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Medical Laboratory Science Comprehensive Exams Ultimate
Compilation
Medical Technology (Our Lady of Fatima University)
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CLINICAL CHEMISTRY
Coupled enzymatic rgt except
T4 in RID
Lithium in blood serum
Renal threshold
CC – 2nd take
GGT
Metabolite increase in liver dse
Starch to simple saccharide
Enzyme catalyze from starch to glucose
and maltose
Cortisol diurnal characteristic
Middle value
Tungsten blue
Most methods of tAG determination
measure ____________:
Reyes syndrome
Colorimetric bilirubin method
Uncompemsated acidosis
Bilirubin bile canaliculi
Protein not usually present in blood
Tube for glycated Hb
Conversion of urea nitrogen to urea
VLDL
SGOT
Bitter almond
Does not need fasting
Glucose level of DM
Chloride mercuric nitrite producing blue
color
Zero order
Etched pipette
Ascorbic acid inhibits w/c glucose pathway
monochromator
AAS
No included enzymatic test in in
determining glucose
Radiolabeled T4 binding with Anti T4
AES/flame
When there’s an excess the substance is no
longer reabsorb so it goes out and appear
in the urine
Liver
Ammonia
Amylase
Amylase
8-10 peak morning 11-3 evening low
Median
Uric acid in Caraway method. Also in
measuring urinary proteins under the
Folin-Ciocalteau method.
Glycerol
Ammonia
Low pH increase Carbonic acid
Dubin Johnson
Alpha 1 anti-globulin
EDTA – normally used for Hemoglobin
measurements
Multiply by 2.14
Major carrier of ENDOGENOUS
triglycerides.
Reitman Frankel
Cyanide
HbA1C
>126ug/dl
Schales schales
Excess substrate / no excess enzyme
Blow out pipette
Oxidase-reduction
Selects the band of the light that passes to
the cuvette
calcium
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Interference in bilirubin test @ 640nm
Dilution 1:5
Scattered light
precision
Icteric index
Monitor quality control between labs
(inter-lab)
Bilirubin excretion deficiency
Chelates calcium
Cholesterol reference method
% glucose in PB sa CSF
Major fraction organic iodine in circulation
drug
monochromator
Turbidity in serum
Anything that is infecting the integrity of
the serum. (Hemolysis ganyan – dapat clear
yung serum na yellow color)
4ml H2O
Nephelometry
The lower the Standard Deviation the
higher the precision
Bilirubin since Icterus is increase in
Bilirubin
Youden plot
Dubin Johnson
EDTA
Abell-kendall (mataba si Abell)
60%
Thyroxine T4? (Confirm Clin. Chem
module)
GC/MS
Disposes polychromatic light into its
separate wavelengths
chylomicrons
CLINICAL CHEMISTRY
Creatine Kinase(CK) for Acute Myocardial CK-MB and CK MM – Reference is
Infarction
Bishop’s p 272. Confirm with CC2 module
Blue color (urea, nitrogen, ammonia, uric Uric Acid – (Tungsten blue)
acid)
Cleaning glassware EXCEPT:
Cr2H2O/H2SO4
Cytoplasmic protein transport??
Ligandin and Z protein
Hexokinase
NADPH
Specific for liver
ALT
Inversely related to CHD
Apo A & HDL
Incorrect use of pipette
Random error
Gibson Cooke test? Which is true?
Refer to pg 105 CC2 (Testing for Cystic
Fibrosis, sweat test. Stimulate with
pilocarpine.)
Reproducibility
Precision
+2SD
95.5%
Steps for cholesterol
Saponification, extraction ,precipitation,
coulorometric (SEPC)
Benzoylecgonine
Cocaine,
100% T = Optical density %
0.000
Primary minerals in bone
Calcium & phosphate
Respiratory acidosis
Excess carbonic acid leading to
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decreased pH. To compensate for this
kidneys will increase amount of
bicarbonate.
Substrate of prostatic ACP
Thymolphalein
monophosphate…
(something like that)
Several test in CC required the px to for cholesterol
fasting (Fasting blood sugar, Chole, Trigly,
inorg.phosphate?)
Conjugated bilirubin characteristics
Water and alcohol soluble, renal
excretion, direct conjugated,
“Red man syndrome”
Vancomycin
YUNG SA CHARTS
AOTA
Do not move in pre-beta...
Chylomicrons
Split in albumin
Bisalbuminemia
Albumin Ref range
3.5-5.0 g/dl
Heroin synthesis from
morphine
Quantitation of TAG
glycerol
Test for neuromuscular tetany
Low calcium / hypocalcemia
Ref. Range of plasma chole.
200 mg/dl
Csf multiple sclerosis characteristics
Polyclonal banding
Test for decrease T4
TSH
Sweat chloride is for
Cystic fibrosis (Gibson Cooke Method)
Random errors
Dirty glasswares, wrong pipets, voltage,
fluctation, sampling errors
Hypophyseal structure receive signals
Hypothylamoneurophyseal tract?
Catalyzes the conjugation of bilirubin
Glucoronly transferase
ISE are associated EXCEPT:
None of the above?
Transport protein of bilirubin in blood
albumin
Ion increase in hemolysis
K because it is major intracellular anion.
Cellulose acetate principle
Size of molecule
Lipoprotein increased
Enzyme that converts starch to glucose and Amylase
maltose
Serotonin source
Tryptophan
Protein fraction rarely increased in plasma Alpha 1 antiglobulin
Kidney tubular reabsorption
Osmolarity
Hepatoma marker rarely increased in adult Alpha-1-fetoprotein
serum
Evelyn malloy
Pink (Bilirubin)
Ion selective electrode electrochemistry Potentiometry
principle
Diacetyl monoxime use
Blood UREA nitrogen
Most anodic lipoprotein
HDL
Major intracellular anion
Phosphate
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600mg/dl ; 1:300 final conc.
Flame photometry is based on
Reference Method of Cholesterol Analysis
2mg/dL
Light emitted by excited atoms
Abell-Kendall method
October Batch 1st take CC compre
1. Specimen used for G6PD
Red cell hemolysate
deficiency
2. Method for titer
Serial Dilution method
3. Molarity of 3.5 N is H2SO4
3.5/2 – 1.75
4. Relationship of SD with
SD Precision
Precision
5. Incorrect calibration of
Systematic Error
pipette causes:
6. 150 mg/dl, 1:20, 1part and 4
150 x 1/20 x 1/5 = 1.5
parts
7. State where enzyme is
a. Free unbound b. Protein -bound
metabolically active
a. Testosterone
8. Principle androgen formed by
b. Progesterone
adrenal cortex and is the least
c. Estrogen
potent among major
d. DHEA
androgens
e. androstenedione
9. Most in maternal urine
Estriol*
10. 80% in children with HVA
Neuroblastoma
11. Making 0.08mol/L of HCl
5000mL
1000mL of 0.4moL should be
diluted to what
12. In fluorescence spectro,
which is position at right
Secondary Monochromator and Detector
angle relative to the light
13. Best for protein
Nephelometry
determination
Diazo reagent - Bilirubin pigments in serum
or plasma are reacted with a diazo reagent
(sulfanilic acid in hydrochloric acid and
14. Color rgt for Jendrassik Groff
sodium nitrite), resulting in the production of
the purple product azobilirubin.
15. 4th number in enzyme
1stcategory 2nd subclass 3rd subsubclass 4th
classification
serial number
C-reactive protein during inflammation
16. Protein for RH and SLE
(Reference is Bishops) ANA?
17. AST and ALT half l ife
AST(cyto)17Hr AST(mito) 87Hr
difference
ALT(cyto)47Hr
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18. Normal in uncompensated
metabolic acidosis
19. Reduced florescence due to
interaction of molecules
20. Waste product of muscle
dystrophy
21. BUN is more sensitive that
Creatinine
22. Jaffe reaction Rgt
23. What’s detected in Reye’s
Syndrome
24. Caraway method color
reaction
25. Generates energy except
26. Produced by cells in the
Jejunum and locer duodenum
in response to low pH
27. Insulation to vital organs
28. The size of lipoporteins
correlate with its lipid
content
The larger the size the lighter in
density
29. Most anodic form
30. Increased of this would
decrease CHD risk
31. 2mL milky plasma-refigerate
overnight:
32. +float –tubbidity
33. Yellow color in Hazard
Symbol
34. Best way to break chain of
infection
35. Glass, needle/lancets are
disposed where?
36. 2 consecutive controls fall
outside 2SD
37. Used to check accuracy and
Precision
38. Coefficient of variation
pH….pCO2….pO2…HCO3
quenching
Creatine, creatinine
True or false
Picric acid in alkaline (Alkaline picrate)
Ammonia
Purple….Red-orange…BLUE…Yellow (based
on the oxidation of uric acid in a protein-free
filtrate, with subsequent reduction of
phosphotungstic acid in alkaline solution to
tungsten blue.)
EMP…Hexokinase…GLYCOGENESIS…Pentose
phosphate shunt
Secretin
TAG, Cholesterol Ester
Both are TRUE
HDL LDL vLDL Chylomicrons
Apo A or HDL
Chylomicrons only
Y-reactivity B-Health R-Fire
Handwashing
Puncture proof container
22s
Control?
SD/Mean (100)
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39. Measure of closeness of
repeated analysis
40. 2SD=2.0-2.6 expected that
one result will fall greater
than 2.6 is 1 in
41. What to do after repeating the
control after it has been out of
control?
42. Variants except:
43. CSF total Protein
concentration
Precision
100, 10, 20, 40
Run new control from different lot
Check for systemic error
Run new control from same lot but new
vial
Nagao, kasahara, regan, osteoblastic
15-45 mg/dL
Na+ major extra cation
K+ major intra cation
Bicarb major intra anion, (only 2nd)
Cl+ major extra anion
44. Which is wrong
45. Primary compound of thyroid
compound synthesis
46. Flipped LD 1 and LD2
indicates, increased CK
47. Increased in Liver disease
except
48. Earliest to increase in AMI
49. Hydrolases except
50. In AST and ALT, which is not
true
51. Role of PTH in urine water
balance
52. Increased by 3-10% in serum
when left standing at 25 or
4’C
53. Only hormones secreted by
posterior pituitary
54. Besides insulin, Main control
for blood glucose
55. Type of DM in cow’s milk
56. OGTT with the highest conc of
Glucose
57. Renal Glucose Threshold
58. Major lipid found in VLDL
59. Proper PPE in routine chem
Lab
Iodine
Myocardial infarction
ALD…ALT…ALP…pCHE
Troponin1…CK-MB…Troponin2…Myoglobin
ACP, ALP, 5N, AChE, ALT, SChE, LPS, AMS,
AST is intra and extra ALT only intra
AST is more spec that ALT
Both
D. Neither
Ca+ reabsorption
Inhibits K+ for Na+ reabsorption
Inhibits Cl+
ALD…ALT…ALP…AST
Vasopressin(ADH) and oxytocin
Glucagon CC! module p.76
DM1
1 Hr after meal peak reference: cc1 p.83
160-180 mg/dL
TAG
Lab gown, gloves, mask
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60. In Primary hyperthyroidism
TSH is…
61. Hormone asso with
Amenorrhea and
Galactorrhea
62. Inceased protein in HLA and
occurs in RA and SLE
63. 20% of bilirubin is from
64. bilirubin mg/dL to mmol/L
65. protein in HLA- increased in
inflammatory disease
66. urobilin is a color pigment
for…
67. icterus index solution
68. Protein with the most
important function if
maintaining H2O distribution
69. ketogenic, except
70. reacts with histidine
tryptophan and tyrosine
71. not contributing to serum
protein as a result of
pathogenic process
72. specific dye for albumin, used
in immunodiffusion reference
mtd
73. anticonvulsants except
74. 60-70% balances buffer
system
75. categories of steroid
hormones except
76. used to treat acidosis caused
by methanol intoxication
Decreased
Prolactin
B-2 Microglobulin
a. destruction of hemoglobin
b. heme- containing globulins
c. both d. neither
Multiply with 17.1
?
Stool
a.
b.
c.
d.
8.5% normal saline
Sodium citrate
0.01% Potassium dichromate
Alkaline ferrous hydroxide
a. Insulin b.Albumin c. Keratin
b. Transferrin
a.Lysine b.leucine c.aspartate d.acetyl CoA
Phenol method/folin ciocalteau, Kjehldal
method, Folin-Lowry
Increased globulin
Increased serum albumin
Infection
Malignancy
HABA…bromcresyl purple…methyl
orange…bromvcresyl green
Phenobarbital, valproic acid, phenytoin,
carbamazepine,ethosuximide,procainamide,
quinidine, digoxin,
a. Hb/oxyHb 30%
b. Bicarb/carbonic acid
c. Protein buffer
d. phosphate
A. catecholamines
B. Mineralocorticoids
C. Glucocorticoids
D. sex hormones
sodium bicarbonate
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77. best confirmatory test for
drugs
GC/MS
78. best position in venipucture
Doggy style JOOOKE seating upright is the
correct answer
79. analyte that doesn’t need
prior fasting
80. most important factor in
therapeutic drug monitoring
81. anion gap is used in what
disorder
82. treatment for narcolepsy that
increases mental alertness
83. what should RMT do if there’s
excess heparin
84. precipitant of LDL & vLDL for
HDL analysis
85. in glass type collection,
increased heparin causes…
86. coomasie blue G50 is used for
87. slight hemolysis causes
significant changes in…
88. Stress prior to veni, leading to
hyperventilaltion causes…
89. What element is reduced at
cathode in Clarke
Polarography
90. Which does not match
91. Quantification of protein
following electrophoresis
92. Method that has the
secondary monochromator
and the detector at the right
angle
93. Lyophilized reagent to make
standards and controls
94. BUN is synthesized in which
organ
CHOLESTEROL
Timing of specimen collection
Metabolic Acidosis
Amphetamines
Run the sample
Recollect?
Pipette excess heparin
Discard specimen
Dextran sulfate…heparin-magnesium
Increased pHI,increased pCO2
Decreased pH Decreased pCO2
CSF Albumin
a. K+
b. Ca+
c. Na+
Acid-base alteration
a.
b.
c.
d.
e.
f.
g.
h.
i.
