1 Instructor’s Manual to accompany Hole’s Human Anatomy and Physiology Laboratory Manual, Cat Version and Fetal Pig Version Fifteenth Edition Terry R. Martin Kishwaukee College Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 2 CONTENTS PREFACE...................................................................................................................................................................................................... 5 AN OVERVIEW............................................................................................................................................................................................. 6 INSTRUCTIONAL APPROACHES …............................................................................................................................................................ 7 CORRELATION OF TEXTBOOK SYSTEMS AND SUPPLEMENTAL LABORATORY EXERCISES 8 ......................................................... SUGGESTED TIME SCHEDULE ................................................................................................................................................................ 11 Fundamentals of Human Anatomy and Physiology Laboratory Exercise 1 – Scientific Method and Measurement ..................................................................................................................... 12 Laboratory Exercise 2 – Body Organization and Terminology ..................................................................................................................... 13 Laboratory Exercise 3 – Chemistry of Life ................................................................................................................................................... 15 Laboratory Exercise 4 – Care and Use of the Microscope 16 .......................................................................................................................... Cells Laboratory Exercise 5 – Cell Structure and Function ................................................................................................................................... 17 Laboratory Exercise 6 – Movements Through Membranes ......................................................................................................................... 18 Laboratory Exercise 7 – Cell Cycle .............................................................................................................................................................. 19 Tissues Laboratory Exercise 8 – Epithelial Tissues .................................................................................................................................................. 20 Laboratory Exercise 9 – Connective Tissues 21 ............................................................................................................................................. Laboratory Exercise 10 – Muscle and Nervous Tissues 22 ............................................................................................................................ Integumentary System Laboratory Exercise 11 – Integumentary System 23 ...................................................................................................................................... Skeletal System Laboratory Exercise 12 – Bone Structure and Classification 24 Laboratory Exercise 13 – Organization of the Skeleton 25 Laboratory Exercise 14 – Skull 26 ..................................................................................................................... ............................................................................................................................. .................................................................................................................................................................. Laboratory Exercise 15 – Vertebral Column and Thoracic Cage 28 Laboratory Exercise 16 – Pectoral Girdle and Upper Limb 30 Laboratory Exercise 17 – Pelvic Girdle and Lower Limb 32 Laboratory Exercise 18 – Joint Structure and Movements 34 .............................................................................................................. ........................................................................................................................ ........................................................................................................................... ......................................................................................................................... Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 3 Muscular System Laboratory Exercise 19 – Skeletal Muscle Structure and Function............................................................................................................... 35 Laboratory Exercise 20 – Muscles of the Head and Neck……….................................................................................................................. 36 Laboratory Exercise 21 – Muscles of the Chest, Shoulder, and Upper Limb 37 ............................................................................................. Laboratory Exercise 22 – Muscles of the Deep Back, Abdominal Wall, and Pelvic Floor ........................................................................... 38 Laboratory Exercise 23 – Muscles of the Hip and Lower Limb 39 ................................................................................................................... Surface Anatomy Laboratory Exercise 24 – Surface Anatomy 40 ............................................................................................................................................... Nervous System Laboratory Exercise 25 – Nervous Tissue and Nerves 42 Laboratory Exercise 26 – Brain and Cranial Nerves 43 ............................................................................................................................. .................................................................................................................................. Laboratory Exercise 27 – Dissection of the Sheep Brain 44 Laboratory Exercise 28 – Spinal Cord and Meninges 45 Laboratory Exercise 29 – Reflex Arc and Reflexes 46 ........................................................................................................................... .............................................................................................................................. ................................................................................................................................... General and Special Senses Laboratory Exercise 30 – Receptors and General Senses 47 ....................................................................................................................... Laboratory Exercise 31 – Smell and Taste 48 ................................................................................................................................................. Laboratory Exercise 32 – Ear and Hearing 49 ................................................................................................................................................. Laboratory Exercise 33 – Ear and Equilibrium 50 Laboratory Exercise 34 – Eye Structure 51 ............................................................................................................................................ .................................................................................................................................................... Laboratory Exercise 35 – Visual Tests and Demonstrations 53 ...................................................................................................................... Endocrine System Laboratory Exercise 36 – Endocrine Histology and Diabetic Physiology 54 ................................................................................................... Cardiovascular System Laboratory Exercise 37 – Blood Cells and Blood Typing 56 ........................................................................................................................... Laboratory Exercise 38 – Heart Structure 57 ................................................................................................................................................... Laboratory Exercise 39 – Cardiac Cycle 59 .................................................................................................................................................... Laboratory Exercise 40 – Blood Vessel Structure, Arteries, and Veins 60 Laboratory Exercise 41 – Pulse Rate and Blood Pressure 62 ..................................................................................................... ........................................................................................................................ Lymphatic System Laboratory Exercise 42 – Lymphatic System 63 ............................................................................................................................................. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 4 Digestive System Laboratory Exercise 43 – Digestive Organs 64 .............................................................................................................................................. Laboratory Exercise 44 – Action of a Digestive Enzyme 66 ............................................................................................................................ Respiratory System Laboratory Exercise 45 – Respiratory Organs 67 ........................................................................................................................................... Laboratory Exercise 46 – Breathing and Respiratory Volumes 68 Laboratory Exercise 47 – Control of Breathing 69 .................................................................................................................. ........................................................................................................................................... Urinary System Laboratory Exercise 48 – Urinary Organs……. 70 Laboratory Exercise 49 – Urinalysis 71 ........................................................................................................................................... .......................................................................................................................................................... Reproductive Systems and Development Laboratory Exercise 50 – Male Reproductive System 72 ............................................................................................................................... Laboratory Exercise 51 – Female Reproductive System 73 ............................................................................................................................ Laboratory Exercise 52 – Fertilization and Early Development 74 Laboratory Exercise 53 – Genetics 75 .................................................................................................................. ............................................................................................................................................................ Cat or Fetal Pig Dissection Laboratory Exercise 54 – Cat (or Fetal Pig) Dissection: Musculature 77 ........................................................................................................ Laboratory Exercise 55 – Cat (or Fetal Pig) Dissection: Cardiovascular System 79 Laboratory Exercise 56 – Cat (or Fetal Pig) Dissection: Digestive System 81 ................................................................................................ Laboratory Exercise 57 – Cat (or Fetal Pig) Dissection: Respiratory System 83 Laboratory Exercise 58 – Cat (or Fetal Pig) Dissection: Urinary System 85 ...................................................................................... ............................................................................................ ................................................................................................... Laboratory Exercise 59 – Cat (or Fetal Pig) Dissection: Reproductive Systems 87 ....................................................................................... Supplemental Laboratory Exercises (these laboratory exercises are available online) Laboratory Exercise 60 – Skeletal Muscle Contraction 89 Laboratory Exercise 61 – Nerve Impulse Stimulation 90 Laboratory Exercise 62 – Blood Testing 91 .............................................................................................................................. ................................................................................................................................. .................................................................................................................................................... Laboratory Exercise 63 – Factors Affecting the Cardiac Cycle 92 Appendix 1: Materials Needed 93 ................................................................................................................... .................................................................................................................................................................... Appendix 2: Laboratory Suppliers 96 .............................................................................................................................................................. Appendix 3: Student Safety Rules Agreement 98 ............................................................................................................................................ Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 5 Appendix 4: Student Informed Consent Form 99 ............................................................................................................................................. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 6 PREFACE This instructor’s manual is designed to assist those who are using the Laboratory Manual to Accompany Hole’s Human Anatomy and Physiology, cat version and fetal pig version, fifteenth edition by Terry R. Martin. It describes the purpose of the laboratory manual and its special features, and provides suggestions for presenting the laboratory exercises to students. The instructor’s manual also parallels the laboratory manual, exercise by exercise, providing labels for unlabeled diagrams and answers to questions that appear in the laboratory reports. For some exercises, special instructional suggestions that propose alternative procedures, laboratory equipment, or laboratory techniques are provided. Most of the illustrations and labels parallel the textbook very closely as requested by many of the users of the laboratory manual. Many of the leader lines are arranged differently than the book, and several illustrations are different than the textbook. This has been requested also by many of the users of the laboratory manual. I have attempted to reach a balance that will be beneficial for all students and instructors. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 7 AN OVERVIEW The Laboratory Manual to Accompany Hole’s Human Anatomy and Physiology, cat version and fetal pig version, fifteenth edition, was written to accompany the textbook Hole’s Human Anatomy and Physiology, fifteenth edition, by Shier, Butler, and Lewis. As in the case of the textbook, the laboratory manual is planned for students pursuing careers in allied health fields who have minimal backgrounds in the physical and biological sciences. The manual contains fifty-nine laboratory exercises and four online supplemental laboratory exercises that are closely integrated with the content of a textbook (four of these exercises only appear online via the text McGraw-Hill Connect site). The exercises are designed to review and illustrate various anatomical and physiological facts and principles presented in the textbook and to help students investigate some of these ideas in more detail. The laboratory exercises include a variety of special features that are designed to stimulate student interest in the subject matter, to involve students in the learning process, and to guide them through the planned experiences. These features include the following: Materials Needed. The laboratory materials listed are those that students require to complete the exercise and to perform the learning activities. Safety. If the laboratory exercise requires special safety guidelines, this section is included. Laboratory Safety Guidelines also appear as Appendix 1 of your laboratory manual. Some institutions might have committees for reviewing labs using live animals, biohazards, and physiology experiments performed on students. Be sure your labs conform to their guidelines. You might find the forms available in Appendixes 3 and 4 useful at your school. Purpose of the Exercise. The purpose provides a statement concerning the intent of the exercise—that is, what will be accomplished. Learning Outcomes. The learning outcomes list in general terms what a student should be able to do after completing the exercise. Introduction. The introduction briefly describes the subject of the exercise or the ideas that will be investigated. Procedure. The procedure provides a set of detailed instructions for accomplishing the planned laboratory activities. Usually these instructions are presented in outline form so that a student can proceed through the exercise in stepwise fashion. Frequently, the student is referred to particular sections of a textbook for necessary background information or for review of subject matter presented in some previous part of the course. The procedures include a wide variety of laboratory activities and, from time to time, direct the student to complete various tasks in the laboratory reports. Learning Activities. Learning activities appear in separate boxes as Learn: Activity or Learn: Lab in Motion. They are planned to encourage students to extend their laboratory experiences. Some of these activities are open-ended in that they suggest how a student can plan an investigation or experiment and carry it out after receiving approval from the laboratory instructor. Illustrations. Diagrams are used as aids for reviewing subject matter. Other illustrations provide visual instructions for performing steps in procedures or are used to identify parts of instruments or specimens. Micrographs often are included to help students identify microscopic structures or to evaluate student understanding of tissues. Some figures, such as the skull, are presented so that they are suitable for coloring. You may want to have your students use colored pencils to highlight various parts of these illustrations. This activity should enhance their ability to observe the figures more carefully and help them locate and identify important anatomical features. Laboratory Reports. Immediately following each exercise, there is a laboratory report to be completed by the student. These reports include various types of review activities, spaces for sketches of microscopic objects, tables for recording observations and experimental results, and questions dealing with the analysis of such data. As a result of these laboratory exercises, students should develop a better understanding of the structural and functional characteristics of their bodies. In addition, their skills in gathering information by observation and experimentation should increase. