Structure and classification of microbes Structure and classification of microbes Virus: Although they may seem to behave as living things,viruses are actually acellular, nonliving particles. Viruses are not considered to be living organisms because they are incapable of carrying out all life processes. – Prokaryotes have a relatively simple morphology and lack a true membrane-bound nucleus -----Bacteria – Eukaryotes are morphologically complex and have a true, membrane-bound nucleus ----- Fungi, Protozoa, algae – Prokaryotes • have a relatively simple morphology and lack a true membrane-bound nucleus -----Bacteria – Eukaryotes • are morphologically complex and have a true, membrane-bound nucleus ----- Fungi, Protozoa, algae Prokaryotic Cells Nuclear region (nucleoid) is not enveloped by a nuclear membrane. Eukaryotic Cells Nucleus is surrounded by a double membrane layer. Single chromosome present (circular) More than one chromosome are present (linear) True nucleolus is absent. Nucleolus is present. An area inside the nucleus of a cell that is made up of RNA and proteins and is where ribosomes are made Membrane bound organelles are absent. Membrane bound organelles are present. Endoplasmic reticulum, Golgi apparatus, lysosomes, Mitochondria Prokaryotic Cells Eukaryotic Cells Cell size is 1-10μm They are comparatively larger in size. Cell size 10 - 100µm. 70 S ribosome (50+30) 80 S ribosome (60+40) Multiplication of cell is by fission or budding. Cell division by mitosis or meiosis. Cell Walls preset, which are chemically complex. Cell walls seen in only plant cells, which are chemically simpler. Cell type is usually unicellular. Usually multicellular cells. Example: Bacteria, Example: Yeat, Protozoa, animal cells and plant cells. They are very minute in size Size & Shape of bacteria • The size of bacteria is measured in micrometer (m) or micron () • 1 m=0.001mm = 10−6 m • Most pathogenic bacteria measure from 0.1 to 10 . Morphological Classification of Bacteria Shape of Bacterial Cell The three basic bacterial shapes are coccus (spherical), bacillus (rod-shaped), and spiral (twisted), however pleomorphic bacteria can assume several shapes. Cocci • From kokkos meaning berry • coccus for a single cell Coccus 1. Diplococci- in pair Streptococcus pneumoniae (pneumococci) Neisseria gonorrhoeae (gonococci) Neisseria meningitidis (Meningococci ) Diplococcus 2. Streptococci- in chains Streptococcus pyogenes Streptococcus 3. Tetracocci- 4 Aerococcus, Pediococcus Tetrad 4. Sarcine- 8,16,32.. Sarcina ventriculi Sarcinae 5. Staphylococci- cluster Staphylococcus aureus Staphylococcus • Diplococci— Streptococcus pneumoniae (pneumococci) Neisseria gonorrhoeae (gonococci) Neisseria meningitidis (Meningococci ) • Streptococci– Streptococcus pyogenes • Tetrads-Aerococcus, Pediococcus • Sarcinae Sarcina ventriculi • Staphylococci Staphylococcus aureus Arrangement of Bacilli • From baculus – meaning rod Klebsiella pneumoniae B.subtilis corynebacterium diphtheriae e. Coccobacilli g. Vibrio They are comma-shaped bacteria with less than one complete turn or twist in the cell Haemophilus influenzae f. Trichomes: Vibrio cholerae Vibrio cholerae saprospira grandis The bacilli are arranged in chains with larger area of end-to-end contact between the cells. Arrangement of Spiral Bacteria • Spirilla rigid spiral structure Campylobacter jejuni, Helicobacter pylori • Spirochetes flexible spiral structure Leptospira interrogans, Treponema pallidum Others Shapes and Arrangements of Bacteria • Filamentous bacteria – Actinomycetes --Resemblance to radiating rays of sun --Some of them form branching filaments resulting in a network of filaments called ‘mycelium’. Candidatus savagella • Mycoplasmas >---do not have cell wall & hence do not posses fixed shape Pleomorphic Bacteria Mycoplasma pneumoniae, M. genitalium