COMBINE AND CONQUER!
THe process of Dna
recombination and
genetic engineering
For Grade 12
Kent adriane r. pacot
tagum city regional academy for senior high school
Guide Card
We define recombinant DNA technology, alternatively referred to as
genetic engineering, as a technique that enables the combination of
genes in a test tube to produce Hybrid DNA.
Genetic Engineering is primarily used to facilitate the transfer of a desired gene.
Its applications range from improving animal and plant traits, to designing and
manufacturing designer foods and crops, to more advanced applications such
as DNA Fingerprinting and DNA Decoding.
In this material, you will conquer the realm of Genetic
Engineering and become immersed in its intricate processes.
The interactive activities and worksheets are designed to help
you achieve the following objectives:
- Outline the processes involved in Genetic Engineering
- Identify the applications of Recombinant DNA Technology
- Create an infographic explaining the process of Genetic
Engineering
Assessment cards and enrichment activities are also provided to ensure that students learn to their
full potential.
READY UP! this is the time to combine and conquer the realm of dna recombination and
genetic engineering!
ACTIVITY CARD 1
this is the time to combine
In today's time, genetic
engineering has already
influenced our way of life. May it
be our food, livestock and
technology. but do you know
how genetic engineering
works?
Subtask:
COMPLETE THE FLOW CHART THAT
EXPLAINS HOW GENETIC
ENGINEERING WORKS
mATERIALS
Paper
Ball Pen
Worksheet
pROCEDURE
On the next page , answer the
worksheet on how genetic
engineering works
Research and answer the
blank spaces provided
After answering, submit it to
your teacher
ACTIVITY CARD 1
this is the time to combine
ACTIVITY CARD 2
Applications of dna
recombinant technology
Instructions
On the next page, using
the word choices provided
in the boxes, fill in the
numbered boxes with the
steps of bacteria
transformation and the
lettered lines with the name
of the structure next to it.
ACTIVITY CARD 2
Applications of dna
recombinant technology
Assesment CARD 1
Direction: Answer the questions
as neatly as possible
____1. The products of basic genetic processes are (in correct
order)
A. DNA, protein, mRNA
B. DNA, mRNA, protein
C. Protein, mRNA, DNA
D. Protein, DNA, protein
____2. Cinnamon flavor is used in baking apple pies. Now, to
save on adding cinnamon flavoring, you have to insert the
cinnamon flavor gene into apples. Identify the steps you will
undertake.
A. Isolate DNA from apples, clone the apple gene, modify the
apple gene, insert modified gene into cinnamon cells, breed
apple trees
B. Clone the gene from cinnamon tree, modify the cinnamon
gene, insert modified gene into apple cells, breed cinnamon
trees
C. Isolate gene from cinnamon, clone the cinnamon gene,
modify apple gene, insert modified gene into apple trees,
breed apple trees
D. Isolate DNA from cinnamon, clone the cinnamon gene,
modify the cinnamon gene, insert modified cinnamon gene
into apple cells, breed apple trees
_____3. Genome editing tools are versatile methods of genetic
information. These tools could
A. Randomly insert genes into host organism.
B. Insert a whole gene, remove a specific base or bases, alter
a gene, correct a defective gene.
C. Cause the insertion of vector sequences into the host
organism.
D. Knock-out the expression of a target gene
____4. A gene is composed of
A. the transfer of designed genes for a desirable trait
B. crossing of improved varieties or breeds
C. fusion of cells of different taxonomic groups
D. cutting, separation and grafting
____5. The four nucleotides found in the DNA molecule are
A. Alanine, thymine, cytosine, guanine
B. Thymine, adenine, cysteine, guanine
C. Adenine, cytosine, thymine, guanine
D. Thiamine, guanine, alanine, cytosine
____6. Which of the following describe a gene?
A. Unit of heredity
B. A DNA fragment
C. Confers a trait
D. Thiamine, guanine, alanine, cytosine
_____7. The introduction of recombinant DNA into bacterial
cell using a current is called?
A. Transformation
B. Electroporation
C. Transduction
D. Translocation
____8. Which of the following is NOT an argument in favor of
GMOs?
A. Reduced biodiversity
B. Food with extra nutrients
C. Disease-resistant crops
D. Controlled production of insulin
____9. An application of using DNA technology to help
environmental scientists would be
A. use PCR to analyze DNA at a crime scene
B. clone the gene for human growth hormone to treat
pituitary dwarfism
C. create a tobacco plant that glows in the dark
D. make transgenic bacteria that can be used to clean
up oil spills more quickly than do the natural bacteria
_____10. The process of making changes in the DNA code of a
living organism is called
A. selective breeding
B. inbreeding
C. genetic engineering
D. hybridization
Assesment CARD 2
Direction: Analyze the pictures
below and create a synthesis
paper.