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 8 INSTRUCTIONAL APPROACHES Exercise Selection Although the laboratory manual contains fifty-nine separate exercises, it may not be possible to include all of them in any one program. However, because many of the exercises are relatively short and because the procedures of others are divided into sections, an instructor can easily select those exercises or parts of exercises that best meet the needs of a particular class. These exercises also vary in the quantities of equipment needed to complete them; if necessary, an instructor can make some selection based upon the amount of laboratory equipment available for use by a class. Animal Dissection In the laboratory manual, the preserved cat or fetal pig is used as the major animal to be dissected. Detailed instructions for dissecting certain organs, such as the sheep brain, sheep heart, pig kidney, and mammalian eye are also included. A laboratory option is to obtain a cadaver as a demonstration specimen. If this is not possible, consider a field trip to a location that has a prosected cadaver. A minimum of two viewings is recommended—one during muscle study and the other near the end of the course. The Use of Animals in Biology Education* The National Association of Biology Teachers (NABT) believes that the study of organisms, including nonhuman animals, is essential to the understanding of life on Earth. NABT recommends the prudent and responsible use of animals in the life science classroom. NABT believes that biology teachers should foster a respect for life. Biology teachers also should teach about the interrelationship and interdependency of all things. Classroom experiences that involve nonhuman animals range from observation to dissection. NABT supports these experiences so long as they are conducted within the long-established guidelines of proper care and use of animals, as developed by the scientific and educational community. As with any instructional activity, the use of nonhuman animals in the biology classroom must have sound educational objectives. Any use of animals, whether for observation or dissection, must convey substantive knowledge of biology. NABT believes that biology teachers are in the best position to make this determination for their students. NABT acknowledges that no alternative can substitute for the actual experience of dissection or other use of animals and urges teachers to be aware of the limitations of alternatives. When the teacher determines that the most effective means to meet the objectives of the class do not require dissection, NABT accepts the use of alternatives to dissection including models and the various forms of multimedia. The Association encourages teachers to be sensitive to substantive student objections to dissection and to consider providing appropriate lessons for those students where necessary. To implement this policy, NABT endorses and adopts the “Principle and Guidelines for the use of Animals in Precollege Education” of the Institute of Laboratory Animals Resources (National Research Council). Copies of the “Principle and Guidelines” may be obtained from the ILAR (2101 Constitution Avenue, NW, Washington DC 20418; 202-334-2590). *Adopted by the Board of Directors in October 1995. This policy supersedes and replaces all previous NABT statements regarding animals in biology education. Background Information The procedures of many exercises begin by suggesting that students review specific sections of the textbook. If the subject matter involved in a particular exercise has been covered recently in lecture, the students may be able to accomplish such a review rather quickly. On the other hand, if the material has not been presented previously, this part of a procedure may be used as a means of introducing information needed to understand the ideas presented in the exercise. When the procedure is used to introduce new material, an instructor may ask students to complete the first section before coming to the laboratory. Following this, some portion of the laboratory time may be needed for class discussion of the new material. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 9 CORRELATION OF TEXTBOOK CHAPTERS AND LABORATORY EXERCISES Textbook Chapters Ch. 1 Introduction to Human Anatomy and Physiology Related Laboratory Exercises Ex. 1 Scientific Method and Measurements Ex. 2 Body Organization and Terminology Ch. 2 Chemical Basis of Life Ex. 3 Chemistry of Life Ch. 3 Cells Ex. 4 Ex. 5 Ex. 6 Ex. 7 Care and Use of the Microscope Cell Structure and Function Movements Through Membranes Cell Cycle Ch. 4 Cellular Metabolism Ch. 5 Tissues Ex. 8 Ex. 9 Ex. 10 Epithelial Tissues Connective Tissues Muscle and Nervous Tissues Ch. 6 Integumentary System Ex. 11 Integumentary System Ch. 7 Skeletal System Ex. 12 Ex. 13 Ex. 14 Ex. 15 Ex. 16 Ex. 17 Bones and Structure and Classification Organization of the Skeleton Skull Vertebral Column and Thoracic Cage Pectoral Girdle and Upper Limb Pelvic Girdle and Lower Limb Ch. 8 Joints of the Skeletal System Ex. 18 Joint Structure and Movements Ch. 9 Muscular System Ex. 19 Ex. 20 Ex. 21 Ex. 22 Ex. 23 Ex. 24 Ex. 54 Skeletal Muscle Structure and Function Muscles of the Head and Neck Muscles of the Chest, Shoulder, and Upper Limb Muscles of the Deep Back, Abdominal Wall, and Pelvic Floor Muscles of the Hip and Lower Limb Surface Anatomy Cat (or Fetal Pig) Dissection: Musculature Ch. 10 Nervous System I: Basic Structure and Function Ex. 25 Nervous Tissue and Nerves Ch. 11 Nervous System II: Divisions of the Nervous System Ex. 26 Ex. 27 Ex. 28 Ex. 29 Brain and Cranial Nerves Dissection of the Sheep Brain Spinal Cord and Meninges Reflex Arc and Reflexes Ch. 12 Nervous System III: Senses Ex. 30 Ex. 31 Ex. 32 Ex. 33 Ex. 34 Ex. 35 Receptors and General Senses Smell and Taste Ear and Hearing Ear and Equilibrium Eye Structure Visual Tests and Demonstrations Ch. 13 Endocrine System Ex. 36 Endocrine Histology and Diabetic Physiology Ch. 14 Blood Ex. 37 Blood Cells and Blood Typing Ch. 15 Cardiovascular System Ex. 38 Ex. 39 Ex. 40 Ex. 41 Ex. 55 System Heart Structure Cardiac Cycle Blood Vessel Structure, Arteries, and Veins Pulse Rate and Blood Pressure Cat (or Fetal Pig) Dissection: Cardiovascular Ch. 16 Lymphatic System and Immunity Ex. 42 Lymphatic System Ch. 17 Digestive System Ex. 43 Digestive Organs Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 10 Ex. 44 Ex. 56 Action of a Digestive Enzyme Cat (or Fetal Pig) Dissection: Digestive System Ch. 18 Nutrition and Metabolism Ch. 19 Respiratory System Ex. 45 Ex. 46 Ex. 47 Ex. 56 Respiratory Organs Breathing and Respiratory Volumes Control of Breathing Cat (or Fetal Pig) Dissection: Digestive System Ch. 20 Urinary System Ex. 48 Ex. 49 Ex. 58 Urinary Organs Urinalysis Cat (or Fetal Pig) Dissection: Urinary System Ch. 21 Water, Electrolyte, and Acid-Base Balance Ch. 22 Reproductive Systems Ex. 50 Ex. 51 Ex. 59 Male Reproductive System Female Reproductive System Cat (or Fetal Pig) Dissection: Reproductive Systems Ch. 23 Pregnancy, Growth, and Development Ex. 52 Fertilization and Early Development Ch. 24 Genetics and Genomics Ex. 53 Genetics Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 11 CORRELATION OF TEXTBOOK CHAPTERS AND SUPPLEMENTAL LABORATORY EXERCISES Ch. 9 Muscular System Ex. 60 Skeletal Muscle Contraction Ch. 10 Nervous System I: Basic Structure and Function Ex. 61 Nerve Impulse Stimulation Ch. 14 Blood Ex. 62 Blood Testing Ch. 15 Cardiovascular System Ex. 63 Factors Affecting the Cardiac Cycle Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 12 SUGGESTED TIME SCHEDULE Different instructional programs provide different lengths of time for laboratory preparations, work activities, and follow-up discussions. Other factors that influence the time required for each exercise are the availability and variety of laboratory equipment and materials. Consequently, it is difficult to make precise suggestions for the amounts of time that should be set aside for particular laboratory exercises. The suggested time schedule was prepared with these limitations in mind. The hours listed for each exercise indicate the minimal time that probably will be needed for students who are acquainted with the subject matter of the exercise to complete the laboratory work. Students who lack background information and who have to read various sections of the textbook before beginning an exercises probably will require additional time. Similarly, students who are expected to complete the laboratory reports in class may need more time. Laboratory Exercise Minimal Time Ex. 1 Scientific Method and Measurements Ex. 2 Body Organization and Terminology Ex. 3 Chemistry of Life Ex. 4 Care and Use of the Microscope Ex. 5 Cell Structure and Function Ex. 6 Movements Through Membranes Ex. 7 Cell Cycle Ex. 8 Epithelial Tissues Ex. 9 Connective Tissues Ex. 10 Muscle and Nervous Tissues Ex. 11 Integumentary System Ex. 12 Bone Structure and Classification Ex. 13 Organization of the Skeleton Ex. 14 Skull Ex. 15 Vertebral Column and Thoracic Cage 2 hr. Ex. 16 Pectoral Girdle and Upper Limb Ex. 17 Pelvic Girdle and Lower Limb Ex. 18 Joint Structure and Movements Ex. 19 Skeletal Muscle Structure and Function Ex. 20 Muscles of the Head and Neck Ex. 21 Muscles of the Chest, Shoulder, and Upper Limb Ex. 22 Muscles of the Deep Back, Abdominal Wall, and Pelvic Floor Ex. 23 Muscles of the Hip and Lower Limb Ex. 24 Surface Anatomy Ex. 25 Nervous Tissue and Nerves Ex. 26 Brain and Cranial Nerves Ex. 27 Dissection of the Sheep Brain Ex. 28 Spinal Cord and Meninges Ex. 29 Reflex Arc and Reflexes Ex. 30 Receptors and General Senses Ex. 31 Smell and Taste Ex. 32 Ear and Hearing 2 hr. 2 hr. 2 hr. 2 hr. 2 hr. 3 hr. 1 hr. 2 hr. 2 hr. 1 hr. 1 hr. 1 hr. 1 hr. 3 hr. 2 hr. 2 hr. 2 hr. 1 hr. 1 hr. 2 hr. 1 hr. 2 hr. 2 hr. 2 hr. 2 hr. 2 hr. 1 hr. 1 hr. 2 hr. 2 hr. 2 hr. Minimal Time Laboratory Exercise Ex. 33 Ex. 34 Ex. 35 Ex. 36 Ex. 37 Ex. 38 Ex. 39 Ex. 40 Ex. 41 Ex. 42 Ex. 43 Ex. 44 Ex. 45 Ex. 46 Ex. 47 Ex. 48 Ex. 49 Ex. 50 Ex. 51 Ex. 52 Ex. 53 Ex. 54 Ex. 55 Ear and Equilibrium 1 hr. Eye Structure 3 hr. Visual Tests and Demonstrations 2 hr. Endocrine Histology and Diabetic Physiology 3 hr. Blood Cells and Blood Typing 3 hr. Heart Structure 2 hr. Cardiac Cycle 3 hr. Blood Vessel Structure, Arteries, and Veins 3 hr. Pulse Rate and Blood Pressure 2 hr. Lymphatic System 2 hr. Digestive Organs 2 hr. Action of a Digestive Enzyme 2 hr. Respiratory Organs 2 hr. Breathing and Respiratory Volumes 1 hr. Control of Breathing 1 hr. Urinary Organs 2 hr. Urinalysis 3 hr. Male Reproductive System 2 hr. Female Reproductive System 2 hr. Fertilization and Early Development 2 hr Genetics 2 hr. Cat (or Fetal Pig) Dissection: Musculature 6 hr. Cat (or Fetal Pig) Dissection: Cardiovascular System 3 hr. Cat (or Fetal Pig) Dissection: Digestive System 2 hr. Cat (or Fetal Pig) Dissection: Respiratory System 2 hr. Cat (or Fetal Pig) Dissection: Urinary System 1 hr. Cat (or Fetal Pig) Dissection: Reproductive Systems 2 hr. Skeletal Muscle Contraction 3 hr. Nerve Impulse Stimulation 3 hr. Blood Testing 2 hr. Factors Affecting the Cardiac Cycle 3 hr. Ex. 56 Ex. 57 Ex. 58 Ex. 59 Ex. 60 Ex. 61 Ex. 62 Ex. 63 Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 13 LABORATORY EXERCISE 1 SCIENTIFIC METHOD AND MEASUREMENTS Assess: Critical Thinking Answers Answers and data will vary. Laboratory Report Answers PART A 1. (experimental results) 2. (experimental results) 3. Answers will vary, however many students will conclude that the data will support the original hypothesis. PART B 1 – 6. Answers will vary Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 14 LABORATORY EXERCISE 2 BODY ORGANIZATION AND TERMINOLOGY Instructional Suggestions If a dissectible human torso model (manikin) is not available, you might want to have students consult the figures in various sections of a textbook, particularly the body sections in the reference plates, to gain some understanding of the organizational pattern of the human body. Figure Labels FIG. 2.1 1. Thoracic cavity 2. Abdominal cavity 3. Abdominopelvic cavity 4. 5. 6. Pelvic cavity Cranial cavity Vertebral canal (spinal cavity) FIG. 2.2.a 1. Visceral pleura 2. Pleural cavity 3. Parietal pleura 4. 5. 6. Visceral pericardium (epicardium) Pericardial cavity Parietal pericardium FIG. 2.2b 7. Visceral peritoneum 8. Peritoneal cavity 9. Parietal peritoneum FIG. 2.5 1. Sagittal plane (median; midsagittal plane) 2. Frontal (coronal) plane 3. Transverse (horizontal) plane FIG. 2.6a 1. Epigastric region 2. Right hypochondriac region 3. Right lateral (lumbar) region 4. Umbilical region 5. Right inguinal (iliac) region 6. 7. 8. 9. Left hypochondriac region Left lateral (lumbar) region Left inguinal (iliac) region Pubic (hypogastric) region 12. 13. Left upper quadrant (LUQ) Left lower quadrant (LLQ) FIG. 2.6b 10. Right upper quadrant (RUQ) 11. Right lower quadrant (RLQ) FIG. 2.7a 1. Nasal 2. Oral 3. Cervical 4. Acromial 5. Axillary 6. Mammary 7. Brachial 8. 9. 10. 11. 12. 13. 14. Antecubital Abdominal Antebrachial Carpal Palmar Digital Gential 15. 16. 17. 18. 19. 20. 21. Crural Tarsal Cephalic Frontal Orbital Buccal Mental 22. 23. 24. 25. 26. 27. 28. FIG. 2.7b 1. Otic 2. Occipital 3. Acromial 4. Vertebral 5. 6. 7. 8. Brachial Dorsum Cubital Lumbar 9. 10. 11. 12. Sacral Gluteal Perineal Femoral 13. Popliteal 14. Sural (calf) 15. Plantar Sternal Pectoral Umbilical Inguinal Coxal Patellar Pedal Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 15 Laboratory Report Answers PART A 1. a 2. d 3. a 4. 5. 6. a b c 7. 8. 9. a c d 10. e 11. d 12. a PART B 1. c 2. d 3. h 4. 5. 6. g j i 7. 8. 9. e f k 10. b 11. a PART C 1. Inferior 2. (Correct) 3. (Correct) 4. 5. 6. Anterior (Correct) (Correct) 7. 8. 9. Distal (Correct) (Correct) 10. (Correct) 11. Contralateral 12. Posterior PART D 1. e 2. k 3. b 4. 5. 6. i l j 7. 8. 9. g c d 10. h 11. f 12. a PART E 1. l 2. c 3. h 4. 5. 6. i k f 7. 8. 9. j g d 10. a 11. b 12. e 4. 5. 6. RUQ LUQ or LLQ LUQ Assess: Critical Thinking Answers PART F 1. LUQ 2. RLQ 3. Any or all quadrants PART G Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 16 LABORATORY EXERCISE 3 CHEMISTRY OF LIFE Instructional Suggestions 1. The 7 assorted common liquids for the pH tests could include among the following: orange juice, lemon juice, milk, bottled water, baking soda solution, aspirin solution, borax solution, ammonia, vinegar, antacid, liquid soap, cola, and diluted catsup. Any beverage can be used, and any household product, even solids if they can be dissolved. 2. The unknown compounds could include among the following: diluted milk, diluted corn syrup, chicken or beef broth, diluted potato soup, diluted pudding, salad dressing, egg substitute, and diluted yogurt. It is very important to use some numbering code to keep track of which unknown is which. The unknowns often look alike and are easily confused. The students need to be instructed to take care not to contaminate their unknown. They need to be reminded to perform all tests, as students often think if they get one positive result they can stop. Be aware that different brands of food products can contain different ingredients. Inform the students when heating the samples using Benedict’s solution, if there is a protein present it may denature or become solid, which should not be interpreted as a positive result. Laboratory Report Answers PART A (matching) 1. b 2. e 3. h 4. j 5. 6. 7. 8. f i c d PART A (molecules and bonding) 1. 1; 1 3. (sketches) 2. 17; 7 4. No; No PART B 1. (experimental results) 2. (experimental results) 3. No PART C 1. (experimental results) 2. (experimental results) 4. Various substances dissolved in tap water will influence the pH 3. 4. (experimental results) (experimental results) 9. 10. 11. 12. k g a l 5. 6. Ionic (sketches) 5. (experimental results) Assess: Critical Thinking Answers Answers will vary. However, albumin has the highest protein value along with the lowest amount of carbohydrates (starch and sugar.) PART D 1 – 4. (experimental results) Answers will vary depending upon the unknown compound selected. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 17 LABORATORY EXERCISE 4 CARE AND USE OF THE MICROSCOPE Instructional Suggestions 1. To stimulate student interest in use of the microscope, you may want to have students prepare wet mounts of pond water and observe the various forms of life present. A plankton net is a helpful device to concentrate pond organisms. Students can be encouraged to bring samples of pond water to class in preparation for this experiment. 2. You may want to provide students with prepared slides of major human organs to examine as a way of increasing their experience with using the microscope. 