1-Find out the exon-intron structure of a gene or pre-mRNA
1A. Briefly describe how to determine the exon-intron structure of a gene (including
sizes)
Gene: TGFB1 transforming growth factor beta 1 [ Homo sapiens (human) ]
Gene length 1……………………………………………………2780
CDS
879……………………………………………….2051
5’UTR
1…………………………………………………..878
3’UTR
2052………………………………………………2780
Exons positions
1.
2.
3.
4.
1- 1233 1222 nts
1234- 1394 160 nts
1395-1512 117 nts
1513-1590 77
Introns position
1. 41348295-41353922 5627nts
2. 41344743-41348456 3713nts
3. 41342169-41344866 2697nts
FOWARD PRIMER ATGGTGGAAACCCACAACGA
REVERSE PRIMER ATGACACAGAGATCCGCAGTC
FOWARD PRIMER XXXXXATGGTGGAAACCCACAACGA
REVERSE PRIMER XXXXXATGACACAGAGATCCGCAGTC
BamHI: CTAGCXAUG
ApaI: GGGCXXAUG