HO CHI MINH CITY UNIVERSITY OF TECHNOLOGY AND EDUCATION FACULTY OF CHEMICAL AND FOOD TECHNOLOGY SUBJECT: TEA, COFFEE AND CHOCOLATE PRODUCTION HOMEWORK 1-10 Instructor: Assoc. Prof. Le Hong Phu Student: VU THI THAO TRANG ID: 20116002 HO CHI MINH CITY, NOVEMBER 2021 Acknowledgments The thing I want to say is thank you, Mr. Phú and Ms. Nhi. Thank you for your willingness to teach and help me during this time. There are more and more things that I can learn and have to learn in the future. I hope that I can see you in the near future. Wish all best for you! Contents HOMEWORK 1 .......................................................................................................................... 4 HOMEWORK 2 .........................................................................................................................12 HOMEWORK 3 .........................................................................................................................17 HOMEWORK 4 .........................................................................................................................20 HOMEWORK 5 .........................................................................................................................23 HOMEWORK 6 .........................................................................................................................27 HOMEWORK 7 .........................................................................................................................32 HOMEWORK 8 .........................................................................................................................36 HOMEWORK 9 .........................................................................................................................40 HOMEWORK 10 .......................................................................................................................48 HOMEWORK 1 Question: What did you like most in the last lesson? Pick up a lot of stuff in my tiny box, but in the end, I never open the box again to view and look at it, this was one of the teacher's remarks that impressed me in class on September 10. A student who excels academically frequently commits the grave error of knowing too much and, as a result, is unable to be competent or professional at anything. I get a lot of new knowledge every day, and the amount of homework, exams, and projects I have to do make my head spin. Like other young people, I strive to study as much as I can as a member of the active Gen Z. I am now a third-year student as a result of that method of learning, but I still have gaps in my professional knowledge and am unable to connect them. After reading your remarks, I understand that I am putting in virtual efforts, keeping myself occupied but not fully assimilating the selflearned knowledge. But now I believe that instead of trying to design a beautiful scoreboard with little thought, I should concentrate on a subject that I love and am enthusiastic about. Perhaps among the majors that I am studying, I should find the subject that I am interested in and then start to learn more deeply. Then, when you graduate, you can bring with your in-depth knowledge and skills to meet the needs of employers. Everyone is only 24 hours a day, and can't keep focusing on useless things. From now on, I will learn to filter the items I see fit into my inventory and take time during the week to seriously look at each item I have put in the box before it gets old and damaged. Question: Find out my three favorite types of coffee in the last lesson Three favorite types of coffee are Weasel Bascd Coffee, Italian coffee and French coffee. 1. Weasel Coffee Weasel coffee is a unique coffee with an odd flavor that combines mustiness, syrup, and chocolate. Despite having a limited manufacturing volume, coffee is quite costly. a. History: Weasel coffee originates in Indonesia. When the Dutch first began farming on the islands of Java and Sumatra in the early 18th century, they came upon a unique species known as the Asian Palm Civet that could consume both plants and animals. They were then cultivating coffee plants in sizable farms to send back to their nation for usage and commerce. Of course, the owners of the coffee trees do not permit the local farmers who look after them to eat haphazardly. Then one day, they discovered that the ferrets also eat the coffee fruit, but the coffee beans are still in their shape. The farmers were allowed to collect the beans and they decided to clean them and roast them to make their coffee drinks. The taste of that coffee was delicious and quickly became known to the owners of Dutch plantations. Since then weasel coffee began to be known and widely developed and due to the specific nature of the production process, the price was extremely expensive and was dubbed the most expensive coffee in the world. b. Production process Stage 1: Coffee beans are digested in the weasel's stomach. After the weasel eats the coffee cherries, the coffee beans are affected by many enzyme-induced processes that transform and create a new flavor. Next, the coffee beans will follow the weasel droppings out and people will harvest them. Stage 2: Cleaning. This is the stage to remove the coffee beans from the ferret manure, ensuring that the beans are free of any stains or odors from the manure. Stage 3: Sun exposure Coffee beans, after removing weasel manure, will be spread evenly and exposed to the sun, helping to reduce the moisture of the beans to avoid microbial growth. Stage 4: Separating the husks The husk will not be digested, helping the coffee beans avoid infection when excreted. Therefore, this stage will remove the husk and classify and evaluate the coffee beans according to different standards before roasting. Stage 5: Roasting coffee beans This is the stage that determines the taste and quality of weasel coffee. c. Market price Weasel coffee in Trung Nguyen (updated September 14, 2022) is priced on their website as: 250 grams- 17 million VND. d. The reason why weasel coffee prices are high - In the world, weasel species are only distributed in certain areas and the amount of coffee that weasels can produce is also very little because weasels are carnivores, so coffee is not their regular food. - The habitat of ferrets is decreasing sharply, causing the number of mink to decrease sharply. Meanwhile, ferrets raised for production cannot produce good quality coffee like wild ferrets. 