3. If oil-immersion objectives are available, you may want to provide students with prepared slides of various forms of bacteria to observe using these objectives. Assess: Critical Thinking Answers Answers will vary depending upon the order of the three colored threads. However, the colored thread on the top will be in focus first, the middle one second, and the bottom one last as the student continues to turn the fine adjustment the same direction. Laboratory Report Answers PART A 1. 100× 2. 1,000× PART B 1. (sketch) 2. About 4.5 mm for scanning power (using 4× objective) 3. About 4,500 micrometers PART C 1. (sketch) 2. About 1.7 mm (using a 10× objective) 3. The diameter of the scanning-power field of view is about 2.6 times greater than that of the low-power field of view. 4. Student is unable to see two adjacent mm lines on the scale in a high-power field of view. PART D 1. f 2. i 3. c 4. a 5. h 6. 7. 8. 9. 10. 4. 5. About 2.2 mm About 2,200 micrometers 5. 6. 7. 8. 9. Light intensity is decreased when high-power objective is used. (sketch) Upside down and reversed from right to left Left Toward the observer j d b g e PART E (sketches) Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 18 LABORATORY EXERCISE 5 CELL STRUCTURE AND FUNCTION Instructional Suggestions 1. Instead of preparing cheek cell slides, you may want to have students prepare slides of plant cells using Elodea leaves or onion skin. 2. If live frogs are available, you may want to pith the frogs and have students prepare wet mounts using small samples of the ciliated epithelium that lines the oral cavity. They also can prepare smears of frog blood and stain the cells with methylene blue, and prepare wet mounts of sperm cells from the testes of the male frogs. You then might provide students with prepared slides of human ciliated epithelium, blood, and sperm cells and have the students compare the frog cells with the human cells. Figure Labels FIG. 5.1 1. Flagellum 2. Centrioles 3. Golgi apparatus 4. Smooth endoplasmic reticulum 5. Nucleus 6. 7. 8. 9. 10. Nuclear envelope Mitochondrion Ribosomes Cell membrane Cilia Assess: Critical Thinking Answers The outer body surface is the same tissue as inside the cheek, however outer surface cells are dead from drying out. Laboratory Report Answers PART A 1. a 2. g 3. k 4. l 5. 6. 7. 8. i f c b 9. 10. 11. 12. PART B 1. (sketch) 3. 2. The wet-mount cells look like shells or “ghosts.” The stained cells made the nucleus and other cellular components more clearly visible. PART C 1. (sketches) 2. They should always notice cytoplasm, nucleus, nuclear envelope, and cell membrane PART D (FIG. 5.4) 1. Nucleolus 2. Chromatin 3. Nuclear envelope 4. Rough endoplasmic reticulum 3. d e h j Yes. The stained cheek cells are essentially the same size and shape; however, the process of cell removal may cause many of the cells to become folded and distorted. Answers will vary. 5. Mitochondria 6. Answers will vary 7. Only nonliving cells can be observed, and only sections of a cell can be observed Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 19 LABORATORY EXERCISE 6 MOVEMENTS THROUGH MEMBRANES Instructional Suggestion Instead of using human blood for Procedure C, you may want to substitute some other type of animal blood obtained from a meat packing house, a veterinarian, or a biological supplier. The hemolysis experiment, using RBCs from a safe source, demonstrates concepts of osmosis, tonicity, and membrane characteristics. Laboratory Report Answers PART A 1. (experimental results) (experimental results) 3. Answers will vary. 1. 2. Assess: Critical Thinking Answers Yes Yes 4. Diffusion is the movement of a substance from an area of higher concentration to an area of lower concentration as a result of molecular motion. 3. No PART B 1. Answers will vary. 4. 2. Answers will vary. 3. Water entered the thistle tube through the membrane, thus increasing the volume of liquid in the tube as a result of osmosis. 1. 2. Assess: Critical Thinking Answers Yes No PART C 1. (sketches) 2. Tube 3. There was a net movement of water out of the cells. PART D 1. Water, glucose, and starch. 2. The tests for glucose and starch were positive. 3. Gravity 4. Charcoal 1. 2. Assess: Critical Thinking Answers No Yes 2. Osmosis is the movement of water molecules from an area of higher concentration to an area of lower concentration through a selectively permeable membrane. 3. Yes 3. 4. Tube 1. There was a net movement of water into the cells. Tube 2. There was no net movement of water into or out of the cells. 5. 6. Pore in the filter paper were too small. Filtration is the movement of substances through a membrane as a result of hydrostatic pressure that is greater on one side of the membrane than on the other side. 3. Yes PART E Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 20 LABORATORY EXERCISE 7 CELL CYCLE Figure Labels FIG. 7.4 1. Chromosome (chromatid) 2. Centromere 3. 4. Centrioles Spindle fiber (microtubules) Assess: Critical Thinking Answers Interphase. Even in rapidly dividing cells interphase is the most prevalent because it requires the longest period of time for growth and duplication of cell structures. Laboratory Report Answers PART A Table: Stage Major Events Occurring Interphase Growth, duplication of cell structures, and normal metabolism take place. Prophase Nuclear envelope and nucleolus disperse; chromatin fibers condense, forming chromosomes (paired chromatids); centrioles move to opposite sides of the cell. Metaphase Chromosomes align midway between centrioles. Anaphase Microtubules pull sister chromatids toward centrioles. Telophase Chromosomes elongate and become chromatin fibers; nuclear envelopes reassemble. Cytokinesis Cell membrane constricts, dividing cell into new cells (daughter cells). PART B (sketches) PART C (FIG. 7.6a-d) 1. a. Metaphase b. Telophase 2. 3 5 2 c. d. Prophase Anaphase 1 4 6 PART D Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 21 Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 22 LABORATORY EXERCISE 8 EPITHELIAL TISSUES Laboratory Report Answers PART A 1. f 2. d 3. c 4. 5. 6. d c d 7. 8. 9. e f b 10. a 11. a 12. e PART B (sketches) Assess: Critical Thinking Answers Stratified squamous epithelium would have excellent protection as it is several cells thick. Pseudostratified columnar epithelium with cilia would provide good movement of mucus and trapped particles away from the lungs. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 23 LABORATORY EXERCISE 9 CONNECTIVE TISSUES Laboratory Report Answers PART A 1. f 2. a 3. c 4. d 5. 6. 7. 8. b e a i 9. 10. 11. 12. h d g j PART B (sketches) Assess: Critical Thinking Answers The spleen is composed of reticular connective tissue. This type of tissue contains only short, thin, collagenous fibers (reticular fibers) that are widely scattered. The result is a delicate distribution of fibers among cells and a fluid ground substance. (Spleen repair is difficult due to the short reticular fibers. A ruptured spleen is sometimes removed in surgery.) Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 24 LABORATORY EXERCISE 10 MUSCLE AND NERVOUS TISSUES Laboratory Report Answers PART A 1. b 2. a 3. d 4. a 5. 6. 7. 8. c b c a 9. 10. 11. 12. d b c d PART B (sketches) Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 25 LABORATORY EXERCISE 11 INTEGUMENTARY SYSTEM Figure Labels FIG. 11.1 1. Epidermis 2. Dermis 3. Subcutaneous layer (hypodermis) 4. 5. 6. Sebaceous gland Arrector pili muscle Hair follicle FIG. 11.2 1. Hair shaft 2. Sebaceous gland 3. Hair root 4. 5. 6. Arrector pili muscle Hair follicle Apocrine sweat gland 7. 8. Merocrine sweat gland Blood vessels Assess: Critical Thinking Answers Melanin granules are concentrated within some of the most superficial living cells of the body. Because melanin absorbs the ultraviolet radiation of sunlight, minimal damaging wavelengths reach the living cells of the dermis. (Most of the melanin granules are orientated on the superficial side of the nucleus that serve as a protective shield of the nucleus of the epidermal cells.) Laboratory Report Answers PART A 1. j 2. l 3. a 4. g 5. 6. 7. 8. c h d i 9. 10. 11. 12. f k e b PART B 1. Answers will vary. 2. Answers will vary. 3. Epidermal cells at the base of the hair follicle divide and grow, pushing older cells outward; as these cells die, they become keratinized parts of the hair. 4. The pigment melanin is produced by melanocytes. PART C 1. The epidermis is the superficial layer composed of stratified squamous epithelium. The dermis is the deep layer of skin composed mostly of irregular dense connective tissue. The subcutaneous layer is beneath the dermis and composed of areolar and adipose connective tissues. 2. Cells of the stratum basale are living and reproduce actively; cells of the stratum corneum are dead and keratinized and form the surface layer of the skin. PART D 1. Hair root 2. Dermis 3. 4. Melanin is found in the stratum basale. It contains both elastic and collagenous fibers that give the dermis the qualities of elasticity and strength. 3. Sebaceous glands are connected to hair follicles and secrete sebum into the follicles. Hair follicles 4. PART E (sketch) Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 26 LABORATORY EXERCISE 12 BONE STRUCTURE AND CLASSIFICATION Figure Labels FIG. 12.1 1. Articular cartilage (hyaline cartilage) 2. Spongy bone (red marrow) 3. Medullary cavity 4. Yellow marrow 5. Compact bone FIG. 12.2 1. Spongy bone 2. Compact bone 3. Osteon 4. Perisoteum 5. Central canal 6. 7. 8. 9. Periosteum Proximal epiphysis Diaphysis Distal epiphysis 6. 7. 8. 9. 10. Perforating canal Blood vessels Nerve Canaliculus Osteocyte Assess: Critical Thinking Answers The closest blood supply to an osteocyte is located in the central canal of an osteon unit. Nutrients and wastes can move from one cell to another via small cellular processes located in minute tubes in the matrix called canaliculi. In this way, all of the osteocytes of one osteon are tied together to a blood source. Laboratory Report Answers PART A 1. Flat 8. 2. Short 9. 3. Long 4. Irregular 5. Sesamoid or round 6. Flat 7. Epiphysis refers to the expanded end of a long bone; diaphysis refers to the shaft between the ends of such a bone. PART B 1. Periosteum composed of irregular dense connective tissue forms the outer covering of a bone, whereas endosteum composed of reticular connective tissue lines its hollow, internal chambers. 2. Compact bone has osteons closely packed together, and spongy bone has large spaces between thin bony plates called trabeculae. . PART C (FIG. 12.5 a-b) 1. Epiphysis (distal) 2. Diaphysis 3. Epiphysis (proximal) 3. 4. 4. 5. 6. Hyaline cartilage covers the articular ends of a long bone. Dense irregular connective tissue comprises the periosteum that encloses the bone except for its articular ends. Compact bone provides strength in the shaft and along the borders of the bone. Spongy bone reduces the weight of the bone and provides spaces occupied by red marrow. The marrow of the medullary cavity of an adult is yellow, but marrow in the spaces of spongy bone is red. Medullary cavity Compact bone Spongy bone Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 27 LABORATORY EXERCISE 13 ORGANIZATION OF THE SKELETON Figure Labels FIG. 3.1a 1. Cranial bones (cranium) 5. 2. Facial bones (face) 6. 3. Skull 7. 4. Clavicle Sternum Rib Vertebral column (vertebra) 8. 9. 10. 11. Hip bone Carpals Metacarpal Phalanx 12. 13. 14. 15. FIG. 13.1b 1. Scapula 2. Humerus 3. Ulna Radius Femur Tibia 7. 8. Fibula Vertebral column (vertebra) 9. Sacrum 10. Coccyx 4. 5. 6. Patella Tarsals Matatarsal Phalanx Assess: Critical Thinking Answers The largest foramen in the skull is the foramen magnum in the occipital bone. The largest foramen in the human body is the obturator foramen in the hip bones. Laboratory Report Answers PART A 1. Axial 2. Sutural (wormian) 3. Skull 4. Hyoid 5. Coccyx 6. 7. 8. 9. 10. Sternum Twelve Pectoral Ulna Carpals 11. 12. 13. 14. Sacrum Patella Tarsals Phalanges PART B 1. c 2. e 3. 4. a d 5. 6. g b 7. f PART C 1. c 2. a 3. 4. g e 5. 6. b d 7. f PART D (FIG. 13.2) 1. 1. Hip bone 2. Sternum 3. Sacrum 4. Fibula 5. 6. 7. 8. Ulna Scapula Radius Clavicle 9. 10. 11. 12. Humerus Patella Femur Tibia 13. Rib 2. The sacrum, rib and sternum are part of the axial skeleton. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 28 LABORATORY EXERCISE 14 SKULL Instructional Suggestion You might want to have the students use colored pencils to color the bones in figure 14.2. They should use a different color for each of the individual bones in the series. This activity should cause the students to observe the figures more carefully and help them to locate the various bones that are shown from different views in the figures. The students can check their work by referring to the corresponding full-color figures in the textbook. Figure Labels FIG. 14.1 1. Parietal bone 2. Frontal bone 3. Coronal suture 4. Temporal bone 5. Perpendicular plate (of ethmoid bone) 6. Infraorbital foramen 7. Vomer 8. Mandible 9. 10. 11. 12. 13. 14. 15. 16. Supraorbital foramen Nasal bone Sphenoid bone Zygomatic bone Middle nasal concha (of ethmoid bone) Inferior nasal concha Maxilla Mental foramen FIG. 14.2 1. Parietal bone 2. Squamous suture 3. Lambdoid suture 4. Temporal bone 5. Occipital bone 6. Temporal process (of zygomatic bone) 7. External acoustic meatus 8. Mastoid process 9. Styloid process 10. Mandibular condyle 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. Zygomatic process (of temporal bone) Coronal suture Frontal bone Sphenoid bone Lacrimal bone Nasal bone Zygomatic bone Maxilla Mandible Coronoid process FIG. 14.3 1. Maxilla 2. Zygomatic bone 3. Sphenoid bone 4. Vomer 5. Zygomatic arch 6. Styloid process 7. Mastoid process 8. 9. 10. 11. 12. 13. 14. Occipital condyle Temporal bone Palatine process (of maxilla) Palatine bone Foramen magnum Lambdoid suture Occipital bone FIG. 14.4 1. Ethmoid bone 2. Foramen magnum 3. Crista galli 4. Cribriform plate (olfactory foramina) 5. Frontal bone 6. 7. 8. 9. 10. Sphenoid bone Temporal bone Sella turcica Parietal bone Occiptal bone FIG. 14.5 1. Coronal suture 2. Frontal bone 3. Sphenoid bone 4. Frontal sinus 5. Nasal bone 6. Maxilla 7. Parietal bone 8. Temporal bone 9. 10. 11. 12. 13. 14. 15. 16. Squamous suture Lambdoid suture Occipital bone Sella turcica Styloid process Sphenoidal sinus Vomer Mandible Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 29 Assess: Critical Thinking Answers The cribiform plate of the ethmoid bone with numerous olfactory foramina is a weak location of the cranium. Excessive pressure on the cribiform plate could result in a skull fracture. Laboratory Report Answers PART A 1. The frontal, parietal, occipital, temporal, sphenoid, and ethmoid cranial bones are visible on a lateral view of the skull 2. Coronal 5. Squamous 3. Sagittal 6. Frontal, ethmoid, sphenoid 4. Lambdoid 7. Maxilla PART B 1. d 2. a 3. a 4. 5. 6. f c f 7. 8. 9. f a c 10. e 11. f 12. b PART C 1. e 2. c 3. c 4. 5. 6. h d g 7. 8. 9. h a d 10. c 11. f 12. b PART D 1. c 2. a 3. 4. g f 5. 6. d b 7. 6. 7. 8. 9. Mandible Middle nasal concha (of ethmoid bone) Inferior nasal concha Mental foramen 8. 9. 10. 11. 12. 13. 14. Mandibular condyle Coronal suture Frontal bone Zygomatic process (of temporal bone) Zygomatic bone Maxilla Mandible 7. 8. 9. 10. 11. Palatine process of maxilla Palatine bone Vomer Occipital condyle Foramen magnum 5. 6. 7. 8. Ethmoid bone Sphenoid bone Sella turcica Foramen magnum e PART E (FIG. 14.10-14.13) FIG. 14.10 1. Frontal bone 2. Nasal bone 3. Zygomatic bone 4. Infraorbital foramen 5. Maxilla FIG. 14.11 1. Parietal bone 2. Squamous suture 3. Temporal bone 4. Lambdoid suture 5. Occipital bone 6. External acoustic meatus 7. Mastoid process FIG. 14.12 1. Maxilla 2. Zygomatic bone 3. Sphenoid bone 4. Temporal bone 5. Occipital bone 6. Incisive foramen FIG. 14.13 1. Frontal bone 2. Temporal bone 3. Parietal bone 4. Occiptal bone Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 30 LABORATORY EXERCISE 15 VERTEBRAL COLUMN AND THORACIC CAGE Figure Labels FIG. 15.1 1. Cervical vertebrae 2. Thoracic vertebrae 3. Lumbar vertebrae 4. Sacrum FIG. 15.2 a-b 8 3 4 7 FIG. 15.3 a-c 6 7 3 5 4 5. 6. 7. Coccyx Intervertebral foramina Intervertebral discs 5. 6. 7. 8. Sacral canal Tubercles of median sacral crest Posterior sacral foramen Sacral hiatus 1 6 5 2 1 9 2 8 FIG. 15.4 1. Superior articular process 2. Anterior sacral foramen 3. Coccyx 4. Superior articular process Assess: Critical Thinking Answers The four curvatures allow more resiliency and flexibility, which will enable the vertebal column to function more like a spring instead of a rigid rod. FIG. 15.5 1. True ribs 2. False ribs 3. Thoracic vertebra 4. Manubrium 5. Body 6. 7. 8. 9. Xiphoid process Sternum Costal cartilage Floating ribs 7. 8. 9. 10. 11. 12. Axis Dens Lumbar Five Sacral promontory Sacral hiatus Laboratory Report Answers PART A 1. Spinal cord 2. 26 3. Bodies 4. Spinal nerves 5. Vertebral arteries 6. Atlas Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 31 PART B Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 32 Vertebra Number Size Body Spinous Process Transverse Foramina Cervical 7 Smallest Smallest C2 through C6 are forked (bifid) Present Thoracic 12 Intermediate Intermediate Pointed and angled downward Absent Lumbar 5 Largest Largest Short, blunt, and nearly horizontal Absent PART C 1. Floating 2. Seven 3. Hyaline cartilage PART D (FIG. 15.7) 1. Spinous process 2. Atlas 3. Axis 4. Clavicles 5. a. Supports pectoral girdle and arms b. Protects visceral organs in thoracic and upper abdominal cavities c. Aids in breathing 4. Transverse process 5. Intervertebral disc 6. Body (of sixth cervical vertebra) Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 33 LABORATORY EXERCISE 16 PECTORAL GIRDLE AND UPPER LIMB Figure Labels FIG. 16.1 1. Clavicle 2. Rib 3. Sternum 4. Costal cartilage 5. Scapula 6. Humerus 7. 8. 9. 10. 11. Ulna Radius Acromion process Head of humerus Coracoid process FIG. 16.2a-b 1. Acromion process 2. Coracoid process 3. Spine 4. Supraspinous fossa 5. Infraspinous fossa 6. 7. 8. 