Silver
Oxygen
Chloride
Potassium
Spectro-transmittance
Nephelo- scattered light
Aes-emitted
Aas-absorbed
NOTA
Densitometry
Fluorescence
Type II Water
Liver
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95. Oral contraceptives affect
these tests except
96. End product of Caraway is
colored
97. Proficiency testing programs
for (Kind of QC)
98. Cofactor Enzymes except
Pt, APTT, HBA1c, TSH, T3, T4
Blue
Interlab, Intralab
Zinc, maganese, calcium, chloride
Clin Chem October Batch 2016 2nd take
1. Reagents of Liebermann-Burchard
2. End Color of Evelyn-Malloy
3. Most heat stable ALP
4. Most Tissue Specific Enzyme
Sulfuric Acid, Acetic Acid, Acetic Anhydride
Pink, Blue, Yellow, Colorless
Placenta, Bone, Liver, Intestine
Alcohol Dehydrogenase, LFH, ALP, CK (Alcohol
dehydrogenase is LD-6 which can be tissue
specific. If Alcohol Dehydrogenase is not the
answer, considering that LFH is just a typo of
LDH, then, among the three, CK is the most
specific)
5. Hormone used to Monitor fetoplacental unit Estradiol, Estriol, Prolactin, HCG
6. Principle of Polarographic method
The higher the glucose, the more oxygen
depletion
7. Biuret total protein dependent to…?
Peptide Bond
*Protein biuret method measures through?
8. Contribution of NaCl and Bicarb to serum
92%,(based only upon my judgment) 15%,
osmolality
45%,
9. Normally increase in males than in females Estrogen
except:
10. Not increased in non-fasting specimen:
Potassium
11. High Sensitivity, positive in px with
High true positive, Low false neg; High True
Disease
positive, high false negative; False positive, Low
false negative
12.Std:15, Unknown:30, Std Conc:200.
400
Unknown Conc:____
13. Incorrect formula of Abs
A=-log(%T)
(should be 2-(log%T)
14. Flame photometry principle
Emitted Light, Absorbed Light
15. ISE for Potassium
Valinomycin derivative, Crown Ether, Both,
Neither
16. Gluconeogenesis
Glucose from non-carb sources
17. DM type that manifests lter in life due to
Non-insulin dependent
physical inactivity, obesity. Glucosuria
18. Glucose metabolism over 3 month period
HBa1c
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19. CSF Electrophoresis for demyelinating
disorders pattern
20. Glucose lost in glycolysis
21. Can be used for washing lab glasswares
except:
22. Acetyl-CoA is from which 2 compounds
23. NV of Serum TAG
24. CSF Turbidimetric Method uses
25. CSF Pattern in Electrophoresis
26. Increased levels of ALP are normal in:
27. Second largest fraction of anions
28. Deficiency of the enzyme,
Sphingomyelinase
29. Increased in acute Pancreatitis
30. Condition where there is a split in the
band in albumin
31. Normality of a 5 molar sulfuric acid
Solution
32. Characteristic of Multiple Myeloma except:
33. Quantification of TAG is based on:
34. Conjugated Bilirubin
35. Formula of VLDL
36. Cushing’s Syndrome results in
37. Which test uses Benzoic acid for
detoxification function of the Liver
38. Associated with ISE, except:
39. Analyte that has circadian cycle:
40. Analyzes Quality control data
41. What is utilized by spectro to generate a
narrow band pass
42. Lung and Colon Carcinoma
43. Increased Heparin
Monoclonal, Oligoclonal, Polyclonal
2mg/dL, 4mg/dL, 6mg/dL, 10mg/dL
Detergent sol’n, Bichromate, K2Cr2O7 and
conc. H2SO4?, Distilled H20, None?
Glucose and TAG, Creatine and Purine, Glucose
and Protein,(this answer is only based upon
my judgment) TAG and Protein
60-150mg/dL X .0113 = around 0.61.5mmol/L
Sulfosalicylic Acid
Decreased globulin, Increased albumin,
Oligoclonal gamma, monoclonal gamma
Growing Children, Acute Liver disease,
Cancer, Osteogenic Sarcoma(paki confirm?)
Bicarbonate, Chloride, Organic Acid,
Phosphate
Gaucher’s, Fabgs, Niemann Pick, Tay-sach
Amylase, Lipase, Aldolase, ALP
Prealbuminemia, Bisalbuminemia,
Albuminemia
5N, 10N, 8N
Hyperalbuminemia, Hyperproteinemia,
Hyperglobulinemia, monoclonal gammopathy
Chylomicrons, Glycerol, Fatty Acids,
Triglycerides measured directly)
Water-soluble, Directly read in diazotized
sulfanilic acid, Excreted in both urine and stool,
AOTA
Tag/5
Hypernatremia and Hypokalemia
Hippuric Acid synthesis test
Respond to aqueous solution, Responds to one
type of ion, Sensing membrane, all of the above,
NOTA
TSH, Cortisol, Albumin
Westgard, Levey-Jennings, Youden, AOTA
Prism and Diffraction Grating, Filter
CEA, A-2Macroglobulin,
Decreased pH
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Clinical Chemistry October Batch 2nd take
Method of choice in quantitating protein? nephelometry
(densi,fluoro,nephelo,turbid)
In hyperlipidemia 260 --trygly 120- chole ----- High Tryglyceride (<150mg/dL)
interpretation
Cholesterol (<160mg/dL)
Anticoag
that
prevents
glycolysis
(edta, fluoride
fluoride,heparin, double oxalate)
HDL ------TAG- 3% chole- 5%
proteinCrea in m/min UC 120 SC 1.5 total urine 1800 ml
Distance of 2 waves
Wavelength
Turbidity suggest (chole,total protein,albumin, Chylomicrons
chylomicrins)
Treatment for carbon monoxide poisoning (100, 75, 100
50,25% of O2)
Normal of blood sugar
70-110
In serum electro 60% albumin , 5-10% other factors (cirrhosis,
monoclonal
gamma,
if,....30% albumin,4-10%others and gamma 45%
inflammation, CLL)
Protien ref. value
Total protein 6.5-8.3 g/dl if in CSF 1545 mg/L
Quality cntrl of rgt strip
Glucose renal threshold
160-180 mg/dl
PRINCIPLE androgen formed by adrenal cortex and DHEA
the least potent in the androgens
Best for protein determination
nephelometry
Color for jendrasik groff
Diazo reagent (purple azobilirubin)
th
4 number in enzyme classification
1st-category
2nd-subclass
3rdthsubsubclass 4 serial number
Nomal in uncompensated metabolic acidosis
pcO2
Most anodic form
HDL
Glass and needle lancet are disposed where
Puncture proof container
Measure of closeness of repeated analysis
precision
Hydrolase except: ACP, ALt,5N, PCEH,ALT,SCHE, LPS, ALT
AMS
Only hormone secreted by posterior pituitary
Vasopressin and oxytocin
Icterus index solution
0.01% potassium dicromate
Ion gap is used in what disorder
Metabolic acidosis
After electrophoresis in measurig protein, what’s
next?
Protein is measured based on ?
Increase parathyroid, increase calcium
Prostratic Acid Phosphatase
Thymolpthalein monophosphate
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MICROBIOLOGY 2nd Take
Pontiac fever
Legionella (Pontiac had a legion of
demons)
Elek test
C. diptheriae
Enterococcus except
Lactose fermenter
Misidentified as faliciparum
Babesia
Ixodes tick
Babesia
Lactose (+)
Vibrio vulnificus
Inhibitant of P. westermanii
Lungs
Tumbling motility and Gay Bowel
Giardia lamblia
Syndrome
Campylobacter associated
Gastroenteritis
Ingestion of RBC
P. falciparum
No cyst stage
Dientamoeba fragilis
Entameoba gingivalis
Summer diarrhea and Kanagawa
V. parahymoliticus
Niesseria selective medium
Thayer Martin
Clostridium defficle
Exogenous anaeorobes
Medusa head and inverted pine tree
B. anthracis
Zeimanns stippling
P. malariae
;pleomorph
Most common intestinal helminth
Ascaris
Ixodes tick
Babesiosis
Route of infection through cuts/scratches
Erysipelothrix Rhysiopathiae
of skin
Resistant to salt,pickling,smoking
Works involve in handling fish
Non bloody diarrhea
ETEC
nd
2 most common after enterobacteriaciae Pseudomonas
Addition of salt allows the growth of
Selective
specific organism
Tight coil with hookends
Leptospira
Primarily infects large RBCs
P. vivax (primarily interested in
reticulocytes only. Retics are slightly
larger than immature RBCs. Yun lang 
hahaha )
Malaria determination
Through Thick and Thin. (Reference
Bailey and Scott)
MICROBIOLOGY 1st take
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Black centered, green colonies on hektoen Agar
Salmonella (Green salmon colonies
with black toppings  Kain tayo)
Selective medium for Corynebacterium
diphtheriae?
Tinsdale ay selective & diferential;
other mediums are cystine
tellurite agar, leoffler and pai
slant. Pero hindi sure kung ano
yung nasa choices wala kasi nilagay
Stage of Amoeba, non-motile, non-feeding,
infective?
Causative agent of undulant fever?
Cyst
Which of the following is not an arthropod-borne?
Marburg (Sheperd’s hook
morphology)
Seagull appearance, darting motility in hanging
drop?
Campylobactereae (Let’s camp by
the sea)
Which of the following is a transport medium?
Chagoma?
AMIES. Other transport mediums
are JEMBEC, Gono pack, Bio bag,
and transgrow these are used in
Neisseria
Kissing bug (Trypanosoma cruzi)
Entero test, parasite inhabiting in?
Small intestine
Typhoid fever 1st week sample
Blood; 2nd week Stool
Anticoagulant Blood Culture
Sodium Polyanethol Sulfonate
BACTEC First use was for culturing _____
Mycoplasma
Flask shaped meningoencephalitis
Naegleria Fowleri
Green Cellophane Kato Thick Smear
Background an for ova .. :)) hahaha
:P
E-coli strain most common in travelers
ETEC (Traveler’s T. This is also the
form for non-blood dysentery)
Antibiotic no longer usable
Clinical Resistance is antibiotic no
longer usable. Biologic resistance is
the normal features that an
organism can have such as
Brucellosis
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increased rate of excretion of an
antibiotic chuchu.
Ocular; blinding worm
Onchocerca volvulus. (Answer is
not loa loa which also affects the
eyes and is one of the options  )
Nosocomial g(-) oxidase positive rod
Pseudomonas aeroginusa
M. Tuberculosis # of bacilli?
Most sensitive (PCR) 1-10 bacilli;
sputum smear 10,000 bacilli
EHEC asso. With?
Processed meat,
Which schistocyte is found in urine?
S. Haematobium
Ground/ drew itch?
Necator americanus (Hookworm)
Bubonic plague?
Yersinia pestis (Bipolar  Gram
stain: gram-negative rods
exhibiting bipolar staining
Catalase: positive Oxidase: negative
Urease: negative Indole: negative )
Unfertilized egg. Male or female population?
Male
G (-) diplococcus?
Neisseria
Mansonella
Unsheathed, nucleus extend to the
tip
Primary cause of epiglottitis
Haemophilus influenzae
Undergo exflagellation of mosquito host
Microgametocyte? (Basta hindi
yung macro)
Mucolynic soln. For highly viscous respiratory If question just asks for mucolytic,
specimen
go with N-acetyl-L-cystine. If it
mentions decontamination also,
NaOH might be a viable option.
Trypsin is most likely not the
answer.
Ff. True about agar
Primary stain for G/S
Crystal Violet
Antibiotic associated pseudomembranous colitis
Clostriduim difficle
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Non pathogenic mycobacterium
Mycobacterium gordonae
Ingestion of embryonated ova, what infection?
Trichuris trichiura or Cap.
Philipinnensis; pili nalang kayo
kung anong nasa choices
Centrimide agar is selective for
Gram (-) bacterium Pseudomnas
aeroginosa
Bipolar stain w/ methylene blue
Yersinia pestis
Travellers diarrhea
ETEC
Charac. Of strep. D
PYR, bile solbility and 6.5 NaCl (-);
bile esculin +; optochin resistant
Semi quanti. Catalase agent for mycobacterium
Lowenstein jensen
Princ. of catalase
Hydrogen Peroxide production
chuchu
1st step in antimicrobial susceptibility testing
Inoculum preparation
Peanut shape, flattened bipolar drugs
Capillaria philippinensis
Spore forming, anaerobe, indole +, lecithinase -,
lipase -
Clostridium tetanii
Progressing morphology of malarial para.
Gram +, non-motile, catalase -, work involve
handling of fish
Erysipelothrix rhusipathiae
2nd interm. Host of D. latum
Fish
Check of incubator
daily
Dec. Killing of microorganism, EXCEPT:
Long contact,
Primary amoebic meningoencephalitis
Naegleria fowleri
EMB inhibit
G+
1st infection - 1st manifestation
clinical
G+ coc, catalase -, bile solubility (-)Alpha hemolysis
Optochin Resistance first and then
Bile Esculin
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MICROBIOLOGY 1st take
Burned choc.
Proteus
Reproductive of cestode
proglotids
Malarial detection
thick
4 days delayed? Degree?
-20 or -70 pg.15. Kanta nlng dw
kayo.
swarming
proteus
Blood detection, EXCEPT:
Proteus
6 hooklets
Hymenolepsis nana
Larval stage trematode, interm. Host?
Miracidium
Major cause of Diarrheal in human infants and
young animal
Rotavirus (rotavirus makes their
stomachs rotate? Whatever  )
5 days colony?
Fast grower; less than7 days- fast
Can replace India ink
Nigrosin
Crystal violet in cell wall?
Peptidoglycan
Swimming pool conjuctivitis & acute respiratory
dse
Adenovirus; rarely chlamydia
(Aden the swimmer)
Brucella undulant fever? specimen?
Blood (Fever blood)
Choc. Agar identification
Nonselective, enriched and non
differential
Vietnamese farmer
Burkholderia pseudomallei (Buko
farmer)
1st intermediate host of trematode
mollusk
Dysentery in e coli
EIEC (the bloody version since it is
invasive)
Campylobacter vs helicobcter
Urease
Isolation of N. gonorrhaea
Thayer Martin
Perianal swab
Enterobius vermicularis
Virus charac.
Posses DNA or RNA but no both, do
not possess ribossome, not
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susceptible to antibiotic,
intracellular
Primary amoebic meningitidis
Naegleria fowleri
AFB decontamination EXCEPT:
Autopsy
IDA
Hookworm; Ancylostoma
duodenale or necator americanus
Trematode infect. Stage in human?
egg
LOS of H. influenza
Paralizing on the sweeping effect of
Ciliated resp. Epithelium
Food contaminated of cat feces
toxoplasmosis
P-nitroaniline
Cutaneous larva migrans
Ancylostoma duodenale
Gene transfer, “donor cell”
conjunction
Inverted pine tree
anthrasis
Antigenic susceptibility
chlamydia
Favic chandeliers and chlamydospore
Trycophyton schoenleinii
Most sensitive AFS
Rhodamine auramine
Do we accept diapers in Parasitology?