2. Italian Coffee In Italy, coffee is a need for daily living and is almost considered a religion. From Espresso to Caffé Shakerato, the menu offers a wide variety of Italian coffees. There are also two, three, or four various ways they may be prepared. I t must be said that Italian coffee is among the most flavorful and diverse types of coffee. a. Types of Italian Coffee 1. Caffè 2. Caffè Doppio (Double Espresso) 3. Cappuccino 4. Caffè Macchiato 5. Caffè Con Panna (Espresso With Whipped Cream) 6. Latte Macchiato 7. Caffè Latte 8. Caffè Ristretto 9. Caffè Shakerato 10. Caffè Ginseng 11. Caffè D’Orzo 12. Marocchino 13. Caffè Lungo (Long Espresso) 14. Caffè Americano 15. Caffè Corretto 16. Caffè Anisette (Le Marche) 17. Caffè D’un Parrinu (Sicily) 18. Bicerin (Piedmont) 19. Patavina (Veneto) 20. Moretta Di Fano (Le Marche) 21. Caffe ‘Allo Zabione (Bologna) b. History When the Paduan Prospero Alpino brought few bags from the East to Venice in 1570, the history of coffee in Italy officially began. The beverage was initially marketed at pharmacies, but due to its expensive price, it quickly gained popularity, especially among the more affluent classes. This, however, was insufficient justification for the failure of coffee, since the number of "coffee shops" quickly expanded, reaching 218 in Venice in 1763. At this point, coffee started to take on the role of beverage that most strongly evokes the sense of community and familial bonds that are among the fundamental principles of the Italian character. c. Information about some of the classics of Italian coffee - Espresso: Was invented by the Italians in 1884. In Italian, Espresso means "express", meaning coffee can be served to customers immediately. This coffee is brewed at very high pressure. The most standard is brewing with a machine invented by the Italians. On the surface of the coffee, there must be a layer of brown foam called crema, which is the standard to judge whether a cup of Espresso is good or not. - Cappuccino: Originates from Espresso but is somewhat more famous and popular in many countries around the world. A cup of Cappuccino consists of three equal parts: Espresso coffee mixed with double the amount of water (espresso lungo), hot milk, and foamed milk. The thick milk froth on a Cappuccino is created by stirring and frothing in a jug of fresh milk. The surface of the foam layer is sprinkled with a little cocoa powder or cinnamon powder to increase the aroma. - Latte Macchiato: Is a hot drink consisting of Espresso coffee and milk, basically like milk coffee but with more milk. The amount of caffeine in Latte Macchiato is exceptionally low. A standard cup of coffee must consist of three distinct layers, poured one after another without mixing. Milk is the first layer, then milk foam - is the highest layer. Finally, the espresso is poured into the glass through the milk foam. 3. Instant coffee Our favorite beverage, instant coffee, is a dehydrated form that is easily accessible and has the same fantastic flavor. Not to mention, making it is quick and simple. Other names for instant coffee include: Granulated coffee, Powdered coffee, and Soluble coffee. a. History David Strang of Invercargill, New Zealand, created and patented instant or soluble coffee in 1890. It was marketed under the trade name Strang's Coffee. b. Production process Step 1. Extraction. To produce instant coffee, the soluble and volatile contents of the beans, which provide the coffee aroma and flavor, have to be extracted. This is done using water. Step 2. Freeze drying. The coffee extract is frozen and cut into small fragments, which are then dried at a low temperature under vacuum conditions. Step 3. Spray drying. Coffee extract is sprayed into hot air, which quickly dries the droplets and turns them into fine powder or small pieces. Step 4. Decaffeination. In commercial processes, the decaffeination of instant coffee almost always happens before the critical roasting process which will determine the coffee's flavour and aroma processes. Step 5. Byproducts. The main byproduct of the instant coffee production process is spent coffee grounds. These grounds can be used as biomass, for example to produce heat used in the manufacturing process. c. Good or Bad Instant coffee may be made without a coffee machine and is quick and simple. It is also less expensive and has a far longer shelf life than normal coffee. As a result, it might come in handy when you're traveling or otherwise on the go. Compared to regular coffee, instant coffee has a little bit less caffeine and a little bit more acrylamide, but it also has a lot of the same antioxidants. In general, instant coffee is a wholesome, low-calorie beverage that has the same positive effects on health as other varieties of coffee. Question: Additives used in coffee. 1. Color improvers: These preparations have the INS designation 150a. 150c, 150d. 2. Odor enhancers: The smell of coffee is very specific and very attractive, so perfecting the aroma of coffee is of great interest to manufacturers. People can use ethyl alcohol (C2H5OH). Alcohol added to coffee near the end of the roasting process will react with some organic acids available in coffee to create esters with a pleasant aroma. 3. Substances that improve the taste of coffee: To create a strong taste for coffee, an appropriate amount of table salt (NaCl) is added. To create a pleasant "bitter and sweet" taste for coffee, some synthetic sweeteners can be used such as Acesulfame potassium (INS 950), Aspartame (INS 951), Sucralose (INS 955), and Sacarin (INS 954). 4. Coffee Thickness Enhancers: Coffee thickeners are produced using several thickening additives such as Sodium Carboxymethyl Cellulose (INS 466), Carrageenan (INS) 407), Aga Aga (INS 406), Gua Gum (INS 412) HOMEWORK 2 Question 1: What are methods to demucilage? The Amorphous Gel of mucilage around the bean consists of hemicelluloses, pestic substances and sugars and is soluble in water. It can therefore be removed by use of chemicals or mechanics a. Chemicals: Fermentation involves the beans being placed in plastic buckets or tanks and left until the mucilage has been broken down. Natural enzymes in the mucilage and feasts bacteria in the environment work together to break down the mucilage. The beans should be stirred occasionally and a few beans tested by washing them in water. The beans are ready when the mucilage can be washed off and the beans feel gritty. b. Mechanics: Based on mechanical rubbing, this process mainly makes the peel, flesh, and mucilage separate, and the coffee beans are cleaned. Because the pulp and mucilage of the fruit is separated from the bean by mechanical means, it is often left around the coffee bean and will have an adverse effect on the quality of the coffee. Question 2. Research pectin/cellulose, pectinase/cellulase. Clarify Question 3: What is the coffee fermentation for? The edogenous mucilage enzymes initiate the fermentation process, which includes the elimination of the mucilagegenous layer clinging to the parchment. Bacterial, fungus, and yeast fermentation then follow. Coffee tastes better after being fermented. Question 4. List out 3 interesting things that you have learnt in class. I was interested in 3 things in the previous lesson including: − Demucilage. − Thomas Edison's quote: I have not failed. I’ve just found 10,000 ways that won’t work. 14 − Dry method. a. Demucilage Demucilage means removal mucilage from coffee beans. The three main categories of elements that make up the mucilage that surrounds coffee beans are pectin, cellulose, and starch. It is this "mucis" that increases the time it takes to dry the beans and, in certain situations, to dry also encourages the growth of mold, lowering the quality of the product. Therefore, we constantly work to encourage fermentation, which is made possible by natural enzymes found in coffee cherries and environmental microbes. The production of several enzymes by microorganisms (yeast, bacteria) during fermentation plays a significant part in the reduction of mucus. b. Thomas Edison's quote: I have not failed. I’ve just found 10,000 ways that won’t work. Science is about doing experiments to find the solution. You can get some direction from a theory, but it may not always be confirmed by your findings. Facts, not emotions, are the foundation of science. Without extensive study and scientific advancement, we would not have the same level of information