MANGUNE, CLARK JOSHUA RUTAQUIO
BSP1E
PRACTICE CALCULATIONS
GIVEN:
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Ocular: 10
Lower Power Objective: 5
dFOV (LPO): 3.65 mm
High Power Objective: 50
dFOV (HPO): 4.75n
○ n = object size
SOLUTION:
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REQUIRED:
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Magnification
dFOV (HPO)
Object Size
EQUATION:
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Magnification = Ocular X Objective
(LPO/HPO)
dFOV (HPO) = [Magnification (LPO)
• dFOV (LPO)] / Magnification (HPO)
Object Size = dFOV (HPO) in μm / #
of times object fit across dFOV
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Magnification (LPO) = 10 • 5
○ Magnification (LPO) = 50
Magnification (HPO) = 10 • 50
○ Magnification (HPO) = 500
dFOV (HPO) in μm = [50 • 3.65mm] /
500
○ dFOV (HPO) in μm = 182.5 /
500
○ dFOV (HPO) in μm = 0.365
mm
(1000) convert to
micrometer
○ dFOV (HPO) in μm = 365
μm
Object Size = 365 μm / 4.75 times
○ Object Size = 365 μm / 4.75
○ Object Size = 76.84210526
estimate to the nearest whole
number
○ Object Size = 77 μm
ANSWER:
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77 μm
One cell spans approximately 77
μm.
HANDWRITTEN SOLUTION