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ESSENTAIL TECHNIQUES IN MOLECULAR BIOLOGY
ASSIGNMENT # 1
MSDS (MATERIAL SAFETY DATA SHEET)
Name: Saad bin Habib (FA19-BSO-064)
Semester VI (Bioscience)
Instructor: Sir Afraz Ahmad
Date of submission: 23rd February 2022
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What is MSDS?
A MSDS or SDS document is a standardized data sheet which contains occupational safety
and health data regarding a chemical. The manufacturers of such chemicals are mandated by
the International Hazard Communication Standard (HCS) to provide a proper SDS to the
chemical handlers, for its safe application. It provides the user with the information about the
safe use, emergency and first aid procedure about the certain chemical. This type of
information is usually like the chemical properties (flammability, cohesiveness), health and
environmental hazards (irritant, toxic) of the chemical. [1]
Hazardous Pictogram:
These are symbols used to alert and inform about the potential hazardous effect of a chemical
or its products, to people or environment. These pictograms are represented in a shape of a
diamond, with red borders and white background. One or more of these can be found on the
packaging or label of chemicals. [2,3]
Represents corrosive
effect on skin and
other surfaces.
Must not be directly
touched. Shown by
a symbol
representing
corrosion.
Represents
hazardous effect to
the environment.
Such chemicals
must not be
disposed directly
into the
environment.
Represents
potential Explosive
properties of
chemicals. Must
avoid shacking,
mixing, heating or
dispersing the
chemical.
Represents
flammable in
liquid, gas or solid
state. Shown by a
symbol of a
flame. Must not
be kept away
from fire source.
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Chemically oxidizing
in nature. These are
potential firehazards, and must be
kept away from fire
and oxygen source.
Represents
compressed gases,
mixture of gases,
dissolved or
liquified gases
present under
pressure.
Represent acute
Toxic nature of
chemical. Shown
by skull and bone.
Must not be
tasted or applied
on skin.
Can cause chronic
health effects.
Unlike acute toxic,
these develop
slowly over time
and deteriorate
health.
Examples of Chemicals:
1. Diethyl ether ≥99.7% stabilised, AnalaR NORMAPUR® ACS, Reag. Ph. Eur.
analytical reagent:
Table:[4]
PROPERTY
VALUE
Boiling point:
35 Celsius
Melting point:
-123 Celsius
Flammability:
Extremely flammable in liquid and vapour
Coerciveness:
Non-corrosive to skin
Explosive property
Not Applicable
Colour:
Colourless
Carcinogenic:
No
Chemical stability:
Stable at room temperature
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Hazards:[4]
Category 1, flammable liquid. Category 4, Acute oral toxicity.
Extremely flammable liquid and vapour. Harmful if swallowed. May cause
drowsiness or dizziness, and may form explosive peroxides. Repeated exposure may
cause skin dryness or cracking. No evidence for being carcinogenic.
2. Potassium iodide:
Table:[5]
PROPERTY
VALUE
Boiling point:
1.33 Celsius
Melting point:
680 Celsius
Flammability:
Non-flammable
Coerciveness:
Yes
Colour:
Off-white
Vapour pressure:
ca.1 hPa at 745 °C
Chemical stability:
Stable at room temperature
Hazards: [5]
The substance can cause damage to organs through prolonged or repeated
exposure if swallowed. Potential carcinogenic at high doses. The substance/mixture is
however, not considered very persistent, bio accumulative or toxic at level of 0.1% or
higher.
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3. Methylene blue (C.I.52015) (manufacturer: Merck):
Table:[6]
PROPERTY
VALUE
Boiling point:
-
Melting point:
180 Celsius
Flammability:
flammable in liquid and vapour
Coerciveness:
Non-corrosive
Volatility:
Non-volatile
Colour:
Dark blue
Vapour pressure:
-
Chemical stability:
Stable at room temperature
Hazards: [6]
The substance is harmful if swallowed. Don`t eat or drink. Don`t let products enter drains.
Thoroughly wash hands and apparatus after use. Potential carcinogenic.