and comprehension of our world as we do now. Be confident and proud of your findings whether they support or refute your idea. Making a finding through experimenting, not getting it "perfect," is the most crucial aspect of science. Just be careful to explain how and why you believe your hypothesis was rejected in the conclusion. c. Dry method. To get rid of any bruised, immature, or otherwise faulty cherries, sorting is necessary first. The extra trash, such as soil and twigs, is often removed by hand 15 using a seive because the cherries are not cleaned in the same manner as they are in the wet process. However, cherries may be floated in a washing channel and the surplus can be taken out in this manner if one is provided. In the dry process, the fruit is still obscuring the beans. Only by frequently stirring the coffee can even drying be ensured. It may take up to four weeks, depending on the weather, for the coffee to achieve the desired moisture content of less than 12.5% because the beans are still covered by their protective coating of cherry. In order to make the process more efficient overall, larger farms and plantations may think about machine drying the coffee after it has slept in the sun for a few days. Large coffee silos hold the cherries until they are ready to be transferred to a mill once they have completely dried. The coffee will be hulled, sorted, and graded in the mill; this procedure will also get rid of the fruit 16 HOMEWORK 3 I liked 3 things that I learned in the last class. Includes "Every day we should ask ourselves 3 questions", a story about dancing goats and definition of Solid State Fermentation. The first is about asking yourself 3 questions. Up until now, my life has always gone by in a hurry. So I've never stopped for a minute in my life to think if I'm stopping learning and becoming a mindless learning machine. Through your words, I have been practicing for myself the habit of asking myself 3 questions early in the morning before going to school. Maybe it's a knowledge question just received yesterday or simple questions like are you happy today? Honestly, when I started asking the question I was a bit funny and had a hard time thinking about what I had to ask myself today. But 3 days later, it became easier and I also learned a few new things and felt more energized. I realized that by asking questions, sometimes it's just questions that sound stupid, but it makes my thinking better and trains the critical thinking skills that I've always been weak with. Your statement that "There are no stupid questions" is what made me realize that I have always been too self-conscious in voicing my opinions and thoughts. Because only asking questions is the essence of learning, making us absorb knowledge faster as well as remember it longer than usual. The second is a story about dancing goats. The story is attached to the history of coffee. A goat herder boy named Kaldi is on a mission to herd the goats for his father. 17 However, one afternoon, he did not see a trace of the return. After searching for a while, he finally heard a beep in the distance. Kaldi ran behind the bend of the narrow road, and immediately saw the goats. Under the thick canopy of the rainforest, light barely penetrates, the goats are running around, fighting with each other, legs jumping, and happily bleating. You think they've been enchanted. But as he watched the goats, he saw one after another munching on the smooth green leaves and red fruits of a tree he had never seen before. After a while the goats refused to come home with him, but none of them died. The next day, they ran right behind the cave and ate again like yesterday. So he tried them because he knew they wouldn't be poisonous. Legend has it that immediately Kaldi jumped up with the goats. He sings and dances around. Poetry and music flowed freely. Kaldi told his father about this magical plant. Thanks to Kaldi's discovery, the strange fruit trees spread very quickly to other regions, especially in the Middle East. The coffee cherries were shipped from Ethiopia to the Arab regions and grown in the territory of present-day Yemen. Then, when it spread to Turkey, they began to peel, roast and grind the coffee beans, creating the coffee we use today. So, the history of coffee began to take shape and expand as it is today. The final thing is Definition of Solid-State Fermentation. a. Definition of Solid-State Fermentation. Solid State Fermentation (SSF) is a fermentation method used by several industries like the pharmaceuticals, food, textile etc., to produce metabolites of microorganisms using solid support in place of the liquid medium. It is defined as the growth of microbes without free-flowing aqueous phase. 18 The primary SSF-related feature is the resistance of microorganisms (bacterial and fungal cells) to catabolic repression (inhibition of enzyme production) in the presence of plentiful substrates, such as glycerol, glucose, or other carbon sources. Because of such characteristics, this process has some advantages and disadvantages of its own. 19 HOMEWORK 4 Since I missed school last week due to illness and didn't attend the class, I borrowed the presentation of my friends to read and draw out three things that I liked the most out of the two presentations. The first is the pectin layer, why to remove it and how to remove it by emzym. The principal components of the 0.4 mm thick coating of mucus, also known as pulp or pulpa in Spanish, which covers the coffee bean include water, polysaccharides, sugar, proteins, lipids, minerals, and acids. The coffee bean must be separated from this framework during the fermentation phase of wet processing, despite the fact that it makes up 15% to 22% of the weight of the mature fruit. It is this “slime” that will prolong the time it takes to dry the coffee beans and, in some cases, also lead to the growth of mold, reducing the quality of the product. So, we need remove it from coffee. There are several enzymes responsible for this. Enzyme pectinase can Break down complex polysaccharides of plant tissues into simpler molecules like galacturonic acids. And hydrolyzing the α-1,4-glycoside linkage in pectin. Or enzyme cellulase can degradation of cell wall and hydrolyze the β-1,4-glycosidic linkages of cellulose. The second thing is how to adjust the amount of enzyme is just enough. If you add too little enzyme and the fermentation time is not long enough, it will not enough to hydrolyze cellulose and pectin. But giving too much enzyme and fermentation time too long will happen 20 hydrolyze both pectin, cellulose and soluble solid. We need to have actively selected spontaneous fermentation or added commercial yeast strains to the processing to guide the positive changes in taste. Based on experiments to determine the dose and duration of fermentation to be able to control this process effectively. The last one is caffeine. Caffeine (1,3,7-trimethylxanthine) is the main alkaloid component in coffee fruits and the source for bitter taste of coffee. Caffeine is widely found in tea, cocoa and coffee, and is one of the widely used psychotropic drugs. Although caffeine as pure chemical has a clearly bitter taste (it can be used as a ‘‘bitter’’ standard in basic sensory tests), it plays only a minor role in giving a bitter tinge to the coffee beverage. Caffeine biosynthesis takes place in the leaves and in the pericarp, the outer part of the fruit. In aged leaves the caffeine content is lower. In the pericarp tissues, light strongly stimulates the methylation step of caffeine synthesis. When the seed inside the fruit starts growing, caffeine is translocated through the membranes and accumulates in the endosperm. The caffeine content of the coffee beans depends on species and variety, from 0.6% in Laurina up to 4% in some extreme Robustas; averages are given in Table 3. The caffeine content in dry matter base is not affected by postharvest processing, neither by the roasting. Although the roasting process occurs well above sublimation temperature, during it only a small percentage of caffeine vanishes. Caffeine content of coffee can be reduced by decaffeination. The process starts with 21 a steam treatment of the green coffee to soften the tissues, followed by solvent extraction. Legal requirements on the caffeine content apply to decaffeinated coffee for the final product for consumption, that is, roast and soluble coffees. The European legislation sets a maximum residual caffeine content of 0.3% for soluble coffee; 0,1 % for roast coffee. 