CITRUS SAP AND JACKFRUIT (Artocarpus heterophyllus) SAP AS SHOE GLUE A Science Investigatory Project presented to the Faculty of Corcuera National High School- Junior High School Department In Partial Fulfilment of the Requirements for Special Science Curriculum in Research IV by FLORAMEL JOY F. FALCULAN JENNY ROSE M. FRONDA KENDRICK F. MOTIN ROWENA F. FAN 2020 CERTIFICATION This Science Investigatory Project entitled “JACKFRUIT (Artocarpus heterophyllus) SAP AND SINTURIS (Citrus noblis) SAP AS SHOE GLUE” prepared and submitted by FLORAMEL JOY F. FALCULAN, JENNY ROSE M. FRONDA, KENDRICK F. MOTIN, and ROWENA F. FAN in partial fulfilment of the requirements for Special Science Curriculum in Research IV has been examined and recommended for ORAL EXAMINATION. SHEILA MARIE L. MORES Adviser ______________________________________________________________________ APPROVAL SHEET Approved by the Oral Defense Panel on ___________________ with a rating of __________________. HERNANNIE F. FABIALA, MT I Chairman JASPER KATE F. FADRILAN Member JOB F. FACTOR Member Accepted in partial fulfilment of the requirements for Special Science Curriculum in Research IV. HERNANNIE F. FABIALA, MT I Science Coordinator ABSTRACT Title : JACKFRUIT (Artocarpus heterophyllus) SAP AND SINTURIS (Citrus noblis) SAP AS SHOE GLUE Researchers: FLORAMEL JOY F. FALCULAN JENNY ROSE M. FRONDA KENDRICK F. MOTIN ROWENA F. FAN Curriculum: Special Science Class (SSC) Curriculum Year: 2020 Adviser: SHEILA MARIE L. MORES There are many people who use shoes and they are aware if their shoes open ups. When this case happens, they buy something to put on it which is called shoe glue in order for it to reuse than to throw it away. The researchers decided to conduct a research study that would help people who needs like the government employees, teachers and students which is the jackfruit and sinturis as shoe glue. This would be a great help for the people who benefited from it because it was well-tested and could be bought in an affordable price. The researchers used the experimental design in conducting the study. In this design, it is also a way to carefully plan experiments in advance so that the results are both objective and valid. This design is appropriate in this study because the researchers conducted an experiment to test the effectiveness of Jackfruit sap as shoe glue. In making this product, the researchers follow the procedures; collecting of materials, measuring, mixing, boiling, pouring recording, and concluding. Spearman rho is used to test the degree of relationship between the bond strength and the drying duration to determine whether the bond strength and drying duration depend on each other. The researchers used the experimental design to test the effectiveness of citrus and jackfruit trees as shoe glue. An experiment involves making a change in avalue of one variablecalled the independent variable and observing the effect of that change on another variablecalled the dependent variable. The researchers found out that jackfruit and citrus trees' sap can be good in making a shoe glue. The researchers therefore concluded its effectiveness is significant to people who have damaged shoes like the students, teachers and other local entrepreneurs because it is good in repairing damaged shoes. On the experiments and results, the researchers found out that the quality attributes of the product are possible to be a substitute as shoe glue. It was concluded that the hypothesis was rejected. Therefore, the researchers concluded that Jackfruit and citrus trees' sap can be used to produce a good quality shoe glue. ACKNOWLEDGEMENTS This accomplished study would not be possible without the people who extended their utmost love and support in sharing their knowledge and time to the researchers. The researchers would like to express their sincerest appreciation and warm gratitude to the following individuals behind the completion of this study: Sheila Marie L. Mores, their research teacher and adviser, for her guidance that provides the researchers with inspiration and motivation to finish this study and most especially for the advices that she had given to the researchers while the study was being conducted. Their families, for their moral and financial support while the study was being conducted. Their friends and classmates, who had always been supportive and understanding from the very start up to the completion of this study. Their informants, who gave their time for the researchers' interview and gave a big contribution to their study despite of their busy schedules and for the helpful answers they have given during the interview. And most of all, the researchers would like to thank Heavenly Father, for giving them the knowledge, the courage, and the strength that the researchers need to accomplish this research study. The Researchers DEDICATION The researchers would like to dedicate this research study to the people who helped and encouraged