RNA Quality Control — Bioanalyzer 2100 Request Form
Manager 林慶君 June Ching-Chun Lin
R401-1 / TEL#9524
User Name
Date
P.I. Name
Tel
E-mail add.
Lab number
Please fill out the questionnaires before you using Bioanalyzer2100
1. Sample Type
□total RNA (nano range)
□total RNA (pico range)
□mRNA (nano)
□mRNA (pico)
□small RNA
2. Sample Volume
5 ul (at least)
3. Sample requirement
※【NOTE】For better baseline stability, please dilute RNA samples with Nuclease-free
H2O instead of DEPC- H2O, if necessary.
x Concentration RNA 6000
Nano
total RNA
RNA 6000
Nano
mRNA
RNA 6000 Pico
total RNA
RNA 6000 Pico
mRNA
Small RNA Assay
Range
25–500 ng/uL
25–250 ng/uL
50–5000 pg/uL
250–5000 pg/uL
50-2000 pg/uL of
purified miRNA
Optimum
100–200 ng/uL
100–200 ng/uL 250–1000 pg/uL 500–2000
pg/uL
200-1000 pg/uL
4. Total sample number
No.
1
2
3
4
5
6
7
8
9
10
11
12
Sample name
Sample source
(tissue or cell line)
OD 260/280
OD 260/230
ratio (≧1.8)
ratio (≧1.8)
ng/uL