rxn No ppt formed 4|Page Aldehyd e or Ketone No Visible Rxn Test (d) No ppt formed Test (e) Discussion : • Green solution formed indicating Cr3+ ions hence an alcohol or aldehyde No Visible Rxn No ppt formed No Visible Rxn forme d∴ Aldeh yde or Keton e Silver ppt forme d on ring of test tube ∴ silver mirror effect hence comp ound C is the Aldeh yde Green soluti on forme d indica ting Cr3+ ions hence an alcoh ol or aldeh yde No Visible Rxn No ppt formed No Visible Rxn Test (a) Compound D is the acid as H2 gas was given off and identified ○ Princes Town West Secondary Unit 2 Chemistry Labs 5|Page ○ • Test (b) Effervescence CO2 given off as confirmation with white ppt ○ forming in CaA(OH)2 2R-COOH(aq)+Na2CO3(s)yields 2R-COO-Na+(aq)+ ○ CO2g+H2O(l) CO2g+Ca(OH)2 yields CaCO3(s)+H2O(l) ○ ○ • ∴ Substance D is the acid as no other reactions were observed by the other substances. Test (c) Yellow brown (or orange) ppt seen for B and C indication it’s a ○ carbonyl compound. ○ ○ ○ • by the pop with the glowing splint. 2R-COOH(aq)+2Mg(s)yields 2R-COO-Mg+(aq)+ H2(g) ∴ Substance B and C are either a ketone or aldehyde RCHO+ C6H3NO22.NHNH2yields RCH=NNC6H3NO22 s+H2Ol RR'CO+ C6H3NO22.NHNH2yields R'RC=NNC6H3NO22 s+H2Ol Test (d) 2Ag(NH3)2 (aq)++ RCHO(l)+3OH(aq)- yields 2Ag(s)+ ○ RCOO(aq)-+ 4NH3 g+ 2H2Ol Tollen’s reagent only reacts with aldehydes to give the silver ○ mirror effect ∴ substance C is the aldehyde. • Test (e) Substance A is an Alcohol because it reduces aqueous K2Cr2O7 ○ to a green Cr3+ but did not react with either Tollen’s reagent or 2,4,-dinitrophenylhydrazine reagents. Substance B ∴ has to be the ketone by reason of ○ elimination and its reaction with 2,4 ,dinitrophenylhydrazine. Conclusio n : Princes Town West Secondary Unit 2 Chemistry Labs 6|Page gas test and test result ammonia, NH3 turns damp red litmus paper blue carbon dioxide, CO 2 gives a white ppt. with limewater (ppt. dissolves with excess CO 2) chlorine, 012 bleaches damp litmus paper hydrogen, H2 'pops' with a lighted splint oxygen, 02 sulphur dioxide, 80 2 relights a glowing splint turns potassium dichromate(VI) (aq) from orange to green Princes Town West Secondary Unit 2 Chemistry Labs 7|Page Lab # 2 Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Organic Compounds Aim : To determine which organic compound is contained in the bottles labelled P, Q, R and S. Apparatus and Material : 1. Test Tubes 2. Boiling Tubes 3. 250 ml Beaker(Labelled Organic Waste) 4. Magnesium Powder or Turnings 5. Sodium Carbonate 6. 2,4dinitrophenyihydrazine 7. Aqueous Silver Nitrate 8. Aqueous Sodium Hydroxide 9. Aqueous Ammonia 10. Acidified Potassium Dichromate You are provided with four organic substances P, Q, R and S. Each belongs to one of the following classes of compounds: I. An aromatic alcohol II. An alkene III. An alkane IV. An alkyl halide Procedure : 1. You are to perform the tests below and from the results establish which type of organic compound is contained in each of P, Q, R and S. 2. After each test discard the contents of the tubes into the 250 cm3 beaker, labelled organic waste. Rinse and re-use the tubes for the remaining tests. 3. Gloves, eye protection and lab coats must be worn. The Fume hood must be used where specified. Princes Town West Secondary Unit 2 Chemistry Labs 8|Page Place 5 drops of each of the substances P, Q, R and S into separate Test (a) Test (b) test-tubes. Cover each test-tube completely with foil. In the fume cu board add 5 dro s of bromine to each. Sto er for 5-10 minutes Place 5 drops of each of the substances P, Q, R and S into separate test-tubes. In the fume cupboard add 5 drops of bromine to each. Place 5 drops of each of the substances P, Q, R and S into separate Test (c) test-tubes. Add 2% aqueous potassium manganate(VII) drop wise. : Place 2 drops of each of the substances P, Q, R and S into separate Test (d) test-tubes. To each tube add 1 cm depth of aqueous Iron(III) chlorid solution. Warm if necessary. Test (e) Observatio ns and Results Place 2 drops of each of the substances P, Q, R and S into separate test-tubes. To each tube add 1 cm depth of aqueous silver nitrate Aqueous Solutions P Q R S Test (a) Test (b) : Test (c) Test (d) Test (e) Discussion : Princes Town West Secondary Unit 2 Chemistry Labs 9|Page Conclusion : Princes Town West Secondary Unit 2 Chemistry Labs 10 | P a g e Lab # 3 Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Plan and Design : Organic Chemistry Aim : Case You are provided with solutions VF 3, VF 4 and VF 5. The solutions are: 1.0 mol dm–3 Propanone 1.0 mol dm–3 Propanal 0.5 mol dm–3 Propanoic Acid You are to plan experiments that will enable you to identify the solution that matches each of : VF 3, VF 4 and VF 5. You have available the following apparatus: –10 °C to 110 °C thermometer, 100 cm3 beaker, measuring cylinder, and the following reagents LiAlH4, EDTA, Fehlings Reagent, 2,4 DNPH, and one of your choice. (a) You are to identify, by the minimum number of practical steps, which of the solutions contains Propanone Princes Town West Secondary Unit 2 Chemistry Labs 11 | P a g e 1. Apparatus and Material : Procedure : 2. 1. 2. 3. 4. 5. 6. Observatio ns and Results : Data Analysis and : Interpretati on Discussion : • • Conclusion : Lab # 4 Date: Skills Assessed: O/R/ R M/M A/I Princes Town West Secondary Unit 2 Chemistry Labs 12 | P a g e P/D Title : Organic Chemistry Aim : To investigate the relative acidities of alcohols, phenols and carboxylic acids The relative acidities of alcohols, phenols and carboxylic acids depends on the ease at which the molecule gives up a proton to water in an aqueous solution. Strong acids are those which easily donate a proton and hence is fully ionised in an aqueous solution. Example: HCl H+ + Cl- Theory Alcohols do not give up H+ ions readily due to the presence of the OH group, because the alkyl group donates electrons to the O atom, thereby decreasing the tendency for the H atom to ionise. ROH + H2O RO- + H3O+ Phenols tend to give up H+ more readily than alcohols because electrons : from the OH bond tends to be localized around the benzene ring, and thus increases the tendency for the H atom to ionise. Carboxylic acids are the strongest acid of all the three, and tends to give up H+ more readily than alcohol and phenols. The carboxyl group tends to pull electrons away from the OH bond, hence weakening the OH bond and making it easier for the H atom to ionise. RCOOH + Apparatus and Material : H2O RCOO- + H3O+ 1. Samples of Ethanol, Phenol and Ethanoic acid. 2. Magnesium Ribbon 3. Solid Na2CO3 Princes Town West Secondary Unit 2 Chemistry Labs 5. Apparatus to test gas, splint, litmus, Ca(OH)2(aq) 6. pH meter / pH paper 7. spatula 13 | P a g e 4. Test tubes Procedure : 8. Glass rod 1. A piece of Magnesium Ribbon was added to 4 cm3 of ethanol in a test tube and observations were recorded 2. Solid Na2CO3 was added to 4 cm3 of ethanol in a test tube and observations were recorded 3. The pH meter was then used to find the pH of ethanol, and this value was also recorded. 4. The above procedure was then repeated using phenol and ethanoic acid. Table 1: Observation s and Results Observation table Ethanol : Phenol Ethanoic Acid Magnesium Ribbon Solid Na2CO3 pH Data Analysis and Interpretati on 1. Which samples reacted with Mg? Write a balanced equation for any reaction which occurred. : 2. Which samples reaction with Solid Na2CO3? Write a balanced equation for any reaction that occurred. 3. Use the results of the experiment to deduce the order of increasing acidities of the samples. Discussion : Conclusion : • Princes Town West Secondary Unit 2 Chemistry Labs 14 | P a g e Princes Town West Secondary Unit 2 Chemistry Labs 15 | P a g e Princes Town West Secondary Unit 2 Chemistry Labs 16 | P a g e Princes Town West Secondary Unit 2 Chemistry Labs 17 | P a g e Princes Town West Secondary Unit 2 Chemistry Labs 18 | P a g e Princes Town West Secondary Unit 2 Chemistry Labs 19 | P a g e Lab # 5 Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Analytical Chemistry : The degree of uncertainty Aim : To determine the degree of uncertainty between a measuring cylinder and a burette. Apparatus and Material : 1. 50 cm3 burette (Class A) 2. 10 cm3 measuring cylinder (Class A) 3. Distilled water Procedure : 1. The burette was filled to a known volume and record the volume 2. 25 drops of water was delivered drop wise into a measuring cylinder and record both the measuring cylinder and new burette reading 3. This process was repeated in steps of 25 drops until 200 drops were delivered. 4. The results were tabulated. 