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sentific term lect 7

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1)is a technique to transfer a gene efficiently and stably into another cell or organism to alter its
phenotype.
(Gene transfer)
2)A genetic engineering technique to transfer genes using vectors from one organism to another or from
one cell to another in order to treat disease, construct GMO and economically important organisms
Gene transfer
3) technique agents like live cells, bacteria, other DNA molecules and viruses are
used to transmit genes.(Natural Gene Transfer)
4) In this technique, an exogenous gene is transmitted to another cell or organism using
artificial means either physical or chemical agents (Artificial Gene Transfer)
5)When gene transfer occurs between two unrelated individuals or organisms, this type of gene
transfer (Horizontal Gene Transfer)
6)gene transfer, the genes are transmitted from parents to their offspring.(Vertical Gene Transfer)
7)the transfer of genetic material or genes from one cell to another or one organism to
another related organism (Vertical Gene Transfer)
8)gene transfer, the foreign genetic material is never incorporated into the host genome and thus can’t
be inherited into offspring.(Transient Gene Transfer)
9)gene transfer, the gene or foreign DNA inserts into the host genome and transmits into
the offspring.(Stable Gene Transfer)
10)gene transfer is performed to produce a temporary high level of gene expression and thus it is
limited to somatic cells.( Transient Gene Transfer)
11) gene transfer, once the gene is incorporated into the host genome it may express normally, transmit
to offspring and express normally in them too. Stable Gene Transfer
12)The formation of electropores depends upon the cells that are used and the amplitude and duration
of the electric pulse that is applied to them(Electroporation)
13) can be used to increase efficiency of transformation or transfection of bacterial cells Electroporation
14)The DNA to be transformed into the cells is coated onto microscopic beads made of either gold or
tungsten(Biolistic Transformation)
15)A cell without a cell wall or cell membrane (Protoplast)
16)method facilitates direct nuclear delivery of exogenous DNA (Micro-injection)
17) method Using a fine needle, or injection, a DNA or gene is directly injected into the target cell
nucleus without disrupting the endogenous pathway ( Micro-injection)
18)r technique is practiced to inject genes directly into oocytes, eggs and embryos Micro-injection
19)Methods may prove useful for gene transfer into cereals which do not regenerate from cultured
cell easily (Micro-injection)
is a common name used for it.Molecules like DNA, RNA or protein can be
inserted into a cell directly Micro-injection
a gene of interest coated with heavy metal ions is inserted into the target
cell by mechanical force (Gene Gun / Biolistic Trans)
20)spheres of lipids which can be used to transport molecules into the cells.
Liposome -Mediated Gene Transfer
21) are artificial vehicles that can act as delivery agents for exogenous materials including transgenes
(Liposome -Mediated Gene Transfer)
22)They are considered as sphere of lipid bilayers surrounding the molecule to be transported and
promote transport after fusing with the cell membrane Liposome -Mediated Gene Transfer
23)Cationic lipids are t hose having a positive charge are used for the transfer of nucleic acid
(Liposome -Mediated Gene Transfer)
24)is the polycationic carbohydrate soluble complex that makes aggregate or
complex with the DNA (DEAE dextral -Mediated Gene Transfer)
25)The technique is simple, cost-effective, reliable than other methods and can transfect cells growing
into the monolayer (Calcium phosphate Mediated Gene Transfer)
26)it was the first method used to transfect the animal cells (Calcium phosphate Mediated Gene
Transfer)
27)method is utilized for protoplast only (Polyethylene Glycol Mediated Gene Transfer)
28)vehicles were less toxic and more resistant to nonspecific protein adsorption making them attractive
alternative for nonviral gene delivery ((Polyethylene Glycol Mediated Gene Transfer)
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