Oil Production by Coculturing Lipomyces starkeyi & Yarrowia Lipolytica
under Batch-mode Fermentation
Experimental Conditions:
Fermenter used
Working Volume
Wet Biomass of Inoculum
High Cell density concentration
Substrate
Temperature
pH
Agitation
Aeration
Eppendorf Bioflo series 115 (1L)
800 mL
40 g of each yeast strain (Total: 80 g)
100 g/L
5% (w/v) Glucose
Room Temperature
Uncontrolled
200 rpm
Uncontrolled
Fermentation duration
120 hours/5 days
Results:
Glucose & Absorbance profile for Coculturing with Yarrowia Lipolytica &
Lipomyces starkeyii under Oil Production mode
50
25
45
45
38
35
Glucose (g/L)
20
22
17
30
20
19
18
18
15
25
20
10
15
10
5
10
5
0
0
0
20
40
60
Time Interval (h)
Glucose (g/L)
80
0
100
Absorbance
Average glucose consumption rate throughout the fermentation: 9 to 11 g/L/day.
0
120
Absorbance @620nm
40
Microscopic observation for morphology and lipid accumulation (Sudan Black stained)
in Yarrowia Lipolytica & Lipomyces starkeyi during Oil Production:
0 Hour
24 Hours
48 Hours
72 Hours
96 Hours
120 Hours