Female rat - liver Section 1 - Group 1 Positive Negative DAPI α-VCAM-1 Merge 40X Female rat - kidney Section 1 - Group 2 Positive Negative DAPI α-VCAM-1 Merge 40X Female rat - heart Section 1 - Group 3 Positive Negative DAPI α-VCAM-1 Merge 40X Female OVX* rat – liver *OVX= ovariectomised Section 1 - Group 4 Positive Negative DAPI α-VCAM-1 Merge 40X Female OVX* rat – heart *OVX= ovariectomised Section 1 - Group 5 Positive Negative DAPI α-VCAM-1 Merge 40X Female OVX* rat – heart *OVX= ovariectomised Section 1 - Group 6 Positive Negative DAPI α-VCAM-1 Merge 40X EXAMPLE 40X Mouse heart α-SMA Ab. (2⁰ Ab FITC) Staining DAPI as counter stain Mouse heart Negative staining