5. A graph was drawn vol of burette against vol in cylinder 6. A best fit line was drawn 7. Calculate the Standard Deviation between volumes in the burette and the measuring cylinder. Observatio ns and Results : Table: 1 Readings obtained from the burette and measuring cylinder respectively. No. of Drops 25 50 75 100 125 150 200 Final Burette Reading / cm 3 Initial Burette Reading / cm 3 Total Vol of drops Burette / cm3 Vol in measuring cylinder / cm 3 Princes Town West Secondary Unit 2 Chemistry Labs 20 | P a g e S.D. between Readings Draw a graph of volume of burette versus volume of measuring cylinder Graph Data Analysis : 1. From the graph determine when 4 cm3 of the burette is delivered the volume of measuring cylinder? 2. From the graph when 8.2 cm3 of water is delivered from the burette the reading in the measuring cylinder ? 3. Which instrument is considered to be accurate 4. Which instrument is considered to be precise 5. Explain your answer for questions 3 and 4. Discussion : • • • • Conclusion : Lab # NA Date: Skills Assessed: O/R/R M/M A/I P/D Princes Town West Secondary Unit 2 Chemistry Labs 21 | P a g e Title : Analytical Chemistry : Measurements Aim : Uncertainty of measurements of volumes and masses Apparatu s and Material : Procedur e : Part A 1. Burette, 50 mL, Class B 2. Graduated pipette, 10 mL, Class B 3. Volumetric flasks, 25 mL, Class A and B 4. Measuring cylinder, 10 mL 5. 15 Vials and stoppers 6. Tissue 7. Top loading balance 8. Analytical balance 9. Unknown liquid, 200 mL. (0.789 g ml-1) 10. Distilled water 11. Pipette filler 1. All glassware was rinsed with distilled water, and then 2 mL of unknown liquid provided. 2. The empty vials and the volumetric flasks were labelled 3. The mass of the empty dried sample vials with their stoppers in place were weighed. Precaution was taken no to interchange stoppers among vials. 4. The burette, graduated pipette, and measuring cylinder were used to transfer 10 mL of the unknown liquid into different vials. 5. The vials were stoppered and their loaded weights were recorded. 6. This was repeated until a total of three (3) loaded sample vials for each piece of measuring equipment. 7. Results were recorded in Table 1. Part B 1. Each volumetric flask was filled to the 25 mL mark with the unknown liquid ensuring that no liquid is spilled on the outside. 2. The volumetric flasks were stoppered and there loaded weights were recorded. 3. The unknown liquid in the flasks were completely transferred into a Princes Town West Secondary Unit 2 Chemistry Labs 22 | P a g e sample vial, again ensuring that no liquid is spilled on the outside. 4. The empty volumetric flasks with their stoppers were reweighed. 5. Results were recorder in Table 1. 1. Using a top loading balance combined masses of 2, 4, 6, 8, and 10, 100 g weights were obtained. Part C 2. A graph was plotted of mass versus number of weights to obtain a best fit line through the points. 1. The weight of two 100 g weights were measured using a top loading balance first by: a. The individual masses and b. The combined masses of two 2. This procedure was then repeated on an analytical balance. Part D Observatio ns and Results : Part A and B: Table 1: Uncertainty of Measurements of Volumetric Apparatus Apparatus Burette 1 2 3 Loaded 19.11 75 19.0 251 18. 92 38 Empty 11.08 93 11.0 073 10. 89 85 Mass 8.028 2 8.01 78 8.0 25 3 Loaded 18.88 71 18.9 043 19. 06 29 Empty 10.89 47 10.8 963 11. 02 64 Mass 7.992 4 8.00 8 8.0 36 Class B ± 0.06 Graduated Pipette Class B ± 0.06 Princes Town West Secondary Unit 2 Chemistry Labs Average 8.0238 8.0123 23 | P a g e 5 Measuring Cylinder Loaded 18.79 94 18.7 795 18. 92 84 Empty 11.07 05 11.0 494 11. 21 51 Mass 7.728 9 7.73 01 7.7 13 3 Loaded 21.92 82 21.9 17 21. 91 5 Empty 14.02 95 14.0 325 14. 02 96 Mass 7.898 7 7.88 45 7.8 85 4 Loaded 23.66 78 23.6 576 23. 64 85 Empty 15.73 82 15.7 443 15. 75 62 Mass 7.929 6 7.91 33 7.8 92 3 Class B ± 0.1 @ 20°C Volumetric Flask A Class B ± 0.02 @ 20°C Volumetric Flask B Class B ± 0.04 @ 20°C 7.7241 7.8895 7.9117 Part C i. 100 g Weights Slope Mass (g) 2 200.04 4 400.07 6 600.1 8 800.14 10 1000.17 100.01625 Princes Town West Secondary Unit 2 Chemistry Labs 24 | P a g e ii. Mass (g) 10100 g Weights Group 1 Mass( g) Average 200. 040 400 .07 60 0.1 0 80 0. 14 10 00 .1 7 100.016 Group 2 200. 065 400 .17 60 0.2 4 80 0. 14 10 00 .3 6 100.037 Group 3 200. 03 400 .17 60 0.1 2 80 0. 11 10 00 .2 100.021 Group 4 200. 06 400 .12 60 0.1 7 80 0. 22 10 00 .0 7 100.001 Avera ge Masse s 100.019 Part D iii. Mass 1-100 g Weight Top Loading Balance Analytical Balance A 100.02 100.003 B 100.02 100.001 100.02 100.002 200.04 200.004 Average Mass Mass Both 100 g Weights Princes Town West Secondary Unit 2 Chemistry Labs 25 | P a g e Part C Graph Standard Deviation and Gossett T-Function of Part A and B Data Analysis Standard Deviation = S . D . 1 Apparatus Loaded : Empty Mass Loaded Empty Graduated Pipette Mass (xx)2 Measuring Cylinder Loaded Empty 3 19.117 5 11.089 3 19.02 51 11.00 73 18.923 8 10.898 5 8.0282 8.017 8 8.0253 Burette (xx)2 2 0.0000 197 0.000 0356 0.0000 02351 18.887 1 10.894 7 18.90 43 10.89 63 19.062 9 11.026 4 7.9924 8.008 8.0365 0.0003 96 0.000 018 0.0005 86 18.799 4 11.070 5 18.77 95 11.04 94 18.928 4 11.215 1 Princes Town West Secondary Unit 2 Chemistry Labs Av era ge (x x) 2 ( x x ) 2 2 Gossett’s T-Function t= 4.302 λ=sd×t(p,n-1)n 8.0 23 8 5. 76 1E 05 0 . 0 0 5 4 0.0133 1.0 00 E03 0. 0 2 2 4 0.0556 8.0 12 3 26 | P a g e Mass (xx)2 Loaded Empty Volumetric Flask A Mass (xx)2 Loaded Volumetric Flask B Empty Mass (xx)2 7.7289 7.730 1 0.0000 23 0.000 036 21.928 2 14.029 5 21.91 7 14.03 25 7.8987 7.884 5 7.7133 0.0001 17 0 . 0 0 9 4 0.0233 1. 26 4E 04 0 . 0 0 8 0 0.0198 6. 99 3E 04 0 . 0 1 8 7 0.0465 14.029 6 7.8854 0.000 025 0.0000 17 23.667 8 15.738 2 23.65 76 15.74 43 23.648 5 15.756 2 7.9296 7.913 3 7.8923 0.000 002 1. 75 7E 04 21.915 0.0000 84 0.0003 19 7.7 24 1 7.8 89 5 7.9 11 7 0.0003 78 Part C True Value Accurate Measure Discussio n Part A : • The analysis showed that the burette showed reliable results when compared to the other volumetric instruments having the lowest deviation from the means and an acceptable confidence level of <0.05. • The pipette in this experiment was the least accurate, having highest deviation when comparing means and an unacceptable confidence level. This result defies convention of the accuracy of the pipette and therefore the results suggest human error may have resulted in this disparity. • The measuring cylinder provided a reasonable accurate measure of Princes Town West Secondary Unit 2 Chemistry Labs 27 | P a g e volume in the Laboratory. Part B • Comparing the accuracy of various Classes of volumetric apparatus it was clearly seen that Class A apparatus showed a high degree of accuracy when compared to Class B apparatus, which is expected by convention. The confidence level for the Class A was less than that of the Class B indicating the reliability in the results. Part C • The accuracy of Group experiment showed that Group 1 was the closest to the accurate value whilst the rest of the group even though were precise were not accurate. Part D • When comparing the analytical balance to the top loading balance it was found the analytical balance was more accurate in its measure of weights as expected by convention. Conclusio n : The experiment was conducted successfully. An understanding of measurements accuracy and precision was clearly exemplified. Princes Town West Secondary Unit 2 Chemistry Labs 28 | P a g e Lab # 6 Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Crystallization Aim : To determine the number of moles of water of crystallization in hydrated MgSO4.xH2O Theory Hydrated salts contain water of crystallization which can be removed by : heating. The hydrated salt is heated and weighed repeatedly until a constant mass is obtained. This loss in mass of the salt can then be used to calculate the number of moles of water of crystallization. Apparatus and Material Procedure : : 1. Hydrated Magnesium sulphate MgSO4.xH2O 2. Hard glass test tube and holder 3. Balance 4. Bunsen burner 1. The empty hard glass test tube was weighed and this value was recorded. 2. 6 g of hydrated magnesium sulphate was then placed in the test tube and reweighed. Readings were recorded. 3. The test tube was then heated gently initially and then more strongly to remove any water that was present. Heating was stopped if the salt started to decompose or emit white fumes. 