MIDDLESEX COMMUNITY COLLEGE MEDICAL LABORATORY TECHNICIAN PROGRAM MLT 201: HEMATOLOGY THEORY AND LAB COURSE SYLLABUS Number of credits: 4.0 3.0 Lecture 1.0 Lab REQUIRED TEXT 1. Clinical Hematology Theory & Procedures, Mary Louise Turgeon, 5th Edition, Lippincott, Williams & Wilkins, 2012. 2. Clinical Hematology Atlas, Bernadette F. Rodak, Jacqueline H. Carr, 4th Edition, Elsevier, 2013. COURSE DESCRIPTION This course is intended to provide the student with a foundation of the theory and principles of hematology in the clinical laboratory setting. Analysis and interpretation of test results as they correlate to clinical diagnosis will be discussed. Hematology Theory and Lab includes a review of general cell morphology with an emphasis on hematopoiesis and bone marrow evaluation. Hematological disease states such as anemias and leukemias are discussed in detail. Hemostasis, thrombosis and disease of the coagulation system are discussed. The course introduces both manual and automated testing in the hematology laboratory. PREREQUISITES: BIO 131, CLS 101, CLS 102 METHOD OF INSTRUCTION Instruction will be provided through lectures, handouts, demonstrations, lab exercises, homework assignments, readings and case studies. METHOD OF EVALUATION Students will be evaluated on their understanding of the course content by a course portfolio which includes assessment of their understanding by multiple measures that include: Written quizzes and exams Laboratory practicums utilizing unknown specimens Assessment of laboratory skills 50% 30% 20% 100% STUDENT LEARNING OUTCOMES Upon successful completion of this course, the student will be able to: 1. Explain the origin of blood cells and articulate the process of erythropoiesis and leukopoiesis as it relates to health and disease. 2. Discuss the coagulation process and its role in maintaining hemostasis. 3. Demonstrate current hematological procedures used to diagnose, monitor and evaluate disorders. 4. Demonstrate the basic principles of hematology instrumentation and the quality assurance and quality control measures used in evaluation. COURSE OBJECTIVES COGNITIVE- With the use of course materials and various teaching methods, the student will be able to: Define hematopoiesis. Describe the collection techniques, preparation and staining of smears, and key terms and concepts as they apply to assessing bone marrow tissue. Define erythropoiesis. Describe and perform standard operational procedures to evaluate erythrocytes and their physical properties using patient blood and quality control samples. Define leukopoiesis. Identify and recognize factors that may alter leukocyte values. List the maturation sequence and identify distinguishing morphology for stages of developing blood granulocytes and agranulocytes. Perform commonly used methods to evaluate these leukocytes. Explain the classification of malignant and nonmalignant leukocytic disorders. Define and identify the clinical signs and symptoms, and hematologic findings of red blood cell disorders. Define hemoglobinopathy and distinguish between qualitative and quantitative hemoglobin defects. Define and list categories associated with neoplastic disorders of leukocytes. Apply general criteria to classify leukemias and identify diagnostic findings on permanently stained blood and bone marrow smears. Define and classify the chronic myeloproliferative and lymphoproliferative disorders by cell type and identification of key morphologic features on permanently stained blood and bone marrow smears. Name disorders based on proliferation of plasma cells and abnormal production of immunoglobulins. List the maturation sequence and identify distinguishing morphology for stages of developing platelets. Define hemostasis, the coagulation process, and fibrinolysis. Define disorders of primary and secondary hemostasis. Define the basic concepts of instrumentation used in the hematology department and the quality assurance and quality control measures utilized for this instrumentation. PSYCHOMOTOR- Given appropriate instruction and all necessary supplies and equipment, the student will perform the following tasks and demonstrate mastery of each task as determined by the instructor. Describe and perform standard operational procedures to evaluate erythrocytes and their physical properties using patient blood and quality control samples. Perform erythrocyte sedimentation rates. Perform standard operational procedures to evaluate reticulocytes and calculations associated with reticulocytes assays. Perform standard operational procedures in peripheral smear examination for red cell morphology. Enumerate and calculate leukocyte cell types and characterize morphologic features used to differentiate developing