Name_____________________________________________________________________________ Period________ Transcription and Translation Practice DNA vs. RNA In the spaces provided, write “D” if the statement is true of DNA. Write “R” if the statement is true of RNA. Write “B” is the statement is true of both DNA and RNA. ______1. consists of a single strand of nucleotides ______2. made of nucleotides linked together ______3. contains deoxyribose ______4. contains the nitrogen base Uracil ______5. contains ribose ______6. is a nucleic acid ______7. consists of a double strand of nucleotides ______8. contains a base that pairs with adenine Complete the table below DNA Number of strands Location Components of nucleotide Function Structure (drawing) mRNA tRNA Match the properties in column A, with the nucleic acids in column B Column A ______9. Column B carries amino acids to ribosomes A. DNA ______10. composed of two chains of nucleotides B. mRNA ______11. contains Thymine C. tRNA ______12. contains ribose D. mRNA and tRNA ______13. involved in translation E. DNA and mRNA ______14. made during transcription F. DNA, mRNA, and tRNA ______15. contains phosphate & ribose ______16. contains Cytosine ______17. contains Uracil Transcription Use the word bank below to label the diagram of transcription: DNA Nitrogen base Breaking H-bond mRNA nucleotide Nucleus Cytoplasm tRNA RNA polymerase 18. Use the word bank above to complete the diagram (not all words will be used) A. __________________ B. __________________ C. __________________ D. __________________ E. __________________ F. __________________ Fill in the blank with correct term or phrase to complete each sentence. 19. In eukaryotes, transcription takes place in the ____________. 20. Transcription begins when ___________________ binds to the gene’s promoter. 21. RNA polymerase adds complementary ________________ as it “reads” the gene. 22. __________ is a strand of nucleotides that carries the DNA message from the nucleus to the ribosomes. 23. ___________ are the part of DNA that is transcribed into mRNA. 24. ___________ are the part of DNA that is not transcribed into mRNA. 25. Transcribe the following DNA strand into its corresponding mRNA strand: DNA: C C A T A G C A C G T T A C A A C G T G A A G G T A A mRNA Translation In the space provided, write the letter of the description that best matches the term or phrase. _____27. ribonucleic acid _____28. uracil A. the entire process by which proteins are made B. a molecule made of linked nucleotides _____29. transcription C. the process of reading instructions on an RNA molecule to put together the amino acids that make up a protein _____30. translation D. the process of transferring a gene’s instructions for making a protein to an RNA molecule _____31. gene expression E. a nitrogen base used in RNA instead of the base Thymine found in DNA 32. Which is the correct sequence? a. DNA RNA protein b. RNA DNA protein c. DNA RNA protein d. RNA DNA protein 33. Put the following events of in the correct order from 1 to 7 _____peptide bonds form between amino acids _____mRNA leaves the nucleus _____DNA unzips _____tRNA molecules base pair with mRNA _____free mRNA nucleotides base pair with DNA _____mRNA reaches the ribosomes _____polypeptide chain folds into a protein 34. What is the name of the process you sequenced above? ___________________________ 35. Complete the set for each of the following table DNA mRNA Amino Acids Fill in the blank: ATG GTA CAA AAT GCG TGT ACT CCC TAG 33. Proteins differ from one another by the order of __________________________. 34. Any change in a nitrogen base sequence is called a _______________________. 35. The function of ____________________ is to carry specific amino acids to the ribosomes. 36. One tRNA molecule carries ______ nitrogenous bases and ______ amino acids. 37. Proteins are linked by ______________________ bonds. Use the word bank below to complete the diagram DNA Anti-codon Codon Ribosome A. __________________ B. __________________ C. __________________ D. __________________ E. __________________ F. __________________ G. __________________ H. __________________ I. __________________ J. ________________ mRNA Amino Acid Nucleus Protein Chain tRNA RNA polymerase Name_________________________________________________________________________________ Period_____ Transcription and Translation Practice KEY DNA vs. RNA In the spaces provided, write “D” if the statement is true of DNA. Write “R” if the statement is true of RNA. Write “B” is the statement