4. The test tube was allowed to cool and then the tube and its contents were reweighed and this value was recorded 5. The heating, cooling and weighing process was repeated until a constant mass was obtained. All the readings were recorded. Princes Town West Secondary Unit 2 Chemistry Labs 29 | P a g e Table 1 Mass of hydrated magnesium sulphate Mass of test tube and hydrated magnesium sulphate / g Mass of empty test tube /g Mass of hydrated magnesium sulphate /g Table 2 Observation s and Results Mass of hydrated magnesium sulphate after heating Mass of test tube and hydrated magnesium : sulphate after heating / g Mass of empty test tube /g Mass of hydrated magnesium sulphate after heating /g Data Analysis and Interpretati on : 1. Number of moles of anhydrous MgSO4 in the sample 2. Determine the mass of water driven out of the sample 3. Number of moles of water driven out from the sample 4. Number of moles of water of crystallization in one mole of hydrated MgSO4 = number of moles of water driven out from the samplenumber of moles of anhydrous magnesium sulphate in the sample 5. Write an equation for the formation of anhydrous MgSO4 from hydrated magnesium sulphate. Discussion : • Princes Town West Secondary Unit 2 Chemistry Labs 30 | P a g e Conclusion : Princes Town West Secondary Unit 2 Chemistry Labs 31 | P a g e Lab # 7 Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Analytical Chemistry : Gravimetric Analysis Aim : Standardisation of a permanganate solution and determination of the percentage oxalate in an unknown sample Apparatus and Material : Procedure : 1. 4 – 250 mL conical flask 2. Burette and funnel 3. Retort stand and clamp 4. 1 white tile 5. 2 sample vials with stoppers 6. Bunsen burner/ tripod / wire gauze 7. Thermometer 8. 25 mL measuring cylinder 9. 10 mL measuring cylinder 10. 25 mL Pipette and pipette filler 11. 2 spatulas 12. Analytical balance 13. K2C2O4.H2O salt 14. 3.0 M H2SO4 15. ≈0.02 M KMnO4 solution 16. Distilled water 17. Unknown oxalate sample 18. 250 mL volumetric flask 19. Laboratory cloth 1. ~1.5 grams of K2C2O4.H2O salt (Potassium oxalate) was accurately weighed and dissolved properly into a 250 ml volumetric flask and then made up to the mark. 2. 25 ml of K2C2O4 standard was pipette into a conical flask and 15 mL of 3.0 M H2SO4 was added. 3. The mixture was heated to about 85°C with a Bunsen burner and ensuring it did not came to a boil temperature. The temperature was monitored with a clean thermometer. 4. Using a laboratory cloth to hold the flask the solution was titrated against permanganate with constant swirling until the pink colour of the permanganate begins to take a few seconds to fade. 5. The temperature was ensured to be about 70 °C before continuing to titrate drop-wise to reach the end point which was a pale pink Princes Town West Secondary Unit 2 Chemistry Labs 32 | P a g e colouration that persists. 6. The titration was repeated twice more to ensure reliability of the titre value. 7. The mass of the unknown was accurately weighed and dissolved in 50 mL of distilled water in a 250 mL Volumetric flask and then made up to the mark 8. 15 mL of 3M H2SO4 was added to the unknown oxalate solution and titrated with the standardised KMnO4 as outlined in procedure # 2 to 6. 9. The mass of the unknown was repeatedly weighed until the mass produced a titre value close to standardisation value of the permanganate. Mass of K2C2O4.H2O salt used: ________ g Table: 1 Standardisation of KMnO4. Roug h : 1 2 3 Averag e Final Burette Reading / cm 3 Initial Burette Reading / cm 3 Observatio ns and Results Total Vol of Burette / cm3 Table: 2 Determination of the unknown concentration of oxalate. Roug h 1 2 3 Mass of unknown used /g Final Burette Reading / cm 3 Initial Burette Reading / cm 3 Total Vol of Burette / cm3 Data Analysis : 1. Calculation of the Molarity of the permanganate solution 2. Calculation of the percentage oxalate in the unknown mixture : 3. Why the unknown was repeatedly weighed to produce a titre value close to that of the permanganate. Princes Town West Secondary Unit 2 Chemistry Labs 33 | P a g e Discussion : • • • Conclusion : • • Princes Town West Secondary Unit 2 Chemistry Labs 34 | P a g e Lab # 8 Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Analytical Chemistry : Gravimetric Analysis Aim : To determine the Ascorbic Acid concentration in a commercially available Vitamin C Tablet. Theory VITAMIN C ANALYSIS Step 1: Potassium iodate reacts with iodide to form the complex trihalide I3- : IO3-+ 8I-+ 6H+yields 3I3- + 3H2O Anion Step 2: The trihalide ion reacts with vitamin C : : C6H8O6+ I3-yieldsC6H6O6 + 2H++ 3I- Step 3: Remaining trihalide is determined with thiosulfate I3-+ 2S2O32- yields 3I- + S4O62- Apparatus and Material : 1. 3. 2. 1 Wash bottle with distilled water 2 White tiles 2 100 mL and 2 250 mL 4. 2 Spatulas Beakers Princes Town West Secondary Unit 2 Chemistry Labs 35 | P a g e 5. 7. 9. 11. 13. 15. 17. 19. 21. 23. 2 sample vials with stoppers 6. 3 25 mL Pipettes and 3 pipette fillers 8. 2 25 mL, & 2 — 10 mL Measuring cylinders 1 250 mL, Volumetric flask 6 - 250 mL conical flasks 2 — 50 mL Burettes and 2 funnels Cotton wool, tissue & lab cloth 3 Filter funnels 1 Mortar and pestle 2 Pasteur pipettes 2 Retort stands and 10. 12. 14. 16. 18. 20. 22. Analytical & top loading balances 1000 mg vitamin C tablet in vial KIO3 solution (2.1526 g L -1) M H2SO4 Sodium thiosulfate solution 10% KI solution Starch clamps 24. 1 Wash bottle with distilled water 1 Glass rod Procedure : PROCEDURE A: Standardization of Sodium Thiosulfate Solution Using Iodate Pipette 25 mL of the standard potassium iodate solution provided into a 250 mL Princes Town West Secondary Unit 2 Chemistry Labs 36 | P a g e conical flask. Add 15 mL of 1 M sulfuric acid to the flask followed by 10 mL of 10% potassium iodide solution. Titrate the liberated iodine against the sodium thiosulfate solution until the solution changes to a pale yellow. Then add 10 drops of starch solution and continue the titration until the blue colouration just disappears. PROCEDURE B: Determination of Ascorbic Acid content of Vitamin C Tablet Accurately weigh and record the mass of one Vitamin C tablet. Using a mortar and pestle pulverize the entire tablet. Transfer 500 mg of the powder (accurately weighed) to a 100 mL beaker and add 75 mL of 1M H 2SO4. Agitate the mixture with a glass rod and gravity filter into a 250 mL volumetric flask. To filter the solution plug the stem of a funnel with a piece of cotton wool. Rinse the cotton with a small volume of 1M H 2SO4 ensuring that the flow rate is moderate before beginning to filter the Vitamin C — H2SO4 mixture. Ensure that no solid material goes into the volumetric flask. If this happens you will need to re-filter. Make the solution up to 250 mL with 1M H 2SO4. Pipette 25 mL of the vitamin C solution into a conical flask and add 25 mL of standard KIO 3 solution by pipette. Add 10 mL of 10% KI solution to the mixture and titrate against the standardized thiosulfate solution until the solution becomes a very pale yellow. Add 10 drops of starch solution and continue the titration until the blue colouration just disappears. Repeat the experiment until a satisfactory average is obtained for the end point. Observatio ns and Results : Using a Suitable Tables Record Your Results. Data Analysis Discussion Use your average titre volume to calculate 1. The number of moles of 10; initially added to the solution :2. The number of moles of I; produced initially 3. The number of moles of I; which reacted with thiosulfate 4. The number of moles of I; which reacted with L-ascorbic acid : • • • Princes Town West Secondary Unit 2 Chemistry Labs 37 | P a g e Conclusion : • • • Princes Town West Secondary Unit 2 Chemistry Labs 38 | P a g e Lab# 9 Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Analytical Chemistry : Spectroscopy : UV/Vis Aim : To determine the nitrate concentration in an unknown water sample using UV/vis spectroscopy and the use of Beer Lamberts Law. Apparatus and Material 5. 50 cm3 burette 6. Cadmium reduction mixture 7. Test tubes 8. Stopwatch : 1. Evolution 600 UV-Vis Spectrophotometer) 2. 10 ppm KNO3solution (100 mg dm3 anhydrous KNO3) 3. 5 – 50 cm3 volumetric flask 4. Unknown water sample : 1. Using a burette serial dilutions were made of the KNO3 Standard by transferring 10, 20, 30, 40, and 50 cm3 of KNO3 into their respective volumetric flask. Procedure 2. Each flask was then made up to the mark using distilled water and labelled 2, 4, 6, 8, and 10 ppm respectively. 3. 5 cm3 of each standard was placed into a test tube and labelled, together with the blank which was distilled water and labelled (0 ppm) and the unknown sample. 4. A spatula of cadmium reduction mixture was added to each test tube and timed for 12 minutes for each reduction. 