HPLC Columns
Capture the Essence
2013 – 2015
SHOWA DENKO K.K.
TM
We provide a wide range of products to meet your analytical needs, from pretreatment and
separation columns to calibration standards for size exclusion chromatography.
Please visit the Shodex website to see application data and other useful info.
Shodex website
www.shodex.de
The following names are trademarks or registered trademarks of SHOWA DENKO K.K.
Shodex, AFpak, Asahipak, AXpak, CLNpak, CXpak, MSpak, ODP, ODSpak, OHpak, ORpak, RSpak, Silicapak,
SUGAR, USPpak
[Caution]
1. Please read the operating manual included in the product box carefully before use.
2. For improvement purposes, some specifications might change without notice.
3. The figures and descriptions in this catalogue are not guaranteed and do not warrant suitability for your applications.
4. It is essential to take normal precautions when handling reagents and other chemical products even if the safety
information is not included in the operating manual.
5. Products described in this brochure are not intended for medical use or medical applications
including medical diagnosis.
Contents
Column Selection
Types of Columns, Base Materials, Functional Groups and Ligands
HPLC Separation Modes
Column Selection by Sample Character and Separation Mode
Column Selection (Application)
2
3
3
4
Standard Analysis Columns
Reversed Phase,
Hydrophilic Interaction and
Normal Phase Chromatography
Ligand Exchange Chromatography
Ion Exclusion Chromatography
Ion Chromatography
Size Exclusion Chromatography
Calibration Standards
for SEC
Ion Exchange Chromatography
Columns for Polymer-based Reversed Phase Chromatography
Columns for Polymer-based Hydrophilic Interaction Chromatography (HILIC)
Columns for Silica-based Reversed Phase, HILIC and Normal Phase Chromatography
Columns for Ligand Exchange Chromatography
Columns for Ion Exclusion Chromatography
Columns for Ion Chromatography (Anion Analysis)
Columns for Ion Chromatography (Cation Analysis)
Column Selection for Size Exclusion Chromatography (SEC)
Precautions for Polar Polymer Analysis
Aqueous SEC (GFC) Columns : Silica-based
Aqueous SEC (GFC) Columns : Polymer-based
Multimode Columns
Aqueous/Organic SEC Columns
Organic SEC (GPC) Columns (General Analysis) : THF
Organic SEC (GPC) Columns (General Analysis) : Chloroform
Organic SEC (GPC) Columns (General Analysis) : DMF
Solvent-peak Separation Columns for Organic SEC (GPC)
Organic SEC (GPC) Columns : Rapid Analysis
Organic SEC (GPC) Columns : High Performance Analysis
Organic SEC (GPC) Columns : Linear Calibration Type
Organic SEC (GPC) Columns : High Temperature/Ultra High Temperature Analysis
Organic SEC (GPC) Columns : HFIP
Applicability of SEC (GPC) Columns to Solvent Replacement
Calibration Standards for SEC
Columns for Anion Exchange Chromatography
Columns for Cation Exchange Chromatography
6
12
14
16
20
22
24
26
27
28
30
32
34
36
38
40
40
42
42
44
46
48
50
51
Columns for Polymer-based Reversed Phase Chromatography
Columns for Polymer-based Hydrophilic Interaction Chromatography (HILIC)
Aqueous/Organic SEC Columns
Organic SEC (GPC) Columns
52
54
56
56
56
56
58
58
58
58
Columns for GPC Clean-up
Pretreatment Columns for Column Switching Method
62
64
Pharmaceuticals and Cosmetics Analysis
Foods Analysis
Biotechnology Analysis
Environmental Analysis
USP (Ver.35) Column List
66
67
68
70
71
Semi-micro and Micro Columns
Semi-micro and Micro Columns
72
Preparative Columns
Preparative Columns ( I.D. 10mm~50mm)
Preparative Columns [Custom-made]
78
83
Information
Phase-out Products
Column Cleaning Procedures
General Precautions for Column Handling
Column Trouble Shooting
HPLC System Trouble Shooting
84
86
87
88
89
Index
Index by Product Name
90
Technical Information List
Technical Notebook, Chromato News
92
Columns for Special Separation Modes
Columns for LC/MS Analysis
Sample Pretreatment Columns
Application Fields
Column for Hydrophobic Interaction Chromatography
Columns for Affinity Chromatography
Columns for Chiral Separation
Columns for High Temperature Reversed Phase Chromatography
Column Selection
Types of Columns, Base Materials,
Functional Groups and Ligands
Separation Type
Product Name
Base Material
Functional Group,
Ligand
Page
ODP2 HP
Polyhydroxymethacrylate
—
6, 58
Asahipak
ODP-50, ODP-40
Polyvinyl alcohol
Octadecyl
8, 58
Asahipak
C8P-50
Polyvinyl alcohol
Octyl
Asahipak
C4P-50
Polyvinyl alcohol
Butyl
8
RSpak
RP18-415, DS
Styrene divinylbenzene copolymer
—
10
RSpak
DE
Polymethacrylate
—
10, 58
RSpak
DM-614
Polyhydroxymethacrylate
—
RSpak
NN
Polyhydroxymethacrylate
Sulfo
10, 58
RSpak
JJ-50
Polyvinyl alcohol
Quaternary ammonium
10, 58
Asahipak
NH2P
Polyvinyl alcohol
Amino
12, 58
ET-RP1
Polyvinyl alcohol
Octadecyl
56
ODSpak
F
Silica
Octadecyl
14
Silica
C18M, C18P
Silica
Octadecyl
14
Silicapak
E-411
Silica
—
14
Silica
5SIL
Silica
—
14
Silica
5C8
Silica
Octyl
14
Silica
5C4
Silica
Butyl
14
Silica
5CN
Silica
Cyanopropyl
14
Silica
5NPE
Silica
Nitrophenylethyl
14
Silica
5PYE
Silica
Pyrenylethyl
14
Silica
5NH
Silica
14
SUGAR, EP
SC
Styrene divinylbenzene copolymer
Aminopropyl
Sulfo (Ca2+)
SUGAR
SP0810
Styrene divinylbenzene copolymer
16
SUGAR
KS-800
Styrene divinylbenzene copolymer
Sulfo (Pb2+)
Sulfo (Na+)
RSpak
DC-613
Styrene divinylbenzene copolymer
16
SUGAR
SZ5532
Styrene divinylbenzene copolymer
Sulfo (Na+)
Sulfo (Zn2+)
USPpak
MN-431
Styrene divinylbenzene copolymer
Sulfo (Ca2+)
16
SUGAR
SH
Styrene divinylbenzene copolymer
Sulfo
20
RSpak
KC-811
Styrene divinylbenzene copolymer
Sulfo
20
IC
NI-424, I-524A
Polyhydroxymethacrylate
Quaternary ammonium
22
IC
SI
Polyvinyl alcohol
Quaternary ammonium
22
IC
YS-50
Polyvinyl alcohol
Carboxyl
24
IC
YK-421
Silica
Carboxyl
24
IC
Y-521, T-521
Styrene divinylbenzene copolymer
Sulfo
24
PROTEIN
KW-800
Silica
Hydrophilic polymer
28
KW400
Silica
Hydrophilic polymer
28
OHpak
SB-800 HQ
Polyhydroxymethacrylate
—
30
Asahipak
GS-HQ
Polyvinyl alcohol
—
32
Asahipak
GS-620 7G-P
Polyvinyl alcohol
—
32
Asahipak
GF-HQ
Polyvinyl alcohol
—
34
MSpak
GF-310
Polyvinyl alcohol
—
34, 58
GPC
KF-800, K-800,
KD-800, KF-600,
KF-400HQ, HFIP-800,
HFIP-600, LF, HT-800,
UT-800, AT-806MS
Styrene divinylbenzene copolymer
—
36, 38, 40,
42, 44, 46,
48, 58
IEC
QA-825
Polyhydroxymethacrylate
Quaternary ammonium
52
IEC
DEAE-825
Polyhydroxymethacrylate
Diethylaminoethyl
52
IEC
DEAE3N
Polyhydroxymethacrylate
Diethylaminoethyl
52
PIKESS
DEAE-2B
Polyhydroxymethacrylate
Diethylaminoethyl
52
Asahipak
ES-502N
Polyvinyl alcohol
Diethylaminoethyl
52
AXpak
WA-624
Polyhydroxymethacrylate
Diethylaminoethyl
52
IEC
SP-825
Polyhydroxymethacrylate
Sulfopropyl
54
IEC
SP-420N
Polyhydroxymethacrylate
Sulfopropyl
54
PIKESS
SP-2B
Polyhydroxymethacrylate
Sulfopropyl
54
IEC
CM-825
Polyhydroxymethacrylate
Carboxymethyl
54
Asahipak
ES-502C
Polyvinyl alcohol
54
CXpak
P-421S
Styrene divinylbenzene copolymer
Carboxymethyl
Sulfo (Na+)
Hydrophobic Interaction
HIC
PH-814
Polyhydroxymethacrylate
Phenyl
56
Affinity
AFpak
Various
Polyhydroxymethacrylate
Various ligand
56
ORpak
CDBS-453
Silica
β-Cyclodextrin derivative
56
ORpak
CRX-853
Polyhydroxymethacrylate
L-amino acid derivative
56
CLNpak
EV
Styrene divinylbenzene copolymer
—
62
CLNpak
PAE
Polyvinyl alcohol
—
62
MSpak
PK
Hydrophilic copolymers containing N-vinyl acetamide
—
64
MSpak
GF-4A
Polyvinyl alcohol
—
64
Reversed Phase &
HILIC
(Polymer-based)
Types of Columns, Base Materials, Functional Groups and Ligands
Reversed Phase &
HILIC &
Normal Phase
(Silica-based)
Ligand Exchange
Ion Exclusion
Ion Chromatography
Aqueous SEC (GFC)
Multimode
Aqueous/Organic SEC
Organic SEC (GPC)
Ion Exchange
Chiral Separation
GPC Clean-up
02
Column Switching
Pretreatment
8
10
16
16
16
54
Liquid chromatography (LC) uses liquid as mobile phase (eluent). It is an analytical method that separates a mixture of compounds
based on their physical and chemical differences. High performance liquid chromatography (HPLC) is a method that introduces
the mobile phase under high-pressure conditions that results in rapid and high-performance separations. The various interactions
between the analyte, stationary phase (packing material), and mobile phase are the key factors for the separation. A wide variety
of separation mechanism can be obtained by using particular combinations of stationary and mobile phases.
Characteristics
Separation mode
• Separation is based on hydrophilic interaction.
• A high polarity stationary phase is used.
• Typically the mobile phase contains a mixture of organic solvents such as acetonitrile and aqueous solvents (water or buffer).
• Using the higher polarity mobile phase causes a faster elution.
• Applicable for the analysis of high polar substances.
Normal Phase
Chromatography
(NPC)
• Separation is based on the partition equilibrium between the stationary phase and the mobile phase.
• The polarity of the stationary phase is higher than that of the mobile phase.
• Typically the mobile phase contains a mixture of organic solvents with different polarities such as hexane and isopropanol.
• Using the higher polarity mobile phase causes a faster elution.
Ligand Exchange
Chromatography
(LEX)
• Separation is based on differences in analytes’ coordination complex.
• Stationary phase modified with metal sulfonate complex ion.
• Works in combination with size exclusion or HILIC modes.
Ion Exclusion
Chromatography
(IEX)
• Separation is based on electrostatic interaction (repulsion) between the ion exchanger and ionic solutes.
• Dissociated ionic molecules elute faster than non-dissociated forms.
• Used mainly for the analysis of organic acids.
Ion
Chromatography
(IC)
• Separation is based on electrostatic interaction (bonding) between the ion exchanger and ionic solutes.
• Has a relatively small ion exchange capacity.
• Electrical conductivity detector can be used with low-salt concentration mobile phase.
• Used mainly for the analysis of inorganic compounds.
Size Exclusion
Chromatography
(SEC)
• Network or pores on the surface of the packing material work as molecular sieve to separate molecules based on their sizes.
• To separate molecules solely based on their sizes, it requires an analytical condition without any analyte and packing gel
interaction.
• The bigger the molecule size, the faster the elution sequence.
• Used for molecular weight or molecular distribution determination of macromolecules and qualification of oligomers.
Ion Exchange
Chromatography
(IEC)
• Separation is based on electrostatic interactions between the ion exchanger and ionic solutes.
• The mobile phase of choice should have a sufficient buffering capacity at the pH that produces the largest charge
differences between the analyte of interest.
• The elution position is optimized by varying the pH, salt concentration, and/or ionic strength of the mobile phase.
Hydrophobic
Interaction
Chromatography
(HIC)
• Separation is based on hydrophobic interaction.
• Hydrophobic functional group is modified on the stationary phase.
• Adsorption of analytes generally occurs at a high salt concentration and they are released by lowering the salt concentration.
• Used mainly for the analysis of proteins.
Affinity
Chromatography
(AFC)
• Separation is based on adsorption of the analyte to the specific biologically derived ligand pair.
• Highly selective.
• A buffer solution with the appropriate pH and ionic strength is selected based on the type of ligand, analytes, and their interaction.
• Used mainly for the purification and concentration of biological active substances.
Chiral Separation
Chromatography
(CS)
• Separation of optical isomers using chiral selectors.
• Highly selective.
Multimode
Chromatography
• Separation is based on the combination of different modes.
Column Selection by Sample Character and
Separation Mode
Sample
MW
Separation
Mode
Sample
Solubility
Sample
MW
Separation
Mode
≥ 2,000
SEC
Column Selection by Sample Character and Separation Mode
Hydrophilic
Interaction
Chromatography
(HILIC)
HPLC Separation Modes
Reversed Phase
Chromatography
(RPC)
• Separation is based on the partition equilibrium between stationary phase and mobile phase.
• The polarity of the stationary phase is lower than that of the mobile phase.
• Typically the mobile phase contains a mixture of organic solvents (methanol, acetonitrile, or THF) and aqueous solvents
(water or buffer).
• Using the lower polarity mobile phase causes a faster elution.
Sample
Solubility
Column Selection
HPLC Separation Modes
RPC
LEX
IEX
≥ 2,000
SEC
IEC
HIC
AFC
Aqueous
soluble
RPC
Organic
soluble
HILIC
RPC
LEX
IEX
≤ 2,000
IC
≤ 2,000
NPC
SEC
IEC
AFC
CS
SEC
RPC : Reversed Phase Chromatography
HILIC : Hydrophilic Interaction Chromatography
NPC : Normal Phase Chromatography
LEX : Ligand Exchange Chromatography
IEX : Ion Exclusion Chromatography
: Ion Chromatography
IC
SEC : Size Exclusion Chromatography
IEC : Ion Exchange Chromatography
HIC : Hydrophobic Interaction Chromatography
AFC : Affinity Chromatography
: Chiral Separation Chromatography
CS
03
Column Selection
Column Selection (Application)
Pharmaceuticals, Cosmetics
Separation Mode
Hydrophobic
substances
Pharmaceuticals
Column Selection (Application) • Pharmaceuticals, Cosmetics
Metabolites
RPC
Food
Page
6, 8, 10, 14,
56, 58
HILIC
12, 58
IEC+RPC
10, 58
LEX+SEC
16
Substances in
bio-fluid
RPC
6, 58
(serum·plasma·urine)
SEC+RPC 32,34,58,64
Hydrophilic
substances
Polyalcohols
Separation Mode
RPC
10, 58
LEX+SEC
16
Monosaccharides
Disaccharides
Sugar alcohols
Oligosaccharides
Organic acids
LEX+HILIC 16
Moisturizers
SEC
Protein
hydrolysates
RPC
30, 34, 58
8, 10, 58
SEC
28
Mucopolysaccharides SEC
30
Nutritional
ingredients
Water-soluble
vitamins
SEC+RPC 34, 58
Emulsifiers
Preservatives
HILIC
12, 58
LEX+SEC
16
LEX+HILIC
16
HILIC
12, 58
LEX+HILIC
16
SEC
16, 30, 32
Low molecular
water-soluble dietary fiber SEC
Polysaccharides
Page
32
SEC
16, 30
RPC
6, 10, 58
IEX+RPC
10, 20, 58
IC
22
RPC
6, 8, 10, 58
IEC+RPC
10, 58
HILIC
12, 58
RPC
8, 58
Surfactants
Paraben
Dehydroacetic acid
SEC
36, 42, 58
RPC
8, 10, 14, 58
Fat-soluble vitamins NPC
14
SEC
36, 42, 58
RPC
10, 14, 58
SEC
34,36,38,42
Fatty acids
Optical active
materials
CS
56
Nucleic acids (umami) IEC+SEC
32
IEC+IEX+RPC 10, 58
• Food
Amino acids
IC
24
IEC
54
RPC
8, 10, 56, 58
HILIC
12, 58
RPC
10, 58
IEC+RPC
10, 58
IC
22
Mycotoxin
RPC
14
Pretreatment of
residual pesticides
( GPC
Clean-up )
• New Materials
Food additives
Food safety
Separation Mode
Pesticides
SEC
62
RPC : Reversed Phase Chromatography
HILIC : Hydrophilic Interaction Chromatography
New Materials
NPC : Normal Phase Chromatography
LEX : Ligand Exchange Chromatography
IEX
: Ion Exclusion Chromatography
IC
: Ion Chromatography
SEC : Size Exclusion Chromatography
IEC
: Ion Exchange Chromatography
HIC
: Hydrophobic Interaction Chromatography
Synthetic
polymers
04
: Chiral Separation Chromatography
30,34,40,42,44
Polar organic solvent soluble
High temperature/
Ultra high temperature
SEC
46
Water-soluble
28,30,32,34
RPC
Additives
Oligomers
Organic solvent soluble
Polar organic solvent soluble
Water-soluble
Page
34,36,38,42,44
Organic solvent soluble
AFC : Affinity Chromatography
CS
Separation
Mode
8, 10, 14
34,36,38,42
SEC
30,34,40,42
28,30,32,34
Environment
Separation Mode
Nucleobases
Nucleotides
Nucleosides
Genomics
RPC
10, 58
IEC+SEC
10, 32, 58
Separation Mode
Anions
IC
22
IC
22
IEC
58
Oxyhalides
IEC
52
RPC
10, 58
Cyanide
Cyanogen chloride
IEX
20
IEC+SEC
32
Cations
IC
24
IEC
52
RPC
8, 14
SEC+RPC
34, 58
IC
22
Water quality
DNA, RNA
Page
SEC
30, 34
RPC
8, 58
Surfactants
Perchloric acids
10, 58
IEC
58
IEC
24, 54
RPC
10, 58
IEC+SEC
32
IEC+RPC
10, 58
RPC
8, 10, 58
IC
22
SEC
28,30,32,34
Anions
IC
22
IEC
52, 54
Heavy metals
IC
24
HIC
56
Humic substances
SEC
30
RPC
8, 10, 58
Organic arsenic
IEX+RPC
10, 58
SEC
28,30,32,34
RPC
10
IEC
52, 54
IEC+RPC
10, 58
HIC
56
IC
22
AFC
56
Pesticides
Proteomics
Peptides
Proteins
Glycoproteins
Glycomics
HILIC
12, 58
AFC
56
HILIC
12, 58
LEX+SEC
16
LEX+HILIC
16
Sugar chains
Monosaccharides
Sialic acids
Uronic acids
Aldonic acids
IEX+SEC
20
RPC
6, 8, 10, 58
IEC
54
RPC
8, 58
HILIC
12, 58
SEC
30, 34, 58
NPC
14
SEC
34,36,42,58
SEC
30
AFC
56
Soil
Pesticides
Environmental
hormones
Steroids
Phospholipids
Lipid
SEC
GPC
Clean-up
62
PCBs
Benzo[a]pyrene
(
Monosaccharides
Oligosaccharides
HILIC
12, 58
LEX+SEC
16
Oligosaccharides
Alcohols
Furfural
LEX+SEC
16
Saccharides
Organic acids
Alcohols
Furfural
IEX+RPC+SEC
20
Hemicelluloses
Celluloses
SEC
40, 44
Cations
IC
24
Fatty acid glycerides
SEC
34, 58
)
Bioethanols
Amines
Hormone
Pretreatment of
Phthalates
• Environment
IEC+IEX+RPC
Amino acids
Column Selection (Application) • Biotechnology
Oligo nucleic
acids
Page
Column Selection
Biotechnology
Biodiesels
Fatty acid methyl esters RPC
10
Organic acids
22
IC
Lipoproteins
05
Standard Analysis
Columns
Columns for Polymer-based
Reversed Phase Chromatography
Features
ODP2 HP
Provides nearly twice as large theoretical plate number compared with generally available
polymer-based reversed phase columns
Column offers enhanced retention of high polar substances compared with ODS column
Suitable for reversed phase analysis of small molecules such as pharmaceutical compounds in the
presence of protein matrix
Columns for Polymer-based Reversed Phase Chromatography
Ideal for LC/MS analysis of high polar compounds
Note
book
No.6
News
No.30, 36
See also page 8, "Features and applications of different packing materials used for columns for reversed phase, hydrophilic interaction and
normal phase chromatography".
Standard columns
Product Code
Plate Number
(TP/column)
Product Name
Functional Particle Size
(µm)
Group
Pore Size
(Å)
Column Size
(mm)
I.D. x Length
Shipping Solvent
F7622001
ODP2 HP-4B
≥ 3,500
−
5
40
4.6 × 50
H2O/CH3CN=55/45
F7622002
ODP2 HP-4D
≥ 13,000
−
5
40
4.6 × 150
H2O/CH3CN=55/45
F7622003
ODP2 HP-4E
≥ 17,000
−
5
40
4.6 × 250
H2O/CH3CN=55/45
F6714010
ODP2 HPG-4A
(guard column)
−
5
−
4.6 × 10
H2O/CH3CN=55/45
F7622004
ODP2 HP-2B
≥ 3,000
−
5
40
2.0 × 50
H2O/CH3CN=55/45
F7622005
ODP2 HP-2D
≥ 7,000
−
5
40
2.0 × 150
H2O/CH3CN=55/45
F6714011
ODP2 HPG-2A
(guard column)
−
5
−
2.0 × 10
H2O/CH3CN=55/45
Base Material : Polyhydroxymethacrylate
Tolerance of ODP2 HP for alkaline condition
Comparison of chromatograms obtained before
and after passage of alkaline eluent
ODS
1
Relative theoretical plate number for pyridine
ODP2 HP-4D
1
2
2
Before passage
Before passage
After 500 hr
passage
0
After 24 hr
passage
5
10
min
0
Correlation between alkaline eluent
passage time and relative theoretical
plate number
Sample : 5μL
1. Pyridine 200μg/mL
2. Phenol
430μg/mL
5
10
min
(%)
120
100
80
60
40
ODP2 HP-4D
ODS
20
0
0
200
400
Alkaline eluent passage time (hr)
Condition
Eluent passage conditions for alkali tolerance test
Column
Eluent
: 10mM Sodium phosphate buffer(pH12)/CH3CN=45/55
Flow rate
: 0.6mL/min
Column temp. : 30˚C
: Shodex ODP2 HP-4D
ODS from other manufacturer
Eluent
: H2O/CH3OH=70/30
Flow rate
: 1.0mL/min
Detector
: UV(254nm)
Column temp. : 40˚C
06
*Contact Shodex or our distributors near you for customized columns.
600
Sample : 0.1% each, 10μL
1. Imidazole
2. 2-Methylimidazole
3. 4-Methylimidazole
Sample : 5μL
1. Phenol
300mg/L
2. Methyl benzoate 350mg/L
3. Toluene
1000mg/L
4. Naphthalene
150mg/L
ODP2 HP-4D
1
Standard Analysis
Columns
Imidazoles
Comparison between ODP2 HP and ODP-50 (existing)
ODP- 50 4D (existing)
1
2
3
2
3
TP: 11,400
3
1
4
2
TP:5,700
4
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10 min
0
Shodex ODP2 HP-4D
H2O/CH3CN=55/45
0.6mL/min
UV(254nm)
40˚C
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10 min
0
Shodex Asahipak ODP-50 4D
H2O/CH3CN=35/65
0.6mL/min
UV(254nm)
40˚C
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10 min
Shodex ODP2 HP-4E
10mM Na2HPO4 aq./CH3CN=90/10
0.8mL/min
UV(220nm)
40˚C
Influence of repeated protein injection on column pressure
160
ODP2 HP series column is packed with gels having increased
surface polarity and small pore size. This enables to remove and
prevent adsorption of proteins. BSA was injected multiple times
to an ODS and an ODP2 HP columns. A significant column
pressure increase was observed with the ODS column, while no
considerable change was observed with the ODP2 HP column
even after 140 injections.
150
Ratio of Pressure (%)
140
130
Sample : 7.0mg/mL BSA, 5μL
ODS
120
110
ODP2 HP-2B
Column
100
90
20
0
40
60
Columns for Polymer-based Reversed Phase Chromatography
0
80
100
120
140
: Shodex ODP2 HP-2B
ODS from other manufacturer
Eluent
: 1mM Ammonium acetate aq./CH3CN=90/10
Flow rate
: 0.2mL/min
Detector
: UV(220nm)
Column temp. : 30˚C
Number of Injection
Taking original pressure as 100%
Simultaneous analysis of multi symptom cold formula in serum
2
Sample : 10μL 1. Dihydrocodeine phosphate
2. Acetaminophen
3. Aspirin
4. Salicylamide
5. Salicylic acid
6. Bromhexine hydrochloride
7. Ibuprofen
Standards
2
5
Blood serum
(without pretreatment)
Sample : Human serum, 10μL
1. Dihydrocodeine phosphate
2. Acetaminophen
3. Aspirin
4. Salicylamide
5. Salicylic acid
3
1
3
4
1
6
7
4
0
10
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
20
30
Shodex ODP2 HP-4E
0.1% TFA aq./CH3CN=70/30
0.4mL/min
UV(235nm)
40˚C
40
50
60 min
0
10
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
20
5
30
40
50 min
Shodex ODP2 HP-4E
0.1% TFA aq./CH3CN=70/30
0.4mL/min
UV(235nm)
40˚C
Data courtesy of Katsuko Hara.MT Yutaka Komiyama.Ph.D., Department of Clinical Sciences and Laboratory Medicine, Kansai Medical University.
07
Standard Analysis
Columns
Columns for Polymer-based
Reversed Phase Chromatography
Features
ODP-50, C8P-50, C4P-50
Relative large pore size is suitable for the analysis of amino acids, peptides, and proteins
Usable in a wide pH range from acidic to alkaline (pH 2 to 13)
Usable in 100% water or buffer solution
Best used for the analysis of basic substances
Columns for Polymer-based Reversed Phase Chromatography
News
ODP-40
No.35
Preparative
Columns
p.78
Semi-micro
Micro Columns
p.72
Higher performance type of ODP-50 series
News
No.1
Standard columns
Product Code
Product Name
Plate Number
(TP/column)
Functional
Group
Particle Size Pore Size
(µm)
(Å)
Column Size
(mm)
I.D. x Length
Shipping Solvent
F7621001
Asahipak ODP-40 4D
≥ 11,000
Octadecyl
4
250
4.6 × 150
H2O/CH3CN=35/65
F7621002
Asahipak ODP-40 4E
≥ 17,000
Octadecyl
4
250
4.6 × 250
H2O/CH3CN=35/65
F7620002
Asahipak ODP-50 6D
≥ 9,000
Octadecyl
5
250
6.0 × 150
H2O/CH3CN=35/65
F7620001
Asahipak ODP-50 6E
≥ 14,000
Octadecyl
5
250
6.0 × 250
H2O/CH3CN=35/65
F6710001
Asahipak ODP-50G 6A
(guard column)
Octadecyl
5
−
6.0 × 10
H2O/CH3CN=35/65
F6710023
Asahipak ODP-50 4B
≥ 2,500
Octadecyl
5
250
4.6 × 50
H2O/CH3CN=35/65
F7620004
Asahipak ODP-50 4D
≥ 9,000
Octadecyl
5
250
4.6 × 150
H2O/CH3CN=35/65
F7620003
Asahipak ODP-50 4E
≥ 14,000
Octadecyl
5
250
4.6 × 250
H2O/CH3CN=35/65
F6710022
Asahipak ODP-50G 4A
(guard column)
Octadecyl
5
−
4.6 × 10
H2O/CH3CN=35/65
F7620009
Asahipak ODP-50 2D
≥ 5,000
Octadecyl
5
250
2.0 × 150
H2O/CH3CN=35/65
F6713001
Asahipak ODP-50G 2A
(guard column)
Octadecyl
5
−
2.0 × 10
H2O/CH3CN=35/65
F7620006
Asahipak C8P-50 4D
≥ 7,000
Octyl
5
250
4.6 × 150
H2O/CH3CN=35/65
F7620005
Asahipak C8P-50 4E
≥ 11,000
Octyl
5
250
4.6 × 250
H2O/CH3CN=35/65
F6710002
Asahipak C8P-50G 4A
(guard column)
Octyl
5
−
4.6 × 10
H2O/CH3CN=35/65
F7620008
Asahipak C4P-50 4D
≥ 6,000
Butyl
5
250
4.6 × 150
H2O/CH3CN=35/65
F7620007
Asahipak C4P-50 4E
≥ 9,000
Butyl
5
250
4.6 × 250
H2O/CH3CN=35/65
F6710003
Asahipak C4P-50G 4A
(guard column)
Butyl
5
−
4.6 × 10
H2O/CH3CN=35/65
Base Material : Polyvinyl alcohol
Features and applications of different packing materials used for columns for reversed phase,
hydrophilic interaction and normal phase chromatography
Large
Proteins
Pore size of packing materials
Saccharides,
Sugar alcohols,
Hydrophilic low molecular
weight substances
Polycyclic
aromatic
compounds
Peptides
RP18-415
C4P-50
C8P-50
5C4
5C8
ODP-40,50
DS-413,613
DM-614
Silicapak
SIL
5NH
5CN
ODS
NH2P-40,50
ODP2 HP
DE
Vitamins
Low molecular weight organic compounds,
drugs, organic acids, food additives, etc.
Small
08
High
Polarity of packing materials
*Contact Shodex or our distributors near you for customized columns.
Low
1
C8
Sample :
1. Acetophenone
2. Butyrophenone
3. Hexanophenone
4. Heptanophenone
5. Octanophenone
Sample : Ethyleneglycol, n-Alkylalcohol
C4P-50
C6
C10
C4
C12
EG
Elution volume (mL)
C8
C6
C4
EG
ODP-50
15
C14
2
10
C8P-50
3
4
5
5
C8P-50
C4P-50
C10 C12
330hr
2
4
6
8
10 12 14
230hr
Carbon number (n)
Relationship between the carbon number of
alkylalcohols and elution volume
C8
0hr
Column
ODP-50
C10
C12
C14
0
90hr
5
10
15
20
: Shodex Asahipak ODP-50 4D
Asahipak C8P-50 4D
Asahipak C4P-50 4D
Eluent
: H2O/CH3OH=20/80
Flow rate
: 0.6mL/min
Detector
: RI
Column temp. : 30˚C
0
10
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
20 min
Shodex Asahipak ODP-50 4D
10mM NaOH aq.(pH12.0)/CH3CN=35/65
0.6mL/min
UV(254nm)
30˚C
25 min
Nonionic surfactants
Local anesthetics
Dissociation of tertiary amino groups in basic drugs can be suppressed by making pH of the eluent
higher than pKa of the amino groups. This increases the relative hydrophobicity of the basic drugs,
thereby allowing the column to retain the drugs stronger and provide baseline separation of them.
Sample : 20μL
1. Heptaoxyethylenedodecylether(PAR-Co) 1μg/L
2. PAR
Sample :
3
pH3
pH7
1. Benzocaine
pH11
H2N
2
COOC2H5
1
1
1
2
2. Lidocaine
CH3
3
NHCOCH2N
2
1
C2H5
C2H5
3
CH3
2
3. Tetracaine
COOCH2CH2N
CH3
CH3
0
1
2
3
4
5
6
Columns for Polymer-based Reversed Phase Chromatography
C14
C4 C6
EG
Standard Analysis
Columns
Tolerance of ODP-50 for alkaline condition
Alkylalcohols
7
min
NH(CH2)3CH3
0
0
20 min
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
20 min
0
20 min
Column
Eluent
: Shodex Asahipak ODP-40 4D
: 10mM Sodium tetraborate aq.
/CH3OH=62/38
Flow rate
: 0.8mL/min
Detector
: VIS(510nm)
Column temp. : 40˚C
Shodex AsahipakODP-50 4D
25mM Phosphate buffer/CH3CN=60/40
0.6mL/min
UV(254nm)
30˚C
Fat-soluble vitamins
Sample : 20μL
1. Vitamin K3
2. Vitamin A
3. Vitamin A acetate
4. Vitamin D2
5. Vitamin D3
6. Vitamin E acetate
7. Vitamin E
8. Vitamin K1
Macrolide antibiotics
Gradient analysis of proteins and peptides
Sample : 0.1% each, 10μL
1. Erythromycin
2. Azithromycin
1.5μg/mL
1.0 IU/mL
0.5 IU/mL
13.2μg/mL
13.2 IU/mL
2.4μg/mL
2.5μg/mL
2.4μg/mL
9
50%CH3CN
6
2
4
3
7
1
1
2
1
2
5
Sample
Mw
Recovery
(%)
1
Lys-Bradykinin
1188
97
2
Bradykinin
1060
92
3
Met-Enkephalin
574
97
4
Neurotensin
5
Leu-Enkephalin
6
Substance P
7
Bacitracin
1450
81
8
Insulin
5750
95
9
Insulin B chain
10
8
20%CH3CN
4
11
12
10 Lysozyme
6 7
3
Sample : 40μL
8
5
11 Mastoparan
12 Myoglobin
0
5
10
15
20
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
15
20
25
min
Shodex Asahipak ODP-50 4E
CH3CN/CH3OH=50/50
0.6mL/min
UV(280nm)
30˚C
Column
Eluent
99
556
100
1348
93
3496
91
14300
96
1479
96
17500
83
25
min
0
0
1673
: Shodex Asahipak ODP-40 4E
: 40mM Potassium phosphate
buffer(pH11.0)/CH3CN=40/60
Flow rate
: 0.5mL/min
Detector
: UV(223nm)
Column temp. : 40˚C
10
20 min
Column
Eluent
: Shodex Asahipak ODP-50 6D
: (A); 0.05% TFA aq./CH3CN=80/20
(B); 0.05% TFA aq./CH3CN=50/50
Linear gradient; (A) to (B), 20min
Flow rate
: 1.0mL/min
Detector
: UV(220nm)
Column temp. : 30˚C
09
Standard Analysis
Columns
Columns for Polymer-based
Reversed Phase Chromatography
Features
Columns for Polymer-based Reversed Phase Chromatography
RP18-415
Large pore size is suitable for the analysis of proteins and peptides
DS-613, 413
Suitable for reversed phase analysis of highly hydrophilic substances, which
cannot be retained well by ODS columns
DE
Polymer-based columns, with similar polarity to that of ODS columns, can be
used in general and wide purpose
Semi-micro
Micro Columns
p.72
Preparative
Columns
p.78
Preparative
Columns
p.78
Semi-micro
Micro Columns
p.72
Wide working pH range (2-12), usable in 100% water and buffer solutions
News
No.14, 23, 24, 35
DM-614
Suitable for the analysis of amino acids and water-soluble vitamins
NN
The packing material contains sulfo groups, and supports multimode (reversed
phase and cation exchange) analysis
Ideal for analysis of complex samples containing neutral and ionic substances
Note
book
JJ-50
No.3
News
No.7
The packing material contains trace amounts of quaternary ammonium groups, and
supports multimode (reversed phase and anion exchange) analysis
Ideal for analysis of complex samples containing neutral and ionic substances
News
No.7
See also page 8, "Features and applications of different packing materials used for columns for reversed phase, hydrophilic interaction and
normal phase chromatography".
Standard columns
Product Code
Product Name
F7009000
RSpak RP18-415
F6709558
Plate Number Functional
Group
(TP/column)
Base Material
Particle Pore Column Size
Size
Size
(mm)
(Å) I.D. x Length
(µm)
Shipping Solvent
≥ 5,000
−
Styrene divinylbenzene copolymer
6
450
4.6 × 150
H2O/CH3CN=5/95
RSpak RP18-G
(guard column)
−
Styrene divinylbenzene copolymer
6
−
4.6 × 10
H2O/CH3CN/THF=40/30/30
F7001001
RSpak DS-613
≥ 6,500
−
Styrene divinylbenzene copolymer
6
200
6.0 × 150
H2O/CH3CN/THF=30/40/30
F7001012
RSpak DS-413
≥ 11,000
−
Styrene divinylbenzene copolymer
3.5
200
4.6 × 150
H2O/CH3CN/THF=40/30/30
F6700140
RSpak DS-G
(guard column)
−
Styrene divinylbenzene copolymer
10
−
4.6 × 10
H2O/CH3CN/THF=30/40/30
F7001004
RSpak DE-613
≥ 7,000
−
Polymethacrylate
6
25
6.0 × 150
H2O
F7001005
RSpak DE-413
≥ 11,000
−
Polymethacrylate
4
25
4.6 × 150
H2O/CH3CN=50/50
F7009030
RSpak DE-413L
≥ 17,000
−
Polymethacrylate
4
25
4.6 × 250
H2O/CH3CN=50/50
F6700150
RSpak DE-G
(guard column)
−
Polymethacrylate
10
−
4.6 × 10
H2O
F7001007
RSpak DE-213
≥ 8,000
−
Polymethacrylate
4
25
2.0 × 150
H2O/CH3CN=50/50
F6700151
RSpak DE-SG
(guard column)
−
Polymethacrylate
6
−
2.0 × 10
H2O/CH3CN=50/50
F7001002
RSpak DM-614
≥ 4,500
−
Polyhydroxymethacrylate
10
200
6.0 × 150
5mM H3PO4 aq.
F6700160
RSpak DM-G
(guard column)
−
Polyhydroxymethacrylate
12
−
4.6 × 10
5mM H3PO4 aq.
F7008140
RSpak NN-814
≥ 9,000
Sulfo
Polyhydroxymethacrylate
10
200
8.0 × 250
0.1M Sodium phosphate buffer
(pH3.0)
F7008150
RSpak NN-614
≥ 4,000
Sulfo
Polyhydroxymethacrylate
10
200
6.0 × 150
0.1M Sodium phosphate buffer
(pH3.0)
F6700510
RSpak NN-G
(guard column)
Sulfo
Polyhydroxymethacrylate
10
−
6.0 × 50
0.1M Sodium phosphate buffer
(pH3.0)
F7008160
RSpak NN-414
≥ 6,000
Sulfo
Polyhydroxymethacrylate
10
200
4.6 × 150
0.1M Sodium phosphate buffer
(pH3.0)
F7008240
RSpak JJ-50 4D
≥ 4,500
Polyvinyl alcohol
5
100
4.6 × 150
H2O/CH3CN=40/60
F7008220
RSpak JJ-50 2D
≥ 3,500
Quaternary
ammonium
Quaternary
ammonium
Polyvinyl alcohol
5
100
2.0 × 150
H2O/CH3CN=40/60
10
*Contact Shodex or our distributors near you for customized columns.
Alkylbenzenes
Fatty acid methyl esters
Sample : 5μL
1. m-Cresol
2. 2,4-Xylenol
3. Benzene
4. Toluene
5. Ethylbenzene
6. n-Propylbenzene
Recovery (%)
1. Ribonuclease A
3
2. Insulin
1
6
100
4. Lysozyme
100
2
4
2
1
98
3. Cytochrome c
5. BSA
5
93
98
6. Myoglobin
108
7. Ovalbumin
—
Sample : 0.2% each, 20μL
1. Methyl linoleate
2. Methyl palmitate
3. Methyl oleate
4. Methyl stearate
0.1%
0.1%
0.5%
0.5%
0.5%
0.5%
4
1
5
3
7
Standard Analysis
Columns
Separation and recovery rate of standard
proteins
2
6
3
4
10
20
30 min
0
: Shodex RSpak RP18-415
: (A); 0.1% TFA aq./CH3CN=99/1
(B); 0.1% TFA aq./CH3CN=5/95
Linear gradient; 20%(B) to 60%(B), 20min
Flow rate
: 1.0mL/min
Detector
: UV(220nm)
Column temp. : Room temp.