Confirm lang kay Sir to.
MICROBIOLOGY OCTOBER 1ST TAKE
1. Agent of Katayama Fever
2. Reproductive Units of Cestodes
3. Operculated cestode eggs found in
Human feces
4. H. Diminuta life cycle is similar to
5. D. latum is acquired from
6. For taenia solium, man is:
7. Indole (+) extensive swarming
8. Gram (+) cocci, bile solubility sensitive
9. Gram (+) Bacilli, precipitin arch in KL
virulence agar
10. Gram (+) cocci, Catalase (-) PYR (-)
S. Japonicum
Proglottids
D. Latum
Raillietina Garrisoni (Demeaning rails)
Ingestion of fishes
Intermediate and Definitive host
Proteus mirabilis
Next test is 6.5% NaCl?
Corynebacterium Diphtheriae
S. agalactiae
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11. Eosinophilia, Enlarged testicles,
inflamed lymph nodes (eto ata ung from
india)
12. Differentiate E.coli from E. histolytica
(anung meron sa former na
nagdidifferentiate)
13. Stages of Leishmania
14. Borrelia and Babesia ectoparasite
vector
15. With plasmodium, What happens in
definitive host
16. 24 hour urine specimen can be used to
recover
17. Stool sample stained with iodine can be
used to detect trophozoites.
18. Purpose of green cellophane in kato
thick
19. Not acceptable specimen in para lab
20. A collection system that contains
saponin to liberate intracellular organism
21. Due to fragile nature of Neisseria,
transport system includes:
22. Hugh and Leif formulation, open tube
yellow, closed tube green. What type?
23. To improve microbial yield from highly
proteinaceous specimen, digestion and
decontamination is required, Which
specimen requires decontamination only?
24. Selective Media for Gonorrhoeae and
Meningitidis
25. American had vacation. Non-bloody but
watery stool
26. Camp (+) result
27. Gram (+) cocci, Catalase (-), Partial
hemolysis, Bile solubility sensitive
28. Form branched hyphae
29. Gram (+) cocci, Catalase (-), PYR (-)
30. Vibrio Cholera Classic and El Tor
31. Beta-Lysin producer ng CAMP
32. Protozoan that does not contain cyst
Microfilaria
Eccentric karyosome
Amastigote and Promastigote
Ticks
Gametogony (Di ko sure if pwede din
sporogony or if sporogony lang) (basta
definitive ung mosquito)
S. Haematobium eggs
True
As background for ova
Diaper? Confirm with Sir
Isolator-Lysis centrifugation system
(Saponin Isolator)
JEMBEC, Cary Blair, butzler, gaspouch
Oxidizer
Autopsy tissue, Sputum, Lavage, Gastric
washing
Modified Thayer Martin, Choc
ETEC (nag travel kase to)
Increased hemolysis
S. pneumonia (kung staph, strep, entero
choices edi strep)
Katulad rin ba to ng #34? Haha. Ayun ba
ung complete?
Di kumpleto huhu. Pwedeng group D strep
kung hindi Beta hemolytic or S. agalactiae
kung beta hemolytic
O1, O129, O139, O157
S. Aureus
T. Hominis and E. Gingivalis
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33. Gram (+) Cocci, Catalase (-), Bile
Solubility (-), PYR
34. Weakly acid fast transmitted through
inhalation (soil and dust) with branched
hyphae
35. Swarming, Anaerobic
36. Inhibit saprophobic fungi and bacteria,
respectively
37. Urea agar, pink
38. Virus general characteristics
39. Cold Agglutinins/ Anti-I
40. Fungal storage temp
41. Tinsdale agar
42. Purplish in Mcconkey. G(-). Oxidase (-).
Motility (-)
43. Does not grow in Mcconkey
44. Methylene blue in EMB inhibits
45. Choc is what
46. The following specimen processing is
correct except
47. Yellow colonies for lactose
fermentation
48. Reagent for Nitrate Reduction test
49. Vietnamese farmer
50. Agar for the V factor in Haemophilus
51. PCR test not used for amplification
52. Most common way of sterilizing in
small hospitals
53. Sterilization using autoclave except:
54. Reye’s is associated with:
55. Most commonly used and most costeffective cell culture
56. What are the CPEs
Anu to behh huhu. Kung (+) sa PYR,
Eneterococcus kung (-) Group D strep.
Ganern na lang.
Nocardia
Cyclohexamide and Chloramphenicol
T. Mentagrophytes and Crypococcus
Intracelllular, Do not produce
ribosomes, Not susceptible to
antibiotics, usually stable at ph 5-9,
possess DNA or RNA basta its not
generally unstable after recombination
Mycoplasma Pneumoniae
25-30C
C. Diphtheriae
Acinetobacter
Aeromonas, Plesiomonas, Listeria, E.coli
Gram (+)
Nonselective, Enriched, Nondifferential
Compare to Mcfarland, 15 minutes, use
same isolate, Direct is not appropriate
for fastidious
XLD
Sulfanilic acid
Burkholderia pseudomallei
Choc agar
DNA Probe, RNA Primers, Taq, Pfu
Steam under pressure
BA, EMB, XLD, Choc
Orthomyxoviridae, Paramyxoviridae,
Picornaviridae, Coronaviridae
Tissue/Cell culture
Necrosis, syncytial formation, cytoplasmic
vacuolation basta ALL OF THIS
M. gordonae
57. Mycobacterium nonpathogenic sa tap
water
58. Multiple tube fermentation test positive Gas production
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59. Lab results ng M. tuberculosis
60. Anaerobic indicator turning to pink
61. For anaerobic specimen, which is
allowed
62. What is not an appropriate collection
method
63. Anticoagulant for viral culture
64. 4C preserve except:
65. Color ng G(+) bacteria when crystal
violet, iodine, and acetone-alcohol is added
66. Fastidious bacteria
67. Low viscosity, Low Yield, High Volume
68. Which of the ff is not true about disk
diffusion
69. Lancefield grouping specificity
70. Automated in susceptibility
71. Variables in MIC
72. Who decides on formulation of
Antibiotics in testing
+ Niacin, +Nitrate reduction +Catalase na
heat labile (hindi heat stable)
Resazurin, Methylene Blue
Venous blood, catheterized urine, sputum
Removing dentures, use calcium chloride
for tissue, unsterile but washed stool,
fresh would using sterile swab, NOTA (not
sure of the answer)
Heparin
Bacterial blood specimen, Viral blood
specimen, catheter, urine
Purple
Enriched
Centrifuged, Single drop, Layered
The great the area of inhibition, the more
susceptible; Too thick MH may cause false
resistance, Light inoculum may cause false
susceptibility, The narrower, the lower
amount of antibiotic must be used
C Carbohydrate
Vitek, Alamar, Epsilomer, Spiral Gradient
Incubation time, Inoculum size, growth of
bacteria, AOTA
Medtech, Infection department, Pharmacy,
AOTA
73. Mycobacteria grows in just 5 days
74. Sensitivity of Sputum in mycobacteria
75. Amount of Stools in Cholera
76. Katothick. Brown bipolar plugs
77. Heart-lung migration of roundworms
Rapid grower
10,000 bacilli
10-30, 5-10
Trichuris trichiura
Ascaris-Trichuris, Trichuris-hookworm,
Ascaris-Hookworm, Strongy-Trichuris
78. Lateral prominent spine
S. mansoni
79. S. japonica loses its tail in circulation
Schistosomule
80. Filamentous. UTI because of IUD
Actinomyces
81. Develops red color for nitrate reduction Positive
test?
82. Differentiates helicobacter and
Ung may Urease
campylobacter
83. Latex agglutination of S. aureus due to
Clumping factor (coagulase) and Protein A
84. Patient has chagoma, what is the
Kissing bug, Sandfly, Tsetse, Ixodes
vector?
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85. Which helminth eggs will not float in
zinc sulfate flotation method?
86. Sensitive test to quantify plasmodium
87. Which of the ff organisms does not
match
88. Blood can be helpful in the ff except
89. Can replace india ink
90. Autoclave of soiled materials
91. Agent that is most resistant to heat,
chemical and radiation
92. Characteristics of Nematodes except:
93. Soil-transmitted helminthes except:
94. Help each other to protect
95. Indole (+) lecithinase (-) Lipase (-)
96. TCBS, Will color yellow (lactose
fermenting)
97. Deerfly tick
98.(--++) Motility (+) wound H2s (-)
99. Semicritical materials, why sterilize
100. Decarboxylation results in
101. Major indicator for fecal
contamination
102. M. perstans
103. First intermediate host of trematodes
Unfertilized ascaris
Thick blood smear, Thin blood smear,
either, neither
O. volvulus-skin snips, Echinococcus-stool
Babesias, filariasis, Cysticercosis,
Trypanosomiasis
Nigrosin, Giemsa,
30 minutes, 15 minutes
Spores
Incomplete digestive tract
Capillaria philippinensis
Biofilm
Clostridium tetani
V. Vulnificus
F. Tularensis
Enterobacter Cloacae, Klebsiella
prenumoniae
Mucous membranes
Amines, Tryptophan, CO2
E. coli
Unsheathed, nuclei extend to tip
Mollusk
MICROBIOLOGY 2ND TAKE OCTOBER
1. Antibiotic-associated
pseudomembranous colitis
2. Canned good bacteria
3. Can be seen with Brightfield Microscopy
4. P-dimethylaminobenzaldehyde is for
detecting
5. Ringworm of the Scalp
6. Horteaea Werneckii; dark patches on
palms and soles
7. Late Lactose Fermenter. Red pigment;
Prodigiosin
Clostridium Difficile
Clostridium Botulinum
Leptospira, Borrelia, Treponema, NOTA
Urease, M2S, Indole, Oxidase
Tinea Pedis, Tinea Capitis, Tinea Cruris,
Tinea Manuum
White Piedra, Tinea versicolor, Tinea
Nigra, Black piedra
Citrobacter freundii, Enterobacter cloacae,
Pseudomonas aeruginosa, Serratia
marsescens
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8. Yellow (+) indicator of carbohydrate
metabolism
9. Greenish discoloration of surrounding
colonies
10. Erythema chronicum migrans is
associated with
11. Bile Esculin is for:
12. Falling leaf motility
13. Visceral Larva Migrans
14. Most common anaerobic bacteria
15. Fried-egg appearance
16. Agent for infectious mononucleosis and
asso. With Burkitt’s lymphoma
17. Egg-based agar for Mycobacterium
except:
MSA, EMB, MAC, HE
Alpha hemolytic, Beta-hemolytic
Erysipelothrix rhusiopathiae, strep
pyogenes, ricketsiia ricketssiae, Borrelia
burgdorferi
Haemophilus, Brucella, Enterococcus,
Staphylococcus
Giardia Lamblia
Leishmania, Toxocara canis
Bacteroides fragilis, Clostridium tetani,
Clostridium botulinum
Mycoplasma, ricketssia
EBV, Cytomegalovirus
Lowenstein Jensen, Middlebrook,
Petragnani, ATS(American Thoracic
Society)
18. Rocky Mountain Spotted Fever
Rickettsia rickettsii, Orientia
tsutsugamushi, R. prowazekii, R. typhi
19. Differential medium for non-fermenting ONPG, Choc, EMB, OF
gram (-) which do not acidify TSI
20. Causes of mumps/inflammation of the Picorna, Rota, Paramyxo, Orthomyxo
parotid glands
21. Gram(+) cocci, catalase (+), novobiocin Staph saprophyticus, Staph epidermidis,
resistant, common among sexually active
Group C strep, Enterococcus faecalis
females
22. Isolator-Lysis Centrifugation System is Intracellular organisms, L-forms,
used to detect:
23. What specimen is used in Brucella?
Blood, Stool, Urine, Sputum
24. Bubonic plague
Yersinia pestis
25. Soft Chancre. Gram(-) Coccobacilli with Neisseria gonorrhoae, Haemophilus
tangled chains
ducreyi, Treponema Pallidum
26. Culture media for Antimicrobial
Mueller-Hinton Agar
susceptibility
27. Pink growth in Urea Agar
T. mentagrophytes and Cryptococcus
28. Parasite for Iron deficiency Anemia
Hookworm, D. latum
29. 6 hooklets egg
Trichuris, Saginata?, Ascaris
30. Decolorizer omitted, Streptococcus,
Purple, Purple; Red, Red; Colorless, Red;
Neisseria will stain?
Colorless, Colorless
31. Differentiate Staph and Strep
Catalase
32. Bacterial blood culture anticoagulant
SPS, Heparin, EDTA
33. D. Latum
Infected Raw Fish
34. Barber’s pole appearance na parasite
Parastrongylus cantonensis
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35. Cuticle expansion of E. vermicularis
36. Non-hemolytic, catalase (+), coagulase
(-), gram (+)
37. Not acceptable for anaerobic culture
38. Microorganism that requires increased
CO2
39. Autoclave
40. Decreased number of microbial killing
EXCEPT
Cephalic alae
Saprophyticus/Epidermidis
Catheterized urine
Capnophilic
121C at 15minutes
Longer contact time (this will increase
kasi)
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URINALYSIS 2ND TAKE JANUARY
Gastric produced by
gastrin
DI is caused by decreased
ADH
Not found in UA lab
Waterbath
Viscosity of synovial fluid is due to
Hyaluronic acid
Sugar of semen
Fructose
Most component of urine
Urea nitrogen and creatinine
Can interfere ascorbic acid
BBLNG
Pathologic crystal
Principle of SG rgt strip
Measure of pKa change of certain pre
treated polyelectrolytes in relation to ionic
conc. Of urine color of rgt (blue-green)
Presence of RBC but not seen
Hemoglobinuria
microscopically
Cast are formed in
DCT
Maldigestion and malabsorption of
d-xylose
steatorrhea
HCG detected after conception
6-8 days
HCG undetected after delivery
2 weeks or 14 days
Cause cloudiness immediately upon
WBC / lipids?
voiding
Cause turbidity
Milky fluid
Prostate gland
Positive red spot sa ovary ; infection into
FRIEDMANNS TEST
vein of marginal ear
Transudates characteristics
Phenylketonuria increased in
Phenylalanine hydroxylase
Wethstone acidic crystal
Uric acid
Fat, except:
sulfoacetic acid
Ethelene glycol
CAOX
Bilirubin interference
Porphyria cutanea tarda
Port wine
Steatorrhea droplets
>60droplets/hpf
PSP measures what ??