leukocytes. List the maturation sequence and identify distinguishing morphology for stages of developing blood granulocytes and agranulocytes. Perform commonly used methods to evaluate these leukocytes. Define the basic concepts of instrumentation used in the hematology department and the quality assurance and quality control measures utilized for this instrumentation. AFFECTIVE- Upon receiving appropriate instructions, the student will demonstrate the following attitudes and behaviors at all times as determined by midterm and end term evaluations. Comply with the attendance policy as outlined in the syllabus. Be punctual to class and do not abuse break times. Behave in a safe and professional manner. Adhere to safety regulations while in the laboratory session. Perform at the highest level of standards. Demonstrate interest and enthusiasm for the medical laboratory science profession. Communicate courteously, effectively and professionally with instructors. Demonstrate the ability to get along with others. Maintain a clean and organized work area. Utilize reagents and supplies judiciously. ATTENDANCE Attendance is important and will be taken at each lecture and laboratory session. Students will attend all class meetings, except in cases of reasonable extenuating circumstances. In cases of an emergency, students should contact their instructor (contact information at the beginning of this syllabus). You need to be in class to get assignments, take notes, ask questions and stay involved in the course. You are personally responsible for the assignments and all information missed due to an absence. In the interest of encouraging attendance, I have developed the following policy: Three or more absences (excused or unexcused) will cause your final grade to be lowered by one half of a letter grade (i.e. B to a B-). Each subsequent absence will result in the lowering of the final grade by an additional one half of a grade (i.e. 4 absences = B to a C+). STUDENT SUPPORT SERVICES 1. Academic Alert Program Supports students by connecting them to appropriate campus services if they are experiencing any academic difficulties in class. If I notice that you are experiencing any difficulties in class such as excessive absence or consistent tardiness, I will send you an email through the Academic Alert Program to your Middlesex email account. The email will tell you about my concerns for your academic progress in class and will refer you to a counselor in a campus support service department who can help you to address the academic difficulties that you are experiencing and work with you to complete a Strategies for Success Plan. The early alert emails will only be sent to your Middlesex emails accounts so please check the account frequently. If you do not know how to access you MCC email account, contact the Help Desk at 781-2803757 or email them at Helpdesk@middlesex.mass.edu. 2. Supplemental Instruction Supplemental Instruction is an academic support program that uses model students to attend scheduled classes and facilitate voluntary study groups outside the classroom. In the SI sessions, students: integrate content and learning skills combine “How to learn” with “what to learn” use collaborative learning strategies to create an active learning environment LECTURE TOPICS Origin of blood cells Erythrocyte maturation, physiology, and lifecycle Erythrocyte morphology and inclusions Classification and laboratory assessment of anemias Hemoglobinopathies and thalassemias Granulocytic leukocyte series Monocytic-macrophage series Lymphocytic and plasma cell series Nonmalignant disorders of leukocytes Leukemias, lymphomas and myelomas Lymphoid and plasma cell neoplasms Myeloproliferative neoplasms Principles and disorders of hemostasis and thrombosis Molecular diagnostics in the hematology lab LABORATORY TOPICS Safety in the clinical laboratory Quality control and quality assurance Manual red blood cell counts Manual hemoglobin determinations Microhematocrit testing RBC indices Manual white blood cell counts Slide preparation and staining RBC morphology White blood cell identification Normal and abnormal WBC differentials Reticulocyte counts Erythrocyte sedimentation rate Hematology instrumentation Basic coagulation studies, PTINR, PTT, D-Dimer, Fibrinogen This work is licensed under a Creative Commons 3.0 License http://creativecommons.org/licenses/by/3.0 This workforce solution is 100% funded by a grant awarded by the U.S. Department of Labor, Employment and Training Administration, TAACCCT grant agreement # TC-22505-11-60-A25.The solution was created by the grantee and does not necessarily reflect the official position of the U.S. Department of Labor. 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