9. Glenoid cavity Acromion process Coracoid process Glenoid cavity Assess: Critical Thinking Answers The clavicles brace the freely movable scapulae, helping to hold the shoulders in place. If an excessive lengthwise force occurs on this structurally weak bone, as when a person breaks a fall with an outstretched rigid upper limb, it is likely to fracture. FIG. 16.3a-b 1. Head 2. Lesser tubercle 3. Deltoid tuberosity 4. Coronoid fossa 5. Lateral epicondyle FIG. 16.4 1. Head of radius 2. Radial tuberosity 3. Styloid process of radius 4. Olecranon process FIG. 16.5 1. Olecranon process 2. Humerus 3. Olecranon fossa FIG. 16.6 1. Scaphoid 2. Capitate 3. Trapezoid 4. Trapezium 5. Carpals (carpus) 6. Metacarpals (metacarpus) 6. 7. 8. 9. 10. Capitulum Trochlea Greater tubercle Medial epicondyle Olecranon fossa 5. 6. 7. Trochlear notch Coronoid process Head of ulna 4. 5. 6. Head of radius Radius Ulna 7. 8. 9. 10. 11. Phalanges Lunate Proximal phalanx Middle phalanx Distal phalanx 5. 6. 7. 8. Spine Acromion Coracoid Head Laboratory Report Answers PART A 1. Scapulae 2. Manubrium (clavicular notch) 3. Acromion 4. Clavicle Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 34 PART B 1. a 2. b 3. b 4. 5. 6. b c d PART C (FIGS. 16.7, 16.8, and 16.9) FIG. 16.7 FIG. 16.8 1. Humerus 1. Acromion process 2. Olecranon process 2. Head of humerus 3. Head of radius 3. Clavicle 4. Radius 4. Scapula 5. Ulna 5. Rib 6. Humerus 7. 8. 9. b a b 10. e 11. a 12. f FIG. 16.9 1. Phalanges 2. Metacarpals 3. Carpals 4. Distal phalanx 5. Proximal phalanx PART D (FIG. 16.10) 6 12 5 2 9 11 4 10 1 8 7 3 PART E Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 35 LABORATORY EXERCISE 17 PELVIC GIRDLE AND LOWER LIMB Figure Labels FIG. 17.1 1. 2. Hip bone (coxal bone; pelvic bone; innominate bone) Sacrum FIG. 17.2a 1. Ilium 2. Ischial spine 3. Ischium 4. Ischial tuberosity 5. Iliac crest FIG. 17.2b 1. Anterior superior iliac spine 2. Ilium 3. Pubis 3. Coccyx 6. 7. 8. 9. Anterior superior iliac spine Acetabulum Pubis Obturator foramen 4. 5. 6. Iliac crest Ischial spine Ischium Assess: Critical Thinking Answers All of the features examined are wider in the female pelvis which will result in a larger pelvic cavity and must also serve as a birth canal for a vaginal delivery. FIG. 17.4a-b 1. Head 2. Fovea capitis 3. Greater trochanter 4. Neck 5. 6. 7. 8. Lateral epicondyle Lesser trochanter Lateral condyle Medial condyle FIG. 17.5 1. Head of fibula 2. Fibula 3. Lateral malleolus 4. Medial condyle 5. 6. 7. Tibial tuberosity Tibia Medial malleolus FIG. 17.6 1. Medial condyle 2. Femur 3. Lateral condyle 4. 5. Fibula Tibia FIG. 17.7 1. Calcaneus 2. Talus 3. Cuboid 4. Navicular 5. Lateral cuneiform 6. Intermediate cuneiform 7. Medial cuneiform 8. 9. 10. 11. 12. 13. Proximal phalanx Middle phalanx Distal phalanx Tarsals (tarsus) Metatarsals (metatarsus) Phalanges Laboratory Report Answers PART A 1. Hip bones 2. Acetabulum 3. Ilium 4. 5. 6. Ischial spines Pubic symphysis Iliac crest 7. 8. 9. Tuberosity Pubic arch Obturator foramen 10. Sacroiliac PART B 1. e 2. a 4. 5. a f 7. 8. g f 10. b 11. d Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 36 3. g 6. f PART C (FIGS. 17.8, 17.9, and 17.10) FIG. 17.8 FIG. 17.9 1. Obturator foramen 1. Lateral epicondyle 2. Pubic symphysis 2. Lateral condyle 3. Ilium 3. Head of fibula 4. Sacrum 4. Fibula 5. Head of femur 5. Femur 6. Pubis 6. Tibia PART D (FIG. 17.11) 1. Distal phalanges 2. Proximal phalanges 3. Metatarsals 4. Medial cuneiform 5. Intermediate cuneiform 6. Lateral cuneiform 9. a 12. c FIG. 17.10 1. Metatarsal 2. Proximal phalanx 3. Distal phalanx 4. Tibia 5. Talus 6. Calcaneus 7. 8. 9. 10. 11. Navicular Talus Middle phalanges Cuboid Calcaneus PART E Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 37 LABORATORY EXERCISE 18 JOINT STRUCTURE AND MOVEMENTS Assess: Critical Thinking Answers Maximum flexion of body parts can occur when in fetal position or performing a cannon ball into a swimming pool. Laboratory Report Answers PART A 1. b 2. c 3. d 4. 5. e a PART B (FIG. 18.4) 1. Fibrous (suture); synarthrosis 2. Cartilaginous (symphysis); amphiarthrosis 3. Cartilaginous (symphysis); amphiarthrosis 4. Synovial (plane); diarthrosis 5. Synovial (hinge); diarthrosis 6. 7. 8. 9. Synovial (pivot); diarthrosis Synovial (condylar); diarthrosis Synovial (saddle); diarthrosis Synovial (ball-and-socket); diarthrosis 6. 7. 8. 9. 10. a c f e e PART C 1. a 2. b 3. d 4. c 5. c PART D Table: Type of Joint Bones Included Types of Movement Possible Ball-and-socket Humerus, scapula Movements in all planes and rotation Hinge, plane, pivot Humerus, radius, ulna Flexion and extension between humerus and ulna; twisting movements occur between radius and humerus; rotation between head of radius and ulna Ball-and-socket Femur, hip bone Movements in all planes and rotation Hinge (modified), condylar, plane Femur, tibia, patella Flexion and extension between femur and tibia and slight rotation when flexed; sliding (gliding) movements occur between femur and patella PART E (FIG. 18.5) 1. Rotation 2. Elevation 3. Depression 4. Supination 5. Pronation 6. Abduction 7. Adduction 8. Flexion 9. Extension 10. Abduction 11. Adduction 12. Circumduction 13. Protraction 14. Retraction 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. Extension Flexion Extension Flexion Flexion Extension Flexion Extension Flexion Extension Flexion Extension Dorsiflexion Plantar flexion Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 38 LABORATORY EXERCISE 19 SKELETAL MUSCLE STRUCTURE AND FUNCTION Figure Labels FIG. 19.3 1. Fascicle 2. Sacroplasmic reticulum 3. Tendon 4. 5. Perimysium Nucleus Laboratory Report Answers PART A 1. l 2. i 3. c 4. 5. 6. f g b PART B (FIG. 19.5) 1. Sarcomere 2. Z line PART C 1. Origin 2. Insertion 3. 4. Triceps brachii Biceps brachii 7. 8. 9. h a j 3. 4. I band (light) A band (dark) 5. 6. Agonist Synergists 10. k 11. e 12. d 7. 8. Agonists Prime mover Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 39 LABORATORY EXERCISE 20 MUSCLES OF THE HEAD AND NECK Figure Labels FIG. 20.1 1. Temporalis 2. Occipitalis (epicranius) 3. Masseter 4. Sternocleidomastoid 5. Platysma 6. 7. 8. 9. 10. Frontalis (epicranius) Orbicularis oculi Zygomaticus major Orbicularis oris Buccinator FIG. 20.2 1. Lateral pterygoid 2. Medial pterygoid FIG. 20.3 1. Semispinalis capitis 2. Splenius capitis PART A 1. Zygomaticus major 2. Buccinator 3. Orbicularis oris 4. Lateral pterygoid 5. Temporalis 6. Medial 7. 8. 9. 10. 11. 12. Lateral Orbicularis oculi Sternocleidomastoid Splenius capitis Platysma Zygomaticus PART B 1. Epicranius 2. Zygomaticus major (or minor) 3. Masseter 4. Lateral pterygoid 5. Sternocleidomastoid 6. 7. 8. 9. 10. Buccinator Platysma Temporalis Semispinalis capitis Scalenes 4. 5. Orbicularis oris Platysma Laboratory Report Answers Assess: Critical Thinking Answers PART C (FIG. 20.4) 1. Epicranius (frontalis) 2. Zygomaticus major 3. Orbicularis oculi Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 40 LABORATORY EXERCISE 21 MUSCLES OF THE CHEST, SHOULDER, AND UPPER LIMB Figure Labels FIG. 21.1 1. 2. 3. 4. Trapezius Deltoid Latissimus dorsi Levator scapulae 5. 6. 7. 8. Supraspinatus Infraspinatus Teres minor Teres major 9. Rhomboid major 3. 4. Pectoralis major Deltoid 5. Serratus anterior 4. 5. 6. Teres minor Teres major Triceps brachii 3. 4. Biceps brachii Brachialis 3. 4. Flexor carpi radialis Palmaris longus 3. 4. Extensor carpi radialis longus Extensor digitorum 6. 7. 8. 9. 10. j a b l k 11. d 12. f 5. Teres major 6. Subscapularis 10. Brachioradialis 11. Flexor carpi radialis 12. Palmaris longus 7. 8. 9. Teres minor Brachialis Pronator teres 6. 7. 8. 9. 10. Biceps brachii Serratus anterior External oblique Deltoid Trapezius FIG. 21.2 1. 2. Pectoralis minor Internal intercostal FIG. 21.3a 1. 2. 3. Levator scapulae Supraspinatus Deltoid FIG. 21.3b 1. 2. Deltoid Subscapularis FIG. 21.4a 1. 2. Pronator teres Brachioradialis FIG. 21.4b 1. 2. Flexor carpi ulnaris Extensor carpi ulnaris Laboratory Report Answers PART A 1. 2. 3. 4. 5. c g e i h PART B 1. 2. Rhomboid major Serratus anterior 3. Pectoralis minor 4. Coracobrachialis Assess: Critical Thinking Answers PART C (FIG. 21.5a-c) 1. 2. 3. 4. 5. Trapezius Deltoid Pectoralis major Rectus abdominis Sternocleidomastoid 11. 12. 13. 14. 15. Infraspinatus Biceps brachii Triceps brachii Latissimus dorsi Pectoralis major Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 41 16. 17. 18. 19. 20. 21. Serratus anterior Biceps brachii Trapezius Deltoid Triceps brachii Brachioradialis Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 42 Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 43 LABORATORY EXERCISE 22 MUSCLES OF THE DEEP BACK, ABDOMINAL WALL, AND PELVIC FLOOR Figure Labels FIG. 22.1 1. Longissimus (intermediate group) 2. Spinalis (medial group) FIG. 22.2 1. External oblique 2. Internal oblique 3. Iliocostalis (lateral group) 3. 4. Transversus abdominis Rectus abdominis Assess: Critical Thinking Answers An appendectomy incision would involve the external oblique, internal oblique, and transversus abdominis muscles from superficial to deep. FIG. 22.3 1. Bulbospongiosus 2. Ischiocavernosus 3. 4. Superficial transversus perinei Levator ani FIG. 22.4 1. Ischiocavernosus 2. Bulbospongiosus 3. 4. Superficial transversus perinei Levator ani 4. 5. 6. Flex and rotate vertebral column Flex the vertebral column Erector spinae group 6. 7. 8. 9. 10. Ischial tuberosity Levator ani Support the pelvic viscera Ischial spine External urethral sphincter Laboratory Report Answers PART A 1. Linea alba 2. Rectus abdominis 3. Transversus abdominis PART B 1. Pelvic diaphragm (floor) 2. Close the anal canal 3. Support the pelvic viscera and provide sphincterlike action in the anal canal and vagina (aid the levator ani) 4. Bulbospongiosus 5. Constrict the vagina Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 44 LABORATORY EXERCISE 23 MUSCLES OF THE HIP AND LOWER LIMB Figure Labels FIG. 23.1 1. Tensor fasciae latae 2. Sartorius 3. Rectus femoris 4. Vastus lateralis 5. Vastus medialis 6. 7. 8. 9. Psoas major Iliacus Adductor longus Gracilis 4. 5. 6. Tensor fasciae latae Rectus femoris Vastus lateralis FIG. 23.3 1. Adductor magnus 2. Gracilis 3. Gluteus medius 4. Gluteus maximus 5. 6. 7. Biceps femoris Semitendinosus Semimembranosus FIG. 23.4 1. Fibularis (peroneus) longus 2. Tibialis anterior 3. Extensor digitorum longus FIG. 23.5 1. Gastrocnemius 2. Soleus 3. Fibularis (peroneus) longus 4. 5. 6. Tibialis anterior Extensor digitorum longus Fibularis (peroneus) brevis FIG. 23.6 1. Gastrocnemius 2. Soleus PART A 1. e 2. b 3. f 4. h 5. c 6. 7. 8. 9. 10. d j a g i PART B 1. Gluteus medius and gluteus minimus 2. Adductor magnus 3. Sartorius 4. Gastrocnemius 5. Tensor fasciae latae 6. 7. 8. 9. 10. Vastus lateralis Semitendinosus Vastus medialis Flexor digitorum longus Tibialis anterior 5. 6. 7. Tibialis anterior Gastrocnemius Soleus FIG. 23.2 1. Gluteus medius 2. Gluteus maximus 3. Biceps femoris Laboratory Report Answers Assess: Critical Thinking Answers PART C (FIG. 23.7) 1. Rectus femoris 2. Vastus medialis 3. Vastus lateralis 4. Sartorius Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 45 LABORATORY EXERCISE 24 SURFACE ANATOMY Figure Labels FIG. 24.1 1. Hyoid bone 2. Mandible 3. 4. Sternocleidomastoid Trapezius (upper) FIG. 24.3a-b 1. Sternum 2. Sternocleidomastoid 3. 4. Biceps brachii Serratus anterior FIG. 24.4 1. Acromion process 2. Olecranon process 3. Lateral malleolus 3. 4. Calcaneal tendon Gastrocnemius FIG. 24.6 1. Medial malleolus 2. Patella FIG. 24.8 1. Lateral malleolus 2. Patella Table 24.1 Representative Bony and Soft Tissue Surface Features Bony and Soft Tissues Bony Features Soft Tissue Features Biceps brachii X Calcaneal tendon X Deltoid X Gastrocnemius X Greater trochanter X Head of fibula X Iliac crest X Iliotibial tract X Lateral malleolus X Mandible X Mastoid process X Medial border of scapula X Rectus abdominis X Sacrum X Serratus anterior X Sternocleidomastoid X Sternum X Thenar eminence X Tibialis anterior X Zygomatic arch Laboratory Report Answers Part A 1. b 4. 2. d 5. X d b 7. 8. c b 10. a 11. a 13. d 14. c Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 46 3. b Part B FIG. 24.9a 1. Zygomatic arch 2. Trapezius 3. Clavicle 4. Xiphoid process 5. Rectus abdominis 6. Iliac crest 7. Greater trochanter 8. Tibial tuberosity 9. Tibia 10. Acromion process FIG. 24.9a (continued) 11. Pectoralis major 12. Biceps brachii 13. Thenar eminence 14. Vastus lateralis 15. Head of fibula 6. a 9. c 12. c FIG. 24.9b 1. Mastoid process 2. Erector spinae 3. Iliac crest 4. Styloid process of ulna 5. Iliotibial tract 6. Popliteal fossa 7. External occipital protuberance 8. C7 9. Medial border of scapula 10. Inferior angle of scapula FIG. 24.9b (continued) 11. Olecranon process 12. Sacrum 13. Distal interphangeal joint 14. Soleus 15. Calcaneal tendon Part C (FIG. 24.10) Answers will vary. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 47 LABORATORY EXERCISE 25 NERVOUS TISSUE AND NERVES Figure Labels FIG. 25.1 1. Nucleus 2. Nucleolus 3. Nodes of Ranvier 4. 5. 6. Schwann cell Dendrites Cell body FIG. 25.2 1. Neurilemma (of Schwann cell) 2. Myelin sheath (of Schwann cell) 3. 4. Axon (nerve fiber) Schwann cell nucleus Laboratory Report Answers PART A 1. g 2. c 3. 4. h f 5. 6. e d 7. 8. a b PART B 1. d 2. f 3. 4. h g 5. 6. e a 7. 8. c b PART C (sketches) PART D (sketches) Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 48 LABORATORY EXERCISE 26 BRAIN AND CRANIAL NERVES Figure Labels FIG. 26.1 12 1 10 4 8 3 FIG. 26.2 1. Frontal lobe 2. Insula 3. Temporal lobe 6 11 5 7 9 2 4. 5. Parietal lobe Occipital lobe FIG. 26.3 1. Motor area for voluntary muscle control 2. Motor speech area (Broca’s area) 3. Auditory area 4. 5. 6. Cutaneous sensory area Sensory speech area (Wernicke's area) Visual area FIG. 26.4 1. (I) Olfactory nerve 2. (II) Optic nerve 3. (III) Oculomotor nerve 4. (IV) Trochlear nerve 5. (V) Trigeminal nerve 6. (VI) Abducens nerve 7. 8. 9. 10. 11. 12. (VII) Facial nerve (VIII) Vestibulocochlear nerve (IX) Glossopharyngeal nerve (X) Vagus nerve (XI) Accessory nerve (XII) Hypoglossal nerve PART A 1. j 2. f 3. k 4. c 5. e 6. a 7. 8. 9. 10. 11. 12. i l h d g b PART B (FIG. 26.5) 1. Corpus callosum 2. Thalamus 3. Hypothalamus 4. Diencephalon 5. Midbrain 6. 7. 8. 9. 10. Pons Medulla oblongata Brainstem Cerebrum Cerebellum PART C 1. k 2. l 3. j 4. i 5. f 6. g 7. 8. 9. 10. 11. 12. b e h a c d Laboratory Report Answers Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 49 LABORATORY EXERCISE 27 DISSECTION OF THE SHEEP BRAIN Instructional Suggestion Rather than have students dissect sheep brains, you might want to provide the class with samples of whole sheep brains and sectioned brains for examination. This should extend the use of the available specimens. Laboratory Report Answers PART A 1. The brain would have the delicate pia mater adhering to its outer surface of the gyri and sulci. The arachnoid mater spans the fissures of the brain. The dura mater of the meninges probably remained adhering to the inside of the skull when the brain was removed. 2. The human cerebral hemispheres are relatively larger than those of the sheep. 3. There are more gyri and sulci in the human cerebrum. 4. The human cerebrum with its larger size and greater number of gyri is more complex and thus able to carry on more complex functions. 5. The human cerebellum is divided in the midline (vermis) into two hemispheres, whereas the sheep cerebellum is not divided. 6. The olfactory bulbs of the sheep brain are larger than those of the human brain. 7. The olfactory, optic, and trigeminal nerves seem to be most highly developed in the sheep brain. 8. The senses of smell and sight and the sensory functions associated with the trigeminal nerve are highly developed. Assess: Critical Thinking Answers PART B 1-6. Answers will vary. The sheep brain and the human brain features are more similar than different. Therefore a complete list of similar features would be very long. Among similar features include two cerebral hemispheres, medulla oblongata, pineal gland, midbrain, thalamus, hypothalamus, pons, olfactory bulb, four ventricles, and others. (Note only 6 answers are needed.) Mammal brains have more similarities than differences. PART C (FIG. 27.6) 4 1 2 8 7 6 3 9 5 Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 50 LABORATORY EXERCISE 28 SPINAL CORD AND MENINGES Figure Labels FIG. 28.1 1. Subarachnoid space 2. White matter 3. Posterior root of spinal nerve 4. Anterior root of spinal nerve 5. 6. 7. Body of vertebra Epidural space Gray matter FIG. 28.2 1. Posterior horn 2. Lateral funiculus 3. Anterior horn 4. Central canal 5. 6. 7. Gray commissure Anterior median fissure Anterior funiculus 6. 7. 8. 9. 10. Anterior Gray commissure Central canal Funiculi Nerve tracts 4. 5. d c FIG. 28.5 1. Pia mater 2. Arachnoid mater 3. Dura mater Laboratory Report Answers PART A 1. Spinal nerves 2. Cervical enlargement 3. Lumbar enlargement 4. Posterior median sulcus 5. Horns PART B 1. b 2. e 3. a PART C (FIG. 28.6) 8 4 5 1 6 3 2 7 PART D 1. b 2. c 3. 4. d f 5. 6. g a 7. e Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 51 LABORATORY EXERCISE 29 REFLEX ARC AND REFLEXES Laboratory Report Answers PART A 1. Pathways 2. Central nervous system (spinal cord) 3. Reflexes 4. Muscles 5. Sensory 6. Quadriceps femoris 7. 8. 9. 10. 11. 12. Quadriceps femoris Posture Skin Flexor Flexion Babinski PART B 1. Table: (Note: The answers will vary in relation to the degree of the response.) Response Observed Effector Involved Extension of leg at the knee joint Quadriceps femoris Plantar flexion of the foot Gastrocnemius and soleus Flexion of forearm at the elbow joint or slight biceps twitch Biceps brachii Extension of forearm at the elbow joint or slight triceps twitch Triceps brachii Plantar flexion of the foot and flexion of toes 2. Gastrocnemius, soleus, and flexor digitorum longus The quadriceps femoris is stretched, stimulating stretch receptors (muscle spindles) within the muscle. As a result, impulses pass along sensory neurons into the spinal cord and synapse with a motor neuron. Motor impulses travel out of the cord on nerve fibers that lead to the quadriceps femoris. Muscle fibers contract, and the leg is extended at the knee joint. Assess: Critical Thinking Answers All of these reflexes are rapid, subconscious responses to physical stimuli. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 52 LABORATORY EXERCISE 30 RECEPTORS AND GENERAL SENSES Laboratory Report Answers PART A 1. Chemoreceptors 2. Pain 3. Thermoreceptors 4. Photoreceptors 5. Sensory adaptation PART B 1. (experimental results) 2. (experimental results) PART C 1. (experimental results) PART D 1. (experimental results) 2. (experimental results) 6. 