them to pursue this study. To the family of the researchers who inspired them in finishing their study. To the school where the researchers came from. To the people that need this product. The Researchers TABLE OF CONTENTS Page PRELIMINARIES i-vii CHAPTER 1 The Problem and Its Background Introduction 1 Theoretical/Conceptual Framework 2 Statement of the Problem 2 Hypotheses 3 Scope and Limitation of the Study 3 Significance of the Study 3 Definition of Terms 4 2 Review of Related Literature and Studies Related Literatures 6 Related Studies 8 3 Methodology Research Design 10 Materials 10 Equipment/Apparatus and Utensils 11 Procedures 11 Sampling Procedure 13 Respondents of the Study 14 Research Instrument Data Gathering Procedures Statistical Treatment of Data 4 Results and Discussion 14 14 15 16 5 Summary, Findings, Conclusions, and Recommendations Findings 21 Conclusions 21 Recommendations 22 REFERENCES APPENDICES DOCUMENTATION QUESTIONNAIRE LETTERS CERTIFICATION CHAPTER 1 The Problem and Its Background This chapter presents the introduction, conceptual framework, statement of the problem, hypothesis, scope and limitations of the study, significance of the study, and definition of terms. Introduction Glue sticks, super glue, liquid glue and glue spray are common materials found in each classroom. Strikingly, the creation of glue dates to around 200,000 BC, when it was mixed with paint to make cave drawings last longer. Derived from plant and animal materials, glue has been a staple of humankind for millennia. Being only one member of a large class of adhesives, glue works by binding two materials together. It does this through producing a small surface chemical reaction and bond between the materials. Today, the development of the glue industry is moving forward rapidly, and the creation of stronger and more flexible glues are continuously being invented. Jackfruit (Artocarpus heterophyllus) is an indigenous fruit tree that is widely distributed throughout many tropical countries, including Philippines both cultivated and wild. This plant is locally available also in Simara. It contains a sap which has a component that is identical to the white wood glue called latex. With this, the researchers were encouraged to use this as an additive in making a glue because sap is extremely sticky and therefore also utilized as an effective adhesive. On the importance of glue, the function and value of this sticky stuff plays a very important role in the school. The glue sticks things together or something is stuck to other things especially in bonding thin materials. Through this, the object likely to stay together and wouldn’t fall off and get lost. To lessen the duration time of the glue to getting dry, the researchers used alcohol. Alcohol consists mainly of ethanol or isopropanol. Ethanol and isopropanol boil at a lower temperature than water, which generally means that they evaporate quicker than water. The boiling temperature is largely determined by attractive interactions between the liquid molecules. Although commercial shoe glues can be bought at any local market, these products are expensive and release harmful gases to atmosphere that contribute to global warming. According to Oxford Dictionary global warming is a gradual increase in the overall temperature of the earth's atmosphere generally attributedDependent to the greenhouse Independent variable variableeffect caused Jackfruit (Artocarpus by increased levels of carbon dioxide, chlorofluorocarbons, and other pollutants. Effectiveness Shoe Glue heterophyllus) Sap and Citrus Sap Conceptual Framework Figure 1.1. Paradigm of the Independent and Dependent Variables on Jackfruit (Artocarpus heterophyllus) Sap and Sinturis (Citrus noblis) Sap as Shoe Glue Statement of the Problem This research was conducted to produce shoe glue out of jackfruit (Artocarpus heterophyllus) sap and sinturis (Citrus noblis) sap. Specifically, this study was sought to answer the following questions: 1. What type of shoes can the product bind? 2. How fast does the product dry? 3. How strong can the product bind? 4. Does the product’s bond strength depend on its drying duration Hypotheses Base on the foregoing research questions identified, the researchers formulated the following null hypotheses: 1. The product cannot bind materials such as leather, cloth, rubber, and others. 2. The glue does not dry for approximately a minute. 3. The bond strength is not the same for all types of shoes tested. 