Extraction of Powdered Pineapple (Ananas comosus) from Pulp, Pomace and its Utilization in Product Development A Project Report submitted in partial fulfilment of the requirements for the award of the degree of BACHELOR OF TECHNOLOGY in FOOD ENGINEERING AND TECHNOLOGY By HIMONABH MAHANTA (FPB17025) VEDANTA MADHAV SHIVAM (FPB17027) UNDER THE GUIDANCE OF PROF. Dr. SANKAR CHANDRA DEKA Sir DEPARTMENT OF FOOD ENGINEERING AND TECHNOLOGY TEZPUR UNIVERSITY TEZPUR-784028 (ASSAM) ABSTRACT Currently, considerable emphasis has been given on the study of various health beneficial properties of locally available products and a to develop products that can be available to major part of the consumers. The pineapple plant or locally known as ‘Anaros’ in Assam is an important fruit grown successfully in tropical climates. Parts of the pineapple fruit including the pulp, are used in various foods. The pineapple cookies made from the pulp and the pomace were obtained from locally grown variety of pineapple from Assam. Two methods were applied to the pineapple pulp and pomace namely drying and powdering. The pineapple pulp and pomace were dried after cleaning using Tray Drying at 55’C to 60’C for 12-14 hours. Pineapple flavoured cookies was prepared from the pulp and pomace and was stored at room temperature in air tight containers. Proper sensory analysis was also performed. Significant similarities can be seen between commercially available cookies and Pineapple pulp and pomace cookies. Keywords: Pineapple, pulp, pomace, bromelain, cookies, tray drying, hedonic. TABLE OF CONTENTS 1 Introduction 2-9 1.1 Significance of study 1.2 Objective 1.3 Plan of work 2 Review of literature 2.1 Pineapple Plant 2.2 Nutritional Quality 2.3 Constituents of Pineapple 2.4 Uses of Pineapple 10 - 11 3 Materials and Methods 12 - 19 3.1 Raw Material Collection 3.2 Cleaning/ Pretreatment 3.3 Crushing 3.4 Drying 3.4.1 Tray drying 3.5 Powdering 3.6 Storage 3.7 Applications/ Utilization in product development 3.7.1 Preparation 3.7.2 Baking 3.7.3 Using powdered pineapple pulp to make Pineapple Flavored Cookies 3.8 Sensory Analysis (Hedonic test) 4 Summary & Conclusion 5 Bibliography 20 - 21 LIST OF FIGURES Fig No. Figures Page No. 1 12 4 (a) Pineapple fruits peeled and ready Cleaned and then cut into smaller similar sized pieces Required amount crushed into pomace and juice extracted Sun-dried pineapple pulp 4 (b) Sun-dried pineapple pomace 14 5 (a) 15 8 Sun drying the pineapple pulp Sun drying the pineapple pomace Making into powder the pineapple pulp Making into powder the pineapple pomace Storage of powdered pineapple pulp in airtight containers Storage of powdered pineapple pomace in airtight containers Electric convection oven 9 Prepared dough 21 10 Prepared cookies before baking Final cookies 22 2 3 5 (b) 6 (a) 6 (b) 7 (a) 7 (b) 11 ~1~ 13 13 14 16 16 17 17 18 20 22 CHAPTER - I INTRODUCTION: Fruits are generally associated with a structure that resembling a fleshy seed which is often processed before consumption or can be consumed raw. The fruit flesh can be sweet, sour or in some cases even bitter. A diet that is rich in fruits has always been encouraged in people's lifestyles because fruits have plenty of health benefits and the list is seemingly endless. It is well known that due to their nutritional qualities, the daily intake of fruits also helps to avoid certain diseases like Alzheimer’s disease, cancer etc. Some fruit rich diets also help to fight against and reduce the risk of high blood pressure, heart diseases, cholesterol, diabetes etc. There are many fruits in the market available such as mangoes, apples, kiwis, jackfruits, guavas, pears, oranges, pineapples and many others and each of them have their own health benefits. They enrich our body with minerals in some or the other kind of way. Drying of parts of plant is an important industrial process that involves the removal of moisture from a wet solid by means of facilitated heat and mass transfer. It is a very common method to store it for a long period of time and it is a very economical one. Dried products can be consumed and also stored for a long time thus increasing its shelf life. Some of the benefits of drying include no requirement of freezing and chilling, easy to prepare, high shelf life etc. But during most of the common