Teacher Guide: Fast Plants® 1 – Growth and Genetics Learning Objectives Students will … Observe the life cycle of Wisconsin Fast Plants®, from seed to mature seedpod. Use a “bee stick” to pollinate Fast Plants. Design experiments to test the effects of water, light, and crowding on Fast Plants. Observe Fast Plants traits for stem color and leaf color. Identify dominant and recessive alleles for stem and leaf color. Use Punnett squares to predict the percentages of different offspring genotypes and phenotypes, then test these predictions using the Gizmo. Vocabulary allele, dominant allele, Fast Plants, gene, genetics, genotype, heterozygous, homozygous, offspring, phenotype, pollen, pollinate, Punnett square, recessive allele, trait Lesson Overview The Fast Plants® Gizmos were created as a collaboration between ExploreLearning and the Wisconsin Fast Plants Program of the University of Wisconsin-Madison. They were designed to support the experiments that students can do using Fast Plants seeds and plants. By using these Gizmos in combination with growing Fast Plants, students can compare simulated growth, development and reproduction with firsthand observations of living Fast Plants. The Student Exploration sheet for Fast Plants 1 – Growth and Genetics contains three activities: Activity A – Students learn about the life cycles of Fast Plants (flowering plants) and experiment with different growing conditions. Activity B – Students make observations of how stem and leaf color traits in Fast Plants are passed from parent to offspring plants. Activity C – Students learn about the underlying genetics that regulate stem and leaf color, then use Punnett squares to predict the ratios of traits in offspring. Suggested Lesson Sequence 1. Pre-Gizmo activities ( varies) The best way to prepare for the Fast Plants 1 Gizmo is to grow Wisconsin Fast Plants® in the classroom. Fast Plants grow from seed to flowering in just two weeks, under standard growing conditions. Complete information about growing Fast Plants is available on the Program’s website: www.fastplants.org, and seeds are available from Carolina Biological Supply®. To replicate some of the experiments in the Gizmo, students can grow Purple and Non-Purple Stem, and Yellow-Green Leaf varieties of Wisconsin Fast Plants Plants. Students can then use “bee sticks” or other tools to pollinate their living plants, produce seedpods, and harvest the mature seeds. 2019 It is helpful for students to understand the basics of Mendelian genetics and the use of Punnett squares prior to using this Gizmo. The Mouse Genetics (One Trait) and Mouse Genetics (Two Traits) Gizmos would be great preparation. 2. Prior to using the Gizmo ( 10 – 15 minutes) Before students are at the computers, pass out the Student Exploration sheets and ask them to complete the Prior Knowledge Questions. Discuss student answers as a class, but do not provide correct answers at this point. Afterwards, if possible, use a projector to introduce the Gizmo and demonstrate its basic operations. 3. Gizmo activities ( 15 – 20 minutes per activity) Assign students to computers. Students can work individually or in small groups. Ask students to work through the activities in the Student Exploration using the Gizmo. Alternatively, you can use a projector and do the Exploration as a teacher-led activity. 4. Discussion questions ( 15 – 30 minutes) As students are working or just after they are done, discuss the following questions: What does a plant need to grow and reproduce, and how is this illustrated in the Gizmo? [Plants need soil, light, and water to grow. In the Gizmo, the plants do not grow if there is no water or light. For the plant to reproduce, the flowers must be pollinated. In the Gizmo, plants that are not pollinated do not produce seeds.] Why is a “bee stick” used for flower pollination? [Bees collect pollen on the hairs that cover their bodies (setae) and in pollen baskets on their legs. A bee stick consists of a dried bee or bee thorax (the "hairiest" part of a bee) on a toothpick.] Suppose a heterozygous green leaf plant is pollinated with pollen from a yellowgreen leaf plant. What proportion of the offspring will have yellow-green leaves? [About half. All offspring receive a ygr allele from the yellow-green parent. Half the offspring receive YGR alleles from the green parent, forming YGR/ygr (green) offspring. The rest receive ygr alleles, forming ygr/ygr (yellow-green) offspring.] Suppose your classmate proposes that each plant had only one allele for each trait. For example, a purple-stem plant would have just one ANL allele and a nonpuple (green) stem plant would have one anl allele. When the plants mate, offspring plants only have the dominant ANL allele. What observation disproves this theory? [In the Gizmo, the F1 generation all had purple stems, but some the F2 plants had green stems. This shows that the recessive anl allele did not disappear and must have been carried by the F1 plants.] 