2
4
6
8
10 min
0
Column
Eluent
Organic acids
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Shodex RSpak DS-613
H2O/CH3CN/THF=30/40/30
1.0mL/min
UV(254nm)
40˚C
Column
:
Eluent
:
Flow rate
:
Detetor
:
Column temp. :
Food additives (Preservatives)
Sample : 5μL
1. Glyoxylic acid
2. Tartaric acid
3. Malic acid
4. Lactic acid
5. Malonic acid
6. Acetic acid
7. Succinic acid
8. Levulinic acid
9. Propionic acid
2
1
3
1.78mg/mL
1.95mg/mL
2.06mg/mL
2μL/mL
1.95mg/mL
2μL/mL
2.05mg/mL
1.95mg/mL
2μL/mL
Sample : 10μL
1. Saccharin sodium
2. p-Hydroxybenzoic acid
3. Sorbic acid
4. Benzoic acid
5. Methyl paraben
6. Dehydroacetic acid
7. Ethyl paraben
8. Propyl paraben
1
5
10 min
Shodex RSpak DS-413
H2O/CH3CN/THF=25/45/30
1.0mL/min
RI
40˚C
Diols
Sample : 1% each, 7.5μL
HO(CH2)nOH
n=2
0.005%
0.005%
0.02%
0.02%
0.01%
0.01%
0.02%
0.02%
3
4
7
5
5
4
6
2
7
5
3
4
8
6
8
9
0
1
2
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
3
4
5
6
7
6
8 min
0
5
10
15
20 min
0
Column
Eluent
: Shodex RSpak DE-413
: 50mM KH2PO4 + 0.1% H3PO4 aq.
/CH3CN=65/35
Flow rate
: 1.0mL/min
Detector
: UV(210nm)
Column temp. : 40˚C
Shodex RSpak DE-413
10mM H3PO4 aq.
1.0mL/min
RI
50˚C
Amino acids
Carnitine
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
Columns for Polymer-based Reversed Phase Chromatography
0
15 min
Shodex RSpak DE-613
H2O
1.0mL/min
RI
60˚C
Phenylenediamine isomers
Sample : 0.1% each, 20μL
1. Aspartic acid
2. Glycine
3. Alanine
4. Valine
5. Methionine
6. Isoleucine
Sample : 20μL
1. L-Carnitine 0.1%
1
Sample : 100mg/L each, 20μL
1. p-Phenylenediamine
2. m-Phenylenediamine
3. o-Phenylenediamine
1
2
1
OH O
2
3
N+
3
4
OH
5
0
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
15
20
Shodex RSpak NN-814
0.1M H3PO4 aq.
1.0mL/min
UV(210nm)
25˚C
25
min
15
20
25
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
30
35
40
6
45
Shodex RSpak NN-814
40mM H3PO4 aq.
1.0mL/min
RI
40˚C
50
55
min
0
5
10
15 min
Column
Eluent
: Shodex RSpak JJ-50 4D
: 25mM Ammonium acetate buffer
(pH9.2)/CH3CN=70/30
Flow rate
: 0.4mL/min
Detector
: UV(254nm)
Column temp. : 30˚C
11
Standard Analysis
Columns
Columns for Polymer-based
Hydrophilic Interaction Chromatography (HILIC)
Features
Suitable for saccharides analysis by hydrophilic interaction chromatography (HILIC)
Polymer-based packing material provides excellent chemical stability and minimum
deterioration over extended time period
Washable with alkaline solution
Also suitable for evaporative light scattering detector, corona charged aerosol detector,
and LC/MS
NH2P-50
Columns for Polymer-based Hydrophilic Interaction Chromatography (HILIC)
Note
book
No.2
News
No.6, 14, 17, 32
Preparative
Columns
Provides higher theoretical plate number compared to NH2P-50 series
Achieves 2-3 times improved detection sensitivity even with a conventional HPLC system
Uses less than 50% solvent compared to NH2P-50 4E
NH2P-40 3E
Semi-micro
Micro Columns
No.44
News
p.72
See also page 8, "Features and applications of different packing materials used for columns for reversed phase, hydrophilic interaction and
normal phase chromatography".
Standard columns
Product Code
Plate Number
(TP/column)
Product Name
Functional Particle Size
Group
(µm)
Pore Size
(Å)
Column Size
(mm)
I.D. x Length
Shipping Solvent
F7630005
Asahipak NH2P-50 4B
≥ 1,500
Amino
5
100
4.6 × 50
H2O/CH3CN=25/75
F7630002
Asahipak NH2P-50 4D
≥ 5,500
Amino
5
100
4.6 × 150
H2O/CH3CN=25/75
F7630001
Asahipak NH2P-50 4E
≥ 7,500
Amino
5
100
4.6 × 250
H2O/CH3CN=25/75
F6710016
Asahipak NH2P-50G 4A
(guard column)
Amino
5
−
4.6 × 10
H2O/CH3CN=25/75
F7630006
Asahipak NH2P-50 2D
≥ 3,500
Amino
5
100
2.0 × 150
H2O/CH3CN=25/75
F6713000
Asahipak NH2P-50G 2A
(guard column)
Amino
5
−
2.0 × 10
H2O/CH3CN=25/75
F7630007
Asahipak NH2P-40 3E
≥ 8,500
Amino
4
100
3.0 × 250
H2O/CH3CN=25/75
F6710030
Asahipak NH2P-50G 3A
(guard column)
Amino
5
−
3.0 × 10
H2O/CH3CN=25/75
F6710100
Asahipak NH2P-LF
(line filter)
Amino
−
−
8.0 × 75
H2O/CH3CN=25/75
Base Material : Polyvinyl alcohol
Comparison of polymer-based (NH2P-50) and silica-based amino columns
Silica-based amino column
NH2P-50 4E
First use
After 180hr
1
After 120hr
1
3
2
2
4
0
First use
Polymer-based amino column, NH2P-50,
showed highly repeatable result (both
retention time and peak shapes) for all
saccharides tested; proving its high
tolerance to time degradation.
1
3
10
3
1. Fructose
2. Glucose
3. Sucrose
4. Maltose
20 min
2
4
4
0
10
Ve (mL)
20
20 min
10
0
1
1. Fructose
2. Glucose
3. Sucrose
4. Maltose
20 min
2 3
4
0
10
20 min
Ve (mL)
20
18
18
Maltose
Maltose
16
16
Sucrose
14
Column
14
Sucrose
12
12
10
Glucose
10
Glucose
8
Fructose
8
Fructose
50
12
p.79
100 150 200
Time (hr)
50
100 150 200
Time (hr)
*Contact Shodex or our distributors near you for customized columns.
: Shodex Asahipak NH2P-50 4E
Silica-based amino column
from other manufacturer
Eluent
: H2O/CH3CN=25/75
Flow rate
: 1.0mL/min
Detector
: RI
Column temp. : 30˚C
Chitooligosaccharides
Sample :
Fructooligosaccharide syrup, 2.5%, 20μL
1. Fructose
2. Glucose
3. Sucrose
4. 1-Kestose
5. Nystose
6. 1-Fructofuranosyl-D-nystose
Oligogalacturonic acids
Sample : Chitooligosaccharides 2%, 20μL
1. D-Glucosamine
2. Chitobiose hydrochloride
3. Chitotriose hydrochloride
4. Chitotetraose hydrochloride
5. Chitopentaose hydrochloride
6. Chitohexaose hydrochloride
2
Sample : Oligogalacturonic acids
1. Galacturonic acid
2. Oligogalacturonic acid dimer
3. Oligogalacturonic acid trimer
4. Oligogalacturonic acid tetramer
5. Oligogalacturonic acid pentamer
6. Oligogalacturonic acid hexamer
4
3
1
Standard Analysis
Columns
Fructooligosaccharide syrup
2
6
3
4
4
5
5
5
2
6
1
0
6
5
10
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
15
1
20
25
min
0
5
Shodex Asahipak NH2P-50 4E
H2O/CH3CN=30/70
1.0mL/min
RI
25˚C
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
1
2
Silica-based amide column
(4.6mmI.D. x 150nm)
1
2
5
0
10
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Shodex Asahipak NH2P-50 4E
H2O/CH3CN=30/70
1.0mL/min
RI
25˚C
Saccharide analysis using corona charged aerosol detector
Sample : 40μg/mL each, 5μL
1. Fructose
2. Glucose
15 min
15
Shodex Asahipak NH2P-50 4E
0.3M Sodium phosphate buffer(pH4.4)
1.0mL/min
UV(210nm)
40˚C
Sample offered by Prof. Yoshino in Kyoto Women's Univ.
Pyridylaminated monosaccharides
A corona charged aerosol
detector measures analytes as
particles. Accordingly, the
baseline will be significantly
influenced by components
eluted from the column.
The polymer-based amino
column, NH2P series
eliminates column originated
components, and thus enables
a stable baseline with lower
noise level.
1
2
3
4
Sample : 5μL
1. 2-Aminopyridine
2. PA-Rhamnose
3. PA-Fucose
4. PA-Ribose
5. PA-Xylose
6. PA-N-Acetylglucosamine
7. PA-N-Acetylgalactosamine
8. PA-Mannose
9. PA-Glucose
10. PA-Galactose
7
1
10
8
9
2
5
0.1pmol/μL
1pmol/μL
1pmol/μL
1pmol/μL
1pmol/μL
1pmol/μL
1pmol/μL
1pmol/μL
1pmol/μL
1pmol/μL
6
5
Silica-based amino column
(4.6mmI.D. x 250nm)
0
20 min
NH2P-50 4E
(4.6mmI.D. x 250nm)
10
15 min
Column
: Shodex Asahipak NH2P-50 4E
Silica-based amino column from other manufacturer
Silica-based amide column from other manufacturer
Eluent
: H2O/CH3CN=20/80
Flow rate
: 1.0mL/min
Detector
: Corona charged aerosol
Column temp : 30˚C(NH2P-50 4E, Silica-based amino column)
80˚C(Silica-based amide column)
0
20
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Sample : 0.05% each, 20μL
1. Stevioside
2. Rebaudioside A
2
1
Sample : 20μL
1. Vitamin B6
2. Nicotinamide
3. Vitamin B12
4. Nicotinic acid
5. Folic acid
6. Vitamin C
Ascorbic acid and erythorbic acid
Sample : 5μg/mL each, 10μL
50μg/mL
10μg/mL
10μg/mL
10μg/mL
10μg/mL
10μg/mL
1. Erythorbic acid
O=C
HOC
O
HOC
HC
HCOH
CH2OH
4
2
60 min
Shodex Asahipak NH2P-50 4E
H3PO4/H2O/CH3CN=1/14/85
0.5mL/min
Fluorescence(Ex. : 310nm, Em. : 380nm)
40˚C
Simultaneous analysis of water-soluble
vitamins
Stevioside and rebaudioside A
40
Columns for Polymer-based Hydrophilic Interaction Chromatography (HILIC)
3
1
2. L-Ascorbic acid
O=C
HOC
O
HOC
HC
HOCH
2
CH2OH
6
1
5
3
0
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
Shodex Asahipak NH2P-50 4E
H2O/CH3CN=25/75
1.0mL/min
UV(210nm)
30˚C
15
min
0
1
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
2
3
4
5
min
Shodex Asahipak NH2P-50 4E
40mM H3PO4 aq./CH3CN=45/55
1.0mL/min
UV(254nm)
40˚C
0
5
10 min
Column
Eluent
: Shodex Asahipak NH2P-50 4E
: 20mM NaH2PO4 + 30mM H3PO4 aq.
/CH3CN=20/80
Flow rate
: 1.0mL/min
Detector
: UV(254nm)
Column temp. : 30˚C
13
Standard Analysis
Columns
Columns for Silica-based Reversed Phase,
HILIC and Normal Phase Chromatography
[ODS columns]
Features
Columns for Silica-based Reversed Phase, HILIC and Normal Phase Chromatography
F
This ODS column has been sold for over 30 years with reliable quality
C18M
Monomeric type ODS column, fully end capped high purity silica (99.99% or higher)
C18P
Polymeric type ODS column, fully end capped high purity silica (99.99% or higher)
Excellent acid tolerance
Advantageous for separating planar and nonplanar compounds from each other
Preparative
Columns
p.79
See also page 8, "Features and applications of different packing materials used for columns for reversed phase, hydrophilic interaction and
normal phase chromatography".
Standard columns
Product Code
Product Name
Plate Number
(TP/column)
Functional
Group
Particle Size Carbon Load Pore Size Column Size (mm)
(µm)
(%)
(Å)
I.D. x Length
Shipping Solvent
F6604112
ODSpak F-411
≥ 8,000
Octadecyl
5
14
100
4.6 × 150
H2O/CH3OH=20/80
F6605110
ODSpak F-511
≥ 14,000
Octadecyl
5
14
100
4.6 × 250
H2O/CH3OH=20/80
F6604113
ODSpak F-411/S
≥ 10,000
Octadecyl
3
14
100
4.6 × 100
H2O/CH3OH=20/80
F6650040
Silica C18M 4D
≥ 10,000
Octadecyl
5
16
100
4.6 × 150
H2O/CH3OH=30/70
F6650041
Silica C18M 4E
≥ 16,000
Octadecyl
5
16
100
4.6 × 250
H2O/CH3OH=30/70
F6650042
Silica C18M 2D
≥ 9,000
Octadecyl
5
16
100
2.0 × 150
H2O/CH3OH=40/60
F6650045
Silica C18P 4D
≥ 10,000
Octadecyl
5
17
100
4.6 × 150
H2O/CH3OH=30/70
F6650046
Silica C18P 4E
≥ 16,000
Octadecyl
5
17
100
4.6 × 250
H2O/CH3OH=30/70
F6650047
Silica C18P 2D
≥ 9,000
Octadecyl
5
17
100
2.0 × 150
H2O/CH3OH=40/60
Base Material : Silica
[Other silica-based columns]
Features
E-411
Silica gel packed column without any chemical modification
5SIL
Uses high purity silica (99.99% or higher)
Like E-411, suitable for normal phase analysis using a nonpolar organic solvent
Preparative
Columns
p.79
Use when the retention capacity of C18 is too strong or that of 5C4 is too week
Applicable to ion pair chromatography, because of its rapid mass transfer and equilibration
Preparative
Columns
p.79
Preparative
Columns
p.79
5C8
5C4
Use when the retention capacity of C18 or C8 is too strong
5CN
Utilizes reversed phase interaction and π-electron interaction to separate regioisomers, which typically
cannot be separated with ODS, C8, or C4 columns
5NPE,
5PYE
Utilizes several types of interactions based on π-electrons to separate structural isomers
5NH
Suitable for saccharides analysis by hydrophilic interaction chromatography (HILIC)
Preparative
Columns
p.79
See also page 8, "Features and applications of different packing materials used for columns for reversed phase, hydrophilic interaction and
normal phase chromatography".
Standard columns
14
Product Code
Product Name
Plate Number
(TP/column)
Functional
Group
Particle Size Carbon Load Pore Size Column Size (mm)
(µm)
(%)
(Å)
I.D. x Length
F6604110
Silicapak E-411
≥ 8,000
−
5
−
50
4.6 × 150
F6650050
Silica 5SIL 4D
≥ 9,000
−
5
−
100
4.6 × 150
C6H14/C2H5OH=95/5
F6650051
Silica 5SIL 4E
≥ 15,000
−
5
−
100
4.6 × 250
C6H14/C2H5OH=95/5
F6650052
Silica 5C8 4D
≥ 9,000
Octyl
5
10
100
4.6 × 150
H2O/CH3OH=34/66
F6650053
Silica 5C8 4E
≥ 15,000
Octyl
5
10
100
4.6 × 250
H2O/CH3OH=34/66
F6650054
Silica 5C4 4D
≥ 9,000
Butyl
5
7
100
4.6 × 150
H2O/CH3OH=45/55
F6650055
Silica 5C4 4E
≥ 15,000
Butyl
5
7
100
4.6 × 250
H2O/CH3OH=45/55
F6650058
Silica 5CN 4D
≥ 7,000
Cyanopropyl
5
−
100
4.6 × 150
H2O/CH3OH=60/40
F6650059
Silica 5CN 4E
≥ 12,000
Cyanopropyl
5
−
100
4.6 × 250
H2O/CH3OH=60/40
F6650062
Silica 5NPE 4D
≥ 8,000
Nitrophenylethyl
5
−
100
4.6 × 150
H2O/CH3OH=45/55
F6650063
Silica 5PYE 4D
≥ 7,000
Pyrenylethyl
5
−
100
4.6 × 150
H2O/CH3OH=30/70
F6650060
Silica 5NH 4D
≥ 5,000
Aminopropyl
5
−
100
4.6 × 150
H2O/CH3CN=5/95
F6650061
Silica 5NH 4E
≥ 8,000
Aminopropyl
5
−
100
4.6 × 250
H2O/CH3CN=5/95
*Contact Shodex or our distributors near you for customized columns.
Shipping Solvent
IPA/Dichloromethane/n-Hexane
=1/50/49
Base Material : Silica
Sample : 10μL
1. Mandelic acid
2. Hippuric acid
3. o-Methylhippuric acid
4. p-Methylhippuric acid
5. m-Methylhippuric acid
6. Creatinine
Standards
2
17μg/mL
17μg/mL
17μg/mL
17μg/mL
17μg/mL
33μg/mL
TFA method
2
4
3
5
3
1
6
4
6
2
1
10 min
5
0
5
0
10 min
Column
Eluent
: Shodex ODSpak F-411
: [20mM H3PO4 + 20mM β-Cyclodextrin(pH2.5)]/CH3CN=88/12
+ 4.5mM Sodium dodecyl sulfate
Flow rate
: 1.0mL/min
Detector
: UV(225nm)
Column temp. : 50˚C
Trichothecene mycotoxins
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Coenzyme Q10
10
15
20 min
Shodex Silica C18M 4E
H2O/CH3CN/CH3OH=60/10/30
1.0mL/min
Fluorescence(Ex. : 365nm, Em. : 450nm)
40˚C
Catechins in green tea
Sample : 20μL
1. Nivalenol (INIV)
2. Deoxynivalenol (DON)
Sample : 5μL
1. Coenzyme Q10 1mg/mL
Sample :
1. (-)-Epigallocatechin
2. (-)-Catechin
3 (-)-Epigallocatechin gallate
4. (-)-Catechin gallate
3
* 1 ppm each of DON and
NIV were added to wheat sample
1
1.0μg
1.0μg
0.5μg
1.0μg
4
Reference :
Shoku-An No. 0717001
(July 17, 2003, Japan)
Test method of deoxynivalenol
1
2
1
2
0
0
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
5
10
15
min
15 min
Shodex Silica C18M 4E
H2O/CH3CN/CH3OH=90/5/5
1.0mL/min
UV(220nm)
40˚C
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Benzylpyridine isomers
Shodex Silica C18P 4D
CH3OH/C2H5OH=13/7
1.2mL/min
UV(275nm)
35˚C
20
30
40 min
Shodex Silica C18P 4D
0.1% H3PO4 aq./CH3OH=20/80
1.0mL/min
UV(254nm)
30˚C
Simultaneous analysis of vitamin E homologs
Sample : 20μL
1. α-Tocopherol 5μg/mL
2. α-Tocotrienol 10μg/mL
3. β-Tocopherol 5μg/mL
4. γ-Tocopherol 5μg/mL
5. γ-Tocotrienol 10μg/mL
6. δ-Tocopherol 5μg/mL
7. δ-Tocotrienol 10μg/mL
Sample :
1. Naphthalene
2. 1,5-Dimethylnaphthalene
3. 1,5-Dinitronaphthalene
4. 1,8-Dinitronaphthalene
1
10
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Dinitronaphthalene isomers
Sample :
1. 2-Benzylpyridine
2. 3-Benzylpyridine
3. 4-Benzylpyridine
0
Columns for Silica-based Reversed Phase, HILIC and Normal Phase Chromatography
0
Reference :
"Test Methods Related to
Total Aflatoxin" in Notice
Shoku-An No. 0816-1
(August 16, 2011, Japan)
Sample :
5μg/L each, 20μL
1. Derived Aflatoxin G1
2. Derived Aflatoxin B1
3. Aflatoxin G2
4. Aflatoxin B2
Urine(10-fold diluted)
Standard Analysis
Columns
Aflatoxins
Hippuric acids in urine
6
4
2
1
2
3
2
1
3
3
0
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
15
20
5
7
4
25
min
Shodex Silica 5PYE 4D
20mM KH2PO4 aq./CH3OH=40/60
1.0mL/min
UV(254nm)
30˚C
0
5
10
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Shodex Silica 5NPE 4D
H2O/CH3OH=30/70
1.0mL/min
UV(254nm)
30˚C
15
min
0
5
10
15
min
Column
Eluent
: Shodex Silica 5SIL 4D
: n-Hexane/Isopropanol/Acetic acid
=1000/6/5
Flow rate
: 1.0mL/min
Detector
: Fluorescence(Ex. : 298nm, Em. : 325nm)
Column temp. : 30˚C
15
Standard Analysis
Columns
Columns for Ligand Exchange Chromatography
* A list of elution volume of saccharides for Shodex columns is available.
Please refer to our website (www.shodex.de) or technical notebook (No.2 and 3).
Features
SC1011, 1821,
SP0810
KS-801, 802
Separates saccharides by combination of ligand exchange and size exclusion modes
Three types of counter ions are available: Ca2+, Pb2+, and Na+
[KS-801, 802]
Only water is required for the analysis of neutral sugars
Note
book
KS-803~807
Preparative
Columns
No.6, 40, 43
Columns for Ligand Exchange Chromatography
No.3
News
p.80
[KS-803~806]
Preparative
Columns
No.8, 13
Separates by combination of ligand exchange and HILIC modes
DC-613 can analyze sugars without removing sodium salts in the sample
SZ5532 is recommended for the separation of disaccharides or trisaccharides
SC1211 is suitable for separation of sugar alcohols
Note
book
MN-431,
SC1011-7F
News
Suitable for separation of polysaccharides by size exclusion mode
Can be used in tandem e.g., KS-802 and KS-801
Only water is required for the analysis of neutral sugars
Note
book
DC-613,
SZ5532,
SC1211
No.3
[DC-613]
Preparative
Columns
No.2
p.80
p.80
MN-431 is for the analysis of mannitol in conformity with USP
SC1011-7F is for the analysis of sugar alcohols and saccharides in conformity with EP
Ca-type ligand exchange chromatography columns
Standard columns
Ligand exchange and size exclusion
Product Code
Product Name
Plate Number
(TP/column)
Functional Group
(Counter Ion)
F6378102
SUGAR SC1011
≥ 13,000
F6378103
SUGAR SC1821
≥ 13,000
Sulfo (Ca2+)
Sulfo (Ca2+)
F6700090
SUGAR SC-LG
(guard column)
F6378105
SUGAR SP0810
Particle
Size
(µm)
Column Size
(mm)
I.D. x Length
Shipping Solvent
1,000
6
8.0 × 300
H2O
10,000
6
8.0 × 300
H2O
10
6.0 × 50
H2O
7
8.0 × 300
H2O
Exclusion Limit
(Pullulan)
−
≥ 11,000
Sulfo (Ca2+)
Sulfo (Pb2+)
−
F6700081
SUGAR SP-G
(guard column)
Sulfo (Pb2+)
F6378010
SUGAR KS-801
≥ 17,000
F6378020
SUGAR KS-802
≥ 17,000
Sulfo (Na+)
Sulfo (Na+)
F6378025
SUGAR KS-803
≥ 17,000
F6378035
SUGAR KS-804
≥ 17,000
F6378050
SUGAR KS-805
≥ 9,000
F6378060
SUGAR KS-806
F6700020
SUGAR KS-G
F6378070
SUGAR KS-807
F6700021
SUGAR KS-807G
1,000
10
6.0 × 50
H2O
1,000
6
8.0 × 300
H2O
10,000
6
8.0 × 300
H2O
50,000
6
8.0 × 300
H2O
400,000
7
8.0 × 300
H2O
5,000,000
17
8.0 × 300
H2O
(50,000,000)*
Sulfo (Na+)
Sulfo (Na+)
Sulfo (Na+)
Sulfo (Na+)
≥ 9,000
17
8.0 × 300
H2O
−
10
6.0 × 50
H2O
≥ 4,000
Sulfo (Na+)
Sulfo (Na+)
(200,000,000)*
30
8.0 × 300
H2O
(guard column)
Sulfo (Na+)
−
30
8.0 × 50
H2O
(guard column)
( )* Estimated value
Base Material : Styrene divinylbenzene copolymer
Ligand exchange and HILIC
Product Code
Product Name
F7001003
RSpak DC-613
F6700170
RSpak DC-G
F7001300
SUGAR SZ5532
F6700110
SUGAR SZ-G
F7001400
SUGAR SC1211
F6700120
SUGAR SC-G
Plate Number
(TP/column)
Functional Group
(Counter Ion)
Particle
Size
(µm)
Pore Size
(Å)
Column Size
(mm)
I.D. x Length
≥ 5,500
Sulfo (Na+)
Sulfo (Na+)
6
100
6.0 × 150
H2O/CH3CN=30/70
10
−
4.6 × 10
H2O/CH3CN=30/70
Sulfo (Zn2+)
Sulfo (Zn2+)
6
100
6.0 × 150
H2O/CH3CN=30/70
6
−
4.6 × 10
H2O/CH3CN=30/70
Sulfo (Ca2+)
Sulfo (Ca2+)
6
50
6.0 × 250
H2O/CH3CN=75/25
10
−
4.6 × 10
H2O/CH3CN=75/25
(guard column)
≥ 5,500
(guard column)
≥ 5,500
(guard column)
Shipping Solvent
Base Material : Styrene divinylbenzene copolymer
For the United States Pharmacopeia (USP)
Product Code
Product Name
Functional Group
(Counter Ion)
Particle Size
(µm)
Column Size (mm)
I.D. x Length
Shipping Solvent
F6379230
USPpak MN-431
Sulfo (Ca2+)
8
4.0 × 250
H2O
See p.71 for USP (Ver.35) Column List.
Base Material : Styrene divinylbenzene copolymer
For the European Pharmacopeia (EP)
Product Code
16
F6379300
Product Name
EP SC1011-7F
Functional Group
(Counter Ion)
Particle Size
(µm)
Sulfo (Ca2+)
8
Column Size (mm)
I.D. x Length
Shipping Solvent
7.8 × 300
H2O
Base Material : Styrene divinylbenzene copolymer
*Contact Shodex or our distributors near you for customized columns.
O
O
OH(eq)
HO(ax)
OH(eq)
HO
(eq)
OH(ax)
OH(ax)
M+
O3S
M+
O3S
triplet
M+ : Metal ion
pair
Elution volume of saccharides analysis with various columns
[Partial list only; refer to our website for complete list]
Elution Volume (mL)
Substances
Elution Volume (mL)
SP0810 SC1011 KS-801 SZ5532 NH2P-50 SC1211 DC-613
4E
Substances
SP0810 SC1011 KS-801 SZ5532 NH2P-50 SC1211 DC-613
4E
Arabinose
10.42
8.91
8.21
5.11
6.18
5.56
5.75
D-Mannose
10.72
8.17
7.64
5.83
7.84
5.01
6.72
D-Arabitol
15.86
11.33
7.63
7.27
6.29
8.16
5.81
Melibiose
8.16
6.45
5.98
11.69
14.70
4.23
14.83
Dulcitol
20.18
12.76
7.40
9.46
7.45
11.28
7.33
Nystose
6.38
5.45
4.93
20.05
31.90
*
—
meso-Erythritol
12.70
10.09
7.86
5.73
5.43
6.27
4.84
Palatinit
5.90
2peaks
12.73
D(-)-Fructose
11.05
8.85
7.71
5.37
6.75
5.90
6.19
Palatinose
7.84
6.45
5.89
8.08
12.12
3.99
9.81
D(+)-Fucose
2peaks 2peaks
2peaks 2peaks
10.48
8.84
8.09
4.50
5.43
4.96
4.81
Panose
7.14
5.78
5.32
16.87
25.60
*
23.14
D(+)-Galactose
9.74
7.98
7.58
6.46
8.10
4.98
7.28
D(+)-Raffinose
7.14
5.78
5.29
16.36
20.25
*
19.11
Gentiobiose
7.22
6.08
5.75
10.50
16.36
*
14.45
Rhamnose
9.77
8.23
7.37
3.93
5.52
4.43
4.09
Glucose
8.63
7.30
7.17
5.87
8.61
4.76
6.83
D(-)-Ribose
19.35
13.66
9.04
4.82
5.45
8.64
5.30
12.77
8.86
7.99
12.63
9.96
7.87
15.80
D(-)-Sorbitol
21.61
13.31
7.42
9.79
7.09
11.88
7.27
Isomaltose
7.68
6.26
5.95
10.57
15.18
*
13.82
Sorbose
9.67
8.03
7.38
5.12
7.35
4.92
5.91
Isomaltotriose
7.09
5.75
5.34
21.17
27.55
*
32.02
Stachyose
6.82
5.57
4.97
—
36.22
*
—
1-Kestose
6.79
5.75
5.26
13.09
20.11
*
—
Sucrose
7.54
6.29
5.87
7.91
11.87
*
8.68
myo-Inositol
7.56
6.21
5.88
9.65
14.82
*
11.47
α-D-Talose
21.33
12.59
8.76
5.69
6.47
8.51
6.32
Lactitol
13.27
8.09
6.13
16.35
11.82
6.67
14.04
Trehalose
7.62
6.27
5.78
10.85
13.25
*
11.49
Lactose
8.05
6.51
5.99
10.12
13.27
4.07
11.69
Trehalulose
8.92
6.95
6.10
9.54
11.68
4.78
11.38
Kojibiose
9.13
6.99
6.19
9.16
10.72
4.65
10.80
Xylitol
19.87
13.14
7.94
7.77
6.10
10.16
6.19
Maltitol
12.23
8.26
6.03
13.04
11.82
6.77
11.81
Xylobiose
8.16
6.68
6.40
5.65
9.05
*
6.71
Maltose
7.85
6.34
5.94
8.67
14.24
*
10.61
D(+)-Xylose
9.21
7.90
7.71
4.55
6.58
4.48
5.21
Maltotriose
7.48
5.89
5.38
13.79
24.96
*
17.88
D-Xylulose
10.64
9.02
8.04
4.06
5.41
5.07
4.56
15.80
11.10
7.23
8.75
7.39
9.03
6.84
Lactulose
Mannitol
(—) Not detected
Columns for Ligand Exchange Chromatography
Saccharides exhibits an energy-stable chair conformation in 5-membered ring (furanose) or 6-membered ring (pyranose)
forms. Since hydroxyl groups on each carbon can take either equatorial or axial position, even two saccharides having the
same molecular structures may have different three-dimensional configurations. Ligand exchange mode separates
saccharides using this configuration difference of the complex formed between saccharides’ hydroxyl groups and metal
ions. As shown in the left figure, saccharides having a larger number of ax-eq-ax configuration (triplet) units form stronger
complexes with metal ions. Meanwhile, as right figure shows, saccharides lacking such a triplet structure form complexes
with ax-eq (pair) hydroxyl group. As the unit number of this pair structure increases, saccharides become more potent
forming complexes with metal ions. The complex formation capacity also differs depending on modified metal ions.
Standard Analysis
Columns
Mechanism of saccharide separation using the ligand exchange mode
(—) Not detected
(*) Overlap with solvent peak
(*) Overlap with solvent peak
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
SUGAR SP0810, SC1011, KS-801
H2O
1.0mL/min
RI
80˚C
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
SUGAR SZ5532
H2O/CH3CN=25/75
1.0mL/min
RI
60˚C
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
SUGAR SC1211
H2O/CH3CN=65/35
1.0mL/min
RI
70˚C
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Asahipak NH2P-50 4E
H2O/CH3CN=25/75
1.0mL/min
RI
30˚C
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
RSpak DC-613
H2O/CH3CN=25/75
1.0mL/min
RI
70˚C
17
Anomer separation of saccharides affects the
chromatogram.
When using a SUGAR column to analyze saccharides, the
analysis at high temperatures would suppress the influence of
anomer separation, resulting in proper chromatograms.
Glucose
Mannose
Xylose
Galactose
Sample :
0.5% each, 10μL
107
106
Arabinose
29˚C
0
Columns for Ligand Exchange Chromatography
Molecular Weight (Pullulan)
Standard Analysis
Columns
Calibration curves for KS-800 series using pullulan
Anomer separation of saccharides
10
20 min
Glucose
0
10
20 min
0
Mannose
10
20 min
Xylose
0
10
20 min
Galactose
0
10
20 min
Arabinose
KS
-8
06
KS
-80
5
105
KS
-8
04
104
KS
-8
03
KS
-80
2
103
KS801
70˚C
102
4
0
10
20 min
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
0
10
20 min
0
10
20 min
0
10
20 min
0
10
6
Shodex SUGAR SC1011
H2O
0.7mL/min
RI
29˚C, 70˚C
Column
:
Eluent
:
Detector
:
Column temp. :
Hydrolyzed starch
Saccharides in wood (model)
Dp-1
Sample : 20μL
Hydrolyzed starch 1%
Sample : 5μL
1. Cellobiose
2. Glucose
3. Xylose
4. Galactose
5. Arabinose
6. Mannose
Dp-5
8
10
12
Elution volume (mL)
20 min
1.0%
1.5%
0.5%
0.5%
0.5%
1.0%
Shodex SUGAR KS-800 series
H2O
RI
80˚C
Ketohexoses
Sample : 0.025% each, 10μL
1. Sorbose
2. Fructose
3. Tagatose
4. Psicose
2
Dp-10
1
1
2
6
3 4
3
5
4
5
15
10
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
15
20
min
20 min
Shodex SUGAR KS-802 x 2
H2O
1.0mL/min
RI
80˚C
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Shodex SUGAR SP0810
H2O
0.6mL/min
RI
85˚C
Sample : 20μL, 0.1% each
1. Raffinose
2. Sucrose
3. Galactinol
4. Galactose
5. myo-Inositol
Sample : 0.1% each, 20μL
1. Verbascose
2. Stachyose
3. Raffinose
4. Sucrose
5. Pinitol
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Saccharides related to raffinose
biosynthesis
Oligosaccharides in soybean
0
10
20
min
Shodex SUGAR SP0810
H2O
1.0mL/min
RI
80˚C
Acesulfame K and sucralose
Sample : 20μL
1. Acesulfame K 0.1%
2. Sucrose
0.5%
3. Glucose
0.5%
4. Unknown from Acesulfame K
5. Fructose
0.5%
6. Sucralose
0.1%
2
5
1
5
15
2
3
4
4
3
2
3
5
1
1
6
4
10
18
15
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
20
25
30
min
Shodex SUGAR KS-802 + KS-801
H2O
0.6mL/min
RI
85˚C
0
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
5
Shodex SUGAR SC1011
H2O
1.0mL/min
RI
80˚C
10 min
0
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
15
Shodex SUGAR SC1011
10mM CaSO4 aq.
0.6mL/min
RI
80˚C
20
min
Maltose and isomaltose
Sample : 0.5% each, 10μL
1. Fructose
2. Glucose
3. Sucrose
4. Turanose
5. Lactose
Saccharides and sugar alcohols
Sample : 0.5% each, 20μL
1. Glucose
2. Maltose
3. Isomaltose
4. Maltotriose
5. Isomaltotriose
1
1 2
Sample : 0.5% each, 20μL
1. Rhamnose
2. Xylose
3. Arabinose
4. Fructose
5. Glucose
6. Galactose
7. Arabitol
8. Xylitol
9. Mannitol
10. Sorbitol
4
Standard Analysis
Columns
Sucrose and turanose
1 2
4
4 5
5
2
5
3
3
3
7
8
Columns for Ligand Exchange Chromatography
6
9
10
0
5
10
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
15
20
25
30
35
min
0
Shodex SUGAR SZ5532
H2O/CH3CN=20/80
0.6mL/min
RI
60˚C
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
15
20 min
0
Shodex SUGAR SZ5532
H2O/CH3CN=25/75
1.0mL/min
RI
60˚C
Saccharides in presence of sodium salt
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
Shodex SUGAR SZ5532
H2O/CH3CN=20/80
1.0mL/min
RI
65˚C
Pinitol
Since DC-613 uses Na+ as counter ion, it enables the analysis of saccharides in
sodium salt containing samples without desalting. Acid hydrolyzed sample can
be analyzed after neutralizing the remaining acid, such as hydrochloric or sulfuric
acid, with sodium hydroxide.
Sample : 0.1% each, 10μL
1. Pinitol
2. Fructose
3. Glucose
4. Sucrose
5. chiro-Inositol
6. myo-Inositol
Sample : 0.1% each, 10μL
1. Xylose
2. Sucrose
3. Cellobiose
4. Lactose
Solvent for sample
preparation
1
1% NaCl aq./CH3CN
=50/50
15 min
2
1
4
3
2
1% Na2SO4 aq./CH3CN
=50/50
5
3
6
4
H2O/CH3CN
=50/50
0
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
5
Shodex RSpak DC-613
H2O/CH3CN=30/70
0.8mL/min
RI
50˚C
10
15
min
* High concentration of acetonitrile is
used for saccharide analysis by DC-613.
Pay attention to possible salt deposition
when analyzing with high salt
concentration samples.
Moisturizing components
15
20
min
Shodex RSpak DC-613
H2O/CH3CN=25/75
1.0mL/min
RI
70˚C
1
2
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
According to the USP (United States Pharmacopeia) method, mannitol
should be analyzed using a column which can separate mannitol and
sorbitol with a resolution equal to or greater than 2.0.
MN-431 is a column specially designed for mannitol analysis, which
satisfies this criteria.
Sample : 0.5% each, 10μL
1. Mannitol
2. Sorbitol
4
0
5
Mannitol analysis with USP method
1
Sample : 0.1% each, 20μL
1. 1,3-Propanediol
2. Propylene glycol
3. Ethylene glycol
4. Glycerin
5. meso-Erythritol
0
5
Shodex SUGAR SC1211
H2O/CH3CN=60/40
0.6mL/min
RI
40˚C
10
3
2
5
15
min
0
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
2
4
Shodex USPpak MN-431
H2O
0.5mL/min
RI
60˚C
6
8
min
19
Standard Analysis
Columns
Columns for Ion Exclusion Chromatography
Features
SH1011, 1821
Columns for simultaneous analysis of saccharides and organic acids
Separates neutral sugars in size exclusion mode and organic acids in ion exclusion mode
Suitable for the analysis of uronic and aldonic acids
Note
book
Columns for Ion Exclusion Chromatography
KC-811
No.3
News
No.25, 40, 43
Columns for the analysis of organic acids
Ion exclusion mode (+ reversed phase mode)
Highly selective detection with post column method
KC-811 6E is suitable for the analysis of cyanide ions and cyanogen chloride in
accordance with the Japanese Water Supply Act
Note
book
No.3
News
No.6
Preparative
Columns
p.80
Standard columns
For simultaneous analysis of saccharides and organic acids
Product Code
Product Name
Plate Number
(TP/column)
Functional
Group
Exclusion Limit
(Pullulan)
Particle
Size
(µm)
Column Size
(mm)
I.D. x Length
Shipping Solvent
F6378100
SUGAR SH1011
≥ 17,000
Sulfo
1,000
6
8.0 × 300
H2O
F6378101
SUGAR SH1821
≥ 17,000
Sulfo
10,000
6
8.0 × 300
H2O
F6700080
SUGAR SH-G
(guard column)
Sulfo
−
10
6.0 × 50
H2O
Base Material : Styrene divinylbenzene copolymer
For organic acids, cyanide ions and cyanogen chloride
Product Code
Product Name
Plate Number
(TP/column)
Functional
Group
Particle Size
(µm)
Column Size
(mm)
I.D. x Lenngth
Shipping Solvent
F6378030
RSpak KC-811
≥ 17,000
Sulfo
6
8.0 × 300
H2O
F6378033
RSpak KC-811 6E
≥ 13,000
Sulfo
6
6.0 × 250
H2O
F6700030
RSpak KC-G
(guard column)
Sulfo
10
6.0 × 50
H2O
F6700010
RSpak KC-LG
(guard column)
Sulfo
13
8.0 × 50
H2O
* As a guard column, use KC-LG for samples with relatively high impurity
and KC-G for samples with relatively low impurity.
20
*Contact Shodex or our distributors near you for customized columns.