Tubular secretion and renal blood flow
Heat and acetic acid
Protein
Main matrix of cast
Tamm hors fall
Decreased alpha glucosidase
Can pass thru glomerulus to become
RBC
filtrate
Copper sulfate reduction test
Glucose
Not normal seen but when seen is
RTE cell
significant
w/o diffraction halo
Interference contrast
Coomasie brilliant blue G250
Urine protein and CSF
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Used to detect sputum and saliva
Purple rx
Medtech second reading no casts
For prostatic infection
Thorny apples; urea splitting bacteria
;alkaline
Clinical significance of testing serum HCG
in male
Dust cell/carbonleyden
Leukocyte esterase
Increased Ph
3 glass
Ammonium biurate
Testicular tumor
Albumin not meant to be filtered through
glomerulus…
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UA
Cloudiness of urine
p.23
Transudates, EXCEPT:
malignancy
Principle of rgt strip
Color change
Normal pH of random urine
4.5-8.0
Red cell cast? must contain free red blood cell?
T or F
Urinary sediments treated?
Acetic acid, NSS, K, iodine
Acid phosphate
prostate
Ascorbic acid interference
Scrappig in diaper
cammidge
Ammonia odor in urine
Test under hood
Hormone for reabsorption of h2o
Aldo?
Tube 2 CSF
microbiology
Alkaptonuria
alkaptones
Incorrect pairing
Ketone-dm?
black
melanuria
Male back pain
Acute glumerolo.
Cryptococcus neoformans
India ink
Decrease sg
Diabetes insipidus
Type of microscope object will appear bright w/o
diffractions HALO
Interference contrast
High in urine not in body fluids
Urea, crea, salt
Lung kidney
Good pasture, wegeners
Nitrate to nitrite
For blood culture
Bacterial culture
Midstream catch
Formulating creatinine clearance
C= UV / P x 1.73/ A U- URINE CREAININE mg/ dL
C= CLEARANCE ML/ MIN
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V= VOLUME OF URINE
Characteristics of ideal preservative
Bactericidal
Inhibits urease
Preserved formed elements in the sediments
Dont interfere w/ chemical tests
Speckled
Nonpathologic
Hyaline casts
Heat & acetic acid
Test for albumin (protein)
Cryptococcus neoformans
India ink
UA
What to do with a specimen pH at 9.0
recollect
Rare mousy odor, presence of?
phenylketonuria
Procedure for collecting ascites?
P.125
Vacuolated macrophage engulfed a neutrophil?
reiter
Screening test for asymptomatic bacteriuria?
Leukocyte?
Creatinine clearance; urine creatinine = 150 mg/
dL ; plasma creatinine = 128.50 mmol (di ako sure
sa unit), volume 24 hr
1.2L
Non-pathogenic crystal, alkaline urine, presence of Amorphous urate
ammonia, caused by urea-splitting bacteria
5 small RBC square, specimen count, multiplied by? 1M if 2 wbc large sq = 100,000
(yung 10,000, 100,000 ganern)
2 WBC (sperm)
100 000
Ammonium biurate,
Spherical, thorny apples, prismotic
specules
Confirmatory for bilirubin?
ictotest
Wrickled blood result as positive sa dipstick?
Hematuria?
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Contamination of amniotic fluid with fetal or
maternal blood effects
Increase in bilirubin?
Traumatic tap features. Differentiate hemolysis in
traumatic tap from non-traumatic
Read table p.87
S.G. urine strip principle
Alkaline medium
Pernicious Anemia Gastric Testing Chemical
Stimulant
Histamine phosphate test
(What is the chemical used)
L/S ratio true or false (When does formation start)
Indicates fetal lung maturity (L= 35th)
(S= 26th)
83. B
Malabsorption malformation stearrtorhea What
test do we use. Apt test / Lactose intolerance test
(fecal test- van de kamer confirmatory
test)
Apt test- diff. Fetal and maternal blood
Diabetis Insipidus
Defect in insulin, vasopressin, ADH
Blood reagent strip true or false
Causes of speckling on blood reagent strip,
Effects of methemoglobin on reagent strip
Spinal deformity adult sight of collection
General substance not allowed in
glomerular filtrate
Protein not allowed.
Meningitis classification
Viral, bacterial?
Glomerular dse indicator
Red cell
ANCA. Vascular.
Orthostatic proteinuria
1st (-) 2nd (+)
Cant reabsorb water
Wegener’s
Ascending LH
Normal odor of urine
faintly aromatic
Brown amiotic fluid
Severe hemolysis (blood)
Unit of creatine clearance
ml/ min
Urine formation
Crea, urea nitrogen
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Fetal maturity indicator
Alpha fetoprotein
Why 1st voided urine best?
Concentrated specimen
2500ml / 24hrs?
polyuria
stain not true?
Microalbuminuria ration in what reference?
SG, uric acid...
Presence of Hgb; no rbc
hemoglobenuria
Turbid urine EXCEPT:
350 mm3 rbc, 250 mm3 wbc, fat,
ammonium phosphate
leucine
struvite
Ammonium magnesium phosphate
Total vol. Of CSF in adult
90-150ml (10-60 neonates)
Characteristics of urine preservatives
Bactericidal, inhibits urease, preserve
formed elements, dont interfere w/ chem
test
Major nitrogen in urine
Urea-creatine
Inc. In urine that is not in other body fluids
urea
Dysmorphic RBC
Glomerular bleeding
Sperm cnt in thoma pipette?dilution?
100,100 (2 large sq)
1. Gland responsible for releasing ADH
Pituitary gland
2. Order of Renal Blood Flow
Renal Artery. Afferent, Peritubular, Vasa
Recta, Efferent, Renal Vein
3. Included in the Glomerular Filtration
except:
RBC
4. First indicator of renal dysfunction
Glomerular chuchu
5. Which is not affected by unpreserved
urine
Oxidation of Urobilin, transparency,
6. Effect of Angiotensin II, except:
Control of aldosterone which is responsible
for water reabsorption
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7. Determination is specimen is actual urine Urea Nitrogen and Creatinine
8. Rough Determinant of degree of
hydration
Color
9. D. Insipidus
Decreased SG
10. Color of urine upon ingestion of
Pyridium
Amber
11. SG using urinometer at 20o C with 2
mg/dl protein and 3 mg/dl glucose
1.012
12. Major advantage of refractometer to
urinometer
Use of small amount of specimen
13. Causes of acidic urine
Choices:
dehydration, diarrhea, protein, pulmonary
emphysema, vegetarian
Dehydration, protein, pulmonary
emphysema, fever (basta 1,2,3,4)
14. Principle of Clinitest
Copper Reduction
15. Urine pH of 9.0
Recollect
16. (+) clinitest (-) glucose oxidase
Non-glucose reducing sugar present
(galactose, fructose, pentose, lactose)
17. Most indicative of renal disease
Protein
18. Effect on pH if protein pad run over
false lowering of the pH reading
19. Butanol: Top-Red, Bottom-Colorless
Chloroform: Top-Colorless, Bottom-Red
Urobilinogen
20. Hoesch reagent
Ehrlich dissolved in 6 M HCl
21. Nitrate reduction
Choices: g(+), g(-), yeast, AOTA
g (-)
22. UTI—Increased WBC with ?
Choices:
a) (+) LE
b) Increased SG
c) (+) Protein
d) Presence of bacteria
(+) Leukocyte Esterase
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23. Nitrites screening utilities, except:
Choices:
a) screening for urine culture sp
b) monitoring pc at risk for UTI
c) evaluating antibiotic therapy
d) evaluating fasting sp
Evaluating Fasting Specimen
24. Not routinely read at 60 sec
Choices: pH, protein, bilirubin, nitrites
Bilirubin
25. Stain that best enhance the nuclear
detail
Choices:
a) Hansel stain
b) Eosin
c) Toluidine
d) Fat Red
0.5% Toluidine Blue
26. Accumulation of ketone lead to except:
Choices:
a) dehydration
b) alkaloids
c) diabetic coma
d) electrolyte imbalance
Alkaloids
27. Presence of mucus thread in male is
clinically significant. T/F
False diba?
28. Severe liver disease, rarely seen, seen as Tyrosine
clumps or rosette colorless to yellow
Choices:
a) tyrosine
b) leucine
c) bilirubin
d) ampicillin
29. Vital aid in identification of crystals
pH
30. Inumerable RBCs, what to do to enhance Acetic Acid
WBC
31. Not pathogenic
Choices:
a) clue cells
b) bubble cells
c) glitter cells
d) yeast
Glitter Cells
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32. Differentiate Oval Fat Bodies from
Bubble Cell
Choices:
a) OFB: RTE with lipid contents
b) OFB: RTE with non lipid filled vacuoles
Oval Fat Bodies are renal tubular cell
containing lipids
33. Not true about casts
Choices:
a) shape determined by origin
b) high pH
c) found at edge of cover slip
d) immunologic property
High PH
34. Found in prolonged urinary stasis
Choices:
a) epithelial cell cast
b) fatty cast
c) fine granular
d) coarse granular
Fine Granular
35. Not seen in urine after strenuous
exercise
Choices:
a) RBC cast
b) WBC cast
c) hyaline cast
d) granular cast
WBC Cast
36. Cast seen in normal urine
Hyaline
37. Reported semi-quantitatively
Choices:
a) RBC
b) WBC
c) casts
d) crystals
Crystals
38. Appearance of leucine
radial striations & concentric circle
&Grapefruit & Crushed Diamonds
39. Increased HCG with no doubling time
Ectopic Pregnancy
40. Female rat urine is used in these
bioassays except:
Choices:
a) Friedmann’s test
b) Frank-Berman test
c) Kelso test
Friedmann’s test (virgin female rabbit)
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d)
Kupperman test
41. Urea-splitting bacteria UTI is
accompanied by which crystals
Struvite
42. Clinical symptom of renal lithiasis
Pain at the back
43. Percentage of Calcium Phosphate in
renal stones
75%
44. Cause of false (+) fecal occult blood
Sardines, iron, vit C
45. After 1st trimester, major contributor of Fetal urine
amniotic volume
46. Cause of polyhdramnios
Neural tube disorders
47. Cannot be used as fetal lung maturity
indicator
L:S Ratio <2
48. Regulates light intensity in microscope
Rheostat
49. Automation of Urine Pregnancy
VEDALAB Easy Reader
50. Definition of Renal threshold
Amount that the body cannot take na so it
goes out (haha sorry guys iparaphrase ko
na lang) (may spills over daw)
51. Contain black granules; anthracosis
Carbon-Laden (Dust Cells)
52. 50 mg/dl ____; 600 mg/dl _____ to define
urine
creatinine, urea nitrogen
53. Nephrotic syndrome
Choices:
transudate or exudate
Transudate
54. Preservative when used in large
amounts precipitates crystals
Boric acid
55. Which does not immobilize sperm cells? Chloroform
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56. Must be avoided when collecting
Synovial fluid:
Choices:
a) collecting using syringe flushed with
heparin
b) fluid EDTA for cell ct
c) use of powdered anticoagulant
d) collecting less than 10 ml in nonanticoag
Use of powdered anticoagulant
57. Best way to collect sp if px is an adult
with severe spinal deformity
Cisternal puncture
58. Type C fire class
Electrical equipment
59. Biological wastes should be put in
appropriate container with biohazard
symbol except:
Choices:
a) urine
b) sputum
c) stool
d) blood
Urine?
60. Monocyte with engulfed neutrophils
Reiter cell
61. Sputum collection
First morning
62. Maximum amount of amniotic fluid
collected
30 ml
63. Scraping stool from diapers
Cammidge
64. Which does not correlate
nitrate=purple (It should be pink)
65. Osmolarity is better than SG
True
66. SG of Glomerular filtrate
1.010
67. Disinfectant of blood contamination
Sodium hypochlorite
68. Employee spilled acid on arm
Wash with running water for 15 mins
69. Organ affected in Zollinger Ellison
Pancreas
70. Bronchial asthma—All are significant,
except:
Pneumoliths
71. Glucose Renal Threshold
160 to 180 mg/dl
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72. Synovial fluid determination that
differentiate inflammatory and septic
arthritis?
Lactate
73. Cholesterol crystals with normal TAG
Pseudochylous
74. Increased of free fatty acids but normal
neutral fats
Malabsorption
75. Responsible for foul odor of stool,
except:
(skatole, indole, butyric acid)
Urobilin
76. Traumatic tap
Uneven, clear supernatant, clot formation
77. Meningitis with pellicle formation
Tubercular
78. Storage of CSF for cell count
Refrigerator
79. Normal volume of CSF in neonates
10-60 ml
80. Minimum acceptable urine volume in
the lab
10 ml
81. Semen automation—velocity and
trajectory
CASA
82. Best type microscope for hyaline cast
Phase contrast
83. Ascorbic acid causes false (-) in all,
except:
Ketones
84. Reagent of choice for CSF Turbidimetric Trichloroacetic acid
Method
85. Normal Sperm count
20-160 million
86. Parameters measured in semenalysis,
except:
Glucose concentration
87. Alkaline sperm which help neutralize
Bulbourethral gland
the acidity from the prostate secretions and
vaginal acidity come from where
88. Method of urine collection for bacterial
culture, except:
First morning
89. Tubular necrosis odor
Lack of odor
90. pH where urine of px with UTI should
be maintained at
Acidic
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91. Limulus Lysate Test; horseshoe crab;
what is responsible for the blue color
Choices:
a) calcium
b) magnesium
c) copper
d) iron
Copper
92. Semen—color of live cells that do not
take up the stain
Choices:
a) faint red
b) purple
c) blue
d) white
(bluish white sa module) Choose one Blue
or White
93. Which is NOT true in semenalysis
Choices:
a) abstinence 2-3 days not longer than 5
days
b) transport at RT within 1 hour
c) analysis before liquefaction
d) empty bladder before ejaculation
Transport at RT within one hour
94. Broad cast
Grave prognosis
95. Impermeable to water
Ascending Loop of Henle
96. Increased number of squamous
epithelial cells
Improper collection
97. Pernicious Anemia
Histamine Phosphate test
98. Intravascular Hemolysis
Hemoglobinuria
99. (+) clinitest, (-) Reagent strip
Galactose is present
100. Secretion of ADH is dependent on
Level of blood pressure or hydration ata?
101. Avoided when collecting CSF specimen May tanong ba na ganto? :/
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1. Increased in intake of Bananas,
Avocados, Walnuts
2. Urine sediment +_____ = stain pus
3. Leukocyte Esterase
4. Major Nitrogen Containing Compounds
in Urine
5. CaOx
6. Normal Blood Glucose + Glycosuria
7. Effusions;noninflammatory ; No
coagulation on standing
8. Guide in gestational assessment of
gestational age in Amniotic fluid
9. Pregnant women with fresh urine for
Benedict’s test. Formation of deep yellow
color is caused by the ff. except:
10. Turbid Thoracentesis
11. Spermatozoa matures where?
12. Measured by SSA
13. Semen normally liquefies after how
many minutes?
