Course Title: Anatomy of Vascular Plants Course Instructor: SALMAN SAEED Botany department UNIVERSITY college of management & Sciences, Khanewal, PAKSITAN 2 Definition Study of internal structure of various parts of the plant. PLANT BODY: Organs (root, stem, leaves) Tissues (ground tissues, vascular tissues, dermal tissues) Cells (ground, vascular and dermal tissue cells) Salman Saeed Lecturer (Botany) 3 Salman Saeed Lecturer (Botany) 4 The practical application of anatomical knowledge to diagnosis and treatment. • Also known as clinical anatomy. • Salman Saeed Lecturer (Botany) 5 Identification and classification Taxonomic application Medicinal plants Food adulterants and contaminants Animal feeding habit Wood present day Wood in archaeology Forensic applications Paleobotany Applied aspects of meristem culture Salman Saeed Lecturer (Botany) 6 Cytologists, geneticists, ecologists, plant breeders, chemists and anyone using plants for medicine, food, furniture, fabric or building material, or those conducting molecular research on plants, must be able to identify their source material through anatomy. Natural, accurate and reliable classification result from taking into account features of anatomy, morphology, palynology etc. Better and easier identification and classification. Salman Saeed Lecturer (Botany) 7 Anatomical data is useful in discerning evolutionary trend and relation between taxonomic categories. Example 1: • genera Anarthria and Ecdeiocolea were formerly treated as members of the Restionaceae. • An extensive anatomical survey of the family Restionaceae showed the two genera to be misfits. Example 2: • Tree leaf material in China was examined anatomically • Plant was Pycnarrhena macrocarpa. • Further study of species from this genus led to discovery of new genusEleutharrhena Salman Saeed Lecturer (Botany) 8 Group of stomata in abaxial surface of Eleutharrhena macrocarpa (a). In (b), Pycnarrhena pleniflora, the stomata are scattered over the abaxial leaf surface. Salman Saeed Lecturer (Botany) 9 Drugs are extracted from leaves, barks, roots or rhizomes of plants. It is quicker to find out the identity of crude drugs from its anatomy than its chemistry. Pure material and adulterants are differentiated by anatomical confirmation. Example: • Ipecacuanha, used in cough mixture can be adulterated with roots from alternative inferior species. • The authentic source of the drug is Cephaelis ipecacuanha (Rubiaceae). Salman Saeed Lecturer (Botany) 10 • • • Salman Saeed Lecturer (Botany) Ipecacuanha, used in cough mixture can be adulterated with roots from alternative inferior species. The authentic source of the drug is Cephaelis ipecacuanha (Rubiaceae). Sometimes Cephaelis acuminata is used as a substitute. 11 • • • • • Occasionally substitute may be poor or unsuitable. Rheum officinale root and rhizome is used medicinally. But Rheum rhaponticum is the vegetable. Fortunately, chemical and anatomical tests can be applied to detect which species is present. Digitalis purpurea and D. lanata are used medicinally. They can be distinguished from one another on anatomical grounds Salman Saeed Lecturer (Botany) 12 Salman Saeed Lecturer (Botany) 13 Salman Saeed Lecturer (Botany) 14 Some herbs are used extensively in seasoning. These are often imported in the form of dried powdered plant parts. It would be easy to introduce useless or sometimes even poisonous adulterants which would be difficult to detect with the naked eye. Ailanthus leaf has also been used as a mint adulterant. Salman Saeed Lecturer (Botany) 15 Foreign bodies sometimes get into food by accident. Often these are small and fragmentary and can be identified only with the microscope. Example: A splinter of wood in butter was found to come from a species of Pinus. The importer and packers hoped to be able to determine if the splinter could have come from the country of origin of the butter, or whether it might have been introduced during the packing stages. Salman Saeed Lecturer (Botany) 16 Animal sometimes consume crop plants. It is possible to find out what has been eaten by studying the composition of faeces, or stomach. For example faeces from rabbits, foxes etc. Fragments of plant are very small when they have passed through an animal’s digestive system. Fragments are put into a petri dish, and the sample divided into its components. These fragments from each dish are examined using under the light microscope. Some African cattle were being injured by eating