• Class: MYP Year 4 – Grade 9 • Criterion C : Processing and evaluating • Unit title: Digestion • Statement of Inquiry: Energy and balance within a system can maintain an individual’s health and wellbeing. Date: ____/09/2022 Name: • Grade: 9 A / B The questions in this report will assess you on criterion C. You should attempt all the questions Refer to the rubric in page 2 to make sure you are answering correctly. • You have achieved a level: • What could you do next time to improve your achievement level? Page 1 of 9 Criterion C: Processing and evaluating Achievement level 0 Level descriptor The student does not reach a standard identified by any of the descriptors below. The student is able to: 1–2 i. collect and present data in numerical and/or visual forms ii. interpret data iii. state the validity of a hypothesis with limited reference to a scientific investigation iv. state the validity of the method with limited reference to a scientific investigation v. state limited improvements or extensions to the method. The student is able to: i. correctly collect and present data in numerical and/or visual forms ii. accurately interpret data and describe results iii. state the validity of a hypothesis based on the outcome of a scientific investigation iv. state the validity of the method based on the outcome of a scientific investigation v. state improvements or extensions to the method that would benefit the scientific investigation. 3–4 The student is able to: i. correctly collect, organize and present data in numerical and/or visual forms ii. accurately interpret data and describe results using scientific reasoning iii. outline the validity of a hypothesis based on the outcome of a scientific investigation iv. outline the validity of the method based on the outcome of a scientific investigation 5–6 outline improvements or extensions to the method that would benefit the scientific investigation. The student is able to: v. 7-8 i. correctly collect, organize, transform and present data in numerical and/ or visual forms ii. accurately interpret data and describe results using correct scientific reasoning iii. discuss the validity of a hypothesis based on the outcome of a scientific investigation iv. discuss the validity of the method based on the outcome of a scientific investigation v. describe improvements or extensions to the method that would benefit the scientific investigation. Page 2 of 9 Command Terms Reference: Command Term Definition Describe Give a detailed account or picture of a situation, event, pattern or process. Identify Provide an answer from a number of possibilities. Recognize and state briefly a distinguishing fact or feature. Verify Provide evidence that validates the result. Discuss Offer a considered and balanced review that includes a range of arguments, factors or hypotheses. Opinions or conclusions should be presented clearly and supported by appropriate evidence Determine Suggest Obtain the only possible answer Propose a solution, hypothesis or other possible answer Page 3 of 9 Topic Overview: One of the factors for the “Well-being” of individuals is to have a balanced food diet. A balanced diet provides all required raw materials for the proper building and function of our body. From energy production to immunity and cell division: all processes need nutrients provided from food. Different organic polymers are composed of different monomers connected by different types of bonds. In this session, the presence of different type of organic compounds will be tested in different types of food. “Complex Food” is a term used to describe a certain type of food that is qualitatively balanced – this means it contains many different organic compounds. Check the following table for different biochemical tests (reagents) used to identify the presence of different organic compounds. These are qualitative tests that do not give us values, however, they help in identifying content. Compound Tested (substrate) Reagent (dissolved in water = aqueous) Iodine Initial Color Positive Result Special Conditions Brown-orange Dark Blue None Reducing Sugars (Carbohydrate) Fehling-Benedict Test Blue Brick-red precipitate Requires Heat Proteins and Polypeptides Biuret Test (copper sulfate + sodium hydroxide) DCPIP Blue Violet color None Blue Colorless None Starch (Carbohydrate) Vitamin C Notes about the Vitamin C Test: Page 4 of 9 Test for Lipids (fats): As for Lipids, they are insoluble in water: this means that we can not make an aqueous solution of fat or oil on which to carry out a biochemical test → we need to perform a physical test called “emulsion test” to test for lipids. The steps to perform emulsion test are represented in the adjacent figure: Hypothesis and Aim: A scientist suggested that all food contains all types of organic material. In order to validate this hypothesis, the tests above are performed on different food groups and the results obtained will then be recorded. Procedure(s) ❖ Test for starch: 1. Place a small amount of food on the spotting tile. 2. Add a few drops of iodine solution to the food on the spotting tile. 3. Yellow–red iodine solution turns blue– black if starch is present. 4. Repeat steps 1–3 for other types of food. 5. Record all your results in a results table. ❖ Test for sugar: 1 Place a small amount of food in a test tube. 2 Add enough Benedict’s solution to cover the food. 3 Place the test tube in a warm water bath for 10 minutes. 4 Blue Benedict’s solution turns brick red on heating if a sugar such as glucose is present. 5 Repeat steps 1–4 for other types of food. 6 Record all your results in a results table. ❖ Test for protein: 1 Place a small amount of food in a test tube. 2 Add 1 cm3 of Biuret reagent. Alternatively, add 1 cm3 of sodium hydroxide solution and then add a few drops of copper sulfate solution. 3 Blue Biuret reagent turns purple if protein is present. 4 Repeat steps 1–3 for other types of food. 5 Record all your results in a results table. ❖ Test for lipids (fat): 1 Place a small amount of food into a test tube. 2 Add a few drops of ethanol to the test tube. 3 Shake the test tube and leave for one minute. 4 Pour the solution into a test tube of water. 5 Ethanol added to a solution gives a cloudy white layer if a lipid is present. 6 Repeat steps 1–5 for other types of food. 7 Record all your results in a results table. Page 5 of 9 A. Raw Data Collection: Fill in the following table with the result observed after performing each test. Food Iodine test Fehling test Biuret test DCPIP Emulsion test Grapes yellow Brick-red blue blue No change Banana Dark blue Blue blue colorless No change Potatoes Dark blue Blue Blue Blue No Change Egg White Yellow Blue Violet Blue No Change Milk Yellow Brick-red precipitate Violet Blue No Change Oil Yellow Blue Blue Blue White emulsion formed Bread Dark blue Blue Violet blue White emulsion formed B. Processed Data: Referring to the table in section A, identify the organic constituent of each food used by filling the table below. Food Positive Observation(s) Grapes Banana Potatoes Egg White Page 6 of 9 Inference(s) Milk Oil Bread C. Results Interpretation 1. Describe the process of performing Biuret Test on the piece of bread. Make sure to use the legends. __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ 2. The teacher carried out a risk assessment before she started this practical test for proteins in bread. Complete the following risk assessment for testing a food for the presence of protein. Hazard Risk Biuret Reagent Bench contamination Page 7 of 9 Control 3. “Bread is a complex food.” Verify this statement. __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ 4. “A scientist suggested that all food contains all types of organic material.” Based on the results, discuss the validity of the scientist’s hypothesis. __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ 5. Choose one of the methods used to discuss whether this method is valid and enough for proper identification of food constituents. __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ 6. Rami suffers from a disease called “diabetes”. He can’t have a lot of sugar per day. For this reason, the Dr. limited carbohydrate intake for a maximum of 130 grams per day. Now, Rami wanted to have a piece of dessert. He suggested to perform iodine test to know if he can have this dessert or not in order to maintain his well-being. 5.1 Discuss if performing Iodine test is enough for Rami to maintain his well-being by having the dessert. __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ Page 8 of 9 5.2 After doing some research, describe an improvement or an extension to the method of iodine test that would benefit Rami to make a proper decision. __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ __________________________________________________________________________________________ Page 9 of 9