4. N, N'-Methylenebisacrylamide:
Table:[7]
PROPERTY
VALUE
Boiling point:
333.8 Celsius
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Melting point:
173.7 – 185.9 Celsius
Flammability:
Non-flammable, but can cause explosion in
dust form
Coerciveness:
-
Volatility:
-
Colour:
White
Vapour pressure:
< 0,01 hPa
Chemical stability:
Stable at room temperature
Hazards: [7]
The substance is toxic if swallowed. It may cause genetic defects. Proper storage
necessary. Thoroughly wash skin after use.
5. Phenolphthalein:
Table:[8]
PROPERTY
VALUE
Boiling point:
82 Celsius
Melting point:
-88 Celsius
Flammability:
Flammability
Coerciveness:
-
Volatility:
-
Colour:
Colourless
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Vapour pressure:
40mmHg
Chemical stability:
Stable at room temperature
Hazards: [8]
Carcinogenicity Category 2, suspected of causing cancer. Combustible in dust form,
hazard not contributing to classification.
6. Calcium Chloride (Dihydrate) Extra Pure:
Table:[9]
PROPERTY
VALUE
Boiling point:
Equal to or greater than 1600
Melting point:
176 Celsius
Flammability:
Non-flammable
Coerciveness:
Non coerciveness
Volatility:
-
Colour:
White powder
Vapour pressure:
0.01 hPa at 20 °C
Chemical stability:
Stable at room temperature
Hazards:[9]
Can causes serious eye irritation. In which case, rinse with water and expose to fresh
air.
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References:
1. Canadian centre for occupational health and safety, “WHMIS 1988 - Material Safety
Data Sheets (MSDSs): General”
https://www.ccohs.ca/oshanswers/legisl/msdss.html#:~:text=A%20Material%20Safet
y%20Data%20Sheet%20(MSDS)%20is%20a%20document%20that,safely%20with%
20the%20chemical%20product.&text=The%20MSDS%20contains%20much%20mor
e%20information%20about%20the%20material%20than%20the%20label.
2. Health and safety executive, “Hazard pictograms (symbols)”
https://www.hse.gov.uk/chemical-classification/labelling-packaging/hazard-symbolshazard-pictograms.htm
3. Canadian Centre for Occupational Health and Safety, “Health and safety teaching
tools, pictograms”, date of last modification: 2018-03-21
https://www.ccohs.ca/teach_tools/chem_hazards/symbols.html#:~:text=The%20picto
gram%20for%20oxidizing%20products,oxidizing%20liquids%20and%20oxidizing%
20solids.
4. Diethyl ether ≥99.7% stabilised, AnalaR NORMAPUR® ACS, Reag. Ph. Eur.
analytical reagent, Related information, SDS, by VWR, Revision date: 21.10.2019
https://uk.vwr.com/store/product/730521/diethyl-ether-99-7-stabilised-analarnormapur-acs-reag-ph-eur-analytical-reagent
5. Potassium iodide for analysis EMSURE® ISO,Reag. Ph Eur, SDS, by SUPLEOC,
Revision Date 27.03.2021
https://www.merckmillipore.com/INTL/en/product/msds/MDA_CHEM-105043
6. Methylene blue (C.I.52015), SDS, by SIGMA-ALDRICH, Revision Date 01.12.2021
https://www.merckmillipore.com/INTL/en/product/msds/MDA_CHEM-115943
7. N, N'-Methylenebisacrylamide for electrophoresis, special grade for molecular
biology, SDS, by SIGMA-ALDRICH, Revision Date 12.10.2021
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https://www.merckmillipore.com/INTL/en/product/msds/MDA_CHEM101546?Origin=PDP
8. Phenolphthalein, ACS, SDS, by LAB CHEM, Revision date: 03/05/2020,
http://www.labchem.com/tools/msds/msds/LC18198.pdf
9. CALCIUM CHLORIDE (DIHYDRATE) EXTRA PURE, MSDS, by LOBACHEMICA
https://www.lobachemie.com/lab-chemical-msds/MSDS-CALCIUM-CHLORIDEDIHYDRATE-CASNO-10035-04-02465-EN.aspx