22 HOMEWORK 5 I liked these 3 the most in last week's lesson. First is caffeine. Caffeine (1,3,7-trimethylxanthine) is the main alkaloid component in coffee fruits and the source for bitter taste of coffee. Caffeine is widely found in tea, cocoa and coffee, and is one of the widely used psychotropic drugs. Although caffeine as pure chemical has a clearly bitter taste, it plays only a minor role in giving a bitter tinge to the coffee beverage. Caffeine concentrations decrease as leaves and fruits mature, but substantial quantities of caffeine remain in mature leaves and fruits, even in aged tissues. This is a very weak base forming unstable salts. It is relatively stable in dilute acids and alkalis but can form a series of complexes with other coffee components, for example chlorogenic acids or polynuclear aromatics. In fact, this property is also used in the selective extraction of polynuclear aromatics from other foods. The caffeine content of the coffee beans depends on species and variety, from 0.6% in Laurina up to 4% in some extreme Robustas; averages aregiven in Table 3. The caffeine content in dry matter base is not affected by postharvest processing, neither by the roasting. Although the roasting process occurs well above sublimation temperature, during it only a small percentage of caffeine vanishes. The reasons for this modest loss of caffeine are complex, but the two major contributing factors are probably an increase in the sublimation point of caffeine as a result of pressure build-up within the bean and a poor rate of diffusion of vapour through its outer layers. 23 Second is decaffeination. The legal requirements for caffeine content that apply to coffee are very strict. The European legislation sets a maximum residual caffeine content of 0.3% for soluble coffee; 0,1 % for roast coffee. The question is, what can we do to reduce the caffeine content? Caffeine content of coffee can be reduced by decaffeination. Since I don't have much time, I'll just introduce some techniques of decaffeination. The process starts with a steam treatment of the green coffee to soften the tissues, followed by solvent extraction. We have four main methods. 1. Di-Chloride methane (DCM) 2. Ethyl Acetate (E.A.) – also called sugar cane technique 3. Swiss water 4. Liquid CO2 Details of the 2 simplest methods: 1. Swiss Water is starts by adding green coffee to a vat of warm water. All of the water will twirl the beans and pass through a carbon filter, then back to the coffee and so on. The carbon filter only takes out the caffeine but leaves all other compounds in the liquid. After all the soluble compounds are in the water, the green beans are taken out. The remaining soup is the liquid Swiss water. The soaked green beans that need to be decaffeinated are immersed in the liquid and continue the same circulation as the previous coffee. The only difference is that the water has already taken compounds from the previous coffee. Soluble compounds only move from one cell to another when they find a place of lower 24 saturation within a liquid. When there is an equilibrium, soluble compounds do not move and stay in the cell. The water is saturated with green coffee so the liquid doesn’t extract the soluble compounds out of the green bean. Meanwhile, the caffeine does get extracted into the soup and taken out by the carbon filter. The whole process takes up to 8 hours until the caffeine level is below 0,8% of its weight. 2. The Carbon Dioxide (CO2) Method is the most recent method. Coffee beans that have been steeped in water are put in an extraction vessel made of stainless steel to undergo CO2 decaffeination. Caffeine is then extracted from the coffee by sealing the extractor and applying pressures of 1,000 pounds per square inch to liquid CO2. The larger-molecule taste components are left behind while the CO2 serves as a solvent to dissolve and pull the caffeine from the coffee beans. The caffeine-filled CO2 is then transported to the absorption chamber, another container. Here, the pressure is released, the caffeine is left behind, and the CO2 returns to its gaseous condition. Pumped back into a pressurized container for reuse, the caffeine-free CO2 gas. Because of its cost, this process is primarily used to decaffeinate large quantities of commercial-grade, less-exotic coffee found in grocery stores. Third is benefits of trigonelline. Trigonelline is an essential component for triggering a uniform endogenous antioxidant defense system. But it has received great attention because its thermal degradation products are important from both a sensory and nutritional point of view. 25 The overall scent of roasted coffee and other drinks is influenced by trigonelline derivatives. The breakdown product of trigonellin, niacin (nicotinic acid), one of the eight B vitamins, and it’s also called vitamin B3. The key role of niacin inyour body is to synthesize the coenzymes nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP), which are involved in over 400 biochemical reactions in your body — mainly related to obtaining energy from the food you eat. Niacin is water-soluble, so your body does not store it. This also means that your body can excrete excess amounts of the vitamin through urine if they are not needed. 26 HOMEWORK 6 Question 1: What are the enzymes involved in tea production? In tea leaves, polyphenol oxidase and peroxidase are the most significant enzymes. When the cell walls of the leaves are damaged and the polyphenols are exposed to oxygen, a process known as oxidation, they are to blame for the enzymatic browning of tea leaves. The browning of apples, potatoes, avocados, and bananas is caused by the same enzymes. Heat can be used to denature or inactivate polyphenol oxidase and peroxidase, preventing browning. The reason why completed green tea leaves stay green is because this is one of the earliest processes in the creation of greentea (and why cooked apples or potatoes remain white). Around 150 degrees Fahrenheit deactivates polyphenol oxidase and peroxidase, rendering them inactive. Polyphenol oxidase (PPO), an enzyme involved in fruit browning, is a tetramer that contains four atoms of copper per molecule. PPO may accept monophenols and/or o-diphenols as substrates. The enzyme works by catalyzing the o-hydroxylation of monophenol molecules in which the benzene ring contains a single hydroxyl substituent to o-diphenols (phenol molecules containing two hydroxyl substituents at the 1, 2 positions, with no carbon between). It can also further catalyse the oxidation of o-diphenols to produce o-quinones. PPO catalyses the rapid polymerization of o-quinones to produce black, brown or red pigments (polyphenols) that cause fruit browning. Peroxidase are enzymes that catalyze oxidation-reduction reaction by mechanism of free radical that transform several compounds into oxidized or polymerized products. Peroxidase can be produced by bacteria and fungi. 