4. The bond strength does not depends on the product’s drying duration. Scope and Limitation of the Study This study was conducted to prove the efficacy of jackfriut sap (Artocarpus heterophyllus) and sinturis (Citrus noblis) sap as an alternative shoe glue used to repair broken shoes. This study was conducted at Corcuera National High School Science Laboratory from July 2019 to March 2020. The study is limited to the material the product would bind, the product’s strength to bind and how fast the product dries compared to commercial shoe glue. Other parts of the plant involved would not be included in this research. Additionally, the product of the study would not be tested by a laboratory. Significance of the Study The results of the study is beneficial to the following: Researchers The results of the study provide information to other researchers and can be used as their reference for future studies. Students The product of this study can be used by many individuals especially those students that doesn't have enough money to buy expensive shoe glue to fix their shoes. School This study will improve the school in the development of research education. This study will foster new ways of enhancing knowledge, skills and attitude, thus preparing globally-competitive researchers in the future. Definition of Terms The following terms are defined operationally and lexically to provide a common frame of reference: Adhesive it also known as glue, cement, mucilage, or paste, is any non metallic substance applied to one or both surfaces of two separate items that binds them together and resists their separation. Citrus It is a tree of a genus that includes citron, lemon, lime, orange, and grapefruit. Ethanol Ethanol fuel is ethyl alcohol, the same type of alcohol found in alcoholic beverages, used as fuel. Latex It contains a sap which has a component that is identical to the white wood glue Global Warming It the most noticeable changes because of this increase in temperature is the melting of ice caps all around the world. Sap It is a fluid transported in xylem cells or phloem sieve tube elements of a plant. CHAPTER 2 Review of Related Literature and Studies Related Literatures Jackfruit (Artocarpus heterophyllus Lam.) trees belong to the family Moraceae. They grow abundantly in India, Bangladesh, and in many parts of Southeast Asia (Rahaman and others 1999). It is one the most significant evergreen trees in tropical areas and widely grown in Asia including India. It is a medium-size tree typically reaching 28 to 80 ft in height that is easily accessible for its fruit. The fruit is borne on side branches and main branches of the tree. Average weight of a fruit is 3.5 to 10 kg and sometimes a fruit may reach up to 25 kg. The search for lesser known and underutilized crops, many of which are potentially valuable as human and animal food has included jackfruits which have been the focus of research in recent years. Phytochemistry Heterophyllus contains various chemical constituents as several flavone colorings, morin, dihydromorin, cynomacurin, artocarpin, isoartocarpin, cyloartocarpin, artocarpesin, oxydihydroartocarpesin, artocarpetin, norartocarpetin, cycloartinone, and artocarpanone (Rama Rao and others 1973). The heartwood of jackfruit on analysis yields moisture (6.7%), glucosides (38.0%), lipids (0.7%), protein (1.7%), and cellulose (59.0 %) (Perkin and Cope 1895). The jackfruit also contains free sugar (sucrose), fatty acids, ellagic acid, and amino acids like arginine, cystine, histidine, leucine, lysine, metheonine, theonine, tryptophan, and others. (Pavanasasivam and Sultanbawa 1973). Bark from the main trunk contains betullic acid and two new flavone pigments including cycloheterophyllin (C30H30O7) (Chawdhary and Raman 1997). Heterophylol, a phenolic compound with a novel skeleton, was obtained from A. heterophyllus (Chun-Nan and Chai-Ming 1993). The leaves and stem have shown the presence of sapogenins, cycloartenone, cycloartenol, β-sitosterol (Nath and Chaturvedi 1989), and tannins, and they have shown estrogenic activity. The root contains β-sitosterol, ursolic acid, betulinic acid, and cycloartenone (Dayal and Seshadri 1974). Jackfruit (Artocarpus heterophyllus) has also been utilize for industrial purposes. Its branches and trunk are burned for fuel wood. In the province of Cebu, Philippines, the wood is highly prized for making guitars, ukuleles, and other musical instruments (Everything Cebu, 2010). The heated latex can be used as glue for mending chinaware and pottery and as caulking for boats and buckets. The latex contains resins that may have use in varnishes. The latex also has bacteriolytic value comparable to that of papaya latex (Gamboa, 2013). Additionally, the sticky latex is used for trapping birds (birdlime) and for insect traps. The bark contains 3.3 % tannin (Prakash et al., 2009). Tannin is a dye, which is used to give the famous orange-red color to the robes of Buddhist priests Distribution and Diversity of citrus The genus Citrus is one of the 33 genera in the sub-family Aurantioideae of the family Rutaceae. The taxonomy and geographic origin of Aurantioideae have been reviewed by Swingle and Reece (1967). About Citrus Sap Citrus Sap is a sativa-dominant hybrid created as a joint venture between Crockett Family Farms, creators of Clementine, and the prolific DNA Genetics, who have given us flavorful strains like Lemon Skunk and Chocolope. Citrus