write a sientific term 1)is a technique to transfer a gene efficiently and stably into another cell or organism to alter its phenotype. (Gene transfer) 2)A genetic engineering technique to transfer genes using vectors from one organism to another or from one cell to another in order to treat disease, construct GMO and economically important organisms Gene transfer 3) technique agents like live cells, bacteria, other DNA molecules and viruses are used to transmit genes.(Natural Gene Transfer) 4) In this technique, an exogenous gene is transmitted to another cell or organism using artificial means either physical or chemical agents (Artificial Gene Transfer) 5)When gene transfer occurs between two unrelated individuals or organisms, this type of gene transfer (Horizontal Gene Transfer) 6)gene transfer, the genes are transmitted from parents to their offspring.(Vertical Gene Transfer) 7)the transfer of genetic material or genes from one cell to another or one organism to another related organism (Vertical Gene Transfer) 8)gene transfer, the foreign genetic material is never incorporated into the host genome and thus can’t be inherited into offspring.(Transient Gene Transfer) 9)gene transfer, the gene or foreign DNA inserts into the host genome and transmits into the offspring.(Stable Gene Transfer) 10)gene transfer is performed to produce a temporary high level of gene expression and thus it is limited to somatic cells.( Transient Gene Transfer) 11) gene transfer, once the gene is incorporated into the host genome it may express normally, transmit to offspring and express normally in them too. Stable Gene Transfer 12)The formation of electropores depends upon the cells that are used and the amplitude and duration of the electric pulse that is applied to them(Electroporation) 13) can be used to increase efficiency of transformation or transfection of bacterial cells Electroporation 14)The DNA to be transformed into the cells is coated onto microscopic beads made of either gold or tungsten(Biolistic Transformation) 15)A cell without a cell wall or cell membrane (Protoplast) 16)method facilitates direct nuclear delivery of exogenous DNA (Micro-injection) 17) method Using a fine needle, or injection, a DNA or gene is directly injected into the target cell nucleus without disrupting the endogenous pathway ( Micro-injection) 18)r technique is practiced to inject genes directly into oocytes, eggs and embryos Micro-injection 19)Methods may prove useful for gene transfer into cereals which do not regenerate from cultured cell easily (Micro-injection) is a common name used for it.Molecules like DNA, RNA or protein can be inserted into a cell directly Micro-injection a gene of interest coated with heavy metal ions is inserted into the target cell by mechanical force (Gene Gun / Biolistic Trans) 20)spheres of lipids which can be used to transport molecules into the cells. Liposome -Mediated Gene Transfer 21) are artificial vehicles that can act as delivery agents for exogenous materials including transgenes (Liposome -Mediated Gene Transfer) 22)They are considered as sphere of lipid bilayers surrounding the molecule to be transported and promote transport after fusing with the cell membrane Liposome -Mediated Gene Transfer 23)Cationic lipids are t hose having a positive charge are used for the transfer of nucleic acid (Liposome -Mediated Gene Transfer) 24)is the polycationic carbohydrate soluble complex that makes aggregate or complex with the DNA (DEAE dextral -Mediated Gene Transfer) 25)The technique is simple, cost-effective, reliable than other methods and can transfect cells growing into the monolayer (Calcium phosphate Mediated Gene Transfer) 26)it was the first method used to transfect the animal cells (Calcium phosphate Mediated Gene Transfer) 27)method is utilized for protoplast only (Polyethylene Glycol Mediated Gene Transfer) 28)vehicles were less toxic and more resistant to nonspecific protein adsorption making them attractive alternative for nonviral gene delivery ((Polyethylene Glycol Mediated Gene Transfer)