27 POD was observed to oxidize theaflavins in the presence of H(2)O(2), leading to the formation of thearubigin, another black tea pigment Question 2: Clarifying fermentation in tea technology One method of classifying teas is based on the degree of fermentation: 1. Non and light-fermented These teas have a good deal of their original flavor left over. These include all types of green tea. Most green teas, including Dragon Well, stop the fermentation process by pan-frying, while a few also do so by steaming. White teas experience a very mild fermentation process known as withering. These lightly fermented, nonfermented teas are occasionally given a jasmine scent by being perfumed with jasmine petals. 2. Semi-fermented Semi-fermented teas broadly refer to teas that have been permitted to experience 10% to 80% fermentation. Semifermented tea has a faint yellow brown tint and a gentle, delicate fragrance when brewed. Based on the degree of fermentation, these teas can be further divided into three groups: Light (10% - 20%): Jasmine Tea (Pouchong scented with Jasmine petals), Pouchong Tea. Medium (20% - 50%): Oolong, Tung-Ting Oolong Heavy (50% - 80%): Champagne Oolong. 3. Fully-fermented Black teas are fully fermented. Tea from Black tea leaves have a dark red hue 28 and a sweet aroma of malt sugar 4. Post-fermented Post-fermented teas are those that have been first allowed to ferment, had the process stopped, and then allowed to ferment once again. Question 3: List 3 interesting things you have learned The first thing is yin-yang in food. A fundamental idea in Chinese medicine is the concept of yin and yang, which is applied to all facets of life and health, including nutrition. The dynamic equilibrium of opposing forces is symbolized by the term yin (which tends to be more feminine) and yang (which tends to be more masculine). And it's crucial to obtain nutrients from diet without upsetting this balance. Yang foods are associated with fire and produce heat: Alcohol, Apricot, Beef, Caffeine, Cayenne pepper, Chicken. Yin foods are associated with water and are more cooling:Apples, Bananas, Beans, Bean sprouts, Bok choy, Broccoli. Neutral foods fall somewhere in the middle: Carrots, Dates, Duck, Peaches, Peas, Pork. In order to establish a balance of flavor (sweet, sour, spicy, bitter, and salty), texture, and cooking methods, the various ingredients work together. It's important to have a balance of starch (rice or noodles), meat, and vegetables. Congee or soup should be served alongside dry dishes like a fiery stir-fry. A Chinese chef will even take into account the meal's color harmony. For instance, vegetables with contrasting colors, like ell peppers or scallions, should be served with diced meat. The second thing is Black Tea. 29 As with all “true teas,” black tea is derived from the Camellia sinensis plant. Leaves of the plant are crushed, curled, rolled, or torn and then left to oxidize before they’re dried and sold. The fact that the leaves are fully oxidized accounts for black tea’s strong, dark flavor profile. The oxidation process also decreases black tea’s flavonoid content a bit, but this brew is still loaded with beneficial properties. Black tea is often sold in some kind of blend, which will determine its flavor profile. The season and place where a tea plant was grown will also impact its flavor. As a general rule, black tea is produced in China, India, Sri Lanka, or Nepal. As we mentioned above, black tea is the most common type of tea (at least in the US) and comes in many varieties. Benefits of Black Tea is help support the body’s protection against oxidative stress (which happens when damaging free radicals are left unchecked in the body). Process black tea. 1) Withering Here, the thick and still immalleable leaf is rid of approximately 30% of its humidity. There are two methods: a) The natural withering where the leaves are spread out on laths which are covered with jute, wire or nylon nets. The withering time takes, depeding on the weather and humidity content of the leaves, between 14-18 hours. b) The modern withering in large troughs of a length of 25-30 metres which are covered with wire grids and are ventilated with large ventilators. These can also be used to warm the leaves, should it be necessary, to reduce the withering time to 8-12 hours. 2) Rolling 30 3) Fermentation This oxidation and fermentation process already starts with the rolling. The correct fermentation is very important for the final quality of the tea. 4) Drying 5) Sieving/Sorting The third thing is withering process in tea. When a tea leaf is taken from the tea plant, it starts to wither naturally, which is what we refer to as wilting. However, the tea producer has control over this process once the tea leaves arrive at the processing plant. By lowering the moisture level of the leaves, a controlled wither is used to get them ready for processing. In order to control the withering process, it is important to continuously monitor the air's temperature, humidity, and flow. Tea leaves can be let to gently wither outside on bamboo mats or tarps, or indoors in troughs with forced air. If necessary, the process might be sped up by heating the air. The moisture content of the leaf decreases during withering, making it flaccid and malleable. The leaf is now ready for shape and rolling, among other processing steps. On a chemical level, the leaf's chlorophyll starts to deteriorate, caffeine levels gradually rise, flavor and scent volatiles form in the leaves, and grassy odors fade. The leaves are deprived of their energy source, so they start to convert their stored carbohydrates into energy. The breakdown of the cell walls brought on by moisture loss also starts the oxidizing process known as polyphenol oxidase and peroxidase activity. The development of new flavor and aroma chemicals in the leaves increases with the duration of the wither. But when the leaves are withered over a long time, polyphenol and peroxidase activity will stop due to dehydration 31 HOMEWORK 7 Question 1: Cocoa fermentation, anaerobic and aerobic Cacao fermentation is a two-step – anaerobic and aerobic fermentation. In anaerobic fermentation, the cacao is protected from air to allow microorganisms like yeast to grow. Anaerobic conditions are simply environments that lack oxygen. In cacao, the pulp surrounding snugly packed beans creates a juicy barrier that blocks air from entering the system. The pulp is composed of water, high levels of sugars (sucrose, glucose, fructose), and various acids. These sugars and the high acidity in the pulp create ideal conditions for microorganisms. The main players during this phase are yeasts, lactic-acid-producing bacteria, and pulp enzymes. Using anaerobic respiration, yeasts quickly consume simple sugars and produce carbon dioxide, ethanol, and low amounts of energy. Lactic acid producing bacteria convert citric