Identify and explain what property is used to separate the fragments from
each other. /2
Cl t \ e le.d \"> ~ h,o ,~_ns. -
DNA
I
c. 4,
'2.
0 °'
-h-.U j IY,-€
O£.
{11-- i
In DNA
a.
b.
c.
Si
.
){
.,.,
\ lIP.
J
14 _ '.'
~.'
"J '
~
l.
._
_______~____'_'_____
- ?' '_i_r________
er(.. :. J....
'0
~
1
d(, •
(
,
('<
•
UI"­
~ .f'
• ."""1'"
\
r-e p '-, .. -c,
...""q=-\.\"'·c-\-'I ........, ~ j
\reOl \
v-J(). y .
'c
.. !
(~
.--1
C' r'
t
~ ,I
,
- P
-'
f", " l\
..J
'\I~
r •
I
r"f
f'o,rvi
"',
r n'"
.
Ir
1.
{.,('.. '1
-\~rr
l
".:1.':-
(e l
,. ('. I/ ! I
ye
replication, what would happen if.. .. be specific with your answers; no generalizations. /3
Single-stranded binding proteins were malfunctioning?
Primase was inactivated?
, ~<: •
..... ~ ".,
DNA polymerase I was malfunctioning?
{'
(01 ~:'l't
r
I'
;l ! !. f'\
r
• 11
,
1,(
rc'':(1)
,.. t'\
... .
II
r • ..,
I
'-'·:1"'I-'~. ~-:r~d,J ; ~II'
ra-);
-{YOjf'.-JV\+-;.
01:0\. 7'''' \'- 1
•\'
'N.J..\be (,"/'.' -t,..~ (~ ... l (" " • ( C\ 11.( ',.1
. \1 'c~
, ~, '" \ I,/. I I ~ • h y ! i .j~J i ')
Drawa series of diagrams to show how lagging strand replication takes placejin a cell. Label all the
relevant structures in your diagrams . /6
PJJn
.JI ire
CA.
.. . '"
: .
; \"v
(· <!(.o .... r:('
'1(!i
o·
3.
r ....
\""01' J , t~
D '''I~
1/'.1
",.)!e,
..!..J"''' t ./~
-~.,
j"
n N~.
vl·\+t..
p.ro.t! r
i"l)~,~"
L: . r~-~. r /./
Which two fragments may be difficult to isolate on a gel? Why? /1
\~
2.
(
,
r pl,vA,.,,,
---
.;~
pr' ''''~J
f: ~,-~
" " frr· ·c
... 4,..£ ....
l' ,
-'v
R""
1" ""', .
'f==G----e---:.'
,RN A
~
')NP- , \~~
~""
<:, ,,,
.\..l'e
'0', (I ~
+0
1'''''0
s.L(
i
1 \"01(,.\=
r
Io.~~;r .~ ~Tv.""
-+0
r
oJ
sf r ' I'~
ONt\ pol'1 ~~ e
'
b]Vp
s.tro...J>
_~.
J
I 0 ~ ~\ ko
~~ DNA
--="'--'-I
r
'l
@ 1~ e
l N i'­
0 \<'0. '2':;\ Ie ~
l \~",
r
t r"~ W'(~: r
'"')
.1-0
c;red; ~'"
-:;'
-t-c...J~l\ +s,
t
~ '1
i >'\
."
--------------- y
G5
'r..\.A6Ia l e ',r
I tiihoA I' V\
l
~.\.eJ
",o.~ { ~ '''' -t~e
+e
:>"
h,d.-.X'1i
Ii.
+~
o. {I.d.
? rO""~\' ¥-\\! ,,,,k r-e + ' C\ "~t. < : rho" d· 11 ~
%r-td · T~. p',"*"o f.t...
I\lsr "" r. tJ A :f.. be
T\
of mh ~<;~p~l~4e !>;!~
r '
h · J~!"'4-
"
t,r· , L' r
Q.
.Y\ RN
o·
~.\ e,-
C'
Nlc,("".'"
t ,... f,. rJ"~
r\
.~.,.
I)
(Qw'f,lew,q.,Ja "1 f-Wc.
DN 1'\ ,+", ,,, l.
3. I_S'
~,~
' ;
.
'II
-'
3I
e:
.f',.,_
~(\...t?~
~).-T"~ f'I ,I
c. ' :A.,(:
f'
5. Draw the double helix structure of DNA below (2 marks): "
....
~
c·" •
- I
..J
f:>
ro I
A .:­
J~S
', 1
C.'1 ~ rl " J )"" .