5. After the reduction the samples were then measured for Absorbance on the UV/Vis spectrophotometer at λ= 530 nm. 6. The results were tabulated and a standard curve was drawn. Princes Town West Secondary Unit 2 Chemistry Labs 39 | P a g e Table: 1 Observatio ns and Results : NO3- / ppm 0 2 4 6 8 10 Unknow n Absorbanc e 0 0.053 0.1157 0.216 7 0.230 9 0.2622 0.1696 1. Plot a graph of Absorbance versus concentration of NO3- Graph Data Analysis Absorbance values of nitrate ions standards and unknown : 2. From the graph infer the unknown concentration of the unknown sample 3. Using Beer-Lamberts Law A=εcl determine the concentration of the unknown sample 4. When the c = 12 ppm A= ? l = 0.5 cm determine the Absorbance Discussion : • • • • Conclusion : • Princes Town West Secondary Unit 2 Chemistry Labs 40 | P a g e Lab# 10 Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Analytical Chemistry : Quantitative Titration Aim To determine the concentration of iron(II) ions in an unknown solution and, by a • graphical method, : • the volume of an aqueous solution of substance X that will oxidize the iron(II) ions in 25 cm3 of the unknown. 1. A is 0.02 mol dm-3 potassium manganate(VII), KMnO4 2. B is the unknown containing iron(II) ions, Fe2+, 3. C is an aqueous solution of a substance X. 4. Under acid conditions X oxidizes iron(II) to iron(III). Apparatus and Material : Procedure : Experiment 1 1. Fill a burette with A, 0.02 mol dm-3 potassium manganate(VII), KMnO4. 2. Pipette 25.0 cm3 of B into a conical flask and add, using the measuring cylinder provided 10 cm3 of 1 mol dm-3 sulphuric acid. 3. Run A from the burette into the conical flask until the first permanent pale pink colour remains. This is the end point of the titration. 4. Record your burette readings in Table 1. 5. Repeat the titrations as many times as you think necessary to obtain accurate results. 6. Make certain that the recorded results show the precision of your Princes Town West Secondary Unit 2 Chemistry Labs 41 | P a g e practical work. Experiment 2 1. Fill the second burette with C, the aqueous solution of X. 2. Pipette 25.0 cm3 of B into a conical flask and add, using the measuring cylinder provided, 10 cm3 of 1 mol dm-3 sulphuric acid. 3. Add, from the second burette, 4.00 cm 3 of X. This oxidizes some of the Fe2+ that has been pipetted into the flask. 4. Titrate the remaining Fe2+ in the conical flask with A, potassium manganate(VII) until the first permanent pale pink colour remains. 5. Record the volume of X added and your burette readings in table 2. 6. One accurate titration will be sufficient. Remember that the volume added will be less than in Experiment 1 as some of the Fe 2+ has been oxidized by X. 7. Empty and rinse the conical flask. 8. Repeat Experiment 2, using the volumes of X shown in Table 2. 9. Record your results in Table 2. Table 1: Titration of A, 0.02 mol dm-3 potassium manganate(VII), KMnO4 with B, the unknown. Final burette reading/ cm3 Observatio ns and Results : Initial burette reading/ cm3 2 2 2 5 6 5 . . 5 2 4 0 0 0 0 1 1 . . 5 0 0 0 0 0 . . 28.80 3.60 Volume of A used/ cm3 Princes Town West Secondary Unit 2 Chemistry Labs 42 | P a g e Table 2 Titration of B/X mixture with A, KMnO4 0 4 8 Volume of X added/ 3 cm . . . 0 0 0 12.00 0 0 0 2 2 Final burette reading/ cm3 0 2 . . 20.00 0 5 0 0 Initial burette reading/ cm3 0 6 . . 8.05 0 5 5 0 Volume of A added/ cm3 ↑ Copy and fill out the titration values Data Analysis : Experiment 1 (a) 25.0 cm3 of B reacted with …………….. cm3 of A. Show which results you used to obtain this volume of A by placing a tick under the readings in Table 1. (b) Calculate how many moles of potassium manganate(VII) were run from the burette into the conical flask during the titration of B with A. Princes Town West Secondary Unit 2 Chemistry Labs 43 | P a g e (c) Use the half equations for the reaction MnO4aq- + 8Haq+ + 5e- yields Mnaq2+ + 4H2Ol Fe(aq)2+ yields Fe(aq)3+ + eand your answer to (b) to calculate the concentration of Fe2+, in mol dm-3, in B. Experiment 2 (a) Plot the volume of A against the volume of X. a. Your scale on the X axis should extend to 30.00 cm3. (a) Draw the best-fit straight line through the plotted points. (b) From your graph find the volume of X that reacts with the Fe2+ present in 25.0 cm3 of B. Graph Discussion : • • • • Conclusion : • Princes Town West Secondary Unit 2 Chemistry Labs 44 | P a g e Lab# 11 Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Chromatography Theory In this activity you will be experimenting with a technique called chromatography which will allow you to visually demonstrate that the pigment in leaves is a combination of several different coloured pigments. : This technique is useful in that it can separate and identify the various components of mixtures, such as those contained in plant pigments. A pigment is a substance that absorbs light at specific wavelengths, chlorophyll is one of these pigments. Its green-yellow in colour is due to the absorption of red, orange, blue, and violet wavelengths and the reflection of the green and yellow wavelengths. This occurs when white light (containing all of the light wavelengths, or the entire spectrum of colours) shines on the leaf surface, all of the wavelengths are absorbed except for the ones you see, which are green-yellow, those are the portions of the spectrum being reflected. If the conditions are identical, the relative distance moved by a particular compound is the same from one mixture to another. This is why chromatography can be used to identify a compound. The actual identification requires a simple calculation as shown below: Rf=Distance travelled by componetDistance travelled by mobile phase It is important to remember that several factors can influence the reliability of the Rf value, these include humidity, temperature, solvent, pigment extract preparation, and the amounts of the material present. Values are comparable only when the extracts are prepared in the same way and the chromatograms are prepared identically and developed together in the same container. Acetone is flammable (even the amount found in nail polish remover), keep it away from sparks or open flames. Wear eye protection, especially if using pure acetone. Aim : To separate the different pigments in plant leaves Apparatus : 1. Cone-type (size 4) Princes Town West Secondary Unit 2 Chemistry Labs 5. capillary tubes 45 | P a g e and Material Procedure coffee filter paper (or Whatman #1 chromatography paper) 2. large glass jars 3. acetone 4. distilled water 6. fresh spinach 7. mortar and pestle 8. clean sand 1. Each lab group (or individual if not working in groups) will need 4 strips of filter paper, approximately 6 inches long and 1 inch wide, 2 chromatography development containers (500 ml beakers or large fruit : jars work well), 2 large rubber bands (able to stretch around the vessels from the mouth to the bottom of the vessel), 2 solvents, water and either pure acetone, or nail polish remover. 2. Do the following with both fresh spinach leaves; tear leaf material and place in a glass container, cover with acetone (this should be done the day before the actual lab activity). An alternative pigment extraction technique is to use a mortar and pestle. Place plant material the vessel, add a little clean sand, some acetone and then grind until a dark green liquid appears. Both techniques yield very dark pigments with which to work. Be certain to keep the pigments apart throughout the entire activity. 3. Place one of each solvents (water and acetone, or nail polish remover) in the chromatography vessels and stretch a rubber band length-wise around each vessel. The rubber band will be the mechanism for hanging the chromatography strips. 4. Make a pencil mark on each of the 2 chromatography strips, in the center, directly above the point of the strip, about 1 inch from the tip of the paper. Using a capillary tube, or tooth pick, apply the plant pigment to each filter paper strip. This is done by touching the tooth pick or capillary tube which has been dipped in the pigment, to the pencil mark. Make an application, then wave the paper gently to dry it a little before the next application. Be patient, you will need 12 to 15 applications. 5. By now you should have 2 strips with spinach pigment. Suspend one of each in each of the chromatography development vessels. You can attach them with paper clips, or simply fold over a portion of the end and it should hang in place. The tip of each strip should just touch the solvent. 