14. Male- back pain. Only characteristic
finding in urine sediment: 10-20 rbc per
hpf and RBC cast. Reason?
15. Multiplying factor for sperm count if
using 2 WBC squares
16. Calcium Carbonate Shape
17. 1.040-1.050 SG means?
18. CA 125 (+) CEA(-) , peritoneal means?
19. Intake of caffeine, alcohol and diuretics
will lead to
20. Ascitic Fluid origin
21. Black stool
22. CSF Pellicle. How many hours at ref
temp.
23. Highly diagnostic of peritoneal with
Intestinal perforation
24. Neural tube disorder has increased:
25. Calcium Carbonate pH
5-HIAA, Aldosterone, 7-Ketoacids, LD
Acetic Acid, Iodine, Prussian Blue,
Purple
Urea, Creatinine, Uric Acid, Glucose
Octahedral Square?
Renal Tubular Damage, D.I, D.M
Transudate, Exudate, Either, Neither
Creatinine, Bilirubin, Uric Acid, CHON
Lactose, Ascorbic Acid, Amino Acid,
Glucose (In clinitest for glucose, Ascorbic
acid will give a False (+) that’s why it is not
the answer.
Nephrotic, Congestive Heart Failure,
Bacterial Infection, Hypoalbuminemia
Epididymis, Seminal Vesicles, Prostate,
Testis
Urobilinogen, Bilirubin, Ketones,
CHON(Protein)
15, 30, 45, 60
Nephrotic syndrome, Congestive heart
failure, Renal lithiasis(renal calculi)
100,000, 1million, 2 million
Dumbbell, Thorny Apple, Coffin Lid
Radiographic Contrast dye (you should
dilute?), Highly alkaline urine
CA 125 is for ovarian origin. CEA is for
gastrointestinal origin
Polyuria, Anuria, Oliguria
Peritoneal fluid
Iron Therapy, Upper gastrointestinal
infection, Bile Obstruction, Barium Sulfate
6-12 hours, 12-24 hours(UA pg. 87), 1-5
hours, 5-10 hours
Increased cholesterol, Increased ALP,
Decreased Glucose, Decreased creatinine
Acetylcholinesterase activity
Ph 8
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26. Phenylalanine to Tyrosine prevents:
27. Exudate Effusions
28. Normal WBC CSF Count
29. Increased Hydrostatic pressure?
30. Lactate >25mg/dL, lympho and mono
in CSF
31. How many days of output of stool for
fat analysis
32. CSF Glutamine (high) Indicates
33. Ketone in urine not detected
34. False positive occult blood:
35. Predominant WBCs in CSF (for
neonates)
36. Where does ACP and proteolytic
enzymes originate?
37. 2nd tube for Microbiology
38. Diluent of WBC count in CSF
39. Struvite
HEMATOLOGY
Phenylketonuria
Pneumonia, Hepatic Cirrhosis, CHF,
Nephrotic
0-5wbc/uL, 0-10, 0-20, 0-30
Exudate, Transudate
Tubercular Meningitis
1, 2, 3, 4
Reye’s Syndrome, Guillain-Barre
syndrome
Diacetic, Acetone, B-Hydroxybutyric
Banana, Prunes, Peanuts
Monocytes
Prostate, Epididymis, Seminal Vesicles
Chem, Microbiology, cell count
0.9% NaCl, Pilot’s Solution, 1% Potassium
Dichromate, 3% Acetic Acid
Magnesium Ammonium Phosphate
HEMA
Spherocyte indices
Mcv= 77-87; MCHC increased
WBC callibration
11
Poikilo/aniso
Shape/size
HIV def cell
t-lymph cytotoxic
Deoxyuridine suppression test
Megloblastic
Slowest to migrate
HbA2
1 megakaryocytes
1000-4000 plts
Alpha thalassemia incompatible with life
Bartsfetalis
philadelphia
CGL/CML
Std. ng prick
3mm
Defective centrifuge not affected
MCH
Gamma chain
Chromosome 11
Replace glutamic acid in Sickle cell
Valine
Cobalamin transport
Cobalamin II
Decreased fibrinogen
Thrombosis
BM aspiration in adult
Iliac
Cytogenetic spn
BM cell
Macroovalocyte is seen except
Hookworm
Function of condenser
Directs the beam of the light in
Lab test presence of sickle cells
Electrophoresis
Malignant cell in CLL
B lympho
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Prolong PT and APTT
Factor 2 activator
Red color ascorbate cyanide
Secondary of hemostasis
Prolonged PT
Does not respond to ristocetin
Factor 7 and 9
5,8, protein C
Presence of G6PD
Coagulation
1,2,5,7,10
Bernard Soulier
HEMA
Immature granulocyte found in PB but small
percentage
Function of condenser
Directs the beam of the light into he
spn.
Splenomegaly, increased or decreased platelets?
Decrease
RBC w/ iron granules in Prussian blue?
Siderocyte
WHO categorize acute as?
>20 bone marrow
PT is used for?hndi factors*
Oral anticoag theraphy
Platelet storage pool defect, alpha granules lacking
Gray syndrome
Shift to the left
Increased O2, decreased 2,3 DPG,
deceased body temp, decreased CO2,
increased pH
Factor IX deficiency is also known as?
Hemophilia B
Fibrinolysis activators
Fibrinogen, PAI-t, tpA, alpha-2
antiplasmin and plasminogen
Monocyte goes towards the tissue. Process Called Diapedesis
what?
ESR factors
Erythrocyte, plasma composition,
mechanical errors
Adams ts 13 associated with?
TTP
Neutrophil to tissue process called what?
Diapedesis
Est. Plt. ct. Magnification used?
1000x
Philadelphia asso with?
CGL
Principal protein of RBC?
Sa peripheral spectrin
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Principal protein of RBC?
Sa integral band 3
3.36 x 10^12 (answer na ata to)
SOLVING TO!!
MM asso with?
Sakit sya sa Plasma cell
F XIII does what? (function)
Stabilizing factor
Platelet clumps Increase or decrease in counts
Decrease
Hemolytic Anemia morphology
Normo/normo
Electrical Impendance Principle
Base on changes in electrical resistant
produce by cell. Voltage pulse is
proportional to the size of the cell.
Pulses is proportional sa numbers of
cells
Most Verstatile Stem Cell
Pluripontent, multipotent etc
CAIHA
Anti body (I)
Hemostatic pathway
Normal Adult Hemoglobin
Pag female 12-16g/dl male 13.5-17.5
g/dl
Factor IX
Hemophilia B
Alkali Denaturation Test
Fetal Hgb
GIIb/IIIa
aggregation
sideroblast
Micro/Hypo or N/N
Methyl blue and..
Eosin
PPP is more hemostasis
Extramedullary hemostasis
Liver, spleen at lympnodes guys .kung
except ang tanong eh di alam niu na
..hahaha !!!
Fibrin monomer polyzmerize to form
Next step po ay yung ASSEMBLY OF
POLYMERS
Asso. w/ vwf
Plt function
Dec. LAP score?
CGL
BT is for
Plt function
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Determination of cell complexity
Side scatter
Flip-flop
PF3 or anti-heparin
Indicator of megaloblastic
Hypersegmented neutrophil
Formula for wbc
#of wbc x df / area correlation factor x #
of square
Dec. Fibrinolytic system
Hodgkins dse
Reed Sternberg cells
Aticoag. For plt cnt
3.2% sodium buffered citrate=most
recommed
VCS
coulter
AMLLS # FAB
8 CML=6 ALL=3
Maximum tourniquet cause hemoconcentration.
1-2 mins
Plt aggregate & neutro, correction done?
Charac. Of retcis
Larger than mature erythrocyte
megaloblastic
Hypersegmented neutro
Forward scatter x, side y saan makikita ang
lymphocyte
Upper right
HEMA
Hbs trait from Hbs dse
HbA
Methd use when you move the blood film in wbc
cnt
longitudinal
Anticog in vit, K
Warfarin
Instrument coulter calculate
It calculates Hct, RDW,MCHC,MCH guys
ang naalala ko dun na lumabas ay yung
HCT
Shift to the right
Decresed affinity O2, increased temp,
increased 2,3 DPG, inceased CO2
decreased pH
Spcmn for plt count
WB
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Alder rielly anomaly
Cytoplasmic abnormalities
Wafarin overdose
Vit. k
1st hemostatic response
vasoconstriction
Normal life span of plts
10 dys
Asso w/ erythroleukemia
Ringed sideroblast
Inc. In hgb A2
Heterozygous beta thalassemia
Source of error most coagulation procedure
AOTA
Heparin can be used EXCEPT:
Rees and ecker
Physical exam of primary hemostatic pathway
Petechia or ecchymoses
Normal hgb
deoxyhgb
WBC uses scatttered light
Abbott cell dyn
1. Anticoagulant responsible for platelet
satellitism
2. Heparin can be used in the following,
except?
EDTA
3. Sites to avoid in patients with DM if hand
is not available
4. Preferred site of collection for patients
<1 year old
5. Drabken’s reagent constituents
Ankle or foot
6. Measurement of Hematocrit that uses
pulse height generated by the RBC
7. What instrument measures WBC count
and differential by scatter light
8. Corrective action of schistocytosis when
suspected from RBC histogram
9. Which methodology uses P. nitroaniline
bound to synthetic oligopeptides
10. Anticoagulant to blood ratio
11. These are the hydraulic components of
automated analysers except?
a. Osmotic fragility
b. Rees and Ecker
c. Eosinophil Absolute Count
d. Acid-base balance
Lateral part of the plantar surface of the
foot, Heel, Earlobe
Potassium ferricyanide + Sodium
bicarbonate
Sysmex
Abbott Cell Dyn
Smear review
Chromogenic
1:9
Aspirating unit, Dispensers, Mixing
chambers, Aperture baths, valve
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12. What is not an advantage of
automation?
13. Iron in an adult is most commonly
found in?
14. In Kleihauer Betke test, the dark cells
indicate:
15. Cells seen in Wright stain except:
16. In granulocytic maturation, secondary
granules are found in?
17. Red brown blood with Heinz bodies
18. Irregularly spaced spicules found in
haemolytic anemia and metastatic liver?
19. Stress induces:
20. In ALL, the presence of large,
homogenous blasts, indicate?
21. Stain for basophil
22. Flared skirt lymphocyte
23. Thrombopoetin is produced in?
24. Platelet stage that has dense granules
formation
25. Most potent agonist
26. Content of alpha granules specific to
platelets?
27. Most sensitive for ESR?
28. Which is not correct with PT?
All of these are advantages: Random access,
cost effectiveness, Increased precision and
accuracy
Hemoglobin, Ferritin, Myoglobin
a. haemolytic anemia
b. Hereditary of Persistence of Fetal
Hemoglobin
c. beta thalassemia
a. Howell Jolly
b. Heinz bodies
c. Basophilic stippling
d. Pappenheimer
Myelocyte
Methemoglobin
Spurr cells
a. Eosinopenia
b. basophilia
c. either
d. neither
a. L1
b. L2
c. L3
D. none
a. PAS
b. SBB
c. Toluidine blue
d. A and C
Type II
Liver
Promegakaryocyte/ Basophilic
megakaryocyte, Early megakaryoblast,
Late megakaryoblast
Thrombin
Thrombospondin, Pf4, (may isa pa
memorize nyo lang ung alpha granules)
Modified Westergren method, Wintrobe
a. Invented by Dr. Quick
b. Fibrinogen dependent
c. Screen for 1,2,5,8
d. when Thromboplastin/cacl2 is added it
will react
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29. Factor that cant be detected through PT Factor 13
and APTT
30. Extreme factor X deficiency shows
a. PT
normal result in:
b. APTT
c. Thrombin time
d. bleeding time
31. Heparin therapy monitoring
APTT
32. 58% haematocrit, 4.5 ml. How much
0.35 ml (1.85 x 10-3)(100-Hematocrit)V
citrate should be removed?
33. Red, needle like crystalline
Auer rods
34. Eosinophil granular contents don’t
a. peroxidase
include
b. myeloperoxidase
c. acid phosphatase
d. b- glucoronidase
35. Precursor of macrophage
Monocyte
36. Increased metamyelocyte, myelocyte
Shift to the left
and promyelocyte
37. Stage in myeloid series found in
a. Segmented neutrophil
peripheral blood
b. Metamyelocyte
c. myelocyte
d. band neutrophil
38. 80 % of lymphocytes are
T cells
39. Indication of 3+ in capillary fragility
Confluent petechiae over the whole arm
and back of the hand, Multiple petechiae
over the whole arm and back of the
hand
40. Estimated platelet count should be
20,000
multiplied to
41. Indications of Sideroblastic Anemia
Increased ferritin, Increased iron, low TIBC,
Increased transferrin saturation
42. Indications of >3 RPI except:
Bone marrow cannot compensate for
the rbc destruction, acute blood loss,
haemolytic anemia, Effective
erythropoiesis
43. End product of Embden Meyerhof
Lactate, Pyruvate
44. Dissociation curve, shift to the right
Decreased affinity, decreased Ph, increased
DPG
45. Inhibitors of erythropoietin, except
a. estrogen
b. insulin
c. interferon
d. Alcohol
e. none
46. Corrected for Protamine sulphate, not
FDP
corrected for toluidine blue.
47. Von willebrand disease exhibits
Prolonged or no aggregation to ristocetin
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48. Conversion of prothrombin to
thrombin
49. Extrinsic Pathway initiation
50. Inherited connective tissue defects
51. Purpura that has emboli stuck to
capillary leading to necrosis.
