dehydrating agents __________ cannot be used in processors or with isopropanol incomplete dehydration results in clearing agents must be miscible with ______ and _______. the dye-check solution of eosin can only be added to ________, not isopropanol a reagent that CANNOT be used for dehydrating tissues is: one disadvantage using dioxane for processing is paraffin used for embedding is ___ degrees celsius. Drierite (dehydratant) alcohol, acetone, dioxane, tetrahydrofuran (last two are universal solvents) dehydratant and paraffin mushy soft sections benzene (clearnat) ethanol (dehydratant) 53 degrees celsius it is TOXIC one xylene disadvantage is overheating of paraffin used for embedding may cause routine use paraffin melting point should be one advantage of paraffin use is which is not a clearing agent? chloroform/ethanol/dioxane/xylene when cutting a block, if the tissue seems hard and brittle... when used for clearing, cedar wood oil must be followed by SOFT paraffin is most useful when _______ sections are desired. cracking of the block it is likely to harden tissue serial sections are easy to obtain 55 to 58 degrees C overheated paraffin ethanol (dehydratant) thick xylene (clearant) paraffin is cooled rapidly as possible after embedding to prevent denatured alcohol dehydration and clearing steps can be omitted when METHYL ALCOHOL ETHYL ALCOHOL Dioxane Acetone ISOPROPANOL ethanol + methanol or ethanol + isopropanol (dehydratant) formation of large crystals POISONOUS dehydratant, NOT USED ALONE PEL=200 / not for skin; breaks down to formaldehyde; causes blindness and death using water-soluble wax (embedding medium) a UNIVERSAL SOLVENT which is faster, less shrinkage than ethanol, toxic, rarely used / PEL-OSHA=100 /PELNIOSH=1 a dehydratant that CANNOT BE USED IN PLACE OF ETHANOL WHEN STAINING! PEL=400 BEST DEHYDRATANT!!! PEL=1,000 / 60-65-95-95-absolute (dehydratant) PEL-OSHA=1,000 / PEL-NIOSH=250 / (rapid, cheap, but absorbs water from the air) USED IN TOUCH PREPS OR FAST FIXES. CAN ALSO BE USED LIKE A UNIVERSAL SOLVENT. BUTYL ALCOHOL PEL=100 / used as clearing agent in most labs Tertiary Butanol Toluene Tetrahydrofuran Benzene CHLOROFORM PEL=50 XYLENE SUBSTITUTES ARE xylene (clearant) 4 PLANTS AND ANIMAL MATERIAL! PEL=100 (dehydratant) BEST HYDROCARBON CLEARING AGENT, PEL=50, STEL=150 /not as hard as xylene (dehydratant)odorous, expensive, solidifies at room temperature,MIXES WITH WATER, ETHANOL, XYLENE / PEL=100 fast, does not over-harden, WILL HARDEN MUSCLE, TENDON, UTERUS more than TOLUENE // PEL=10 a universal solvent that is miscible in lower alcohols, water, xylene, toluene, benzene, chloroform, acetone,melted parrafin. less toxic than dioxane. PEL=200 / offensive odor, causes conjunctivitis/ BEST UNIVERSAL SOLVENT ALIPHATIC HYDROCARBONS AND LIMONENE chloroform is carcinogenic water soluble waxes 5% aqueous formic acid will a nitrocellular compound used for embedding (i. Parloidion). storage blocks held in 80% alcohol. hardened with chloroform. mixed with ether and absolute alcohol to prepare. celloidin MUST BE great for undecalcified bone, kidney, bone marrow, lymph node biopsies ethylene glycol monoethyl ether best processing for enzyme demonstration sections processed by _______ method show less shrinkage than when processed by paraffin. decalcify most bones in 3 to 5 days CARBOWAX: dehydrating and clearing unnecessary. Infiltrates tissue directly from fixative.BAD: floating out occurs dehydrated celloidin a dehydratant that retains tissues for extended periods of time. more than ethanol or 70% alcohol GMA Glycol Methacrylate , acrylic resin which is miscible with water. celloidin frozen sections ion exchange resin & electrolytic methods of decalcification are considered these agents EDTA)bind certain metals 30% sucrose acid methods chelating agents best way to get frozen sections from formalin fixed, unprocessed tissue, by infiltrating in this