Base Material : Styrene divinylbenzene copolymer
Cello-oligosaccharides and furfurals
Sample : 0.05% each, 20μL
1. Maltotetraose
2. Maltotriose
3. Maltose
4. Glucose
5. Lactic acid
6. Glycerol
7. Acetic acid
8. Methanol
12 3
4
9. Ethanol
Sample : 0.1% each, 10μL
1. Cellopentaose
2. Cellotetraose
3. Cellotriose
4. Cellobiose
5. Glucose
6. Glyceric acid
7. Acetic acid
8. 5-HMF
9. Furfural
4
12
5
8
6
6
5
3
7
7
General organic acids
Sample :
1. Citric acid
2. Tartaric acid
3. Pyruvic acid
4. Malic acid
5. Succinic acid
2 4
6. Glycolic acid
7. Lactic acid
8. Fumaric acid
1
9. Acetic acid
10. Levulinic acid
11. Pyroglutamic acid
12. Propionic acid
13. Isobutyric acid
14. n-Butyric acid
Standard Analysis
Columns
Maltooligosaccharides, organic acids
and ethanol
9
5
6
8
12
7
10
13 14
11
3
0
5
10
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
15
20
25
min
0
Shodex SUGAR SH1821
0.5mM H2SO4 aq.
0.6mL/min
RI
75˚C
15
20
Eluent = 2mM HClO4 aq.
6
30
7
35
40
45
min
Column
Eluent
Flow rate
Detector
20
:
:
:
:
Glucronolactone and organic acids
Sample :
1. Cl-,etc.
2. Oxalic acid
3. Maleic acid
4. Citric acid
5. Malic acid
6. Vitamin C
7. Succinic acid
8. Formic acid
9. Acetic acid
5
30
min
Shodex RSpak KC-811 x 2
6mM HClO4 aq.
1.0mL/min
VIS(430nm)
post column method
Column temp. : 50˚C
Organic acids and vitamin C in fruit juice
8
10
Shodex SUGAR SH1821
2mM H2SO4 aq.
0.6mL/min
RI
60˚C
4
5
25
Sample :
1. Succinic acid
2. Lactic acid
3. Formic acid
4. Acetic acid
5. Propionic acid
6. Isobutyric acid
7. n-Butyric acid
8. Isovaleric acid
9. n-Valeric acid
1
4
10
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Hydrophobic organic acids
2 3
5
Sample : 20μL
1. Citric acid
2. Malic acid
3. Glucronolactone
4. Glycerin
5. Ethanol
0.01%
0.01%
0.01%
0.01%
0.05%
5
Columns for Ion Exclusion Chromatography
9
9
8
3
9
4
1
2
20
25
30
35
40 min
To shorten analysis time
Eluent = 2mM HClO4 aq./CH3CN=90/10
1
5
2
3
6
7
8
9
15
10
20 min
0
15
20
25
5
10
15
min
30 min
Column
Eluent
Flow rate
Detector
Shodex RSpak KC-LG + KC-811 x 2
1mM HClO4 aq.
1.0mL/min
VIS(430nm)
post column method
Column temp. : 40˚C
Column
Flow rate
Detector
: Shodex RSpak KC-LG + KC-811 x 2
: 1.0mL/min
: VIS(430nm)
post column method
Column temp. : 47˚C
:
:
:
:
Shodex RSpak KC-811
3mM HClO4 aq.
1.0mL/min
RI
40˚C
Cyanide ion and cyanogen chloride with
post column method
Organic acids in sake
Sample : 100μL
1. Phosphoric acid etc.
2. Citric acid
3. Pyruvic acid
4. Malic acid
5. Succinic acid
6. Lactic acid
7. Fumaric acid
5
8. Acetic acid
9. Pyroglutamic acid
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
CN-
Fine rice wine
Fermented liquor
6
Sample : 10μg/L each, 100μL
Pure rice wine
CNCl
5
4
5
6
6
4
1
1
1
4
2
2
3
0
5
Column
Eluent
Flow rate
Detector
10
15
:
:
:
:
3
25
min
0
5
10
Shodex RSpak KC-LG + KC-811 x 2
4.8mM HClO4 aq.
1.0mL/min
VIS(430nm)
post column method
Column temp. : 63˚C
15
4
6
8
10 min
2
4
6
8
10 min
7
7
20
2
9
8
3
7
20
2
8 9
8 9
25
min
0
5
10
15
20
25
min
Column
Eluent
Reagent A
Reagent B
:
:
:
:
Shodex RSpak KC-811 6E
1.0mM H2SO4 aq.
Chloramine T solution
4-Pyridinecarboxylic acid-Pyrazolone
solution
Flow rate
: (Eluent) 1.0mL/min
(Reagent) 0.5mL/min each
Detector
: VIS(638nm)
Column temp. : 40˚C
Reaction temp. : (Reagent A) 40˚C
(Reagent B) 80˚C
21
Standard Analysis
Columns
Columns for Ion Chromatography (Anion Analysis)
Features
NI-424, I-524A
Columns for anion analysis with non-suppressor method
NI-424 supports simultaneous analysis of fluoride and phosphate ions
Columns for anion analysis with suppressor method
SI-90, SI-50
Suitable for the quantitative analysis of fluoride ion
Columns for Ion Chromatography (Anion Analysis)
SI-50 separates target inorganic anions from organic acids
Not interfered by the system peak derived from carbonate
Columns for the analysis of oxyhalides
NEW SI-35
SI-52
SI-35 supports rapid analysis of oxyhalides and general inorganic ions
SI-52 supports simultaneous analysis of oxyhalides and general inorganic ions
Standard columns
For anions (non-suppressor method)
Product
Code
Product Name
F6995243
IC NI- 424
F6709616
IC NI-G
F6995240
IC I-524A
F6700400
IC IA-G
Particle Size Column Size
(mm)
(µm)
I.D. x Length
Plate Number
(TP/column)
Functional Group
≥ 5,000
Quaternary ammonium
5
4.6 × 100
8mM 4-Hydroxybenzoic acid + 2.8mM Bis-Tris
+ 2mM Phenylboronic acid + 0.005mM CyDTA aq.
(guard column) Quaternary ammonium
5
4.6 × 10
8mM 4-Hydroxybenzoic acid + 2.8mM Bis-Tris
+ 2mM Phenylboronic acid + 0.005mM CyDTA aq.
Quaternary ammonium
12
4.6 × 100
2.5mM Phthalic acid aq.
(guard column) Quaternary ammonium
12
4.6 × 10
≥ 2,000
Shipping Solvent
2.5mM Phthalic acid aq.
Base Material : Polyhydroxymethacrylate
For anions (suppressor method)
Product
Code
F6995244
Product Name
IC SI-90 4E
F6709620
IC SI-90G
F6995245
IC SI-50 4E
F6709625
IC SI-50G
Column Size
Particle Size
(mm)
(µm)
I.D. x Length
Plate Number
(TP/column)
Functional Group
≥ 5,000
Quaternary ammonium
9
4.0 × 250
1.8mM Na2CO3 + 1.7mM NaHCO3 aq.
(guard column) Quaternary ammonium
Shipping Solvent
9
4.6 × 10
1.8mM Na2CO3 + 1.7mM NaHCO3 aq.
Quaternary ammonium
5
4.0 × 250
3.2mM Na2CO3 + 1.0mM NaHCO3 aq.
(guard column) Quaternary ammonium
5
4.6 × 10
3.2mM Na2CO3 + 1.0mM NaHCO3 aq.
≥ 10,000
Base Material : Polyvinyl alcohol
Housing Material : PEEK
For oxyhalides (suppressor method)
Product
Code
Product Name
Plate Number
(TP/column)
Functional Group
F6995290
NEW IC SI-35 4D
≥ 13,000
Quaternary ammonium
F6709627
NEW IC SI-95G
F6995260
IC SI-52 4E
F6709626
IC SI-92G
Particle Size Column Size
(mm)
(µm)
I.D. x Length
(guard column) Quaternary ammonium
Shipping Solvent
3.5
4.0 × 150
3.6mM Na2CO3 aq.
9
4.6 × 10
3.6mM Na2CO3 aq.
Quaternary ammonium
5
4.0 × 250
3.6mM Na2CO3 aq.
(guard column) Quaternary ammonium
9
4.6 × 10
3.6mM Na2CO3 aq.
≥ 14,000
Base Material : Polyvinyl alcohol
Housing Material : PEEK
Line filters for IC
Product
Code
Product Name
F8500630
IC FL-1
F8500640
IC FL-1 filter
F8500650
IC FL-2
F8500660
IC FL-2 filter
Application
Contents
For general purposes
One holder and one filter
Replacement filter for IC FL-1
5 filters
Non-metal type
One holder and one filter
Replacement filter for IC FL-2
4 filters
22
*Contact Shodex or our distributors near you for customized columns.
Sample : 20μL
1. H2PO4- 10mg/L
2. F1mg/L
3. CI1mg/L
4. NO2
5mg/L
5. Br
5mg/L
6. NO35mg/L
7. SO425mg/L
NI-424
3
4
2
5
1
NI-424 is a high performance type
column and offers twice increased
theoretical plate number than I-524A.
I-524A
<Features of NI-424>
(1) Enables the separation of H2PO4and F- ions which were difficult to
separate with I-524A.
(2) The shape of each peak is sharper,
and the separation balance is
proper. Especially, the separation
of Cl- and NO2- is improved.
1,2
3
4
7
6
5
6
7
5
10
15 min
0
: Shodex IC NI-424
: 8mM 4-Hydroxybenzoic acid
+ 2.8mM Bis-Tris + 2mM Phenylboronic acid
+ 0.005mM *CyDTA aq.
Flow rate
: 1.0mL/min
Detector
: Non-suppressed conductivity
Column temp. : 40˚C
5
10
15 min
Column
Eluent
: Shodex IC I-524A
: 2.5mM Phthalic acid
+ 2.3mM Tris(hydroxymethyl)aminomethane aq.
Flow rate
: 1.2mL/min
Detector
: Non-suppressed conductivity
Column temp. : 40˚C
*CyDTA : trans-1,2-Diaminocyclohexane-N,N,N',N'-tetra acetic acid
Anions analysis using SI-90 4E (suppressor method)
8
Sample : 20μL
1. F2mg/L
2. Cl3mg/L
3. NO25mg/L
4. Br
10mg/L
5. NO3
10mg/L
6. HCO3- 300mg/L
7. PO4315mg/L
8. SO4215mg/L
4
5
2
Perchloric acid analysis using SI-90 4E (suppressor method)
Sample : 100μL
1. Cl3mg/L
2. NO310mg/L
3. SO42- 15mg/L
4. ClO4- 10mg/L
3
2
1
1
Columns for Ion Chromatography (Anion Analysis)
0
Column
Eluent
Standard Analysis
Columns
Anions analysis with non-suppressor method (NI-424 and I-524A)
7
3
4
6
0
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
15
min
0
5
Shodex IC SI-90 4E
1.8mM Na2CO3 + 1.7mM NaHCO3 aq.
1.5mL/min
Suppressed conductivity
Room temp.(25˚C)
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Anions analysis using SI-50 4E (suppressor method)
SI-50 4E is a high performance type of SI-90 4E.
Acetic acid, formic acid, and methacrylic acid eluted
between F- and Cl-.
The carbonate system peak appears between NO2and Br- peaks.
1
8
7
5
Sample : 20μL
1. F-
2mg/L
2. Acetic acid
10mg/L
3. Formic acid
2mg/L
4. Methacrylic acid 10mg/L
5. Cl3mg/L
5mg/L
10 6. NO2
7. Br10mg/L
8. NO310mg/L
9. PO4315mg/L
10. SO4215mg/L
11. Oxalic acid
15mg/L
10
Shodex IC SI-90 4E
6mM Na2CO3 aq. + 10% CH3CN
1.0mL/min
Suppressed conductivity
25˚C
Oxyhalides and anions analysis using SI-52 4E (suppressor method)
SI-52 4E is a high resolution type column and offers 14,000 or higher
theoretical plate numbers. It supports simultaneous analysis of oxyhalides
and inorganic anions. The recommended analysis temperature is 45˚C.
4
11
9
9
1
23
5
6
4
10
23
0
5
10
15
20
25
Shodex IC SI-50 4E
3.2mM Na2CO3 + 1.0mM NaHCO3 aq.
0.7mL/min
Suppressed conductivity
25˚C
7 8
30 min
0
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Sample : 50μL
1. F2. ClO23. BrO34. Cl5. NO26. Br7. ClO -
2mg/L
1mg/L
1mg/L
10mg/L
5mg/L
10mg/L
1mg/L
3
8. Dichloroacetic acid
1mg/L
30mg/L
9. NO310. PO4315mg/L
11. SO4240mg/L
11
6
15
min
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
15
20
Shodex IC SI-52 4E
3.6mM Na2CO3 aq.
0.8mL/min
Suppressed conductivity
45˚C
25
30 min
23
Standard Analysis
Columns
Columns for Ion Chromatography (Cation Analysis)
Features
YS-50
High performance type of YK-421
Applicable to both suppressor and non-suppressor methods
Peak shape is sharper, especially for divalent cation analysis
Supports the analysis of alkylamines and transition metals
Columns for Ion Chromatography (Cation Analysis)
YK-421
Column for cation analysis with non-suppressor method
Simultaneous analysis of monovalent and divalent cations
Suitable for separation of alkylamines
Y-521
Column for cation analysis with non-suppressor method
For the separation of monovalent or divalent cations
T-521
Column for transition metal ion analysis
High sensitivity analysis is achieved by post column color reaction method
Standard columns
For cations
Particle Column Size
Size
(mm)
(µm)
I.D. x Length
Product
Code
Product
Name
Plate Number
(TP/column)
Functional
Group
Base Material
F7122000
IC YS-50
≥ 5,500
Carboxyl
Polyvinyl alcohol
5
4.6 × 125
H2O
F6700530
IC YS-G
(guard column)
Carboxyl
Polyvinyl alcohol
5
4.6 × 10
H2O
F7120012
IC YK-421
≥ 2,800
Carboxyl
Silica
5
4.6 × 125
5mM Tartaric acid + 1mM Dipicolinic
acid + 1.5g/L Boric acid aq.
F6709608
IC YK-G
(guard column)
Carboxyl
Silica
5
4.6 × 10
5mM Tartaric acid + 1mM Dipicolinic
acid + 1.5g/L Boric acid aq.
F6995210
IC Y-521
≥ 3,000
Sulfo
Styrene divinylbenzene copolymer
12
4.6 × 150
4mM HNO3 aq.
F6700230
IC Y-G
(guard column)
Sulfo
Styrene divinylbenzene copolymer
12
4.6 × 10
4mM HNO3 aq.
Plate Number
(TP/column)
Functional
Group
Base Material
≥ 3,000
Sulfo
Styrene divinylbenzene copolymer
12
4.6 × 150
3mM HNO3 aq.
(guard column)
Sulfo
Styrene divinylbenzene copolymer
12
4.6 × 10
3mM HNO3 aq.
Shipping Solvent
For transition metal ions
Product
Code
Product
Name
F6995250
IC T-521
F6700412
IC T-G
Particle Column Size
Size
(mm)
(µm)
I.D. x Length
Shipping Solvent
Housing Material : PEEK
Line filters for IC
Product
Code
Product Name
F8500630
IC FL-1
F8500640
IC FL-1 filter
F8500650
IC FL-2
F8500660
IC FL-2 filter
Application
Contents
For general purposes
One holder and one filter
Replacement filter for IC FL-1
5 filters
Non-metal type
One holder and one filter
Replacement filter for IC FL-2
4 filters
24
*Contact Shodex or our distributors near you for customized columns.
YS-50
YK-421
10μL inj.
Sample :
1. Li+
2. Na+
3. NH4+
4. K+
5. Mg2+
6. Ca2+
2
3
1
4
2mg/L
10mg/L
10mg/L
20mg/L
10mg/L
20mg/L
YS-50 is a high performance type
column offers twice increased
theoretical plate number than YK-421
does. In particular, the peak shape is
improved. The quantitative performance
for NH4+ in a sample containing high
concentration Na+ is also enhanced.
15μL inj.
2
Standard Analysis
Columns
Standard cations (YS-50 and YK-421)
1
5
3
6
6
4
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
15
20 min
0
Shodex IC YS-50
4mM Methanesulfonic acid aq.
1.0mL/min
Non-suppressed conductivity
40˚C
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Effects of crown ether in eluent
2
3
15
2.1
TP
YS-50
YK-421
Mg2+
6,900
3,000
Ca2+
6,600
3,000
20 min
Shodex IC YK-421
5mM Tartaric acid + 1mM Dipicolinic acid + 1.5g/L Boric acid aq.
1.0mL/min
Non-suppressed conductivity
40˚C
5
6
4
Sample : 10μL
1. Li+
2mg/L
2. Na+
10mg/L
3. NH4+ 10mg/L
4. K+
20mg/L
5. Mg2+ 10mg/L
6. Ca2+ 20mg/L
1 2
3
Sample : 10μL
1. Li+
2. Na+
3. NH4+
4. K+
5. Mg2+
6. Ca2+
2mg/L
10mg/L
10mg/L
20mg/L
10mg/L
20mg/L
7. Ethylenediamine 50mg/L
5
6
6mM
2
4
1
3
4
18-Crown 6-ether conc.
YK- 421
2.5
Simultaneous analysis for cations and ethylenediamine
Crown ether forms complex with cations. The elution of
cations (particularly K+) can be well controlled by the
difference in complex formation rate.
1
10
YS-50
5
6
Columns for Ion Chromatography (Cation Analysis)
0
5
Resolution
factor of
Na+and NH4+
3mM
1
2
7
3
5
4
6
1mM
1
2
3
4
5
6
0
5
10
15
20
25
30
0mM
0
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
5
10
15
20 min
35
min
Column
Eluent
: Shodex IC YS-50
: 4mM Nitric acid + 1.5mM 18-Crown 6-ether aq.
/CH3CN=90/10
Flow rate
: 1.0mL/min
Detector
: Non-suppressed conductivity
Column temp. : 40˚C
Shodex IC YS-50
4mM Methanesulfonic acid + 18-Crown 6-ether aq.
1.0mL/min
Non-suppressed conductivity
40˚C
Alkylamines
Alkaline earth metal ions
Sample : 50μL
1. NH4+
2. Methylamine
3. Dimethylamine
4. Trimethylamine
5. Ethylamine
6. Propylamine
7. Butylamine
1
min
2
5mg/L
5mg/L
5mg/L
20mg/L
10mg/L
10mg/L
10mg/L
3
4
Cu2+
Zn2+
N i 2+
Co2+
2+
Mg
Fe2+, Fe3+
Mn2+, Pb3+ 2+
Cd
1
4
Ca2+
3
2
2
Sr 2+
3
4
Sample : 10μL
1. Ni2+
50mg/L
2. Mg2+ 20mg/L
3. Mn2+ 50mg/L
4. Ca2+ 50mg/L
5
2
6
5
6
7
7
0
1
2
3
4
5 min
B a 2+
0
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
5
Shodex IC YK-421
4mM H3PO4 aq./CH3CN=90/10
1.0mL/min
Non-suppressed conductivity
25˚C
10 min
8
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Shodex IC Y-521
4mM Tartaric acid + 2mM Ethylenediamine aq.
1.0mL/min
Non-suppressed conductivity
40˚C
25
Standard Analysis
Columns
Column Selection for Size Exclusion
Chromatography (SEC)
Application
Solvent
Biological macromolecules
(proteins, peptides, nucleic acids, etc.)
Column Selection for Size Exclusion Chromatography (SEC)
Aqueous SEC
(GFC)
Page
KW-800 series
28
High performance
(solvent-saving)
Buffer etc.
KW400 series
Biological macromolecules
(high MW range)
Buffer etc.
SB-800 HQ series
30
Water-soluble polymers
(polyacrylamide, polyethylenimine, etc.)
Polysaccharides
H2O, Buffer
Aqueous
solution etc.
SB-800 HQ series
30
H2O,
Aqueous
solution
KS-800 series
16
Oligosaccharide, polysaccharides
GS-HQ series
32
THF
KF-800 series
General polymers
Organic SEC
(GPC)
Buffer etc.
Column
Polar polymers
(polyimides, polyvinylpyrrolidones etc.)
Analysis at high temperature
(polyethylene, polypropylene etc.)
Engineering resin analysis at room
temperature
[polyamide (Nylon), polyethylene
terephthalate (PET) etc.]
28
36
THF
KF-600 series
Rapid analysis
(solvent-saving)
THF
KF-400HQ series
High performance
(solvent-saving)
42
THF
LF series
High linearity of
calibration curve
44
Chloroform
K-800 series
38
DMF
KD-800 series
40
DMF
SB-800 HQ series
30
ODCB etc.
HT-800 series
46
ODCB etc.
UT-800 series
46
ODCB etc.
AT-806MS
46
HFIP
HFIP-800 series
48
HFIP
HFIP-600 series
Rapid analysis
(solvent-saving)
HFIP
LF series
High linearity of
calibration curve
Aqueous/Organic
SEC
42
48
44
34
GF-HQ series
Guideline for column solvent usability
Polarity of solvent
High
Low
H2O
Methanol
DMSO
Acetonitrile
DMF
THF
Chloroform
KW-800 series, KW400 series
SB-800 HQ series
GS-HQ series
GF-HQ series
KD series
KF, K, LF series
26
* See page 50 for the solvent replaceability of organic solvent SEC (GPC) packed columns.
Toluene
Size exclusion chromatography analysis of polar polymers can be influenced by unexpected interactions in the column.
These interactions may change elution patterns and results in an invalid molecular weight calculation.
It is important to reduce them in order to obtain the accurate molecular weight distribution.
Interfering interactions likely to be observed
Standard Analysis
Columns
Precautions for Polar Polymer Analysis
Interactions between the analyte and the packing materials
Elution will be
retarded
log MW
Exclusion limit
molecular weight
Elution will be
accelerated
(B)
(A)
Exclusion
limit volume
Permeation
limit volume
Interaction within and between the analyte
Ionic repulsion effects observed within the multivalent
macromolecules causes structure expansion
This accelerates the analyte elution, and thus results in
over estimation of its molecular weight (Figure A).
Association between the molecules
Associated molecule detected as a larger molecule
(Figure A).
(C)
(D)
Precautions for Polar Polymer Analysis
Hydrophobic interaction
The analyte is adsorbed into the packing material.
This delays the analyte elution, and thus results in
underestimation of its molecular weight (Figure B, D).
Ionic interaction
(1) Ion Exclusion
The analyte is repelled from the packing material.
This accelerates the analyte elution, and thus results
in overestimation of its molecular weight (Figure A, C).
(2) Ion Exchange
The analyte is adsorbed onto the packing material.
This delays the analyte elution, and thus results in
underestimation of its molecular weight (Figure B, D).
Interactions between the analyte and the solvent
The multivalent ion of the solvent works as a bridge to
bind ionic molecules (analyte).
Methods to reduce interactions
Aqueous SEC (GFC)
Ionic Interaction
Add salt
Hydrophobic interaction
Increase dissociation of the analyte
Cationic polymer
Lower the pH
Anionic polymer
Higher the pH
Lower the eluent polarity
(Example) Add acetonitrile or methanol
Organic solvent SEC (GPC)
Ionic Interaction
Add salt
(Example) Add LiBr to DMF
Add CF3COONa to HFIP
Hydrophobic interaction
Lower the polarity of the eluent
(Example) Change the eluent from DMF to THF
Hydrophillic interaction
Increase the polarity of the eluent
(Example) Change the eluent from THF to DMF
27
Standard Analysis
Columns
Aqueous SEC (GFC) Columns : Silica-based
Features
KW-800
Silica-based packed columns for aqueous SEC (GFC) analysis
Suitable for the analysis of proteins and enzymes
Note
book
KW400
No.7
Aqueous SEC (GFC) Columns : Silica-based
Preparative
Columns
p.81
Semi-micro
Micro Columns
p.73
Reduced particle size of the packing material enhances column performance
Three- or fourfold higher sensitivity than KW-800 series
KW405-4F is applicable to samples with a molecular weight above 1,000,000
Note
book
No.5, 7
News
No.31
Standard columns
Particle
Size
(µm)
Exclusion Limit
Shipping
Solvent
400
8.0 × 300
H2O
5
1,000
8.0 × 300
H2O
7
1,500
8.0 × 300
H2O
7
−
6.0 × 50
H2O
Product Name
Plate Number
(TP/column)
F6989000
PROTEIN KW-802.5
≥ 21,000
60,000
150,000
5
F6989103
PROTEIN KW-803
≥ 21,000
170,000
700,000
F6989104
PROTEIN KW-804
≥ 16,000
500,000
(1,000,000)*
F6700131
PROTEIN KW-G
(Pullulan)
(guard column)
(Protein)
−
−
Maximum
Pore Size
(Å)
Column Size
(mm)
I.D. x Length
Product Code
Base Material : Silica
Usable pH range : pH3.0-7.5
( )* Estimated value
High performance semi-micro columns
Product Code
Product Name
Plate Number
(TP/column)
Use of the KW400 series with semi-micro type devices is recommended.
Particle
Size
(µm)
Exclusion Limit
(Pullulan)
(Protein)
Maximum
Pore Size
(Å)
Column Size
(mm)
I.D. x Length
Shipping
Solvent
F6989201
KW402.5-4F
≥ 35,000
60,000
150,000
3
400
4.6 × 300
H2O
F6989202
KW403-4F
≥ 35,000
150,000
600,000
3
800
4.6 × 300
H2O
F6989203
KW404-4F
≥ 25,000
500,000
(1,000,000)*
5
1,500
4.6 × 300
H2O
F6989204
KW405-4F
≥ 25,000
1,300,000
(20,000,000)*
5
2,000
4.6 × 300
H2O
F6700132
KW400G-4A
(guard column)
−
−
5
−
4.6 × 10
H2O
Base Material : Silica
Usable pH range : pH3.0-7.5
( )* Estimated value
Molecular weight range with protein (eluent : phosphate buffer)
Molecular weight (Protein)
102
103
104
105
106
107
KW-802.5, 402.5-4F
KW-803, 403-4F
KW-804, 404-4F
KW405-4F
Calibration
28
Standards
See page 51 for
Calibration Standards
*Contact Shodex or our distributors near you for customized columns.
108
Recovery (%)
107
Protein
107
KW402.5-4F KW403-4F
KW404-4F
KW405-4F
10
6
Molecular weight (Protein)
Molecular weight (Protein)
6
105
104
KW402.5-4F
KW403-4F
102
1.5
KW-804
105
104
KW-802.5
2.5
3.0
3.5
4.0
5
4.5
6
7
8
9
10
11
12
: Shodex KW400-4F series, PROTEIN KW-800 series
: 50mM Sodium phosphate buffer + 0.3M NaCl(pH7.0)
: (KW400) 0.33mL/min
(KW-800) 1.0mL/min
Detector
: (KW400) UV(280nm) (small cell volume)
(KW-800) UV(280nm) (conventional type)
Column temp. : 25˚C
Comparison of KW402.5-4F and KW-802.5
97
Myoglobin
90
89
Cytochrome c
92
92
Lysozyme
87
98
α-Chymotrypsinogen A
95
94
: Shodex KW402.5-4F, KW403-4F
: 50mM Sodium phosphate buffer
+ 0.3M NaCl(pH7.0)
Flow rate
: 0.33mL/min
Detector
: UV(280nm) (small cell volume)
Column temp. : 25˚C
Lectins
Sample : 5μL
1. Lectin from Soybean
2. Lectin from Arachis hypogaea
3. Lectin from Canavalia ensiformis (Con A)
4. Lectin from Lens culinaris (LCA)
5. Lectin from Triticum vulgaris (WGA)
Sample : Whey, 5μL
5
0.6mg/mL
1.1mg/mL
0.9mg/mL
0.7mg/mL
0.8mg/mL
5
3
N=17,000
2
4
1
2
1
KW402.5-4F
96
89
Column
Eluent
Whey in yogurt
KW400 series are high performance type
semi-micro columns, and offer approximately 1.5
times larger theoretical plate number and 3 to 4
times higher detection sensitivity (peak height) than
KW-800 series columns.
3
89
Ovalbumin
13
Column
Eluent
Flow rate
0.2mg/mL
0.8mg/mL
0.8mg/mL
0.56mg/mL
0.04mg/mL
Bovine serum albumin
Elution volume (mL)
Elution volume (mL)
Sample : 10μL
1. Blue dextran 2000
2. γ-Globulin
3. Ovalbumin
4. Myogrobin
5. Uridine
96
KW-803
103
102
2.0
98
Aqueous SEC (GFC) Columns : Silica-based
103
10
γ - Globulin
Standard Analysis
Columns
Recovery rate of proteins
Calibration curves for KW400 series and KW-800 series using protein
4
5
KW-802.5
1 2
0
3
5
N=8,200
4
10
20
15
min
: Shodex KW402.5-4F
PROTEIN KW-802.5
Eluent
: 50mM Sodium phosphate buffer
+ 0.3M NaCl(pH7.0)
Flow rate
: (A) 0.33mL/min, (B) 1.0mL/min
Detector
: UV(280nm) (small cell volume)
Column temp. : 25˚C
30
40
50 min
0
5
10 min
Column
Column
Eluent
: Shodex KW402.5-4F + KW403-4F
: 50mM Sodium phosphate buffer
+ 0.3M NaCl(pH7.0)
Flow rate
: 0.20mL/min
Detector
: UV(280nm) (small cell volume)
Column temp. : 30˚C
Comparison of various GFC columns for separation of
standard proteins
Sample :
1. Thyroglobulin (bovine)
2. γ-Globulin (bovine)
3. Ovalbumin (chicken)
4. Myoglobin (horse)
5. Cyanocobalamin
1
4
2
5
3
Molecular weight (Protein)
5
2
SB-803 HQ
Sample : 0.1% each
1. Fibrinogen
50μL
2. α2 -Macroglobulin 50μL
3. IgG
50μL
4. Transferrin
50μL
5. Plasminogen
50μL
6. Albumin
100μL
7. Antitrypsin
100μL
8. Hemoglobin
100μL
1
106
3
: Shodex KW402.5-4F
: 50mM Sodium phosphate buffer
+ 0.3M NaCl(pH7.0)
Flow rate
: 0.33mL/min
Detector
: UV(220nm) (small cell volume)
Column temp. : 30˚C
Proteins in human blood serum
Three aqueous SEC columns (SB-803 HQ, GF-510 HQ,
and KW-803) were compared for their separation
performances. KW-803, silica-based column, showed
the best separation performance for the analysis of
protein standards.
4
Column
Eluent
2
KW-803
GF-510 HQ
SB-803 HQ
3
4
105
5
6
104
1
GF-510 HQ
11
13
15
17
19
21 min
7
5
: Shodex OHpak SB-803 HQ
Asahipak GF-510 HQ
PROTEIN KW-803
Eluent
: 0.2M Phosphate buffer(pH6.9)
Flow rate
: 0.5mL/min
Detector
: UV(280nm)
Column temp. : 30˚C
2
3
1
KW-803
0
5
10
15
20
8
Column
4
25
30
35 min
0
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
15 min
Shodex PROTEIN KW-803
50mM Phosphate buffer + 0.3M NaCl(pH7.0)
1.0mL/min
UV(280nm)
Room temp.
29
Standard Analysis
Columns
Aqueous SEC (GFC) Columns : Polymer-based
Features
SB-800 HQ
Aqueous SEC (GFC) Columns : Polymer-based
Polymer-based packed columns for aqueous SEC (GFC) analysis
Supports a wide range of molecular weight sample analysis
The eluent can be replaced with DMF (except SB-802 HQ and SB-807 HQ),
enabling the analysis of polar polymers
Method using SB-804 HQ or SB-805 HQ for gelatin’s mean molecular weight
determination is comparable with PAGI method (Ver. 10, Japan)
News
SB-807 HQ
Semi-micro
Micro Columns
p.74
Preparative
Columns
p.81
No.29
Column for the analysis of water-soluble ultra high molecular weight polymers
Large particle size gel is packed to prevent shear degradation of polymers
Standard columns
Product Code
Exclusion Limit
(Pullulan)
Plate Number
(TP/column)
Product Name
Particle Size
(µm)
Maximum
Pore Size
(Å)
Column Size
(mm)
I.D. x Length
Shipping Solvent
F6429100
OHpak SB-802 HQ
≥ 12,000
4,000
8
100
8.0 × 300
0.02% NaN3 aq.
F6429101
OHpak SB-802.5 HQ
≥ 16,000
10,000
6
200
8.0 × 300
0.02% NaN3 aq.
F6429102
OHpak SB-803 HQ
≥ 16,000
100,000
6
800
8.0 × 300
0.02% NaN3 aq.
F6429103
OHpak SB-804 HQ
≥ 16,000
(1,000,000)
10
2,000
8.0 × 300
0.02% NaN3 aq.
F6429104
OHpak SB-805 HQ
≥ 12,000
(4,000,000)*
13
7,000
8.0 × 300
0.02% NaN3 aq.
F6429105
OHpak SB-806 HQ
≥ 12,000
(20,000,000)*
13
15,000
8.0 × 300
0.02% NaN3 aq.
F6429106
OHpak SB-806M HQ
≥ 12,000
(20,000,000)*
13
15,000
8.0 × 300
0.02% NaN3 aq.
F6709430
OHpak SB-G
(guard column)
−
10
−
6.0 × 50
0.02% NaN3 aq.
SB-806M HQ is a mixed-gel column capable of analyzing
samples over a wide range of molecular weight distribution.
Base Material : Polyhydroxymethacrylate
Usable pH range : pH3-10
( )* Estimated value
For water-soluble ultra high molecular weight polymers
Product Code
Product Name
F6429108
OHpak SB-807 HQ
F6709431
OHpak SB-807G
Plate Number
(TP/column)
Exclusion Limit
(Pullulan)
Particle Size
(µm)
Maximum
Pore Size
(Å)
Column Size
(mm)
I.D. x Length
Shipping Solvent
≥ 1,500
(500,000,000)*
35
30,000
8.0 × 300
H2O
(guard column)
−
35
−
8.0 × 50
H2O
Base Material : Polyhydroxymethacrylate
Usable pH range : pH3-10
( )* Estimated value
Usable concentration of organic solvents
The maximum usable concentration (%)
Methanol Acetonitrile
DMF
Product Name
SB-802 HQ
0
0
0
SB-802.5 HQ, SB-803 HQ
100
75
100
SB-804 HQ~SB-806M HQ
75
75
100
SB-G
75
75
100
SB-807 HQ, SB-807G
30
30
(Note)
The maximum solvent tolerance of
preparative type SB-800 HQ, SB-2000
series, is 50% of methanol, acetonitrile,
and DMF (SB-2002 is not tolerant of
organic solvents, similar to SB-802 HQ).
Calibration
Standards
0
Molecular weight range with pullulan
(eluent : ultrapure water)
Molecular weight range with PEG/PEO
(eluent : DMF)
Molecular weight (Pullulan)
10
102
103
104
105
106
See page 51 for
Calibration Standards
Molecular weight (PEG/PEO)
107
108
109
SB-802 HQ
10
102
103
104
105
106
SB-802.5 HQ
SB-802.5 HQ
SB-803 HQ
SB-803 HQ
SB-804 HQ
SB-804 HQ
SB-805 HQ
SB-805 HQ
SB-806 HQ
SB-807 HQ
SB-806M HQ
30
*Contact Shodex or our distributors near you for customized columns.
SB-806 HQ
SB-806M HQ
107
108
109
Calibration curves for SB-800 HQ series
using PEG / PEO (eluent : DMF)
Dextran sulfate
Sample : Dextran sulfate 0.1%, 200μL
107
SB
107
-8
SB
106
HQ
106
-8
-8
02
Q
HQ
SB
103
HQ
6M
HQ
HQ
HQ
02
HQ
-8
104
04
SB
103
H
6M
-80
SB
.5
H
Q
Q
02
H
-8
3
SB
104
05
03
-8
SB
04
-8
105
-80
SB
-8
-8
SB
SB
105
SB
6H
-80
SB
HQ
MW : 5,000
.5
HQ
Q
102
102
4
5
6
7
8
9
10
11
MW : 500,000
4
12
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
6
7
8
9
10
11
12
5
10
15
20
25
Elution volume (mL)
Elution volume (mL)
Shodex OHpak SB-800 HQ series
H2O
1.0mL/min
RI
Room temp.
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Shodex OHpak SB-800 HQ series
20mM LiBr in DMF
0.8mL/min
RI
40˚C
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
30
min
Shodex OHpak SB-806M HQ x 2
0.1M NaCl aq.
1.0mL/min
RI
40˚C
Effects of sodium nitrate concentration in eluent
on the analysis of polyallylamine
SEC analysis of humic substance
For the analysis of cationic polymers, such as polyallylamine,
undesired adsorption of the polymer is observed when low
(0.02M) sodium nitrate eluent was used. By using higher
concentration (>0.1M) salt, it suppresses the sample adsorption
and enables to obtain accurate chromatograms.
Reference materials (from the Japanese
Humic Substances Society) for humic
acid and fulvic acid, prepared from
brown forest soil (Dando) and kuroboku
soil (Inogashira), were analyzed using
SEC columns.
Calibration curve of Sodium polystyrene sulfonate
Sample : Humic substances, 30μL
Humic acid 0.02mg/mL
Fulvic acid 0.02mg/mL
HUMIC ACID
(Dando soil)
Sample : Polyallylamine 0.2%, 100μL
0.1 M
Aqueous SEC (GFC) Columns : Polymer-based
Molecular weight (PEG/PEO)
05
0
-8
SB
Molecular weight (Pullulan)
06
Standard Analysis
Columns
Calibration curves for SB-800 HQ series
using pullulan (eluent : H2O)
0.02 M
0.2 M
HUMIC ACID
(Inogashira soil)
FULVIC ACID
(Dando soil)
0.05 M
FULVIC ACID
(Inogashira soil)
7
9
11
13
15
8.0
17 min
12.0
16.0
20.0
24.0
Volume (mL)
Vo
Vt
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Column
: Shodex OHpak SB-806M HQ x 2
Eluent
: 0.5M Acetic acid + NaNO3 aq.
Flow rate
: 1.0mL/min
Detector
: RI
Column temp. : 40˚C
Shodex OHpak SB-G + SB-805 HQ
10mM NaH2PO4 + 10mM Na2HPO4(pH7.0) + 25% CH3CN
0.8mL/min
Data courtesy of Associate Professor Nobuhide Fujitake,
UV(260nm)
Student Affairs Section, Graduate School of Agricultural Science,
40˚C
Kobe University
Carboxymethylcellulose
Polycarbonate
Polyacrylamide
Sample : Polycarbonate
Sample : 200μL
Carboxymethylcellulose (Medium viscosity) 0.1%
Sample : Polyacrylamide, 100μL
SB-806 HQ
CH3
O
C
CH3
O C
O
n
1.0x108
Molar Mass(g/mol)
SB-807 HQ
1.0x107
1.0x106
1.0x105
4.0
Mw: 5,500,000
6.0
8.0
10.0
12.0
14.0
Volume(mL)
0
2
4
6
8
10 12 14 16 18 20 22 24 26 28 30
min
8
Column
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Shodex OHpak SB-806M HQ x 2
0.1M NaCl aq.
1.0mL/min
RI
40˚C
12
16
20 min
: Shodex OHpak
SB-805 HQ + SB-802.5 HQ
Eluent
: 5mM LiBr in DMF
Flow rate
: 1.0mL/min
Detector
: RI
Column temp. : 40˚C
Column
Eluent
Flow rate
Detector
:
:
:
:
Shodex OHpak SB-807 HQ, SB-806 HQ
0.2M NaCl aq.