acid, glucose, and other carbohydrates in the pulp into lactic acid. Microbes are working around the clock to produce ethanol and lactic acid. You can see tiny bubbles of carbon dioxide gurgle up to the surface of the cacao in this stage. Enzymes also help to break down the pulp, transforming it into a liquid that runs off and is known as sweatings. As the mass is broken down, there is more space for air to enter the process. Citric acid is also broken down and runs off with the sweatings, helping to increase the overall pH of the fermentation. The combination of rising pH and increased airflow marks the beginning of the aerobic phase of fermentation. Anaerobic fermentation also creates heat, which destroys the germ of the seed, which is particularly bitter. In the second state, air is introduced to promote aerobic fermentation. Aerobic 32 conditions are environments that contain oxygen. In cacao processing, producers mix and move the beans, a process known as “turning”, to incorporate oxygen. The influence of this oxygen varies based on fermentation style, vessel, insulation, and the size of the ferment. But turning the beans will always make the fermentation process more uniform across the batch. The aerobic stage is bursting with heat. During this step, acetic-acidproducing bacteria dominates and oxidizes ethanol and acids (citric, malic, lactic) to produce acetic acid. This acetic acid is further broken down into carbon dioxide and water by the oxygen. Breaking down ethanol generates energy, which is expressed as heat. These exothermic reactions increase the overall temperature of the cacao. As the beans are turned, heat escapes and the overall temperature drops, but it then builds again through the introduction of more oxygen. The combination of intense heat and the diffusion of ethanol and acetic acid into the cacao beans breaks down the cell walls. They will no longer germinate and the damaged internal structure becomes a venue for chemical activities that develop the flavor precursors associated with chocolate. Question 2: Fermentation in HEAP A common technique used by smallholder farmers in Ghana and many other African nations that produce cocoa is heap fermentation. A lesser degree of it is also practiced in Brazil's Amazon region. According to the characteristics of Ghanaian cocoa's final quality, fermenting in heaps might result in high-grade goods. From 25 to 1,000 kg of cocoa beans are piled up in the field on plantain leaves and covered with the same substance. To 33 guarantee equal fermentation and reduce the risk of mold formation, the beans are periodically combined (turned). By creating another heap, this is frequently done every day or every other day. It takes a lot of work to mix, and little heaps might not even be turned. Fermentation takes 4 to 7 days to complete. Question 3: List out 3 interesting things that you have learnt in class. First is what is cocoa. Cacao is a "pure" form of chocolate, meaning it's raw and less processed than cocoa powder and the chocolate bars we're used to seeing. Cocoa is believed to be the highest source of antioxidants and magnesium of all foods. Cocoa has been used for a long time for health purposes and is also a high value commercial commodity. Cocoa tree, also known as Theobroma Cacao. The cocoa pods are harvested and the pods cracked to get the cocoa beans inside. From there, cocoa beans are processed in different ways. Cocoa butter is the fattest part of the cocoa fruit, located inside the skin and covering each cocoa bean. It has a white color, a buttery texture and a taste similar to white chocolate. Cocoa butter is separated from the beans during the manufacturing process. The remainder of the cocoa pod is used to produce raw cocoa powder. Cocoa chips are cocoa beans that are chopped into edible pieces, similar to chocolate chips but without added sugar or fat. Cocoa chips include fiber, fat, and many of the same natural nutrients as cocoa beans. 34 Second is a person's appearance are benevolent, from the good and evil heart of each person I will say this part in Vietnamese. Quan điểm phật giáo cho rằng tâm sinh tướng chính là biểu hiện của việc tâm hồn có bao dung hay hèn kém sẽ thể hiện qua từng ngũ quan trên cơ thể gương mặt cũng như hình hài lẫn dáng đi đứng nằm ngồi. Người hiền từ, tâm tốt và luôn biết nghĩ cho người khác, sống một cuộc đời không vướng bận và suy nghĩ đố kị sẽ luôn có gương mặt phúc hậu, gây hảo cảm cho người đối diện dù chỉ là một cái chạm mắt. Họ thu hút những người tốt xung quanh đến với họ, năng lượng tích cực khiến mắt họ sáng, gương mặt luôn rạng rỡ và khóe môi mềm mại để nợ nụ cười xinh đẹp. Trong khi đó, những người có tâm toan tính, lúc nào cũng có vẻ chột dạ và không đáng tin tưởng. Họ khiến người khác dè chừng và cảm thấy nên tránh xa, không khí quanh họ u ám. Và tất nhiên, cuộc sống của họ sẽ gặp những khó khăn nhất định, dù họ có được lợi ngay trước mắt thì ngay sau đó cũng tiêu tán nhanh chóng. Final is the fermentation in cocoa. It was mentioned above, so I would like to shorten it to a few issues. Cacao fermentation is a two-step – anaerobic and aerobic fermentation. Understand the difference between these two methods to determine for your product as well as take advantage of the advantages and disadvantages of each method to achieve the best effect. 35 HOMEWORK 8 Question 1: List out 3 interesting things that you have learnt in class. First is free and total fatty acid in coffee For the most part, the fatty acids are to be found in the combined state. Most are esterified with glycerol in the triacylglycerols. Approximate 20 % are esterified with diterpenes. There are small proportion is to be found in the sterol esters. In coffees roasted at different temperatures, there were scarcely any changes in the amount and distribution of the individual fatty acids. Only the linoleic acid content decreased slightly as the roasting temperature increased. Using a roasting series of a Madagascar coffee, the differences between the FFA contents determined directly by chromotography and those determined indirectly via the acid value could be demonstrated (Fig. 1.14). While the difference is only about 360 mg for green coffee, it becomes steadily greater as the roasting temperature 36 increases; at the last roasting stage the difference is 1300 mg. This can be explained by the fact that more and more acid compounds are released as the temperature rises. These are primarily phenolic degradation products of the chlorogenic acids that considerably distort the results for the free fatty acids Sencond, is diterpenes in the lipid fraction of robusta and arabica coffees. The main diterpenes in coffee are pentacyclic diterpene alcohols based on the kauran skeleton. The research worked for several years to identify the structure of two of the coffee diterpenes, namely kahweol and cafestol. Both are sensitive against acids, heat and light, and kahweol in particular is unstable in the purified form. Another important group of diterpene derivatives found in coffee is the atractylosides, which are mainly present as glycosides. Arabica coffees contain cafestol and kahweol, robusta coffees cafestol, small amounts of kahweol and, additionally, 16-O-methylcafestol (16-OMC), which was found only in robusta coffee beans. 37 Finally, is about coffee wax. The surface of green coffee beans is covered by a thin waxy layer. Coffee wax is generally defined as the material obtained by extracting it from coffee beans using chlorinated organic solvents. The amount of the surface wax is about 0.2 - 0.3 % of the total bean weight. 