drying processes there can be loss of nutrients, volatile and bioactive compounds. Food powders are ever-present in everyday life in a range of applications (e.g. salt, pepper, spices, sugar, flour, coffee, almond powder, dry beverages, cakes, ice cream mixes, colouring agents, etc.). They also provide technological solutions, since they are relatively easy to preserve, transport, store and process. Although significant scientific research has been conducted over the past 15 years, descriptions of food powders still remain partial (BarbosaCanovas et al., 2005; Cuq et al., 2011; Fitzpatrick and Ahrné, 2005). A global powder engineering approach is required to answer the requirements of the production and usages of powders by integrating disciplines related to food science and technology with academic disciplines such as process engineering, chemistry or physics. Understanding the behaviour of granular matter constituted by dense assemblies of solid heterogeneous grains under ~2~ external stresses remains highly relevant. Food powders represent a large proportion of the total processed food in the world. There are several reasons for this, such as low bulk weight; storage, transport and usage conveniences; diverse applications; relatively high stability; and the possibility of a high production rate. Most food powders have low moisture content, thus, reducing the rate of quality degradation. Hence, food powders can be stored for a longer time than other forms of food products. However, quality degradation in food powders may occur with or without changing physical appearance depending on their chemical composition and physical states. Quality degradation of food powders mostly involves both chemical and physical deteriorations and, at times, both of these changes are linked. Pineapple (Ananas comosus) belongs to the family Bromeliaceae, locally called 'anarosh' in Assam is a tropical plant with an edible fruit and the most economically significant plant in the family Bromeliaceae. The pineapple is indigenous to South America. 100 g of pineapple contain 47-52 calories, 85.3-87.0 g water, 0.4-0.7 g protein, 0.2-0.3 g fat, 11.6-13.7 g total carbohydrate, 0.4-0.5 g fibre, 0.3-0.4 g ash, 17-18 mg calcium, 8-12 mg phosphorus, 0.5 mg iron, 1-2 mg sodium and 125-146 mg potassium. Pineapple also contains 12-15 % sugars of which two-third is in the form of sucrose and the rest are glucose and fructose. 0.6-1.2 % of pineapple is acid of which 87 % is citric acid and 13 % is malic acid. The pH of pineapple is acidic, which is 3.71 and the acidity percentage is 53.5 %. The composition of the juice varies with geographical, cultural and seasonal harvesting and processing. Pineapples grow as a small shrub and the individual flowers of the unpollinated plant fuse to form a multiple fruit. The plant is normally propagated from the offset produced at the top of the fruit, or from a side shoot, and typically mature within a year. The plant has 30 to 40 stiff succulent leaves closely spaced in a rosette on a thick fleshy stem. In commercial varieties about 15 to 20 months after planting, a determinate inflorescence forms on a flower stalk 100–150 mm (4–6 inches) in length. The originally separate light purple flowers, together with their bracts, each attached to a central axis core, become fleshy and fuse to form the pineapple fruit, which ripens five to six months after flowering begins. Fruits of commercial varieties range from 1 to 2 kg in weight. The caloric content of pineapples is low, reason why it is suitable for slimming diets. It helps to digest and beneficial for people with gastritis or dyspepsia. It is a slightly laxative fruit and it regularizes the intestinal functions. This fruit activates the pancreatic function. The edible flesh is surrounded by green bracts that turn into orange when ripe, which form the skin of the fruit. The bracts are clustered in the upper part of the fruit forming a tuft of leaves. Pineapples have yellow or white pulp, fleshy, aromatic, ~3~ juicy and sweet. In the fruit there is a fibrous axis that extends from the crown to the pedicle. Mature pineapples have a very singular fragrance, a beautiful colour and pleasant bitter-sweet taste. It is eaten raw or as an ingredient in juices, preserves, liquors, etc. Both the fruit and the