0.5mL/min
RI
MALS(Multi angle laser light scattering)
Column temp. : 30˚C
31
Standard Analysis
Columns
Multimode Columns
Features
GS-HQ
Multimode Columns
SEC is the main separation mode
According to the eluent selected, the column adds multimode features of
reversed phase, HILIC, and ion exchange modes to SEC
Suitable for the separation of peptides or nucleic acids with similar molecular
weights
Suitable for desalting samples or substituting buffer in protein analysis
Note
book
GS-620 7G-P
No.3
Semi-micro
Micro Columns
p.75
Preparative
Columns
p.82
Columns to determine molecular weight distribution of gelatin compliant with
PAGI method (Ver. 10, Japan)
Standard columns
Column Size
(mm)
I.D. x Length
Shipping Solvent
150
7.5 × 300
H2O/CH3OH=70/30
6
400
7.5 × 300
H2O/CH3OH=70/30
300,000
7
2,000
7.5 × 300
H2O/CH3OH=70/30
≥ 18,000
(2,000,000)**
7
7,000
7.5 × 300
H2O/CH3OH=70/30
(guard column)
−
9
−
7.5 × 50
H2O/CH3OH=70/30
Exclusion Limit
(Pullulan)
Particle
Size
(µm)
Product Code
Product Name
Plate Number
(TP/column)
F7600005
Asahipak GS-220 HQ
≥ 19,000
3,000*
6
F7600006
Asahipak GS-320 HQ
≥ 19,000
40,000
F7600007
Asahipak GS-520 HQ
≥ 18,000
F7600008
Asahipak GS-620 HQ
F6710019
Asahipak GS-2G 7B
Molecular weight range with pullulan (eluent : ultrapure water)
Maximum
Pore Size
(Å)
Base Material : Polyvinyl alcohol
Usable pH range : pH2-12
(GS-220 HQ : pH2-9)
Usable concentration of methanol is up to 100%
(GS-220 HQ : up to 30%)
Usable concentration of acetonitrile is up to 50%
* PEG equivalent
( )** Estimated value
Molecular weight (Pullulan)
102
10
103
104
105
106
107
GS-220 HQ
GS-320 HQ
GS-520 HQ
GS-620 HQ
For determination of gelatin molecular weight distribution
Product Code
Product Name
Application
Column Size
(mm)
I.D. x Length
Shipping Solvent
F7600023
Asahipak GS-620 7G-P
Gelatin for photo film
7.5 × 500
H2O/CH3OH=70/30
F6710019
Asahipak GS-2G 7B
(guard column)
7.5 × 50
H2O/CH3OH=70/30
Calibration
32
Standards
See page 51 for
Calibration Standards
*Contact Shodex or our distributors near you for customized columns.
Peptides
GS-HQ columns can be used not only for SEC (GFC) in an
aqueous system, but also for multimodal analysis where
hydrophobic interaction and ionic interaction are used
together as separation criteria, under certain conditions of
the eluent. This results in unprecedented separation analysis.
GS-320 HQ columns are excellent in the performance for
separating hydrophilic peptides, in particular, acidic or basic
peptides, from each other.
Pullulan
PEG
106
GS
Molecular weight
-6
10
5
10
4
GS
-3
20
GS
20
HQ
-5
20
HQ
103
GS
pl
Σf
347
3.12
0.39
-22
Arg-Asp
289
6.75
0.68
Gly-His-Lys
340
9.95
0H
Q
102
0.29
Arg-Pro-Lys-Pro
497
11.44
3.24
0.025%
0.05%
0.025%
0.025%
3
2
Multimode Columns
MW
Glu-Ala-Glu
HQ
Sample : 20μL
1. Glu-Ala-Glu
2. Arg-Asp
3. Gly-His-Lys
4. Arg-Pro-Lys-Pro
4
Σ f : Hydrophobic parameter
p l : Isoelectric point
4
5
6
7
8
9
10
11
12
1
Column
Eluent
: Shodex Asahipak GS-320 HQ
: 30mM Ammonium acetate buffer
(pH6.7)
Flow rate
: 0.5mL/min
Detector
: UV(220nm)
Column temp. : 30˚C
Elution volume (mL)
Column
: Shodex Asahipak GS-HQ series
Eluent
: H2O
Flow rate
: 0.6mL/min
Detector
: RI
Column temp. : 30˚C
0
Low molecular weight water-soluble dietary fiber
5
Standard Analysis
Columns
Calibration curves for GS-HQ series using
pullulan and PEG
10
15
20
25
30
min
Analysis of purine bases in beer
By using GS-220 HQ, monosaccharides, disaccharides,
and sugar alcohols elute after indigestible component
fraction (indicated by an arrow on the chromatogram).
This separation makes the method preferable for the
quantification of low molecular weight water-soluble
dietary fiber.
Purine in foods is analyzed as purine base after a step of sample preparation; homogenization,
freeze drying, hydolyzation with 70% perchloric acid, and neutralization. Examples below show
the analysis of purin in regular beer and beer treated with guanase (an enzyme that degrades
guanine to xanthine). The following data indicates that guanine was decreased and xanthine was
increased by guanase.
Normal beer
Guanase treated beer
guanine
xanthine
xanthine
15
10
20
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
25
30
35
40
45
Shodex Asahipak GS-220 HQ x 2
H2O
0.5mL/min
RI
60˚C
“Umami”
5
1
4
30
40
50 min
10
15
20
0
10
Shodex Asahipak GS-320 HQ
150mM Sodium phosphate buffer(pH2.5)
0.6mL/min
UV(260nm)
35˚C
20
30
40
50 min
Data provided by Kiyoko Kaneko Ph.D.,
Faculty of Pharmaceutical Sciences,
Teikyo University
Gelatin analysis with PAGI method
Sample : 100μL
Gelatin(Koepff 16922) 0.2%
Sample : 100μL
Lignosulfonic acid sodium salt 0.1%
8
7
6
Column
Eluent
20
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Sample : 50μg/mL, 20μL
1. IMP
2. GMP
3. AMP
4. Inosine
5. Hypoxanthine
6. Guanosine
7. Guanine
8. Adenosine
9. Adenine
2
5
10
Lignosulfonic acid
3
0
0
50
min
9
25
30
35
40
min
: Shodex Asahipak GS-320 HQ
: 10mM NaH2PO4 aq./10mM Na2HPO4 aq.
=1000/31
Flow rate
: 1.0mL/min
Detector
: UV(260nm)
Column temp. : 40˚C
0
5
10
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
15
20
25
30
35
40
min
Shodex Asahipak GS-520 HQ x 2
20mM Na2HPO4 aq.
0.6mL/min
UV(254nm)
40˚C
5
10
15
20
25
30
35
40
min
Column
Eluent
: Shodex Asahipak GS-620 7G-P x 2
: 0.1M KH2PO4 aq./0.1M Na2HPO4 aq.
=50/50
Flow rate
: 1.0mL/min
Detector
: UV(230nm)
Column temp. : 50˚C
33
Standard Analysis
Columns
Aqueous/Organic SEC Columns
Features
GF-HQ
Polymer-based SEC columns exhibit high solvent durability
Supports both aqueous and organic solvents
No.2
News
NEW GF-210 HQ
Semi-micro
Micro Columns
p.76
Preparative
Columns
p.82
Column for low molecular weight substance analysis with exclusion limit
of 9,000 (pullulan)
Aqueous/Organic SEC Columns
GF-310
Semi-micro columns for Asahipak GF-310 HQ
Effective for drug analysis in biological samples by using column switching
method to remove proteins
Useful for LC/MS analysis
Suitable for the analysis of surfactants
No.2
News
Standard columns
Particle
Size
(µm)
Plate Number Exclusion Limit
(TP/column)
(Pullulan)
Product Code
Product Name
F7600000
NEW Asahipak GF-210 HQ
≥ 19,000
F7600001
Asahipak GF-310 HQ
F7600002
Asahipak GF-510 HQ
F7600003
F7600004
F6710018
Maximum
Pore Size
(Å)
Column Size
(mm)
I.D. x Length
Shipping Solvent
180
7.5 × 300
H2O
9,000
5
≥ 19,000
40,000
5
400
7.5 × 300
H2O/CH3OH=70/30
≥ 19,000
300,000
5
2,000
7.5 × 300
H2O/CH3OH=70/30
Asahipak GF-710 HQ
≥ 11,000
(10,000,000)*
9
10,000
7.5 × 300
H2O/CH3OH=70/30
Asahipak GF-7M HQ
≥ 13,000
(10,000,000)*
9
10,000
7.5 × 300
H2O/CH3OH=70/30
Asahipak GF-1G 7B
(guard column)
−
9
−
7.5 × 50
H2O/CH3OH=70/30
GF-7M HQ is a mixed-gel column capable of analyzing samples over a wide range of molecular weight.
Base Material : Polyvinyl alcohol
Usable pH range : pH2-9
( )* Estimated value
Usable solvents
GF-210 HQ
Solvent
GF-310 HQ
GF-510 HQ
GF-710 HQ
GF-7M HQ
GF-310 HQ
GF-510 HQ
GF-710 HQ
GF-7M HQ
GF-210 HQ
Solvent
Water
(0-0.5M sodium concentration)
DMF
Methanol
Acetone
Ethanol
Chloroform
Acetonitrile
Ethylacetate
THF
DMSO
(Note)
The usable solvents for preparative
columns of GF-710 HQ, GS-710 20F
and 20G, are water and methanol.
GSM-700 20F or 20G is recommended
when other solvents are required.
0~50%
* When replacing acetonitrile, ethyl acetate or chloroform with water, replace with methanol first and then replace with water.
* When replacing water with ethyl acetate or chloroform, replace with methanol first and then replace with the required eluent condition.
Molecular weight range with pullulan
(eluent : ultrapure water)
Molecular weight range with PEG/PEO
(eluent : DMF)
Molecular weight (Pullulan)
10
102
103
104
105
Molecular weight (PEG/PEO)
106
107
108
10
GF-210 HQ
102
103
104
105
106
107
108
GF-210 HQ
GF-310 HQ
GF-310 HQ
GF-510 HQ
GF-510 HQ
GF-710 HQ
GF-710 HQ
GF-7M HQ
GF-7M HQ
Semi-micro columns
Plate Number
(TP/column)
Particle
Size
(µm)
Maximum
Pore Size
(Å)
Column Size
(mm)
I.D. x Length
Shipping
Solvent
MSpak GF-310 4B
≥ 3,000
5
400
4.6 × 50
H2O
MSpak GF-310 4D
≥ 10,000
5
400
4.6 × 150
H2O
F7600024
MSpak GF-310 4E
≥ 16,000
5
400
4.6 × 250
H2O
F7600120
MSpak GF-310 2D
≥ 5,500
5
400
2.0 × 150
H2O
Product Code
Product Name
F7600100
F7600110
Calibration
34
*Contact Shodex or our distributors near you for customized columns.
Standards
See page 51 for
Calibration Standards
Calibration curves for GF-HQ series using
PEG/PEO
Polyacrylonitrile
Sample : Polyacrylonitrile 0.1% (w/v), 100μL
107
107
GF
10
HQ
HQ
G
10
HQ
HQ
3
10
10
10
104
HQ
G
F-
31
G
F-
0
21
0
3
10
HQ
H
Q
102
3
4
5
6
7
8
9
10
10
11
3
4
5
Elution volume (mL)
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
6
7
8
9
10
11
Elution volume (mL)
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Shodex Asahipak GF-HQ series
H2O
0.6mL/min
RI
30˚C
Shodex Asahipak GF-HQ series
DMF
0.6mL/min
RI
40˚C
Comparison of polystyrenes separation using various solvents
5
(B) Chloroform
10
15
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
20
25
30
35
40
min
Shodex Asahipak GF-710 HQ x 2
20mM LiBr in DMF
0.6mL/min
RI
40˚C
Sodium polystyrene sulfonates
Sample : Polystyrene 1mg/mL each, 50μL
1. Mw : 1,090,000
2. Mw : 190,000
3. Mw : 37,900
4. Mw :
9,100
5. Mw :
2,980
6. Mw :
500
1
(A) THF
0
Polymers having both hydrophobic
and hydrophilic functional groups
may exhibit hydrophobic interactions
with packing materials.
When analyzing such polymers, the
hydrophobic interaction can be
suppressed by adding organic
solvents to the eluent.
MW:104,000
(C) DMF
Aqueous/Organic SEC Columns
102
-7
10
HQ
2
F-
0
GF
M
G
31
HQ
F-
5
-7
GF
M
-7
GF
Molecular weight (Pullulan)
0
5
10
Q
51
Molecular weight (PEG/PEO)
F-
104
106
-7
G
0H
-51
GF
106
Standard Analysis
Columns
Calibration curves for GF-HQ series using
pullulan
1
0
1
2
10
20
30 min
Sample : 1mg/mL each, 50μL
Sodium polystylene sulfonate
3
2
4
3
2
3
4
5
0
6
20 min
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
5
0
6
CH2
4
5
20 min
0
6
5
1
GF-210 HQ x 2
1~3
6
SO3Na
20 min
n
Column
MW:8,000
0
GF-210 HQ demonstrates an improved separation of low molecular
substances. Below example shows that the peaks obtained by GF-210 HQ are
separated with deeper notches compared to peaks obtained by GF-310 HQ.
GF-210 HQ is capable of separating oligosaccharides (trisaccharides to
hexasaccharides) while those oligosaccharides were eluted all together when
analyzed by GF-310 HQ.
4
10
: Shodex Asahipak
GF-510 HQ
: 50mM LiCl aq./CH3CN
=60/40
Flow rate
: 0.6mL/min
Detector
: UV(254nm)
Column temp. : 30˚C
Eluent
Comparison of two GF columns for the separation performance
of maltooligosaccharides
3
0
20 min
Shodex Asahipak GF-510 HQ + GF-310 HQ
(A); THF, (B); Chloroform, (C); DMF
0.5mL/min
(A),(B) UV(254nm), (C) UV(270nm)
30˚C
2
CH
MW:38,000
Sample : 0.1% each, 20μL
1. Maltohexaose
2. Maltopentaose
3. Maltotetraose
4. Maltotriose
5. Maltose
6. Glucose
10
20
30 min
Comparison of two GF columns for the separation performance
of triglycerides
Sample : 50μL
1. Tristearin 0.1%
2. Tricaprin 0.2%
3. Tricaproin 0.2%
4. Tributyrin 1.0%
2
4
3
1
GF-210 HQ
2
4
3
4
1
5
GF-310 HQ
6
GF-310 HQ x 2
15
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
20
25
30
Shodex Asahipak GF-210 HQ x 2, GF-310 HQ x 2
H2O
0.6mL/min
RI
50˚C
35 min
0
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
5
10
Shodex Asahipak GF-210 HQ, GF-310 HQ
Chloroform
0.6mL/min
RI
50˚C
15 min
35
Standard Analysis
Columns
Organic SEC (GPC) Columns (General Analysis) :
THF
Features
KF-800
Standard organic solvent SEC (GPC) column
Supports a wide range of applications from low to high molecular weight
compounds
Preparative
Columns
Organic SEC (GPC) Columns (General Analysis) : THF
Standard columns
KF-800 series : Shipping solvent Tetrahydrofuran (THF)
Product Code
Product Name
Plate Number
(TP/column)
Exclusion Limit
(Polystyrene)
Particle Size
(µm)
Maximum
Pore Size
(Å)
Column Size
(mm)
I.D. x Length
F6028010
GPC KF-801
≥ 18,000
1,500
6
50
8.0 × 300
F6028020
GPC KF-802
≥ 18,000
5,000
6
150
8.0 × 300
F6028025
GPC KF-802.5
≥ 18,000
20,000
6
300
8.0 × 300
F6028030
GPC KF-803
≥ 18,000
70,000
6
500
8.0 × 300
F6027030
GPC KF-803L
≥ 18,000
70,000
6
500
8.0 × 300
F6028040
GPC KF-804
≥ 18,000
400,000
7
1,500
8.0 × 300
F6027040
GPC KF-804L
≥ 18,000
400,000
7
1,500
8.0 × 300
F6028050
GPC KF-805
≥ 11,000
4,000,000
10
5,000
8.0 × 300
F6027050
GPC KF-805L
≥ 11,000
4,000,000
10
5,000
8.0 × 300
F6028060
GPC KF-806
≥ 11,000
(20,000,000)*
10
10,000
8.0 × 300
F6028090
GPC KF-806M
≥ 13,000
(20,000,000)*
10
10,000
8.0 × 300
F6027060
GPC KF-806L
≥ 11,000
(20,000,000)*
10
10,000
8.0 × 300
F6028070
GPC KF-807
≥ 6,000
(200,000,000)*
18
20,000
8.0 × 300
F6027070
GPC KF-807L
≥ 6,000
(200,000,000)*
18
20,000
8.0 × 300
F6700300
GPC KF-G
(guard column)
−
8
−
4.6 × 10
solvent-peak
separation column
−
10
−
8.0 × 100
GPC KF-800D
F6709350
* See page 40 for details of the solvent-peak separation columns. The columns with ‘L’ or ‘M’ at
the end of column names are mixed-gel column capable of analyzing samples over a wide range
of molecular weight distribution.
Base Material : Styrene divinylbenzene copolymer
( )* Estimated value
* See page 50 for applicability of SEC (GPC) columns to solvent replacement.
Molecular weight range with polystyrene (eluent : THF)
Molecular weight (Polystyrene)
10
102
103
104
105
106
107
KF-801
KF-802
KF-802.5
KF-803
KF-804
KF-805
KF-806
KF-807
KF-803L
KF-804L
KF-805L
KF-806M
KF-806L
KF-807L
Calibration
36
p.82, 83
Standards
See page 51 for
Calibration Standards
*Contact Shodex or our distributors near you for customized columns.
108
109
Calibration curves for KF-800L (linear type)
series using polystyrene
108
108
107
-8
Molecular weight (Polystyrene)
KF
-80
5
KF
105
-8
04
10
-8
KF
6M
KF
4
0
-8
KF
03
-8
0
KF
-80 2.5
-80 2
1
KF
103
106
105
KF
-8
KF
04
-8
03 L
L
104
4
1
2
103
102
102
10
5
6
7
8
9
10
11
12
13
5
6
7
Elution volume (mL)
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
8
9
10
11
12
13
5
10
15
20
25
min
Elution volume (mL)
Shodex GPC KF-800 series
THF
1.0mL/min
RI
Room temp.
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Shodex GPC KF-800L series
THF
1.0mL/min
RI
Room temp.
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Shodex GPC KF-801 x 2
THF
1.0mL/min
UV(280nm)
40˚C
Standard polystyrenes
7
Sample : 50μL
1. Polystyrene (Mw : 133,000)
2. Polystyrene (Mw : 55,100)
3. Polystyrene (Mw : 19,600)
4. Polystyrene (Mw : 7,210)
5. Polystyrene (Mw : 3,070)
6. Polystyrene (Mw : 580)
7. Toluene
(Mw: 92)
1 2
3
0.1%
0.1%
0.1%
0.1%
0.1%
0.1%
0.15%
Sample : 50μL
1. Polystyrene (Mw : 19,825,000)
2. Polystyrene (Mw : 1,320,000)
3. Polystyrene (Mw : 133,000)
4. Polystyrene (Mw : 7,210)
5. Polystyrene (Mw : 580)
6. Toluene
(Mw : 92)
6
4
0.05%
0.1%
0.1%
0.1%
0.1%
0.1%
3
4
5
2
5
Organic SEC (GPC) Columns (General Analysis) : THF
Molecular weight (Polystyrene)
06
106
L
07
-8
L
KF 8 0 6
L
KF 805
KF
07
-8
Sample : 100μL
1. Vitamin A palmitate
500μg/mL
2. Vitamin E
50μg/mL
3. Vitamin D3
50μg/mL
4. Vitamin K3
50μg/mL
3
107
KF
KF
10
Fat-soluble vitamins
Standard Analysis
Columns
Calibration curves for KF-800 series
using polystyrene
6
1
0
5
10
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
15
20
25
30 min
0
Shodex GPC KF-803L x 2
THF
1.0mL/min
UV(254nm)
40˚C
Styrene isoprene ABA block copolymer
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
20
25
Shodex GPC KF-807L x 2
THF
1.0mL/min
UV(254nm)
40˚C
Sample : Rubber 0.1%, 300μL
Sample : Polycarbonate resin 0.1%, 100μL
15
CH3
C
CH3
O C
O n
20
25
min
0
10
20
30 min
25 min
Column
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
30 min
Raw rubber
O
20
15
Polycarbonate resin
Sample : Styrene isoprene ABA block copolymer
15
10
Shodex GPC KF-806M x 2
THF
1.0mL/min
RI
30˚C
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Shodex GPC KF-806L x 2
THF
1.0mL/min
RI
40˚C
: Shodex GPC KF-806M x 2
+ KF-802
Toluene
1.0mL/min
RI
Room temp.
Eluent
:
Flow rate
:
Detector
:
Column temp. :
37
Standard Analysis
Columns
Organic SEC (GPC) Columns (General Analysis) :
Chloroform
Features
K-800
Standard organic solvent SEC (GPC) column
Supports a wide range of applications from low to high molecular weight compounds
Preparative
Columns
p.83
Standard columns
Organic SEC (GPC) Columns (General Analysis) : Chloroform
K-800 series : Shipping solvent Chloroform
Product Code
Product Name
Plate Number
(TP/column)
Exclusion Limit
(Polystyrene)
Particle Size
(µm)
Maximum
Pore Size
(Å)
Column Size
(mm)
I.D. x Length
F6028110
GPC K-801
≥ 18,000
1,500
6
50
8.0 × 300
F6028120
GPC K-802
≥ 18,000
5,000
6
150
8.0 × 300
F6028125
GPC K-802.5
≥ 18,000
20,000
6
300
8.0 × 300
F6028130
GPC K-803
≥ 18,000
70,000
6
500
8.0 × 300
F6028194
GPC K-803L
≥ 18,000
70,000
6
500
8.0 × 300
F6028140
GPC K-804
≥ 18,000
400,000
7
1,500
8.0 × 300
F6028195
GPC K-804L
≥ 18,000
400,000
7
1,500
8.0 × 300
F6028150
GPC K-805
≥ 11,000
4,000,000
10
5,000
8.0 × 300
F6028196
GPC K-805L
≥ 11,000
4,000,000
10
5,000
8.0 × 300
F6028160
GPC K-806
≥ 11,000
(20,000,000)*
10
10,000
8.0 × 300
F6028190
GPC K-806M
≥ 13,000
(20,000,000)*
10
10,000
8.0 × 300
F6028197
GPC K-806L
≥ 11,000
(20,000,000)*
10
10,000
8.0 × 300
F6028170
GPC K-807
≥ 6,000
(200,000,000)*
18
20,000
8.0 × 300
F6028198
GPC K-807L
≥ 6,000
(200,000,000)*
18
20,000
8.0 × 300
F6700401
GPC K-G
(guard column)
−
8
−
4.6 × 10
F6709450
GPC K-800D
−
10
−
8.0 × 100
solvent-peak
separation column
* See page 40 for details of the solvent-peak separation columns. The columns with ‘L’ or ‘M’ at
the end of column names are mixed-gel column capable of analyzing samples over a wide range
of molecular weight distribution.
Base Material : Styrene divinylbenzene copolymer
( )* Estimated value
* See page 50 for applicability of SEC (GPC) columns to solvent replacement.
Molecular weight range with polystyrene (eluent : chloroform)
Molecular weight (Polystyrene)
10
102
103
104
105
106
107
K-801
K-802
K-802.5
K-803
K-804
K-805
K-806
K-807
K-803L
K-804L
K-805L
K-806M
K-806L
K-807L
Calibration
38
Standards
See page 51 for
Calibration Standards
*Contact Shodex or our distributors near you for customized columns.
108
109
Sample : Epikote® 1001 0.1%, 100μL
Standard Analysis
Columns
Low molecular polydimethylsiloxanes
Epoxy resin
3
Sample : 0.1% each, 100μL
1. Polydimethylsiloxane (MW:770)
2. Polydimethylsiloxane (MW:237)
3. Hexamethyldisiloxane (MW:162)
2
10
15
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
20 min
0
Shodex GPC K-803L x 2
Chloroform
1.0mL/min
UV(254nm)
Room temp.
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
15
20
25
min
Shodex GPC K-801 x 2
Chloroform
1.0mL/min
RI(Polarity : negative)
40˚C
TM
Bionolle (Polybutylene succinate/adipate)
Silicon oil
Sample : Silicone oil 0.1%, 200μL
Sample : Polybutylene succinate/adipate 0.2%, 100μL
Silicone oil
10
15
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
20
25 min
15
Shodex GPC K-806M x 2
Toluene
1.0mL/min
RI
45˚C
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
20
25
Organic SEC (GPC) Columns (General Analysis) : Chloroform
1
30 min
Shodex GPC K-806M x 2 + K-801
Chloroform
1.0mL/min
RI
40˚C
Recycling fractionation of lauryl stearyl thiodipropionate
Sample : 5%, 500μL
1. Distearyl thiodipropionate
2. Lauryl stearyl thiodipropionate
3. Dilauryl thiodipropionate
CYCLE1
CYCLE2
CYCLE6
CYCLE10
2
1
0
10
20 min
3
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Shodex GPC K-LG + K-2001
Chloroform
3.0mL/min
RI(preparative type)
50˚C
* See page 83 for K-2001
39
Standard Analysis
Columns
Organic SEC (GPC) Columns (General Analysis) :
DMF
Features
KD-800
Standard organic solvent SEC (GPC) column
Supports a wide range of applications from low to high molecular weight compounds
Standard columns
KD-800 series : Shipping solvent Dimethylformamide (DMF)
Organic SEC (GPC) Columns (General Analysis) : DMF
Product Code
Plate Number
(TP/column)
Product Name
Maximum
Pore Size
(Å)
Particle Size
(µm)
Exclusion Limit
(PEG/PEO)*
Column Size (mm)
I.D. x Length
F6028210
GPC KD-801
≥ 17,000
2,500
6
50
8.0 × 300
F6028220
GPC KD-802
≥ 17,000
5,000
6
150
8.0 × 300
F6028225
GPC KD-802.5
≥ 17,000
20,000
6
300
8.0 × 300
F6028230
GPC KD-803
≥ 17,000
70,000
6
500
8.0 × 300
F6028240
GPC KD-804
≥ 17,000
400,000
7
1,500
8.0 × 300
F6028250
GPC KD-805
≥ 11,000
4,000,000
10
5,000
8.0 × 300
F6028260
GPC KD-806
≥ 11,000
(20,000,000)**
10
10,000
8.0 × 300
F6028290
GPC KD-806M
≥ 13,000
(20,000,000)**
10
10,000
8.0 × 300
F6028270
GPC KD-807
≥ 6,000
(200,000,000)**
18
20,000
8.0 × 300
F6700411
GPC KD-G
(guard column)
−
8
−
4.6 × 10
* The columns with ‘M’ at the end of column names are mixed-gel column capable of
analyzing samples over a wide range of molecular weight distribution.
Base Material : Styrene divinylbenzene copolymer
*PEG : polyethylene glycol
*PEO : polyethylene oxide
( )** Estimated value
* See page 50 for applicability of SEC (GPC) columns to solvent replacement.
Molecular weight range with PEG/PEO (eluent : DMF)
Molecular weight (PEG/PEO)
102
103
104
105
106
107
108
109
Solvent-peak Separation Columns for Organic SEC (GPC)
KD-801
KD-802
KD-802.5
KD-803
KD-804
KD-805
KD-806
KD-807
KD-806M
Solvent-peak Separation Columns for
Organic SEC (GPC)
Features
KF-800D, K-800D
Use this column in combination with a linear column
By shifting the elution of monomers, polymer additives, and the solvent-peak in low molecular
region, it reduces interferences in the calculation of the molecular weight distribution of
polymers and oligomers
Solvent-peak separation columns
Product Code
Product Name
Column Combination
Particle Size
(µm)
Column Size (mm)
I.D. x Length
Shipping Solvent
F6709350
GPC KF-800D
KF-805L, 806L, 806M, 807L
10
8.0 × 100
THF
F6709450
GPC K-800D
K-805L, 806L, 806M, 807L
10
8.0 × 100
Chloroform
Base Material : Styrene divinylbenzene copolymer
40
*Contact Shodex or our distributors near you for customized columns.
Polyvinylpyrrolidones
Melamine formaldehyde resin
Sample : Polyvinylpyrrolidone(PVP) 0.2% each
(A) PVP(K-30) 400μL
(B) PVP(K-60) 500μL
(C) PVP(K-90) 500μL
108
107
Sample : 30μL
Melamine-formaldehyde resin 1%
KD
-8
0
KD 7
-8
06
-8
05
(A)
KD
105
3
6M
104
KD
-8
04
-80
0
-8
KD
KD
Molecular weight (PEG/PEO)
106
KD
(B)
-80
2.5
103
KD
-8
02
-8
01
6
7
8
(C)
9
10
11
12
10
Elution volume (mL)
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
0
Shodex GPC KD-800 series
DMF
1.0mL/min
RI
50˚C
10
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
20
Shodex GPC KD-806M x 2
10mM LiBr in DMF
1.0mL/min
RI
50˚C
20
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Celluloses
25 min
Shodex GPC KD-802 x 2
10mM LiBr in DMF
1.0mL/min
RI
50˚C
Phenoxy resin
Sample : 250μL
Phenoxy resin 0.2%
Sample : Cellulose ca. 0.05% each
100μL
107
Cellulose is known to be difficult to
dissolve. A cellulose solution is
prepared by repeating solvent
replacement. It is reported that the
long time required for dissolution
(1 to 60 days), depends on solvent
type, the crystallinity and
molecular weight of the sample.
106
Molecular mass
15
30 min
105
Organic SEC (GPC) Columns (General Analysis) : DMF
KD
102
10
Standard Analysis
Columns
Calibration curves for KD-800 series
using PEG / PEO
104
6
7
8
9
10
15
11
20
25 min
Elution volume (mL)
Shodex GPC KD-806M
1% LiCl in DMI
0.5mL/min
RI, MALS(Multi angle laser light scattering)
60˚C
* DMI 1,3-dimethyl-2-imidazolidinone
Potato starch
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Data provided by Dr. Masahiko Yanagisawa,
Isogai group,
Graduate School of Agricultural and Life Sciences,
The University of Tokyo
Sample : 100μL
Potato starch in DMSO 0.1%
* solved at 80˚C
Sample : Polyvinyl chloride
DOP
(Dioctyl phthalate)
Column
: Shodex GPC KD-806M
Eluent
: 10mM LiBr in (DMSO/DMF=75/25)
Flow rate
: 1.0mL/min
Detector
: RI
Column temp. : 50˚C
0
5
D
103
5
6
7
8
9
10
11
12
13
14
15
Retention volume (mL)
KF-806L + KF-800D
15
min
00
10
-8
104
102
5
KF
L+
06
L
06
15 min
-8
10
-8
5
105
KF
0
106
KF
KF-806L
0
Shodex GPC KD-806M x 2
10mM LiBr in DMF
1.0mL/min
RI
50˚C
Effects of solvent-peak separation column
Molecular weight (Polystyrene)
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Solvent-peak Separation Columns for Organic SEC (GPC)
103
10 min
Column : Shodex GPC KF-806L
GPC KF-806L + KF-800D
Eluent
: THF
Flow rate : 1.0mL/min
Detector : RI
41
Standard Analysis
Columns
Organic SEC (GPC) Columns :
Rapid Analysis, High Performance Analysis
Features
Organic SEC (GPC) Columns : Rapid Analysis, High Performance Analysis
KF-600
Approximately half of the analysis time compared with standard columns
The amount of solvent used is reduced to about a third
Improved applicability of solvent replacement
KF-400HQ
About 1.5 times better separation performance than standard columns, obtains higher resolution
About 4 times better sensitivity than that of standard columns, supports high sensitivity analysis
The amount of solvent used is reduced to about a third
News No.3, 10
Improved applicability of solvent replacement
Rapid analysis downsized columns
Use of the KF-600 series with semi-micro type devices is recommended.
KF-600 series
Product Code
Particle Size
(µm)
Exclusion Limit
(Polystyrene)
Plate Number
(TP/column)
Product Name
Maximum Pore Size
(Å)
Column Size (mm)
I.D. x Length
F6028091
GPC KF-601
≥ 17,000
1,500
3
50
6.0 × 150
F6028092
GPC KF-602
≥ 17,000
5,000
3
150
6.0 × 150
F6028093
GPC KF-602.5
≥ 17,000
20,000
3
300
6.0 × 150
F6028094
GPC KF-603
≥ 17,000
70,000
3
500
6.0 × 150
F6028095
GPC KF-604
≥ 16,000
400,000
3
1,500
6.0 × 150
F6028096
GPC KF-605
≥ 7,000
4,000,000
10
5,000
6.0 × 150
F6028097
GPC KF-606
≥ 7,000
(20,000,000)*
10
10,000
6.0 × 150
F6028098
GPC KF-606M
≥ 8,000
(20,000,000)*
10
10,000
6.0 × 150
F6028099
GPC KF-607
≥ 5,000
(200,000,000)*
18
20,000
6.0 × 150
F6700300
GPC KF-G
(guard column)
−
8
−
4.6 × 10
* The columns with ‘M’ at the end of column names are mixed-gel column capable of
analyzing samples over a wide range of molecular weight distribution.
Base Material : Styrene divinylbenzene copolymer
Shipping Solvent : Tetrahydrofuran (THF)
( )* Estimated value
* See page 50 for applicability of SEC (GPC) columns to solvent replacement.
High performance semi-micro columns
Use of the KF-400HQ series with semi-micro type devices is recommended.
KF-400HQ series
Product Code
Plate Number
(TP/column)
Product Name
Particle Size
(µm)
Exclusion Limit
(Polystyrene)
Maximum Pore Size
(Å)
Column Size (mm)
I.D. x Length
F6028111
GPC KF-401HQ
≥ 25,000
1,500
3
50
4.6 × 250
F6028112
GPC KF-402HQ
≥ 25,000
5,000
3
150
4.6 × 250
F6028114
GPC KF-402.5HQ
≥ 25,000
20,000
3
300
4.6 × 250
F6028116
GPC KF-403HQ
≥ 25,000
70,000
3
500
4.6 × 250
F6028118
GPC KF-404HQ
≥ 25,000
400,000
3
1,500
4.6 × 250
F6028119
GPC KF-405LHQ
≥ 10,000
4,000,000
10
5,000
4.6 × 250
F6028122
GPC KF-406LHQ
≥ 10,000
(20,000,000)*
10
10,000
4.6 × 250
F6700300
GPC KF-G
8
−
4.6 × 10
−
(guard column)
* The columns with ‘L’ at the end of column names are mixed-gel column capable of
analyzing samples over a wide range of molecular weight distribution.
Base Material : Styrene divinylbenzene copolymer
Shipping Solvent : Tetrahydrofuran (THF)
( )* Estimated value
* See page 50 for applicability of SEC (GPC) columns to solvent replacement.
Molecular weight range with polystyrene (eluent : THF)
Molecular weight (Polystyrene)
10
102
103
104
105
106
Molecular weight (Polystyrene)
107
KF-601
108
109
10
102
103
104
105
KF-401HQ
KF-602
KF-402HQ
KF-602.5
KF-402.5HQ
KF-603
KF-403HQ
KF-604
KF-404HQ
KF-605
KF-405LHQ
KF-606
KF-406LHQ
KF-607
KF-606M
42
Calibration
Standards
See page 51 for
Calibration Standards
*Contact Shodex or our distributors near you for customized columns.
106
107
108
109
107
7
10
106
KF
06
-6
KF
3
-60
6
-60
-6
M
KF
104
02
.5
KF
-6
02
KF
-6
102
1.0
1.5
KF
-4
03
HQ
-4
02
.5
HQ
104
KF
103
KF
-4
102
01
2.0
2.5
3.0
3.5
10
1.0
4.0
1.5
2.0
2.5
3.0
3.5
4.0
Elution volume (mL)
Elution volume (mL)
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
KF
-4
02
HQ
HQ
01
Shodex GPC KF-600 series
THF
0.5mL/min
UV(254nm)
Room temp.
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Shodex GPC KF-400HQ series
THF
0.3mL/min
UV(254nm)
Room temp.
Comparison of standard, rapid analysis, and high performance type columns
Sample : EPON1001 0.2%
By using KF-602.5, the analysis time is reduced to less than half
of that of KF-802.5. Thus KF-600 series enables rapid analysis.
On the other hand, KF-402.5HQ has a theoretical plate number
1.5 times larger than that of the standard column, thereby
improving resolution especially in the analysis of molecules that
have a small to medium molecular weight. Rapid analysis and
high performance type columns use less than one third of
solvent per analysis compared to standard type columns.
21mL
Standard type
KF-802.5 x 2
50µL injection
5.7mL
High performance type
KF-402.5HQ x 2
10µL injection
Column
Rapid analysis type
: Shodex GPC KF-802.5 x 2
GPC KF-402.5HQ x 2
GPC KF-602.5 x 2
Eluent
: THF
Flow rate
: (KF-802.5) 1.0mL/min
(KF-402.5HQ) 0.3mL/min
(KF-602.5) 0.6mL/min
Detector
: (KF-802.5) RI(conventional type)
(KF-402.5HQ, KF-602.5) RI(small cell volume)
Column temp. : 40˚C
6.0mL
KF-602.5 x 2
10µL injection
0
5
10
Styrene acrylonitrile copolymer
15
20
25 min
Phthalates
Liquid paraffin
Sample : Styrene-acrylonitrile (30:70) copolymer
Organic SEC (GPC) Columns : Rapid Analysis, High Performance Analysis
103
105
HQ
04
-4
05
KF
105
KF
-6
04
Molecular weight (Polystyrene)
-6
KF
Molecular weight (Polystyrene)
10
KF
6
Standard Analysis
Columns
Calibration curves for KF-400HQ series using polystyrene
Calibration curves for KF-600 series using polystyrene
Sample : Liquid paraffin 1%, 5μL
Sample : 10μL
1. Dioctyl phthalate
2. Dibutyl phthalate
3. Dipropyl phthalate
4. Diethyl phthalate
5. Dimethyl phthalate
6. Propylbenzene
7. Ethylbenzene
8. Toluene
9. Benzene
0.1%
0.1%
0.1%
0.1%
0.1%
0.2%
0.2%
0.2%
0.2%
4
5
678 9
3
2
1
6
8
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
12
Shodex GPC KF-606M x 2
10mM LiBr in DMF
0.5mL/min
RI(small cell volume)
40˚C
14
min
2
4
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
6
8
Shodex GPC KF-401HQ
Chloroform
0.3mL/min
RI(small cell volume)
40˚C
10 min
8
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
12
16
20 min
Shodex GPC KF-401HQ x 2
THF
0.3mL/min
UV(254nm) (small cell volume)
40˚C
43
Standard Analysis
Columns
Organic SEC (GPC) Columns :
Linear Calibration Type
Features
LF
Organic SEC (GPC) Columns : Linear Calibration Type
Employs a special packing material with a wide pore size distribution (multi pore type)
Highly linear calibration curve without inflection points
Molecular weight distribution can be determined with high precision
Enables analysis over a broad range of molecular weights (100 to 2,000,000)
Column for rapid analysis (LF-604) and column for high performance analysis (LF-404) enabling reduction in
solvent use are also available
Note
book
No.1
News
No.4, 40
Standard column
Product Code
Product Name
Plate Number
(TP/column)
Exclusion Limit
(Polystyrene)
Particle Size
(µm)
Maximum
Pore Size
(Å)
Column Size (mm)
I.D. x Length
≥ 17,000
2,000,000
6
3,000
8.0 × 300
(guard column)
−
6
−
4.6 × 10
GPC LF-804
F6021041
GPC LF-G
F6709621
Base Material : Styrene divinylbenzene copolymer
Shipping Solvent : Tetrahydrofuran (THF)
* See page 50 for applicability of SEC (GPC) columns to solvent replacement.
Rapid analysis downsized column
Product Code
Product Name
F6021042
GPC LF-604
F6709621
GPC LF-G
Use of the LF-604 with semi-micro type devices is recommended.
Plate Number
(TP/column)
Exclusion Limit
(Polystyrene)
Particle Size
(µm)
Maximum
Pore Size
(Å)
Column Size (mm)
I.D. x Length
≥ 9,000
2,000,000
6
3,000
6.0 × 150
(guard column)
−
6
−
4.6 × 10
Base Material : Styrene divinylbenzene copolymer
Shipping Solvent : Tetrahydrofuran (THF)
* See page 50 for applicability of SEC (GPC) columns to solvent replacement.
High performance semi-micro column
Product Code
Product Name
F6021043
GPC LF-404
F6709621
GPC LF-G
Use of the LF-404 with semi-micro type devices is recommended.
Plate Number
(TP/column)
Exclusion Limit
(Polystyrene)
Particle Size
(µm)
Maximum
Pore Size
(Å)
Column Size (mm)
I.D. x Length
≥ 14,000
2,000,000
6
3,000
4.6 × 250
(guard column)
−
6
−
4.6 × 10
Base Material : Styrene divinylbenzene copolymer
Shipping Solvent : Tetrahydrofuran (THF)
* See page 50 for applicability of SEC (GPC) columns to solvent replacement.
Molecular weight range with polystyrene (eluent : THF)
Molecular weight (Polystyrene)
10
102
103
104
105
106
LF-804, 604, 404
107
Calibration
Standards
See page 51 for
Calibration Standards
Scheme of linear calibration type packing
Connecting linear calibration type columns (LF series)
The linear calibration type columns cover a broad range
of molecular weights with only one kind of packing (column).
Connecting mixed-gel columns (KF-804L, etc.)
Connecting different grades columns
(KF-804 + KF-803 + KF-802, etc.)
44
*Contact Shodex or our distributors near you for customized columns.