7. 8. 9. 10. Touch Pressure 25ºC (77ºF) and 45ºC (113ºF) 10ºC (50ºF) and 20ºC (68ºF) General 3. Answers will vary. 2. Answers will vary. 3. Answers will vary. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 53 LABORATORY EXERCISE 31 SMELL AND TASTE Figure Labels FIG. 31.1 6 5 3 7 FIG. 31.3 6 1 4 1 2 4 3 5 2 Laboratory Report Answers PART A 1. Bipolar 2. Cilia 3. Water 4. Cribriform plate 5. Crista galli PART B 1. (experimental results) 2. (experimental results) 6. 7. 8. 9. 10. Olfactory tracts Frontal Sensory adaptation Outside environment Odorless 3. Answers will vary. Assess: Critical Thinking Answers Answers will vary. Any variations in time for sensory adaptation could be a result of the concentration of the test substance, volatility of the test substance, or the number of receptors for the test substance. PART C 1. 2. 3. 4. Epithelial Taste pore Taste hairs (microvilli) Water 5. 6. 7. 8. Receptor Acids Salts Bitter PART D 1. (experimental results) 2 (a-c). Answers will vary. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 54 LABORATORY EXERCISE 32 EAR AND HEARING Figure Labels FIG. 32.1 1. Auricle 2. Malleus 3. Incus 4. Semicircular canals 5. Stapes 6. Cochlea FIG. 32.2 4 6 7. 8. 9. 10. 11. 5 3 Vestibulocochlear nerve Oval window Tympanic membrane (eardrum) Auditory tube External acoustic meatus 1 2 Assess: Critical Thinking Answers The largest ear structure is the auricle which is able to trap and funnel a minute sound wave into the middle and inner ear structures. This will allow a concentration of the vibrations making the sound detection more likely to occur. Laboratory Report Answers PART A 1. e 2. k 3. j 4. 5. 6. PART B (FIG. 32.8) 1. Cochlear duct 2. Tectorial membrane 3. Hair cells (receptor cells) PART C 1. (experimental results) 2. (experimental results) h d c 7. 8. 9. l i f 4. 5. Basilar membrane Scala tympani 3. 4. (experimental results) Answers will vary. 10. b 11. a 12. g Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 55 LABORATORY EXERCISE 33 EAR AND EQUILIBRIUM Laboratory Report Answers PART A 1. Utricle 2. Temporal 3. Macula 4. Calcium carbonate 5. Vestibulocochlear (vestibular branch) 6. 7. 8. 9. 10. Ampulla Crista ampullaris Cupula Inertia Cerebellum PART B 1. a. The eyes, inner ears, and proprioceptors provide information needed to maintain equilibrium when the eyes are open. b. The inner ears and proprioceptors provide such information when the eyes are closed. 2. a. (experimental results) b. Probably yes; this demonstrates the importance of visual information in maintaining equilibrium. c. With the eyes closed, such a person would receive very little sensory information needed to maintain equilibrium. 3. a. (experimental results) b. Answers will vary. Assess: Critical Thinking Answers Vision, touch, and proprioception would all supplement equilibrium. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 56 LABORATORY EXERCISE 34 EYE STRUCTURE Figure Labels FIG. 34.1 1. 2. Lacrimal gland Superior and inferior canaliculi 3. 4. Lacrimal sac Nasolacrimal duct 3. 4. Medial rectus Lateral rectus 5. 6. Inferior rectus Inferior oblique 7. 8. 9. 10. 11. 12. Aqueous humor Anterior cavity Retina Choroid coat Sclera Vitreous humor 13. 14. 15. 16. Fovea centralis Optic nerve Optic disc Posterior cavity FIG. 34.2 1. 2. Superior oblique Superior rectus FIG. 34.3 1. 2. 3. 4. 5. 6. Pupil Iris Ciliary body Suspensory ligaments Lens Cornea Assess: Critical Thinking Answers The delicate retina is only located next to the choroid coat by the pressure maintained by the vitreous humor. Any alteration of this pressure could allow the retina to detach as was easily observed during the dissection. No connective tissue was observed between the inner and middle tunics of the eye. Laboratory Report Answers PART A 1. 2. 3. 4. 5. b j e d g 6. 7. l h 8. 9. 10. 11. 12. 13. f a i k c Cornea, aqueous humor, pupil of iris, lens, vitreous humor, retina 14. More rods than cones; rods stimulated by dim light; cones enable perception of color [other answers could be acceptable.] 4. Aqueous humor occurs between the cornea and the lens. The dark pigment absorbs excess light and keeps the eye dark inside. 6. PART B 1. 2. 3. The outer tunic/layer (sclera) is toughest. Dense (fibrous) connective tissue. The pupil of the dissected eye probably was elliptical in shape, and the human pupil is round. 5. 7. The lens is biconvex, flexible, and transparent. It may be firm and opaque in a preserved eye. The vitreous humor is a transparent, jellylike fluid. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 57 PART C Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 58 LABORATORY EXERCISE 35 VISUAL TESTS AND DEMONSTRATIONS Assess: Critical Thinking Answers When using both eyes for observations, if the image of a small object falls on the optic disc of one eye, the object is still seen by the other eye. This can be confirmed because the blind-spot demonstration will not work with both eyes open. Laboratory Report Answers PART A 1. (experimental results) 2. (experimental results) 3. (experimental results) 4. (experimental results) 5. a. A person with 20/70 vision can see from 20 feet what the normal eye sees from 70 feet. This person has less than normal vision. b. A person with 20/10 vision can see from 20 feet what the normal eye sees from 10 feet. This person has better than normal vision. c. Astigmatism results in blurred vision because some parts of the image on the retina are in focus, while other parts are not in focus. d. The elastic quality of the lens tends to decrease with age. e. The retina is lacking cones that are sensitive to red or green wave lengths (an X-linked/sex-linked trait). PART B 1. (experimental results) 2. a. The optic disc lacks photoreceptors (rods and cones) and thus creates a blind spot in the retina. b. The photopupillary reflex involves the constriction of the pupil in response to exposure to bright light. c. The photopupillary reflex occurs in both eyes even when one eye is shielded from the light; however, the shielded eye may not show as much change as the exposed one. d. When an eye is focused on a close object, the pupil constricts. e. When the eyes are focused on a close object, they converge toward the midline. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 59 LABORATORY EXERCISE 36 ENDOCRINE HISTOLOGY AND DIABETIC PHYSIOLOGY Instructional Suggestions 1. You might consider the live fish part of this lab as a class demonstration to save expenses and materials needed. 2. HumulinR administered in 200 units will suffice to induce insulin shock within about 5 minutes. 3. Consult with your local pharmacist to purchase insulin and insulin syringes. Store the HumulinR in the refrigerator – do not freeze. 4. The recovery from insulin shock when the fish is placed into a 10% solution of glucose is usually within 5 minutes. 5. The recovery from insulin shock works faster if the fish selected is very small (total length about 1-1.5 inches). The species of fish selected does not seem significant so try a fish species that is readily available. It does not work well to use the same fish for a second experiment for the next section of class. The fish usually survive the experiment and a student is almost always willing to take the fish home after the class is over. 6. It probably works to use the same beakers of insulin and glucose solution for other sections of class the same day; however, use a different fish. Be sure to rinse the fish net between fish transfers from one solution to another and the holding container of aquarium water. Figure Labels FIG. 36.1 1. 2. 3. 4. 5. Hypothalamus Pituitary gland Testis (male) Pineal gland Thyroid gland 6. 7. 8. 9. Thymus Adrenal gland Pancreas Ovary (female) Laboratory Report Answers PART A 1. Growth hormone, thyroid-stimulating hormone (TSH), adrenocorticotropic hormone (ACTH), follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin. 2. Antidiuretic hormone (ADH), oxytocin 3. a. antidiuretic hormone b. growth hormone c. thyroid-stimulating hormone d. oxytocin e. adrenocorticotropic hormone` f. prolactin 4. Thyroxine (T4), triiodothyronine (T3) 5. Calcitonin 6. Parathyroid hormone 7. Bones, intestine, kidneys 8. Epinephrine, norepinephrine 9. Increased heart rate, increased blood pressure, rise in blood sugar concentration, increased metabolic rate, increased breathing rate, dilation of airways, decreased activity in the digestive tract (These are seven possible responses for five requested.) 10. Aldosterone 11. Kidneys conserve sodium ions, kidneys increase excretion of potassium ions, kidneys conserve water (reduce urine volume). (These are three possible responses for two requested.) 12. Cortisol 13. Decreases protein synthesis, increased release and use of fatty acids, stimulates liver to produce glucose from noncarbohydrates 14. The alpha cells secrete glucagon; the beta cells secrete insulin. Assess: Critical Thinking Answers Glucagon stimulates change of glycogen to glucose, causing an increase in blood glucose concentration. Insulin causes a decrease in the blood glucose concentration by promoting the transport of glucose into cells. PART B (sketches) PART C Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 60 1. 2. 3. 4. 5. f d h e a 6. 7. 8. c b g PART D Table: Characteristic Type 1 Diabetes Type 2 Diabetes Onset age Early age or adult Usually over 40 Onset of symptoms Rapid Slow Percentage of diabetics 10-15% 85-90% Natural insulin levels Below normal Normal or increased Beta cells of pancreatic islets Destroyed Not Destroyed Pancreatic islet cell antibodies Present Absent Risk factors of having the disease Heredity Heredity, obesity, lack of exercise Typical treatments Insulin administration Avoid foods that stimulate insulin production, weight control, exercise, medications Untreated blood sugar levels Hyperglycemia Hyperglycemia PART E 1. (experimental results) 2. (experimental results) The amount of time until insulin shock occurs varies with the type of insulin used and the size of the fish selected for the experiment. Rapid or irregular swimming, gill cover, and mouth movements usually are noted within about 5-10 minutes. 3. (experimental results) Recovery from insulin shock usually occurs within about 5-10 minutes. Assess: Critical Thinking Answers The main hormone regulating blood sugar is insulin. A normal person’s insulin level will adjust the blood sugar homeostasis level according to changes in daily diets and exercise levels. A person with type 1 diabetes needs to attempt to have similar daily diets and exercise to correlate with the designated daily insulin administered. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 61 LABORATORY EXERCISE 37 BLOOD CELLS AND BLOOD TYPING Warning Because of the possibility of blood-borne infections being transmitted from one student to another if blood slides are prepared in the classroom, it is suggested that commercially prepared blood slides and commercially prepared simulated blood-typing kits be used in this exercise. The instructor, however, may wish to demonstrate the procedures. Laboratory Report Answers PART A 1. Erythrocytes 2. Transporting and exchanging gases (O2 and CO2) 3. Hemoglobin 4. Leukocytes 5. 6. 7. 8. Granulocytes Agranulocytes Neutrophils Nucleus PART C 1. (experimental results) 2. Answers will vary. PART D 1. Membrane 2. Four 3. A 4. B 5. 6. 7. 8. Anti-B Anti-A AB O PART E 1. (experimental results) 2. Answers will vary. 3. Answers will vary 4. 5. Answers will vary. Answers will vary. PART F 1. Rhesus monkey 2. Antigen D 3. Rh-negative 4. 5. Agglutinate Rh-positive 4. Answers will vary. PART B (sketches) PART G 1. 2. 3. (demonstration results) Answers will vary. Answers will vary. PART H 1. A 2. Rh-positive Assess: Critical Thinking Answers 1. 2. The anti-A serum would contain anti-A antibodies if clumping was observed for a person with type A blood. The anti-B serum would contain anti-B antibodies if clumping was observed for a person with type B blood. A total white blood cell count provides the number of white blood cells in a given volume of blood; a differential white blood cell count gives the relative percentages of types of white blood cells in a blood sample. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 62 LABORATORY EXERCISE 38 HEART STRUCTURE Instructional Suggestions 1. 2. You may want to have the students use colored pencils to color the features of the heart and blood vessels in figure 38.3. This activity should help them observe the illustrations more carefully and locate the various features shown from different views in the figures. They can check their work by referring to the corresponding figures in the textbook, which are presented in full color. Instead of using preserved sheep hearts, you might want to provide fresh pig hearts for dissection. 3. 4. To reduce the cost of specimens used, you might provide predissected, preserved sheep hearts for observation and save the specimens for use with other classes. Fresh beef hearts are sometimes available from meatpacking houses. You might want to demonstrate the dissection of this large heart. Try to make sure that the atria and large blood vessels are left attached for this purpose. Figure Labels FIG. 38.1 1. Aorta 2. Superior vena cava 3. Right atrium 4. Right coronary artery 5. Right ventricle 6. Inferior vena cava 7. 8. 9. 10. Pulmonary trunk (artery) Left coronary artery Great cardiac vein Left ventricle 6. 7. 8. 9. 10. Apex Superior vena cava Right atrium Inferior vena cava Right ventricle FIG. 38.3 1. Aorta 2. Superior vena cava 3. Aortic valve 4. Right atrium 5. Tricuspid valve 6. Chordae tendineae 7. Inferior vena cava 8. Left pulmonary artery 9. Pulmonary trunk 10. 11. 12. 13. 14. 15. 16. 17. Left pulmonary veins Left atrium Pulmonary valve Mitral (bicuspid) valve Papillary muscle Interventricular septum Left ventricle Right ventricle FIG. 38.4 1. Superior vena cava 2. Aorta 3. Right atrium 4. Aortic valve 5. Tricuspid valve 6. Right ventricle 7. 8. 9. 10. 11. Interventricular septum Left atrium Mitral valve Left ventricle Apex 7. 8. 9. 10. 11. 12. e g i b c a FIG. 38.2 1. Aorta 2. Left pulmonary artery 3. Left pulmonary veins 4. Left atrium 5. Left ventricle Laboratory Report Answers PART A 1. h 2. l 3. j 4. f 5. k 6. d Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 63 PART B 1. The right atrioventricular valve is composed of three relatively large cusps that contain chordae tendineae; the pulmonary valve is made up of three smaller pocket-like cusps that lack chordae tendineae. 4. 2. The cusps of the right atrioventricular valve move upward into a horizontal position and close the opening between the right atrium and the right ventricle. The chordae tendineae and papillary muscles prevent the cusps of the right and left atrioventricular valves from swinging into the atria when the ventricles contract. 5. 3. The thicker wall of the aorta allows it to withstand the higher pressure of the blood pumped out from the left ventricle. The thinner wall of the pulmonary trunk (artery) is related to the lower pressure of the blood that leaves the right ventricle. Vena cava, right atrium, right atrioventricular (tricuspid) valve, right ventricle, pulmonary valve, pulmonary trunk, the lungs, pulmonary vein, left atrium, left atrioventricular (mitral; bicuspid) valve, left ventricle, aortic valve, aorta. Assess: Critical Thinking Answers The thicker wall of the left ventricle allows it to contract with greater force and create the high pressure needed to move blood to all parts of the body (systemic circuit) except the lungs. The thinner wall of the right ventricle creates the lower pressure needed to move blood a relatively short distance to the lungs (pulmonary circuit). Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 64 LABORATORY EXERCISE 39 CARDIAC CYCLE Laboratory Report Answers PART A 1. 70 2. Systole 3. Diastole 4. Closed 5. Open 6. 7. 8. 9. 10. Ventricles Vibrations AV valves Pulmonary and aortic valves (semilunar valves) Murmur PART B 1. (experimental results) 2. (experimental results) PART C 1. Cardiac muscle 2. SA (sinoatrial; sinu-atrial) 3. AV (atrioventricular) 4. AV bundle (bundle of His) 5. Purkinje 6. Electrocardiogram 7. Polarized 8. Atria PART D 1. (labeled ECG recordings) 2. Answers will vary. 3. Normal is 0.12-0.20 sec. 4. The PQ (PR) interval indicates the time it takes for the atria to depolarize and the cardiac impulse to reach the AV node. 9. Ventricles 10. Ventricles 11. Atrial repolarization occurs at the same time that the ventricular fibers depolarize. The QRS complex indicating ventricular depolarization obscures any recording of the atrial repolarization. 12. Tachycardia; bradycardia 5. 6. Because each QRS wave in the pattern indicates a ventricular contraction, the heart rate can be determined by counting the QRS waves that occur in a minute. (experimental results) Assess: Critical Thinking Answer 36 Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 65 LABORATORY EXERCISE 40 BLOOD VESSEL STRUCTURE, ARTERIES, AND VEINS Instructional Suggestions If live frogs are not available for the microscopic observation of blood vessels, you might want to provide small goldfish. The head of a fish can be wrapped loosely in wet cotton to keep its gills moist, and the fish can be placed on a glass plate on the stage of a microscope. If its tail is spread out beneath the microscope slide, the blood vessels can be observed with low- and highpower magnification. However, if the fish is not returned to water within a few minutes, it will likely die. Figure Labels FIG. 40.1 1. Tunica interna 2. Tunica media FIG. 40.4 1. Superior vena cava 2. Pulmonary trunk 3. Tunica externa 3. 4. Inferior vena cava Pulmonary veins 5. 6. Pulmonary artery Aorta Assess: Critical Thinking Answer The left ventricle wall is thicker which provides a more powerful contraction to force the blood through the longer distance of the systemic circuit. FIG. 40.5 1. 2. 3. 4. Right common carotid artery Right subclavian artery Brachiocephalic artery Aortic arch 5. 6. 7. 