Which of the following components is not involved during the formation of the replication fork?
c. DNA ligase
Which of the following enzymes is the principal replication enzyme in E. coli?
b. DNA polymerase III
Overwinding or over tightening of DNA during replication is caused and removed by
d. DNAb, gyrase
Which translation factor facilitates release of peptide and ribosome?
c. RF3
Which of the following is true about DNA polymerase?
Select one:
a. It can synthesize DNA in the 5’ to 3’ direction
Human cells were infected with the virus carrying the genes for telomerase. What could be the
result of such modification?
c. the cells will undergo more cell divisions than normal
You recognised that due to the incorrect nucleotide incorporation the in vitro transcription was
interrupted due to the stalled RNA polymerase. Which factor could be used to overcome this
problem?
c. TFIID
Which of the following proteins is able to reverse the Central dogma of molecular biology?
Select one:
a. Reverse transcriptase
Choose the snRNP’s that form the triple complex during the splicing.
c. U4, U5, U6
Which snRNP recognises 5’ splice site and facilitates in lariat formation?
Select one:
a. U6
What is the function of helicase?
b. It separates DNA strands
Which general transcription factor is capable of phosphorylating RNA pol II CTD on its serine
residues?
Select one:
a. TFIIH
Choose the termination method for prokaryotes
1. Release factor binding
2. Rho-dependent termination
3. Hairpin loop (stem loop) formation
Select one:
a. Only 2 and 3
Choose which of the following proteins doesn’t participate in recombination?
b. DnaF
Okazaki fragments occur on the __________ and are bonded together by __________
Select one:
d. Lagging strand, ligase
Which of the following do not belong to promoter elements?
d. TATA box
The study was conducted on the importance of various DNA polymerases and they were
mutated one by one. Choose which out of the following will be associated with the least survival?
Select one:
a. DNA Pol delta
Which feature best describes rho protein’s termination action?
Select one:
a. Recognition of A:T reach sequence
b. Helicase activity
Choose out of the following the mechanism which is used for repair lesions in DNA induced by
chemical modification.
Select one:
a. Mismatch repair
During the transcription there is a cleavage of pyrophosphate from incoming __________
Select one:
a. Ribonucleoside triphosphate
In homologous genetic recombination, RecA protein is involved in:
Select one:
a. involved in pairing homologous DNA and strand invasion
Choose the incorrect statement about the post-transcriptional modification of tRNA.
b. Polyadenylation
Which one of the following proteins functions to significantly increase the processivity of DNA
Polymerase III?
e. The β-clamp (or sliding clamp)
What is required for recognition of promoter region in prokaryotes?
d. Sigma factor
During the homologous recombination DNA cleavage enzyme generates ___________ on the
site of double strand breaks.
Select one:
a. Single stranded DNA
Choose the correct statement about the mammalian DNA repair systems
c. They are able to repair more than 99% of the DNA lesions
Choose the correct protein set that migrates the holliday junction in homologous recombination.
b. RuvAB
Which following protein doesn’t helicase or nuclease activity?
c. RecA
Which general transcription factor is capable of phosphorylating RNA pol II CTD on its serine
residues?
c. TFIIH
What is the first event in formation of the closed complex during bacterial transcription?
d. Binding sigma factor of RNA polymerase to -10 and -35 promoter regions
Translocation is the process whereby the __________ moves in order to place the tRNA bound
to the growing polypeptide chain in the __________ site, thereby freeing the __________ site for
a new aminoacyl-tRNA.
ribosome; P; A
In an experiment you take a DNA in vitro and attempt to replicate it. Which combination will you
add to your DNA to get the maximum replication product?
b. Dna a, Dna b, Dna c, HU ,SSB polymerase I
A biochemist isolated and purified molecules needed for DNA replication. When some DNA was
added replication occurred, but the DNA molecules formed were defective. Each consisted of a
normal DNA strand paired with segments of DNA a few hundred nucleotides long. Which of the
following had been left out of the mixture?
Select one:
a. DNA ligase
Which snRNP is able to recognise the 3’ splice site?
d. U2
Which of the following factors are necessary in translation initiation in eukaryotes?
Select one:
a. Initiation factor 2
Which of the following is not characteristic of genetic code?
d. Ambiguous
Choose which best describes the function of DNA Polymerase alpha?
Select one:
a. Lagging strand synthesis
A scientist is replicating human DNA in a test tube and has added intact DNA, the replisome
complex, and the four deoxyribonucleoside triphosphates. To the surprise of the scientist, there
was no DNA synthesized, as determined by the incorporation of radio-labeled precursors into
acid-precipitable material. The scientist’s failure to synthesize DNA is most likely due to a lack of
which of the following in his reaction mixture?
c. Dideoxynucleoside triphosphates
Eukaryotic and prokaryotes different with all mentioned statements except _______________
Select one:
a. Ability to form replication fork
Which out of the following proteins participating in homologous recombination moves along the
dsDNA unwinding the strands ahead of it and degrading them:
d. RecBCD enzyme
Which is the first enzyme that acts in the base-excision repair?
b. DNA glycosylase
Which enzyme removes the RNA primer during replication?
b. DNA polymerase I
Human cells were infected with the virus carrying the genes for telomerase. What could be the
result of such modification?
b. the cells will undergo more cell divisions than normal
Okazaki fragments occur on the __________ and are bonded together by __________
d. Lagging strand, ligase
At which end are the new DNA bases added?
c. 3’ OH end
Which of the following is not characteristic of genetic code?
Select one:
a. Ambiguous
Which out of the following isn’t necessary for rho-dependent termination?
Select one:
c. Stem loop structure
Which translation factor binds to aminoacyl-tRNA?
Select one:
c. EF-Tu
Choose which of the following proteins doesn’t participate in recombination?
Select one:
c. DnaF
n which direction does the elongation of transcript is done by RNA polymerase?
Select one:
c. 3’->5’ direction on 5’->3’ strand
If the sequence of an RNA molecule is 5’-GGCAU-3’, what is the sequence of the nontemplate
strand of DNA?
b. 3’-GGCTA-5’
Which out of the following proteins assemble into long, helical filaments?
Select one:
d. RecBCD enzyme
Aminoacyl-tRNA synthetases ________ link ________ to their respective tRNA molecules
d. covalently; amino acids
Which out of the following prokaryotic proteins or their homolog doesn’t participate in
eukaryotic replication?
Beta clamp
Which spliceosome component does SR protein recruit?
Select one:
a. U6
Where does the catalytic unit of RNA polymerase be placed during the proper initiation of
transcription?
Select one:
d. -10 site
What does the Spo11 protein do during the recombination?
Select one:
a. Making the nick
Choose the incorrect statement about mRNA.
d. Introns are removed and exons are spliced together
Choose the correct statement about the methyl directed repair system: When bacterial DNA
replication introduces a mismatch in a double-stranded DNA, the methyl-directed repair system:
d. Corrects the mismatch in the daughter strand
Which is the largest class of introns found in mRNA primary transcript?
b. Spliceosomal introns
Choose the incorrect statement about the TBP.
b. Is just associated with RNAP II