Presentation on thesis proposal
Title: chemical and biological investigation of
Eichhornia crassipes
Introduction
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Commonly known as water hyacinth
Free-floating perennial aquatic plant
Native to tropical and sub-tropical South America
Obligate plant
Thrives in all types of freshwater sources
Objectives
General
• Observe the pharmacological activity of the Eichhornia crassipes
Specific
• To know the ayurvedic uses
• To show phytoconstituents from extract
• To know antioxidant property
• To know anti-inflammatory property
• To know antiobesity property
• To know the toxicity study
Characteristics
• Spongy, glossy, leathery and waxy leaves
• Funnel shaped flowers in purple and blue with yellow spots
• Purplish to black, feathery and submerged roots
• 95% water, has a fibrous tissue
• Is similar to the native frogs bit, Limnobium spongia
• Double its population in as little as 12 days
Continued….
• Reproduction includes:
-Sexual-seeds
-Asexual-over wintering stems and the daughter plants
Habitat and ecology
• Tropical desert, sub-tropical or warm temperature desert and rain
forest zone
Taxonomic classification
Kingdom: Plantae
Division: Magnoliophyte
Class: Liliopsida
Order: Liliales
Family: Pontederiaceae
Genus: Eichhornia
Species: Eichhornia crassipes
Methodology
• Materials
Leaves of Eichhornia crassipes
Distilled water
Mice
Ethanol solution
Nose mask and hand gloves
Refrigerator
Spirit burner
Continued….
• Collection and identification of plant:
plant will be collected from any reservoir
• Extraction preparation:
Eichhornia crassipes will be collected
cut into small pieces
Continued……
will be dried into sunlight
will be grinded into fine powder
will be extracted with organic solvents (i.e. hexane, methanol)
Stored in tight container for further analysis
Continued……
• Phytochemical investigation:
-quanlitative and quantitative analysis
-separation will be done by using chromatographic methods
• Biological investigation:
-antioxidant activity (by DPPH method)
-antimicrobial activity (by Muller-Hinton agar plate disc technique)
Continued…..
-cytotoxic activity ( by brine shrimp lethality bioassay)
-anti-inflammatory activity (by well established method)
-anti-obesity activity
Literature review
Literature review