PLNT 4330 Tutorial 2 Altered Segregation 1) The shape of radishes may be long (LL), round (L’L’), or oval (LL’). Colour may be red (RR), white (R’R’), or purple (RR’). If a long white strain is crossed with a round red strain, what phenotypic proportions are expected in the F1 and F2? 2) What are the test cross generation’s genotypic and phenotypic ratios for the test crosses of dihybrids (i.e., F1 crossed to aabb genotype), which display the following types of epistasis in the F2? a. Single recessive epistasis (i.e. AaBb x aabb) b. Single dominant epistasis c. Duplicative recessive epistasis d. Duplicate dominant epistasis e. Recessive and dominant epistasis 3) A corn geneticist worked with two different strains of corn, one abnormal for chromosome 10, the other normal for chromosome 10. The gene C (coloured kernels), c (colourless kernels) was studied in a series of crosses. Cross I: F1: C (abnormal chr 10), c (abnormal chr 10) (coloured phenotype) Selfed F2: 18,295 coloured kernels : 6,095 colourless kernels Cross II: F1: C (normal 10), c (normal 10) (coloured phenotype) Selfed F2: 12,040 coloured kernels : 3,960 colourless kernels Cross III: F1: Female heterozygote C (abnormal 10), c (normal 10) x male testcross c (normal 10), c (normal 10) TC1: 27,799 coloured kernels 17,724 colourless kernels : Cross IV: F1: Female heterozygote c (abnormal 10), C (normal 10) x TC1: 18,647 coloured kernels male testcross c (normal 10), c (normal 10) : 26,833 colourless kernels Fully explain these results. Statistical analyses are not required. 4) A wheat geneticist worked with two different strains of wheat, one resistant to stem rust (Timstein), and one susceptible to stem rust (Chinese Spring). The gene Sr11 (stem rust resistant) and sr11 (stem rust susceptible) was studied in a series of crosses as follows: Cross I: Female (F1 of Timstein x Chinese Spring cross) F1: Sr11 sr11 x TC1: 17,875 stem rust resistant : Cross II (reciprocal cross of Cross I): Female (testcross) F1: sr11 sr11 x TC1: : 14,830 stem rust resistant Cross III: Chinese Spring P: sr11 sr11 male (testcross) sr11 sr11 17,857 stem rust susceptible male (F1 of Timstein x Chinese Spring cross) Sr11 sr11 0 stem rust susceptible Timstein Sr11 Sr11 F1: Sr11 sr11 Selfed (stem rust resistant) F2: 13,325 stem rust resistant : 0 stem rust susceptible Fully explain these results. Statistical analyses are not required.