6. Wait 20 to 30 minutes for the chromatograms to develop. Remove the chromatograms. Mark with a pencil (NOT a pen) where the solvent stopped as it moved up the chromatogram. This is called the solvent front. Mark also where each pigment stopped moving up the chromatogram. Using the equation below, determine a reference number for each pigment on the chromatograms. Depending on which chromatogram you are viewing, you should see greens, yellow/yellow orange, and red. All measurements should be in mm. (Any material which did not move from the pencil dot is insoluble). Princes Town West Secondary Unit 2 Chemistry Labs 46 | P a g e Observatio ns and Results Note: each pigment has a special name, green = chlorophyll a or b : yellow/yellow orange = carotene red = anthocyanin brown = xanthophyll 1. Determine the Rf (ratio of fronts) value for each spot using the formula (remember there may be more than one calculation per spot): Data Analysis : Rf=Distance travelled by componetDistance travelled by mobile phase Discussion : • • • • Conclusion : • Princes Town West Secondary Unit 2 Chemistry Labs 47 | P a g e Lab# 12 Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Chromatography Aim : To determine the various colours present in food colouring dye and ink pens using chromatography. Apparatus and Material 1. 2. : 3. 4. 5. Procedure : 8" Aluminum pie plate Toothpicks Food coloring Large Coffee filters Pencil 6. 7. 8. 9. Rubbing alcohol (70% Isopropyl Alcohol) "Expresso" ink pens Measuring cup Ruler 1. Measure approximately 1cm from the edge of the coffee filter and mark eight evenly spaced lines around the filter lightly with a pencil (do not use ink). 2. Use a toothpick to spot the four food colouring dyes on one-half of the coffee filter along the pencil mark. On the other half, spot the filter using the pens. Allow each spot to dry and then re-spot two or three more times 3. After the coffee filter has been spotted, mark the top of each spot with your pencil. Below each spot, in pencil, label the colour and if it is pen or food colouring. (Due to the small space below the spot, it may be easier to label "p" for pen, "g" for green, etc.) 4. Place the spotted coffee filter "upside down" in the aluminium pie plate. 5. Fill the pie plate (the chromatographic chamber) with rubbing alcohol to a level just below the spots on the filter. 6. Once the chromatogram has developed and the alcohol travels to the "top" of the coffee filter, remove the filter from the plate. Using a pencil, Princes Town West Secondary Unit 2 Chemistry Labs 48 | P a g e mark the final position of the solvent. Click here to view a video of this procedure. 7. Next place a pencil mark at the end of each colour. Note that some of the initial spots separated into multiple colours- be sure to mark each colour 8. Allow the filter to air dry. 9. Determine and record the distance travelled by the mobile phase by measuring the distance (in mm) from the top of each of the initial spots (original pencil marks) to the final position of the solvent 10. Determine and record the distance travelled by each colour by measuring the distance (in mm) from the pencil mark at the top of each of the initial spots to the mark indicating the end of each colour it produced. Observatio ns and Results : 1. Determine the Rf (ratio of fronts) value for each spot using the formula (remember there may be more than one calculation per spot): Data Analysis : Rf=Distance travelled by componetDistance travelled by mobile phase Discussion : • • • • Princes Town West Secondary Unit 2 Chemistry Labs 49 | P a g e Conclusion : • Princes Town West Secondary Unit 2 Chemistry Labs 50 | P a g e Lab# 13 Skills Assessed: Date: O/R/ R M/M A/I P/D Title Theory : Solvent Extraction Introduction: The aim of this experiment is to determine the equilibrium constant, K, for the distribution of ethanoic acid (CH3COOH, also known as acetic acid) between two immiscible solvents, diethyl ether and water, using an acid-base titration. The dynamic equilibrium at the solvent boundary may be represented by eq. 2 [CH3COOH](aq) k1⇋ k2 [CH3COOH]ether eq.2 : where k1 and k2 are the rate constants of the forward and reverse processes. At equilibrium the rates of the forward and reverse processes are equal (eq. 3), k1[CH3COOH](aq)=k2[CH3COOH](ether) eq.3 and the equilibrium constant may then be defined in terms of solute concentrations or rate constants according to eq. 4. K=[CH3COOH]ether[CH3COOH]aq=k1k2 eq.4 Aim To determine the equilibrium constant, K, for the distribution of ethanoic acid : (CH COOH, also known as acetic acid) between two immiscible solvents, diethyl 3 ether and water, using an acid-base titration. Apparatus : 1. Graduated 100 cm3 measuring Princes Town West Secondary Unit 2 Chemistry Labs 6. Distilled water; 51 | P a g e 2. 3. 4. 5. and Material Procedure : 7. Diethyl ether; 8. 0.5 M aqueous ethanoic acid; 9. 0.125 M aqueous ethanoic acid; 10. Phenolphthalein indicator solution. 1. Using a measuring cylinder, add as accurately as possible 50 cm 3 of 0.5 mol dm 3 2. 3. 4. 5. 6. Observatio ns and Results cylinder; 250 cm3 separating funnel; Three 100 cm3 conical flasks; 10 cm3 pipette; 50 cm3 burette; 0.1 M sodium hydroxide solution; aqueous ethanoic acid and 50 cm 3 of diethyl ether into a 250 cm 3 separating funnel. Allow the mixture to stand for at least twenty minutes, shaking occasionally (remember to release the pressure after shaking by inverting the funnel, holding on to the stopper and opening the tap). Run off the lower (aqueous) phase into a clean 100 cm 3 conical flask, making sure no ether is drained off. Now run off the interface portion and discard it and leave the ether layer phase in the separating funnel. Pipette 10 cm3 of each phase into separate 100 cm 3 conical flasks and add a few drops of phenolphthalein indicator. Titrate both solutions with 0.1 mol dm -3 sodium hydroxide solution until the solution retains a permanent pink colour. Repeat the titrations with two further 10 cm 3 aliquots. Repeat steps 1-3 using 0.125 mol dm -3 aqueous ethanoic acid in place of the 0.5 mol dm-3 solution. : Data Analysis Treatment of Results 1. Write a balanced equation for the reaction between ethanoic acid and sodium hydroxide. 2. From the volumes of sodium hydroxide solution required to neutralise the ethanoic acid, calculate the number of moles of ethanoic acid in each 10 cm 3 aliquot you titrated and, hence, evaluate the ethanoic acid concentration for each : solution. 3. Using the ethanoic acid concentrations you have determined, evaluate the equilibrium constant, K, using eq. 4 for both initial ethanoic acid concentrations. 4. Do the values of K obtained using 0.5 mol dm -3 and 0.125 mol dm -3 ethanoic acid differ? Is this to be expected? Discussion : • • Conclusion : • Princes Town West Secondary Unit 2 Chemistry Labs 52 | P a g e Princes Town West Secondary Unit 2 Chemistry Labs 53 | P a g e Lab # 14 Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Plan Design: Analytical Chemistry Case Contamination of drinking water supplies from industrial waste is a result of various types of industrial processes and disposal practices. Industries that use large amounts of water for processing have the potential to pollute : waterways through the discharge of their waste into streams and rivers, or by run-off and seepage of stored wastes into nearby water sources. From this information, is it possible to determine if effluents from ISPAT is sufficient to cause adverse effects on the quality of drinking water. Hypothesis : Aim : : 1. Apparatus and Material : Procedure : 2. 1. 2. 3. 4. Variables : Princes Town West Secondary Unit 2 Chemistry Labs 54 | P a g e Expected Observatio ns and Results : Data Analysis : 1. 2. Precaution s : Princes Town West Secondary Unit 2 Chemistry Labs 55 | P a g e Lab # NA Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Organic Chemistry Aim : To distinguish between alkanes and alkenes Alkanes are saturated compounds which are relatively inert. They undergo very few reactions which include combustion, cracking and free radical substitution with halogens such as chlorine and bromine. Substitution reaction requires the presence of UV light which is needed to split the inert halogens to from the reactive free radicals. Alkenes on the other hand, are unsaturated compounds which takes part in a wide range of addition reactions due to the presence of the double bond in alkene molecules. The double bonds in alkenes are an electron rich centre which allows it to undergo electrophilic reactions and react with oxidizing agents. Theory Alkenes decolorize a solution of bromine in 1, 1, 1 trichloroethane : CH3CCl3 C6H12 + Hexene Br2 C6H12Br2 reddish – brown colourless 1,2 dibromohexane Alkenes decolorize an acidified solution of potassium manganate (VII) KMnO4/H+ C6H12 + Hexene C6H12(OH)2 colourless hexane – 1,2 - diol Princes Town West Secondary Unit 2 Chemistry Labs 56 | P a g e Apparatus and Material Procedure : 1. 1 % bromine in 1,1,1 trichloroethane 2. 0.02 mol dm-3 KMnO4 3. Dilute H2SO4 4. Samples of Hexane and Hexene labelled A and B 5. Test tubes 1. To 2 cm3 of sample A in a test tube, bromine was added drop by drop. Solution was stirred and observations were made and recorded. : 2. A few drops of dilute H2SO4 was added to 1 cm3 of KMnO4. The acidified KMnO4 was then added drop by drop to another test tube containing 2 cm3 of sample A. The mixture was stirred and observations were made and recorded. 