52. Most versatile cell
53. Megakaryocytic production
54. Extramedullary hematopoesis
55. Erythrocyte stimulation
56. Plasma recalcification time
57. 15 platelets/ OIF x 20,000 x 1M
58. Large, macrophage with foamy
cytoplasm
60. Prolonged APTT, normal PT; Not
corrected by Factor 8 deficient plasma
61. Platelet factors that influence flip-flop
arrangement
62. How to make a thick blood smear
Factor 10a and Factor Va, Factor V and
calcium, Factor VII, Pl
Tissue pathway/ Thromboplastin
Ehlers Danlos, Gronblad Strandberg and
Marfan Syndrome
Purpura fulminans
Totipotent stem cells
6th week
Liver
Tissue hypoxia
For Intrinsic and common pathway
300 x 109 /L
Niemann pick cell
Factor VIII deficiency
PF 3 and anti heparin
Large drop, increased angle, fast, without
pressure
63. 0.45 L/L with 5 percent retics; compute Retics % x (Hct/0.45)=5
for corrected retics
64. Prolonged PT/APTT are caused by
a. prolonged tourniquet
these, except:
b. overfilled anticoagulant
c. icteric
d. all of the above
65. TCT is prolonged because of Lupus
Hexagonal phase
anticoagulant. To check for this, an
expected result should be:
66. Dense granules are numerous than
False
alpha granules
67. Blue cytoplasm, indented nucleus, no
Promegakaryocyte/ Basophilic
nucleoli
megakaryocyte
68. Not true about coagulation
a. lysis is slow because of factor 13
b. Fibrinolysis starts AFTER coagulation
69. Initiating stimulus to coagulation due
Collagen in broken skin
to injury
70. Method used when you move the blood Longitudinal, Crenellation, Battlement
film from side to side in WBC counting
71. Clot retraction starts 30 seconds from
True
blood collection
72. Effect when pressing an incision wound Prolonged bleeding time
73. Phase microscopy does not include
a. Van allen
(Direct platelet count)
b. Sweeny
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74. Anisocytosis is determined by
measurement of?
75. In WHO classification, acute leukemia is
described as:
76.Measures nuclear density
77. All of these are correctly matched,
except?
78.Platelet abnormal function due to factor
3 deficiency
79. Bernard Soulier characteristics
80. Focuses cell one beam at a time
81. Unstable Hemoglobin Disease test
includes except
82. Florence spot test= positive in
fluorescence indicates
83. As cells mature, N:C ratio?
84. Site of Cell development
85. Among Hb variants, what is the most
anodic?
86. Platelet size of 5.6 um
87. Start of alpha granule formation
88. How many days do granulocytes
survive in PB?
89. Erythrocyte 12-17 mm NC ratio 4:1
90. Gp IIb/IIIa is for?
91. 36 wbc, 4 Corners, 1:10 dilution
92. Purple granules in lymphocytes,
granulocytes, and monocytes
93. Complement-mediated lysis for testing
PNH
c. Unoppette
c. Dameshek
RDW
20% in Bone Marrow
Radiofrequency
a. Hemophilia A- Factor 8
b. Hemophilia B: Factor 9
c. Hemophilia C: Factor 11
d. Christmas disease- X-linked recessive
e. None
a. Thrombocytopenia
b. Thrombocythemia
c. Thrombocytopathia
d. Thrombasthenia
a. autosomal dominant
b. gp IIb-IIIa
c. both
d. neither
Hydrodynamic focusing
Isopropanol precipitation test, Heat
denaturation and Heinz body staining
technique, Peptide Analysis, NOTA
Without Pyruvate kinase, NADH is present
Decreases
Yolk sac- Liver- Marrow
Hb Barts
Large platelets, Giant Platelets,
Microthrombocte, Large and Giant
Late stage of megakaryoblast
5-6 days (mali ung tanong dapat
maturation time from stem cells)
Basophilic normoblast/ Basophilic
erythroblast
Aggregation
0.9 x 109/L
Alder-Reilly granules
Sucrose lysis, Donath Landsteiner, Acidified
serum lysis, Sucrose and Acidified (A and
C)
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94. What is not true in TTP
95. Quantitation of A2 is useful in what
disease
96. In normal adult hemoglobin
1. Stain for Hairy Cells
2. Largest in Hematopoietic Cells
3. Reticulocytes correspond with:
4. RDW is a measure of what?
5. Sickle Cell anemia morphology
6. Classic manifestation or sign of
megaloblastic anemia
8. Hemoglobin derivative that does not
reverse to normal hemooglobin
9. Anticoagulant that has diffuse blue color
if using Romanowsky stain
10. Fuelgen (+)
11. What increases in high altitudes?
12. Normal M:E Ratio
13. Order of Draw?
14. Cells with homogenous mass in the
cytoplasm induced by antibodies that can
be seen in special blood tests
15. Reference for RBC
16. Required for diagnosis of
Hemoglobinopathies except:
MAHA, Low coag factors, Low platelets,
Deficiency of ADAMTS-13
Alpha or Beta (not sure which of these)
Alpha-beta chains, Alpha-delta chains
Naphthol, TRAP(Tartrate Resistant
Alkaline Phosphatase)
Megakaryocyte
Increased nucleated RBCs, Increased
polychromasia, increased hypochromasia
Determination of BM activity, Evaluation
of red cell population heterogeneity,
Measures red cell size, Measures red cell
Hb content (RDW measures differences in
size not red cell size directly so I think the
best answer is heterogeneity)
Normo/Normo
Macrocyte with Central pallor, Oval-shaped
with central pallor, (should be Macrocyte
with no central pallor) (answer is
Hypersegmented Neutrophils (this is
the only viable option), Leukopenia
Hemoglobin, Oxyhemoglobin,
Methemoglobin, Sulfhemoglobin
Heparin, Oxalate, EDTA?(this answer is
only based upon my judgment), Citrate
Howell Jolly bodies, Heinz bodies,
Reticulocyte, Basket cells
Hct, Red Cells, Hgb, WBC (Im sure about
Hematocrit and Red Cells. Not sure about
Hgb)
2:1-4:1 (3:1 can be the answer)
Bacte(yellow) > Citrate (Blue) > Red (no
anticoagulant) > Heparin(Green) > EDTA
(Purple) > Sodium Fluoride (Gray)
LE Cell, Pegler-huet anomaly, Macrophage,
Histiocytes
Neutrophil, Small Lymphocyte, Large
Lymphocyte, Basophil
Dithionite Tube test, Hb Electrophoresis,
Red Cell Morphology evaluation,
Leukocyte differential
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17. EPO is increased in Polycythemia vera
18. Reference range of RBCs in Cu/mm
19. Recommended microscope for Manual
Cell Count
20. Christmas disease is a deficiency of
what factor
21. How many attempts in phlebotomy is
allowed?
22. Nuclear remnant of lymphocyte
23. Solution to lyse RBCs
24. Neutrophil function test
25. Anticoagulant for fibrinogen
26. Modified Drabkin’s reagent
27. Antibody in PCH
28. Niemann-Pick disease inclusion
29. ____ Nucleated RBC per 100 WBC found
in differential count that indicates recount
30. Precision in BT
31. Number of heme per globin chain
32. Giant metamyelocyte are seen in:?
33. Youngest cell of neutrophil
34. Neutrophils pass through blood vessels
35. Most common drug that interferes with
platelet funcion
36. Disorder of platelet functions. Alpha
granules are deficient
37. Morphology of Megaloblastic anemia
38. Mistaken for pappenheimer bodies
False
150,000-200,000 cu/mm, 4000-6000
cu/mm, 4-6million cu/mm
Phase Contrast, Light Microscope?,
Darkfield microscopy, Electron microscope
Factor IX
2,3,4,5
Necrotic cell, Burr Cell, Smudge cell,
Phagocytic cell, Basket Cell
Dacie’s fluid, Turk’s fluid, Gower’s fluid,
0.85% NaCl (definitely not 0.85% NaCl)
Nitroblue tetrazolium dye test, Kleihauer
Betke
Citrate, EDTA, Heparin
Potassium ferricyanide, potassium
cyanide, Distilled Water, Detergent
IgG, IgM
Sphingomyelin, Beta-glucosidase, Betagalactosidase, Alpha-glucosidase
More than 5, More than 4, More than 3,
More than 2, More than 1
Depth of wound, Size of wound,
standardization AOTA
1,2,3,4
Pernicious Anemia, Megaloblastic
anemia(kasi according to Hema1 pg. 69.
Giant neutrophils and bands are
common in megaloblastic anemia),
Myeloblast
Diapedesis
Aspirin
Gray Platelet syndrome, Bernard Soulier,
Gronblad Strandberg. ET
Macro/Hypo
Pappenheimer, Heinz, Retics?
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BLOOD BANKING/IMMUNO-SERO
IMMUNO-DK
Lw Ag except ( ABO, X, LW,)
NOTA
Characteristics of cold red cell autoAb
Thermal amplitude
Diff. O and BOMBAY
Reverse
HBv Ag
HBsAg
Grp A1 cells from A2 cells
Dolichus bifluorus
Live attenuated
pasteur
Hb determination , mass blood donation
Copper sulfate
Most associated of mortality
TRALI
Plts prepared from whole blood
Random
Ovarian adenocarcinoma
CA125
Prevent MAC
Protein S
TTp
P24
Prepare using light spin except
Platelets
Least able to cross placenta
IgG2
Fctors not found in FFP
Factor 5
Deferred to donate
Transmitted HIV, HBV, HCV
Engulf material and enzymatic granules
Phagolysosome
1 year deferral
Malaria
Agglu of soluble Ag and Ab
Precipitation
Ankylosing spondylitis
HLA B27
Staining pattern characteristic of drug
Peripheral
induce lupus
C3 def.
RID
False negative of Ab
Prozone
10 am stored blood @ 20-24C
4 am
Duplication of HIV test
Cleared for donation
Complement dependent cyotoxicity
1 bag RBC
1g/dl Hb
Measurement of total IgE
Radioimmunoabsorbent
Grp A
Blue
Basophil in tissue
Mast cell
Ag-ab rxn results in aggre of particles
Agglutination
EBV heterophil Ab absorption with
EBV Ab
Secondary response to same foreign
anamnestic
Fetal Rh positive RBC 30 detected in Rh
3
negative woman
First line of transfusion therapy
Cystalloid sol’n
Pertussis coughing with whooping
Paroxysmal
IgA def
Anaphylactic transfusion
Phagocyte during opsonization
Fc
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RPR cardiolipin
Lymphoid stem cell except
Hepa A infection
Common anticoag in apheresis
Phagocyte except
Lyme dse stage detection
Infect RBC by bite of deer tick
Positive direct coomb’s test
Confirmatory to HIV
Least transmitted thru blood transfusion
True about Rh
Purpose of major cross match
Cannot induce immune response
Monoclonal IgG
Gives off light in chem rxn
Complement inactivation
Require pre transfusion
Adjuvant stimulates positive cells except
Ab most present in SLE
Most specific for active SLE infection
In pedigree analysis
Stool of acutely ill during early days
Complement system requires Ag-Ab
RBC in traditional heterophil Ab
Vaccine induces cell mediated response
Indirect fuorescent immune assay
Prevent transfusion associated graft vs
host dse
Acute phase of heap, onset of uremic state
Receive 10 units of plts , ff transfusion
count is 2000 only
Anti-SD(a) stongly suspected
Not true in LEd Ag
AABB std require Ab detection testing
include
Weil Felix
Charcoal
NOTA
Anti-HAV IgM
CPDA
Mast cell
Icteric stage
AOTA
Westernblot
Syphilis
45 blood type, more complex than ABO
Letter A ..hahaha
Hapten
Single clone of B-cells
Acridium ester
Heating 56C and EDTA
ABO, Rh, Ab screening, Crossmatch
Nuclear Ag
peripheral
Propositus
Classical pathway
Live, attenuated
Hetero
Gamma irradiation
Mixed filled refractile
AHG testing
Proteus vulgaris or Rickettsiae
IMMUNO DK
EBV heterophil ab absorb
P.127
Incr. In hemolytic dse? (newborn)
IgG
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IgM & IgG
acute
TTP component of blood
platelets
RID endpoint
Macini
Confirmatory HIV
PCR, Westernblot
Indirect
Hetero or homo
Pwede mag donate, Hct and Hgb normal.count
Hct= (F= 36-46; M= 41-57) Hb= (F= 1216; M= 13.5-17.5)
Donor smp must stores how long in transfusion
service
1 week
Tumor marker is for...function
P.91
AABB, ab detection also involve?
Autologous Hct
>11g/dL hb ; hct 33%
Not true in modified WB
Higher serum dilution; no hemolysis
End point of ASO
Highest dilution without hemolysis
(todd units)
Presence of ASO in antistreptolysin O
P. 105,106
Malaria deferral
1yr (travel) 3yr (stayed for >5yrs)
Immunoglubulin in B lymp
IgD and IgM
FFP Factors not found in transfusion
Less nicks in transfusion kasi during 1990s
nadetect yung ab
H
Order of collection
Interview, collection of blood,
screening test, bleeding
Flourescent stain
Purpose of washing three times before adding
AHG?
Remove free serum globulins
33 y/o female, RH neg, 2nd pregnancy at 17 weeks.
Recommendation?
a. Repeat titer after 1 month, b. Follow
titer until >64, c. administer Rh
immune globulin, d. Perform middle
cranial artery doppler, e. Perform…
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Most common type of Duffy?
FyA
Autoimmune antibody reacts with Fc & IgG
molecule and immunoglobulin complex?
Parasite from deer tick
RF
Not an enhancement medium for Coomb’s testing?
NSS
Decrease or lack of MHC 1 activates?
Cytotoxic T cell, Helper T Cell, NK Cell,
Direct AHG useful for the following, EXCEPT?
HDN, Hepatitis, Hemolytic Anemia,
Transfusion reaction?
Group A, Lea, non-secretor?
red cell: A, Lea ; secretion: Lea ??
Todd unit
ASO
Ulex Europas
Anti H
Lyme Disease best stage to measure serologic tests
Icteric???hndi namin alm ang choices.
Donor who received blood in the last 12 months;
reason why cannot donate
Two blood population
Competent phagocyte
Denritic cell
Accident component to transfuse. 16% Hematocrit.
123,000 platelets
Packed rbc
A1B mother A20 father
phenotype not possible
Germ cell tumor marker
A1 Fetoprotein and HcG?
SLE antibody type ANA
Nuclear ab
Most serious transfusion reaction
ABO/HLA
B cell induced by IL4 and IL5
redundancy
Solid Organ that doesn’t need blood component
liver
Leptospirosis
Rat and cattle
Most abundant Ab in plasma
IgG
F. tularences
PCR for?
Amplify DNA
C3 def. test
AOTA
Prevent Membrane attack complex
Protien S
Blood component to treat Hypofibrinogenemia
cryoprecipitate
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Rh hndi namin alm yung tanong! Tsaka
choices.
L.w expression
IMMUNO DK
RPR component
charcoal
Negative B cell selection
Bone marrow
2 times auto. donation
Activates T cell, EXCEPT:
Pg. 37
Recipient IgA
Afebril transfu rxn?
Lypho line, EXCEPT
Nk, t cell, B, none?
Best detect cell immune mediated
T cell
Ovarian cancer tumor marker
CA 125
dombrock
014
Marker HBV
HbsAg
Wiel felix
rickketsia
Cold red cell hemolysis
Titer, thermal...?
Components of informtion system except:
Hardware, software, human,
validity
IgA neg. Person PNH
Wash RBC
Polyspecific AHG reagent contains
Anti-IgG and anti-C3d
Which step in PCR rxn allows the rxn to be cooled
at 550C?