Sap is a cross between powerful indica Gorilla Glue #4 and flavorful sativa Tangie. Despite its mostly sativa genetics, this strain tends to offer powerfully sedative effects. Besides leaving behind a lingering cloud of sweet, citrus funk, Citrus Sap is liable to leave you couch locked and daydreaming. Its THC content has been measured at between 17% and 24%. Flowers of Citrus Sap tend to have a conical shape, tapering down from a broad base into a pointed tip. They range in size from small to medium and cling together in the dense, solid structure typically associated with indicas. The leaves are a pale shade of spring green and are threaded through with dark, rust-colored hairs (which are actually pistils, structures meant to catch pollen from fertilizing male plants). True to the “sap” in their name, flowers of Citrus Sap are covered in silvery resinous trichomes, giving them a frosty appearance. Like parent strain Tangie, Citrus Sap has a predominantly citrus smell, redolent of oranges and tangerines. Underlying this sharp aroma is a sweetness that verges on sour, with a slightly rank, fermented edge. On closer inspection, these buds also carry the woodsy odor of pine. Meanwhile, grinding up or breaking open the flowers yields some slightly skunky notes. When burnt in a joint or a pipe, Tangie gives off a thick and lung-expanding smoke that may induce coughing or cause eyes to water. On the exhale, this bud’s flavors meld, as the smoke leaves behind the tastes of citrus-inflected pine sap. Citrus Sap offers a slow, creeping high that may only set in several minutes after smokers have savored its unique, rich flavor. Initial effects take the form of full-body relaxation, with tingling, soothing waves spreading from the neck down through the core and limbs. Citrus Sap also comes with a trippy sensation, as it distorts perception of external stimuli like sights and sounds. This psychedelic edge makes it a great accompaniment to spacey movies or music, as well as for lifted conversations with like-minded friends. Indeed, the bud’s effects can be very social, facilitating giddiness and camaraderie. Although Citrus Sap provides some mental stimulation, any cerebral activity takes the form of rambling, circuitous daydreaming rather than an intense, analytical mindset; smokers should not plan on accomplishing a mountain of work after enjoying this aromatic strain. As the high wears on or as dosage is increased, couch lock becomes more and more likely. Enjoy this strain as a way to unwind after a long day or week of work. Because of its mostly sleepy vibes, Citrus Sap is best enjoyed during the evening or late at night. A rare sleepy sativa, Citrus Sap makes it mark with potent effects and a memorable scent. This bud is a good resource for kicking back alone or with friends. Its high resin content can also be valuable in the production of hash and other THC concentrates. Related Studies Jackfruit: Artocarpus heterophyllus Lam. synonyms of the species of jackfruit (botanical or scientific name: Artocarpus heterophyllus Lam.) are: A. philippinensis Lam., A. maxima Blanco, Soccus arboreus major Rumph., Polyphema jaca Lour., A. brasiliensis Gomez (Soepadmo, 1992). Nevertheless, since various synonyms given to this species there is some confusion in relation to taxonomic nomenclature. One of the synonymes which is often used is A. integrifolius Auct. Nevertheless, it actually belongs to A. integer (Thunb.) Merr. General morphology It is described as an evergreen medium-sized to large tree with 10-20 m tall, (sometimes even reaching 30 m), with a dense crown and a long taproot. The crown is conical when the trees are young or grown under shaded conditions and reaches a diameter of 3.5-6.7 m at five years; becoming rounded and somewhat irregular when older. The bark is described as somehow scaly and greyish brown or dark grey.Branches spread from low down the trunk while inserted at angles ranging from 30- 90º. Twigs are cylindrical and mainly smooth but sometimes with short, white hairs, which become smooth later. Renuka Prasad and Virupaksha [114] have purifed a protease named ‘artocarpin’, from jackfruit latex which has showed proteolytic activity against casein. Te jackfruit latex also can be used as an adhesive. Jack tree latex has the same composition as the fruit gum. Besides proteins and mineral substances, the two contain a high percentage of waxy matter. The less soluble portion (small amount) of the wax yields on saponification a non-steroid substance melting at 96-7° and having probable composition of C30H58O2. The more soluble major fraction yields artostenone on saponification. Experiments indicate definitely that this ketone is not occurring free and that it is formed as a result of the saponification. Though artostenone does not have the capacity of forming enolesters, α-artostenone yields an enol-benzoate readily. Further α-artostenone and its enol-ester undergo conversion into artostenone