EFFICACY OF DIFFERENT LEVELS OF ROOT HORMONE TO THE ROOT ENHANCEMENT OF BREADFRUIT (Artocarpus altilis) CUTTINGS THROUGH HYDROPONIC SYSTEM Submitted to: FELOMINO B. ANDIG, Ph.D. CARAGA STATE UNIVERSITY Ampayon, Butuan City In Partial Fulfilment of the Requirement for the Course Plant Propagation and Nursery Management (CS 201) Submitted by: GENEVIE FAITH S. SAJULAN FE L. DELGADO JIMMY JR. A. SUGSE MARIEL L. DELGADO ELMER C. MANATAD Master of Science in Crop Science (MSCS) INTODUCTION Nature and importance of the study Breadfruit (Artocarpus altilis) is a species of flowering tree in the mulberry family, Moraceae. The breadfruit tree provides a varied range of staple foods and has a high economic value (Soetjipto and Lubis, 1981). The fruit can be cooked and eaten in all stages of maturity. The denomination of the breadfruit certainly stems from the fact that its transformation could give bread products. The fruit would then can be used for bread making. Bokossa et al. (2016) showed that bread flour added to that of sorghum and soya is an important staple in the fight against child malnutrition. In addition to producing abundant, nutritious, tasty fruits, this multipurpose tree provides medicine, construction materials, and animal feed. Abimbola et al. (2010) found out that breadfruit could also be used to replace maize in animal feed, up to 22.2% in feeding rabbits without changes in digestion or feed quality of meat. All parts of the plant are also used medically especially the latex, leaf tip and inner barks. The latex is massage in to skin to treat broken bones and sprains. Diluted latex is taken internally to treat diarrhea and dysentery. According to Laura B. Roberts-Nkrumah’s manual “BREADNUT AND BREADFRUIT PROPAGATION”, most breadfruit cultivars grown for food, including the ‘Yellow’ and the ‘White’ are seedless, therefore, they have to be propagated using vegetative methods, which do not require seeds. Instead, vegetative methods of propagation use the vegetative organs of the plant such as shoots and roots. The use of root cuttings is the most suitable method that is currently available for commercial production of young breadfruit plants (Plate 4). Root cuttings can be used to produce young breadfruit plants because they can be induced to produce adventitious shoots. This method is the most suitable for commercial nurseries or for producers who plan to establish medium to large orchards or even smaller producers who plan to establish all their breadfruit plants in the field at once and have appropriate nursery facilities. The physical requirements for the nursery are greater but many more plants are produced by this method which reduces the risk of damaging the mother trees by frequent root removal. In propagating cuttings, hydroponic system is now introduced. Hydroponics, by definition, is a method of growing plants in a water based, nutrient rich solution. Hydroponics does not use soil, instead the root system is supported using an inert medium such as perlite, rockwool, clay pellets, peat moss, or vermiculite. The basic premise behind hydroponics is to allow the plants roots to come in direct contact with the nutrient solution, while also having access to oxygen, which is essential for proper growth. Breadfruit is valued in the community for it is beneficial to man for the usage on its different parts but only few propagates it. Since most propagation used soil media, the root can not consume directly the nutrients. Because of this, hydroponic system can be used where nutrients will be consumed directly by the plant. Objective of the Study This study will be conducted to find out the efficacy of the different level of root hormone to the root enhancement of breadfruit through hydroponics system. Specifically, this study will attempt to answer the following: 1. What is the average number of roots produced by breadfruit as affected by different level of root hormone? 2. What is the average length of the roots produced by breadfruit as affected by different level of root hormone? 3. What is the average number of leaves produced by breadfruit as affected by different level of root hormone? 4. What is the average survival rate of breadfruit root cuttings as affected by different level of root hormone? Significance of the study The result of this study will serve as a basis for both local and national development planners, in improving and developing the rooting techniques and practices of breadfruit through cuttings with the application of the different level of root hormone in a hydroponics system. There is no significant difference on the average number of roots of breadfruit root cuttings as affected by different level of root hormone. Scope and Limitation of the study This study will focus only on the survival rate of breadfruit root cuttings as affected by different level of root hormone, average number of roots produced by breadfruit as affected by different level of root hormone and average number of leaves of breadfruit as affected by different level of root hormone. CHAPTER III MATERIALS AND METHODS Time and Place of the Study The experiment will be conducted at Pananay-an, Placer , Surigao del Norte from the month of November to December 2018. Experimental Design The experiment will be laid out using Complete Randomized Design (CRD) with three (3) treatments and three (3) replications at three (3) sample plants per treatment. The treatments are as follows: CODE ROOT HORMONE T0 0 T1 100mL T2 200mL T3 300mL Field Layout T1 T4 T3 T2 T2 T1 T4 T3 T3 T2 T1 T4 T4 T3 T2 T1 Source of Root Cuttings Root cuttings of breadfruit will be collected from a fruit bearing breadfruit. Collection and Planting of Root Cuttings Root cuttings will be collected from a mother plant which are active, healthy and disease free. Root cuttings will be collected early in the morning to prevent from welting and dehydration and be planted right after collection of cutting. Sharp cutting tool (knife) will be used to prevent damage of cuttings. Care and Maintenance Newly planted root cuttings will be placed under a partial shady area to prevent from dehydration. A hydroponic system will be used. 11 Data to be gathered: 1. Days of Recovery. This will be counted from planting to shoot emergence. 2. Survival Rate (%). Survival rate of three (3) sample root cuttings per treatment will be computed upon termination of the study. It will be calculated by dividing the number of root cuttings that survived over the number of root cuttings used per treatment, that is: 𝑆𝑅 = 𝑁𝑜. 𝑜𝑓 𝑟𝑜𝑜𝑡 𝑐𝑢𝑡𝑡𝑖𝑛𝑔𝑠 𝑠𝑢𝑟𝑣𝑖𝑣𝑒𝑑 𝑋 100 𝑁𝑜. 𝑜𝑓 𝑟𝑜𝑜𝑡 𝑐𝑢𝑡𝑡𝑖𝑛𝑔𝑠 𝑢𝑠𝑒𝑑 3. Number of Leaves. Number of leaves of three (3) sample root cuttings per treatment will be counted upon termination of the study. 4. Height of roots (cm). Height of three (3) sample root cuttings per treatment will be counted upon termination of the study. Statistical Data Analysis All data gathered will be organized, tabulated and analyzed statistically using analysis of variance (ANOVA) in Complete Randomized Design (CRD). Duncan’s Multiple Range Test (DMRT) will be used to test any significant difference among treatments.