3. Steps 1 and 2 were then repeated with sample B Table 1 Observation s and Results Data Analysis and Interpretati on Observation table Step Sample A Sample B : On addition of Bromine Solution turns colourless No visible reaction On addition of acidified KMnO4. Solution turns colourless No visible reaction 1. Deduce which sample is an alkane and which is an alkene : Sample A is an Alkene and Sample B is an Alkane. 2. Outline the mechanisms for any reactions which occurred. Alkenes contain a double bond, which is an electron rich centre, and in the presence of bromine in 1, 1, 1-trichloroethane, Br2 is added across the double bond, causing decolourization of the bromine solution from brown to colourless, via an addition reaction. CH3CCl3 C6H12 + Hexene Br2 reddish – brown Princes Town West Secondary Unit 2 Chemistry Labs C6H12Br2 colourless 1,2 dibromohexane 57 | P a g e Alkenes react with potassium manganate(VII) solution. If the potassium manganate(VII) solution is acidified with dilute sulphuric acid, the purple solution becomes colourless. Manganate(VII) ions are a strong oxidising agent, and they will oxidise the hexene to a diol, while the manganate(VII) ions are reduced to manganese(II) ions. Manganate (VII) ions are purple in solution whereas manganese(II) ions are colourless, and hence there will be a colour change from purple to colourless in the presence of the alkene. KMnO4/H+ C6H12 + C6H12(OH)2 Hexene colourless hexane – 1,2 – diol Neither of these reactions will occur with the alkane, and so there will be no colour change in the presence of the alkane. Conclusion : Sample A is Hexene and Sample B is Hexane Princes Town West Secondary Unit 2 Chemistry Labs 58 | P a g e Lab # NA Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Carbonyl Compounds Aim : Investigating Carbonyl Compounds Aldehydes and ketones are carbonyl compounds which contains the Theory carbonyl group . Aldehydes have a single alkyl or acyl group attached to the carbon atom whilst ketones have two. They both react with 2, 4 dinitrophenylhydrazine (2,4 – DNPH) to give orange precipitates. : Mild oxidising agents such as Tollen’s and Fehling’s reagents can oxidise aldehydes but not ketones. Hence this reaction can be used to distinguish the both of them. Tollen’s reagent forms a silver mirror on the wall of the test tube and Fehlings solution gives a red-brown precipitate of Copper (I) Oxide. Three samples are given, two of which are carbonyl compounds, one is an aldehye and the other is a ketone. Apparatus and Material Procedure : 1. Samples B, C and D 2. 2, 4 – DNPH (dissolve 2 g of 2, 4DNPH in 4 cm3 of conc. H2SO4, 30 cm3 of methanol and 10 cm3 of water. : 1. 3 cm3 of 2,4 – DNPH was added to a few drops of sample B in a test tube and observations were made and recorded. Princes Town West Secondary Unit 2 Chemistry Labs 3. Fehling’s reagent 4. Aqueous NH3 and AgNO3 5. Measuring cylinders 6. Test tubes 59 | P a g e 2. To 3 cm3 of AgNO3 in a test tube, ammonia solution was then added dropwise, while shaking the solution until the precipitate formed redissolved. A few drops of sample B was then added to this tube (Tollens Test) and observations were made and recorded. 3. A few drops of sample B was added to Fehlings reagent in a test tube and the tube was gently heated. Observations were recorded. 4. Steps 1-3 was then repeated for Sample C and D. Table 1 Observation s and Results Data Analysis and Interpretati on : Observations Solution added Sample B Sample C Sample D 2,4- DNPH Orange precipitate formed Orange precipitate formed No visible reaction AgNO3 and NH3 No visible reaction Silver mirror formed on test tube No visible reaction Fehlings reagent No visible reaction Redbrown precipitate No visible reaction 1. Deduce which of the samples are carbonyl compounds. : Sample B and Sample C are both Carbonyl compounds since they both reacted with 2, 4 dinitrophenylhydrazine to give an orange precipitate. 2. Deduce which of the samples is an aldehyde Sample C is an aldehyde since it was oxidised by both Tollen’s reagent and Fehlings reagent where it formed a silver mirror and a red- brown precipitate respectively. Mild oxidising agents can oxidise aldehydes, but not ketones. Tollens Test: CH3CHO(l) + 2Ag+(aq) + H2O(l) CH3COOH (aq) + 2Ag(s) + 2H+(aq) Fehlings reagent gives a red brown precipitate of copper (I) Oxide: Princes Town West Secondary Unit 2 Chemistry Labs 60 | P a g e CH3CHO(l) + 2Cu2+(aq) + 2H2O(l) CH3COOH (aq) + Cu2O(s) + 4H+(aq) 3. Deduce which sample is a ketone: Sample B is the ketone, since it gave an orange precipitate with 2, 4 dinitrophenylhydrazine, which means that it is a carbonyl compound, but it showed no reaction with the weak oxidising agents Tollens reagent and Fehlings reagent. These two tests are used to distinguish between an aldehyde and a ketone. Discussion • : • • • Conclusion : Tollens reagent and Fehlings reagent are weak oxidising agents Can be used to distinguish between aldehydes and ketones Shows not reaction with ketones Gives a silver mirror test and red-brown precipitate with aldehydes Sample B is the ketone and Sample C is an aldehyde. Princes Town West Secondary Unit 2 Chemistry Labs 61 | P a g e Lab # NA Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Organic Compounds Aim : To investigate the oxidation of alcohols Primary alcohols are readily oxidised to carboxylic acids by a variety of oxidising agents such as acidified potassium dichromate (VI). Theory Cr2O72- (aq) + 14H+(aq) + 6e: 2Cr3+ (aq) + 7H2O(l) Orange green Primary alcohols are firstly oxidised to aldehydes and then to carboxylic acids. Secondary alcohols are oxidised to ketones and tertiary alcohols are resistant to oxidation. Apparatus and Material : Procedure : 1. Ethanol 2. 5 g dm-3 Potassium dichromate (VI) solution 3. Test tubes 4. Water bath 5. Concentrated sulphuric acid 6. Measuring cylinders 7. Bunsen burner and tripod stand 1. 5 cm3 of ethanol was placed in a test tube. 2. To this tube, 2 cm3 of potassium dichromate (VI) solution was added, followed by 3-4 drops of concentrated H2SO4 3. The tube containing this mixture was then placed in a water bath at 60 ºC and any colour change was recorded. Princes Town West Secondary Unit 2 Chemistry Labs 62 | P a g e 4. The mixture was then allowed to cool and the scent of vinegar was identified coming from the reaction mixture. Table 1: Observations Observation s and Results On addition of potassium dichromate (VI) solution : On addition of concentrated H2SO4 and heat Solution in test tube turned orange colour Colour change from orange Light green After cooling yellow blue The scent of vinegar was noted and two layers were present in the tube, one clear and the other cloudy 1. Write equations for any reactions that occurred Data Analysis and Interpretati on An acidified dichromate ion readily accepts electrons and this makes it a powerful oxidising agent. Cr2O72- (aq) + 14H+(aq) + 6eOrange : 2Cr3+ (aq) + 7H2O(l) green Initially, the colour of the solution is orange due to the dichromate ions. On oxidation of ethanol, which is a primary alcohol, an aldehyde is formed firstly. Cr2O72-/H+ CH3CH2OH(aq) CH3HC=O + 2H+ + 2eThe aldehyde is further oxidised to a carboxylic acid, ethanoic acid. CH3HC=O + H2O CH3COOH + 2H+ + 2e- 2. Describe how this experiment can be scaled up to obtain a pure sample of ethanoic acid. Princes Town West Secondary Unit 2 Chemistry Labs 63 | P a g e Discussion : • Discuss the principles on which the breathalyser test is based?. Potassium dichromate, K2Cr2O7, is a yellow-orange compound. When alcohol vapour makes contact with the yellow-coated crystals, the colour changes from yellow to green. The degree of the colour change is directly related to the level of alcohol in the suspect's breath. The oldest breathalyzer models pass breath through a solution of potassium dichromate, which oxidizes ethanol to acetic acid, changing color in the process Cr6+ Orange Conclusion Cr3+ Green : Princes Town West Secondary Unit 2 Chemistry Labs 64 | P a g e Lab # NA Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Organic Compounds Aim : To investigate the formation of esters When an alcohol reacts with a carboxylic acid in the presence of concentrated sulphuric acid, an ester is formed. Theory : RCOOH(l) + R OH(l) RCOOR (l) + H2O (l) The ester is recognised by its distinctive fruity smell. Apparatus and Material Procedure Ethanol Glacial ethanoic acid Cold water Bunsen burner and tripod Boiling tube 6. Concentrated sulphuric acid 7. Measuring cylinders 8. Beaker 9. Water bath : 1. 2. 3. 4. 5. : 1. 