ENZYME WEBQUEST
WEBSITE #1:
http://www.northland.cc.mn.us/biology/Biology1111/animations/enzyme.html
Choose ENZYMES: The BASICS
1. SLIDE ONE: Enzymes are ________________that serve as __________________.
They _________________up or _______________ ______________reactions, but
ALWAYS remain _________________.
2. SLIDE TWO: What is an ACTIVE SITE?
3. SLIDE THREE: Each ______________acts on a specific_____________.
4. What is a SUBSTRATE?
5. DRAW an ENZYME and SUBSTRATE below. Make each a DIFFERENT COLOR and label
each.
6. SLIDE FOUR: What is the INDUCED FIT?
7. SLIDE FIVE: YOU JUST DRANK A GLASS OF MILK! DRAW THE CATALYTIC CYCLE OF
LACTOSE BELOW! LABELING THE SUBSTRATE, SUBSTRATE ENZYME COMPLEX, THE
ACTIVE SITE, AND THE PRODUCTS. MAKING EACH A DIFFERENT COLOR!
8. Someone who is “LACTOSE INTOLERANT”…is lacking which
enzyme?____________________
WEBSITE #2: http://www.phschool.com/science/biology_place/labbench/lab2/intro.html
Enzyme Catalysis
Introduction
9. ______________________ catalyze reactions by lowering the
10. _____________________ ___________________ necessary for a reaction to occur.
Key Concepts
11. _________________ catalyze reactions by lowering the _______________
_________________ necessary for a reaction to occur. The molecule that an enzyme acts
on is called the _________________. In an enzyme-mediated reaction,
_________________ molecules are changed, and _________________ is formed. The
_________________molecule is _________________ after the reaction, and it can
continue
_____________________________________________________________________
______________________________________________________________.
12. Each _________________ is _________________for the _________________ it will
catalyze. In this laboratory,
Enzyme = catalase, found in your liver
Substrate = hydrogen peroxide (H2O2)
Products = water and oxygen
13.
DRAW THE REACTION THAT TOOK
PLACE ON THE LEFT USING YOUR OWN
SHAPES,
LABELING
THE
SUBSTRATE,
ENZYME, ACTIVE SITE ON THE ENZYME,
ENZYME-SUBSTRATE COMPLEX, AND THE
PRODUCTS.
Enzyme Structure
14. Enzymes are globular _____________________. Their folded conformation creates an
area known as the _________________ _______________. The nature and arrangement
of __________________ ____________________ in the __________________
__________________ make it
specific__________________________________________________________.
15. DRAW A MODEL OF AN ENZYME, ACTIVE SITE, AND SUBSTRATE BELOW:
Binding Specificity
16. Even when different _________________ molecules are present, only those that have
the _________________ _______________ _____________________ to the
_________________ _______________ are able to bind with the enzyme's
_______________ ________________.
Induced Fit
17. Observe the INDUCED FIT ANIMATION and describe what happens below:
Some Factors That Affect Enzyme Action
18. The conformation of an enzyme is maintained by interactions between the various
___________________ _______________ that compose it, and this conformation is
sensitive to ________________________________________. Two important influences
are _________________ and ________________. When an enzyme's
________________________ is significantly altered because of ____________ or
___________________ variation, the enzyme may no longer _____________________
___________________. An enzyme is said to be _____________________ when it loses
its functional shape.
pH and Enzyme Function
19. Each enzyme functions best within a _______________________. For example, the
enzyme__________________, which works in your stomach, functions best in a strongly
____________________environment. Lipase, an enzyme found in your
_____________________________, works best in a __________________
environment.
20. When the pH changes, the active site ____________________________ and affects
____________________ ________________. What happens to catalysis when an enzyme
is subjected to a pH far from its optimum range?
21. WATCH THE ANIMATION AND DESCRIBE WHAT YOU SEE BELOW:
Temperature and Enzyme Function
22. Chemical reactions speed up as ____________________ is increased, so, in
general,_________________ will ___________________ at higher temperatures.