Annealing
Donor starts to hyperventilate
Rebreathe into paper bag
Unexpected antibodies reacts best at 370C are
Usually natural occuring antibodies
Ankylosing spondilitis is highly associated with
inheritance of which MCH gene?
HLA-B27
Not cause hemolytic disease of newborn
P
A1B mother and A2O father could not produce
phenotype
A2O
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Germ cell tumor
AFP and B-hcg
Antibody present in SLE is directed against
Nuclear antigen
Most severe blood transfusion reactions is
associated with
ABO incompatibility
Todd unit for reporting results of
ASO titration test
Hypersensitivity of RA and SLE
Type II
Ulex europeus
Anti-H
Most competent phagocyte
Dendritic cell
B lymp to release IgA
TNF beta
Exaggerated rxn
hypersensitiviy
IgM in CMV
Primary & reinfection
Rbc will not agglutinate w/ anti-A,B
O
Not asso w/ HDFN
Dombrock and Cromer
A blood Group
N - acetyl - galactosyltransferase
Purple tinge in blood bag
Bacterial contamination
D 1. Robert Koch Discovered
D 2. Humoral Immunity
D 3. Innate Defense Components
D 4. Innate defense are against organisms
that are rich in
5. Outcome of Donor Screening Process
Delayed Hypersensitivity
Emil Von Behring/Elie Metchnikoff
All except Plasma cells and Antibodies
(1,2,3,4,5,6,7)
Mannose, Peptidoglycan, Acid-fast
Less harm to donor, Less harm to recipient,
Either, Neither
6. WB on Sept 20, 2016 in CPD + AS3 expiry Nov 1, 2016 (42 days)
date
7. Platelet time and storage
5 days, 20-24C, Agitator
8. RBC Lesion that is increased
Lactic Acid, Glucose, ATP,
B 9. Limitation of Type and Screen test
No crossmatch, For Surgery pxs only, Does
not reduce the cost,
B 10. Increase the conc by decreasing
PEG, LISS, Albumin, Polybrene
water molecules surrounding RBCs
B. 11. 70% of Autoimmune rxn and are
Warm-reactive, Cold reactive, Drughighly connected to RH
induced
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B 12. Most important test for diagnosis of
HDN
B 13. In gel testing, blood cell button on the
bottom of the microtube
B 14. Pedigree: pointing arrow
B 15. Hemophilia A:
B 16. Donor Screening includes:
B 17. Who cannot donate after a year
B 18. Autologous Donor
B 19. Accepted donor?
B 20. In MHC class II, every individual
inherits a set of which genes
B 21. Primary mode of transmission of
Treponema Pallidum
B 22. Which stage of Syphilis is
mismatched?
B 23. Which stage infection of Bordetella
pertussis is characterized by strong cough
with whooping sound
B 24. Weil’s disease is caused by
B 25. Which hepavirus infection has a
longer incubation period?
A 26. The following are true about noncompetitive ELISA, except
A 27. PCR cooled at 55C
A 28. Mother R1R2, Father R1r, what is not
possible
A 29. Anti-H lectin
D 30. Recent german Measles infection
what IG
D 31. Fungi infection is enhanced with
D 32. AIDS-related Complex is associated
with
D 33. Detection of Capsular Polysaccharide
Antigen
D 34. Best way (correlates with) of getting
Toxoplasma
DAT using IgG, IAT using IgG, Kleihauer
Betke, Anti C3 antiglobulin
Negative, 2+, 4+, Mixedfield
Propositus
X Linked Recessive
History, Physical Examination, Sero
Testing
Needle prick, Allogeneic bllod, Tattoos,
Viral hepa after 11th birthday
Mother who gave birth 5 months ago,
Athlete 40bps/minute, Nagpavaccinate
for measles 2 weeks ago, Malaria 3 years
ago, Palawan last 5 months
HLA-DP, DQ, DR
Sexual contact,
1- Chancre, 2- Rash, LatentGranulomatous lesion, 3- Tabes Dorsalis
Paroxysmal
L. interrogans (Leptospira)
A, B, Hepa C, D
The antigens compete with enzyme
labeled antigens
Annealing
DCE/DcE (Bawal may dalawang big E kasi
sa mother lang may Big E)
Ulex Europaeus
IgG, IgM
Immunodeficiency, Broadwave
spectrum antibiotic, Organ Transplants
(Lahat sila)
Slow Decrease of CD4+ cells
Aspergillosis, Coccidiodoycosis,
Cryptococcus
Ingestion of Cyst, Fecal oral
contamination of infected cat,
Transmission from mother (medyo di ko
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D 35. Detect antibodies of Toxoplasma
(Most sensitive and specific)
36. Antibodies enhanced by enzyme
treatment
37. Associated most with Delayed HTR
D 38. Recipient serum + RBC from
Bloodbag
D 39. Check cells added Negative result
with no agglutination, what to do?
B 40. Marker of Hepa B- Core window
B 41. Surface Marker for Activated B cells
B 42. Composition of TCR Complex
B 43. First antigen after exposure
B 44. Process that uses cell-mediated
immunity
C 45. Cause of false positive AHG
C 46. RBC diluent not causing distortion
C 47. Packed rbc is transfused within:
D 48. Up to how many days will an
outdated RBC pack still be used after
rejuvenation
D 49. How to prepare platelet concentrate
D 50. Post-storage centrifugation to make
leuko-reduced blood, how reduced are the
WBCs
51. Aliquot is how many
52. Thawed cryoprecipitate expiration
53. 14 year old IgA deficient w/ previous
serious reaction
54. Cryoprecipitate is used for the
following except:
55. Multiple factor deficiencies and
hemorrhage mag surgery
56. Associated with drug users that occur
with HBV
57. Blood component most associated with
septic reaction
B 58. All about MHC II except
sure to ha kasi lahat naman sila pwede
magtransmit)
Sabin-Feldman Dye Test
Rh, Lewis, Kidd (pati P, ABO, at I)
Anti-JKa
Major Crossmatch
Test result is invalid and test must be
repeated
IgM Anti-HbC
CD25
TCR(ab), CD3, $ chain
IgM
(As long as nasa loob ng cell ung
bacteria/virus)
Dirty Glassware, Low Saline, Cell
Suspension too heavy, Inadequate washing
Isotonic saline
4 hours
3 days
Centrifuge at 3200, 2-3minutes then
3600 for 5 minutes (basta light muna
bago heavy spin)
Less than 5x10^8
10-25 mL
6 hours (if pooled maging 4 hours)
pRBC, deglycerolized (if may washed RBCs
sa choices then ayun ung sagot, meron ba?)
Volume replacement
Fresh Frozen Plasma
HDV
Red cell, granulocyte, Platelets
All nucleated, Alpha and beta
microglobulin, Cytotoxic T cells, AOTA
kasi MALI LAHAT
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B 59. Mechanism of evasion of parasites
against antibodies
B 60. Exophthalmos or Bulging Eyes
B 61. 35 yr old asked to test for ANA.
Rashes, sensitive to sunlight, seizures
B 62. CREST anung hindi kasama
B 63. Tumor confined at primary site
during lifespan
D 64. Germ Cell Tumor
65. Reason why leukoreduced blood
component is given to a patient who had a
stem cell transplant
66. Most severe hemolytic reactions
associated with:
B 67. Most abundant complement
B 68. Do microbes without antibody
antigen complex activate complement?
B 69. Role of IL2 in Tcell activation
B 70. What will happen if APC that goes to
T cell does not express costimulator
B 71. MHC I is presented to?
72. There is an age limit for autologous
73. To determine hemoglobin, reagent
required for mass blood donation
74. Bloodbank information system
component except:
75. Outcome of Donor Screening: NAULIT
KO huhu
76. Chills, Hypotension, increased
Respiratory Distress
77. Result ng both Anti A1 and B if no
agglutination ang cells sa backtyping
78. All of these are true about Anti-AB
except:
79. HIV I and II sa screening ay slightly
above cutoff, what to do?
80. IAT vs DAT anu difference
Shedding of Antigen
Graves Disease
RA, RF, Confirm SLE, Pernicious Anemia
Calcinosis cutis. Raynauds phenomenon,
Esophageal dysmotility, Spondylitis,
Telangiectasia
Benign
AFP & B-HCG
To prevent graft v host disease
ABO Incompatibility, HLA incompatibility
C3
Yes (Panu ung pag question dito?)
Autocrine
No T cell activation, Cannot attach
integrin
Cytotoxic T cells (CD8)
False
Copper sulfate
Validation
TRALI
Walang choices na binigay pero ganto:
Since no agglutination sa backtyping, wala
syang Px AntiA at Px AntiB so ang result ng
reagent Anti A1 at Reagent anti-B ay
negative dapat
You can make Anti-AB by mixing
monoclonal Anti-A and Anti-B
Repeat in duplicate
Walang choices . Basta ung Invitro ung
IAT tapos it determines phenotypes, weak
D, chuchu.
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81. In a pre-B cell receptor, mu chain is
combined with
82. IgG subclass that can bind complement
the most
83. Interaction between single antigen and
antibody
84. Not an important role of Monocyte
85. In the absence of T cell help, this elicits
a very weak response
86. Neutralize Anti-p1
87. Group B nonsecretor w/ Lele genes will
have what antigens in the Saliva
88. Most potent phagocyte
89. Not a prostate CA marker
90. Type III Hypersensitivity is against
91. Western blot of HIV detects
92. RID that measures the diameter of the
ring after it has diffused completely
93. Detects specific antigen
94. AntiA 4+, AntiB 2+, A1 (0), B 4+, Check
0, Autocontrol 0
95. We do antibody screening due to:
96. What chemical will increase shelf life
from 21 to 35 days
97. Not associated with HDFN
98. Platelets should be stored in what if not
tested immediately. (Di ko sure if naulit
ung tanong or naulit ko lang talaga)
Surrogate Light Chain
IgG3
Affinity, Avidity
NOTA ata? Haha Walang binigay na choices
Proteins, Lipids, Nonprotein antigens,
Lipopoloysaccharide
Hydatid Cyst Fluid
Le(a) ONLY
Dendritic Cell
CEA, ALP
Soluble Antigens, Particulate antigens,
Pollens
P24 and gp120 (may iba pa actually gp41
and gp160)
Mancini (kapag may time Mckelvey)
Agglutination inhibition, Direct agglu,
passive agglu, hemagglu
Acquired B antigen
Clinically significant unexpected antibodies
Adenine, Dextrose
Dombrock and Cromer (basta ung may
dombrock)
20-24C. Agitator
All in All 96 Viable questions ung nanjan
1. Two veins used for Apheresis
2. Extracorporeal Blood Volume should not
exceed __% of the donor’s estimated total
volume
3. Amorph in Rh Blood Group
4. D+ C+ c+ E+ e+
5. Hemolytic reaction is under what type of
Hypersensitivity?
CFC, IFC
15%, 20%, 40%, 50% (Bloodbank page
343)
C,c,D,d
RzRo, R(o)r(y), R(z),r(y) R’r” R2r ? (Either
RzRo or Rory can be an answer kasi.)
Type 2 Hypersensitivity
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6. Uses strains of bacteria, the reaction is
agglutination
7. Antigen that doesn’t induce an immune
response
8. In IFA, which pattern is most specific for
SLE
9. In Competitive ELISA, amount of antigen
is _____ to color conc.
10. Largest dilution that gives/produces
desired result
11.IFA agglutination for SLE, active SLE
pattern?
12. Globulin in inflammatory disease
13. Without congenital form
14. Prepared from whole blood units
15. Not tested in donation
16. Positive compatibility except
17. Suspension of LW antigen except:
18. In the bag, what is the minimal
precaution?
19. Donor requirements except:
20. Tumor marker most widely used in
gastrointestinal CA
21. Serologic marker for HBV phase,
window period?
22. O is universal donor because
23. AB is universal receiver because
24. Asthma is what type of Hypersensitivity
25. Biologically activity portion of Ig?
26. 1st marker for HBV for acute?
29. O donor to B recipient
30. Bombay patient reaction to Anti-H
31. Anti-B Lectin
32. Sugar of B group
33. Direct coombs test sample
34. Antibody that is secondary booster
35. Non-treponemal test
36. SLE Test
37. First to respond in a localize
inflammation
Widal, ASO, RPR, Paul-bunnell
Hapten
Speckled, Peripheral, Homogenous?
Inversely proportional, Directly
proportional
Titer, Zone of equivalence, Prozone (
Homogenous, Peripheral, Nucleolar,
Speckled
ASO, Rheumatoid Factor, B-HcG
Syphilis, Pertussis
Random donor
CMV, Malaria, Hepa C, HIV
Hemagglutination and Hemolysis
ABO, X Gene, LW, AOTA, NOTA(Supposed
to be Rh. Based only upon my judgment.
ABO is not an answer kasi eh)
Write mailing address of patient, Initial
and date of collection?
Physically healthy, Fasting state, 17 and
above
CEA
It has no antigens?
It has no antibodies?
Type I
F(ab), F(ab)2? Fc
HBsAg
Incompatible minor crossmatch
Negative
Bandereia Simplicifolia
D-Galactose
Recipient/px serum
IgG
Reagin, Complement, ANA
ANA, Compement
T lymphocyte, lymphocyte, neutrophil
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38. Response to foreign antigenic stimulus
(type of immunity)
39. Associated with autoimmune specific
against Fc portion of IgG
Adaptive Immunity
RF?
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HISTOPATHOLOGY/MEDTECH LAWS AND BIOETHICS
HISTOPTHOLOGY AND MTLB
Best dehydrating process
Ascending grades of alcohol
Glycogen fixative
Rossman’s
Formaldehyde pH
Neutral
Study of cell, shed, scraped off
Exfoliative cytology
Complete non appearnce
Agenesia
Freez substitution method
Osmic and rossman (both)
Gross nicks
Honing
Formalin pigment
AOTA
Knife for ultra thin
Plate glass
Completeness of dehydration;monitor drop DMP
of temperature
Autopsy adult cadaver
y-shape
Specific subjects to be given
Section 17
Transfer of DK lab
Yes, renew LTO
Milky or cloudy
Incomplete dehydration
Increasing conc. Of alcohol
Dehydration
Third amendment of RA 5527
June 11, 1978
Suspension or revocation of COR
AOTA
Registered medtech supervised how many 5
med. technicians
Substance added to adhesive to prevent
Thymol crystal
mold
Dangerous drug act 2002
RA 9165
Alteration in arterial flow
Active hyperemia
Brittleness and hardness
AOTA
To restore natural color
70% alcohol
Fixative for embedding GMA and ultra thin
Fifth step
embedding
Who can practice medtech in phil.