8. Ascending aorta Right renal artery Right common iliac artery Left common carotid artery 9. Left subclavian artery 10. Coronary artery (left) 11. Abdominal aorta 4. 5. 6. Vertebral artery Subclavian artery Facial artery 7. 8. Common carotid artery Brachiocephalic artery 4. 5. 6. Brachial artery Radial artery Ulnar artery 4. 5. 6. Popliteal artery Abdominal aorta Internal iliac artery 7. 8. 9. Femoral artery Anterior tibial artery Dorsalis pedis artery 3. 4. Internal jugular vein Vertebral vein 5. Brachiocephalic vein 2. 3. Cephalic vein Basilic vein 4. Median cubital vein 3. 4. Gastric vein (right) Splenic vein 5. Inferior mesenteric vein FIG. 40.6 1. 2. 3. Superficial temporal artery Internal carotid artery External carotid artery FIG. 40.8 1. 2. 3. Subclavian artery Axillary artery Deep brachial artery FIG. 40.9 1. 2. 3. Common iliac artery External iliac artery Deep femoral artery FIG. 40.10 1. 2. External jugular vein Subclavian vein FIG. 40.11 1. Axillary vein FIG. 40.12 1. 2. Hepatic portal vein Superior mesenteric vein FIG. 40.13 Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 66 1. 2. 3. Inferior vena cava Common iliac vein Internal iliac vein 4. 5. 6. External iliac vein Femoral vein Great saphenous vein Laboratory Report Answers PART A 1. Endothelium 2. Middle layer (tunica media) 3. 4. 5. Outer layer (tunica externa) Vasoconstriction Vasodilation 6. 7. 8. 9. Capillaries Hydrostatic Osmotic Valves PART B 1. (sketches) 2. The inner and outer layers are similar in the artery and vein. The middle layer of the artery contains relatively greater amounts of smooth muscle and elastic tissue than that of the vein. Assess: Critical Thinking Answer Because arteries are under higher pressure than veins, the thicker arterial walls help to maintain the strength and elasticity necessary against their walls. PART C 1. The blood in an arteriole moves with a pulsating rapid flow, but blood in a venule moves with a steady slower flow. 2. A capillary could be identified by its small diameter and the presence of blood cells moving in single file. 3. Blood moves fastest in arterioles, somewhat slower in venules, and slowest in capillaries. PART D 1. 2. 3. 4. d i c a 5. 6. 7. 8. f b h j 9. g 10. e 4. 5. 6. e h c 7. 8. PART E 1. 2. 3. a b d g f PART F Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 67 PART G (FIG. 40.14) 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. Common carotid artery Brachiocephalic vein Superior vena cava Femoral vein Great saphenous vein External jugular vein Subclavian artery Axillary vein Pulmonary vein Inferior vena cava Abdominal aorta Common iliac vein Common iliac artery Femoral artery Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 68 Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 69 LABORATORY EXERCISE 41 PULSE RATE AND BLOOD PRESSURE Instructional Suggestions The following suggestions should be considered when trying to obtain an accurate blood pressure: 1. 2. 3. The room environment should have a moderate temperature and be quiet (no talking). The client needs to be relaxed and comfortable. A temporary increase in blood pressure could exist from smoking, pain, anxiety, or a full urinary bladder. Palpate the pulse first so that you are certain to pump the cuff high enough to not miss the first tapping sound. It also assures that you do not pump the cuff so high that we alter the blood pressure when releasing air. Laboratory Report Answers PART A 1. Arterial 2. Systolic 3. Diastolic 4. Heart 5. Pressure 6. 7. 8. 9. 10. Sphygmomanometer Millimeters of mercury (mm Hg) Systolic Pulse pressure Brachial PART B 1. (test results) 2. Answers will vary. PART C 1. (test results) 2. (test results) 3. 4. Answers will vary. Answers will vary. Assess: Critical Thinking Answer A palpated pulse would be characteristic of the systolic pressure as the arterial wall is expanding at that moment under the higher pressure. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 70 LABORATORY EXERCISE 42 LYMPHATIC SYSTEM Figure Labels FIG. 42.1 5 3 2 4 FIG. 42.2 1 2 4 7 1 6 6 3 5 Laboratory Report Answers PART A 1. Lymphatic capillaries 2. Squamous epithelial 3. Lymph 4. Veins 5. Valves 6. 7. 8. 9. Nodes Intestinal Jugular Thoracic (left lymphatic) PART B 1. Lymphocytes 2. Hilum 3. Nodules 4. Sinuses 5. Afferent 6. 7. 8. 9. Capsule Peyer’s patches Scalp, face, nasal cavity, and pharynx Inguinal PART C 1. Mediastinum 2. Puberty 3. Adipose and connective tissues 4. Bone marrow 5. Thymosins 6. Spleen 7. 8. 9. 10. 11. Hilum Blood White Red Macrophages PART D (sketches) Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 71 LABORATORY EXERCISE 43 DIGESTIVE ORGANS Figure Labels FIG. 43.1 1. Lip 2. Hard palate 3. Soft palate 4. Uvula 5. 6. 7. Palatine tonsil Tongue Vestibule FIG. 43.2 1. Parotid gland 2. Masseter muscle 3. Tongue 4. 5. Sublingual gland Submandibular gland FIG. 43.3 1. Enamel 2. Dentin 3. Root 4. 5. 6. Crown Gingiva Root canal FIG. 43.5 1. Pharyngeal tonsils (adenoids) 2. Opening of auditory tube (Eustachian tube) 3. Nasopharynx 4. Palatine tonsils 5. 6. 7. 8. Oropharynx Lingual tonsils Epiglottis Laryngopharynx 6. 7. 8. Fundus Cardia Body 5. 6. 7. Bile duct Pancreatic duct Hepatopancreatic sphincter (sphincter of Oddi) FIG. 43.7 1. Esophagus 2. Gastric folds (rugae) 3. Pyloric sphincter 4. Duodenum 5. Lower esophageal sphincter (cardiac sphincter) FIG. 43.9 1. Cystic duct 2. Gallbladder 3. Duodenum 4. Hepatic duct (common) FIG. 43.12 10 4 1 3 7 2 6 9 8 5 Assess: Critical Thinking Answer The small intestine, which is much longer than the large intestine and contains villi, provides more surface area for absorption than the large intestine. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 72 Laboratory Report Answers PART A 1. b 2. i 3. e 4. 5. 6. f g j PART B 1. Nasopharynx 2. Oropharynx 3. Laryngopharynx 4. Mucus PART C 1. Cardia, fundus, body, and pylorus (pyloric part) 2. Chief cells 3. Parietal cells 4. Pepsin 7. 8. 9. h c k 10. d 11. a 5. The esophagus provides a passageway for food from the pharynx to the stomach . 5. 6. Chyme The stomach receives food from the esophagus, mixes it with gastric juice, initiates the digestion of protein, does limited amount of absorption, and moves food (chyme) into the small intestine. PART D (sketch) PART E 1. Duodenum, jejunum, ileum 4. 2. A mesentery supports and suspends organs. It 5. contains blood vessels, lymphatic vessels, and nerves that supply the organs. 3. Peptidases, sucrase, maltase, lactase, intestinal lipase, 6. enterokinase (only 5 of 6 needed to answer the question) Ileocecal sphincter (valve) The small intestine receives secretions from the pancreas and liver, completes digestion of nutrients, absorbs the products of digestion, and transports the residues to the large intestine. The large intestine absorbs water and electrolytes, and forms and stores feces. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 73 LABORATORY EXERCISE 44 ACTION OF A DIGESTIVE ENZYME Instructional Suggestion For this experiment to work the best, it is important to obtain amylase that is free of sugar. Some of the amylase sold by laboratory suppliers contains sugar, as can be determined by the control in tube 1 of this experiment. A low maltose solution of amylase yields good results. Keep any of the unused amylase frozen. The alternative procedure using human saliva does not contain sugar and works well for this experiment. Use proper safety procedures when using human body fluids. Laboratory Report Answers PART A 1. Expected experimental results: Tube Starch Sugar 1 0 0 2 + 0 3 (varies) + 2. a. Testing the amylase solution for the presence of starch and sugar demonstrates the negative results of the tests. b. Tube 2 demonstrates that starch will not change to sugar when warmed to 37ºC (98.6ºF). c. The change of starch to sugar is a result of the action of the amylase in tube 3. PART B 1. Expected experimental results: Tube Starch Sugar 4 + (varies) 5 (varies) + 6 + 0 2. a. Amylase is slow to act or inactive in low temperature and high temperature. Its optimum temperature is near 37ºC (98.6ºF). b. The tubes in which digestion failed to occur could be placed in the 37ºC (98.6ºF) water bath. If digestion occurred at this temperature, the enzyme was not destroyed by the previous treatment. Assess: Critical Thinking Answers The test would show a positive result for sugar. The experiment would not be valid as it would not show a change from starch to sugar when sugar is already present. (Note: some amylase sold contains sugar.) Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 74 LABORATORY EXERCISE 45 RESPIRATORY ORGANS Figure Labels FIG. 45.1 1. Nostril 2. Oral cavity 3. Epiglottis 4. Larynx 5. Bronchus (right main) 6. Right lung 7. 8. 9. 10. 11. Frontal sinus Nasal cavity Pharynx Trachea Left lung FIG. 45.2 1. Frontal sinus 2. Nostril 3. Auditory tube opening 4. Uvula 5. Palatine tonsil 6. Epiglottis 7. Trachea 8. 9. 10. 11. 12. 13. 14. Superior nasal concha Middle nasal concha Inferior nasal concha Sphenoidal sinus Nasopharynx Oropharynx Laryngopharynx FIG. 45.3 1. Epiglottic cartilage (epiglottis) 2. Thyroid cartilage 3. Cricoid cartilage 4. 5. 6. Epiglottic cartilage (epiglottis) Thyroid cartilage Cricoid cartilage FIG. 45.4 1. Epiglottis 2. Glottis 3. 4. False vocal cord (vestibular fold) True vocal cord (vocal fold) 7. 8. 9. j f g Laboratory Report Answers PART A 1. h 2. b 3. i 4. 5. 6. a e d 10. c PART B (sketches) PART C 1. The sticky mucus is secreted into the upper and 3. lower respiratory tract, which will trap particles of dust and microorganisms. 4. 2. The cilia create a current of mucus toward the pharynx. The mucus contains entrapped particles that are usually swallowed. If the smooth muscle of the bronchial tree relaxes, the air passages dilate, which allows a greater volume of air movement. If the smooth muscle of the bronchial tree contracts, the air passages constrict the size of the lumens, which reduces the volume of air movement. Assess: Critical Thinking Answer The simple squamous epithelial cells allow for rapid diffusion of oxygen and carbon dioxide between the blood and the alveolar air. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 75 LABORATORY EXERCISE 46 BREATHING AND RESPIRATORY VOLUMES Assess: Critical Thinking Answer Aging results in some natural loss of elasticity of the lungs as well as the muscles (diaphragm and intercostal muscles) used in breathing. This can be measured by a vital capacity test. Laboratory Report Answers PART A 1. Ventilation 2. Atmospheric 3. 760 4. Atmospheric pressure 5. Phrenic 6. Increases PART B 1. a 2. g 3. e 4. f 7. 8. 9. 10. 11. 12. 5. 6. 7. 8. External intercostal Visceral pleura Surfactant Elastic recoil Internal intercostal Abdominal d h c b PART C 1. (experimental results). 2. a. Answers will vary. b. Answers will vary. c. A measurement of residual volume is needed. 3. Answers will vary. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 76 LABORATORY EXERCISE 47 CONTROL OF BREATHING Laboratory Report Answers PART A 1. Medulla oblongata 2. Ventral 3. Dorsal 4. Hydrogen 5. Increases 6. 7. 8. 9. Reduced Increased Internal intercostals Diaphragm PART B 1. (experimental results) 2. a. Hyperventilation lowers blood carbon dioxide levels, and the breathing center is stimulated to a lesser degree. b. Air that is rebreathed has a higher concentration of carbon dioxide, and thus the breathing center is stimulated to a greater degree. c. Breath holding causes the blood carbon dioxide concentration to increase, stimulating the breathing center to a greater degree. d. Exercise causes an increase in the breathing rate. This is due in part to the joint reflex from proprioreceptors being stimulated and in part to an increasing concentration of blood carbon dioxide. 3. a. It is difficult for a person to breathe normally while thinking about it. b. Depth of breath can be measured by breathing into an instrument that measures air volumes. Assess: Critical Thinking Answer A person who has hyperventilated may lose consciousness under water because of changes in the blood carbon dioxide and oxygen levels causing respiratory alkalosis. This is accompanied by arteriole vasoconstriction to the brain, which deprives the brain tissue of oxygen. Hyperventilation has a minimal influence on oxygen carrying capacity on the hemoglobin which is normally nearly saturated (about 97.5%) in lung capillaries. However, hyperventilation can decrease the carbon dioxide enough during breath-holding that depleted oxygen to the brain could cause unconsciousness. This loss of consciousness could occur before the urge to take the next breath takes place. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 77 LABORATORY EXERCISE 48 URINARY ORGANS Figure Labels FIG. 48.1 1. Kidney 2. Ureter 3. 4. Urinary bladder Urethra FIG. 48.2 1. Minor calyx 2. Major calyx 3. Renal pelvis 4. Renal papilla 5. Ureter 6. 7. 8. 9. 10. Renal pyramid Renal column Renal capsule Renal medulla Renal cortex FIG. 48.4 1. Proximal tubule 2. Glomerulus 3. Nephron loop 4. 5. 6. Glomerular capsule Distal tubule Collecting duct Laboratory Report Answers PART A 1. e 2. a 3. h 4. f 5. i 6. 7. 8. 9. c d g b PART B 1. A renal corpuscle is the cluster of capillaries (glomerulus) and the saclike structure (glomerular capsule) that surrounds it; a renal tubule is the coiled tube that leads away from the glomerular capsule and empties into a collecting duct. 2. 4 6 3 5 1 2 7 3. 1 3 2 4 5 PART C (sketches) PART D (sketches) Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 78 LABORATORY EXERCISE 49 URINALYSIS Instructional Suggestion Because most students’ urine will produce negative results for glucose, protein, ketones, bilirubin, and hemoglobin, you may want to provide samples of “artificial urine” (distilled water that contains weak concentrations of some of these substances). By performing the urinalysis test on such samples, students will be able to obtain some positive results. Normal and abnormal simulated urine specimens are suggested as a substitute for collected urine. Laboratory Report Answers PART A 1. (test results) 2. Answers will vary. Assess: Critical Thinking Answer If urine is not refrigerated, substances within it will begin to change as a result of bacterial action, and the composition of the urine will be altered. PART B 1. (sketches) 2. Answers will vary. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 79 LABORATORY EXERCISE 50 MALE REPRODUCTIVE SYSTEM Figure Labels FIG. 50.1 1. Ductus deferens (vas deferens) 2. Urethra 3. Penis 4. Glans penis 5. Prepuce 6. Testis 7. 8. 9. 10. 11. Scrotum Seminal vesicle Prostate gland Bulbourethral gland Epididymis 8. 9. 10. 11. 12. 13. 14. Epididymis Fructose Prostate Bulbourethral Glans penis Emission Ejaculation FIG. 50.2 6 1 4 3 7 5 2 Laboratory Report Answers PART A 1. Spermatic cord 2. Inguinal canal 3. Lobules 4. Epididymis 5. Spermatogenic 6. Meiosis (spermatogenesis) 7. 23 PART B 1. (sketches) 2. a. The scrotum provides a lower body temperature for spermatogenesis in the seminiferous tubules of the testes and storage of sperm cells in the epididymis. b. Spermatogenic cells give rise to sperm cells by meiosis (spermatogenesis). c. Interstitial cells produce and secrete male sex hormones. d. The epididymis stores sperm cells while they mature and propels them into the ductus deferens. e. The corpora cavernosa and corpus spongiosum form three columns of erectile tissue that contain vascular spaces (sinuses) that become engorged with blood during an erection. They also form most of the body of the penis. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 80 LABORATORY EXERCISE 51 FEMALE REPRODUCTIVE SYSTEM Figure Labels FIG. 51.1 1. Suspensory ligament of ovary 2. Uterine tube 3. Ovarian ligament 4. Round ligament of uterus 5. 6. 7. 8. Fimbriae of uterine tube Broad ligament Left ovary Uterus FIG. 51.2 1. Uterine tube 2. Ovary 3. Uterus 4. Clitoris 5. Labium minus 6. 7. 8. 9. 10. Labium majus Vaginal orifice Fimbriae Cervix Vagina 5. 6. 7. Labium majus Labium minus Anus FIG. 51.3 1. Clitoris 2. External urethral orifice (meatus) 3. Vaginal orifice/vestibule 4. Mons pubis FIG. 51.4 5 10 9 4 3 8 1 2 7 6 FIG. 51.5 1. Areola 2. Nipple 3. Lactiferous duct 4. Alveolar glands 5. Adipose tissue Laboratory Report Answers PART A 1. Pelvic 2. Broad 3. Ovarian follicles 4. Oogenesis 5. Follicular 6. First polar body 7. FSH 8. Ovulation 9. Fallopian tubes 10. 11. 12. 13. 14. 15. 16. 17. 18. Infundibulum Cervix Endometrium Smooth muscle Hymen Vulva Mons pubis Clitoris Vestibular glands PART B 1. (sketches) 2. a. A mature antral follicle swells and ruptures under the influence of certain hormones. As this happens, the secondary oocyte and follicular fluid escape from the ovary. b. The cilia that line the uterine tube beat toward the uterus and help draw the secondary oocyte into the infundibulum of the tube and continue to move it toward the uterus. c. The uterine lining thickens and then it becomes glandular and vascular. If fertilization does not occur, the lining disintegrates and sloughs away, creating the menstrual flow. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 81 LABORATORY EXERCISE 52 FERTILIZATION AND EARLY DEVELOPMENT Instructional Suggestions Sea urchin egg and sperm suspensions can be obtained from ripe sea urchins. The “Sea Urchin Embryology Kit” from Carolina Biological Supply Company is one source of these materials. Figure Labels FIG. 52.1 1. Ovulation 2. Zygote FIG. 52.2 1. Yolk sac 2. Mesoderm 3. Endoderm 4. Chorion 3. 4. Morula (late) Blastocyst 5. 6. 7. Amnion Chorionic villi Connecting stalk Laboratory Report Answers PART A 1. Secondary oocyte 2 Acrosome 3. Zygote 4. 5. 6. Mitosis Cleavage Cilia 7. 8. 9. Three Blastocyst Embryo 10. Fetus 4. 5. 6. b i g 7. 8. 