However, each enzyme has a temperature __________________________, and beyond
this point the enzyme's _____________________________ is lost.
___________________ temperatures will ______________________ most enzymes.
WEBSITE #3
http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/E/Enzymes.html#pHandTemp
23. Identify what you learned about Enzymes and how they are affected BY TEMPERATURE
and pH by observing the graphs on this website.
WEBSITE #4
http://ull.chemistry.uakron.edu/genobc/animations/enzyme.mov (non funny version)
http://ull.chemistry.uakron.edu/genobc/animations/enzyme_fun.mov (funny version)
24. What does the BLUE molecule represent?____________
25. What does the YELLOW molecule represent?____________
26. What does the RED DOT on the BLUE molecule represent?___________
27. What do the GREEN and PURPLE molecules represent?________________
28. What did you observe? An enzyme SYNTHESIS or LYSIS reaction?
WEBSITE #5:http://bcs.whfreeman.com/thelifewire/content/chp06/0602001.html
GO TO ANIMATION, then to STEP THROUGH
1st-Add a SUBSTRATE
2nd-Add an ENZYME
PRESS PLAY
29. Describe what happened:
1st-Add a SUBSTRATE
2nd-Add an ENZYME
3rd-Add a COMPETITIVE INHIBITOR
PRESS PLAY
30. Describe what happened:
1st-Add a SUBSTRATE
2nd-Add an ENZYME
3rd-Add a NON-COMPETITIVE INHIBITOR
PRESS PLAY
31. Describe what happened:
32. What is the difference between a NONCOMPETITIVE IHIBITOR AND A COMPETITIVE
INHIBITOR?
WEBSITE #6: http://bcs.whfreeman.com/thelifewire/content/chp06/0602002.html
Make sure you are in the ANIMATION portion and go to STEP THROUGH…Watch the
animation…it is a bit complicated. Main idea I need you to understand is….
33. TRUE or FALSE: an enzyme can change shape and become ACTIVE or INACTIVE.
WEBSITE #7: http://highered.mcgrawhill.com/sites/0072437316/student_view0/chapter8/animations.html#
34. Click on BIOCHEMICAL PATHWAY, MAKE SURE YOUR VOLUME IS TURNED UP…what A
BIOCHEMICAL PATHWAY?
Now click on Feeback Inhibition
35. What is Feedback Inhibition?
36. What is an ALLOSTERIC SITE?
37. Why might this process take place?
WEBSITE #8: THIS is DIFFICULT…but you need to get the GENERAL IDEA! MAKE SURE
YOUR VOLUME IT UP….SO YOU CAN HEAR THE SITE!
http://www.wiley.com/college/pratt/0471393878/student/animations/enzyme_inhibition/inde
x.html
38. HOW ENZYME INHIBITORS ACT AS DRUGS!
CASE I: HOW ASPIRIN AND OTHER NONSTEROIDAL ANTI-INFLAMMATORY
INHIBITORS WORKS
Nonsteroidal anti-inflammatory drugs work by interfering with the cyclooxygenase pathway.
The normal process begins with arachidonic acid, a dietary unsaturated fatty acid obtained
from animal fats. This acid is converted by the enzyme cyclooxygenase to synthesize
different prostaglandins. The prostaglandins go on to stimulate many other regulatory
functions and reactionary responses in the body including: inflammation and increased
sensitivity to pain . Aspirin and other NSAID's work by inhibiting this pathway.
39. EXPLAIN IN YOUR OWN WORDS….HOW ASPRIN BLOCKS INFLAMMATION!
WEBSITE #9: http://en.wikipedia.org/wiki/Enzyme_inhibitor
40. Explain what an enzyme inhibitor is.
41. Enzyme inhibitors occur naturally in your cells. Explain how enzyme inhibitors maintain
homeostasis in your body.
WEBSITE #10: http://depression.about.com/od/depressionmedication1/f/howadswork.htm
42. True or False: Anti-depressants are enzyme inhibitors.