38 The amount of the surface wax is about 0.2 - 0.3 % of the total bean weight. The main constituents of the petroleum ether insoluble part of the coffee wax are called carboxylic acid-5-hydroxytryptamides. Even though the C-5HTs are the main constituents of the coffee wax, it is unlikely that they are solely responsible for the undesirable effects of untreated coffee. The antioxidant effects of the C-5HT have led to a great interest in coffee wax as a natural antioxidizing agent to be used in food 39 HOMEWORK 9 Question 1: List out 3 interesting things that you have learnt in class. First is History of Cocoa. Cocoa (Theobroma cacao L.) is a local species of tropical humid forests at the decrease japanese equatorial slopes of the Andes in South America. Allen (1987) pronounced the centre of genetic variety of T. cacao to be the Amazon The phrase cacao is derived from the Olmec and the following Mayan languages (Kakaw) and the chocolate-associated time period cacahuatl is Nahuatl (Aztec language) derived from Olmec/Mayan etymology. Based on archaeological information, Purdy and Schmidt pronounced that the Mayans cultivated cocoa 2,000–4,000 years earlier than Spanish contact. It is recorded that cocoa changed into domesticated and ate up for the primary time via way of means of the Maya and Aztecs. The Maya, Olmec, Toltec and Aztecs used the beans of cocoa as each foreign money and because the base for a bitter drink. The call cocoa is a corruption of the phrase cacao, which originated from the Amazons in South America. Its cultivation and price unfold in historic instances at some stage in central and japanese Amazonia and northwards to Central America (Afoakwa 2010). Cocoa changed into first cultivated via way of means of the Aztecs in Mexico, South America, and unfold at some stage in the Caribbean islands. 40 The principal varieties of the cocoa tree Theobroma cacao are: 1. Forastero from the Amazonas region, and grown mainly in West Africa as bulk cocoa 2. Criollo, rarely grown because of disease susceptibility 3. Trinitario, a hybrid of Forastero and Criollo 4. Nacional with fine flavour, grown in Ecuador Second is Process of Chocolate Once the cocoa beans have reached the machinery of chocolate factories, they are ready to be refined into chocolate. Generally, manufacturing processes differ slightly due to the different species of cocoa trees, but most factories use similar machines to break down the cocoa beans into cocoa butter and chocolate (International Cocoa Organization, 1998). Firstly, fermented and dried cocoa beans will be refined to a roasted nib by winnowing and roasting. Then, they will be heated and will melt into chocolate liquor. Lastly, manufacturers blend chocolate liquor with sugar and milk to add flavour. After the blending process, the liquid chocolate will be stored or delivered to the molding factory in tanks and will be poured into moulds for sale. Finally, wrapping and packaging machines will pack the chocolates and then they will be ready to transport. A diagram showing the manufacturing process: 41 Some important step: Step #1: Roasting and Winnowing the Cocoa The first thing that chocolate manufacturers do with cocoa beans is roast them. This develops the colour and flavour of the beans into what our modern palates expect from fine chocolate. The outer shell of the beans is removed, and the inner cocoa bean meat is broken into small pieces called "cocoa nibs." The roasting process makes the shells of the cocoa brittle, and cocoa nibs pass through a series of sieves, which strain and sort the nibs according to size in a process called "winnowing". 42 Step #2: Grinding the Cocoa Nibs Grinding is the process by which cocoa nibs are ground into " cocoa liquor", which is also known as unsweetened chocolate or cocoa mass. The grinding process generates heat and the dry granular consistency of the cocoa nib is then turned into a liquid as the high amount of fat contained in the nib melts. The cocoa liquor is mixed with cocoa butter and sugar. In the case of milk chocolate, fresh, sweetened condensed or roller-dry low-heat powdered whole milk is added, depending on the individual manufacturer's formula and manufacturing methods. Step #3: Blending Cocoa liquor and molding Chocolate After the mixing process, the blend is further refined to bring the particle size of the added milk and sugar down to the desired fineness. The Cocoa powder or 'mass' is blended back with the butter and liquor in varying quantities to make different types of chocolate or couverture Step #4: Tempering. Melting the chocolate while controlling how its temperature rises and falls This technique lets you use the chocolate the way you want to and get the perfect result for all your creations Cocoa butter has fatty acids that undergo polymorphous crystallization, meaning they can form six different types of crystal structures at any one time However only form bV—the second most stable crystal form—is desired in the 43 final product Tempering will control this propensity in order to obtain chocolate candy of the highest standard: lustrous, smooth, hard, crisp, and rich Finally, is Spoilage Problems with Chocolate. Chocolate Blooming is the term we use when we see that the chocolate has dusting or streaking across the surface. There are two types of blooms – fat bloom and sugar bloom. Fat bloom can be identified by greyish or whitish streaks on the surface of the chocolate that typically feels slick. This blooming happens when the chocolate has 44 been exposed to warm temperatures. Once the chocolate is warm, the cocoa butter in the chocolate softens and separates from other ingredients in the chocolate. Once it rises to the surface and re-solidifies, it creates the bloom. Fat bloom is the most common type of chocolate blooming. It is caused by: 1. Bad tempering of the chocolate in that stage of the process. 2. Incorrect cooling methods, including covering cold centers. 3. The presence of soft fat in the centers of chocolate-covered units. 4. Warm storage conditions. 5. The addition to chocolate of fats incompatible with cocoa butter. 6. Abrasion and finger marking, particularly under warm conditions. Sugar bloom can be characterized as a dry, hard white surface film on chocolate. Sugar bloom is caused by moisture in the chocolate coating. The sugar absorbs the moisture, dissolves, and evaporates. The sugar then forms larger crystals on the surface of the chocolate, which causes this dusty layer. 45 It is caused by: 1. Storage of chocolates in damp conditions or against damp walls. 2. Deposit of "dew" during manufacture from damp cooler air or allowing chocolates to enter a packing room at a temperature below the dew point of that room. 3. Use of hygroscopic ingredients (e.g., low-grade or brown sugars). 4. Removal of chocolate from cold storage without adequate wrapping protection. 5. Use of damp packing materials. 