LIPID PROFILE CLINICAL LABORATORY TESTING
ROUTINELY REPORTED
 High density lipoprotein (HDL-C) goood
cholesterol
 Low density lipoprotein (LDL-C)
 Triglyceride
 Total Cholesterol
*Very Low density Liporpotein
LDL – Computed/ Calculated and not
measured
Entire – LDL VLDL
Between LDL and VLDL, the answer will be LDL
since it is routinely reported
Since we compute the value VLDL from
triglyceride
FORMULA OF LDL
LDL-C = T Cholesterol – HDL – VLDL
2 METHODS
Friedewald Method
3 fromulas
o VLDL (mmol/L) = if TAG: >400mg/dl
TAG x 0.16
o VLDL (mmol/L) = if TAG: < = 400
mg/dl
TAG / 5.0
o VLDL (mg/dl) = if it expressed in SI
units
TAG / 2.175
EXAMPLE:
EXAMPLE 1
TAG= 205 mg/dl
Total Cholesterol= 250 mg/dl
HDL= 50 mg/dl
Solution:
VLDL
LDL
VLDL (mg/dl)
= TAG / 5
= 205 mg/dl / 5
= 41 mg/dl
LDL
= T Chole – hdl – VLDL
= 250 mg/ dl – 50 mg/dl – 41 mg/ dl
= 159 mg/dl
EXAMPLE 2
HDL = 1.5mmol/ L
Total Cholesterol = 6.0 mmol
TAG= 2.0 mmol/ L
VLDL
=TAG/ 2.175
= 2.0 / 2.175
= 0.9195402299 / 0.92 mmol/L
LDL
= Chole – hdl – VLDL
= 6.0 mmol/L – 1.5mmol/L – 0.92 mmol/ L
= 3.8 mmol/ L
 you cannot compute if your varaibles do
not have the same unit of measure
De Long Method (indirect Method)
2 FORMULAS:
o
VLDL (mmol / L) = TAG / 2.825
o
VLDL (mg/dL) = TAG/ 6.5
Creatinine Clearance Test
Used to measure glomerular nutrition rate.
GFR through creatinie clearance testmeasures the efficiency of kidney’s filtration
capability.
CREATINE
- test being measured
- It is produced when ther is muscle activity
-
Creatinine Phosphokinase will add to ADO to
become ATP( muscle energy requirement)
For example, you have 3 bottles: What will you
do?
- MIX ALL 3 bottles and aliquote (small
fraction of sample)
How much of sample will be aliquoted?: 50 mL
Processing:
Centrifuged urine
Use the filtrate
1. aliquted urine
2. Serum sample
– Red top tube or yellow top tube
3. urine Volume: Ex: 3L
Formula
CrClT= UV/ P x 1.773/ A
= (Urine creatinine) (Urine Volume ml/min) /
plasma creatinen x 1.73/ body surface area
-
Creatine
Is the waste product
It Will now then be introduced to the
circulation
In the kidney, water will be removed from
creatine=therfore forming creatinine
99% of body’s creatinine is filtered out
0.6- 1.3 mg/ dl = normally found in serum
sample
Creatinine Clearance test
Specimen type:
24- hour urine sample collection
1st Urine: 6am-7am: DISCARD first urine
2nd Urine: 9:15 am = COLLECT
Last collection: 7:03 am the next day
How to collect the sample:
If body surface area is not given = consider it
as 1.73
URINE VOLUME: 3L
UV=3l/ mins. In 24h period
Given:
Urine crea= 2015 mg/dL
P/S Crea= 1.0 mg/dl
\
ASSIGNMENT
]
REFERENCE RANGE
Men: 107-139 ml/min
Women: 87-107 ml/ min
EXAMPLE