Reproductive Biotechnology
THER-2701 2(2-0)
Department of Clinical Sciences,
College of Veterinary and Animal Sciences, Jhang
21-09-2020
1
Course Contents
1. Introduction to Biotechnology and its Recent Trends
2. Estrus Cycle, Detection, and Estrus Synchronization
3. Superovulation, and Embryo Transfer
4. Semen Cryopreservation, and Artificial Insemination
5. Sexed Semen
6. Basics of Reproductive Ultrasonography
7. Applications of Reproductive Ultrasonography
21-09-2020
2
Course Contents
8. In Vitro Maturation of oocytes
9. In Vitro Fertilization and Parthenogenesis
10. Embryo Splitting
11. Genetic Engineering
12. Cloning
13. Transgenic Animals
14. Introduction to Stem Cell Therapy
21-09-2020
3
Marks Distribution (Theory)
• Mid Term Exam
12 Marks
• Assignment/Quiz
04 Marks
• Final Term (with some portion of mid)
24 Marks
• Attendance 75% mandatory
• Students participation during class will be highly appreciated
(Two way communication)
21-09-2020
4
Study Sources
• Reproduction in Farm Animals
Seventh Edition. ESE Hafez & B. Hafez
• Controlled Reproduction in Cattle–Buffalo
1st Vol 1. CAB International, UK. Gordon I
• Reproduction in Cattle
By Peter and Ball
• Google Scholar and other search engines
21-09-2020
5
Prerequisite to Understand
• Thorough knowledge of Reproductive physiology
- Sources, target and functions of all hormones
• Application of the knowledge
- For economic benefits
- Increasing production
- Enhancing reproductive efficiency
- Reducing the cost of production and management
21-09-2020
6
Introduction to Biotechnology and its Recent Trends
21-09-2020
7
Theriogenology
• Branch of Veterinary Medicine which deals with reproduction,
- Physiology and pathology of male & female reproductive systems
- Clinical practice of Veterinary Obstetrics, Gynecology, & Semenology
21-09-2020
8
Reproductive Biotechnology
• Biotechnology
The use of living organisms to improve, modify, or produce industrially
important products or processes
• Reproductive Biotechnology
Reproduction-related biotechnologies
Any Examples?
21-09-2020
9
Artificial Insemination (A.I.)
• One of the early reproductive biotechnologies,
• Permits the use of superior males for breeding purpose,
• Semen from superior males is collected and used to inseminate large number of
females,
• Control of the Venereal diseases,
• Endangered species,
• Issues related with transportation and breeding,
21-09-2020
10
Estrus Synchronization
• Bringing the randomly cycling females into one stage of cycle (estrus)
- Prolonging the estrous cycle
- Shortening the estrous cycle
Question: How we can do this?
21-09-2020
11
Superovulation
• Protocol used to make the females develop and ovulate more number of oocytes
in mono-ovulatory animals
- GnRH
- FSH
- PMSG
Ques: Can you name the best source of gonadotropic hormones?
21-09-2020
12
Embryo Transfer (E.T)
• Females with superior genetics are superovulated and their fertilized ova (in vivo
or in vitro) are transferred to recipients,
• Recipients are genetically less distinguished surrogate mothers.
- Surgical
- Non surgical
21-09-2020
13
In-Vitro Fertilization (IVF)
• Fertilization outside the body i.e., in the laboratory,
- Tissue culture medium
- Modified Tyrode’s
- Kreb’s-Ringer’s solution…..supplemented with sources glucose, lactate,
pyruvate and albumin (Ham F-10) supplemented with blood serum
• In-vitro maturation (IVM)
21-09-2020
14
Thanks for your attention
21-09-2020
21-09-2020
15