leaves are used to prepare medicinal compounds. The pineapple is an herbaceous plant whose inflorescences are born in the tip of the fruit. These inflorescences have an oval shape. It is a multiple fruit composed of a clustering of fruits. It is a fleshy fruit ending in a crown of leaves. Pineapples has a very high content of water. The caloric value, taking the composition into account, is very low. Each 100 g of edible fresh product supply between 64 and 101 kcal. For that reason, pineapples are suitable in slimming diets. This fruit is very sensitive to sudden changes of temperature. The suitable temperatures for partially mature pineapples are around 10-13ºC and for mature pineapples around 7-10ºC. Thus, the optimal levels of relative moisture are between 85 and 90%. Drying as a method of preservation has numerous advantages. Food becomes lightweight and easy to carry due to the reduced water content in it. A research conducted by Dr. Naseer Ahmed, of RIMT University, says, "Dried foods are tasty, nutritious, lightweight, easy-toprepare, and easy-to-store and use. The energy input is less than what is needed to freeze or can, and the storage space is minimal compared with that needed for canning jars and freeze containers. The nutritional value of food is only minimally affected by drying. In tray dryers, the food is spread out, generally quite thinly, on trays in which the drying takes place. Heating may be by an air current sweeping across the trays, by conduction from heated trays or heated shelves on which the trays lie, or by radiation from heated surfaces. Most tray dryers are heated by air, which also removes the moist vapours. A tray dryer is a convectional drying equipment with enclosed insulated chambers and trays placed on top of each other in a trolley. The driers are used in processes where drying and heating are a crucial part of the industrial manufacturing process such as in the food products, pharmaceutical, and chemicals manufacturing among others. The materials to be dried are either solid or wet and are placed on the trays. The heat through the trays is transferred through hot air circulation by a stream in a radiator coil or electric heater. The transfer of heat, as well as the proper circulation of the heat, is facilitated by the installed blower fans. Additionally, the equipment has a control panel for temperature control as well as other parameters fixed outside the equipment and assist in its functioning. The basic working principle of this machine is the continuous circulation of hot air. In the tray dryer, moisture is removed from the solids that are placed in the tray by a forced convectional heating. The moist air is removal is conducted partially but ~4~ in a simultaneous fashion. Initially, the wet solids are loaded into the trays and the trays placed in the dryer chambers. There is then the introduction of fresh air through the inlet, the air passes through the heaters where it is heated up. The objective of the heating is to ensure that the heated air reaches the wet solids on the tray, therefore, the fans in the tray dryer are used to circulate the hot air at a speed of two to five meters per second. The turbulent air flow reduces atmospheric partial vapor pressure and the thickness of the layers of the air boundary. The heated air picks up water. During water evaporation from the surface, there is diffusion of the water from the interior of the solids by a capillary action. All the above-mentioned events happen in a single pass of air. There is a short contact time and only a small amount of water is picked in a single pass and as a result, the discharge air to the rate of 80 to 90 percent is circulated back through the fans and only a maximum of 20 percent of fresh air is introduced. The moisture discharge occurs through the outlet. Therefore, uniform air flow and constant temperature over the solid materials can be maintained for achieving uniform drying. In the event the materials to be dried are wet granules such as in capsule or tablet drying, drying is continued until the desired moisture content is attained. At the completion of the drying process, the drying trucks or trays are pulled out of the chamber and taken to the tray dumping station. In totality, the large working space, sturdy construction, controlled