AOTA
Infiltrating substistute for paraffin except
NOTA
Head of blood bank
Clinical pathologist
Board exam issued license
Yes, claim when 21
Not a hereditary trait
Behavior
Average computation to guys
75.83%
Most important item besides px name
Specimen
Composition of MT board
Chairman and 2 legal officers
Fixation time of pap smear
60 mins
Register medtech shall conduct a
Reliability, honesty, intergrity
reputation of
To toe heal direction
stropping
Retard tissue fixation except
NOTA
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Monitor performance school, publish result
of board exam
How many witnesses for autopsy
Glacial acetic acid
Sliding microtome
Purulent exudates
Purpose of fixation except
Reagent used as fixative and dehydrating
agent
Open biopsy
Prolong decalcification
Exudates Large amount of mucus
Fibrous connective tissue
Wax for hollow and dense
Presence of ferning except
Most common decalcifying agent
Ammendments of 5527 except
GC/MS
Nissl granules
Approved refresher course
Cause of lost and inactivation of enzyme
New born screening act
Wartons jelly
justice
symptoms
Fixative for cytological smear
Stainig aftet removal
Crumbling and tearing
Detectable by observer
HIV/ AIDs
Raised surface lesion on the skin
Stain for cytological studies
Contains rubber
Power of PRC except
Whole eye section
RA 7719
Passage through birth canal
Manifestation of dse subjective
Metal parts
Medtech attending -------Newborn screening of 2004
Injections of dye to living body
PRC
2
Destroy mitochondria and golgi apparatus
Cutting celloidin and large paraffin
materials
PMNs
Solubilization of tissues
Acetone
Excisional
AOTA
Catarrhal
Both
celloidin
High progesterone level
Acid
NOTA
Drug testing
Toluidine blue
Council
Wrong choice of fixative fluid
RA9288
Connective tissue
Letter D guys ..hahaha (tight ng px maka
receive ng tamang tx)
Headache
95% ethyl alcohol
Supravital
AOTA
Sign
5-7
Scalpel
Pap smear
Tissue mat
Inspects school
Dry
AO#9 s.1995
Traumatic lesion
Symptoms
Xylol
1/hr
April 7, 2004
Intravital
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Microtome for paraffin embedded
Temp of floating out bath
Celloidin usually takes
Step after sectioning and before staining
Freeze substitution
Non hospital based category
Angle between cutting facet
NRL HIV
Current candidate which will disqualify
Nuclear fixative except
Deficiency in nicotine assay or vit. B
complex
Rotary
45-50C
1 day
Deparaffinization
Quenching and desiccation
Donor selection
Clearance
San Lazaro
2,3,5
(Reguard) 4 is the only aswer
Pellagra
Histopath MTLB
Changing decalcifying sol’n
5527
June 21,1969
Simplest and common rgt
5th step
embedding
PRC power
Heel to Toe
honing
Wax for wet
Prevent mold formation
Gelatin, glycerol..?
Purpose of fixation
Celloidin?days.
To remove the color
Xylol, alcohol...?
Metal wash
Oil, water, xylol?
Leather strop, EXCEPT:
Size of tissue in fixative
Observe and detectable
Wag gamitin na fixative for decalcify
For eye
Dry celloidin
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Produce of licensure of exam and newspaper
Assist medtech
Boat shape; curl edges
Paraffin ebedden in rotary
biconcave
paraffin
Produce of microtome
Ultratin
0.5
Clinical laboratory should be monitored every?
- (30 days?)
- Staining for bacteria. G/S
Which of the following is not a Nuclear fixation
(madami ba yun? Memorize na lang, nakalimutan
ko yung choices ; ; sorry na)
Justice refers to…?
Year
Step after sectioning before staining?
Deparaffinization
Oath to be taken after passing the board exam?
Prof.??
RA 7719, yung rules and regulation
AO 27 s. 2007?
Staining for Nissl granules?
Function of Gilson’s mixture
dry
How many days/years a laboratory store a urine
specimen tested positive for screening drug test?
15
Proper order of autopsy consent.
EDTA
Spouse, children, father, mother
Chelating
HIV Core Council # of members
5-7
Reference Laboratory
RITM
Phospholip sectioning?
council constitution
National Organization of Medtechs
PASMETH/PAMET
Freeze substitution
Needle punch blah blah, incision/excision?
incision
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Underage Medical Technologist claiming license
procedure
Yes, only when 21
Lipid and fat microtome?
Freezing
Gangrene invasion without phagocytosis
Dry
Spirochete stain
Rubber embessing
Basta Mat. Letter C daw
Removing of burr
stropping
ferning
High progesterone
1. Most rapid fixative
2. Most used fixative for Routine biopsy
3. Most used fixative for blood smears
4. Most widely used fixative for exfoliative
cytology.
5. Most rapid clearing agent
6. Microtome for plastic embedded tissue.
7. In clearing, what does the presence of
cloudy medium means?
8. To prevent insufficient dehydration,
what should you do?
9. Autopsy is required for the following,
except?
10. Example of metabolic disturbance,
except?
11. Ratio of fixative to tissue volume
12. Best dehydrating agent
13. Most rapid dehydrating agent
14. Temperature of water bath
15. Embedding medium for GMA and
ultrathin microscopy
16. Acetone is used for?
17. Cryostat and environment should be of
the same temperature
18. A machine used to slice thin tissues for
microscopic examination
Carnoy’s fluid
Formalin, Alcohol, Acetone
Alcohol (methyl alcohol)
95% Alcohol
Xylene
Ultra thin microtome, Rotary, Rocking
Insufficient dehydration
Change absolute alcohol, Change
Acetone, Change fixative
a. Approval of nearest kin/relative
b.2 witnesses
c. procedure of autopsy
d. court order
a. edema
b. gout
c. freckles
d. Uric Acid
10-20x
Ethyl Alcohol
Acetone
45-50
Plastic?
Brain
True
Microtome
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19. Increase in size due to increase in
number of cell
20. Reversible change of an adult cell to
another adult cell
21. Most commonly used agent for
decalcification
22. Removal of organ using scalpel
23. Permanent mounting medium, except?
24. Derivative of paraffin and DMSO
25. Cut done in autopsy of adults
26. Needle, shave, punch biopsy are
examples of
27.Placing in a second fixative
28. Rabies, brain tissue fixative
29. Fixative for embryos
30. Stain that is in need of background for
contrast
31. Best embedding media
32. Not used in leather stropes
33. Removal of gross nicks
34. When was the Medtech Act of 1969
approved?
35. Oath taking will be done in the
presence of?
36. Appoints the 3-man commission of PRC
37. Components of the 3 man commission
of PRC
38. Functions of the board
39. The Board consists of:
40. Assists RMT and pathologist
41. Dangerous drugs Act
42. Chairman of Council of Medical
Technology
43. Most commonly used microtome in the
lab
44. Did not reach full maturity
45. Which of these is a developmental
defect?
Hyperplasia
Metaplasia
Nitric Acid
Excisional
a. Clarite X
b. XAM
c. Canada balsam
d. Von Apathy
Paraplast
Y-cut
Incisional
Postmordanting
Acetone
Bouin’s solution
Counter staining
Paraffin
Mineral oil
Honing
June 21, 1969
a. judge
b. president
3. member of the board
4. PAMET president
President of the Philippines
1 full time chairman and 2 full time
commissioners, 1 full time 2 part time, 1
full time 1 part 1 associate
Refer to book
2 RMTs, 1 pathologist, 2 RMTs 1
physician,
Med lab technician
RA 9165
Chairman of Higher Education
Rotary microtome
Hypoplasia
a. hyperplasia
b. atrophy
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c. hypoplasia
46. Minimum Required Course section
6
47. Who suspends, revokes and issues COR The Board
48. Not considered a work of a medtech
a. blood bank techniques
b. preparation of reagents
c. ensures quality control
d. Process samples
49. Exudates with large amounts of mucus Catarrhal
50. Fixative for glycogen
Rossman’s
51. Fixative for color photography
Mercuric chloride
52. Medtechs who can practice without
a. medtech @ US Army base
COR
b. Foreigner
c. Physician
d. AOTA
53. Revision of RA 5527
a. 1539
b. 498
c. 6138
d AOTA
54. Which of these is a cause of low arterial
blood supply to the tissue/ organ
55. Xylol can be used to remove what?
56. Effect of static energy to the tissue
57. Staining method in which the stain is
injected
58. Fixative for peripheral blood smear
59. Staining that requires a mediator?
60. Female exfoliative cytology stain
61. An example of symptom is?
62. Well defined and characteristic
structural change produced by disease in
tissues
63. Blood bank Act after 1517
64. How many medtechs should be in the
lab which performs 800 tests/ month
65. Third amendment of RA 5527
66. It is not a practice of a medical
technologist.
Deep-tissue laceration, exercise,
pregnancy, leg plaster cast
a. remove paraffin
b. remove alcohol
c. remove stain
d. make tissue transparent
Adhere to the knife
Intravital staining
Alcohol
Indirect staining
Papanicolau
a. jaundice
b. fever
c. headache
d. swelling
Lesion
7719
1,2 (di ko sure)
June 11, 1978
Treat and diagnose
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67. Who started the medtech curriculum in
the Philippines
68. Drugs that is used for blah blah blah
which is abused
69. How many years is a lab’s license valid?
Lto
70. The council is in charge of giving
refresher course to those that failed..
71. Reasons for revocation/ suspension of
COR of medtech
72. Revocation of license could be achieved
if there’s a?
73. Dangerous Drugs Act of 2002
74. A medtech is not allowed to
75. Organization in overseeing AIDS/ HIV
prevention and control in Philippines
76. Puncture, rotate and use needle to get
77. Most rapid decalcifying agent
78. Very excellent dehydrating agent but is
toxic
1. Disease manifestation correlating the
underlying structural and functional
changes
2. Most important factor in susceptibility
and resistance to disease
3. Enters while the disease is already
present. Changes the course of the disease
4. Detectable by Observer
5. Congenital disorders is associated to the
ff. except:
6. Component of a cell that digests foreign
substances
7. Alcohol
8. Microtome that uses biconcave in cutting
paraffin embedded tissue
9. Papsmear minimum fixative time
10. Kardesewitch’s and Lillie’s method
removes what?
11. Reagent that reacts with water to form
methanol and acetone
Mrs. Willa Hedricks
CLINICAL CHEM TO
1 year
3 times
a. negligence
b. fraudulent lab report
c. malpractice
d. AOTA
Unanimous decision of the board
RA 9165
Head a clinical lab
PNAC
Needle biopsy, FNAB, Punch
Nitric agent
Dioxane
Pathogenesis, Etiology, Pathophysiology,
Morphological Changes (may gandang
choice ba? Kasi eto tamang sagot)
Environment, Nutrition, Emotion,
Constitution
Fulminating, Complication, Intercurrent
Signs, Symptoms
Present at birth, No genetic basis, Confined
in the period of gestation, NOTA
Lysosomes,
Increasing concentration
Rotary Microtome
30 mins, 45 mins, 60mins, 2 hours
(histopath page 148)
Formalin Pigments, Mercury, Excess
picric acid, Chromates
Benzene, DMP(Dimethoxypropane)
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12. Tumor cell diagnostic features except:
13. Crumbling and tearing of tissue during
sectioning is due to:
14. Fixative used for decalcification except:
15. Knife for ultrathin
16. What is not true about atrophy
17. Sharpening the knife
18. What automated machine is used in
Histopath for clearing, dehydrating
19. Vaginal cell, acidophilic, dark, pyknotic
20. Manifestation of disease that is
subjective
21. Abnormalities in the birth canal
22. Xylol, except:
23. Tumor cell of Epithelial origin
24. Pap smears used in the following
except:
25. Increase amount of blood due to
alteration of arterial blood flow
26. Type of fixative that specializes in the
chemical constituent of the cell
27. State of collapse after injury resulting
to insufficient oxygen distributed to tissue
28. Escape of RBC from the vascular system
29. Refractive index of mounting medium
30. The process of removing nicks
31. Used for hormonal studies:
32. Class 1 in Pap’s ID of vaginal/cervical
smears
Decreased cell size, abnormal
vacuolation, hyperchromaticity,
increased cell number,
Dull knife, Paraffin soft and warm,
incomplete dehydration, incomplete
infiltration, AOTA
Helly’s fluid, Zenker fluid, Buffered
formalin, NOTA
Plate glass, Biconcave, Plain Concave,
Plain wedge
Increased total number of cells, increased
total number of size, developmental defect,
AOTA(all of these kasi is not true)
Toe-to-heel leather strop, Toe-to-heel
stone, Heel-to-toe leather strop, heel-to-toe
stone
Autotechnicon, Microtome, Leica
Superficial, Intermediate, Parabasal, Basal
Symptoms
Congenital, Familial, Traumatic Lesion,
Hereditary,
Dehydration, Dealcoholization,
Deparaffinization, Making tissue
transparent (PLEASE TAKE NOTE IN THE
HISTOPATH 1ST TAKE THAT XYLOL MAKES
TISSUES TRANSPARENT) histopath page
77
Lymphoma, Sarcoma, Carcinoma
Diagnosis of Cancer, Hormonal studies,
Genetic sex studies, cytologic diagnosis of
anemia
Active hyperemia, Localized passive,
General passive
Histochemical, Cytoplasmic,
Microanatomical
Shock, hyperemia, ischemia,inflammation
Edema, Hemorrhage
1.518
Honing
Cervical smear, Vaginal smear
No atypical cells seen
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33. If one kidney is removed, the other
kidney compensates. What is that?
34. Decreased O2 supply to organs
35. Thickness of paraffin
36. Adhesion to paraffin except:
37. Which is not a temporary mounting
medium?
38. Phospholipid Stain
39. Fixative used for ultra thin sectioning
40. Dehydrating and clearing agent
41. Brittleness and Hardening
42. Flatten tissue except:
43. Study of body fluids:
44. Fix with gauze/cotton
Compensatory hypertrophy,
Compensatory hyperplasia
Ischemia, Infarction, Necrosis
5-10um, 10-15um, 25-30um, none
Mayer’s egg albumin, Starch paste, gelatin,
NOTA, AOTA
Canada Balsam, Water, Von Apathy,
Glycerin’s jelly
Schultz, Acrocarmine, Von Kossa, PAS
Flemming’s, Rossman’s, Heidenhain’s susa
Acetone
Overfixation
1 or 2 drops of alcohol, Mayer’s Albumin
Clinical Pathology
Brain, Lungs, Stomach? (GAUZE IS FOR
LUNGS, COTTON IS FOR
INTESTINE/STOMACH)
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