Calibration curves for LF-604 and LF-404
using polystyrene
Polyurethane
Sample : Polyurethane 0.1%, 20μL
108
107
Molecular weight (Polystyrene)
106
105
104
103
102
5
6
7
8
9
10
11
106
LF-604
105
104
LF-404
103
102
1.0
12
1.5
Elution volume (mL)
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
2.0
Shodex GPC LF-804
THF
1.0mL/min
RI
40˚C
3.0
3.5
4.0
0
: Shodex GPC LF-604, LF-404
: THF
: (LF-604) 0.5mL/min
(LF-404) 0.3mL/min
Detector
: RI(small cell volume)
Column temp. : 40˚C
15
15
20 min
20
Shodex GPC LF-404 x 2
THF
0.3mL/min
RI(small cell volume)
40˚C
Polymethyl methacrylate
Sample : Nylon® 6/6 0.1%, 20μL
Sample : Xylan 0.1%, 100μL
10
10
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Polyamide (Nylon6/6)
5
5
Column
Eluent
Flow rate
Xylan
0
2.5
Elution volume (mL)
0
25 min
Column
Eluent
: Shodex GPC LF-804
: 20mM H3PO4 + 20mM LiBr in
(DMSO/DMF=80/20)
Flow rate
: 0.6mL/min
Detector
: RI
Column temp. : 50˚C
5
10
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Polyamic acid
15
20
Sample : 100μL
Polymethyl methacrylate
25
min
0
Shodex GPC LF-404
5mM CF3COONa in HFIP
0.15mL/min
RI(small cell volume)
40˚C
5
10
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
15
20
25
Organic SEC (GPC) Columns : Linear Calibration Type
Molecular weight (Polystyrene)
107
Standard Analysis
Columns
Calibration curve for LF-804 using
polystyrene
30
min
Shodex GPC LF-804 x 2
Methyl ethyl ketone
1.0mL/min
RI
40˚C
Comparison of polystyrenes separation with different numbers of LF-404
4
Sample : Poly(pyromellitic dianhydride-co-4,4'-oxydianiline), 100μL
Sample : 10μL
1. PS Mw : 1,030,000
2. PS Mw : 152,000
3. PS Mw :
66,000
4. PS Mw :
22,000
5. PS Mw :
5,050
6. PS Mw :
580
7. Ethylbenzene
5
LF-404 x 1
3
6
7
2
1
4
5
3
7
6
2
Column
: Shodex GPC LF-404 x n
Eluent
: THF
Flow rate
: 0.3mL/min
Detector
: RI(small cell volume)
Column temp. : 40˚C
1
LF-404 x 2
0
5
10
15
20
25
30
35
40
45
min
4
3
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Shodex GPC LF-804 x 2
30mM LiBr + 30mM H3PO4 in NMP
0.7mL/min
RI
50˚C
6
1
LF-404 x 3
0
5
7
5
2
10
15
20
25
30 min
45
Standard Analysis
Columns
Organic SEC (GPC) Columns :
High Temperature/Ultra High Temperature Analysis
Features
HT-800
Varied product lineup to support a wide range of molecular weights
UT-800
Dedicated to SEC analysis at high/ultra high temperatures with a maximum usable temperature of 210˚C
Suitable for the analysis of ultra high molecular weight polymer containing samples
Organic SEC (GPC) Columns : High Temperature/Ultra High Temperature Analysis
Standard columns
Product Code
Plate Number
(TP/column)
Product Name
Exclusion Limit *
(Polystyrene)
Usable
Temperature
(˚C )
Particle Size
(µm)
Maximum
Pore Size
(Å)
F6208700
GPC HT-803
≥ 7,000
70,000
100−150
13
500
8.0 × 300
F6208710
GPC HT-804
≥ 7,000
400,000
100−150
13
1,500
8.0 × 300
F6208720
GPC HT-805
≥ 7,000
4,000,000
100−150
13
5,000
8.0 × 300
F6208730
GPC HT-806
≥ 7,000
(20,000,000)**
100−150
13
10,000
8.0 × 300
F6208740
GPC HT-806M
≥ 7,000
(20,000,000)**
100−150
13
10,000
8.0 × 300
F6208770
GPC HT-807
≥ 4,000
(200,000,000)**
100−150
18
20,000
8.0 × 300
F6709410
GPC HT-G
(guard column)
−
100−150
13
−
8.0 × 50
F6208600
GPC UT-802.5
≥ 4,400
20,000
100−210
30
300
8.0 × 300
F6208610
GPC UT-806M
≥ 4,400
(20,000,000)**
100−210
30
10,000
8.0 × 300
F6208620
GPC UT-807
≥ 3,300
(200,000,000)**
100−210
30
20,000
8.0 × 300
F6709400
GPC UT-G
(guard column)
−
100−210
30
−
8.0 × 50
F6208390
GPC AT-806MS
≥ 6,000
(20,000,000)**
***
Ta −150
12
10,000
8.0 × 250
F6700280
GPC AT-G
(guard column)
−
***
Ta −150
15
−
8.0 × 50
Base Material : Styrene divinylbenzene copolymer
Shipping Solvent : Toluene
*Exclusion limit was measured with THF
( )** Estimated value
Ta*** : Ambient temperature
* The columns with ‘M’ at the end of column names are mixed-gel column capable of
analyzing samples over a wide range of molecular weight distribution.
Molecular weight range with polystyrene (eluent : THF)
Molecular weight (Polystyrene)
10
102
103
104
105
106
107
HT-803
HT-804
HT-805
HT-806
HT-807
HT-806M
UT-802.5
UT-806M
UT-807
AT-806MS
Calibration
46
Column Size
(mm)
I.D. x Length
Standards
See page 51 for
Calibration Standards
*Contact Shodex or our distributors near you for customized columns.
108
109
Effects of gel particle size in high temperature GPC columns
High temperature SEC columns are suitable for the analysis of high molecular weight polymers that are
difficult to be dissolved in ambient temperature solvents; examples of such polymers are polyethylene
and polypropylene. GPC UT-800 series, which are packed with large particle size (30 μm) gel, are
recommended for the analysis of macromolecules. The large particle size prevents potential molecular
shear degradation of the sample.
Column : UT-806M x 3
(Particle size : 30µm)
HT
-8
06
6
10
2
HT
-80
10
2
1
5
HT
5
Column : HT-806M x 2
(Particle size : 13µm)
-8
04
-8
HT
HT
104
1
06
-8
03
M
103
Shear degradation
Vo
15
Vo
20
25
30
35
102
10
5
6
7
8
9
10
11
12
40
min
10
20
25
min
Sample :
1. Polystyrene
(MW : 20,000,000)
2. High density polyethylene (HDPE-A)
Column : AT-806MS x 2
(Particle size : 12µm)
13
15
Elution volume (mL)
2
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
1
Shodex GPC HT-800 series
THF
1.0mL/min
RI
Room temp.
Column
Vo
10
: Shodex GPC UT-806M
HT-806M, AT-806MS
Eluent
: 0.1% BHT in o-Dichlorobenzene
Flow rate
: 1.0mL/min
Detector
: RI
Column temp. : 140˚C
Shear degradation
15
20
min
Low density polyethylene
High density polyethylene
Sample : High density polyethylene (HDPE-B)
Sample : Low density polyethylene (LDPE-C)
108
Molecular weight
107
RI
106
105
10
104
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
12
16
20
Shodex GPC UT-806M x 2
0.1% BHT in o-Dichlorobenzene
1.0mL/min
RI, MALS(Multi angle laser light scattering)
145˚C
15
20
min
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
25 min
Organic SEC (GPC) Columns : High Temperature/Ultra High Temperature Analysis
Molecular weight (Polystyrene)
107
Standard Analysis
Columns
Calibration curves for HT-800 series using
polystyrene
Shodex GPC HT-806M x 2
0.1% BHT in o-Dichlorobenzene
1.0mL/min
RI
140˚C
47
Standard Analysis
Columns
Organic SEC (GPC) Columns : HFIP
Features
HFIP-800
Columns exclusively for use with hexafluoroisopropanol (HFIP)
HFIP-600
Rapid analysis, solvent saving type
Organic SEC (GPC) Columns : HFIP
Standard columns
HFIP-800 series
Product Code
Plate Number
(TP/column)
Product Name
Exclusion Limit
(PMMA)*
Column Size
(mm)
I.D. x Length
Maximum
Pore Size
(Å)
Particle Size
(µm)
F6028530
GPC HFIP-803
≥ 12,000
30,000
10
500
8.0 × 300
F6028540
GPC HFIP-804
≥ 12,000
100,000
7
1,500
8.0 × 300
F6028550
GPC HFIP-805
≥ 10,000
1,000,000
10
5,000
8.0 × 300
F6028560
GPC HFIP-806
≥ 10,000
(10,000,000)**
10
10,000
8.0 × 300
F6028590
GPC HFIP-806M
≥ 10,000
(10,000,000)**
10
10,000
8.0 × 300
F6028570
GPC HFIP-807
≥ 4,000
(100,000,000)**
18
20,000
8.0 × 300
F6700500
GPC HFIP-LG
(guard column)
−
15
−
8.0 × 50
Base Material : Styrene divinylbenzene copolymer
Shipping Solvent : Hexafluoroisopropanol (HFIP)
*PMMA : Polymethylmethacrylate
( )** Estimated value
* The columns with ‘M’ at the end of column names are mixed-gel column capable of
analyzing samples over a wide range of molecular weight distribution.
Rapid analysis downsized columns
HFIP-600 series
Product Code
Use of the HFIP-600 series with semi-micro type devices is recommended.
Plate Number
(TP/column)
Product Name
Exclusion Limit
(PMMA)*
Column Size
(mm)
I.D. x Length
Maximum
Pore Size
(Å)
Particle Size
(µm)
F6021030
GPC HFIP-603
≥ 12,000
30,000
3
500
6.0 × 150
F6021040
GPC HFIP-604
≥ 12,000
100,000
3
1,500
6.0 × 150
F6021050
GPC HFIP-605
≥ 5,000
1,000,000
10
5,000
6.0 × 150
F6021060
GPC HFIP-606
≥ 5,000
(10,000,000)**
10
10,000
6.0 × 150
F6021080
GPC HFIP-606M
≥ 6,000
(10,000,000)**
10
10,000
6.0 × 150
F6021070
GPC HFIP-607
≥ 3,000
(100,000,000)**
18
20,000
6.0 × 150
F6700511
GPC HFIP-G
(guard column)
−
8
−
4.6 × 10
Base Material : Styrene divinylbenzene copolymer
Shipping Solvent : Hexafluoroisopropanol (HFIP)
*PMMA : Polymethylmethacrylate
( )** Estimated value
* The columns with ‘M’ at the end of column names are mixed-gel column capable of
analyzing samples over a wide range of molecular weight distribution.
Molecular weight range with PMMA (eluent : HFIP)
Molecular weight (PMMA)
102
103
104
105
106
107
108
109
HFIP-803, 603
HFIP-804, 604
HFIP-805, 605
HFIP-806, 606
HFIP-807, 607
HFIP-806M, 606M
Calibration
Standards
48
*Contact Shodex or our distributors near you for customized columns.
See page 51 for
Calibration Standards
Calibration curves for HFIP-600 series
using PMMA
Polyethylene terephthalate (PET)
Sample : PET 0.2%, 20μL
7
0
-6
6
60
P-
FI
3
103
2
10
102
0
10
5
7
6
8
9
10
11
12
1.0
1.5
Elution volume (mL)
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
2.0
2.5
3.0
3.5
5
Shodex GPC HFIP-800 series
HFIP
1.0mL/min
RI
Room temp.
Column
:
Eluent
:
Detector
:
Column temp. :
Shodex GPC HFIP-600 series
5mM CF3COONa in HFIP
RI(small cell volume)
40˚C
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Shodex GPC HFIP-606M x 2
5mM CF3COONa in HFIP
0.6mL/min
RI(small cell volume)
40˚C
Polybutylene terephthalate (PBT)
Sample : Polycaprolactum (Nylon® 6)
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
20
25
Sample : PBT 0.05%, 500μL
In SEC analysis using HFIP, some samples
may yield abnormal peaks as a result of ionic
interaction. In this case, ionic interaction can
be suppressed by adding sodium
trifluoroacetate to HFIP.
5mM CF3COONa in HFIP
HFIP
15
15
30 min
Shodex GPC HFIP-806M x 2
HFIP(solid line), 5mM CF3COONa in HFIP(broken line)
1.0mL/min
RI
40˚C
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Sample : Nylon® 6/10 0.2%, 20μL
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
Shodex GPC HFIP-606M x 2
5mM CF3COONa in HFIP
0.6mL/min
RI(small cell volume)
40˚C
Sample : Polyacetal 0.2%, 20μL
Mw=38,100
15
min
30 min
Shodex GPC HFIP-805 + HFIP-803
5mM CF3COONa in HFIP
1.0mL/min
RI
40˚C
Sample : Nylon® 6 0.2%, 20μL
Mw=29,300
5
20
Polyacetal
Polyamides (Nylon 6/10 and Nylon 6)
0
15
min
Elution volume (mL)
Polyamide (effects of salt addition)
10
10
4.0
Organic SEC (GPC) Columns : HFIP
Molecular weight (PMMA)
IP
HF
104
H
103
Mw=19,300
4
IP80
4
IP80
3
HF
105
60
06
HF
104
-8
06M
IP
IP
HF
HF
5
60
105
IPHF
106
-6
HFIP
106
6M
80
IP5
HF P-80
I
HF
Molecular weight (PMMA)
10
Standard Analysis
Columns
Calibration curves for HFIP-800 series
using PMMA
0
5
10
Mw=176,000
15
min
0
5
10
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Shodex GPC HFIP-606M x 2
5mM CF3COONa in HFIP
0.6mL/min
RI(small cell volume)
40˚C
15
min
49
Standard Analysis
Columns
Applicability of SEC (GPC) Columns to Solvent
Replacement
Product Name
Shipping Solvent : THF
KF-801
Shipping Solvent : DMF
Applicability of SEC (GPC) Columns to Solvent Replacement
KF-804
KF-601
KF-603
LF-804
KD-801
KF-802.5
KF-805
KF-602
KF-604
LF-604
KD-802
KD-805
KF-803L
KF-806
KF-602.5
KF-605
LF-404
KD-802.5
KD-806
KF-804L
KF-807
KF-606
KD-807
KF-806M
KF-607
KD-806M
KF-805L
KF-606M
KF-802
KF-803
KF-806L
KF-807L
Solvent
Shipping Solvent : Chloroform
K-801
Shipping Solvent : THF
K-804
KF-401HQ
KF-403HQ
K-802.5
K-805
KF-402HQ
KF-404HQ
K-803L
K-806
KF-402.5HQ
KF-405LHQ
K-804L
K-807
K-802
K-803
KF-406LHQ
K-806M
K-805L
K-806L
K-807L
Tetrahydrofuran(THF)
Chloroform
Carbon tetrachloride
Benzene
Toluene
p-Xylene
o-Dichlorobenzene(ODCB)
Trichlorobenzene(TCB)
Dioxane
Diethyl ether
Ethyl acetate
Acetone
Methyl ethyl ketone
Dimethylformamide(DMF)
*1
*1
*1
Dimethylacetamide(DMAc)
*1
*1
*1
*1
*1
Hexafluoroisopropanol(HFIP)
m-Cresol
o-Chlorophenol
Quinolin
N-Methylpyrrolidone(NMP)
*1
*1
*1
Dimethylsulfoxide(DMSO)
*1
*1
*1
30% m-Cresol/Chloroform
30% o-Chlorophenol/Chloroform
30% HFIP/Chloroform
Hexane
Acetonitrile
Methanol
Water
50
: Solvent replacement possible
: Solvent replacement possible, but this may cause column performance to deteriorate slightly
*1 : Usable at 40˚C or higher
: Solvent replacement not possible
KD-803
KD-804
Calibration Standards
for SEC
Calibration Standards for SEC
[ Polystyrene (PS) ]
Features
For organic solvent SEC (GPC)
Less branched polystyrene with anionic polymerization
Easily soluble in tetrahydrofuran (THF), chloroform, toluene, and o-dichlorobenzene (ODCB)
Kit type
Product Name
Contents
MW Range
F8601105
STANDARD SL-105
0.5g x 10 kinds
580-21,800
F8602105
STANDARD SM-105
0.5g x 10 kinds
1,270-2,700,000
F8603075
STANDARD SH-75
0.5g x 7 kinds
591,000-6,870,000
SL-105
Std. No.
S-22
S-13
S-10
S-6.9
S-4.9
S-2.9
S-2.2
S-1.3
S-0.7
S-0.5
SM-105
Mp
21,800
13,000
10,400
6,940
4,910
2,940
2,170
1,280
770
580
Mw/Mn
1.02
1.02
1.03
1.03
1.03
1.04
1.04
1.07
1.08
1.12
Std. No.
S-2704
S-1345
S-609
S-333
S-139
S-53
S-22
S-6.9
S-3.2
S-1.3
Structural formula of S series
C4H9
CH2
CH
Calibration Standards for SEC
Product Code
H
n
SH-75
Mp
2,700,000
1,350,000
609,000
333,000
139,000
52,500
21,800
6,940
3,180
1,270
Std. No.
S-6870
S-5190
S-3990
S-2350
S-1820
S-991
S-591
Mw/Mn
1.04
1.03
1.02
1.03
1.03
1.03
1.02
1.03
1.04
1.06
Mp
6,870,000
5,190,000
3,990,000
2,350,000
1,820,000
991,000
591,000
Mw/Mn
1.09
1.03
1.05
1.04
1.04
1.05
1.02
(Note)
Molecular weights (Mp, Mw/Mn)
of each kit may vary depending
on production lots.
[ Polymethylmethacrylate (PMMA) ]
Features
For organic solvent SEC (GPC)
Narrow molecular weight distribution range
Easily soluble in hexafluoroisopropanol (HFIP) and dimethylformamide (DMF)
Kit type
Product Code
F8604075
Product Name
STANDARD M-75
Contents
MW Range
0.5g x 7 kinds
2,890-955,000
Std. No.
M-955
M-509
M-202
M-63
M-20
M-6.8
M-2.9
(Note)
Molecular weights (Mp, Mw/Mn) of each
kit may vary depending on production lots.
[ Pullulan ]
Mp
955,000
509,000
202,000
63,000
20,300
6,830
2,890
Mw/Mn
1.05
1.03
1.02
1.02
1.03
1.10
1.10
Features
For aqueous SEC (GFC)
Unbranched pullulan standard
High solubility in water eliminates the possibility of recrystallization
Kit type
Product Code
F8400000
Product Name
STANDARD P-82
Contents
MW Range
0.2g x 8 kinds
5,900-708,000
Single type
Structural formula of P series
O CH2
H
H
O
H
OH H
OH
H
OH
H CH2OH H
O
H
OH H
O
H
H CH2OH H
O
H
OH H
O
OH
H
OH
n
Product Code
Product Name
Contents
Mp
Mw/Mn
F8402500
STD P-2500
0.2g
2,350,000
1.49
F8401300
STD P-1300
0.2g
1,220,000
1.37
F8400800
STD P-800
0.5g
708,000
1.27
F8400400
STD P-400
0.5g
344,000
1.15
F8400200
STD P-200
0.5g
200,000
1.11
F8400100
STD P-100
0.5g
107,000
1.13
F8400050
STD P-50
0.5g
47,100
1.07
F8400020
STD P-20
0.5g
21,100
1.09
F8400010
STD P-10
0.5g
9,600
1.09
F8400005
STD P-5
0.5g
5,900
1.09
F8400003
STD P-3
0.2g
2,890
1.12
F8400002
STD P-2
0.2g
2,150
1.11
F8400001
STD P-1
0.2g
1,420
1.18
Std. No.
P-800
P-400
P-200
P-100
P-50
P-20
P-10
P-5
Mp
708,000
344,000
200,000
107,000
47,100
21,100
9,600
5,900
Mw/Mn
1.27
1.15
1.11
1.13
1.07
1.09
1.09
1.09
(Note)
Molecular weights (Mp, Mw/Mn) of each kit
may vary depending on production lots.
51
Standard Analysis
Columns
Columns for Anion Exchange Chromatography
Features
QA-825,
Suitable for the analysis of relatively high molecular weight compounds,
such as proteins, peptides, DNA, and RNA
Usable under wide pH range between 2 and 12
DEAE-825
Note
book
Columns for Anion Exchange Chromatography
DEAE3N-4T
Preparative
Columns
p.80
Semi-micro
Micro Columns
p.77
Preparative
Columns
p.80
No.7
No.6
News
No.33
Non-porous base material
Supports UHPLC (available under hyperbaric conditions up to 30 MPa)
News
ES-502N 7C
WA-624
p.77
Non-porous base material
For rapid analysis
Note
book
DEAE-2B
Semi-micro
Micro Columns
No.42
Compared to IEC series columns, polyvinyl alcohol is used as base material
and this offers different separation pattern
Low hydrophobic interaction of proteins allows analysis under mild conditions
Suitable for anion exchange analysis of low molecular weight compounds
such as nucleotides
Standard columns
Strong anion exchange resin (Functional Group : Quaternary ammonium)
Product
Code
F6110011
Product Name
IEC QA-825
Ion Exchange
Capacity
(meq/g)
Base Material
Particle
Size
(µm)
Pore
Size
(Å)
Column Size
(mm)
I.D. x Length
Shipping Solvent
0.45
Polyhydroxymethacrylate
12
5,000
8.0 × 75
50mM Na2SO4 aq.
Weak anion exchange resin (Functional Group : Diethylaminoethyl)
Product
Code
Product Name
Ion Exchange
Capacity
(meq/g)
Base Material
Particle
Size
(µm)
Pore
Size
(Å)
Column Size
(mm)
I.D. x Length
Shipping Solvent
F6118255
IEC DEAE-825
0.6
Polyhydroxymethacrylate
8
5,000
8.0 × 75
50mM Na2SO4 aq.
F6112100
IEC DEAE3N-4T
0.4
Polyhydroxymethacrylate
2.5
−
4.6 × 35
H2O
F6112110
PIKESS DEAE-2B
0.4
Polyhydroxymethacrylate
2.5
−
2.0 × 50
H2O
F7640002
Asahipak ES-502N 7C
0.55
Polyvinyl alcohol
9
2,000
7.5 × 100
50mM 1,3-Diaminopropane
+ 50mM NaCl(pH10.0)
F6356240
AXpak WA-624
1.2
Polyhydroxymethacrylate
10
2,000
6.0 × 150
0.1M Sodium phosphate buffer
(pH3.0)/CH3CN=80/20
F6700245
AXpak WA-G
(guard column) Polyhydroxymethacrylate
10
−
4.6 × 10
0.1M Sodium phosphate buffer
(pH3.0)/CH3CN=80/20
52
*Contact Shodex or our distributors near you for customized columns.
Effects of eluent pH for DEAE-825
Sample : Human serum 0.5%, 200μL
1. IgG
4
2. Transferrin
3. (Unknown)
4. Serum albumin
pH6.0
pH7.0
1
2
3 4
2
Sample : 100μL
1. Conalbumin
2. Transferrin
3. Ovalbumin
4. Trypsin inhibitor
3
4
1
Standard Analysis
Columns
Proteins in human serum
0.1%
0.2%
0.2%
0.2%
1,2
pH10.0
0
5
10
20
min
pH8.0
3
0
5
10
15
20
min
3
4
pH9.0
2
3
3 4
2
4
2
1
1
0
15
30
5
10
15
45 min
20
min
0
5
10
15
20
min
0
5
10
15
20
min
Column
Eluent
: Shodex IEC DEAE-825
: (A); 20mM Piperazine-HCl buffer(pH6.0), 20mM Bis-Tris-HCl buffer(pH7.0)
20mM Tris-HCl buffer(pH8.0), 20mM Ethanolamine-HCl buffer(pH9.0)
20mM 1,3-Diaminopropane-HCl buffer(pH10.0)
(B); (A) + 0.5M NaCl
Linear gradient; (A) to (B), 20min
Flow rate
: 1.0mL/min
Detector
: UV(280nm)
Column temp. : 25˚C
Column
Eluent
: Shodex IEC QA-825
: (A); 20mM Tris-HCl buffer(pH8.6)
(B); (A) + 0.5M NaCl
Linear gradient; 100% (A) to 50% (B), 60min
Flow rate
: 1.0mL/min
Detector
: UV(280nm)
Column temp. : Room temp.
Comparison of porous DEAE-825 and non-porous DEAE3N-4T for protein separation
Gibberellin Isomers
2
IEC DEAE3N-4T is a weak anion exchange column, having diethylaminoethyl functional group modified on
non-porous gel. The non-porous gel enables rapid analysis of proteins and peptides. DEAE3N-4T is also
suitable for the analysis of small-volume samples, as it provides sharp peaks even with small injection volume.
2. GA14
Sample :
Gibberellin
CH3
HO
1
porous gel DEAE-825
non-porous gel DEAE3N-4T
3
2
HOOC
COOH
3
1
: Diethylaminoethyl
: Diethylaminoethyl
1. endo GA14
1
Columns for Anion Exchange Chromatography
1
0
15
CH3
2
0
5
10
15
20
25
30 min
0
Sample :
1. Conalbumin
0.4mg/mL
2. Ovalbumin
1.2mg/mL
3. Trypsin inhibitor 1.0mg/mL
HO
HOOC
5 min
0
Column
Eluent
: Shodex IEC DEAE-825
: (A); 20mM Piperazine-HCl buffer(pH6.0)
(B); (A) + 0.5M NaCl
Linear gradient; (A) to (B), 60min
Flow rate
: 1.0mL/min
Detector
: UV(280nm)
Column temp. : Room temp.
Injection vol. : 100μL
Mercaptoalbumin and
non-mercaptoalbumin
1
2
3
Column
Eluent
: Shodex IEC DEAE3N-4T
: (A); 25mM Piperazine-HCl buffer(pH6.0)
(B); A + 0.5M NaCl
Linear gradient; (A) to (B), 10min
Flow rate
: 1.5mL/min
Detector
: UV(280nm)
Column temp. : Room temp.
Injection vol. : 20μL
Nucleotides
Sample : Normal control serum
1. Globulin
2. Uric acid
3. HMA
(Human Mercaptoalbumin)
4. HNA
(Human Nonmercaptoalbumin)
COOH
10
20 min
Column
Eluent
: Shodex Asahipak ES-502N 7C
: Acetic acid/H2O/CH3OH
=0.1/0.4/99.5
Flow rate
: 1.5mL/min
Detector
: UV(210nm)
Column temp. : 50˚C
Data was provided by Prof. Yamaguch,
Faculty of Agliculture, University of Tokyo.
Sulfide ion and cyanide ion
Sample : 20μL
1. 5'CMP 20μg/mL
2. 5'UMP 20μg/mL
3. 5'TMP 20μg/mL
4. 3'CMP 20μg/mL
5. 5'ADP 100μg/mL
6. 3'UMP 20μg/mL
7. 5'GMP 20μg/mL
8. 2'AMP 20μg/mL
9. 3'AMP 20μg/mL
10. 3'GMP 20μg/mL
2
Sample : 20μL
1. HS- 0.1mg/L
2. CN- 0.02mg/L
1
6
8
1
4
7
2
9
3
4
10
5
0.0 1.5 3.0 4.5 6.0 7.5 9.0 10.5 12.0 13.5 15.0 16.5 18.0
min
0
Column
Eluent
8
16
24 min
: Shodex Asahipak ES-502N 7C
: 50mM N-methylpiperazine-HCl
buffer(pH4.8) + 400mM Na2SO4 aq.
+ 0.3% C2H5OH
Flow rate
: 1.0mL/min
Detector
: UV(280nm)
Column temp. : 35˚C
0
5
Column
Eluent
10
15
20
25
30
min
: Shodex AXpak WA-624
: 0.35M CH3COOH aq.
/0.35M CH3COONH4 aq.=240/100
Flow rate
: 1.0mL/min
Detector
: UV(260nm)
Column temp. : 60˚C
Column
Eluent
: Shodex IEC DEAE-825
: 10mM Na2CO3
+ 1mM Ethylenediamine aq.
+ 10% CH3OH
Flow rate
: 1.0mL/min
Detector
: Electrochemical
(Electrode; Silver, 0mV SCE)
Column temp. : 25˚C
53
Standard Analysis
Columns
Columns for Cation Exchange Chromatography
Features
SP-825, CM-825
Suitable for the analysis of relatively high molecular weight compounds,
such as proteins, peptides, DNA, and RNA
Usable under wide pH range between 2 and 12
Note
book
No.7
Columns for Cation Exchange Chromatography
SP-420N
Non-porous base material
For rapid analysis
SP-2B
Non-porous base material
Supports UHPLC (available under hyperbaric conditions for up to 30 MPa)
Note
book
ES-502C 7C
No.6
News
P-421S
p.77
Preparative
Columns
p.81
Semi-micro
Micro Columns
p.77
Preparative
Columns
p.81
No.42
Compared to IEC series columns, polyvinyl alcohol is used as base material and
this offers different separation pattern
Low hydrophobic interaction with proteins allows analysis under mild conditions
Note
book
Semi-micro
Micro Columns
No.7
Column for amino acids analysis by cation exchange mode
Supports simultaneous analysis of different amino acids
Note
book
No.3
Standard columns
Strong cation exchange resin (Functional Group : Sulfopropyl)
Product
Code
Product Name
Ion Exchange
Capacity
(meq/g)
Base Material
Particle
Size
(µm)
Pore
Size
(Å)
Column Size
(mm)
I.D. x Length
Shipping Solvent
F6118250
IEC SP-825
0.4
Polyhydroxymethacrylate
8
5,000
8.0 × 75
50mM Na2SO4 aq.
F6113000
IEC SP-420N
0.3
Polyhydroxymethacrylate
2.5
−
4.6 × 35
20mM Sodium acetate buffer
+ 0.5M Na2SO4(pH5.0)
F6113110
PIKESS SP-2B
0.3
Polyhydroxymethacrylate
2.5
−
2.0 × 50
20mM Sodium acetate buffer
+ 0.5M Na2SO4(pH5.0)
Weak cation exchange resin (Functional Group : Carboxymethyl)
Product
Code
Product Name
Ion Exchange
Capacity
(meq/g)
Base Material
Particle
Size
(µm)
Pore
Size
(Å)
Column Size
(mm)
I.D. x Length
Shipping Solvent
F6110002
IEC CM-825
0.4
Polyhydroxymethacrylate
8
5,000
8.0 × 75
50mM Na2SO4 aq.
F7640001
Asahipak ES-502C 7C
0.55
Polyvinyl alcohol
9
2,000
7.5 × 100
0.1M Sodium phosphate buffer
(pH4.4)
For amino acids (Functional Group : Sulfo (Na+))
Product
Code
Product Name
F6354211
CXpak P-421S
F6700210
CXpak P-G
Particle Column Size
Size
(mm)
(µm) I.D. x Length
Plate Number
(TP/column)
Base Material
≥ 3,500
Styrene divinylbenzene copolymer
6
4.6 × 150
H2O
(guard column)
Styrene divinylbenzene copolymer
6
4.6 × 10
H2O
54
*Contact Shodex or our distributors near you for customized columns.
Shipping Solvent
( I ) CM-825
(Weak cation exchange)
90µL injected
Rapid analysis of proteins using UHPLC
( II ) SP-825
(Strong cation exchange)
30µL injected
( III ) SP-420N
(Strong cation exchange)
non-porous type gel
Sample : 5μL (13mg total protein )
1. Myoglobin
2. Trypsinogen
3. Ribonuclease A
4. α-Chymotrypsinogen A
5. Cytochrome c
6. Lysozyme
4
6
1
5
5
1
4
2
5
3
5
Standard Analysis
Columns
Protein separation using cation exchange columns
4
3
6
3
1
6
2
6
4
2
3
2
0.0
0
15
30
45 min
0
15
30 min
0
Column
Eluent
2
: ( I ) Shodex IEC CM-825, ( II ) IEC SP-825, ( III ) IEC SP-420N
: (A); 20mM Sodium phosphate buffer(pH7.0) (B); (A) + 0.5M NaCl
( I , II ) Linear gradient; (A) to (B), 60min ( III ) Linear gradient; (A) to (B), 10min
Flow rate
: ( I , II )1.0mL/min ( III )1.5mL/min
Detector
: UV(280nm)
Column temp. : Room temp.
Catecholamines
1
4
6 min
Sample : 300μg/mL each, 10μL
Sample :
1. Myoglobin
2. Trypsinogen
3. Ribonuclease A
4. α-Chymotrypsinogen A
5. Cytochrome c
6. Lysozyme
+
Asp-Arg-Val-Tyr-lle-His-Pro-Phe
+
N CH3 2Cl-
H3C N
2. Angiotensin I (human)
COOH
Asp-Arg-Val-Tyr-lle-His-Pro-PheHis-Leu
2
2. Adrenaline
HO
HO
Sample : 20μL
Paraquat
CH2·CH·NH2
2
3
4.0
min
Paraquat and diquat
1. Angiotensin II (human)
HO
HO
3.0
: Shodex PIKESS SP-2B
: (A); 20mM Sodium phosphate
buffer(pH 7.0)
(B); (A) + 0.5 M NaCl
Linear gradient; 100%(A) to 50%(B),
2.5min
Flow rate
: 1.2mL/min
Detector
: UV(280nm)
Column temp. : 25˚C
Sample : 10μL
1. DOPA
2.0
Column
Eluent
Angiotensins
1
1.0
CH·CH2·NH
OH
3. Angiotensin III (human)
CH3
Diquat
Arg-Val-Tyr-lle-His-Pro-Phe
Columns for Cation Exchange Chromatography
1
3. Noradrenaline
4
HO
HO
CH·CH2·NH2
N+
OH
N+
2Br-
4. Dopamine
HO
HO
CH2·CH2·NH2
3
0
20 min
0
10
20
0
30 min
Column
Eluent
: Shodex Asahipak ES-502C 7C
: 50mM Sodium malonate buffer(pH6.0)
/CH3CN=80/20
Flow rate
: 1.0mL/min
Detector
: UV(280nm)
Column temp. : 30˚C
Column
Eluent
: Shodex Asahipak ES-502C 7C
: 20mM Sodium malonate buffer
+ 0.5M NaCl(pH6.0)
Flow rate
: 1.0mL/min
Detector
: UV(280nm)
Column temp. : 30˚C
20 min
Column
Eluent
: Shodex Asahipak ES-502C 7C
: 50mM Sodium phosphate buffer
+ 150mM NaCl(pH7.0)
Flow rate
: 1.0mL/min
Detector
: UV(288nm)
Column temp. : 30˚C
Standard amino acids
Cys
Analysis of pochonicine and their analogues in filamentous
fungi culture extract
10
Sample : 5μL
Sample : 2.5nmol each, 100μL
Fraction purified from the culture extract of
Pochonia suchlasporia var. suchlasporia TAMA 87
Analogue B
0
0
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
15
20
25
30
35 min
Shodex Asahipak ES-502C 7C
10mM Ammonium bicarbonate aq.
0.6mL/min
UV(210nm)
40˚C
Data provided by Dr. Teruhiko Nitoda, Faculty of Agriculture, Okayama University.
NH3
Arg
Lue
Tyr
Phe
Lys
Ala
Pro
Analogue A
Ile
Glu
Pochonicine
Val
Met
Thr
Ser
Asp
Gly
His
(Fungi culture provided by Dr. Toru Okuda,
Tamagawa University Research Institute)
10
20
30
40
50 min
Column
Eluent
: Shodex CXpak P-421S
: No.1 0.12M Sodium citrate buffer(pH3.3)
No.2 0.13M Sodium citrate buffer(pH3.2)
No.3 0.11M Sodium citrate buffer(pH4.0)
No.4 1.02M Sodium citrate buffer(pH4.9)
No.5 0.2M NaOH aq./Ethanol=90/10 (Rinse solution)
Low pressure gradient ;
No.1 (0min), No.2 (1.2min), No.3 (10.0min), No.4 (21.2min), No.5 (40min)
Flow rate
: 0.5mL/min
Detector
: VIS(570nm)
Post column method(Ninhydrin reaction 0.35mL/min, 120˚C)
Column temp. : 63˚C
55
Standard Analysis
Columns
Columns for Special Separation Modes
Column for Hydrophobic Interaction Chromatography
Features
PH-814
Separates proteins without denaturation
Applicable to samples obtained after ammonium sulfate fraction treatment
Standard column
Columns for Chiral Separation
Columns for High Temperature Reversed Phase Chromatography
[Columns for Special Separation Modes] Column for Hydrophobic Interaction Chromatography
Product
Code
Product Name
Functional Group
Particle Size
(µm)
Pore Size
(Å)
Column Size (mm)
I.D. x Length
F6110003
HIC PH-814
Phenyl
10
2,000
8.0 × 75
Shipping Solvent
H2O
Base Material : Polyhydroxymethacrylate
Columns for Affinity Chromatography
Features
AFpak
Rigid polymer-based packing materials enable high speed analysis
Functional group is modified with chemically stable ligand (spacer)
Minimum detachment of functional groups ensures highly repeatable analysis
Standard columns
Product
Code
Product Name
Ligand
F7118954
AFpak ADS-894
Dextran sulfate
30mg
18
8.0 × 50
50mM Sodium phosphate buffer
+ 0.02% NaN3(pH7.4)
F7118945
AFpak AHR-894
Heparin
5mg
18
8.0 × 50
10mM Tris-HCl buffer + 10mM NaCl
+ 0.02% NaN3(pH7.4)
F7118946
AFpak APA-894
Protein A
4mg
18
8.0 × 50
0.1M Sodium phosphate buffer
+ 0.5M NaCl + 0.02% NaN3(pH7.0)
F7113050
AFpak APG-894
Protein G
4–5mg
18
8.0 × 50
10mM Sodium phosphate buffer
+ 0.15M NaCl + 0.02% NaN3(pH7.4)
F7118959
AFpak AWG-894
Wheat germ agglutinin
(WGA)
14mg
18
8.0 × 50
0.1M Tris-HCl buffer + 0.15M NaCl
+ 0.2M N-Acetylglucosamine
+ 0.02% NaN3(pH7.4)
F7118964
AFpak ACH-494
Choline oxydase,
Acetylcholine esterase
–
18
4.6 × 10
10mM Phosphate buffer
+ 1.0M NaCl(pH7.4)
Ligand Load/Gel Particle Size Column Size (mm)
I.D. x Length
(g)
(µm)
Shipping Solvent
Base Material : Polyhydroxymethacrylate
Columns for Chiral Separation
Features
CDBS-453
Separates optical isomers by using their conformational compatibility differences
Versatile column for chiral separation
CRX-853
Separates optical isomers by using the differences in metal complex formation capacities of
functional group and metal ion in eluent and optical isomers
Suitable for amino acids, hydroxyl acids, and their derivatives
Standard columns
Columns for Affinity Chromatography
Particle Column Size
(mm)
Size
I.D. x Length
(µm)
Product
Code
Product Name
Functional Group
Base Material
F7146003
ORpak CDBS-453
β-Cyclodextrin derivative
Silica
3
4.6 × 150
1.0% Acetic acid + 0.2M NaCl aq.
/CH3CN=70/30
F7140040
ORpak CRX-853
L-amino acid derivative
Polyhydroxymethacrylate
6
8.0 × 50
0.25mM CuSO4 aq.
F6709300
ORpak CRX-G
(guard column)
L-amino acid derivative
Polyhydroxymethacrylate
6
4.6 × 10
0.25mM CuSO4 aq.
Shipping Solvent
Columns for High Temperature Reversed Phase Chromatography
Features
ET-RP1
Capable of high temperature analysis up to 150˚C
High temperature analysis improves column efficiency and enables rapid analysis
News
No.41
Standard columns
56
Product
Code
Product Name
Plate Number
(TP/column)
Functional Group
Particle Size
(µm)
Pore Size
(Å)
Column Size (mm)
I.D. x Length
Shipping Solvent
F7623001
ET-RP1 4D
≥ 11,000
Octadecyl
4
250
4.6 × 150
H2O/CH3CN=35/65
F7623003
ET-RP1 3D
≥ 9,000
Octadecyl
4
250
3.0 × 150
H2O/CH3CN=35/65
Base Material : Polyvinyl alcohol
*Contact Shodex or our distributors near you for customized columns.