temperature, uniform air circulation are the basic principles for the functionality of the tray dryer. it is an equipment with an ability to assist in the drying of plastic and chemical powder, electrodes, bakery, food, among other equipment. To avoid nutritional losses, pretreatments are given to the raw materials before drying. Pretreatments can fix color loss, enzymatic reaction and loss of nutrients and other health beneficial compounds. Pretreatment includes any method of treating the product prior to freezing. This may include concentrating the product, formulation revision (i.e., addition of components to increase stability, preserve appearance, and/or improve processing), decreasing a high-vapor-pressure solvent, or increasing the surface area. Pineapples are an important source of sugars, organic acids (citric acid), essential minerals (Cu, Mg, Mn, K), fiber and vitamins (A, C, B‐group) for human nutrition. One way through which the pineapple fruit help us in countless ways is through the enzymes that are present in them. Bromelain is a mixture of proteolytic enzymes present in pineapple. It is present in all parts of the pineapple plant. If having sufficient bromelain content, raw pineapple juice may be useful as a meat marinade and tenderizer. Although pineapple enzymes can interfere with ~5~ the preparation of some foods or manufactured products, such as gelatin-based desserts or gel capsules, their proteolytic activity responsible for such properties may be degraded during cooking and canning. The quantity of bromelain in a typical serving of pineapple fruit is probably not significant, but specific extraction can yield sufficient quantities for domestic and industrial processing. ~6~ 1.1. Significance of the Study: Pineapple plant (Ananus comosus, Bromeliaceae) is useful in many ways including immense health benefits as well. Pineapple contains considerable calcium, potassium, fibre, and vitamin C. It is low in fat and cholesterol. Vitamin C is the body's primary water soluble antioxidant, against free radicals that attack and damage normal cells. It is also a good source of vitamin B1, vitamin B6, copper and dietary fibre. Pineapple is a digestive aid and a natural Anti-Inflammatory fruit. Bromelain that is a group of sulfurcontaining proteolytic enzymes in pineapple aid in better digestion. Fresh pineapples are rich in bromelain and can also be used for tenderizing meat. Bromelain has demonstrated significant anti-inflammatory effects, reducing swelling in inflammatory conditions such as acute sinusitis, sore throat, arthritis and gout and speeding recovery from injuries and surgery. Pineapple enzymes have been used with success to treat rheumatoid arthritis and to speed tissue repair as a result of injuries, diabetic ulcers and general surgery. Pineapple reduces blood clotting and helps remove plaque from arterial walls. Studies suggest that pineapple enzymes may improve circulation in those with narrowed arteries, such as angina sufferers. Pineapples are used to help cure bronchitis and throat infections. It is efficient in the treatment of arteriosclerosis and anaemia. Pineapple is also an excellent cerebral toner that is it combats loss of memory, depression and melancholy. Pineapple fruits are primarily used in three segments, namely, fresh fruit, canning and juice concentrate with characteristic requirements of size, shape, colour, aroma and flavour. Bromelain is a complex mixture of substances that is present in the pineapple plant including the stem, crown and the fruit core. Among dozens of components known to exist in this crude extract, the best studied components are a group of protein-digesting enzymes (called cysteine proteinases). Originally, it was believed that these enzymes provided the key health benefits found in bromelain, a popular dietary supplement containing these pineapple extracts. In addition, researchers believed that these benefits were primarily limited to help with digestion in the intestinal tract. However, further studies have shown that bromelain has a wide variety of health benefits, and that many of these benefits may not be related to the different enzymes found in this extract. Excessive inflammation, excessive coagulation of the blood, and certain types of tumor growth may all be reduced by