under the conditions of saponification. Hence the conclusion is drawn that in the plant it is αartostenone that occurs in the form of enol-wax-esters. Citrus sap More and more British homeowners are bringing the Mediterranean to their gardens by investing in a fruit tree or two. These colourful additions can brighten up a courtyard, orchard or lawn and keep you self-sufficient in whichever variety of fruit takes your fancy. Although they’re not particularly difficult to maintain, fruit trees can leave their owners literally stumped when they encounter a problem. One of the most frequently asked questions about lemon trees in particular is why they have sticky leaves. We will attempt to address that right here but if you’re unsure of what to do, it’s always worth getting a professional to come out and take a proper look. leaves of any tree are usually evidence of an aphid infestation or occasionally an infestation of a tree above the lemon tree. Aphids feed on the sap of phloem vessels – this is the living tissue that carries organic nutrients to the plant for healthy growth. These insects pierce the leaf using their mouth and suction up the sap into their food canal. Aphids suction up more sap than they can physically digest which means the excess drops onto leaves below. As the sap dries, it leaves a sticky, sugary substance known as honeydew. Occasionally the sap residue will also be flecked with black. This indicates the presence of a fungus called sooty mould. While this might appear alarming, the fungus relies on the sap itself to feed and not the leaves, therefore causing no further damage. Colonies of ants often eat the honeydew which is rich in energy. This provides the lemon tree with additional protection from predators and creates an ecological balance Where infestations are unmanageable, it is advisable to prune your lemon tree to remove some of the insect colonies. Another useful tip is to spray the lemon tree with water from a hose which will help to wash and disperse some of the population and prevent them from finding their way back. It is possible to buy natural oils such as Neem oil which infects and kills aphids and won’t harm your tree. You could also try and introduce other predators organically to control your aphid populations. Ladybirds and wasps are particularly partial to aphids. The good news is lemon trees are one of the toughest citrus trees and will withstand many problems, including aphid infestations. CHAPTER 3 Methodology Research Design The researchers used the experimental design to test the effectiveness of citrus and jackfruit trees sap as shoe glue. According to Sugiyono (2006:80), experimental research is a research which has the purpose to find the cause-effect relationship among variables in a controlled condition. The essential feature of experimental research is that investigators deliberately control and manipulate the conditions which determine the events, in which they are interested, introduce an intervention and measure the difference that it makes. An experiment involves making a change in the value of one variable – called the independent variable and observing the effect of that change on another variable – called the dependent variable (Louis, 2007:291). Materials The materials used in the study were jackfruit sap, 20ml; citrus tree sap , 20ml; and alcohol, 5ml . Table 3.1 shows the materials and ingredients used in making of sinturis (Citrus noblis) sap and jackfruit (Artocarpus heterophllus) sap as shoe glue. Quantity Materials and Ingredients Jackfruit sap 20 Citrus Trees sap 20 Alcohol 5 Table 3.1. Materials and Ingredients in Making of Sinturis (Citrus noblis) Sap and Jackfruit (Artocarpus heterophyllus) Sap as Shoe Glue Equipment and Utensils The equipment and utensils used in the preparation of this study were pot, spoon and container. Table 3.2 shows the equipment and utensils used in making of sinturis (Citrus noblis) sap and jackfruit (Artocarpus heterophllus) sap as shoe glue. Equipment and Utensils Quantity Pot 1 unit Spoon 1 unit Container 1 unit Table 3.2. Equipment and Utensils in Making of of Sinturis (Citrus noblis) Sap and Jackfruit (Artocarpus heterophyllus) Sap as Shoe Glue Procedures First, the researchers gathered the ingredients and prepared the materials needed. Next, they heated the pot. Then, they measured 20ml of jackfruit sap and sinturis sap. When the two saps are already measured, they put it on the pot and mixed it thoroughly until it totally melted. Lastly, put it on a container and test it to damaged shoes. Figure 3.1. Process Flow Sheet in Making of Sinturis (Citrus noblis) Sap and Jackfruit (Artocarpus heterophyllus) Sap as Shoe Glue Testing the Product The shoe glue produced from citrus sap and jackfruit (Artocarpus heterophllus) sap were applied to different types of surfaces of shoes such as leather, rubber, and fabric. The efficacy of the product was tested by shoe glue’s bond duration and bond strength. Measuring