Choline and acetylcholine
Lipoproteins in plasma
Standard Analysis
Columns
Protein separation by hydrophobic
interaction chromatography
1
Sample : 370μL
1. Cytochrome c
0.03%
2. Myoglobin
0.08%
3. Ribonuclease A 0.16%
4. Ovalbumin
0.16%
5. Lysozyme
0.04%
6. α-Chymotrypsinogen
5
0.05%
1
2
3
Sample : 10μL
1. Choline
5mg/L
2. Ethylhomocholine 10mg/L
3. Acetylcholine
10mg/L
3
After choline and acetylcholine
are separated using DE-413
polymer-based reversed
phase column, solutes are
passed through ACH-494 to
generate hydrogen peroxide.
The resulting hydrogen
peroxide is detected using an
electrochemical detector to
enable highly sensitive
analysis.
6
4
LDL
2
VLDL
Eluent A Eluent B
Eluent A Eluent B
0
10 20 30 min
0
Sample :
Lipoproteins(Sigma)
0
20
40
Column
Eluent
: Shodex HIC PH-814
: (A); 1.8M Ammonium sulfate + (B)
(B); 0.1M Phosphate buffer(pH7.0)
Linear gradient; (A) to (B), 60min
Flow rate
: 1.0mL/min
Detector
: UV(280nm)
Column temp. : Room temp.
10 min
Warfarin
Sample : Epinephrine 50μg/mL, 50μL
HO
N
H
0
: Shodex AFpak ADS-894
: (A); 50mM Sodium phosphate buffer
(pH7.4)
(B); (A) + 1.0M NaCl
Step gradient; (A) to (B)
Flow rate
: 1.0mL/min
Detector
: UV(280nm)
Column temp. : Room temp.
Lactic acids
Sample : Warfarin 20μg/mL, 20μL
CH3
10 20 30 40 min
Column
Eluent
O
Sample : Lactic acid 50μg/mL, 50μL
1. L-Lactic acid
2. D-Lactic acid
O
1
2
CHCH2COCH3
OH
OH
OH
0
5
10
15
20
25
min
Column
Eluent
: Shodex ORpak CDBS-453
: 0.05% Acetic acid + 0.2M NaCl aq.
/CH3CN=95/5
Flow rate
: 0.5mL/min
Detector
: UV(254nm)
Column temp. : 10˚C
0
5
10
15
20
25
30
35
min
0
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Column
Eluent
: Shodex ORpak CDBS-453
: 1.0% Acetic acid + 0.2M NaCl aq.
/CH3CN=80/20
Flow rate
: 0.6mL/min
Detector
: UV(310nm)
Column temp. : 16˚C
10
15
min
Shodex ORpak CRX-853
0.5mM CuSO4 aq.
1.0mL/min
UV(230nm)
50˚C
Comparison of ET-RP1’s column efficiencies (theoretical plate height) observed at
high and normal temperature conditions
Mandelic acids
high temp. (150˚C)
2.4mL/min
normal temp. (40˚C)
0.5mL/min
Reduced plate height *
Sample
1
2
2
3
3
2
40˚C
150˚C
Acetophenone
3.2
2.4
Benzene
3.6
2.3
* Plate height / particle diameter of the packed resin
4
4
1
0
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
10
15
20
Shodex ORpak CRX-853
0.25mM CuSO4 aq.
1.0mL/min
UV(230nm)
50˚C
25
min
0
1
Column
Eluent
2
1
3
min
0
2
4
6
: Shodex ET-RP1 4D
: (Left) H2O/CH3CN=50/50
(Right) H2O/CH3CN=75/25
Detector
: Photodiode array(210nm)
Column Oven : Polaratherm 9000 Series
(SandraSelerity Technonogies, Inc)
8
10
12
14
min
Columns for Affinity Chromatography
Sample :
1. Uracil
2. Pyridine
3. Acetophenone
4. Benzene
Sample : Mandelic acid 100μg/mL, 20μL
1. D-Mandelic acid
2. L-Mandelic acid
Columns for High Temperature Reversed Phase Chromatography
Epinephrines
5
Column
: Shodex RSpak DE-413
Post column : Shodex AFpak ACH-494
Eluent
: 0.1M H3PO4 +
300mg/L Sodium 1-decansulfonate +
65mg/L Tetramethylammonium chloride
(pH8.0 adjusted by 1.0M NaOH)
Flow rate
: 1.0mL/min
Detector
: Electrochemical(Electrode : Pt, 350mV SCE)
Column temp. : 37˚C
60 min
Eluent B
Columns for Chiral Separation
Eluent A
[Columns for Special Separation Modes] Column for Hydrophobic Interaction Chromatography
Ligand : Dextran sulfate
* See page 10 for DE-413
0
10 20 30 min
HDL
Note :
The eluent was introduced into the column
after being preheated and was cooled after
column elution, then introduced into the
detector.
Data provided by Research Institute for Chromatography bvba
57
Standard Analysis
Columns
Columns for Polymer-based Hydrophilic Interaction Chromatography (HILIC)
Aqueous/Organic SEC Columns
[Columns for LC/MS Analysis] Columns for Polymer-based Reversed Phase Chromatography
Organic SEC (GPC) Columns
58
Columns for LC/MS Analysis
Columns for Polymer-based Reversed Phase Chromatography
Product
Code
Plate Number Functional
(TP/column)
Group
Product Name
Base Material
Particle Pore
Size
Size
(µm)
(Å)
Column Size
(mm)
I.D. x Length
Shipping Solvent
F7622004
ODP2 HP-2B
≥ 3,000
−
Polyhydroxymethacrylate
5
40
2.0 × 50
H2O/CH3CN=55/45
F7622005
ODP2 HP-2D
≥ 7,000
−
Polyhydroxymethacrylate
5
40
2.0 × 150
H2O/CH3CN=55/45
F6714011
ODP2 HPG-2A
(guard column)
−
Polyhydroxymethacrylate
5
−
2.0 × 10
H2O/CH3CN=55/45
F7620009
Asahipak ODP-50 2D
≥ 5,000
Octadecyl
Polyvinyl alcohol
5
250
2.0 × 150
H2O/CH3CN=35/65
F6713001
Asahipak ODP-50G 2A (guard column) Octadecyl
Polyvinyl alcohol
5
−
2.0 × 10
H2O/CH3CN=35/65
F7001007
RSpak DE-213
≥ 8,000
−
Polymethacrylate
4
25
2.0 × 150
H2O/CH3CN=50/50
F6700151
RSpak DE-SG
(guard column)
−
Polymethacrylate
6
−
2.0 × 10
H2O/CH3CN=50/50
F7008160
RSpak NN-414
≥ 6,000
Sulfo
Polyhydroxymethacrylate
10
200
4.6 × 150
0.1M Sodium phosphate
buffer(pH3.0)
F7008220
RSpak JJ-50 2D
≥ 3,500
Quaternary
ammonium
Polyvinyl alcohol
5
100
2.0 × 150
H2O/CH3CN=40/60
Columns for Polymer-based Hydrophilic Interaction Chromatography
(HILIC)
Particle Pore
Size
Size
(µm)
(Å)
Column Size
(mm)
I.D. x Length
Shipping Solvent
100
2.0 × 50
CH3CN
4
100
2.0 × 150
CH3CN
Polyvinyl alcohol
4
100
2.0 × 250
CH3CN
Amino
Polyvinyl alcohol
5
100
2.0 × 150
H2O/CH3CN=25/75
Amino
Polyvinyl alcohol
5
−
2.0 × 10
H2O/CH3CN=25/75
Plate Number Functional
(TP/column)
Group
Product
Code
Product Name
F7630008
Asahipak NH2P-40 2B
≥ 2,000
Amino
Polyvinyl alcohol
4
F7630009
Asahipak NH2P-40 2D
≥ 5,500
Amino
Polyvinyl alcohol
F7630010
Asahipak NH2P-40 2E
≥ 7,000
Amino
F7630006
Asahipak NH2P-50 2D
≥ 3,500
F6713000
Asahipak NH2P-50G 2A
(guard column)
Base Material
* Asahipak NH2P-40 2B, Asahipak NH2P-40 2D, and Asahipak NH2P-40 2E are the new names for the NH2P40-2B, NH2P40-2D, and NH2P40-2E, respectively.
Aqueous/Organic SEC Columns
Base Material
Particle
Size
(µm)
Maximum
Pore Size
(Å)
Column Size
(mm)
I.D. x Length
Shipping
Solvent
40,000
Polyvinyl alcohol
5
400
4.6 × 50
H2O
≥ 10,000
40,000
Polyvinyl alcohol
5
400
4.6 × 150
H2O
MSpak GF-310 4E
≥ 16,000
40,000
Polyvinyl alcohol
5
400
4.6 × 250
H2O
MSpak GF-310 2D
≥ 5,500
40,000
Polyvinyl alcohol
5
400
2.0 × 150
H2O
Column Size
(mm)
I.D. x Length
Shipping
Solvent
Product
Code
Product Name
F7600100
MSpak GF-310 4B
≥ 3,000
F7600110
MSpak GF-310 4D
F7600024
F7600120
Plate Number Exclusion Limit
(TP/column)
(Pullulan)
Organic SEC (GPC) Columns
Product
Code
Product Name
Plate
Exclusion
Number
Limit
(TP/column) (Polystyrene)
Base Material
Particle Maximum
Size Pore Size
(µm)
(Å)
F6028111
GPC KF-401HQ
≥ 25,000
1,500
Styrene divinylbenzene copolymer
3
50
4.6 × 250
THF
F6028112
GPC KF-402HQ
≥ 25,000
5,000
Styrene divinylbenzene copolymer
3
150
4.6 × 250
THF
F6028114
GPC KF-402.5HQ
≥ 25,000
20,000
Styrene divinylbenzene copolymer
3
300
4.6 × 250
THF
F6700300
GPC KF-G
(guard column)
−
Styrene divinylbenzene copolymer
8
−
4.6 × 10
THF
*Contact Shodex or our distributors near you for customized columns.
ODP2 HP
For the LC/MS analysis of drug samples
containing protein as a matrix, using ODS
columns generally exhibit ion suppression
of the target drugs. Since proteins are not
retained by ODP2 HP column, they elute
separate (as a void) from the target
components. Thus, using ODP2 HP column
exhibits less ion suppression influence.
ODS
Barbital 500ng/mL
Barbital 500ng/mL
Barbital
Barbital
Peak Area : 100%
Peak Area : 100%
Column
: Shodex ODP2 HP-2B
ODS from other manufacturer
: 10mM Ammonium acetate aq.
/CH3CN=70/30
Flow rate
: 0.2mL/min
Detector
: ESI-MS(SIM Negative : m/z 183)
Column temp. : 30˚C
Injection vol. : 10μL
Eluent
0.0
1.0
Barbital
Barbital
Peak Area : 99%
Peak Area : 71%
2.0
3.0
4.0 min
0.0
1.0
LC/MS analysis of drugs in control serum
2.0
3.0
4.0 min
LC/MS analysis of statins
Sample : 100ng/mL each, 5μL
Sample : 5μL
Drugs 500ng/mL each in Control serum
Lidocaine
Pravastatin
m/z 235
m/z 423
Chlorpheniramine
Pitavastatin
m/z 275
Propranolol
m/z 260
m/z 422
Fluvastatin
m/z 412
Diphenhydramine
m/z 256
UV(280m)
Atorvastatin
m/z 559
Bupivacaine
m/z 289
Mevastatin
m/z 391
into MS
Verapamil
m/z 455
0
2
4
6
8
10
Imipramine
12 min
0
4
6
8
10
m/z 281
12
14 min
m/z 405
Simvastatin
m/z 419
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 min
Column
Eluent
: Shodex ODP2 HP-2B
: (A); 10mM Ammonium formate aq. (B); CH3CN
Linear gradient;
B%; 10%(0min) 10%(3min) 35%(13min) 35%(20min)
Flow rate
: 0.2mL/min
Detector
: ESI-MS(SIM)
Column temp. : 40˚C
Shodex ODP2 HP-2B
10mM Ammonium acetate aq./CH3CN=70/30
0.2mL/min
UV(280nm), ESI-MS(SIM Positive)
40˚C
LC/MS analysis of basic drugs
LC/MS analysis of organic acids
Sample : 50ng/mL each, 10μL
ODP-50 2D, a polymer-based reversed phase column, is capable of analyzing
basic drugs at low concentration or without salt. This is preferred for ESI-MS
analysis, since the presence of salt causes ion suppression, and thus influences
the analysis performance.
Glyoxylic acid
1
m/z 73
Pyruvic acid
m/z 87
Glycolic acid
m/z 75
Lactic acid
2
m/z 89
Maleic acid
m/z 115
Succinic acid
m/z 304
m/z 117
m/z 290
Fumaric acid
m/z 115
Adipic acid
0
1
2
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
3
4
5
6
Shodex Asahipak ODP-50 2D
H2O/CH3CN=40/60
0.2mL/min
ESI-MS(SIM Positive)
30˚C
7
8
9 min
0
1
2
3
4
5
6
7
m/z 145
8
Organic SEC (GPC) Columns
Sample : 10ng/mL each, 5μL
1. Scopolamine
2. Atropine
Aqueous/Organic SEC Columns
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
2
Lovastatin
Columns for Polymer-based Hydrophilic Interaction Chromatography (HILIC)
Barbital 500ng/mL in BSA 7.0mg/mL
[Columns for LC/MS Analysis] Columns for Polymer-based Reversed Phase Chromatography
Barbital 500ng/mL in BSA 7.0mg/mL
Standard Analysis
Columns
Barbital recovery rate comparison of ODP2 HP-2B and ODS in the presence of BSA
9 min
Column
Eluent
: Shodex RSpak DE-213
: (A); 0.1%(v/v) Formic acid aq. (B); CH3CN
Linear gradient;
B%; 5%(0min) 5%(2min) 15%(2.5min)
Flow rate
: 0.2mL/min
Detector
: ESI-MS(SIM Negative)
Column temp. : 30˚C
15%(10min)
59
Standard Analysis
Columns
LC/TOF-MS analysis of 2-amino benzoic acid derivatized
sugar chains
LC/MS analysis of N-acetyl-chitooligosaccharides
Sample : 5μL
Mixture of N-acetyl-chitooligosaccharides 500ng/mL
N-Acetyl-D-glucosamine
2
* 2AA : 2-Aminobenzoic acid
m/z 220
3
2mer
m/z 423
1
3mer
m/z 626
4mer
5
4
m/z 829
Columns for Polymer-based Hydrophilic Interaction Chromatography (HILIC)
Aqueous/Organic SEC Columns
[Columns for LC/MS Analysis] Columns for Polymer-based Reversed Phase Chromatography
6
5mer
m/z 1032
5
6mer
10
Migration time (min)
m/z 1235
0
1
2
3
4
5
6
7
8
9
10
11
12
13
: Shodex Asahipak NH2P-40 2D
: (A); 0.05% Ammonia aq. (B); CH3CN
Linear gradient; (B%) 75%(0min) 50%(10min)
Flow rate
: 0.2mL/min
Detector
: ESI-MS(SIM Negative)
Column temp. : 30˚C
15
20
Column
Eluent
: Shodex Asahipak NH2P-40 2D
: (A); 95% CH3CN/0.1% Formic acid aq.
(B); 5% CH3CN/0.1% Formic acid aq.
Linear gradient; (B%) 30%(0-2.5min), 30-95%(2.5-20min)
Flow rate
: 0.2mL/min
Detector
: ESI-TOF MS(Polarity : Negative, Full MS range : 2000 )
Column temp. : 45˚C
14 min
Column
Eluent
50%(15min)
Data provided by Michihiro Kinoshita Ph.D., Faculty of Pharmacy, Kinki University
LC/MS analysis of creatinine and creatine
LC/MS analysis of melamine and cyanuric acid
1
Sample : 100ng/mL each, 5μL
1. Creatinine
2. Creatine
H2N
N
N
NH
O
CH3
NH
N
Sample : 500ng/mL each, 5μL
1. Melamine
2. Cyanuric acid
NH2
N
2
1
O
NH2
N
H2N
2
Melamine
OH
CH3
HO
OH
N
NH
Creatinine
Creatine
N
N
OH
Cyanuric acid
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19 min
0
Column
Eluent
: Shodex Asahipak NH2P-40 2D
: (A); 50mM Ammonium formate aq. (B); CH3CN
Linear gradient;
B%, 90%(0min) 90%(6min) 75%(7min) 75%(20min)
Flow rate
: 0.2mL/min
Detector
: Creatinine : ESI-MS, Creatine : ESI-MS/MS
Column temp. : 30˚C
1
2
Column
Eluent
Flow rate
Detector
3
4
5
6
7
8
9 10 11 12 13 14 15 16 17 18 19 min
:
:
:
:
Shodex Asahipak NH2P-40 2D
50mM Ammonium formate aq./CH3CN=25/75
0.2mL/min
Melamine : ESI-MS/MS
Cyanuric acid : ESI-MS
Column temp. : 30˚C
LC/MS/MS analysis of estradiol metabolites
1
Sample : 0.1ppm each, 5μL
1. Estrone-3-sulfate
2. β-Estradiol-3-sulfate
3. Estriol-3-sulfate
4. Dehydroepiandrosterone-3-sulfate
5. Estrone-3-glucuronide
6. β-Estradiol-17-glucuronide
7. β-Estradiol-3-glucuronide
8. Estriol-3-glucuronide
9. Estriol-16-glucuronide
10. Dehydroepiandrosterone-3-glucuronide
11. Androsterone-3-glucuronide
2
3
Organic SEC (GPC) Columns
5
6
9
4
11
7
8
10
Column
Eluent
: Shodex Asahipak NH2P-40 2D
: (A); 10mM NH4OH CH3CN
(B); 10mM NH4OH H2O
Linear gradient;
B%, 22%(0-5min), 22-70%(5-10min),
70%(10-15min)
Flow rate
: 0.2mL/min
Detector
: ESI-MS/MS(Polarity : Negative)
Column temp. : 40˚C
0
60
Sample : 2μL
Human IgG (1μg protein)
2AA-labeled N-Glycan
5
Time/min
10
15
Data provided by Mr. Atsushi Yamamoto,
Osaka City Institute of Public Health and Environmental Sciences
LC/MS analysis of perchloric acid and oxyhalides
Sample : Arsenic compounds, 50μL
1. Monomethylarsonic acid
2. Arsinic acid
3. Dimethylarsinic acid
4. Arsenobetaine
5. Tetramethylarsonium
6. Trimethylarsine oxide
2000
Standard arsenic compounds
1
1000
3
2
4
5
ClO4-
m/z 99
ClO3-
6
0 100 200 300 400 500 600 700 800 900 1000 1100 1200
sec
m/z 83
Urine of a healthy person
2000
BrO3-
4
m/z 127
4
1000
Cl-
1000
1
3
2
1
0 100 200 300 400 500 600 700 800 900 1000 1100 1200
Column
Eluent
Flow rate
Detector
:
:
:
:
sec
Shodex RSpak NN-614
5mM HNO3 /8mM NH4NO3 aq.
0.8mL/min
IC-ICP-MS(SIM m/z 75)
m/z 35
3
5
6
100 200 300 400 500 600 700 800 900 1000 1100 1200
0
Sample : Nonylphenol ethoxylates, 20μL
10
15
20 min
sec
Source:
Noriko Tsunoda,
Pharmacia. 1998, vol.34, No.12, p.1237-1241
LC/MS analysis of nonylphenol ethoxylates
5
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Shodex RSpak JJ-50 2D
50mM Ammonium formate aq./CH3CN=25/75
0.2mL/min
ESI-MS(SIM Negative)
30˚C
LC/MS analysis of additives
The analysis of additives that are present in polymers usually
require sample pretreatment steps. However, by using size
exclusion chromatography columns, easy and high sensitive
analysis of additives was achieved without any pretreatment
steps. The target additives are separated from the polymer and
this allows introducing only the low molecular fraction (which
contains the additives) into MS.
Sample : Cup of instant noodles (styrene foam) 1000mg/L, 5μL
UV(210nm)
0
1
2
3
4
5
6
7
8
9
10 11
12
13 14
15
16
min
Aqueous/Organic SEC Columns
not detected
Columns for Polymer-based Hydrophilic Interaction Chromatography (HILIC)
2
[Columns for LC/MS Analysis] Columns for Polymer-based Reversed Phase Chromatography
Urine from a patient with
arsenic poisoning
2000
Sample : 5μL
ClO4- 0.2μg/L
ClO3- 5μg/L
BrO3- 5μg/L
Cl1mg/L
Standard Analysis
Columns
Speciation of arsenic
IRGANOX ® 1010
Column
Eluent
Flow rate
Detector
: Shodex MSpak GF-310 4D
: (A) CH3CN, (B): H2O
A/B=30/70(7min) (23min) A/B=90/10
: 0.5mL/min
: ESI-MS(SIM Positive)
Source:
Mr. Yoshinari Kobuke (Environmental Science Institute of Hyogo Prefecture),
Basic Study of Chemical Substances in LC/MS,
10th Symposium on Environmental Chemistry Program and Abstracts, 542-543, 2001.
0
1
2
3
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
4
5
6
7
8
9
10 11
12 13 14 15
16
min
Organic SEC (GPC) Columns
m/z 1176
(Negative)
Shodex GPC KF-402HQ x 2
THF
0.3mL/min
UV(210nm), APCI-MS(SIM)
40˚C
61
Sample Pretreatment
Sample
Pretreatment
Columns
Columns
Columns for GPC Clean-up
Features
EV
Suitable for fractionation of residual pesticides in food
Used in the purification process of test solution preparation method in Shoku-An No. 1003001 (October 3rd,
2006. Japan) of the Pharmaceutical and Food Safety Bureau, MHLW, Section 2 "Simultaneous GC/MS
(LC/MS) Analyses of Agricultural Chemicals in Livestock and Marine Products"
Columns for GPC Clean-up
PAE
Suitable for cleaning up high-moisture samples such as blood and bottom sediment
Highly effective for fractionation of endocrine disruptors in environmental samples
Eluent
No
Appropriate columns
for GPC Clean-up
Does the sample
contain water?
Yes
Column
Ethylacetate/Cyclohexane
EV-2000, EV-200
Acetone/Cyclohexane
EV-2000 AC, EV2000AC-12F
Acetonitrile
PAE-2000, PAE-800
Acetone
PAE-2000 AC, PAE-800 AC
GPC Clean-up for residual pesticides in food, etc.
Product Code
Product Name
Particle Size
(µm)
Maximum
Pore Size
(Å)
Column Size
(mm)
I.D. x Length
Shipping Solvent
F6090006
CLNpak EV2000AC-12F
16
30
12.0 × 300
Acetone/Cyclohexane=3/7
F6090007
CLNpak EV-G AC12C
16
(guard column)
12.0 × 100
Acetone/Cyclohexane=3/7
F6090003
CLNpak EV-2000 AC
16
30
20.0 × 300
Acetone/Cyclohexane=3/7
F6090004
CLNpak EV-G AC
16
(guard column)
20.0 × 100
Acetone/Cyclohexane=3/7
F6090005
CLNpak EV-200
16
30
2.0 × 150
Ethylacetate/Cyclohexane=3/7
F6090001
CLNpak EV-2000
16
30
20.0 × 300
Ethylacetate/Cyclohexane=3/7
F6090002
CLNpak EV-G
16
(guard column)
20.0 × 100
Ethylacetate/Cyclohexane=3/7
Base Material : Styrene divinylbenzene copolymer
GPC Clean-up for phthalic acid esters in sediments, biological samples, blood, etc.
Product Code
Product Name
Particle Size
(µm)
Maximum
Pore Size
(Å)
Column Size
(mm)
I.D. x Length
Shipping Solvent
F7600025
CLNpak PAE-800
5
400
8.0 × 300
Acetonitrile
F6810022
CLNpak PAE-2000
5
400
20.0 × 300
Acetonitrile
F6714007
CLNpak PAE-G
9
(guard column)
8.0 × 50
Acetonitrile
F7600026
CLNpak PAE-800 AC
5
400
8.0 × 300
Acetone
F6810023
CLNpak PAE-2000 AC
5
400
20.0 × 300
Acetone
F6714008
CLNpak PAE-G AC
9
(guard column)
8.0 × 50
Acetone
Base Material : Polyvinyl alcohol
62
*Contact Shodex or our distributors near you for customized columns.
Extraction
Liver, Kidney
Muscle, Fat, Fish
Milk, Egg
Acetone/n-Hexane (1/2)
GPC Clean-up of residual pesticides using EV-2000 AC
Honey
Tricyclazole (5mg/L)
(marker pesticide)
Acrinathrin (5mg/L)
(marker pesticide)
Acetonitrile
Salting-out
GPC
Pesticide
fraction
Fraction-2
Fraction-1
[*1]
Pesticide
fraction
pesticide
fraction
muscle, fat,
fish, milk, egg
[*1]
Columns for GPC Clean-up
Purification
GPC
[*1]
fraction-2
liver, kidney
[*2]
fraction-1
Test sample
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Injection vol. :
GC/MS, LC/MS
*1 Purification with ethylenediamine-N-propylsilyled silica gel mini-column
*2 Purification with silica gel mini-column
GPC Clean-up of beef tallow
GPC Clean-up of fatty tuna
1
0
5
10
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Injection vol. :
2
15
20
25
Sample : 30μL
PCB std. KC-mixture
Insulating oil
dissolved by hexane
[Extraction]
5mg/L
5mg/L
30
1) Weigh 20.0g of the sample (5.0g for fat).
2) Add 20mL of water and homogenize.
3) Add 100mL of acetone/n-hexane (1/2)
and homogenize.
4) Centrifuge (2,500rpm, 5min.) and collect
the organic layer (1).
5) Add 50mL of n-hexane to the residue
and homogenize.
6) Centrifuge (2,500rpm, 5min.) and collect
the organic layer (2).
7) Combine (1) and (2) and add anhydrous
sodium sulfate for dehydration.
8) Remove anhydrous sodium sulfate by
filtering and concentrate the filtrate at
40˚C or lower.
9) Remove the solvent.
10) Weigh the residue (extracted fat).
11) Dissolve in acetone/cyclohexane (1/4).
400mg/L
5,000mg/L
Insulating oil
Acetone
Hexane
0
10
20
Shodex CLNpak EV-G AC + EV-2000 AC
Acetone/Cyclohexane=1/4
5.0mL/min
UV(254nm)
40˚C
5mL
Shodex CLNpak PAE-800
Methanol, Acetone, Hexane
0.8mL/min
Photodiode array(209nm)
40˚C
Source:
Mr. Tetsuya Sawatsubashi (Mitsubishi Heavy Industries, Ltd.) et
al., Search of Liquid Chromatographic Clean-up Materials for
Rapid PCB Analysis and Evaluation of Their Separation
Characteristics. Journal of Environmental Chemistry, 2007,
Vol. 17, No. 3, p. 471-481.
GPC Clean-up of carcinogens in diesel
dust measured using PAE-800 AC
Elution positions of phthalic acid esters
using PAE-800 AC
1,3-Dinitropyrene
Bisphenol A
Benzo[a]pyrene
PCBs
Hydroxybenzo[a]pyrene mixture
Sulfer
Corn oil
RI
RI
Time
14
56
15
60
16
64
17
68
18
72
19
76
30
Retention time (min)
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
Benzo[a]fluoranthene
Volume
PCB
Methanol
35 min
Pretreatment of PCBs in waste oil
Shodex CLNpak EV-G AC + EV-2000 AC
Acetone/Cyclohexane=1/4
5.0mL/min
UV(254nm)
40˚C
5mL
PCB and insulating oil using PAE-800
GPC Clean-up of fatty tuna and beef tallow
Sample : 5mL
Beef tallow
Fatty tuna
1. Acrinathrin
2. Tricyclazole
Sample Pretreatment
Columns
Sample preparation outline for simultaneous GC/MS and LC/MS analysis of
agricultural chemicals in livestock and marine products
20 min
80 mL
H2O
CO2
Bis(2-ethylhexyl) adipate
RI
Phthalic acid esters
PCBs
Chlordane, Toxaphene
10
20
min
Polychlorinated naphthalene
Polybrominated biphenylether
Aliphatic hydrocarbon
C19
C12
Methylanthracene
Anthracene
Column : Shodex CLNpak PAE-G AC
+ PAE-2000 AC
Eluent
: Acetone
Flow rate : 4.0mL/min
Detector : UV(213nm)
Data provided by Mr. Koichi Sasaki et al.,
Analytical Center, Kokan Keisoku Corporation.
Compound
Rt(min) Compound
Anthracene
Chrysene
Fluoranthene
Benzo[a]flucranthene
Benzo[b]flucranthene
Benzo[k]flucranthene
Pyrene
1-Hydroxypyrene
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
16.6
18.0
17.1
17.7
18.6
18.4
17.9
19.7
30
Rt(min)
Benzo[a]pyrene
Hydroxybenzo[a]pyrene
compound
Bisphenol A
1,3-Dinitronaphthalene
1-Nitropyrene
1,3-Dinitropyrene
1,6-Dinitropyrene
1,8-Dinitropyrene
19.9
23.0
16.1
16.2
18.6
18.6
18.9
18.7
Shodex CLNpak PAE-800 AC
Acetone
0.8mL/min
UV(210nm)
Room temp.
Data provided by Kazuichi Hayakawa Ph.D.,
Faculty of Pharmaceutical Sciences, Kanazawa University.
RI
4-Nonylphenol
DDT
RI
UV(330nm)
Bisphenol A
UV(330nm)
Anthracene
0
15
Column
:
Eluent
:
Flow rate
:
Detector
:
Column temp. :
20
25
30
35
min
Shodex CLNpak PAE-800 AC
Acetone
0.5mL/min
UV(330nm), RI
Room temp.
63
Sample Pretreatment
Columns
Pretreatment Columns for Column Switching
Method
Features
PK
Effective for both hydrophilic and hydrophobic substances
The high protein removal rate enables efficient pretreatment
News
Pretreatment Columns for Column Switching Method
GF-4A
No.9, 11, 22
Higher protein removal rate than PK columns
* GF-4A column removes proteins well but is not suitable for trapping hydrophilic substances.
Use PK columns for this purpose.
News
No.9, 11, 22
Cartridge columns and holder for column switching method
Product Code
Product Name
Particle Size
(µm)
Pore Size
(Å)
Column Size
(mm)
I.D. x Length
Shipping Solvent
Pcs/box
F8700000
MSpak PK-2A 2p
30
30
2.0 x 10
H2O
2
F8700012
MSpak PK-4A 2p
30
30
4.0 x 10
H2O
2
F8700001
MSpak HLD
−
−
(Holder for PK)
−
1
Base Material : Hydrophilic copolymers containing N-vinyl acetoamide
* PK series are cartridge columns and thus should be installed in a column holder "MSpak HLD" before use.
Column for column switching method
Product Code
Product Name
Particle Size
(µm)
Pore Size
(Å)
Column Size
(mm)
I.D. x Length
Shipping Solvent
F8700015
MSpak GF-4A
9
400
4.6 × 10
H2O
Base Material : Polyvinyl alcohol
64
*Contact Shodex or our distributors near you for customized columns.
500
Injector
Analytical column
Column
Protein removal
rate
PK-2A
99.62%
GF-2A*
99.80%
Company-A
97.68%
Detector
400
Residual BSA (μg)
Pretreatment column
300
200
Sample Pretreatment
Columns
Protein removal rate using pretreatment columns
System diagram for column switching
100
Pump 1
Eluent 1
Proteins etc.
in sample
PK-2A
(Pretreatment)
Eluent
: 10mM Ammonium acetate buffer(pH7.0)
Flow rate
: 0.5mL/min
Column temp. : Room temp.
GF-2A Company-A
(Analysis)
Column
: Shodex PROTEIN KW-604S**
Eluent
: 0.1% TFA in (H2O/CH3CN=50/50)
Flow rate
: 0.5mL/min
Detector
: UV(280nm)
*GF-2A: Custom-made column of
Column temp. : Room temp.
GF-4A (2.0mm I.D. x 10mm)