is true of both DNA and RNA. R ______1. consists of a single strand of nucleotides B ______2. made of nucleotides linked together D ______3. contains deoxyribose R ______4. contains the nitrogen base Uracil R ______5. contains ribose B ______6. D is a nucleic acid ______7. consists of a double strand of nucleotides B ______8. contains a base that pairs with adenine Complete the table below DNA Number of strands mRNA 1 tRNA 1 Nucleus to cytoplasm cytoplasm Ribose, phosphate, nitrogenous base (G,A,U,C) Ribose, phosphate, nitrogenous base (G,A,U,C) Carries temporary copies of the message contained in DNA to the ribosome to be translated Carry amino acids to the ribosome to create the proper proteins 2 Location Components of nucleotide Nucleus Deoxyribose, phosphate, nitrogenous base (G,A,T,C) Function Stores genetic information that codes for all proteins made in the cell Structure (drawing) Match the properties in column A, with the nucleic acids in column B Column A B ______9. Column B carries amino acids to ribosomes A. DNA A ______10. composed of two chains of nucleotides B. mRNA A ______11. contains Thymine C. tRNA D ______12. contains ribose D. mRNA and tRNA E ______13. involved in translation E. DNA and mRNA B ______14. made during transcription F. DNA, mRNA, and tRNA D ______15. contains phosphate & ribose F ______16. contains Cytosine D ______17. contains Uracil Transcription Use the word bank below to label the diagram of transcription: DNA Nitrogen base Breaking H-bond mRNA nucleotide Nucleus Cytoplasm tRNA RNA polymerase 18. Use the word bank above to complete the diagram (not all will be used) A. ___Nucleus____ B. _Breaking H bond__ C. _RNA polymerase__ D. _Cytoplasm____ E. __mRNA___ F. ____DNA___ Fill in the blank with correct term or phrase to complete each sentence. 26. In eukaryotes, transcription takes place in the ____nucleus___. 27. Transcription begins when ____RNA polymerase___ binds to the gene’s promoter. 28. RNA polymerase adds complementary __nucleotides__ as it “reads” the gene. 29. _mRNA_ is a strand of nucleotides that carries the DNA message from the nucleus to the ribosomes. 30. __Exons__ are the part of DNA that is transcribed into mRNA. 31. __Introns__ are the part of DNA that is not transcribed into mRNA. 32. Transcribe the following DNA strand into its corresponding mRNA strand: DNA: C C A T A G C A C G T T A C A A C G T G A A G G T A A mRNA G G U A U C G U G C A A U G U U G C A C U U C C A U U Translation In the space provided, write the letter of the description that best matches the term or phrase. __B__27. ribonucleic acid __E__28. uracil A. the entire process by which proteins are made B. a molecule made of linked nucleotides __D__29. transcription C. the process of reading instructions on an RNA molecule to put together the amino acids that make up a protein __C__30. translation D. the process of transferring a gene’s instructions for making a protein to an RNA molecule __A__31. Protein synthesis E. a nitrogen base used in RNA instead of the base Thymine found in DNA 32. Which is the correct sequence? a. DNA RNA protein b. RNA DNA protein c. DNA RNA protein d. RNA DNA protein 36. Put the following events of in the correct order from 1 to 7 __6__peptide bonds form between amino acids __3__mRNA leaves the nucleus __1__DNA unzips __5__tRNA molecules base pair with mRNA __2__free mRNA nucleotides base pair with DNA __4__mRNA reaches the ribosomes __7__polypeptide chain folds into a protein 37. What is the name of the process you sequenced above? ___Protein Synthesis___ 38. Complete the set for each of the following table DNA ATG GTA CAA AAT GCG TGT ACT CCC TAG mRNA UAC CAU GUU UUA CGC ACA UGA GGG AUC Amino Acids TYR HIS VAL LEU ARG THR STOP GLY ILE Fill in the blank: 38. Proteins differ from one another by the order of _____amino acids___. 39. Any change in a nitrogen base sequence is called a ___mutation___. 40. The function of ____tRNA__ is to carry specific amino acids to the ribosomes. 41. One tRNA molecule carries __3_ nitrogenous bases and __1__ amino acids. 42. Proteins are linked by __peptide_ bonds. Use the word bank below to complete the diagram DNA Anti-codon Codon A. _____DNA______ B. _RNA polymerase_ C. ____mRNA_____ D. _____Nucleus____ E. _____tRNA______ F. __Protein Chain___ G. _____Ribosome____ H. ____Amino Acid____ I. ____Anti-Codon____ J. _____Codon______ mRNA Amino Acid Nucleus Protein Chain tRNA RNA polymerase Ribosome Name_________________________________________________________________________________ Period_____