therapeutic doses of bromelain when taken as a dietary ~7~ supplement. Studies are not available, however, to show these same potential benefits in relationship to normal intake of pineapple within a normal meal plan. It is also suspected that it will eventually turn out to show some unique health-supportive properties, including possible digestion-related and anti- inflammatory benefits. Some of its reported actions include inhibition of platelet aggregation, anti-edematous, antithrombotic, anti-inflammatory, modulation of cytokines and immunity, skin debridement and fibrinolytic activity. It also assists digestion, enhance absorption of other drugs and is a potential postoperatively agent that promote wound healing and reduce postsurgical discomfort and swelling. and crown etc. Extraction of bromelain from parts such as the peel, stem or the crown is attractive not only from environmental point of view but also economically (Novaes et al. 2013). With these ample amount of health benefits that the Pineapple has to offer, our approach in the present study was to obtain powdered Pineapple pulp once using the pomace and another from fresh Pineapple pulp. The objective was to properly powder the two and via the utilization of proper materials and methods obtain the powdered form. Also, the utilization of the powder in product development such as cookie, biscuit making that can aid in some manner is also of prime significance to this study. ~8~ 1.2. Objectives: The objectives of our study are as follows: I) To make Pineapple flavoured cookies fortified with natural powdered Pineapple extracts. II) To study the sensory and laboratory based analysis of the cookie and the powdered Pineapple extract. ~9~ 1.3. PLAN OF WORK Pineapple Cleaning/ Pretreatment Cutting Pulp Pomace Tray drying Tray drying Powdered Fortification into cookie dough Baked Cookies Figure: Plan of Work ~ 10 ~ CHAPTER - II REVIEW OF LITERATURE: 1.1 Pineapple Plant: The pineapple (Ananas comosus) is a tropical plant with an edible fruit and the most economically significant plant in the family Bromeliaceae. The adult plant is 1–2 m high and 1–2 m wide, and has the general shape of a spinning top. The main morphological structures to be distinguished are the stem, the leaves, the peduncle, the multiple fruit or syncarp, the crown, the shoots and the roots. The fruit is usually parthenocarpic. The cultivated fruits are seedless. The fruit pulp is white to yellow with a sourish-sweet, aromatic smell and taste. The following description is mainly focused on the cultivated pineapple and is partly based on the anatomical studies of Krauss and Okimoto (1948). Pineapple is a source of bromelain, used as a meat-tenderizing enzyme and high quality fibre. The waste resulting from industrial processing is often used for animal feed. Pineapple is cultivated predominantly for its fruit that is consumed fresh or canned. Even, the stems and leaves of the pineapple plant can be used as a source of fibre, which can be processed into paper. 1.2 Nutritional Quality: The fruit is a good source of manganese and contains significant amounts of vitamins C and B1. Pineapple contains the proteolytic enzyme bromelain, which is used as a meattenderising agent and for medicinal purposes. It has been reported to have valuable biological properties such as interfering with the growth of malignant cells, inhibiting platelet aggregation, fibrinolytic and anti-inflammatory action, enhancing drug absorption and removing skin (debridement) (Gailhofer et al. 1998; Mynott et al. 1999; Hale et al. 2005). Pineapple leaf juice is used as a purgative (agent that cleanses the bowel), emmenagogue (agent that induces menstrual bleeding) and vermifuge (agent that expels intestinal worms) (Leal & Coppens d'Eeckenbrugge 1996; Coppens d'Eeckenbrugge & Leal 2001). Proteolytic digestive: Bromelain is a digestive ferment comparable to pepsin and papain. The plant also acts as an anti-inflammatory, hypolipidemic, antiplatelet. It is diuretic, high vitamin content and has nutritional value. It is also a dissemination agent, and a detergent for sores and wounds. Antineoplastic effects have been observed as well as an elevation of the serum level ~ 11 ~ of antibiotics when administered concurrently. The fruit is used for dyspeptic symptoms, rheumatism, arthritis, gout, urolithiasis, arteriosclerosis, bronchitis, emphysema, asthma, cystic fibrosis. There is also topical use associated with pineapple plant that includes cleaning wounds and trophic ulcers. The pineapple core is attributed as an adjuvant in weight-loss diets, because of its high fiber content with satiating action and slightly laxative action. 