51 -A C G-3 '
3' - T G C - 5'
'1 _
V
label:
A. All parts of the nucleotides (3 marks) [Stc...L¥- fl~Y\
B. Identify the ba ses as either purines or pyrimid ines (3 marks) ~c
C. Label all bond s between ba se pai rs (2 marks) l)\v.e. ~
1
D. Labe! t he 5' and 3 ends of ea ch strand (2 marks) ~
fD
l
<?l'1
CD
\
OIl.
;). H'f6..,;)~eV\
\.-\0
\.c \,\ .lf
~
.
~
h~dV\? )<LJ(
eoll cl
of
Jf
I~
Pt Vl L i \
Inquiry (12 marks)
1. Below are 2 DNA sequences of an individual. Over the years, this individual has been exposed to toxic
chemicals resulting in a mutation of their genes. Sequence 1 is the individuals original DNA and
sequence 2 is the mutated strand . Knowing that the introns are from letters 10 through 15, write the
mRNA sequence, the mRNA with n:odifications, and the amino acid sequence for both sequences.
Then answer the questions on the next page. /6
,
r
mRNA:
I
_
_
Amino Acids:
~i'-\
•
~-
fV'o L ~ ; e.J ' "",.e.+ - hi:' - \
mRNA:
CAC
- hi 5
- \
C \J
(..,(1 A UIJ C 'VA,,",
A
VP-0,
PtUL C...uU
P.U (
(V v
- po l'1 PT .p,,' -J/
(­
e\!' -<j\ -f\"'~ - st o [> .~
-5l", -pl.e (~ .j.-Q~
ev.
AAT!:M.1A(G.GACACG4~~~_GA
DNA sequence 2: 3' -
./
1""0 - <j \,
IV,e.,+- -
uVC
~u
with mRNA S"' - c f' - UlJ A AV\J tu(.
modifications:
,
c., Af'
l)vA AOV A v e". u v GqJC"'l cP- c.. cvr-
,, ' - l"IJ I'\ p..v lJ AV
CTAG GAA - 5'
~ VL'l cr..c. CU I'" VAI~
n C(U
\ )\JC
vAGl
P.V C-
CVV-3 '
~
"";). -J
withmRNA
I
I
modifications: S - v.~ci.o
Amino Acids:
~7
•" RN f\ ~ "'" e+. ~J! f'\ e J. . i""'f.l-
p YV
!l\)!'J..·.rl-:..(. ,~Up.,.vAf' Vuc.. \jp.C".,Aue..
WI:>. P. ()
{(}"p -
-
V
"
Ij \ ~ -
~. $
r
I ~ v\
-
- (
s-" 0 (»
.
a,
- h. t, - \ e\A - (~f ) .
What tvpe of mutation is contained in sequence 2?
b.
Describe how this mutation would affect the organism. /1
IV
"c,e" !,e
P"tV\+
.\-~
/VI 1I.1
G"'~
15'
etf I ) '"
f ro kl l\
TI-,"1
of ··H'l.
'..;
'\
V.l" L.~
oV
J
,,- ,\ 1
f'-'>
"J
flY".
(uU-r)
... ,
/
_,,;
_'..
".J"'rP ~~~r
ev+-
~~
")~'" IA
rlu+_,,~ I
t,
r•
.,,+
.
{
~
c-
.,-lw".P_
I'
\I~
~
- -- -- -- --
bit:
/7 (r.')r ;(I' jp )
/1
- ! .. ~
'11 , ,,
I
-j'
c.
Using any part of the DNA sequence at least 6 bases long, show and describe a silent mutation.
Accurately show and describe the error by comparing the original code with the mutated code
for DNA, mRNA, and the amino acid sequence. Also, identify the mechani sm used to cau se that
type of mutation (insertion/deletion/substitution). /4
3' -1=A C. - Af1 T - GL\ ~ A11 -5 '
;:J ~i\I!."'+
"
e. '{c..... pl.~
Q;" ........' \\ "
D
yY\e.+ - levi -
' :)r-
0.."", ',. ~...--
c..
s-,\"(·\'.-\v,.j.' "".
r "'~+
eejlAl ", ·(
"",,,,i,,,~;,,,s
?+
f'xO" ... f · o,
f"" JVI <..(. .!
i>""~<--'c;J'
1>-'3 - [ d QP ) .
---------------~
C·...
r
'.. \ l }
~I..IJ
1
it
e.
-1., r
' '"'
()
DtJ A ~ -3
I
-
i P. C. - A P,l ­
~
c.