Switching time : 5min
**KW-604S is phase-out product.
Eluent 2
Recovery rate of medical compounds using PK-2A
Sample
Acetaminophen
Acetylpheneturide
Aconitine
Recovery
(%)
Recovery
(%)
Sample
115
Cloxazolam
91
92
Desipramine
109
110
Diazepam
Alprazolam
99
Diphenhydramine
Amitriptyline
93
Estazolam
Amobarbital
93
Ethenzamide
Barbital
94
Etizolam
Benzoylaconitine
96
Fludiazepam
Benzoylhypaconine
83
Biperiden
99
Sample
Recovery
(%)
Mianserin
92
Nimetazepam
90
97
Nitrazepam
97
93
Nortriptyline
86
101
Oxazepam
97
98
Oxazolam
99
Pentobarbital
93
97
Perfenazine
86
Flumazenil
93
Phenacetin
108
Flunitrazepam
97
Phenobarbital
96
105
Bromazepam
102
Flurazepam
106
Phenytoin
99
Bromocriptine
80
Glutethimide
93
Primidone
91
Bromperidol
89
Haloperidol
99
Promethazine
92
Bromvalerylurea
94
Haloxazolam
93
Propericiazine
90
97
1-Hydroxymethyltriazolam
90
Propranolol
97
4-Hydroxytriazolam
91
Secobarbital
97
Brotizolam
Caffeine
106
Carbamazepine
97
Hydroxyzine
99
Sildenafil citrate
95
Carpipramine
99
Hypaconitine
97
Thioridazine
97
Imipramine
97
Timiperone
88
Indomethacin
93
Triazolam
96
Levomepromazine
96
Trihexyphenidyl
91
Chlordiazepoxide
Chlormezanone
Chlorpheniramine
133
92
111
Chlorpromazine
77
Lofepramine
65
Trimethadione
137
Clocapramine
95
Maprotyline
90
Trimipramine
107
Clofedanol
91
Medazepam
91
Warfarin
81
Clomipramine
95
Mephobarbital
99
Zotepine
92
Clonazepam
96
Mesaconine
118
Clotiazepam
96
Metharbital
94
(Adsorption)
Eluent
: 10mM Ammonium acetate buffer (pH7.0)
Flow rate : 0.5mL/min
(Elution)
Eluent
:
Flow rate
:
Detector
:
Switching time :
Pretreatment Columns for Column Switching Method
0
Pump 2
10mM Ammonium acetate buffer (pH7.0)/CH 3CN
0.5mL/min
UV(220nm)
5min
65
Pharmaceuticals & Cosmetics
Application Fields
Column List for Pharmaceuticals and Cosmetics Analysis
Pharmaceuticals, Metabolites
Product Name
Pharmaceuticals and Cosmetics Analysis
Separation Mode
Page
ODP2 HP
RPC
6, 58
Asahipak ODP-40
RPC
8
Asahipak ODP-50
RPC
8, 58
Asahipak C8P-50
RPC
8
Asahipak C4P-50
RPC
8
RSpak DS series
RPC
10
RSpak DE series
RPC
10, 58
RSpak NN series
IEC+RPC
10, 58
Asahipak NH2P series
HILIC
12, 58
ODSpak F-411
RPC
14
Silica C18M
RPC
14
Silica C18P
RPC
14
LEX+SEC
16
RPC
56
USPpak MN-431
ET-RP1
[Substances in bio-fluid]
Product Name
Separation Mode
Page
ODP2 HP
RPC
6, 58
Asahipak GS-320 HQ
SEC+RPC
32
Asahipak GF-310 HQ
SEC+RPC
34
MSpak GF-310
SEC+RPC
34, 58
MSpak PK
SEC+RPC
64
Emulsifiers
Product Name
Separation Mode
Page
Asahipak GF-310 HQ
SEC+RPC
34
MSpak GF-310
SEC+RPC
34, 58
GPC KF-802
SEC
36
GPC KF-402HQ
SEC
42, 58
Preservatives
Separation Mode
Page
Asahipak ODP-40
Product Name
RPC
8
Asahipak ODP-50
RPC
8, 58
RSpak DS series
RPC
10
RSpak DE series
RPC
10, 58
ODSpak F-411
RPC
14
Silica C18M
RPC
14
Silica C18P
RPC
14
Optical active materials
Separation Mode
Page
ORpak CDBS-453
Product Name
CS
56
ORpak CRX-853
CS
56
Moisturizers
[Polyalcohols]
Product Name
Separation Mode
Page
RPC
10, 58
SUGAR SC1011
LEX+SEC
16
SUGAR SC1211
LEX+HILIC
16
OHpak SB-802.5 HQ
SEC
30
Asahipak GF-310 HQ
SEC
34
MSpak GF-310
SEC
34, 58
Separation Mode
Page
RSpak DE series
[Protein hydrolysates]
Product Name
Asahipak ODP-40
RPC
8
Asahipak ODP-50
RPC
8, 58
RSpak RP18-415
RPC
10
PROTEIN KW-802.5
SEC
28
KW402.5
SEC
28
Separation Mode
Page
SEC
30
[Mucopolysaccharides]
Product Name
OHpak SB-800 HQ series
66
Separation Mode
RPC
HILIC
NPC
LEX
IEX
IC
SEC
IEC
HIC
AFC
CS
: Reversed Phase Chromatography
: Hydrophilic Interaction Chromatography
: Normal Phase Chromatography
: Ligand Exchange Chromatography
: Ion Exclusion Chromatography
: Ion Chromatography
: Size Exclusion Chromatography
: Ion Exchange Chromatography
: Hydrophobic Interaction Chromatography
: Affinity Chromatography
: Chiral Separation Chromatography
Food
Nutritional ingredients
[Monosaccharides, Disaccharides, Sugar alcohols]
Product Name
Separation Mode
Asahipak NH2P series
HILIC
Page
12, 58
SUGAR SC1011
LEX+SEC
SUGAR SC1821
SUGAR SP0810
[Fatty acids]
Product Name
Separation Mode
Page
RPC
10
16
RSpak DE series
RPC
10, 58
LEX+SEC
16
Silica C18M
RPC
14
LEX+SEC
16
Silica C18P
RPC
14
SUGAR KS-801
LEX+SEC
16
Silica 5SIL
RPC
14
SUGAR KS-802
LEX+SEC
16
Asahipak GF-310 HQ
SEC
34
SUGAR SC1211
LEX+HILIC
16
MSpak GF-310
SEC
34, 58
SUGAR SZ5532
LEX+HILIC
16
GPC KF-802
SEC
36
RSpak DC-613
LEX+HILIC
16
GPC KF-402 HQ
SEC
42, 58
Separation Mode
Page
IEC+SEC
32
[Oligosaccharides]
[“Umami”, Nucleic acids]
Product Name
Separation Mode
Page
Asahipak NH2P series
HILIC
12, 58
SUGAR KS-802
SEC
16
RSpak DC-613
Product Name
Asahipak GS-320 HQ
[Amino acids]
LEX+HILIC
16
OHpak SB-802 HQ
SEC
30
OHpak SB-802.5 HQ
SEC
30
RSpak NN series
Asahipak GS-220 HQ
SEC
32
IC YS-50
[Low molecular weight water-soluble fiber]
Product Name
Separation Mode
Page
SEC
32
Asahipak GS-220 HQ
Product Name
CXpak P-421S
Separation Mode
Page
IEC+IEX+RPC
10, 58
IC
24
IEC
54
Separation Mode
Page
Food safety
[Food additives]
[Polysaccharides]
Product Name
Separation Mode
SUGAR KS-800 series
SEC
OHpak SB-800 HQ series
SEC
Page
16
RPC
8
30
Asahipak ODP-50
RPC
8, 58
RSpak DS series
RPC
10
RSpak DE series
RPC
10, 58
Asahipak NH2P series
HILIC
12, 58
ET-RP1
RPC
56
Separation Mode
Page
RPC
6, 58
RSpak DE series
RPC
10, 58
RSpak NN series
IEX+RPC
10, 58
RSpak KC-811
IEX+RPC
20
ODP2 HP
Product Name
Asahipak ODP-40
[Organic acids]
Product Name
Food Analysis
RSpak RP18 series
Application Fields
Column List for Food Analysis
[Pesticides]
Product Name
SUGAR SH1011
IEX+RPC
20
RSpak DE series
SUGAR SH1821
IEX+RPC
20
RSpak NN series
IC SI-90 4E
IC SI-90 4E
IC
22
IC SI-50 4E
IC
22
IC SI-35 4D
IC
22
IC SI-52 4E
IC
22
Separation Mode
Page
RPC
10, 58
IEC+RPC
10, 58
IC
22
Separation Mode
Page
RPC
14
[Mycotoxins]
Product Name
Silica C18M 4E
[Water-soluble vitamins]
Product Name
Separation Mode
Page
ODP2 HP
RPC
6, 58
Asahipak ODP-40
RPC
8
Asahipak ODP-50
RPC
8, 58
RSpak DE series
RPC
10, 58
RSpak NN series
IEC+RPC
10, 58
HILIC
12, 58
Separation Mode
Page
Asahipak NH2P series
[Fat-soluble vitamins]
Product Name
Asahipak ODP-40
RPC
8
Asahipak ODP-50
RPC
8, 58
Silica 5SIL
NPC
14
GPC KF-801
SEC
36
GPC KF-401HQ
SEC
42, 58
[Pretreatment of residual pesticides]
Product Name
Separation Mode
Page
CLNpak EV series
SEC
(GPC Clean-up)
62
Separation Mode
RPC
HILIC
NPC
LEX
IEX
IC
SEC
IEC
HIC
AFC
CS
: Reversed Phase Chromatography
: Hydrophilic Interaction Chromatography
: Normal Phase Chromatography
: Ligand Exchange Chromatography
: Ion Exclusion Chromatography
: Ion Chromatography
: Size Exclusion Chromatography
: Ion Exchange Chromatography
: Hydrophobic Interaction Chromatography
: Affinity Chromatography
: Chiral Separation Chromatography
67
Biotechnology
Application Fields
Column List for Biotechnology Analysis
Genomics
Proteomics
[Nucleobases, Nucleotides, Nucleosides]
Product Name
[Amino acids]
Biotechnology Analysis
Separation Mode
Page
RSpak DE series
RPC
10, 58
Asahipak ODP-50
RPC
8, 58
RSpak NN series
IEC+RPC
10, 58
RSpak NN series
IEC+IEX+RPC
10, 58
Asahipak GS-320 HQ
IEC+SEC
32
IC YS-50
IEC
24
IEC
52
CXpak P-421S
AXpak WA-624
Product Name
Asahipak GS-320 HQ
Page
IEC
54
IEC+SEC
32
Separation Mode
Page
[Oligo nucleic acids]
Product Name
RSpak DE series
Separation Mode
Page
[Peptides, Proteins]
RPC
10, 58
Product Name
IEC+SEC
32
Asahipak ODP-40
RPC
8
IEC DEAE3N-4T
IEC
52
Asahipak ODP-50
RPC
8, 58
Asahipak ES-502N 7C
IEC
52
Asahipak C8P-50
RPC
8
Asahipak C4P-50
RPC
8
RSpak RP18-415
RPC
10
PROTEIN KW-800 series
SEC
28
KW400 series
SEC
28
OHpak SB-800 HQ series
SEC
30
Asahipak GS-HQ series
SEC
32
Asahipak GF-HQ series
SEC
34
IEC QA-825
IEC
52
IEC DEAE-825
IEC
52
IEC DEAE3N-4T
IEC
52
PIKESS DEAE-2B
IEC
52
Asahipak ES-502N 7C
IEC
52
IEC SP-825
IEC
54
IEC SP-420N
IEC
54
PIKESS SP-2B
IEC
54
IEC CM-825
IEC
54
Asahipak ES-502C 7C
IEC
54
HIC PH-814
HIC
56
Asahipak GS-320 HQ
[DNA, RNA]
Product Name
OHpak SB-800 HQ series
Asahipak GF-HQ series
Separation Mode
Page
SEC
30
SEC
34
Hormones
[Amines]
Product Name
Separation Mode
Page
ODP2 HP
RPC
6, 58
Asahipak ODP-40
RPC
8
Asahipak ODP-50
RPC
8, 58
Asahipak C8P-50
RPC
8
Asahipak C4P-50
RPC
8
RSpak DS series
RPC
10
RSpak DE series
RPC
10, 58
Asahipak ES-502C 7C
IEC
54
Separation Mode
[Steroids]
Product Name
68
Separation Mode
Separation Mode
Page
Asahipak ODP-40
RPC
8
Asahipak ODP-50
RPC
8, 58
Asahipak NH2P series
HILIC
12, 58
OHpak SB-802.5 HQ
SEC
30
Asahipak GF-310 HQ
SEC
34
MSpak GF-310
SEC
34, 58
RPC
HILIC
NPC
LEX
IEX
IC
SEC
IEC
HIC
AFC
CS
: Reversed Phase Chromatography
: Hydrophilic Interaction Chromatography
: Normal Phase Chromatography
: Ligand Exchange Chromatography
: Ion Exclusion Chromatography
: Ion Chromatography
: Size Exclusion Chromatography
: Ion Exchange Chromatography
: Hydrophobic Interaction Chromatography
: Affinity Chromatography
: Chiral Separation Chromatography
Biotechnology
Glycomics
Lipids
[Glycoproteins]
Product Name
[Phospholipids]
Separation Mode
Page
Product Name
Separation Mode
Page
Silica 5SIL
NPC
14
Asahipak GF-310 HQ
SEC
34
RPC
8
Asahipak ODP-50
RPC
8, 58
Asahipak C8P-50
RPC
8
MSpak GF-310
SEC
34, 58
Asahipak C4P-50
RPC
8
GPC KF-802
SEC
36
RSpak RP18-415
RPC
10
GPC KF-402HQ
SEC
42
PROTEIN KW-800 series
SEC
28
KW400 series
SEC
28
OHpak SB-800 HQ series
SEC
30
Separation Mode
Page
Asahipak GS-HQ series
SEC
32
OHpak SB-805 HQ
SEC
30
Asahipak GF-HQ series
SEC
34
AFpak ADS-894
AFC
56
IEC QA-825
IEC
52
AFpak AHR-894
AFC
56
IEC DEAE-825
IEC
52
IEC DEAE3N-4T
IEC
52
PIKESS DEAE-2B
IEC
52
Asahipak ES-502N 7C
IEC
52
IEC SP-825
IEC
54
IEC SP-420N
IEC
54
PIKESS SP-2B
IEC
54
IEC CM-825
IEC
54
Asahipak ES-502C 7C
IEC
54
HIC PH-814
HIC
56
AFpak AWG-894
AFC
56
Product Name
Separation Mode
Page
Asahipak NH2P series
HILIC
12, 58
AFpak AWG-894
AFC
56
[Lipoproteins]
Product Name
Biotechnology Analysis
Asahipak ODP-40
Application Fields
Column List for Biotechnology Analysis
[Sugar chains]
[Monosaccharides, Disaccharides, Sugar alcohols]
Product Name
Separation Mode
Page
Asahipak NH2P series
HILIC
12, 58
SUGAR SC1011
LEX+SEC
16
SUGAR SC1821
LEX+SEC
16
SUGAR SP0810
LEX+SEC
16
SUGAR KS-801
LEX+SEC
16
SUGAR KS-802
LEX+SEC
16
SUGAR SC1211
LEX+HILIC
16
SUGAR SZ5532
LEX+HILIC
16
RSpak DC-613
LEX+HILIC
16
[Sialic acids, Uronic acids, Aldonic acids]
Product Name
Separation Mode
Page
SUGAR SH1011
IEX+SEC
20
SUGAR SH1821
IEX+SEC
20
Separation Mode
RPC
HILIC
NPC
LEX
IEX
IC
SEC
IEC
HIC
AFC
CS
: Reversed Phase Chromatography
: Hydrophilic Interaction Chromatography
: Normal Phase Chromatography
: Ligand Exchange Chromatography
: Ion Exclusion Chromatography
: Ion Chromatography
: Size Exclusion Chromatography
: Ion Exchange Chromatography
: Hydrophobic Interaction Chromatography
: Affinity Chromatography
: Chiral Separation Chromatography
69
Environment
Application Fields
Column List for Environmental Analysis
Environmental hormones
Water quality
[Anions]
Product Name
Separation Mode
Page
IC NI-424
IC
22
IC I-524A
IC
22
IC SI series
IC
22
Pretreatment of Phthalates, PCBs,
Benzo[a]pyrene
Product Name
CLNpak PAE series
Separation Mode
SEC
(GPC Clean-up)
Page
62
[Oxyhalides]
Environmental Analysis
Separation Mode
Page
IC SI-35 4D
Product Name
IC
22
IC SI-52 4E
IC
22
IEC
58
RSpak JJ-50 2D
[Cyanide, Cyanogen chloride]
Product Name
RSpak KC-811 6E
Separation Mode
Page
IEX
20
Separation Mode
Page
IC YS-50
IC
24
IC YK-421
IC
24
IC Y-521
IC
24
[Surfactants]
Product Name
[Monosaccharides, Oligosaccharides]
Product Name
Separation Mode
Page
Asahipak NH2P series
HILIC
12, 58
LEX+SEC
16
SUGAR SP0810
[Cations]
Product Name
Bioethanol
Separation Mode
Page
Asahipak ODP-50 4D
RPC
8
Silica C18M
RPC
14
Asahipak GF-310 HQ
SEC+RPC
34
MSpak GF-310
SEC+RPC
34, 58
Separation Mode
Page
IC
22
IEC
58
[Oligosaccharides, Alcohols, Furfural]
Separation Mode
Page
SUGAR KS-802
Product Name
LEX+SEC
16
SUGAR SC1821
LEX+SEC
16
[Saccharides, Organic acids, Alcohols, Furfural]
Separation Mode
Page
SUGAR SH1011
Product Name
IEX+RPC+SEC
20
SUGAR SH1821
IEX+RPC+SEC
20
[Hemicellulose, Cellulose]
Separation Mode
Page
GPC KD-800 series
Product Name
SEC
40
GPC LF-804
SEC
44
Separation Mode
Page
IC
24
[Perchloric acids]
Product Name
SI-90 4E
RSpak JJ-50 2D
Biodiesel
[Cations]
Product Name
[Pesticides]
Product Name
IC YS-50
Separation Mode
Page
RSpak DE series
RPC
10, 58
RSpak NN series
IEC+RPC
10, 58
IC SI-90 4E
IC
22
Soil
Page
IC NI-424
IC
22
IC I-524A
IC
22
IC SI series
IC
22
[Heavy metals]
IC T-521
Product Name
Page
SEC
34
Asahipak GF-310 HQ
SEC
34
MSpak GF-310
SEC
34, 58
Separation Mode
Page
RPC
10
Product Name
Separation Mode
Product Name
Separation Mode
Asahipak GF-210 HQ
[Fatty acid methyl esters]
[Anions]
Product Name
[Fatty acid glycerides]
Separation Mode
Page
IC
24
Separation Mode
Page
SEC
30
RSpak DS series
[Organic acids]
Separation Mode
Page
IC SI-35 4D
Product Name
IC
22
IC SI-52 4E
IC
22
[Humic substances]
Product Name
OHpak SB-805 HQ
[Organic arsenics]
Product Name
RSpak NN series
Separation Mode
Page
IEC+RPC
10, 58
[Pesticides]
Product Name
70
Separation Mode
Page
RSpak DE series
RPC
10, 58
RSpak NN series
IEC+RPC
10, 58
IC
22
IC SI-90 4E
Separation Mode
RPC
HILIC
NPC
LEX
IEX
IC
SEC
IEC
HIC
AFC
CS
: Reversed Phase Chromatography
: Hydrophilic Interaction Chromatography
: Normal Phase Chromatography
: Ligand Exchange Chromatography
: Ion Exclusion Chromatography
: Ion Chromatography
: Size Exclusion Chromatography
: Ion Exchange Chromatography
: Hydrophobic Interaction Chromatography
: Affinity Chromatography
: Chiral Separation Chromatography
No.
Packing Material
Recommended Column
Page
Octadecyl silane chemically bonded to porous or non-porous silica or ceramic
micro-particles, 1.5 to 10μm in diameter, or a monolithic rod
ODSpak F-411
14
14
14
L3
Porous silica particles, 1.5 to 10μm in diameter, or a monolithic silica ro
Silica 5SIL
14
L7
Octylsilane chemically bonded to totally or superficially porous silica particles, 1.5 to 10μm
in diameter, or a monolithic silica rod
Silica 5C8
14
L8
An essentially monomolecular layer of aminopropylsilane chemically bonded to totally
porous silica gel support, 1.5 to 10μm in diameter
Silica 5NH
14
L10
Nitrile groups chemically bonded to porous silica particles, 1.5 to 10μm in diameter
Silica 5CN
14
L11
Phenyl groups chemically bonded to porous silica particles, 1.5 to 10μm in diameter
Silica 5NPE
14
Silica C18P
20
20
20
RSpak KC-811
24
IC Y-521
16
SUGAR SC1011
16
SUGAR SC1821
16
SUGAR SC1211
16
USPpak MN-431
28
PROTEIN KW-800 series
28
KW400 series
36, 38, 40, 42,
GPC KF,K,KD,HFIP,LF,AT,UT series 44, 46, 48
SUGAR SH1011
L17
Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene
copolymer in the hydrogen form, 6 to 12μm in diameter
L19
Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene
copolymer in the calcium form, about 9μm in diameter
L20
Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5 to
10μm in diameter
L21
A rigid, spherical styrene-divinylbenzene copolymer, 3 to 30μm in diameter
SUGAR SH1821
RSpak DS-613
IC Y-521
10
10
10
54
16
16
16
16
16
16
16
16
20
20
20
24
IEC QA-825
52
RSpak DS-413
RSpak RP18-415
CXpak P-421S
SUGAR SP0810
SUGAR SC1011
SUGAR SC1821
SUGAR KS-800 series
L22
A cation-exchange resin made of porous polystyrene gel with sulfonic acid groups,
about 10μm in size
SUGAR SC1211
SUGAR SZ5532
USPpak MN-431
RSpak DC-613
SUGAR SH1011
SUGAR SH1821
RSpak KC-811
L23
An anion-exchange resin made of porous polymethacrylate or polyacrylate gel with
quartenary ammonium groups, 7-12μm in size
Packing having the capacity to separate compounds with a molecular weight range from
100-5000 (as determined by polyethylene oxide), applied to neutral, anionic, and cationic
water-soluble polymers. A polymethacrylate resin base, cross-linked with polyhydroxylated
ether (surface contained some residual carboxyl functional groups) was found suitable
OHpak SB-802 HQ
30
L25
OHpak SB-802.5 HQ
30
L26
Butyl silane chemically bonded to totally porous silica particles, 1.5 to 10μm in diameter
Silica 5C4
L33
Packing having the capacity to separate dextrans by molecular size over a range of 4,000
to 500,000Da. It is spherical, silica-based, and processed to provide pH stability
PROTEIN KW-800 series
KW400 series
14
28
28
L34
Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene
copolymer in the lead form, about 7 to 9μm in diameter
SUGAR SP0810
16
L37
Packing having the capacity to separate proteins by molecular size over a range of 2,000
to 40,000 Da. It is a polymethacrylate gel
OHpak SB-803 HQ
30
L38
A methacrylate-based size-exclusion packing for water-soluble samples
OHpak SB-800 HQ series
30
OHpak SB-800 HQ series
30
6, 58
10
L39
A hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin
L45
Beta cyclodextrin, R,S-hydroxypropyl ether derivative, bonded to porous silica particles,
5 to 10μm in diameter
L58
Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene
copolymer in the sodium form, about 6 to 30μm diameter
L59
Packing for the size-exclusion separations of proteins (separation by molecular weight)
over the range of 5 to 7000kDa. It is spherical (1.5 - 10μm), silica or hybrid packing with
a hydrophilic coating.
ODP2 HP
RSpak DM-614
L67
L71
Porous vinyl alcohol copolymer with a C18 alkyl group attached to the hydroxyl group of
the polymer, 2 to 10μm in diameter
A rigid, spherical polymetacrylate, 4 to 6μm in diameter
ORpak CDBS-453
56
CXpak P-421S
RSpak DC-613
54
16
16
PROTEIN KW-800 series
28
KW400 series
28
SUGAR KS-800 series
Asahipak ODP-40
Asahipak ODP-50
ET-RP1
RSpak DE-613
RSpak DE-413
RSpak DE-213
8
8, 58
56
10
10
10, 58
USP (Ver.35) Column List
L1
Silica C18M
Application fields
USP (Ver.35 US Pharmacopeia) Column List
71
Semi-micro and
Micro Columns
Semi-micro
Micro Columns
Semi-micro and Micro Columns
Polymer-based Packed Columns for Reversed Phase and Hydrophilic Interaction Chromatography (HILIC)
Asahipak ODP-40 semi-micro and micro type
RSpak DE-413 semi-micro and micro type
Base Material
: Polyvinyl alcohol
Functional Group : Octadecyl
Base Material : Polymethacrylate
Semi-micro
Micro Columns
I.D.
Polymer-based Packed Columns for Reversed Phase and Hydrophilic Interaction Chromatography (HILIC)
2.0mm
Length
(mm)
Product Name
I.D.
Product Code
250
ODP40-2E
F7838021
150
ODP40-2D
F7838022
Length
(mm)
Product Name
Product Code
250
DE413-2E
F7840121
150
DE413-2D
F7840122
2.0mm
50
ODP40-2B
F7838023
50
DE413-2B
F7840123
35
ODP40-2T
F7838024
35
DE413-2T
F7840124
250
ODP40-1E
F7838031
250
DE413-1E
F7840131
150
ODP40-1D
F7838032
150
DE413-1D
F7840132
1.0mm
1.0mm
50
ODP40-1B
F7838033
50
DE413-1B
F7840133
35
ODP40-1T
F7838034
35
DE413-1T
F7840134
250
ODP40-M5E
F7838051
150
DE413-M5D
F7840152
150
ODP40-M5D
F7838052
50
DE413-M5B
F7840153
50
ODP40-M5B
F7838053
35
DE413-M5T
F7840154
35
ODP40-M5T
F7838054
0.5mm
0.5mm
* See page 10 for RSpak DE-413 series.
* See page 78 for preparative columns.
* See page 8 for Asahipak ODP-40.
* See page 78 for preparative columns.
RSpak NN-414 semi-micro and micro type
Asahipak NH2P-40 semi-micro and micro type
Base Material
: Polyhydroxymethacrylate
Functional Group : Sulfo
Base Material
: Polyvinyl alcohol
Functional Group : Amino
I.D.
2.0mm
Length
(mm)
Product Name
I.D.
Product Code
150
NN414-2D
F7860122
50
NN414-2B
F7860123
Length
(mm)
Product Name
250
NH2P40-2E
150
NH2P40-2D
2.0mm
1.0mm
Product Code
Name Chenge
Refer to p.58
35
NN414-2T
F7860124
50
NH2P40-2B
150
NN414-1D
F7860132
35
NH2P40-2T
F7858024
50
NN414-1B
F7860133
250
NH2P40-1E
F7858031
35
NN414-1T
F7860134
150
NH2P40-1D
F7858032
1.0mm
0.5mm
150
NN414-M5D
F7860152
50
NH2P40-1B
F7858033
50
NN414-M5B
F7860153
35
NH2P40-1T
F7858034
35
NN414-M5T
F7860154
250
NH2P40-M5E
F7858051
150
NH2P40-M5D
F7858052
50
NH2P40-M5B
F7858053
35
NH2P40-M5T
F7858054
* See page 10 for RSpak NN-414.
0.5mm
* See page 12 for Asahipak NH2P-40 series.
* See page 79 for preparative columns.
72
*Contact Shodex or our distributors near you for customized columns.
Semi-micro
Micro Columns
Aqueous SEC (GFC) Column : Silica-based
KW402.5 semi-micro and micro type
KW403 semi-micro and micro type
Base Material : Silica
Base Material : Silica
I.D.
Length
(mm)
150
Product Name
KW402.5-4D
Product Code
F7781212
0.5mm
Semi-micro
Micro Columns
Product Name
Product Code
150
KW403-4D
F7781312
50
KW402.5-4B
F7781213
50
KW403-4B
F7781313
250
KW402.5-2E
F7781221
250
KW403-2E
F7781321
150
KW402.5-2D
F7781222
150
KW403-2D
F7781322
50
KW402.5-2B
F7781223
50
KW403-2B
F7781323
250
KW402.5-1E
F7781231
250
KW403-1E
F7781331
150
KW402.5-1D
F7781232
150
KW403-1D
F7781332
50
KW402.5-1B
F7781233
50
KW403-1B
F7781333
250
KW402.5-M5E
F7781251
250
KW403-M5E
F7781351
150
KW402.5-M5D
F7781252
150
KW403-M5D
F7781352
50
KW402.5-M5B
F7781253
50
KW403-M5B
F7781353
* See page 28 for KW402.5-4F.
* See page 81 for preparative columns.
2.0mm
1.0mm
0.5mm
* See page 28 for KW403-4F.
* See page 81 for preparative columns.
KW404 semi-micro and micro type
KW405 semi-micro and micro type
Base Material : Silica
Base Material : Silica
I.D.
Length
(mm)
Product Name
Product Code
150
KW404-4D
F7781412
4.6mm
2.0mm
1.0mm
0.5mm
I.D.
Length
(mm)
Product Name
Product Code
150
KW405-4D
F7781512
Aqueous SEC (GFC) Column : Silica-based
1.0mm
Length
(mm)
4.6mm
4.6mm
2.0mm
I.D.
Semi-micro and
Micro Columns
Semi-micro and Micro Columns
4.6mm
50
KW404-4B
F7781413
50
KW405-4B
F7781513
250
KW404-2E
F7781421
250
KW405-2E
F7781521
150
KW404-2D
F7781422
150
KW405-2D
F7781522
50
KW404-2B
F7781423
50
KW405-2B
F7781523
250
KW404-1E
F7781431
250
KW405-1E
F7781531
150
KW404-1D
F7781432
150
KW405-1D
F7781532
50
KW404-1B
F7781433
50
KW405-1B
F7781533
250
KW404-M5E
F7781451
250
KW405-M5E
F7781551
150
KW404-M5D
F7781452
150
KW405-M5D
F7781552
50
KW404-M5B
F7781453
50
KW405-M5B
F7781553
* See page 28 for KW404-4F.
* See page 81 for preparative columns.
2.0mm
1.0mm
0.5mm
* See page 28 for KW405-4F.
* See page 81 for preparative columns.
73
*Contact Shodex or our distributors near you for customized columns.
Semi-micro and
Micro Columns
Semi-micro
Micro Columns
Semi-micro
Micro Columns
Semi-micro and Micro Columns
Aqueous SEC (GFC) Columns : Polymer-based
OHpak SB-802 HQ semi-micro and micro type
OHpak SB-802.5 HQ semi-micro and micro type
Base Material : Polyhydroxymethacrylate
Base Material : Polyhydroxymethacrylate
I.D.
Aqueous SEC (GFC) Columns : Polymer-based
4.6mm
2.0mm
1.0mm
0.5mm
Length
(mm)
Product Name
250
SB802-4E
F7770111
150
SB802-4D
F7770112
50
SB802-4B
250
Length
(mm)
Product Name
Product Code
250
SB802.5-4E
F7770211
150
SB802.5-4D
F7770212
F7770113
50
SB802.5-4B
F7770213
SB802-2E
F7770121
250
SB802.5-2E
F7770221
150
SB802-2D
F7770122
150
SB802.5-2D
F7770222
50
SB802-2B
F7770123
50
SB802.5-2B
F7770223
250
SB802-1E
F7770131
250
SB802.5-1E
F7770231
150
SB802-1D
F7770132
150
SB802.5-1D
F7770232
50
SB802-1B
F7770133
50
SB802.5-1B
F7770233
250
SB802-M5E
F7770151
250
SB802.5-M5E
F7770251
150
SB802-M5D
F7770152
150
SB802.5-M5D
F7770252
50
SB802-M5B
F7770153
50
SB802.5-M5B
F7770253
* See page 30 for OHpak SB-802 HQ.
* See page 81 for preparative columns.
4.6mm
2.0mm
1.0mm
0.5mm
* See page 30 for OHpak SB-802.5 HQ.
* See page 81 for preparative columns.
OHpak SB-803 HQ semi-micro and micro type
OHpak SB-804 HQ semi-micro and micro type
Base Material : Polyhydroxymethacrylate
Base Material : Polyhydroxymethacrylate
I.D.
4.6mm
2.0mm
1.0mm
0.5mm
Length
(mm)
Product Name
I.D.
Product Code
250
SB803-4E
F7770311
150
SB803-4D
F7770312
50
SB803-4B
250
Length
(mm)
Product Name
Product Code
250
SB804-4E
F7770411
150
SB804-4D
F7770412
F7770313
50
SB804-4B
F7770413
SB803-2E
F7770321
250
SB804-2E
F7770421
150
SB803-2D
F7770322
150
SB804-2D
F7770422
4.6mm
2.0mm
50
SB803-2B
F7770323
50
SB804-2B
F7770423
250
SB803-1E
F7770331
250
SB804-1E
F7770431
150
SB803-1D
F7770332
150
SB804-1D
F7770432
50
SB803-1B
F7770333
50
SB804-1B
F7770433
250
SB803-M5E
F7770351
250
SB804-M5E
F7770451
150
SB803-M5D
F7770352
150
SB804-M5D
F7770452
50
SB803-M5B
F7770353
50
SB804-M5B
F7770453
1.0mm
0.5mm
* See page 30 for OHpak SB-804 HQ.
* See page 81 for preparative columns.
* See page 30 for OHpak SB-803 HQ.
* See page 81 for preparative columns.
OHpak SB-805 HQ semi-micro and micro type
OHpak SB-806 HQ semi-micro and micro type
Base Material : Polyhydroxymethacrylate
Base Material : Polyhydroxymethacrylate
I.D.
4.6mm
2.0mm
1.0mm
0.5mm
74
I.D.
Product Code
Length
(mm)
Product Name
I.D.
Product Code
Length
(mm)
Product Name
Product Code
250
SB806-4E
F7770611
150
SB806-4D
F7770612
F7770513
50
SB806-4B
F7770613
SB805-2E
F7770521
250
SB806-2E
F7770621
150
SB805-2D
F7770522
150
SB806-2D
F7770622
250
SB805-4E
F7770511
150
SB805-4D
F7770512
50
SB805-4B
250
4.6mm
2.0mm
50
SB805-2B
F7770523
50
SB806-2B
F7770623
250
SB805-1E
F7770531
250
SB806-1E
F7770631
150
SB805-1D
F7770532
150
SB806-1D
F7770632
50
SB805-1B
F7770533
50
SB806-1B
F7770633
250
SB805-M5E
F7770551
250
SB806-M5E
F7770651
150
SB805-M5D
F7770552
150
SB806-M5D
F7770652
50
SB805-M5B
F7770553
50
SB806-M5B
F7770653
* See page 30 for OHpak SB-805 HQ.
* See page 81 for preparative columns.
1.0mm
0.5mm
* See page 30 for OHpak SB-806 HQ.
* See page 81 for preparative columns.
*Contact Shodex or our distributors near you for customized columns.
Semi-micro
Micro Columns
Multimode Columns
Asahipak GS-220 HQ semi-micro and micro type
Asahipak GS-320 HQ semi-micro and micro type
Base Material : Polyvinyl alcohol
Base Material : Polyvinyl alcohol
I.D.
2.0mm
1.0mm
0.5mm
Product Name
Product Code
250
GS220A-4E
F7750211
150
GS220A-4D
F7750212
50
GS220A-4B
250
I.D.
Length
(mm)
Product Name
Product Code
250
GS320A-4E
F7750311
150
GS320A-4D
F7750312
F7750213
50
GS320A-4B
F7750313
GS220A-2E
F7750221
250
GS320A-2E
F7750321
150
GS220A-2D
F7750222
150
GS320A-2D
F7750322
50
GS220A-2B
F7750223
50
GS320A-2B
F7750323
250
GS220A-1E
F7750231
250
GS320A-1E
F7750331
150
GS220A-1D
F7750232
150
GS320A-1D
F7750332
50
GS220A-1B
F7750233
50
GS320A-1B
F7750333
250
GS220A-M5E
F7750251
250
GS320A-M5E
F7750351
150
GS220A-M5D
F7750252
150
GS320A-M5D
F7750352
50
GS220A-M5B
F7750253
50
GS320A-M5B
F7750353
4.6mm
2.0mm
1.0mm
0.5mm
* See page 32 for Asahipak GS-220 HQ.
* See page 82 for preparative columns.
* See page 32 for Asahipak GS-320 HQ.
* See page 82 for preparative columns.
Asahipak GS-520 HQ semi-micro and micro type
Asahipak GS-620 HQ semi-micro and micro type
Base Material : Polyvinyl alcohol
Base Material : Polyvinyl alcohol
I.D.
4.6mm
2.0mm
1.0mm
0.5mm
Length
(mm)
Product Name
Product Code
250
GS520A-4E
F7750511
150
GS520A-4D
F7750512
50
GS520A-4B
250
I.D.
Length
(mm)
Product Name
Product Code
250
GS620A-4E
F7750611
150
GS620A-4D
F7750612
F7750513
50
GS620A-4B
F7750613
GS520A-2E
F7750521
250
GS620A-2E
F7750621
150
GS520A-2D
F7750522
150
GS620A-2D
F7750622
50
GS520A-2B
F7750523
50
GS620A-2B
F7750623
250
GS520A-1E
F7750531
250
GS620A-1E
F7750631
150
GS520A-1D
F7750532
150
GS620A-1D
F7750632
50
GS520A-1B
F7750533
50
GS620A-1B
F7750633
250
GS520A-M5E
F7750551
250
GS620A-M5E
F7750651
150
GS520A-M5D
F7750552
150
GS620A-M5D
F7750652
50
GS520A-M5B
F7750553
50
GS620A-M5B
F7750653
* See page 32 for Asahipak GS-520 HQ.
* See page 82 for preparative columns.
Semi-micro
Micro Columns
Multimode Columns
4.6mm
Length
(mm)
Semi-micro and
Micro Columns
Semi-micro and Micro Columns
4.6mm
2.0mm
1.0mm
0.5mm
* See page 32 for Asahipak GS-620 HQ.
* See page 82 for preparative columns.
75
*Contact Shodex or our distributors near you for customized columns.
Semi-micro and
Micro Columns
Semi-micro
Micro Columns
Semi-micro
Micro Columns
Semi-micro and Micro Columns
Aqueous/ Organic SEC Columns
Asahipak GF-310 HQ semi-micro and micro type
Asahipak GF-510 HQ semi-micro and micro type
Base Material : Polyvinyl alcohol
Base Material : Polyvinyl alcohol
I.D.
Aqueous /Organic SEC Columns
4.6mm
2.0mm
1.0mm
0.5mm
Length
(mm)
Product Name
Product Code
250
GF310A-4E
F7760311
150
GF310A-4D
F7760312
50
GF310A-4B
250
I.D.
F7760511
150
GF510A-4D
F7760512
F7760313
50
GF510A-4B
F7760513
GF310A-2E
F7760321
250
GF510A-2E
F7760521
150
GF310A-2D
F7760322
150
GF510A-2D
F7760522
50
GF310A-2B
F7760323
50
GF510A-2B
F7760523
250
GF310A-1E
F7760331
250
GF510A-1E
F7760531
150
GF510A-1D
F7760532
GF510A-1B
F7760533
4.6mm
2.0mm
1.0mm
150
GF310A-1D
F7760332
50
GF310A-1B
F7760333
50
250
GF310A-M5E
F7760351
250
GF510A-M5E
F7760551
150
GF310A-M5D
F7760352
150
GF510A-M5D
F7760552
50
GF310A-M5B
F7760353
50
GF510A-M5B
F7760553
0.5mm
* See page 34 for Asahipak GF-510 HQ.
* See page 82 for preparative columns.
Asahipak GF-710 HQ semi-micro and micro type
2.0mm
1.0mm
0.5mm
Product Code
GF510A-4E
Base Material : Polyvinyl alcohol
4.6mm
Product Name
250
* See page 34 for Asahipak GF-310 HQ.
* See page 82 for preparative columns.
I.D.
Length
(mm)
Length
(mm)
Product Name
Product Code
250
GF710A-4E
F7760711
150
GF710A-4D
F7760712
50
GF710A-4B
F7760713
250
GF710A-2E
F7760721
150
GF710A-2D
F7760722
50
GF710A-2B
F7760723
250
GF710A-1E
F7760731
150
GF710A-1D
F7760732
50
GF710A-1B
F7760733
250
GF710A-M5E
F7760751
150
GF710A-M5D
F7760752
50
GF710A-M5B
F7760753
* See page 34 for Asahipak GF-710 HQ.
* See page 82 for preparative columns.
76
*Contact Shodex or our distributors near you for customized columns.
Semi-micro
Micro Columns
Columns for Cation Exchange Chromatography
Columns for Anion Exchange Chromatography
IEC QA-825 semi-micro and micro type
IEC SP-825 semi-micro and micro type
Base Material
: Polyhydroxymethacrylate
Functional Group : Quaternary ammonium
Base Material
: Polyhydroxymethacrylate
Functional Group : Sulfopropyl
I.D.
1.0mm
0.5mm
Product Name
Product Code
150
QA8-2D
F7940322
50
QA8-2B
F7940323
35
QA8-2T
150
I.D.
Length
(mm)
Product Name
Product Code
150
SP8-2D
F7940122
50
SP8-2B
F7940123
F7940324
35
SP8-2T
F7940124
QA8-1D
F7940332
150
SP8-1D
F7940132
50
QA8-1B
F7940333
50
SP8-1B
F7940133
35
QA8-1T
F7940334
35
SP8-1T
F7940134
150
QA8-M5D
F7940352
150
SP8-M5D
F7940152
50
QA8-M5B
F7940353
50
SP8-M5B
F7940153
35
QA8-M5T
F7940354
35
SP8-M5T
F7940154
2.0mm
1.0mm
0.5mm
* See page 52 for IEC QA-825.
* See page 80 for preparative columns.
* See page 54 for IEC SP-825.
* See page 81 for preparative columns.
IEC DEAE-825 semi-micro and micro type
IEC CM-825 semi-micro and micro type
Base Material
: Polyhydroxymethacrylate
Functional Group : Diethylaminoethyl
Base Material
: Polyhydroxymethacrylate
Functional Group : Carboxymethyl
I.D.
1.0mm
0.5mm
Product Name
Product Code
I.D.
Length
(mm)
Product Name
Product Code
150
CM8-2D
F7940222
50
CM8-2B
F7940223
F7940424
35
CM8-2T
F7940224
DEAE8-1D
F7940432
150
CM8-1D
F7940232
50
DEAE8-1B
F7940433
50
CM8-1B
F7940233
35
DEAE8-1T
F7940434
35
CM8-1T
F7940234
150
DEAE8-M5D
F7940452
150
CM8-M5D
F7940252
50
DEAE8-M5B
F7940453
50
CM8-M5B
F7940253
35
DEAE8-M5T
F7940454
35
CM8-M5T
F7940254
150
DEAE8-2D
F7940422
50
DEAE8-2B
F7940423
35
DEAE8-2T
150
2.0mm
1.0mm
0.5mm
* See page 54 for IEC CM-825.
* See page 81 for preparative columns.
* See page 52 for IEC DEAE-825.
* See page 80 for preparative columns.
Asahipak ES-502N semi-micro and micro type
Asahipak ES-502C semi-micro and micro type
Base Material
: Polyvinyl alcohol
Functional Group : Diethylaminoethyl
Base Material
: Polyvinyl alcohol
Functional Group : Carboxymethyl
I.D.
2.0mm
1.0mm
0.5mm
Length
(mm)
Product Name
Product Code
I.D.
Length
(mm)
Product Name
Product Code
150
CM9A-2D
F7960222
50
CM9A-2B
F7960223
F7960124
35
CM9A-2T
F7960224
DEAE9A-1D
F7960132
150
CM9A-1D
F7960232
50
DEAE9A-1B
F7960133
50
CM9A-1B
F7960233
35
DEAE9A-1T
F7960134
35
CM9A-1T
F7960234
150
DEAE9A-M5D
F7960152
150
CM9A-M5D
F7960252
50
DEAE9A-M5B
F7960153
50
CM9A-M5B
F7960253
35
DEAE9A-M5T
F7960154
35
CM9A-M5T
F7960254
150
DEAE9A-2D
F7960122
50
DEAE9A-2B
F7960123
35
DEAE9A-2T
150
* See page 52 for Asahipak ES-502N 7C.
* See page 80 for preparative columns.
2.0mm
1.0mm
0.5mm
* See page 54 for Asahipak ES-502C 7C.
* See page 81 for preparative columns.
*Contact Shodex or our distributors near you for customized columns.
Columns for Cation Exchange Chromatography
2.0mm
Length
(mm)
Semi-micro
Micro Columns
Columns for Anion Exchange Chromatography
2.0mm
Length
(mm)
Semi-micro and
Micro Columns
Semi-micro and Micro Columns
77
Preparative
Columns
Preparative
Columns
Preparative Columns (I.D. 10mm~50mm)
Columns for Polymer-based Reversed Phase Chromatography
Asahipak ODP-50 preparative type
Preparative
Columns
Product Code
Product Name
Plate Number
(TP/column)
Functional Group
Particle Size
(µm)
Column Size (mm)
I.D. x Length
5
10.0 × 250
Columns for Polymer-based Reversed Phase Chromatography
F6820001
Asahipak ODP-50 10E
≥ 10,000
Octadecyl
F6820035
Asahipak ODP-90 20F
≥ 9,000
Octadecyl
9
20.0 × 300
F6820019
Asahipak ODP-130 28F
≥ 9,000
Octadecyl
13
28.0 × 300
F6710004
Asahipak ODP-130G 7B
(guard column)
Octadecyl
13
7.5 × 50
F6714029
Asahipak ODP-130G 20C
(guard column)
Octadecyl
13
20.0 × 100
* See page 8 for Asahipak ODP-50.
* See page 72 for semi-micro columns and micro columns.
Base Material : Polyvinyl alcohol
Asahipak C8P-50 preparative type
Product Code
Product Name
Plate Number
(TP/column)
Functional Group
Particle Size
(µm)
Column Size (mm)
I.D. x Length
10.0 × 250
F6820003
Asahipak C8P-50 10E
≥ 8,000
Octyl
5
F6714004
Asahipak C8P-50G 7B
(guard column)
Octyl
5
7.5 × 50
Base Material : Polyvinyl alcohol
* See page 8 for Asahipak C8P-50.
Asahipak C4P-50 preparative type
Product Code
Product Name
Plate Number
(TP/column)
Functional Group
Particle Size
(µm)
Column Size (mm)
I.D. x Length
10.0 × 250
F6820005
Asahipak C4P-50 10E
≥ 7,000
Butyl
5
F6714005
Asahipak C4P-50G 7B
(guard column)
Butyl
5
7.5 × 50
Base Material : Polyvinyl alcohol
* See page 8 for Asahipak C4P-50.
RSpak DE-613 preparative type
Product Code
Product Name
F6513013
RSpak DE-2013
F6700190
RSpak DE-LG
F6513015
RSpak DE-5013
F6700191
RSpak DE-LLG
Plate Number
(TP/column)
Functional Group
Particle Size
(µm)
Column Size (mm)
I.D. x Length
≥ 10,000
−
12
20.0 × 300
(guard column)
−
12
8.0 × 50
−
12
50.0 × 300
−
12
20.0 × 100
−
(guard column)
* See page 10 for RSpak DE-613.
* See page 72 for semi-micro columns and micro columns.
Base Material : Polymethacrylate
RSpak DM-614 preparative type
Product Code
Product Name
F6514014
RSpak DM-2014
F6700404
RSpak DM-LG
F6514022
RSpak DM-5014
F6700162
RSpak DM-LLG
Plate Number
(TP/column)
Functional Group
Particle Size
(µm)
Column Size (mm)
I.D. x Length
≥ 5,000
−
12
20.0 × 300
(guard column)
−
12
8.0 × 50
−
12
50.0 × 300
12
20.0 × 100
−
(guard column)
* See page 10 for RSpak DM-614.
78
*Contact Shodex or our distributors near you for customized columns.
−
Base Material : Polyhydroxymethacrylate
Preparative
Columns
Columns for Polymer-based Hydrophilic Interaction Chromatography (HILIC)
Preparative
Columns
Preparative Columns
Asahipak NH2P-50 preparative type
Product Code
Product Name
Plate Number
(TP/column)
Functional Group
Particle Size
(µm)
Column Size (mm)
I.D. x Length
5
10.0 × 250
Asahipak NH2P-50 10E
≥ 10,000
Amino
F6830031
Asahipak NH2P-90 20F
≥ 10,000
Amino
9
20.0 × 300
F6830007
Asahipak NH2P-130 28F
≥ 1,000
Amino
13
28.0 × 300
F6710017
Asahipak NH2P-130G 7B
(guard column)
Amino
13
* See page 12 for Asahipak NH2P-50.
* See page 72 for semi-micro columns and micro columns.