9. e c j 10. d PART B (sketches) PART C (sketches) PART D 1. f 2. h 3. a Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 82 LABORATORY EXERCISE 53 GENETICS Laboratory Report Answers PART A 1. (experimental results) 2. (experimental results); Answers will vary. PART B 1. Probability: 10/20 heads; 10/20 tails (experimental results) 2. Probability: 8/24 number below 3 (experimental results) 3. Probability: 8/32 of two heads; 16/32 of a heads and a tails; 8/32 of two tails (experimental results) 4. Probability: 8/32 of two odd numbers; 16/32 of an odd and an even number; 8/32 of two even numbers (experimental results) 5. O = odd numbers E = even numbers O 6. E O OO OE E OE EE a. Yes Deviations from expected probabilities (outcomes) occur because of random variation or chance. The results are closer to expected probabilities as trial numbers increase. b. No Each toss of a coin is an independent event. c. ½ or 50% Each fertilization is a separate independent event. d. 1/16 Each fertilization has the probability of ½ for a girl. Therefore, the chances are ½ · ½ · ½ · ½ = 1/16 PART C 1. a. Rr x rr b and c. 2. 3. R r r Rr rr r Rr rr d. Genotypes: 50% Rr; 50% rr (1:1 genotypic ratio) Phenotypes: 50% tongue rollers; 50% nonrollers (1:1 phenotypic ratio) a. Ff x Ff b and c. F f F FF Ff f Ff ff d. Genotypes: 25% FF; 50% Ff; 25% ff (1:2:1 genotypic ratio) Phenotypes: 75% freckles; 25% no freckles (3:1 phenotypic ratio) a. IBi x IAIA b and c. IB i IA IA IB IA i IA IA IB IA i d. Genotypes: 50% IAIB; 50% IAi (1:1 genotypic ratio) Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 83 Phenotypes: 50% AB; 50% A (1:1 phenotypic ratio) Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 84 4. a. 1Ai x IBi b and c. IA IB IA IB i IA i i IBi ii IAIB; 5. d. Genotypes: 25% 25% IAi, 25% IBi, 25% ii (1:1:1:1 genotypic ratio) Phenotypes: 25% AB; 25% A; 25% B; 25% O (1:1:1:1 phenotypic ratio) a. XHXh x XHY b and c. XH Xh XH X HX H X HX h Y X HY XhY d. Genotypes: 25% XHXH; 25% XHXh; 25% XHY; 25% XhY (1:1:1:1 genotypic ratio) Phenotypes: 50% normal females; 25% normal males; 25% males with hemophilia (2:1:1 phenotypic ratio). Note that all females will have normal blood clotting. Assess: Critical Thinking Answers 1. WwEe x WwEe 2-3. 4. WE We wE we WE WWEE WWEe WwEE WwEe We WWEe WWee WwEe Wwee wE WwEE WwEe wwEE wwEe we WwEe Wwee wwEe wwee Genotypes 1 WWEE; 2 WWEe; 2 WwEE; 4 WwEe; 1 WWee; 2 Wwee; 1 wwEE; 2 wwEe; 1 wwee (1:2:2:4:1:2:1:2:1 genotypic ratio) Phenotypes: 9 widow’s peak, free earlobe; 3 widow’s peak, attached earlobe; 3 straight hairline, free earlobe; 1 straight hairline, attached earlobe (9:3:3:1 phenotypic ratio) Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 85 LABORATORY EXERCISE 54 CAT DISSECTION: MUSCULATURE Instructional Suggestions 1. You may want to provide the students with preserved cats that have been preskinned. Such cats are available from various biological supply companies. The use of preskinned cats will save about an hour of class time and avoid the unpleasantness that some students associate with the skinning procedure. 2. You may want to reduce the amount of time spent in dissecting the cat muscles by having the students dissect only selected areas of the cat. For example, you might have them complete Procedure D (muscles of the thorax), Procedure E (muscles of the abdominal wall), Procedure F (muscles of the shoulder and back), and Procedure H (muscles of the hip and hindlimb), omitting the other sections. Laboratory Report Answers PART A 1. Sternocleidomastoid 2. Masseter 3. Sternohyoid, mylohyoid (also geniohyoid and thyrohyoid) 3. External oblique Internal oblique Transversus abdominis Rectus abdominis 3. 4. 5. Supraspinatus Infraspinatus Infraspinatus Teres major PART D 1. Biceps brachii Brachialis 2. 3. Epitrochlearis Triceps brachii PART E (FIG. 54.25) 1. Pectoralis major 2. Biceps brachii 3. External oblique 4. Rectus abdominis 5. Sartorius 6. Rectus femoris 7. Vastus medialis 8. Tibialis anterior 9. 10. 11. 12. 13. 14. 15. 16. Infraspinatus Teres major Triceps brachii Latissimus dorsi Gluteus medius Gluteus maximus Biceps femoris Gastrocnemius PART B 1. Pectoantebrachialis Xiphihumeralis 2. Pectoralis major Pectoralis minor PART C 1. Clavotrapezius Acromiotrapezius Spinotrapezius 2. Clavodeltoid (clavobrachialis) Acromiodeltoid Spinodeltoid Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 86 LABORATORY EXERCISE 54 FETAL PIG DISSECTION: MUSCULATURE Instructional Suggestions 1. Due to changes from fewer small pig farms to large scale pig production operations, fewer pigs are available. The increased costs can be reduced by dissecting smaller fetal pigs (musculature becomes more difficult), increasing the group size, and reusing dissected pigs with future classes. 2. You may want to reduce the amount of time spent in dissecting the fetal pig muscles by having the students dissect only selected areas of the fetal pig. For example, you might have them complete Procedure E (muscles of the thorax), Procedure F (muscles of the abdominal wall), Procedure G (muscles of the shoulder and back), and Procedure I (muscles of the hip and hindlimb), omitting the other sections. Laboratory Report Answers PART A 1. Sternocleidomastoid 2. Masseter 3. Sternohyoid, mylohyoid 3. External oblique Internal oblique Transversus abdominis Rectus abdominis PART C 1. Clavotrapezius (ventral portion of brachiocephalic) Acromiotrapezius Spinotrapezius 2. 3. Supraspinatus Infraspinatus PART D 1. Biceps brachii Brachialis 2. Triceps brachii 9. 10. 11. 12. 13. 14. 15. 16. Infraspinatus Teres major Triceps brachii Latissimus dorsi Gluteus medius Gluteus maximus Biceps femoris Gastrocnemius PART B 1. Pectoralis major Pectoralis minor 2. Superficial pectoral Posterior deep pectoral Anterior deep pectoral PART E (FIG. 54.12) 1. Deltoid 2. Biceps brachii 3. External oblique 4. Rectus abdominis 5. Sartorius 6. Rectus femoris 7. Vastus medialis 8. Tibialis anterior Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 87 Laboratory Report Answers LABORATORY EXERCISE 55 CAT DISSECTION: CARDIOVASCULAR SYSTEM PART A 1. The parietal pericardium forms a relatively thick, tough sac that encloses the heart. It is attached to the large blood vessels at the base of the heart and to the diaphragm. 2. The walls of the atria are much thinner than those of the ventricles. The wall of the left ventricle is much thicker than that of the right ventricle. 3. Wall thickness is related to the force of its contraction and the amount of pressure it imparts to the blood inside a heart chamber. The left ventricle has the thickest wall, contracts with the greatest force, and creates the greatest amount of blood pressure in the heart chambers. The left ventricle is the pump for the systemic circuit. 4. In the human, the right common carotid artery branches from the brachiocephalic artery, whereas the left common carotid artery comes directly from the aortic arch. In the cat, both common carotid arteries branch from the brachiocephalic artery. 5. In the human, the aorta divides to form the two common iliac arteries, which in turn give rise to external and internal iliac arteries. In the cat, the aorta divides to form the external iliac arteries, and the internal iliac arteries branch from the aorta independently. PART B 1. In the human, the brachiocephalic vein is formed by the union of the internal jugular and the subclavian vein on each side. In the cat, the brachiocephalic vein is formed by the union of the external jugular and the subclavian vein on each side. 2. In the human, the internal jugular vein is somewhat larger than the external jugular vein. In the cat, the external jugular vein is larger. 3. Answers will vary as there are more than twelve correct answers. Most of the veins in the cats are also found in humans. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 88 Laboratory Report Answers LABORATORY EXERCISE 55 FETAL PIG DISSECTION: CARDIOVASCULAR SYSTEM PART A 1. The parietal pericardium forms a relatively thick, tough sac that encloses the heart. It is attached to the large blood vessels at the base of the heart and to the diaphragm. 2. The walls of the atria are much thinner than those of the ventricles. The wall of the left ventricle is much thicker than that of the right ventricle. 3. Wall thickness is related to the force of its contraction and the amount of pressure it imparts to the blood inside a heart chamber. The left ventricle has the thickest wall, contracts with the greatest force, and creates the greatest amount of blood pressure in the heart chambers. The left ventricle is the pump for the systemic circuit. 4. In the human, the right common carotid artery branches from the brachiocephalic artery, whereas the left common carotid artery comes directly from the aortic arch. In the fetal pig, both common carotid arteries branch from the brachiocephalic artery. 5. In the human, the aorta divides to form the two common iliac arteries, which in turn give rise to external and internal iliac arteries. In the fetal pig, the aorta divides to form the external iliac arteries, and the internal iliac arteries branch from the aorta independently. (The umbilical arteries originate from the internal iliac arteries.) PART B 1. In the human, the internal jugular vein is somewhat larger than the external jugular vein. In the fetal pig, the external jugular vein and the internal jugular vein are a similar size. 2. Answers will vary as there are more than twelve correct answers. Most of the veins in pigs are also found in humans. Assess: Critical Thinking Answer Fetal lungs are nonfunctional until birth. The oxygen originates from the mother’s breathing, and this oxygen enters fetal circulation at the placenta. The umbilical vein which is returning blood from the placenta is oxygen-rich until birth. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 89 LABORATORY EXERCISE 56 CAT DISSECTION: DIGESTIVE SYSTEM Laboratory Report Answers PART A 1. The major salivary glands (parotid, submandibular, and sublingual) in the human and the cat occupy similar locations. 2. The adult cat has 6 incisors, 2 canines, 8 premolars, and 2 molars in the upper jaw; the lower jaw teeth are similar, except 4 premolars are present. The adult human has 4 incisors, 2 canines, 4 premolars, and 6 molars in the upper and lower jaw. 3. The cat’s canine teeth are adapted for stabbing and holding prey whereas its rear molars are adapted for cutting meat. 4. The uvula is missing in the cat. 5. The transverse ridges help to hold food. 6. Many of the papillae on the cat’s tongue have spiny projections that help the cat to clean its fur. These are lacking on the human tongue. PART B 1. The peritoneum is the membrane that lines the abdominal cavity and covers the abdominal organs. Double-layered folds in this membrane form the mesentery that supports the abdominal organs. 2. The inner lining of the stomach is folded to form many ridges called gastric folds (rugae). 3. The cat’s liver has five lobes; the human liver has four. 4. The cat’s pancreas is relatively smaller than that of the human and it is double-lobed. One lobe lies along the duodenum, and the other extends behind the stomach toward the spleen. 5. The appendix is missing in the cat. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 90 LABORATORY EXERCISE 56 FETAL PIG DISSECTION: DIGESTIVE SYSTEM Laboratory Report Answers PART A 1. The major salivary glands (parotid, submandibular, and sublingual) in the human and the pig occupy similar locations. 2. The jaw of the baby pig has six incisors, two canines, eight premolars, and zero molars; the jaw of the human child has four incisors, two canines, zero premolars, and four molars. 3. The pig’s canine teeth are adapted for an omnivorous diet as in humans. The pig has larger canine teeth than in the human that aid in grasping food. 4. The uvula is missing in the pig. 5. The transverse ridges help to hold food. 6. Many papillae are near the tip and base of the tongue in the pig. The papillae are sensory and function in food manipulation. PART B 1. The peritoneum is the membrane that lines the abdominal cavity and covers the abdominal organs. Double-layered folds in this membrane form the mesentery that supports the abdominal organs. 2. The inner lining of the stomach is folded to form many ridges called gastric folds (rugae). 3. The pig’s liver has five lobes; the human liver has four. 4. The pig’s large intestine is on the left side and is composed of a predominant spiral colon. The human large intestine has an ascending colon on the right side which is connected to a descending colon on the left side by a transverse colon. 5. The appendix is missing in the pig. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 91 LABORATORY EXERCISE 57 CAT DISSECTION: RESPIRATORY SYSTEM Laboratory Report Answers 1. The auditory tube allows air to pass between the cavity of the middle ear and the outside environment. As a result, air pressure normally remains equal on both sides of the eardrum. 2. The glottis is the opening of the superior (anterior in cats) end of the larynx. The epiglottis is a flap-like structure that shunts food and liquid away from the glottis during swallowing. 3. The tracheal rings are incomplete as they are in the human. 4. The structure of the primary (main) bronchi and the trachea are similar. 5. The cat has three main lobes in each lung (anterior, middle, and posterior). The posterior lobe on the right side has an accessory lobe associated with it, making a total of four lobes on the right lung. The human has three lobes in the right lung and two in the left. 6. The diaphragm is attached to the rim of the thorax and to a central tendon. 7. The heart, esophagus, trachea, and thymus gland are found in the mediastinum. 8. The major structures in the mediastinum of the cat are also found in the human mediastinum. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 92 LABORATORY EXERCISE 57 FETAL PIG DISSECTION: RESPIRATORY SYSTEM Assess: Critical Thinking Answers Fetal lung tissue lacks residual air because the lungs have never inflated which occurs during the first breath. Therefore, fetal lung tissue is more dense than water and will sink. Lung tissue with any residual air in it will float when placed in water. Laboratory Report Answers 1. The auditory tube allows air to pass between the cavity of the middle ear and the outside environment. As a result, air pressure normally remains equal on both sides of the eardrum. 2. The glottis is the opening of the superior (anterior in pigs) end of the larynx. The epiglottis is a flap-like structure that shunts food and liquid away from the glottis during swallowing. 3. The tracheal rings are incomplete as they are in the human. 4. The pig has three lobes in the left lung and four lobes in the right lung. The human has three lobes in the right lung and two in the left. 5. The diaphragm is attached to the rim of the thorax and to a central tendon. 6. The heart, esophagus, trachea, and thymus gland are found in the mediastinum. These are the same major structures found in the human mediastinum. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 93 LABORATORY EXERCISE 58 CAT DISSECTION: URINARY SYSTEM Laboratory Report Answers 1. In the human, the left kidney is usually higher than the right one; in the cat, the right kidney is usually somewhat anterior to the left one. In both cases, the kidneys are retroperitoneal. 2. In the human, the adrenal glands are located on the superior ends of the kidneys; in the cat, the adrenal glands are located anteriorly and medially to the kidneys. 3. In the cat, the kidneys, ureters, and urinary bladder are retroperitoneal. 4. The wall of the urinary bladder is relatively thick, tough, and somewhat elastic. Its inner surface is irregular. 5. In the human kidney, there are about twelve renal pyramids that communicate with the renal pelvis by calyces. Each calyx bears one or more papillae. In the cat kidney, there is a single pyramid and one papilla. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 94 LABORATORY EXERCISE 58 FETAL PIG DISSECTION: URINARY SYSTEM Laboratory Report Answers 1. In the human, the left kidney is usually higher than the right one; in the pig, the kidneys are at similar positions on both sides. In both cases, the kidneys are retroperitoneal. 2. In the human, the adrenal glands are located on the superior ends of the kidneys; in the fetal pig, the adrenal glands are located anteriorly and medially to the kidneys. 3. During fetal development the allantoic bladder eliminates wastes via the allantoic stalk. As a result, the mother eliminates the metabolic wastes for the fetus until birth. 4. The wall of the urinary bladder is relatively thick, tough, and somewhat elastic. Its inner surface is irregular. 5. The termination of the urethra of a female pig is the urogenital sinus a short distance from the urogenital orifice. The human urethra has its own external opening, the external urethral orifice (urinary meatus), just anterior to the vaginal orifice. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 95 LABORATORY EXERCISE 59 CAT DISSECTION: REPRODUCTIVE SYSTEMS Laboratory Report Answers PART A 1. The cat uterine tubes are small, coiled tubes near the ovaries that travel relatively short distances to join the uterine horns. The human uterine tubes are much larger and longer, traveling from the region of the ovaries to the uterine body. 2. The human uterus contains a single chamber in which the offspring develop. The cat uterus is Y-shaped with branches, forming right and left uterine horns. 3. The uterine horns of the cat provide room for many offspring to develop at one time. 4. In the cat, the vagina and urethra open into a common urogenital sinus; in the human each of these organs has a separate opening to the outside. PART B 1. The glans penis of the cat has small spines on its surface, whereas that of the human does not. 2. In the cat, the prostate gland is relatively small and is located some distance from the urinary bladder (it does not surround the urethra). In the human, the prostate gland is relatively larger and surrounds the urethra near the base of the bladder. 3. The seminal vesicles are lacking in the cat. 4. The prepuce of the cat encloses the entire penis for protection. In the human, the prepuce or foreskin is vestigial and only covers the glans penis. A circumcision is commonly performed on humans to remove the prepuce for sanitation and other reasons. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 96 LABORATORY EXERCISE 59 FETAL PIG DISSECTION: REPRODUCTIVE SYSTEMS Laboratory Report Answers PART A 1. The fetal pig uterine tubes are small, coiled tubes near the ovaries that travel relatively short distances to join the uterine horns. The human uterine tubes are much larger and longer, traveling from the region of the ovaries to the uterine body. 2. The human uterus contains a single chamber in which the offspring develop. The fetal pig uterus is Y-shaped with branches, forming right and left uterine horns. 3. The uterine horns of the pig provide room for many offspring to develop at one time. 4. In the fetal pig, the vagina and urethra open into a common urogenital sinus; in the human, each of these organs has a separate opening to the outside. PART B 1. The ovaries and testes are homologous structures that originate near the dorsal abdominal wall posterior to the kidneys. In the male fetus, the testes migrate through the inguinal canal by means of the gubernaculum until they reach the scrotum which provides an environment better for spermatogenesis and sperm storage. Because fetal pigs were not full term (gestation), the testes are located at some point within the inguinal canal. 2. In the fetal pig, the bulbourethral glands are large along the urethra near the proximal end of the penis; in the human, the bulbourethral glands are the smallest of the accessory glands located also along the urethra near the proximal end of the penis. 3. The prepuce of the fetal pig encloses the entire penis for protection. In the human, the prepuce or foreskin is vestigial and only covers the glans penis. A circumcision is commonly performed on humans to remove the prepuce for sanitation and other reasons. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 97 LABORATORY EXERCISE 60 SKELETAL MUSCLE CONTRACTION Laboratory Report Answers PART A 1. h 2. a 3. c 4. i 5. b 6. 7. 8. 9. f g d e PART B 1. Answers will vary. 2. (experimental results) 3. (experimental results) Assess: Critical Thinking Answer Probably not; differences in electrolyte concentrations and various other factors would probably result in slightly different values. PART C 1. (attached myogram) 2. (labels for myogram) 3. Answers will vary. PART D 1. (attached myogram) 2. (labels for myogram) 3. (experimental results) 4. (experimental results) 5. Answers will vary. 6. (experimental results) Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 98 LABORATORY EXERCISE 61 NERVE IMPULSE STIMULATION Laboratory Report Answers PART A 1. Potassium 2. Sodium 3. Potassium 4. Resting potential 5. Depolarized 6. Summation 7. Repolarized 8. 9. 10. 11. 12. 13. 14. Repolarization Refractory period All-or-none Lipid Schwann cells Saltatory Faster PART B 1. (experimental results) 2. (experimental results) 3. (experimental results) 4. Answers will vary. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 99 LABORATORY EXERCISE 62 BLOOD TESTING Warning Because of the possibility of blood-borne infections being transmitted from one student to another during blood-testing procedures, it is suggested that the following tests be performed by the instructor. Mammal blood other than human or contaminant-free human blood is suggested as a substitute for collected blood. Laboratory Report Answers PART A (testing results) PART B 1. Answers will vary. 2. Answers will vary. 3. Answers will vary. Assess: Critical Thinking Answer The hematocrit and hemoglobin tests could indicate anemia if the values are lower than normal values. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 100 LABORATORY EXERCISE 63 FACTORS AFFECTING THE CARDIAC CYCLE Laboratory Report Answers PART A 1. Pump the blood to the lungs and body cells 2. SA node 3. Vagus 4. Acetylcholine 5. Accelerator 6. Norepinephrine (noradrenalin) PART B 1. The atria contract as a unit followed by the contraction of the ventricle (single in frogs). 2. (experimental results) 3. (experimental results) 7. 8. 9. 10. 11. Medulla oblongata Blood pressure (stretch) Sympathetic (accelerator) Increase Potassium (K+) 4. The SA node activity at room temperature is slower than the human resting heart rate. Colder temperatures slow the rate; warmer temperatures increase the rate. PART C 1. The force (amplitude) of the contractions normally 2. increases as calcium ions help initiate muscle contractions. There is danger of prolonged contraction. Normally the rate and force of contractions decrease by altering the usual polarized state of cardiac muscle cells. Excessive potassium ions may result in cardiac arrest. Assess: Critical Thinking Answer This will allow the ionization to occur providing K+ and Ca++ when in a solution of water. Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 101 APPENDIX 1: MATERIALS NEEDED This is a composite list of materials needed for the entire lab manual. The amount or number of each item will depend upon the laboratory size and the number of students working as a group. The laboratory manual lists the materials needed at the beginning of each lab, as some of these materials might not be needed if certain labs or learning activities are not attempted. Items indicated with an (*) are learning activities, alternative materials, or online labs. Prepared Microscope Slides Adipose tissue Adrenal gland Areolar connective tissue Artery cross section Blood smear (Wright’s stain) Bone tissue (compact, ground, cross section) Cardiac muscle tissue Cochlea (section)* Dense connective tissue Dorsal root ganglion (section) Ductus deferens, cross section Elastic cartilage Elastic connective tissue Epididymis, cross section Esophagus Fibrocartilage Heavily pigmented human skin* Human chromosomes from leukocytes in mitosis* Human chromosomes* Human scalp or axilla Human spleen section Human thymus section Hyaline cartilage Kidney section Lamellated (Pacinian) corpuscle* Large intestine Letter e slide Lung tissue (emphysema)* Lung tissue (smoker)* Lung, human (normal) Lymph node section Mammalian eye (sagittal section) Mitosis (whitefish blastula) Nervous tissue (spinal cord smear and cerebellum) Neuroglia cells (astrocytes) Olfactory epithelium* Ovary section with maturing follicles Pancreas (normal) Pancreas of diabetic* Parathyroid gland Pathological blood, such as eosinophilia, leukocytosis, leukopenia, and lymphocytosis* Penis, cross section Peripheral nerve (cross section and longitudinal section) Pituitary gland Pseudostratified (ciliated) columnar epithelium (trachea) Purkinje cells from cerebellum* Reticular connective tissue Salivary gland Sea urchin embryos (early and late cleavage) Semicircular canal (cross section through ampulla)* Simple columnar epithelium (small intestine) Simple cuboidal epithelium (kidney) Simple squamous epithelium (lung) Skeletal muscle tissue (cross section and longitudinal section) Small intestine (jejunum) Smooth muscle tissue Spinal cord cross section with spinal nerve roots Stomach (fundus) Stratified squamous epithelium (esophagus) Tactile (Meissner’s) corpuscle* Taste buds* Tattoo* Testis section Thick skin (plantar or palmar) Three colored threads Thyroid gland Trachea (cross section) Transitional epithelium (urinary bladder) Ureter, cross section Urinary bladder wall Uterine tube, cross section Uterine wall section Uterine wall, early menstrual phase* Uterine wall, early proliferative phase* Uterine wall, secretory phase* Vein cross section Apparatus/Supplies/Equipment Copyright © McGraw-Hill Education. All rights reserved. No reproduction or distribution without the prior written consent of McGraw-Hill Education 93 3” x 5” cards ABO blood-typing kit Anatomical charts of various systems Anti-D serum* Astigmatism chart Audiometer* Beakers (assorted sizes) Bicycle pump* Blunt metal probes Bone shears Bright light Bristle or sharp pencil Bunsen burner Calculator Capillary tubes (nonheparinized)* Centrifuge Centrifuge tubes Electrocardiograph (or other instrument for recording an ECG) Colored pencils Compound light microscopes Cot or table Cotton swabs (sterile; disposable) Coverslips Depression microscope slides Dialysis tubing of 1 5/16-inch diameter or greater Dice Disposable mouthpieces for the spirometer Disposable urine-collecting container Dissecting needle (needle probe) Dissecting pins Dissecting trays Dissection instruments (scalpel, probe, scissors, and forceps) Dropper bottles China marker Clock with second hand or timer Electrode cream (paste) Electronic stimulator (or inductorium)* Filter paper Flasks* Forceps Forceps (fine points) Frog board or heavy cardboard Glass funnel Glass plate Glass rod Glass tubing* Graduated cylinder (10 mL) Hand counter (tally)* Hand magnifier Heavy thread Hemoglobinometer* Hemolysis applicator* Heparinized microhematocrit capillary tube* Hook (small)* Hot plate Ice Slide warming box (Rh blood-typing box or view box)* Ichikawa’s color plates or other colorblindness test Identification tags Large plastic bags Kymograph recording system* Laboratory balance Laboratory scoop Laboratory thermometer Lead selector switch Lens paper Long knife Marking pen (washable) Marking pen (washable) Masking tape Medicine dropper Meterstick Microhematocrit centrifuge* Microhematocrit reader* Microscope slides Myograph and stand* Normal and abnormal simulated urine specimens* Nose clips Oil immersion objective on compound microscope* Ophthalmoscope* Paper bags, small Paper cups (small) Paper towels Pen flashlight Pennies (or other coins) Petri dish pH test paper Physiograph* Physiological recording appartus* Pipet rubber bulbs Pipets (1 mL and 10 mL) Plate electrodes and cables or self-sticking leads Pneumograph* Porcelain test plate PTC paper Radiographs of skeletal structures and joints* Reagent strips (individual or combination) to test for the presence of glucose, protein, ketones, bilirubin, and hemoglobin/occult blood in the urine Ring stand and clamp Rubber bands (short and long) Rubber percussion hammer Rubber stoppers, two-hole* Rubber tubing* Safety equipment (first aid kit, disposable latex and vinyl gloves, safety glasses, laboratory coats, and disinfectant solution) Sealing clay (or Critocaps)* Single-edged razor blade* Slide staining rack and tray* Small fish net Small round stickers Small triangular file* Snellen eye chart Sphygmomanometer Spirometer, handheld (dry portable) Spoon* Stereomicroscopes (dissecting microscopes) Sterile cotton Sterile disposable blood lancets* Stethoscope Swivel chair Syringe for U-100 insulin Tallquist test kit* Test tubes Test-tube clamps Test-tube rack Thistle tube Thread Toothpicks (flat) Copyright © 2016 McGraw-Hill Education. This is proprietary material solely for authorized instructor use. Not authorized for sale or distribution in any manner. This document may not be copied, scanned, duplicated, forwarded, distributed, or posted on a website, in whole or part. 94 Transparent plastic millimeter ruler Tuning fork (128 or 256 cps) Twine Urinometer cylinder Urinometer hydrometer Watch that ticks Wax marker Copyright © 2016 McGraw-Hill Education. This is proprietary material solely for authorized instructor use. Not authorized for sale or distribution in any manner. This document may not be copied, scanned, duplicated, forwarded, distributed, or posted on a website, in whole or part. 95 Models/Skeletons Copyright © 2016 McGraw-Hill Education. This is proprietary material solely for authorized instructor use. Not authorized for sale or distribution in any manner. This document may not be copied, scanned, duplicated, forwarded, distributed, or posted on a website, in whole or part. 94 Animal cell Animal mitosis Articulated human skeleton Cat skeleton* (for cat version) Disarticulated human skeleton Disarticulated human skull (Beauchene) Dissectible ear Dissectible eye Dissectible human brain Dissectible human heart Dissectible human torso model with musculature Fetal skull* Human long bone, sectioned longitudinally Human skull, sagittal section Kidney model Larynx model Lung function model* Preserved Materials Animal lung with trachea* Beef or sheep eyes Cats or fetal pigs (double injection) Human brain Mammalian embryos* Living Specimens/Fresh Material Amoeba culture* Chopped fresh red cabbage Egg albumin Fish (small goldfish, guppy, or other) Fresh animal bones, sectioned longitudinally and transversely Fresh animal joint (knee joint preferred)* Fresh chicken bones (radius and ulna from wings)* Fresh chicken eggs* Fresh round beefsteak* Frog (or goldfish) Chemicals, Reagents, and Biologicals 0.5% amylase solution (must be free of sugar) 0.5% quinine sulfate solution or 0.1% Epsom salt solution 0.5% starch solution 0.9% NaCl (aqueous solution) 1% acetic acid or lemon juice 1% glucose solution 1% HCl solution 1% monosodium glutamate (MSG) solution 1% NaCl solution 1% starch solution 10% glucose solution 10% starch solution 2% Novocain solution (procaine hydrochloride)* 3% NaCl (aqueous solution) 5% NaCl solution 5% sucrose solution 95% ethyl alcohol Acetylcholine, 1:10,000 solution Bacterial amylase powder (store in a freezer) Baking soda Benedict’s solution Biuret reagent Caffeine, 0.2% solution Calcium chloride Calcium hydroxide solution (limewater)* Clear carbonated soft drink Corn syrup (Karo)* Male and female pelves* Model of female reproductive system Model of male reproductive system Models of human embryos Muscular model of the lower limb Muscular model of the upper limb Muscular models of male and female pelves Neuron Skeletal muscle fiber model Skin Spinal cord Synovial joints (shoulder, elbow, hip, and knee) Teeth, sectioned Thoracic organs model Tooth model, sectioned Vertebrae (cervical, thoracic, and lumbar examples) Pig or sheep kidneys Sheep brains Sheep or other mammalian hearts Spinal cord with meninges intact* Paramecium culture* Pieces of apple, carrot, and onion or packages of mixed flavors of LifeSavers Plant materials such as leaves, soft stems, fruits, and vegetables* Potatoes Sea urchin egg and sperm suspensions (these can be obtained by using Sea Urchin Embryology Kits sold by Carolina Biological Supply Company) Uncoagulated animal blood Cornstarch Distilled water Epinephrine, 1:10,000 solution Frog Ringer’s solution Glucose Insulin (regular U-100) (HumulinR in 10 mL vial—store in refrigerator) Iodine Iodine-potassium-iodide (IKI) stain Methylene blue (dilute) Molasses (or Karo dark corn syrup) Potassium chloride Potassium hydroxide Potassium iodide Potassium permanganate crystals Powdered charcoal (or ground black pepper) Sedi-stain Sodium bicarbonate Sodium chloride Stopper bottles of cinnamon, sage, garlic powder, oil of clove, oil of wintergreen, and perfume Sudan IV dye Vaseline* Vegetable oil Vinegar Wright’s stain* Copyright © 2016 McGraw-Hill Education. This is proprietary material solely for authorized instructor use. Not authorized for sale or distribution in any manner. This document may not be copied, scanned, duplicated, forwarded, distributed, or posted on a website, in whole or part. 95 APPENDIX 2: LABORATORY SUPPLIERS This list is not complete, but it does contain well-established names recognized by most anatomy and physiology instructors. Additional suppliers often advertise in scientific journals or have booths at scientific association meetings. Some of these companies also have regional offices. Carolina Biological Supply Company 2700 York Road Burlington, NC 27215 www.carolina.com Connecticut Valley Biological Supply Co. 82 Valley Road; P.O. Box 326 Southampton, MA 01073 www.ctvalleybio.com Cynmar Corporation 21709 Route 4 North P.O. Box 530 Carlinville, IL 62626 www.cynmar.com Fisher Scientific U.S. Headquarters 2000 Park Lane Pittsburgh, PA 15275 www.fisheredu.com Flinn Scientific P.O. Box 219 Batavia, IL 60510 www.flinnsci.com Nasco, Inc. 901 Janesville Ave. Fort Atkinson, WI 53538 www.enasco.com Nebraska Scientific 3823 Leavenworth St. Omaha, NE 68105 www.nebraskascientific.com Phipps and Bird/Intelitool P.O. Box 7475 Richmond, VA 23221 www.phippsbird.com Sargent-Welch Scientific Company P.O. Box 4130 Buffalo, NY 14217 www.sargentwelch.com The Scope Schoppe P.O. Box 583 DeKalb, IL 60115 www.scopeshoppe.com Ward’s Natural Science 5100 West Henrietta Road P.O. Box 92912 Copyright © 2016 McGraw-Hill Education. This is proprietary material solely for authorized instructor use. Not authorized for sale or distribution in any manner. This document may not be copied, scanned, duplicated, forwarded, distributed, or posted on a website, in whole or part. 96 Rochester, NY 14692 www.wardsci.com Copyright © 2016 McGraw-Hill Education. This is proprietary material solely for authorized instructor use. Not authorized for sale or distribution in any manner. This document may not be copied, scanned, duplicated, forwarded, distributed, or posted on a website, in whole or part. 97 APPENDIX 3 Student Safety Rules Agreement I have read all of the “Laboratory Safety Guidelines” in Appendix 1 of the laboratory manual. I agree to follow these guidelines, and any additional safety guidelines included in the individual labs, to insure my own personal safety as well as the safety of all persons in the laboratory. I will inform my instructor of any safety violations and any health conditions changes that might impact the safety of anyone in the laboratory. I have read, understood, and agree to the safety rules. Signature Date Copyright © 2016 McGraw-Hill Education. This is proprietary material solely for authorized instructor use. Not authorized for sale or distribution in any manner. This document may not be copied, scanned, duplicated, forwarded, distributed, or posted on a website, in whole or part. 98 APPENDIX 4 Student Informed Consent Form My participation in a particular lab that has any safety or privacy concerns is voluntary; I may choose not to participate in that specific lab, and be required to do an alternative project. The potential risks have been included in the laboratory manual and/or explained to me by my instructor. My individual personal data will be confidential although a summary of class results may be included in a review of the laboratory exercise. I have no known health condition that would prohibit me from participating in this laboratory exercise. Signature Date Copyright © 2016 McGraw-Hill Education. This is proprietary material solely for authorized instructor use. Not authorized for sale or distribution in any manner. This document may not be copied, scanned, duplicated, forwarded, distributed, or posted on a website, in whole or part.