5. Follow-up activities ( varies) If possible, obtain Fast Plants® seeds and grow the plants to replicate some of the experiments shown in this Gizmo. In addition to the homozygous parent seeds (purple stem/green leaf and non-purple stem/yellow-green leaf) there are F1 (ANL/anl, YGR/ygr) and F2 (offspring of F1 plants) seed packs available. The Fast Plants 2 – Mystery Parent activity is a follow-up to the Fast Plants 1 activities. In this lesson, students will determine the genotype and phenotype of a mystery parent based on the traits of offspring plants and the other parent plants. Students can then complete challenges to design their own Fast Plant hybrids. Fast Plants 2 also offers an opportunity for students to learn about and apply the chi-squared test. 2019 Scientific Background: Brassica rapa and its subspecies have been selectively bred by humans for over 4,000 years. Cultivars of Brassica rapa include the turnip, bok choy, and napa cabbage. Wisconsin Fast Plants® are a rapid-cycling variety of Brassica rapa developed by Dr. Paul Williams at the University of Wisconsin-Madison. By selectively breeding plants with shorter life cycles, after 20 years he was able to reduce the life cycle from 6 months to 5 weeks. Additional selective breeding allowed various genetic traits in the plants to be isolated and studied. Today, these plants are used for genetic research and as a teaching tool in classrooms all over the world. In the Fast Plants 1 Gizmo, traits for stem color and leaf color are studied. The stem color is determined by the presence or absence of anthocyanin, a purple pigment found in many plants including blueberries, cherries, and red cabbage. The gene, “anthocyaninless,” is named after the recessive trait of producing no anthocyanin. The two alleles are ANL, which produces purple-stemmed plants, and anl, which produces non-purple, or green-stemmed plants. For leaf color, the gene also is named after the recessive trait: yellow-green leaves. Plants with the dominant YGR allele have the standard, dark green leaves, while those with two copies of the recessive ygr allele have yellow-green leaves. (Note: Students are usually taught that allele symbols are based on the dominant allele. In fact, most allele symbols are based on the less common, or “mutant” allele. If this allele is recessive, the symbol is based on the recessive trait.) To predict the offspring of a dihybrid (two traits) cross, use an expanded Punnett square. The sides of the square show the possible allele combinations contributed by each parent. The boxes in the square show the proportion of offspring that will inherit that allele combination. For example, the Punnett square at right shows the possible offspring of an ANL/anl, YGR/ygr plant and an ANL/anl, ygr/ygr plant. Based on the Punnett square, you could predict that about 6 of 16 offspring (37.5%) will have purple stems and yellow leaves. You could also predict that about 2 of 16 offspring (12.5%) will have green stems and green leaves. Selected Web Resources Wisconsin Fast Plants: https://fastplants.org/ Fast Plants inheritance: https://fastplants.org/category/fast-plants-inheritance-blog/ Carolina Biological Supply (source for Fast Plants seeds): https://www.carolina.com/ Mendelian genetics: https://www2.palomar.edu/anthro/mendel/mendel_1.htm Dihybrid cross: https://www.ndsu.edu/pubweb/~mcclean/plsc431/mendel/mendel3.htm, http://www.biology.arizona.edu/mendelian_genetics/problem_sets/dihybrid_cross/03t.html Growing Plants Gizmo: https://www.explorelearning.com/gizmo/id?635 Pollination: Flower to Fruit Gizmo: https://www.explorelearning.com/gizmo/id?615 Mouse Genetics (One Trait) Gizmo: https://www.explorelearning.com/gizmo/id?449 Mouse Genetics (Two Traits) Gizmo: https://www.explorelearning.com/gizmo/id?382 Fast Plants 2 – Mystery Parent Gizmo: https://www.explorelearning.com/gizmo/id?1087 2019