1.3 Constituents of Pineapple Plant: The active ingredients of pineapple include proteolytic enzymes (bromelain); citric and malic acids; vitamins A, B and C; sugars such as glucose, sucrose; fats; mineral salts like iodine, magnesium, manganese, potassium, calcium, phosphorus, iron, sulfur; and high fiber (cellulose) in the core of the fruit. 1.4 Uses of Pineapple: Traditional uses of pineapple include the brewing of pineapple wine, production of fiber, and medicinal use to induce menstruation, induce abortion, kill parasitic amoebas, and expel worms. Pineapple has proteolytic enzymes known as bromelain. Bromelain has been known for its vast commercial applications. It is being used in food and beverage industries (Neta et al. 2012), in meat tenderization, in cosmetic industries (Orsini 2006) as well as in textile industries (Arshad et al. 2014). Besides its industrial applications, bromelain possess multiple therapeutic actions. Some of its reported actions include inhibition of platelet aggregation, anti-edema (MacKay et al. 2003), antithrombotic and fibrinolytic activity (Errasti et al. 2016), anti-inflammatory action (Brien et al. 2004), anti-tumor action (Dhandayuthapani et al. 2012; Pillai et al. 2013), modulation of cytokines and immunity, skin debridement properties (Hu et al. 2011; Rosenberg et al. 2012), enhanced absorption of other drugs (Orsini 2006), mucolytic properties, digestive assistance, enhanced wound healing (Taussig et al. 1988), cardiovascular and circulatory improvement (Kavitha et al. 2013). ~ 12 ~ CHAPTER - III MATERIALS AND METHODS: 3.1. Raw Material: Pineapple (Ananas comosus) pulp is mainly used in this work. The pineapple for this study was obtained fresh and locally from the nearby farmer's market in Beltola, Guwahati. Figure 1: Pineapple fruits peeled and ready. 3.2. Cleaning/ Pretreatment: Prior to further processing the pineapple pulp is washed with running water and excess water is drained out in addition to removal of the dust particles, sand and other foreign or extraneous matter from the fruit part. This pretreatment is fundamental to guarantee the useful life of the raw materials or achieve better performance in operations. This also involved cutting the fruit pulp into smaller pieces in order to ensure that all of them were evenly dried. ~ 13 ~ Figure 2: Cleaned and then cut into smaller similar sized pieces. 3.3 Crushing: For obtaining the pineapple pomace, we crushed few of the sliced pineapple pieces to extract the juices and obtain the pineapple pomace. Figure 3: Required amount crushed into pomace and juice extracted ~ 14 ~ 3.4. Drying: If we want to obtain the powdered form, first, we have to go for drying. However as conventional drying methods are sometimes destructive in nature and may disturb the important constituents therefore, the traditional method of sun drying is a preferable method of drying in these circumstances. Figure 4 (a): Tray-dried pineapple pulp. Figure 4 (b): Tray-dried pineapple pomace. ~ 15 ~ 3.4.1. Tray Drying: The methods involved in this drying process are elaborated below -i) Ideally, the fruit are cut into similar-sized pieces in order to ensure that all of them are evenly dried ii) We used good drying techniques and used separate trays to spread out the fruit in the tray. The trays were made of stainless steel. iii) We placed the drying trays in the dryer. Stacking them according to preference. iv) Normally, it took 12 hours at 60 o Celsius to dry the fruits accordingly. We employed the best practice of letting them sit through the day and bought them back in the night. Figure 5 (a): Tray drying the pineapple pulp and pomace. ~ 16 ~ Figure 5 (b): Tray Dryer 3.5. Powdering: The sun-dried pineapple pulp and the pomace was then grinded into a powdered form using a mini mixer until the desired powdered form was obtained. Figure 6 (a): Making into powder the pineapple pulp ~ 17 ~ Figure 6 (b): Making into powder the pineapple