5 cm3 of ethanol and 5 cm3 of ethanoic acid were placed in a boiling tube. 2. To this tube, 1 cm3 of concentrated H2SO4 was then added. 3. The tube containing this mixture was then placed in a water bath at 60 ºC and observations were made. 4. After heating the mixture in the water bath for 10 minutes, it was then poured into a beaker containing ice cold water. Observations Princes Town West Secondary Unit 2 Chemistry Labs 65 | P a g e were made and recorded. Table 1: Observations Observation s and Results On mixing ethanoic acid and ethanol : On addition of concentrated H SO and heat 2 4 On pouring mixture in beaker containing ice cold water Data Analysis and Interpretati on No visible reaction No visible reaction Two distinct layers were visible. The top layer was insoluble and cloudy and a sweet fruity smell was emitted. 1. Write equations for any reactions that occurred : Ethanol and Ethanoic acid react together to from an ester, ethyl ethanoate CH3COOH(l) + CH3CH2OH(l) CH3COOHCH2CH3(l) + H2O(l) 2. What is responsible for the smell obtained. The formation of the ester, ethyl ethanoate, which has a fruity smell, is the compound that is responsible for the fruity smell emitted during the reaction. 3. Why is the reaction mixture poured into cold water? Discussion • • : Conclusion • Ethanoic acid + ethanol ethyl ethanoate + water ethyl ethanoate is a volatile ester with a characteristic fruity smell. Ester formation is an example of a condensation reaction, where there is elimination of water, and formation of the ester bond. : Princes Town West Secondary Unit 2 Chemistry Labs 66 | P a g e Princes Town West Secondary Unit 2 Chemistry Labs 67 | P a g e Lab # 15 Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Simple and Fractional Distillation Aim : To compare the efficiency of separation of an ethanol- water mixture by simple and fractional distillation. Theory Simple distillation is used to separate volatile liquids from non- volatile ones with different boiling points. If the boiling points difference between two : liquids is less than 60 degrees, then fractional distillation is used instead. Ethanol and water are miscible solvents whose boiling points are 78ºC and 100ºC respectively, and hence fractional distillation is more appropriate for their separation. Apparatus and Material : Procedure : 1. Simple and Fractional distillation apparatus 2. 50-50 mixture of ethanol and water 3. Thermometer 4. 50 cm3 measuring cylinder 5. Anti-bumping granules 6. Hot plate 1. The class was divided into two groups. One group carried out simple distillation and the other group carried out fractional distillation. The information and data obtained by both groups were then shared within the two groups. 2. The distillation apparatus was set up, using a measuring cylinder as the collecting vessel. 3. 50 cm3 of ethanol- water mixture together with some anti-bumping granules were added to the distilling flask. The flask was then attached to the rest of the apparatus. Princes Town West Secondary Unit 2 Chemistry Labs 68 | P a g e 4. The distilling flask was placed on a hot plate 5. The temperature was recorded when the first drop of distillate came out. 6. The temperature was recorded for every 3 cm3 of distillate that came out 7. Distillation was stopped when there was about 5 cm3 of mixture in the distilling flask. Observatio ns and Results : Table 1 : Temperature readings for distillate collected during fractional distillation Volume of Distillate / cm 3 Temperature / ºC 1st drop 03 06 09 12 15 18 21 24 27 30 Table 2 : Temperature readings for distillate collected during simple distillation Volume of Distillate / cm 3 Temperature / ºC 03 06 09 Princes Town West Secondary Unit 2 Chemistry Labs 69 | P a g e 12 15 18 21 24 27 30 1. Plot graph of temperature of distillate against volume of distillate for simple and fractional distillation on the same axes. Graph Data Analysis : 1. Use your graph to deduce which type of distillation separates the mixture better 2. Explain graph Data Analysis and : Interpretati on 1. Write a balanced equation for the reaction between hydrochloric acid and sodium hydroxide Discussion • How would heating more rapidly affect distillation? • What effect would using a longer column have on fractional distillation? Conclusion : : Princes Town West Secondary Unit 2 Chemistry Labs 70 | P a g e Lab # 16 Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Organic Compound Aim : To analyse substance X4 Theory : Sample X4 is an organic compound which contains four Carbon atoms. Apparatus and Material Procedure : : 1. 2. 3. 4. Sample X4 Bromine solution Solid Na2CO3 Test tubes and test tube racks 5. 2, 4 – DNPH 6. Bench reagents : AgNO3(aq), NH3(aq), NaOH(aq), I2(aq) 7. Measuring cylinder 1. Bromine solution was added dropwise to 3 cm3 of sample X4 and observations were made and recorded 2. Solid Na2CO3 was added to 3 cm3 of sample X4 and observations were made and recorded 3. 2, 4 – DNPH was added dropwise to 3 cm3 of sample X4 and observations were made and recorded 4. Ammonia solution was added dropwise to 4 cm3 of AgNO3 until the precipitate that formed just re dissolved. (Tollens Reagent). A few drops of Sample X4 was then added to this reagent. Any observations made were recorded. 5. 2 cm3 of Iodine solution was added to 3 cm3 of sample X4 . NaOH was then added dropwise while gently shaking. Any observations made were recorded. Princes Town West Secondary Unit 2 Chemistry Labs 71 | P a g e Table 1: Observations and Inferences made during the experiment Tests Observations Inferences On addition of Bromine solution Observatio ns and Results : On addition of Solid Na2CO3 On addition of 2,4 DNPH On addition of Tollens Reagent On addition of Iodine Solution Discussion : • Conclusion : • Princes Town West Secondary Unit 2 Chemistry Labs 72 | P a g e Lab # 17 Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Plan Design: To determine the acidity / pH of a soil sample Soil pH is a measure of hydronium ion (H3O+, or more commonly the H+) activity in the soil solution. Soil pH influences many facets of crop production and soil chemistry, including availabilities of nutrients and toxic substances, activities and nature of microbial populations, and activities of certain pesticides. Soils with pH values below 7 are referred to as "acid" and those with pH values above 7 as "alkaline"; soils at pH 7 are referred to as "neutral." Most crops grow best if the soil pH is between 6.0 and 7.5. Case : Mr. Smith, a farmer, wants to increase his yield of vegetables, but of lately, he finds that his plants are not thriving well. His neighbour tells him that his soil may be too acidic for the plants to grow well, and suggests that he test the pH of his soil to confirm. Plan and Design an experiment to test for the pH of the soil, using a pH meter. Apparatus and Material Procedure : : 1. 2. 3. 4. pH meter Electronic balance Paper cups Standard buffer solutions of pH 2, 4, 7 and 9 for calibrating pH meter 5. 6. 7. 8. Pipettes Soil sample Deionized water Stirrer 1. Calibrate the pH meter over the appropriate range using the standard buffer solutions. 2. Weigh 5 g of sieved, air dried soil sample and place into a paper cup. 3. Add 5 ml deionized water to the sample of soil in the paper cup Princes Town West Secondary Unit 2 Chemistry Labs 73 | P a g e 4. Stir the sample for a minute, and then leave to settle for 30 minutes to form a slurry 5. After formation of slurry, place electrodes of the pH meter into the slurry and read the pH, ensuring that the electrodes tips are in the slurry. 6. Repeat the above procedure at least twice for accuracy. Observatio ns and Results Discussion Conclusion Table 1 : Recorded pH of soil sample Samples : : pH Sample 1 Sample 2 Sample 3 • Which type of water (distilled, deionized, tap water) is preferred, and give reasons why? • Theoretically, if the pH of a soil sample is 2, and you want to obtain the optimum pH for best growth of crop, that is, a pH between 6.0 and 7.5, what procedures can be done to this soil sample in order to achieve this optimum pH? • Give two precautions that should be undertaken when carrying out your experiment? : Lab # NA Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Plan Design: To purify a sample of rock salt Case : Rock salt, also known as halite, or impure NaCl, is typically formed by the evaporation of salty sea water which contains dissolved Na+ and Cl- ions, as well as other impurities such as potassium and sulphates. Rock salt is also applied to Princes Town West Secondary Unit 2 Chemistry Labs 74 | P a g e road beds in cold climates to help reduce the freezing point of water on the road, and since saline has a lower freezing point that water, putting rock salt on ice will cause it to melt. Table salt is essential for human life. A large amount of the commercially mined rock salt is prepared for human consumption. Plan and design an experiment to obtain pure NaCl, or table salt, from a sample of impure rock salt. Include how you would be able to find the percentage of mass of salt crystals recovered from the sample of rock salt. Apparatus and Material : Procedure : 1. 