Name: _________________ Period: ______ Partner__________________ Cellular Respiration Activity Problem: How does exercise affect the amount of disposal of waste (CO2) from cellular respiration? Hypothesis: I predict increased exercise will _____________________my amount of carbon dioxide production because _______________________________________________________________ Materials: 3 small test tubes 10 ml graduated cylinder test tube rack bromothymol blue solution 2 straws clock or stopwatch goggles Procedure: 1. Record your hypothesis, as to how exercise will affect your body’s production of CO2 2. Label test tubes A, B, and C. Put 10 ml of water and a few drops of bromothymol blue in each test tube. (Bromothymol blue will turn from blue to yellow or green in the presence of carbon dioxide) 3. When your partner says ‘go’, gently blow air through the straw into the bottom of test tube B. CAUTION: Do not inhale through the straw! 4. Your partner will record the time it takes for the color to change. 5. Switch roles and repeat for the second partner. 6. Now jog in place for 1 minute and repeat steps 3 through 5 using test tube C. CAUTION: If you feel dizzy or weak while exercising, stop immediately and sit down. 7. When you are finished with your activity, give your results to your teacher. Data Collection: Time for solution to change color(sec) for ME Time for solution to change color(sec) for my partner Class Average Time (sec) Before exercise After exercise Data Analysis: 1. Why did we have test tube A?___________________________________________________ 2. What was the Independent Variable? _____________________________________________ 3. What was the Dependent Variable?_______________________________________________ 4. What process in your body produces carbon dioxide? _______________________________ 5. What happens to the carbon dioxide you produce in your cells? ________________________ 6. How does exercise affect this process? ___________________________________________ Conclusion: 1. Address your hypothesis: How did exercise affect the time for the solution to change color? _____________________ ___________________________________________________________________________ Did these results support/reject your hypothesis? ____________________________________ ___________________________________________________________________________ 2. Error analysis – what could be 2 sources of error in this lab? ________________________________________________________________________________ ______________________________________________________________________