6. High-temperature storage conditions of chocolate covered confectionery where centers have a high equilibrium relative humidity (e.g., fondants) and the water vapor given off is trapped in impervious wrappings. Can Chocolate Blooming Be Avoided? Generally, yes. Fat bloom results from inadequate tempering or temperature abuse of well-tempered chocolate, therefore you can prevent fat bloom by storing your chocolate at a constant, cool temperature and avoiding warm temperatures. Sugar 46 bloom occurs when the sugar crystals are affected by moisture. This happens when the chocolate is stored in damp conditions (ex: humidity in the air or condensation from refrigeration), which causes the sugar to dissolve and come to the surface. You can prevent sugar bloom by preventing temperature shocks. Can You Still Use Chocolate That’s Been Bloomed? While fat bloom and sugar bloom have a negative effect on appearance, the product remains perfectly safe to eat and can be remelted to remove discoloration. 47 HOMEWORK 10 Question 1: List out 3 interesting things that you have learnt in class. First, is Benefits of tea. People all over the world have been drinking tea for thousands of centuries, and for good reason. Tea is an aromatic beverage prepared by pouring hot or boiling water over cured or fresh leaves of Camellia sinensis, an evergreen shrub native to East Asia which probably originated in the borderlands of southwestern China and northern Myanmar. Tea is also rarely made from the leaves of Camellia taliensis. After plain water, tea is the most widely consumed drink in the world. There are many different types of tea; some have a cooling, slightly bitter, and astringent flavour, while others have vastly different profiles that include sweet, nutty, floral, or grassy notes. And this has different benefits. White Tea Benefits White tea is made from the Camellia sinensis plant, which is indigenous to China and India and is known for having a delicate flavor. The least processed tea kind is this one. Due to its strong antioxidant content, research suggests it may be the best tea for preventing many types of cancer. Since white tea has a high concentration of fluoride, catechins, and tannins that strengthen teeth, fight plaque, and increase their resistance to acid and sugar, it may also be beneficial for your oral health. This variety also has the lowest caffeine content, making it a wise choice for tea drinkers who want to avoid or consume little to no caffeine. Herbal Tea Benefits Herbal teas, sometimes called tisanes, are very similar to white teas, but they contain a blend of herbs, spices, fruits or other plants in addition to tea leaves. Herbal teas don’t contain caffeine, which is why they’re known for their calming properties. There are numerous types of herbal teas, all with their unique benefits. Some of the most popular herbal teas include: Chamomile tea – Helps to reduce menstrual pain and muscle spasms, improves sleep and relaxation, and reduces stress Rooibos – Improves blood pressure and circulation, boosts good cholesterol while lowering bad cholesterol, keeps hair strong and skin healthy, and provides relief from allergies Peppermint – Contains menthol, which can soothe an upset stomach and serve as a cure for constipation, irritable bowel syndrome and motion sickness. This tea variety also offers pain relief from tension headaches and migraines. 48 Ginger – Helps to fight against morning sickness, can be used to treat chronic indigestion and helps to relieve joint pain caused by osteoarthritis Hibiscus – Lowers blood pressure and fat levels, improves overall liver health, can starve off cravings for unhealthy sweets, and may prevent the formation of kidney stones Green Tea Benefits Green tea comes from China, where the leaves are heated up and processed through roasting or pan-firing, and Japan, where the leaves are more frequently steamed. Green tea has a particularly high concentration of flavonoids, which can improve heart health by lowering harmful cholesterol and blood clotting. According to studies, this kind of tea can also decrease triglycerides, cholesterol, and blood pressure. Green tea may have an effect on cancers of the liver, breast, prostate, and colorectal, according to additional research. This particular tea variety has also demonstrated anti-inflammatory properties, which support healthy, clear skin. Matcha, a type of green tea, has gained popularity recently, is a very fine, highquality green tea powder made from the entire leaves of tea bushes grown in the shade. Since it is the only form of tea in which the leaves are ingested, matcha contains even more antioxidants that regular green tea. In fact, some have suggested that one cup of matcha is the equivalent to 10 cups of regular green tea. Black Tea Benefits The same plant that is used to produce green tea, Camellia sinensis, also produces black tea. Black tea, on the other hand, has a darker color and fuller flavor since the leaves are dried and fermented. Black tea contains caffeine, unlike many other types, therefore it's vital to limit your consumption. If you do make yourself a cup of black tea, you gain the advantages of flavonoids that reduce inflammation and promote a strong immune system. However, in order to take advantage of black tea's health benefits, you don't just have to drink it. To relieve pain and lessen swelling, it can be steamed, cooled, and then applied to small cuts, scrapes, and bruises. Oolong Tea Benefits Oolong tea is a traditional Chinese tea variety that’s made from the same plant used to make green and black teas. The difference is how the tea is processed: Green tea is not allowed to oxidize much, but black tea is allowed to oxidize until it turns black. Oolong tea is somewhere in between the two, so it is partially oxidized. This 49 partial oxidation is responsible for oolong tea's color and characteristic taste. Oolong tea is notable for containing l-theanine, an amino acid that reduces anxiety and increases alertness and attention. Scientists have found that l-theanine can help prevent cognitive diseases such as Parkinson’s and Alzheimer’s diseases. Oolong tea is also high in polyphenols, which are linked to lowering inflammation, preventing the growth of cancers and decreasing type 2 diabetes risk. Second, is why does traditional tea become popular? Surely everyone has known about tea, but to say when tea will be widely known, not everyone knows. According to some research documents, tea appeared more than 5000 years ago in China, but at that time, tea was like a food, often used to prepare with some other foods or eat like vegetables. After tea began to flourish in China, in the 9th century under the Tang Dynasty, a Japanese monk brought the first tea tree to Japan, also from here the emergence of the culture of "Tea" Tao" is famous all over the world. Around the seventeenth century, when the exchange of goods between Europe and China flourished, tea was also followed by shipments and developed to this day. In 2017, Mansci Professional Services, an organization specializing in market research, researched and found that tea is the second most popular beverage in the world after bottled water. Besides, up to 80% of households in the US use tea at home. Finally, is turning waste tea to wealth. Extraction of useful compounds: Black Tea Polyphenols (Catechins): excellent health promoting activities, reduce blood pressure and prevent the growth of cancer cells. Tea Polysaccharides (leaves & buds): used as natural antioxidants & potential candidates for application in the feed industry. Formation of different materials: A promising source of several valueadded materials: activated carbon, nanoparticles, biochar and other cellulosic derivatives. The application in textile industry wastewater, environmental, biomedical and pharmaceutical sector and also in polymer composite industry 50 51