2. 3. 4. Sample of rock salt Balance / Scale Filter paper and funnel Bunsen burner 5. 6. 7. 8. Conical flasks Distilled water Ice bath Beakers 1. Weigh 10 g of the rock salt using the electronic balance 2. Place this sample in a beaker, and then dissolve it in a small amount of hot water, while stirring the mixture 3. Weigh a sheet of filter paper and record this value 4. After dissolving the sample of rock salt, then filter the mixture, collecting the filtrate in a conical flask 5. Allow the filtrate in the conical flask to cool by placing in into an ice bath, to recrystallize the NaCl 6. Filter the crystals with distilled water to allow for any soluble impurities to be removed 7. Wash, dry and weigh the crystals within the filter paper. Observatio ns and Results Data Analysis Table 1 : Mass of rock salt Mass of rock salt / g : Table 2 : Mass of purified salt Mass of filter paper and crystals / g Mass of filter paper / g Mass of crystals / g : 1. Mass of purified crystals (g) = mass of filter paper and crystals (g) – mass of filter Princes Town West Secondary Unit 2 Chemistry Labs 75 | P a g e paper (g) 2. Percentage by mass of salt crystals recovered from the sample of rock salt = mass of purified crystals (g)mass of rock salt (g) x 100 Discussion : Conclusion : • Lab # 18 Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Analytical Chemistry Back Titration Aim : To determine the mass of 2-ethanoylhydroxy-benzoic acid in a commercial brand of an aspirin tablet by back titration Aspirin is an analgesic compound which has to ability to reduce inflammation and hence acts as both a pain killer and a fever reducer. The active ingredient in aspirin tablet is 2-ethanoylhydroxy-benzoic acid (acetylsalicylic acid), the ester linkage in aspirin is hydrolyzed/broken by alkalis to form ethanoate ions and 2hydroxybenzoate (salicylate) ions. Theory : CH COOC H COOH + 2OH3 6 4 CH3COO- + HOC6H4COO- + H2O An aspirin tablet can be hydrolyzed with excess sodium hydroxide and then back titrated with hydrochloric acid. The amount of alkali used for the hydrolysis can then be calculated and from this information, the mass of 2-ethanoylhydroxybenzoic acid in an aspirin tablet can be determined. Apparatus and Material : 1. Aspirin tablets 2. Standard 0.2 mol dm-3 NaOH 3. Standard 0.1 mol dm-3 HCl Princes Town West Secondary Unit 2 Chemistry Labs 5. 25 cm3 pipette 6. 3 conical flasks 7. Bunsen burner 76 | P a g e 4. 50 cm3 burette Procedure : 8. Phenolphthalein indicator 1. 25 cm3 of NaOH was pipetted into a conical flask and one aspirin tablet was added to this flask. The solution was then warmed gently to allow the tablet to dissolve. 2. The solution was then cooled, and three drops of Phenolphthalein indicator was then added to it. 3. This solution was then titrated with hydrochloric acid. 4. Steps 1 to 3 were then repeated until accurate results were obtained. Table 1: Volume of HCl used in the titration Rough Observation s and Results : Accurate Final burette reading / cm3 8.6 17.2 25.8 34.3 Initial burette reading / cm3 0.0 8.6 17.2 25.8 Volume of HCl used / cm3 8.6 8.6 8.6 8.5 25 cm3 of aspirin and NaOH required 8.6 cm3 of HCl in the titration. Discussion : 1. Write a balanced equation for the reaction between hydrochloric acid and sodium hydroxide 2. Calculate the number of moles of NaOH in the 25 cm3 of alkali used. 3. F ind the number of moles of HCl used in the titration 4. How many moles of NaOH reacted with HCl in the titration 5. How many moles of NaOH reacted with the 2-ethanoylhydroxy-benzoic acid in one aspirin tablet. 6. Calculate the number of moles of 2-ethanoylhydroxy-benzoic acid in one aspirin tablet. 7. Determine the mass of 2-ethanoylhydroxy-benzoic acid in one aspirin tablet. Conclusion : Princes Town West Secondary Unit 2 Chemistry Labs 77 | P a g e Princes Town West Secondary Unit 2 Chemistry Labs 78 | P a g e Lab # 19 Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Environmental Aim : To test for pollutants in water Pollutants found in water include cyanide, lead (II) ions, nitrates and phosphates. Cyanide is converted to thiocyanate when it reacts with sulphur. This ion is found in effluents from mining and industrial factories. Certain pesticides also contain thiocyanate ion as the main ingredient. Phosphates are nitrates are present in fertilizers and detergents, and these can also pollute the waterways. Theory : Carry out the following procedure to test for SCN-, NO3-, PO43- and Pb2+. SCN- gives a blood red colour on addition of aqueous iron (III) nitrate. PO43- gives a bright yellow precipitate when warmed with ammonium molybdate NO3- is identified via the brown ring test A white precipitate is formed with Pb2+ on addition of hydrochloric acid. Apparatus and Material : Procedure : 1. Samples of Polluted water P, Q and R 2. 0.1 mol dm-3 iron (III) nitrate solution 3. 0.1 mol dm-3 ammonium molybdate 4. Solid iron (II) sulphate 5. Bunsen burner 6. spatula 7. Conc. H2SO4 8. Conc. HNO3 9. Bench HCl and H2SO4 10. Test tubes 11. Water bath 1. Iron (III) Nitrate was added drop by drop to 2 cm3 of sample P in a test tube. Observations were made and recorded. Princes Town West Secondary Unit 2 Chemistry Labs 79 | P a g e 2. 2 cm3 of sample P in a test tube was acidified with dilute H2SO4. Half a spatula of solid iron (II) sulphate was then added to the acidified sample. 3. To this acidified sample of P, 0.5 cm3 of Conc. H2SO4 was then carefully added, by letting it flow down the side of the test tube. The mixture was then left undisturbed until two separate layers were formed within the test tube. Observations were made ad recorded. 4. 2 cm3 of sample P in a test tube was acidified with Conc. HNO3. A little amount of ammonium molybdate was then added and the tube was then gently warmed in a water bath. Observations were made and recorded. 5. Dilute HCl was added to 2 cm3 of sample P in a test tube. The mixture was then heated for one minute and then allowed to cool. Any observations were recorded. 6. Steps 1-5 were then repeated for both sample Q and R Table 1 : Observations made during the experiment for all three samples P, Q and R Observations with: Observatio ns and Results : Sample s Fe(NO3)3(aq) Fe2+(aq)/conc. H2SO4 (NH4)2 MoO4(a HCl (aq) q) P Q R Data : Analysis and Interpretati on 1. Deduce which of the samples of water are polluted with: Thiocyanate ions • Nitrate ions • Phosphate ions • Lead (II) ions • Princes Town West Secondary Unit 2 Chemistry Labs 80 | P a g e Justify your answers. Conclusion : Princes Town West Secondary Unit 2 Chemistry Labs 81 | P a g e Lab # NA Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Plan and Design : Organic Chemistry Aim : Organic Case Mr. Smith wants to buy a new car. He has a choice of vehicles which run on leaded gasoline, unleaded gasoline, compressed natural gas, and diesel. His : environmentally conscious friend suggests he buys a car which contributes to the least amount of pollution. Suggest which fuel is most environmentally friendly and plan and design an experiment to test your suggestion. 1. Apparatus and Material : Procedure : 2. 1. 2. 3. 4. 5. 6. Observatio ns and Results : Princes Town West Secondary Unit 2 Chemistry Labs 82 | P a g e Data Analysis and : Interpretati on Discussion : • • Conclusion : Princes Town West Secondary Unit 2 Chemistry Labs 83 | P a g e Lab # 20 Skills Assessed: Date: O/R/ R M/M A/I P/D Title : Plan and Design : Analytical Chemistry (Gravimetric) Aim : Caesium nitrate, CsNO 3, decomposes on heating. Case The decomposition is represented by one of the following equations. 4CsNO3(s) 2Cs2O(s) + 4NO 2(g) + O2(g) 2CsNO3(s) 2CsNO2(s) + O2(g) You are to devise a method of heating the solid nitrate, collecting the gas given off and measuring its volume. : From the experimental results you are to determine which is the correct equation for the decomposition. Information that may be used to prove your plan and design. The molar volume of gas, Vm, is 24.0 dm 3 mol–1 under room conditions. Nitrogen dioxide, NO 2, a toxic gas, is soluble in water. Oxygen, O2, is not soluble in water. [Ar; Cs, 133.0; N, 14.0; O, 16.0.] Princes Town West Secondary Unit 2 Chemistry Labs 84 | P a g e 1. Apparatus and Material : Procedure : 2. 1. 2. 3. 4. 5. 6. Diagram : Observatio ns and Results : Data Analysis and : Interpretati on Discussion : • • Conclusion : Princes Town West Secondary Unit 2 Chemistry Labs 85 | P a g e