Abstract
Objectives
Titanium platelet–rich fibrin (T-PRF), a second-generation autogenous blood concentrate with
tough and thick fibrin meshwork activated by a titanium tube, was used as a drug carrier for
doxycycline (Doxy) by injection. The objective of this study is to evaluate the loading capacity
of T-PRF, release kinetics of doxycycline-loaded T-PRF, and its antibacterial effects against S.
aureus and P. aeruginosa.
Materials and methods
The T-PRF and collagen were loaded with Doxy as T-PRF/Doxy and Collagen/Doxy, and their
release and antibacterial activities against S. aureus and P. aeruginosa were investigated.
Chemical characterization and morphological analysis were performed.
Results
In comparison with collagen, approximately sevenfold more Doxy, 281 mg/g, was loaded into TPRF. It was found that 25% of the loaded Doxy was released from T-PRF compared to only 12%
from collagen within 72 h. The largest inhibition zone diameter (IZD) was observed for TPRF/Dox with 32 ± 6 mm and 37 ± 5 mm for P. aereginosa and S. aureus, respectively.
However, only 10 ± 5 mm and 10 ± 6 mm IZD were observed for bare T-PRF, and no inhibition
zone was observed for the Collagen/Doxy group. A dense fibrin structure was visualized on
SEM images of the T-PRF/Doxy group compared to the T-PRF group.
Conclusions
T-PRF has higher Doxy loading capacity and long-acting antibacterial effects compared to
collagen. T-PRF was shown to have potential autogenous long-term drug-carrying capability for
doxycycline. Also, the potential fibrinophilic properties of Doxy were observed to strengthen the
structure of T-PRF.
Clinical relevance
T-PRF is an autogenous drug career with high loading capacity and extended antibacterial effects
for doxycycline. Doxycycline molecules can be visible on T-PRF fibers. This study suggests that
T-PRF/Dox could be used as a proper antibiotic delivery device in the treatments of periodontitis
and peri-implantitis.
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Acknowledgements
The authors are also grateful to Dr. Süleyman Sami Doğangün and the volunteer pre-graduate
students of Canakkale Onsekiz Mart University Faculty of Dentistry for their valuable
contribution to performing the study.
Funding
The authors thank Canakkale Onsekiz Mart University Scientific Research Council. This Project
was supported by COMU-BAP with Project number TSA-2021–3494.
Author information
Authors and Affiliations
1. Department of Periodontology, Faculty of Dentistry, Canakkale Onsekiz Mart
University, 17110, Canakkale, Turkey
Esra Ercan & Mustafa Tunali
2. Department of Chemistry, Faculty of Sciences & Arts, and Nanoscience and
Technology Research and Application Center (NANORAC), Canakkale Onsekiz
Mart University, Terzioglu Campus, Canakkale, 17100, Turkey
Selin S. Suner, Selehattin Yilmaz & Nurettin Sahiner
3. Department of Pharmacology, Faculty of Medicine, Canakkale Onsekiz Mart
University, Terzioglu Campus, Canakkale, 17100, Turkey
Coskun Silan
4. Department of Biostatistics, Faculty of Medicine, Canakkale Onsekiz Mart
University, Terzioglu Campus, 17100, Canakkale, Turkey
Duygu Siddikoglu
5. Department of Ophthalmology, Morsani College of Medicine, University of South
Florida, Tampa, FL, 33620, USA
Nurettin Sahiner
6. Department of Chemical & Biomedical Engineering, and Materials Science and
Engineering Program, University of South Florida, Tampa, FL, 33620, USA
Nurettin Sahiner
Corresponding author
Correspondence to Esra Ercan.
Ethics declarations
Ethics approval and consent to participate
Approval was obtained from Çanakkale Onsekiz Mart University Clinical Research Ethics
Committee (number: 2020/13; date: November 11, 2020) at the beginning of this study. The
written informed consent was obtained from study participants.
Conflict of interest
The authors declare no competing interests.
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