1009DOH / 1019DOH / 1040DOH Oral Biology 2 / Applied Oral Biology 2 / Clinical Dental Practice 1 Laboratory Manual Anatomy of the Head and Neck Region Laboratory 1 Embryology Learning objectives: 1- Describe the embryology of the face 2- Describe the derivatives of pharyngeal arches Embryology of the face and pharyngeal arches (Textbook of Head &Neck Anatomy, 4th Edition, Wolters Kluwer Lippincott Williams & Wilkins Philadelphia, PA, USA Chapter 5;). Pharyngeal arch embryology: Pharyngeal Arches are blocks or bars of condensed mesenchymal tissue that are located between the pharyngeal pouches and pharyngeal clefts (or grooves). Each arch is numbered in order of their location (reflecting developmental sequence), arch 1 (Mandibular), 1 (Hyoid), arch 3, arch 4 and arch 6. Arch 5 doesn’t develop in humans and although rudimentary arch 6 contributes with arch 4 to form thyroid and laryngeal cartilages and other muscular and neural structures. View timepoints 1:45 minutes to 5:30 minutes of the following video to review your understanding of pharyngeal arch development Why is learning about pharyngeal arch development of assistance for your future clinical practice? ___________________________________________________________________________ ___________________________________________________________________________ Before or after your laboratory class, review Table 5.1 page 55, Chapter 5, Hiatt J.L.and Gartner L.P. Textbook of Head &Neck Anatomy, 4th Edition, Wolters Kluwer Lippincott Williams & Wilkins Philadelphia, PA, USA Chapter 5 which describes Pharyngeal arch derivatives and their innervation. Complete your own table to summarise key information from your lectures on the pharyngeal arches and their derivatives (see sheet at end of these laboratory notes). Pharyngeal Arch 1 and embryology of the face View timepoints 5:30 minutes to 12:30 minutes of the following video to review your understanding of facial embryology http://www.youtube.com/watch?v=SG3do_BeB0M Now view this short summary animation which demonstrates the growth and fusion of the regions of the frontonasal prominence and Pharyngeal Arch 1 prominences. Please note the colours in the video represent the following: Frontonasal Prominence central portion (white) Frontonasal Prominence - Lateral nasal (purple) Frontonasal Prominence - Medial nasal (green) Pharyngeal Arch 1 - Maxillary prominence (yellow) Pharyngeal Arch 1 - Mandibular prominence (orange) http://php.med.unsw.edu.au/embryology/images/d/d7/Face_001.mov These lecture and practical notes from the University of NSW will assist your study so work your way through them. http://php.med.unsw.edu.au/embryology/index.php?title=BGD_Lecture__Face_and_Ear_Development http://php.med.unsw.edu.au/embryology/index.php?title=BGDB_Practical__Face_and_Ear_Development Sutures, sinuses and foetal skulls In adults, the cranial bones are united by fibrous joints which are synarthrotic as they do not permit movement. Note the joints between the skull bones. Locate and examine these cranial sutures: Sagittal suture: Which bones does it connect? ___________________________________________________________________________ Coronal suture: Which bones does it connect? ___________________________________________________________________________ Lambdoid suture: Which bones does it connect? ___________________________________________________________________________ Squamous suture: Which bones does it connect? ___________________________________________________________________________ What type of tissue is found in these joints? ___________________________________________________________________________ What may happen to these joints as adults age? ___________________________________________________________________________ The Pterion is a landmark feature of the skull, where the skull thickness is minimal. Which four bones connect here? 1._________________________________________________________________________ _ 2._________________________________________________________________________ _ 3._________________________________________________________________________ _ 4._________________________________________________________________________ _ Paranasal Sinuses: Clustered around the nose are mucosa-lined, air filled sinuses which are located in 5 bones of the skull (Ethmoid, Sphenoid, Frontal and each of the pair of maxillary bones). Identify two general functions of sinuses 1._________________________________________________________________________ _ 2._________________________________________________________________________ _ Where do sinus drain? ___________________________________________________________________________ Using the x-rays and the skulls, identify the air sinuses in the skull. Frontal Maxillary: NOTE the proximity of the upper molar teeth to this sinus Ethmoid Sphenoid From you observations of the location of these sinuses, briefly explain may infections spread to these paranasal sinuses. ___________________________________________________________________________ ___________________________________________________________________________ During extraction of upper molar and premolar teeth, an oroantral communication may occur. What do you think is meant by the term oroantral communication ___________________________________________________________________________ ___________________________________________________________________________ The mastoid process of the temporal bone also contains air cells connected to atmosphere via the tympanic cavity and pharyngotympanic (Eustachian) tube. What is the clinical relevance of these air cells? ___________________________________________________________________________ ___________________________________________________________________________ Foetal skull: Identify the following structures on the foetal skull model Locate the Anterior fontanelle, posterior fontanelle and mastoid (posterolateral) fontanelle and label them on the diagrams below. In the lateral view, note the relative size of the cranial vault compared to the size of the face. The mandible is particularly underdeveloped and the acoustic meatus is very shallow. See Figures 1.3 and 1.4 page 3, Head and Neck Anatomy for Dental Medicine (2010) Eric W. Baker Editor Thieme Medical Publishers Inc NY USA Or Figure 7.39a 244 Chapter 7 Marieb & Hoehn, Human Anatomy and Physiology, 2013, 9th Edition Pearson Education for foetal adult comparisons Figure 7.36ab, page 244 Chapter 7 Marieb & Hoehn, Human Anatomy and Physiology, 2013, 9 th Edition Pearson Education Now locate and name the sutures you identified in the adult skull. What difference do you notice between the sutures of the foetal and adult skulls? ___________________________________________________________________________ ___________________________________________________________________________ What type of tissue covers the fontanelles in infants? What type of ossification occurs here? ___________________________________________________________________________