grasses. The cattle only ate the grass when other plants were unavailable. Faeces were examined and reported that there were silica bodies and sharp hairs present. Domestic animals occasionally eat poisonous The owner of the animals can then take precautionary measures against further livestock poisoning. Salman Saeed Lecturer (Botany) 17 samples sent to Kew for anatomical identification consist mainly of wood. The samples derived from different sources. The British Standards Institute has published a list of common names and the species from which the woods come, and this is the authoritative work which has to be followed. Preservation of wood is considerable economic importance. experimental anatomy carried out in various parts of the world in order to establish prevention of their degrading activities. Salman Saeed Lecturer (Botany) 18 These characters also enable to identify the wood and charcoal preserved in sites from antiquity. Features like perforation plate and lateral wall pitting are still retained. The wood of archaeological sample is compared with that of present-day-wood India produce evidenceuse of wood. The Indus Bronze Age civilization of Harappa and the Copper Age civilization of Hastinapura, were aware by uses of wood. Deodara for making coffins has property of shock absorbing and Harappans were aware of these fact. Dalbergia sissoo and Holarrhena antidy senterica these two timber-yielding plants. These plants provide fuel woods. Corylus were well preserved in waterlogged condition in Somerset at Bronze Age. Apart from wood and charcoal other archaeological plant remains are also preserved. For example a sandal was preserved in ancient Egypt. Salman Saeed Lecturer (Botany) 19 The application of forensic science is helpful in investigating a crime. Use of plant materials in solving crimes or other legal problems. The morphological and anatomical diversity expressed by plant species provide characters to identify plant parts. Plants remains are present every where in form of macroscopic pieces and microscopic forms. Edible plants are used to identify the stomach contents and last meal of a victim. In dicotyledonous roots growth rings can indicate the number of years passed since burial. Dendrochronology techniques enable to date the wood and so the year of painting. These techniques are also used to detect when the wood was used to make art objects or musical instruments etc. Salman Saeed Lecturer (Botany) 20 EXAMPLE: • In 1932, in the evening of March 1st the infant son of famous American aviation hero Charles Lindbergh was kidnapped from his home in Hopewell, New Jersey, US. • dead body of the son was discovered a few miles away from the family home. • The ladders were the only evidence left at the scene. They were homemade and crude. • Xylotomist Arthur Koehler of United state Forest Services in Wisconsin examined the wood of the ladder both morphologically and anatomically. • four species were used to construct the ladder —namely Yellow pine, Pinus, ponderosa, Douglas fir and Betula sp. • The basis of identification was the microscopic analysis of grain patterns of the woods. Salman Saeed Lecturer (Botany) 21 - Helps in identification of fossils Placed in extant families and genera Determines if roots or stem Fossils may be sectioned, grounded Salman Saeed Lecturer (Botany) 22 Apical Meristem At tips of root, shoot and leaf The shoot is used in culture by cutting it out and placing in nutrient medium (composition and conditions) Micropropagation Usage in horticulture, forestry, agriculture Conservation of endangered species (orchids) Example: Manihot esculentus Salman Saeed Lecturer (Botany) 23 Meristem at internodes Adventitious roots formed at nodes Horticulture use – propagation of plants by stem cuttings Example: Dianthus Salman Saeed Lecturer (Botany) 24 On lateral sides, consisting of vascular and cork cambium Example: Cork cambium and fascicular cambium Wound can regenerate Employed in plant propagation by cutting and grafting Salman Saeed Lecturer (Botany) 25 Identification – in giving correct names Identification of medicinal plants Classification – in classifying correct families and orders For taxonomists Paleobotany – fossils classification Improving food quality Meristem culture – in propagation and removing diseased plants Quality of furniture improved by identifying wood Salman Saeed Lecturer (Botany) 26 Salman Saeed Lecturer (Botany) Salmanbotanist@gmail.com Salman Saeed Lecturer (Botany) 27