pomace 3.6. Storage: The crude powdered form obtained from the above steps was then stored in airtight containers for further analysis. Figure 7 (a): Storage of powdered pineapple pulp in airtight containers ~ 18 ~ Figure 7 (b): Storage of powdered pineapple pomace in airtight containers 3.7. Applications/ Utilization in product development: Pineapple has been known for its vast commercial applications. It is being used in food and beverage industries (Neta et al. 2012), in meat tenderization, in cosmetic industries (Orsini 2006) as well as in textile industries (Arshad et al. 2014). Besides its industrial applications, bromelain possess multiple therapeutic actions. Some of its reported actions include inhibition of platelet aggregation, anti-edema (MacKay et al. 2003), antithrombotic and fibrinolytic activity (Errasti et al. 2016), anti-inflammatory action (Brien et al. 2004), antitumor action (Dhandayuthapani et al. 2012; Pillai et al. 2013), modulation of cytokines and immunity, skin debridement properties (Hu et al. 2011; Rosenberg et al. 2012), enhanced absorption of other drugs (Orsini 2006), mucolytic properties, digestive assistance, enhanced wound healing (Taussig et al. 1988), cardiovascular and circulatory improvement (Kavitha et al. 2013). ~ 19 ~ 3.7.1. Using powdered pineapple pulp to make Pineapple Flavored Cookies: Ingredients used for the cookie preparation: Sugar-60g Flour-130g Butter-100g Pineapple Powder-32.5g (25% of Flour) Pineapple Essence-1tsp Baking Powder- 1/2 tsp Salt-pinchwise Dalda-10g Recipe: 1. Mix butter in sugar. 2. Wisk it. 3. Now add essence of pineapple and powder. 4. Add flour and mix it. 5. Give it some shape. 6. Bake it at 180oC for 10 minutes. Figure 8 : Electric Convection Oven ~ 20 ~ Figure 9: Prepared Dough Figure 10: Prepared cookies before baking ~ 21 ~ Figure 11: Baked Cookies Figure 12: Final cookies Sensory analysis: We choose a commonly-used affective method that include the hedonic test method because the test method must be simple and easy to understand, so the consumers making up the panel will know how to respond The hedonic scale may be used to determine degree of acceptability of one or more products. This scale is a category-type scale with an odd number (five to nine) categories ranging from “dislike extremely” to “like extremely.” A neutral midpoint (neither like nor dislike) is included. Consumers rate the product on the scale based on their response. 9 Point Hedonic Scale: Like extremely (9) Like very much (8) Like moderately (7) Like slightly (6) ~ 22 ~ Neither like nor dislike (5) Dislike slightly (4) Dislike moderately (3) Dislike very much (2) Dislike extremely (1) Serial No Sample1 Sample2 Names 1 8 9 Rishabh Trivedi (Btech, Tezpur University) 2 8 8 Bhupendra Das (Btech, Tezpur University) 3 7 7 Biswa Jyoti Neog (Btech, Tezpur University) 4 6 8 Dipesh Das (Btech, Tezpur University) 5 7 6 Ripunjoy Bordoloi Pamchong (Btech, Tezpur University) 6 7 6 Utkarsh Tiwari (Btech, Tezpur University) ~ 23 ~ CHAPTER - IV SUMMARY AND CONCLUSION: A study was conducted on the pulp and pomace and applications of Pineapple (Ananas comosus) plant. Upon studying its various health beneficial properties and having a culinary significance, pineapple flavoured cookies fortified was prepared from their pulp and pomace. Trials were done based on their sensory characteristics to achieve the desirable blend of ingredients. The pulp and the pomace is extracted from Pineapples locally grown variety from Assam. Two methods were applied namely tray drying and baking. Cookie was prepared from pulp and pomace and sensory characteristics were analyzed. From all the analysis that have been studied it was found that the cookies received average ratings as per the hedonic scale. With some people giving it a 9 point as they found the cookie really delicious. Pineapple is considered as one of the most important fruit of India and its flavour have also attracted the taste buds of people worldwide. It has various health beneficial properties and helps in refreshing the mind and body. Today there are numerous usage of pineapples and is used in many culinary dishes because of their health beneficial properties. ~ 24 ~ CHAPTER - V BIBLIOGRAPHY: Department of Health and Ageing. 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