7.5 × 50
Base Material : Polyvinyl alcohol
Columns for Silica-based Reversed Phase Chromatography
Silica C18M preparative type
Product Code
Product Name
Plate Number
(TP/column)
Functional Group
Particle Size
(µm)
Column Size (mm)
I.D. x Length
10.0 × 250
F7560040
Silica C18M 10E
≥ 16,000
Octadecyl
5
F7560041
Silica C18M 20E
≥ 16,000
Octadecyl
5
* See page 14 for Silica C18M.
20.0 × 250
Base Material : Silica
Silica 5C8 preparative type
Product Code
Product Name
Plate Number
(TP/column)
Functional Group
Particle Size
(µm)
Column Size (mm)
I.D. x Length
10.0 × 250
F7560062
Silica 5C8 10E
≥ 15,000
Octyl
5
F7560063
Silica 5C8 20E
≥ 15,000
Octyl
5
* See page 14 for Silica 5C8.
20.0 × 250
Base Material : Silica
Silica 5C4 preparative type
Product Code
Product Name
Plate Number
(TP/column)
Functional Group
Particle Size
(µm)
Column Size (mm)
I.D. x Length
10.0 × 250
F7560054
Silica 5C4 10E
≥ 15,000
Butyl
5
F7560055
Silica 5C4 20E
≥ 15,000
Butyl
5
* See page 14 for Silica 5C4.
20.0 × 250
Base Material : Silica
Columns for Silica-based Normal Phase and Hydrophilic Interaction Chromatography (HILIC)
Silica 5SIL preparative type [For normal phase chromatography]
Product Code
Product Name
Plate Number
(TP/column)
Functional Group
Particle Size
(µm)
Column Size (mm)
I.D. x Length
F7560050
Silica 5SIL 10E
≥ 15,000
−
5
10.0 × 250
F7560051
Silica 5SIL 20E
≥ 15,000
−
5
20.0 × 250
* See page 14 for Silica 5SIL.
Base Material : Silica
Silica 5NH preparative type [For normal phase and hydrophilic interaction chromatography (HILIC)]
Product Code
Product Name
Plate Number
(TP/column)
Functional Group
Particle Size
(µm)
Column Size (mm)
I.D. x Length
F7560060
Silica 5NH 10E
≥ 8,000
Aminopropyl
5
10.0 × 250
F7560061
Silica 5NH 20E
≥ 8,000
Aminopropyl
5
20.0 × 250
* See page 14 for Silica 5NH.
Columns for Polymer-based Hydrophilic Interaction Chromatography (HILIC) Columns for Silica-based Reversed Phase Chromatography
Columns for Silica-based Normal Phase and Hydrophilic Interaction Chromatography (HILIC)
F6830001
Preparative
Columns
Base Material : Silica
79
*Contact Shodex or our distributors near you for customized columns.
Preparative
Columns
Preparative
Columns
Preparative Columns
Columns for Ligand Exchange Chromatography
SUGAR KS-800 series preparative type
Preparative
Columns
Plate Number
(TP/column)
Columns for Ligand Exchange Chromatography
Columns for Anion Exchange Chromatography
Product Code
Product Name
F6502007
SUGAR KS-2001
≥ 7,000
F6502008
SUGAR KS-2002
≥ 7,000
F6502009
SUGAR KS-2003
≥ 8,000
F6502010
SUGAR KS-2004
≥ 6,000
F6502011
SUGAR KS-2005
≥ 6,000
F6502012
SUGAR KS-2006
≥ 6,000
F6700002
SUGAR KS-LG
Functional Group
(Counter Ion)
Sulfo (Na+)
Particle Size
(µm)
13
20.0 × 300
KS-801
Sulfo (Na+)
Sulfo (Na+)
13
20.0 × 300
KS-802
13
20.0 × 300
KS-803
Sulfo (Na+)
Sulfo (Na+)
18
20.0 × 300
KS-804
18
20.0 × 300
KS-805
Sulfo (Na+)
Sulfo (Na+)
18
20.0 × 300
KS-806
13
8.0 × 50
(guard column)
(guard column)
* See page 16 for SUGAR KS-800 series.
Column Size (mm)
I.D. x Length
Standard Column
Base Material : Styrene divinylbenzene copolymer
RSpak DC-613 preparative type
Product Code
Product Name
F6514013
RSpak DC-2013
F6700402
RSpak DC-LG
F6514021
RSpak DC-5013
F6700172
RSpak DC-LLG
Plate Number
(TP/column)
≥ 6,000
Functional Group
(Counter Ion)
Sulfo (Na+)
Particle Size
(µm)
Sulfo (Na+)
Sulfo (Na+)
10
8.0 × 50
10
50.0 × 300
Sulfo (Na+)
10
20.0 × 100
(guard column)
−
(guard column)
* See page 16 for RSpak DC-613.
10
Column Size (mm)
I.D. x Length
20.0 × 300
Base Material : Styrene divinylbenzene copolymer
Columns for Ion Exclusion Chromatography
Columns for Ion Exclusion Chromatography
RSpak KC-811 preparative type
Product Code
Product Name
F6505012
RSpak KC-2011
F6700010
RSpak KC-LG
Plate Number
(TP/column)
Functional Group
Particle Size
(µm)
≥ 8,000
Sulfo
13
20.0 × 300
(guard column)
Sulfo
13
8.0 × 50
* See page 20 for RSpak KC-811.
Column Size (mm)
I.D. x Length
Base Material : Styrene divinylbenzene copolymer
Columns for Anion Exchange Chromatography
IEC QA-825 preparative type
Product Code
Product Name
Functional Group
Particle Size
(µm)
Column Size (mm)
I.D. x Length
F6548000
IEC QA-2025
Quaternary ammonium
20
20.0 × 150
F6548050
IEC QA-5025
Quaternary ammonium
37
50.0 × 300
F6709602
IEC QA-LG
(guard column)
Quaternary ammonium
20
8.0 × 50
* See page 52 for IEC QA-825.
* See page 77 for semi-micro
columns and micro columns.
Base Material : Polyhydroxymethacrylate
IEC DEAE-825 preparative type
Product Code
Product Name
Functional Group
Particle Size
(µm)
Column Size (mm)
I.D. x Length
F6548001
IEC DEAE-2025
Diethylaminoethyl
20
20.0 × 150
F6598051
IEC DEAE-5025
Diethylaminoethyl
37
50.0 × 300
F6709603
IEC DEAE-LG
(guard column)
Diethylaminoethyl
20
8.0 × 50
* See page 52 for IEC DEAE-825.
* See page 77 for semi-micro
columns and micro columns.
Base Material : Polyhydroxymethacrylate
Asahipak ES-502N preparative type
Product Code
Product Name
Functional Group
Particle Size
(µm)
Column Size (mm)
I.D. x Length
F6840004
Asahipak ES-502N 20C
Diethylaminoethyl
13
20.0 × 100
F6710021
Asahipak GS-20G 7B
(guard column)
−
20
7.5 × 50
80
Base Material : Polyvinyl alcohol
*Contact Shodex or our distributors near you for customized columns.
* See page 52 for Asahipak ES-502N 7C.
* See page 77 for semi-micro
columns and micro columns.
Preparative
Columns
Columns for Cation Exchange Chromatography
Preparative
Columns
Preparative Columns
IEC SP-825 preparative type
Product Code
Product Name
Functional Group
Particle Size
(µm)
Column Size (mm)
I.D. x Length
IEC SP-2025
Sulfopropyl
20
20.0 × 150
F6548052
IEC SP-5025
Sulfopropyl
37
50.0 × 300
F6709604
IEC SP-LG
(guard column)
Sulfopropyl
20
8.0 × 50
Preparative
Columns
* See page 54 for IEC SP-825.
* See page 77 for semi-micro
columns and micro columns.
Base Material : Polyhydroxymethacrylate
IEC CM-825 preparative type
Product Code
Product Name
Functional Group
Particle Size
(µm)
Column Size (mm)
I.D. x Length
F6548003
IEC CM-2025
Carboxymethyl
20
20.0 × 150
F6548053
IEC CM-5025
Carboxymethyl
37
50.0 × 300
F6709605
IEC CM-LG
(guard column)
Carboxymethyl
20
8.0 × 50
* See page 54 for IEC CM-825.
* See page 77 for semi-micro
columns and micro columns.
Base Material : Polyhydroxymethacrylate
Asahipak ES-502C preparative type
Product Code
Product Name
F6840003
Asahipak ES-502C 20C
F6710021
Asahipak GS-20G 7B
(guard column)
Functional Group
Particle Size
(µm)
Column Size (mm)
I.D. x Length
Carboxymethyl
13
20.0 × 100
−
20
7.5 × 50
* See page 54 for Asahipak ES-502C 7C.
* See page 77 for semi-micro
columns and micro columns.
Base Material : Polyvinyl alcohol
PROTEIN KW-800 series preparative type
Column Size (mm)
I.D. x Length
Product Code
Product Name
Plate Number
(TP/column)
Particle Size
(µm)
F6505020
PROTEIN KW-2002.5
≥ 17,000
5
20.0 × 300
KW-802.5
F6505021
PROTEIN KW-2003
≥ 17,000
5
20.0 × 300
KW-803
F6505022
PROTEIN KW-2004
≥ 14,000
7
20.0 × 300
F6709556
PROTEIN KW-LG
(guard column)
7
8.0 × 50
* See page 28 for PROTEIN KW-800 series.
* See page 73 for semi-micro columns and micro columns.
Standard Column
KW-804
(guard column)
Base Material : Silica
Aqueous SEC (GFC) Columns : Polymer-based
Aqueous SEC (GFC) Columns : Silica-based
Aqueous SEC (GFC) Columns : Silica-based
Columns for Cation Exchange Chromatography
Aqueous SEC (GFC) Columns : Polymer-based
F6548002
OHpak SB-800 HQ series preparative type
Plate Number
(TP/column)
Column Size (mm)
I.D. x Length
20.0 × 300
F6516011
OHpak SB-2002
≥ 9,000
Particle Size
(µm)
15
F6516012
OHpak SB-2002.5
≥ 12,000
10
20.0 × 300
SB-802.5 HQ
F6516013
OHpak SB-2003
≥ 12,000
10
20.0 × 300
SB-803 HQ
F6516014
OHpak SB-2004
≥ 12,000
18
20.0 × 300
SB-804 HQ
F6516015
OHpak SB-2005
≥ 12,000
20
20.0 × 300
SB-805 HQ
F6516016
OHpak SB-2006
≥ 12,000
20
20.0 × 300
SB-806 HQ
F6516017
OHpak SB-2006M
≥ 12,000
20
20.0 × 300
SB-806M HQ
F6709555
OHpak SB-LG
(guard column)
18
8.0 × 50
(guard column)
Product Code
Product Name
* See page 30 for OHpak SB-800 HQ series.
* See page 74 for semi-micro columns and micro columns.
Standard Column
SB-802 HQ
Base Material : Polyhydroxymethacrylate
[Note] The maximum solvent tolerance of the SB-2000 series for methanol, acetonitrile, and DMF is 50%.
Solvent tolerance is different compared to the standard analytical SB-800 HQ columns.
81
*Contact Shodex or our distributors near you for customized columns.
Preparative
Columns
Preparative
Columns
Preparative Columns
Multimode Columns
Asahipak GS-HQ series preparative type
Product Code
Product Name
Preparative
Columns
Plate Number
(TP/column)
Particle Size
(µm)
Column Size (mm)
I.D. x Length
Standard Column
Multimode Columns
F6810017
Asahipak GS-220 20F
≥ 8,000
13
20.0 × 300
GS-220 HQ
F6810018
Asahipak GS-320 20F
≥ 8,000
13
20.0 × 300
GS-320 HQ
F6810019
Asahipak GS-520 20F
≥ 8,000
13
20.0 × 300
GS-520 HQ
F6810020
Asahipak GS-620 20F
≥ 8,000
13
20.0 × 300
GS-620 HQ
F6810034
Asahipak GS-220 20G
≥ 14,000
13
20.0 × 500
GS-220 HQ
F6810035
Asahipak GS-320 20G
≥ 14,000
13
20.0 × 500
GS-320 HQ
F6810036
Asahipak GS-520 20G
≥ 14,000
13
20.0 × 500
GS-520 HQ
F6810037
Asahipak GS-620 20G
≥ 14,000
13
20.0 × 500
GS-620 HQ
F6710021
Asahipak GS-20G 7B
(guard column)
20
7.5 × 50
(guard column)
Aqueous/Organic SEC Columns
* See page 32 for Asahipak GS-HQ series.
* See page 75 for semi-micro columns and micro columns.
Base Material : Polyvinyl alcohol
Aqueous/ Organic SEC Columns
Asahipak GF-HQ series preparative type
Product Code
Product Name
Plate Number
(TP/column)
Particle Size
(µm)
Column Size (mm)
I.D. x Length
Standard Column
Organic SEC (GPC) Columns
F6810030
Asahipak GS-310 20F
≥ 8,000
13
20.0 × 300
GF-310 HQ
F6810031
Asahipak GS-510 20F
≥ 8,000
13
20.0 × 300
GF-510 HQ
F6810032
Asahipak GS-710 20F
≥ 8,000
13
20.0 × 300
GF-710 HQ
F6810033
Asahipak GSM-700 20F
≥ 8,000
13
20.0 × 300
GF-7M HQ
F6810038
Asahipak GS-310 20G
≥ 14,000
13
20.0 × 500
GF-310 HQ
F6810039
Asahipak GS-510 20G
≥ 14,000
13
20.0 × 500
GF-510 HQ
F6810040
Asahipak GS-710 20G
≥ 14,000
13
20.0 × 500
GF-710 HQ
F6810041
Asahipak GSM-700 20G
≥ 14,000
13
20.0 × 500
GF-7M HQ
F6710020
Asahipak GS-10G 7B
(guard column)
20
7.5 × 50
(guard column)
* See page 34 for Asahipak GF-HQ series.
* See page 76 for semi-micro columns and micro columns.
Base Material : Polyvinyl alcohol
(Note) Usable solvents for GS-710 20F and 20G are water and methanol.
Solvent tolerance is different compared to standard analytical GF-710 HQ columns.
Selection of GSM-700 20F or 20G is recommended in case of use of other solvents for scale up testing with GF-710 HQ.
Organic SEC (GPC) Columns
GPC KF-800 series preparative type
Product Code
82
Product Name
Plate Number
(TP/column)
Particle Size
(µm)
Column Size (mm)
I.D. x Length
Standard Column
F6102401
GPC KF-2001
≥ 18,000
6
20.0 × 300
KF-801
F6102402
GPC KF-2002
≥ 18,000
6
20.0 × 300
KF-802
F6102425
GPC KF-2002.5
≥ 18,000
6
20.0 × 300
KF-802.5
F6102403
GPC KF-2003
≥ 18,000
6
20.0 × 300
KF-803
F6102404
GPC KF-2004
≥ 14,000
7
20.0 × 300
KF-804
F6102405
GPC KF-2005
≥ 10,000
10
20.0 × 300
KF-805
F6102406
GPC KF-2006
≥ 10,000
10
20.0 × 300
KF-806
F6102409
GPC KF-2006M
≥ 10,000
10
20.0 × 300
KF-806M
F6700406
GPC KF-LG
(guard column)
15
8.0 × 50
(guard column)
* See page 36 for GPC KF-800 series.
*Contact Shodex or our distributors near you for customized columns.
Base Material : Styrene divinylbenzene copolymer
Preparative
Columns
Organic SEC (GPC) Columns
Preparative
Columns
Preparative Columns
GPC K-800 series preparative type
Product Code
Product Name
Plate Number
(TP/column)
Particle Size
(µm)
Column Size (mm)
I.D. x Length
Standard Column
Preparative
Columns
GPC K-2001
≥ 18,000
6
20.0 × 300
K-801
F6102312
GPC K-2002
≥ 18,000
6
20.0 × 300
K-802
F6102315
GPC K-2002.5
≥ 18,000
6
20.0 × 300
K-802.5
F6102303
GPC K-2003
≥ 18,000
6
20.0 × 300
K-803
F6102304
GPC K-2004
≥ 14,000
7
20.0 × 300
K-804
F6102305
GPC K-2005
≥ 10,000
10
20.0 × 300
K-805
F6102306
GPC K-2006
≥ 10,000
10
20.0 × 300
K-806
F6102309
GPC K-2006M
≥ 10,000
10
20.0 × 300
K-806M
F6700407
GPC K-LG
(guard column)
15
8.0 × 50
* See page 38 for GPC K-800 series.
(guard column)
Base Material : Styrene divinylbenzene copolymer
Organic SEC (GPC) Columns
GPC K-800 series preparative type
Product Code
Product Name
Plate Number
(TP/column)
Particle Size
(µm)
Column Size (mm)
I.D. x Length
Standard Column
F6102001
GPC H-2001
≥ 13,000
15
20.0 × 500
K-801
F6102002
GPC H-2002
≥ 13,000
15
20.0 × 500
K-802
F6102025
GPC H-2002.5
≥ 13,000
15
20.0 × 500
K-802.5
F6102003
GPC H-2003
≥ 13,000
15
20.0 × 500
K-803
F6102004
GPC H-2004
≥ 13,000
15
20.0 × 500
K-804
F6102005
GPC H-2005
≥ 13,000
15
20.0 × 500
K-805
F6102006
GPC H-2006
≥ 13,000
15
20.0 × 500
K-806
F6102009
GPC H-2006M
≥ 12,000
15
20.0 × 500
K-806M
F6700310
GPC H-G
(guard column)
15
8.0 × 50
(guard column)
Preparative Columns [Custom-made]
Preparative Columns [Custom-made]
Organic SEC (GPC) Columns
F6102301
Base Material : Styrene divinylbenzene copolymer
* See page 38 for GPC K-800 series.
GPC KF-800 series preparative type
Product Code
Product Name
Particle Size
(µm)
Column Size (mm)
I.D. x Length
Standard Column
F6108010
GPC KF-5001
15
50.0 × 300
KF-801
F6108020
GPC KF-5002
15
50.0 × 300
KF-802
F6108025
GPC KF-5002.5
15
50.0 × 300
KF-802.5
F6108030
GPC KF-5003
15
50.0 × 300
KF-803
F6108040
GPC KF-5004
15
50.0 × 300
KF-804
F6700408
GPC KF-LLG
15
20.0 × 100
(guard column)
Base Material : Styrene divinylbenzene copolymer
* See page 36 for GPC KF-800 series.
GPC K-800 series preparative type
Product Code
Product Name
Particle Size
(µm)
Column Size (mm)
I.D. x Length
Standard Column
F6109010
GPC K-5001
15
50.0 × 300
K-801
F6109020
GPC K-5002
15
50.0 × 300
K-802
F6109025
GPC K-5002.5
15
50.0 × 300
K-802.5
F6109030
GPC K-5003
15
50.0 × 300
K-803
F6109040
GPC K-5004
15
50.0 × 300
F6700409
GPC K-LLG
15
20.0 × 100
* See page 38 for GPC K-800 series.
K-804
(guard column)
Base Material : Styrene divinylbenzene copolymer
*Contact Shodex or our distributors near you for customized columns.
83
Information
Phase-out Products
Phase-out products remain available to customers in need of them for the purpose of quality control, etc.
For more information please contact Shodex or our distributors near you.
Please note that phase-out products may go out of production in the future.
Polymer-based Packed Columns for
Reversed Phase Chromatography
Columns for Rare Earth Metal Ions
Phase-out Products
Product Name
Column Size
(mm)
I.D. x Length
Product Name
Column Size
(mm)
I.D. x Length
RSpak RP18-613
6.0 × 150
IC R-621
6.0 × 50
RSpak RP18-413
4.6 × 150
IC R-G
4.6 × 10
RSpak DE-413S
4.6 × 50
RSpak GOLF-413
4.6 × 150
Aqueous SEC Semi-micro Columns
Product Name
Column for Oxyhalides
Product Name
Column Size
(mm)
I.D. x Length
IC SI-91 4C
4.0 × 100
Column Size
(mm)
I.D. x Length
SB401-4E
4.6 × 250
SB402.5-4E
4.6 × 250
SB403-4E
4.6 × 250
SB404-4E
4.6 × 250
SB400G-4A
4.6 × 10
Multimode Columns
Columns for Anions in Wine
Column Size
(mm)
I.D. x Length
Product Name
Column Size
(mm)
I.D. x Length
MSpak GS-320 4B
4.6 × 50
WINE VH-anion 4D
4.6 × 150
MSpak GS-320 4D
4.6 × 150
WINE VH-anionG 4A
4.6 × 10
MSpak GS-320 2D
2.0 × 150
Product Name
84
Information
Columns for Affinity Chromatography
Columns for Chiral Separation
Column Size
(mm)
I.D. x Length
Product Name
Column Size
(mm)
I.D. x Length
AFpak AAB-894
8.0 × 50
ORpak CDA-453 HQ
4.6 × 150
AFpak AAF-894
8.0 × 50
ORpak CDB-453 HQ
4.6 × 150
AFpak AAM-894
8.0 × 50
ORpak CDC-453 HQ
4.6 × 150
AFpak AAP-894
8.0 × 50
ORpak CD-G
4.6 × 10
AFpak AAV-894
8.0 × 50
AFpak ABA-894
8.0 × 50
AFpak ABT-894
8.0 × 50
AFpak ACA-894
8.0 × 50
AFpak ACB-894
8.0 × 50
AFpak AED-894
8.0 × 50
AFpak AGA-894
8.0 × 50
AFpak AGE-894
8.0 × 50
AFpak AGT-894
8.0 × 50
AFpak AIA-894
8.0 × 50
AFpak ALC-894
8.0 × 50
AFpak ALS-894
8.0 × 50
AFpak ANA-894
8.0 × 50
AFpak AOV-894
8.0 × 50
AFpak APB-894
8.0 × 50
AFpak APD-894
8.0 × 50
AFpak APE-894
8.0 × 50
AFpak APH-894
8.0 × 50
AFpak APR-894
8.0 × 50
AFpak AST-894
8.0 × 50
AFpak AGO-494
4.6 × 10
Phase-out Products
Product Name
Column for Column Switching Method
Product Name
Column Size
(mm)
I.D. x Length
PROTEIN KW-604S
6.0 × 50
85
Information
Column Cleaning Procedures
Changes in peak shape, elution time, or elevated column pressure may be resolved by cleaning the column. This
section describes general indications of column deterioration and column cleaning procedures.
For details of column cleaning procedures, refer to each column’s specific operation manual.
Typical indicators of column deterioration possibility
1. Elevated column pressure
Column Cleaning Procedures
2. Abnormal peak shapes (broadening, leading, or tailing) and split peaks
3. Change in retention time
4. Unstable baseline
Selection guide to the cleaning solvent
Solvents capable of dissolving the adsorbed substances
Solvents with high eluting power (variable depending on separation mode)
*Use the solvent specified in the operation manual.
Standard cleaning procedures
For an efficient cleaning, reverse the direction and reduce the flow rate to 1/3 of the regular flow.
Columns for
reversed phase
chromatography
Clean the columns with solvent containing higher concentration of organic solvent such as
methanol, acetonitrile, or THF.
(In case of using buffer as a mobile phase, miscibility of the buffer solution and the organic
solvents need to be checked)
Columns for
sugar analysis
chromatography
[Ligand exchange columns (SUGAR series)]
• In case of counter ion detachment
Flush or inject solvent containing the salt corresponding to the modified counter-ligand.
[Polymer-base amino columns (NH2P series)]
• In cases where an acidic substance has been bound to the amino functional group
Flush with solvents in the following sequence: water, 0.1M perchloric acid (aq.), water,
0.1M NaOH (aq.), water, and mobile phase.
Columns for
aqueous SEC (GFC)
chromatography
• In cases where an ionic substance has been adsorbed
Use a solvent with higher salt concentration or solvent with different pH from the mobile phase.
• In cases where a hydrophobic substance has been adsorbed
Use a solvent containing organic solvent.
(In case of using buffer as a mobile phase, miscibility of the buffer solution and the organic
solvents need to be checked)
Columns for
ion exchange
chromatography
• In cases where an ionic substance has been adsorbed
Use a solvent with higher salt concentration or solvent with different pH from the mobile phase.
• In cases where a hydrophobic substance has been adsorbed
Use a solvent containing organic solvent.
(In case of using buffer as a mobile phase, miscibility of the buffer solution and the organic
solvents need to be checked)
• In cases where protein have been adsorbed
Inject 1-2 mL of 0.1 M NaOH (aq.) or 30% (v/v) acetic acid (aq.) several times.
Columns for
hydrophobic interaction
chromatography
• In cases where protein have been adsorbed
Inject 1-2 mL of 0.1 M NaOH (aq.) or 30% (v/v) acetic acid (aq.) several times.
*The required volume of the cleaning solvent is 5-10 times the column volume.
*Avoid pressure elevation during the cleaning.
*The cleaning is limited and does not guarantee the full regeneration of the column to its original condition.
For your information
One typical cause of the column pressure elevation is the clogging of solid substances at the inlet filter of the column.
In this case, reverse the direction and reduce the flow to 1/3 of the regular flow rate. This may remove the solid
substance causing the elevated pressure.
86
*Use the solvent specified in the operation manual.
For the best performance of the column, please follow the instructions given below.
Information
General Precautions for Column Handling
Column mounting
• Attach the column in the direction as indicated by arrow marked on the column. Gradually increase the flow rate of
the solvent introduced to the column.
• When heating the column, be sure to pump the eluent at a low flow rate until the specified temperature is reached,
and then gradually increase the flow rate up to the requirement after the column has been heated sufficiently.
Column dismounting
• If the column is heated, turn off the heater while keeping the flow rate at 1/3 of the regular flow.
• Turn off the pump when the column is cooled to room temperature.
• Remove the column from the system and securely tighten the end caps.
General Precautions for Column Handling
• Before mounting the column, replace the eluent within all the HPLC system with the mobile phase used for the
analysis. *If the mobile phase of the choice is not miscible with the eluent already in the system, use solvent that is
miscible with both solvents first to clean the system. *Buffer or salt solution may precipitate when mixed with
organic solvent of different concentrations.
Column storage
• For long-term storage, replace the solvent with shipping solvent and securely tighten the end caps.
• Store the column in a location with stable temperature.
• For long-term storage of SEC columns, immersion method is recommended.
*Please refer to the immersion method on the operation manual.
Other
• Avoid physical shock on the column. Be cautious not to drop the column from a high position.
• Do not bend the column.
• Avoid opening the column’s end-fitting, it can cause alteration of column’s performance.
* Read the operation manual before using the column.
87
Information
Column Trouble Shooting
Common causes for abnormal chromatograms
Unstable baseline values
Peak tailing
······Main cause
······Solution
NO
Column Trouble Shooting
Selection of
column and/
or eluent
appropriate
YES
Peak split,
shoulders
Peak
leading
NO
Broad peak
Problem solved
by reducing
injection volume
YES
······Frequent problem
Strong sample retention
Change column and/or eluent
Void existing at top of column
Replace column
Adsorption of impurities in
column
· Wash column
· Check column performance
· Replace column
Channeling in column
Replace column
Leading caused by sample
properties
(ionic and micellar properties)
Change column and/or eluent
· Adsorption of impurities in
column
· Deterioration of column
· Wash column
· Check column performance
· Replace column
Sample overload
Reduce injection volume
Prepare sample again
Contamination
Wash flow line of HPLC
Ghost peak
Impurities existing in eluent
Replace eluent
System trouble
Check system
· Eluent not flowing
· Injector at wrong position (load/inject)
· Detector deactivated
No peak
Sample adsorption in column
Repeat sample injection
· Column equilibration insufficient
Pressure elevation
Pump
C
Column
······Main cause
Detector
······Solution
······Frequent problem
YES
Pressure still high
after washing
NO
· Change eluent composition
· Wash system before analysis
Salting out
YES
NO
Pressure still high when
C is disconnected
B
Injector
START
Salt precipitated is
observed in eluent
A
NO
Detector piping
blocked
· Clean up detector
· Replace piping
YES
Pressure still high when N O
B is disconnected
YES
Column
blocked
Pressure under
maximum column
pressure
NO
Deterioration
of column
· Check column performance
· Replace column
YES
YES
Screen eluent and sample
through 0.45 μm filter
Pressure still high when N O
A is disconnected
Injector blocked
NO
Column end-fitting
blocked
Replace column
· Clean up injector
· Replace injector
YES
NO
Used line filter
Pump piping blocked
· Replace filter in pump
· Clean up pump
· Replace pump piping
YES
Line filter blocked
88
· Wash line filter
· Replace line filter
Common causes for abnormal chromatograms
Information
HPLC System Trouble Shooting
Unstable baseline values
······Main cause
······Solution
······Frequent problem
START
NO
Malfunction of check valve
· Replace check valve
· Clean up check valve
Temperature fluctuation
· Warm up detector sufficiently
· Shield detector from wind
· Check column oven
· Check detector temperature control
Deterioration of detector lamp
Replace lamp
Insufficient grounding
Establish sufficient grounding
Malfunction of recorder
Repair recorder
Bubbles in flow cell
Wash inside of detector
Contamination of flow cell
Wash inside of detector
Insufficient eluent replacement
Wash flow line of HPLC
YES
YES
Baseline stable when
pump stopped
NO
Monotonic drift
YES
NO
HPLC System Trouble Shooting
Column Pressure
normal
NO
Leakage observed
(with different solvent, salt or others)
YES
Contamination of column
· Wash column
· Check column performance
· Replace column
Pulsation of pump
Use damper
Deterioration of eluent
· Use eluent containing stabilizer
(for THF, use THF containing BHT)
Flow rate fluctuation
Tighten connections
Noise
······Main cause
······Solution
······Frequent problem
START
Baseline stable when
pump stopped
Insufficient grounding
Establish sufficient grounding
Deterioration of detector lamp
Replace detector lamp
Malfunction of recorder
Repair recorder
Insufficient degassing eluent
Use Shodex DEGAS
NO
Irregular noise
YES
NO
Shodex DEGAS used
Spike noise
YES
OK
YES
Spike noise found
from begining
YES
Air in column
Problem solved by applying back
pressure to column(*)
(*) Do not apply back pressure to detector
Column temperature exceeding eluent boiling point
NO
NO
· Check column performance
· Replace column
Reduce temperature of column
oven and/or post-column reaction
coil
NO
Noise shape leading
Contamination of flow cell
Wash flow cell
YES
Bubbles in flow cell
Repair detector
89
Index
Index by Product Name
Columns are listed in alphabetical order under the product name excluding series name.
[Series name]
AFpak
Asahipak
AXpak
CLNpak
CXpak
EP
GPC
HIC
IC
IEC
MSpak
ODSpak
OHpak
ORpak
PIKESS
PROTEIN
RSpak
Silicapak
STANDARD
STD
SUGAR
USPpak
WINE
Silica
Index by Product Name
A
AAB-894
AAF-894
AAM-894
AAP-894
AAV-894
ABA-894
ABT-894
ACA-894
ACB-894
ACH-494
ADS-894
AED-894
AGA-894
AGE-894
AGO-494
AGT-894
AHR-894
AIA-894
ALC-894
ALS-894
ANA-894
AOV-894
APA-894
APB-894
APD-894
APG-894
APH-894
APR-894
AST-894
AT-806MS, AT-G
AWG-894
D
85
85
85
85
85
85
85
85
85
56
56
85
85
85
85
85
56
85
85
85
85
85
56
85
85
56
85
85
85
46
56
C
C18M
C18P
C4P-50, C4P-50G 4A
5C4
5C8
C8P-50, C8P-50G 4A
CDA-453 HQ
CDB-453 HQ
CDBS-453
CDC-453 HQ
CD-G
CM-825
5CN
CRX-853, CRX-G
90
14
14
8
14
14
8
85
85
56
85
85
54
14
56
DC-613, DC-G
DE, DE-G, DE-SG
DE-413S
DEAE-2B
DEAE3N-4T
DEAE-825
DM-614, DM-G
DS-413, DS-613, DS-G
16
10, 58
84
52
52
52
10
10
E
E-411
ET-RP1
ES-502C 7C
ES-502N 7C
EV, EV-G
14
56
54
52
62
F
F-411, F-511, F-411/S
FL-1, FL-1 filter
FL-2, FL-2 filter
14
22, 24
22, 24
G
GF-1G 7B
GF-210 HQ
GF-310 HQ
GF-310
GF-4A
GF-510 HQ
GF-710 HQ
GF-7M HQ
GOLF-413
GS-220 HQ
GS-2G 7B
GS-320 HQ
GS-320
GS-520 HQ
GS-620 HQ
GS-620 7G-P
34
34
34
34, 58
64
34
34
34
84
32
32
32
84
32
32
32
H
HFIP-603~607, HFIP-606M, HFIP-G
HFIP-803~807, HFIP-806M, HFIP-LG
HLD
HT-803~807, HT-806M, HT-G
48
48
64
46
Index
I
I-524A, IA-G
Q
22
38
38, 40
20
40
42, 58
42
36, 40
36
36, 42, 58
16
16
28
85
28
L
LF-404, LF-604, LF-804, LF-G
44
M
M-75
MN-431
51
16
14
12, 58
12, 58
12
22
10, 58
14
6, 58
8
8, 58
P
P-421S, P-G
P-82
P
PAE, PAE-G
PH-814
PK
5PYE 4D
S
SB401-4E~404-4E, SB400G-4A
SB-802 H~Q806 HQ, SB-806M HQ, SB-G
SB-807 HQ, SB-807G
SC1011, SC1011-7F, SC1821, SC-LG
SC1211, SC-G
SH1011, SH1821, SH-G
SH-75
SI-35 4D
SI-50 4E
SI-52 4E
SI-90 4E
SI-91 4C
SI-50G, SI-90G, SI-92G, SI-95G
5SIL
SL-105
SM-105
SP0810, SP-G
SP-2B
SP-420N
SP-825
SZ5532, SZ-G
T-521, T-G
84
30
30
16
16
20
51
22
22
22
22
84
22
14
51
51
16
54
54
54
16
24
U
UT-802.5, UT-806M, UT-807, UT-G
46
V
VH-anion 4D, VH-anionG 4A
O
ODP2 HP, ODP2 HPG
ODP-40
ODP-50, ODP-50G
84
84
10
T
N
5NH
NH2P-40
NH2P-50, NH2P-50G
NH2P-LF
NI-424, NI-G
NN, NN-G
5NPE 4D
R-621, R-G
RP18-413, RP18-613
RP18-415, RP18-G
Index by Product Name
10, 58
K
K-801~807, K-803L~807L, K-806M, K-G
K-800D
KC-811, KC-G, KC-LG
KD-801~807, KD-806M, KD-G
KF-401HQ~404HQ, KF-405LHQ, 406LHQ
KF-601~607, KF-606M
KF-800D
KF-801~807, KF-803L~807L, KF-806M
KF-G
KS-801~806, KS-G
KS-807, KS-807G
KW402.5-4F~405-4F, KW400G-4A
KW-604S
KW-802.5~804, KW-G
52
R
J
JJ-50
QA-825
84
W
WA-624, WA-G
52
Y
54
51
51
62
56
64
14
Y-521, Y-G
YK-421, YK-G
YS-50, YS-G
24
24
24
* Semi-micro and micro columns and preparative columns are not included.
91
Technical Information
Technical Notebook, Chromato News
92
Technical Notebook, Chromato News
Note
book
Technical Notebook
No.
Title
No.
Title
1
Shodex GPC LF series columns
Linear calibration curves over a wide molecular weight range
5
Shodex KW400 series columns
High performance and downsized column for protein analysis
2
Shodex NH2P-50 series columns
Analysis of saccharides in food industry
6
Shodex ODP2 HP series columns
Better retention of highly polar substances
3
Food Analysis with Shodex columns
Saccharides, organic acids, vitamins, fatty acids and amino acids
7
Protein & peptide with Shodex columns
News
Chromato News
No.
Title
Column
1
High performance reversed phase column; polymer-based column Asahipak ODP-40
ODP-40 series
2
Direct analysis of medical compounds in serum without sample pretreatment
GF-310 series, GS-320 series
3
Semi-micro GPC columns with high resolution
KF-400HQ series
4
Linear calibration curves by GPC LF-804
LF-804
6
Food additives
SP0810, SC1011, NH2P-50 4E, KC-811
7
Polymer-based column with multimode of reversed phase and ionic interaction
NN series, JJ-50
8
Ultra high molecular weight and water-soluble polymer with SUGAR KS-807
KS-807, KS-800 series
9
Direct analysis of proteins and peptides in surfactants using surfactant removal
column MSpak PK series
PK series, GF-4A
10
Direct analysis of additives in polymer with SEC/MS
KF-400HQ series
11
Concentration of the hydrophilic medical compounds existing with proteins
PK series
12
Column selection for proteins and peptides analysis
SEC, Reversed phase, Ion exchange, Affinity columns
13
Analysis of hyaluronic acid with ultra high molecular weight in serum
KS-807, GS-620HQ
14
Water-soluble vitamins
NH2P-50 4E, DE-413
17
Saccharides and sugar alcohols with Asahipak NH2P-50 column
NH2P-50 series
22
Online removal of surfactants in protein analysis
PK-2A, KW-604S
23
Various application with RSpak DE series; high pH, 0% organic eluent
DE series
24
Analysis of amines and amides with RSpak DE series
DE-613, DE-413, ACH-494
25
Simultaneous analysis of monosaccharide and organic acid with SUGAR SH1011
SH1011
29
Water-soluble ultra high molecular compounds with OHpak SB-807HQ
SB-807HQ, SB-806HQ
30
New reversed phase column ODP2 HP
ODP2 HP series
31
New protein analysis column KW400
KW400 series
32
High sensitive analysis of pyridylaminated saccharides
NH2P-50
33
New rapid cation exchange column; DEAE3N-4T with non-porous gel
DEAE3N-4T
35
Shellfish poisoning and pesticides
DE413-2B, ODP-50 4E
36
Suitable column for LC/MS application; ODP2 HP
ODP2 HP
40
Analysis of sugars in bioethanol production
SH1821, KS-802, LF-804
41
High temperature reversed phase column in high temperature for rapid analysis
ET-RP1 4D
42
Ion exchange columns for UHPLC
PIKESS series
43
Saccharides analysis with H2O eluent
SP0810, SC1011, KS-801, SH1011
44
High resolution separation by NH2P-40 3E
NH2P-40 3E
Technical Support
Tel: +49 (0) 89 9399 6237
E-mail: info@shodex.de
Order
www.shodex.de
Tel: +49 (0) 89 9399 6234
E-mail: order@shodex.de
Showa Denko Europe GmbH
Konrad-Zuse-Platz 4
81829 Munich
Germany
Please contact a Shodex support office
Support office
Europe
Africa
Middle East
Russia
Showa Denko Europe GmbH
Konrad-Zuse-Platz 4, D-81829 Munich, Germany
TEL : +49 (0)89 93 99 62-37 FAX : +49 (0)89 93 99 62-50
E-mail : info@shodex.de
www.shodex.de
North America
Latin America
Showa Denko America, Inc.
420 Lexington Avenue, Suite 2335, New York, NY 10170, USA
TEL : +1 212 370 0033 FAX : +1 212 370 4566
E-mail : support@shodex.net
www.shodex.net
Southeast Asia
India
Taiwan
Oceania
Showa Denko K.K.
23F Muza Kawasaki Central Tower,
1310, Omiya-cho, Saiwai-ku, Kawasaki, Kanagawa 212-0014, Japan
TEL : +81 (0)44 520 1380 FAX : +81 (0)44 520 1383
E-mail : Sdk_Shodex@sdk.co.jp
www.shodex.com
China
Hong Kong
Macau
Shodex China Co., Ltd.
18F, Wang Wang Building, No.211 Shi Men Yi Road, Jing An,
Shanghai, 200041, P.R.China
TEL : +86 (0)21 6217-6111 FAX : +86 (0)21 6217-9879
E-mail : sales@shodexchina.com
www.shodex.com/index_ch.html
Korea
Shoko Korea Co., Ltd.
#322, Chungjeong Rizion, 465, Chungjeongno 3-ga,
Seodaemun-gu, Seoul, 120-013, Korea
TEL : +82 (0)2 784 5111 FAX : +82 (0)2 784 5125
E-mail : shoko.korea@shokokorea.com
www.shodex.com/index_kr.html
www.shodex.de
Manufactured by
SHOWA DENKO K.K.
Shodex (Separation & HPLC) Group
23F Muza Kawasaki Central Tower,
1310, Omiya-cho, Saiwai-ku, Kawasaki, Kanagawa 212-0014, Japan
TEL : +81-(0)44-520-1380 FAX : +81-(0)44-520-1383
CAT.NO.01E.
15.JAN.13