..... < ,
- ~ /
A ,- A Ti
/ _ P.LJ~ -vVA-C~ -IJP.iJr-]'
) L.,o,'i '
a£+et,.,. +4. 0..'''''''<) ",.J,
'e
'
,
L
\
I
~
(> h ., D " " ' . , .
lv t
O\ ~ ;" o
v'.
' ."" e, - e ", - ""!j
[VI",-h;\t,.1V\ ~
,
~ .
,\1\; ,
r r
ApplicatIOn (10marks)
C.; I) ., ; 11'.-'_
' ._
.,""
,' .. \
J '('.
1. Explain what would happen if a drug became attached to a specific repressor protein and prevented
the protein from binding to an operator gene . /2
'I"I , N A:
0'
_.....
r,
,
J
,~
to
I., i
"
~ """ " 0
,
~, "~
~o
Ci.G,'
J
~
~r trv' ,,"' e
I-e prP Ho ,
~
2.
\ \ J~J~"
One of the most common methods of treating cancer is using chemical analogues that mim ic th e
structure of nucleotides and prevent DNA polymerase from further binding to DNA during DNA
replication. Explain why this is an effective strategy against cancel' cel ls. /3
rpf" de.'~
e,. lr;>,o"
I ,
r"' l' rJrLt,-".", .
.
,(
. .. .
,.
I J
pra ie,',
f' ' .
.:;, 'rP
I
,..
r
P'" ! '1 ,; f ('
f"
~v t V'
.. i':.
r
1""'
t~
0
I
e
-
"
,"
3.
Describe the steps that would be taken, and identify the processes involved, in making insulin for an
individual with diabetes using recombinant DNA technology (Almost like explaining a procedure) .
You must use a sketch (or sketches) in your explanation. /5
freAe,ri Gr:- BGll" h~
'\
~.~ V Ii,..
· 1~(,v v eve.J
_~e. )-,g ,
fL\
I.:(\...
0
i-~ .. t
r~ ~"~/p!"' L . . ,J
r~U"L
+"-t_
f'r
l kre
CD C-l-~il"~
--n~
III
I
it
" L,
,",, ­0 ',-
oc."",,.t
l .. U
t~ _c-le, ."'­
;J,
o..lJO
"'-U..\1
·h
~ ...
S ..;Y"o(:
;'\G,-,, : (;tot
7V\
to LJ.j.; ,,'JL.. ' ~~ +~ b",c.+@f'" K, .... + 1.,0-_\.0'1'/1,.." s
OfV\£ P, ... ~\ b;Jht 31'.Iedl1'-": , +ke l;$. t"J: oV' Q(
\,,,, de,~ \,,,,-J (.I'.~ 'h. J,,: ,..,. )\\1\.t" ,"" >~ '_/ ,... r. . . 1->1> '--' lee-.
pI (;<"'-"" ,l
e' ~v/"'(
;",f..
b,;\.(..-leY -, '" ~\- r<> vl~ ~ , \'I f+>f 1'\" +u>.,." ,.J,,,
\A 1I
\~ \'" ~ V""c. ~1\{
?( o..5
,,,celie.!
I.f
-1\,,0.. t
Level 2
Levell
Communication
5-
Use of scientific
terminology, sym bol s,
conventions, standard
51 units
I
5.5
-I
6
6
I
6.5
Level 3.
I
Use of scientific
terminology, symbol s,
convention with SOME
accuracy
Use of scientific
terminology, symbols,
conventions with
LIMITED accura cy
2nd base in codon
I
U
C
U C
Pile
Ph{?
leu
leu
Leu
Leu
Leu
leu
Ih"
A
Ser
Ser
Ser
Ser
P-ro
Pro
Pro
Pro
Tnr
A IG
Tyr
C}IS
Tyr
Cys.
STO P
STOP
STOP
His
Arg
Arg
His
Gin
Girl
Trp
I
Arg
Ar!]
Ser
Asn
A
G
U
C
A
G
U
lIe
Thr
Asn
Ser
C
Lys
Val
All!
Ala
Arll
Arll
G1V
~~
Gly
Gly
A
Va]
Vai
Thr
Tllr
Ala
Alii
G I Val
lys
Asp
Asll
GIl-!
Glu
I
The Genetic Code
-
U
C
lie
Met
- -- -- ~ -- -~ -
--
- - -
-7
-­
--­
G
U
C
A
G
7
I
7_5. '
Level 4
I
8
Use of scientific
terminology, symbol s,
conventions with
CONSIDERABLE
accuracy
8.5
J
J
9
I
9.5
Use of scientific
terminology, symbols,
conventions with HIGH
DEGREE of accuracy
I