of the Bond Strength It was done by using different levels of wind strength using the anemometer: level one is assigned to be 9 m/s; level two is 10 m/s, and level three is 11 m/s. it was tested by applying the product, increasing in amount every trial (amount is measured by how many wipes when applied) onto the surface of shoes and left to dry then exposed to different wind strengths using a fan (Fulgencio et al., 2014). Measuring for the Drying Duration It was done using a timer. Drying duration is the time it took for the different types of shoes to dry. Relationship Between Bond Strength and Drying It was proven by using Spearman Rank-order Correlation to determine the relationship of the bond strength and the drying duration of the ranked surfaces of shoes based on both bond strength and drying duration. Statistical Treatment of Data The statistical treatment used in analyzing and interpreting the data is the Spearman Rank-order Correlation. The formula is as follows: where: rs = Spearman rho ΣD2 = Sum of the squared differences between ranks N = Number of cases CHAPTER 4 Results and Discussions Table 4.1. The observed time it took for the different types of shoes to dry and their bond strength in accelerating the wind strength Surfaces of Shoes Drying Duration (minute) Trial 1 Trial 2 Bond Strength Level (m/s) Trial 3 Trial 1 Trial 2 Trial 3 Leather 18.3 19.4 20.0 9 10 11 Rubber 17.60 18.9 19.9 9 10 11 Heel shoes 18.50 19.7 21.6 8 9 10 The following data are ranked depends upon on the amount of glue that was put on the shoes above and are then evaluated. The table show the time it took for the different types of shoes surfaces to dry and their bond strength at different wind speed. As you can see in the table above , in terms of drying duration , shoes that have a large amount of glue that was put such us leather shoes and rubber shoes , tend to dry faster than the shoes that have a small amount of glue that was put such as heel shoes. Larger amount of glue also dry for a minimum of eight minutes while smaller amount of glue dry for ten minutes and above. It is also observed that shoes with smoother textures such as leather and rubber shoes dry approaximately one minutes slower tha that regular ones. Moreover , in terms of the bond strength level of each shoes surface , shoes with smoother textures easily get separated when in strong wind. In trial one , it is observed that shoes such as leather and fabric withstand strong wind since the leather shoes initially reached the second wind strength level ( equal to 10m/s ) and the rubber for reaching the strongest wind strength level ( equal to 11m/s ) initially then consistently when bonded together. In trial two , it is observed that shoes with smoother textures still easily get separated in strong wind because the iniatial tests showed the least amount of efficacy on both leather and fabric. In trial three , the rubber and fabric reached the strongest level of wind strength while the smooth surfaced shoes increased the bond strength gradually to level two. The statistical treatment used was Spearman Rank Order Correlation to test the relationship between the bond strength and the drying duration to determine whether the bond strength and the drying duration depend on each other . The result showed that p = 0.876 ,thus , pertaining that there is a positive high relationship between the product's drying duration and it's bond strength . This means that the bond strength highly depends on the product's drying duration. CHAPTER 5 Findings 1. Jackfruit and citrus trees' sap can be good in making as shoe glue. 2. It has been found out that the shoe glue produced was cheaper and easy to prepare than other shoe glue. Conclusions 1. Its effectiveness is significant to people who have damaged shoes like the students, teachers and othet local entrepreneurs because it is good in repairing damaged shoes. 2. On the experiments and results, the researchers found out that the quality attributes of the product are possible to be a substitute as shoe glue. 3. The hypothesis was rejected. Therefore, the researchers concluded that Jackfruit and citrus trees' sap can be used to produce a good quality shoe glue. Recommendations 1. The researchers highly recommend to use the produced product in repairing damaged shoes. 2. The researchers also recommend to have a further study if there is any enhancements and improvements that can be seen out of this product and making comparison/comparative experiments with comercially available shoe glues. BIBLIOGRAPHY Online Sources https://www.leafly.com/strains/citrus-sap https://treesurgeryshenfield.co.uk/blog/why-are-the-leaves-on-my-lemon-tree-sticky http://shodhganga.inflibnet.ac.in/jspui/bitstream/10603/169749/8/08_chapter%202 https://www.science.gov/topicpages/j/jackfruit+artocarpus+heterophyllus https://books.google.com.ph/books?id=eh51jh_0008C&pg=PA666&lpg=PA666&dq=citru s+sap+literature+review&source=bl&ots=SfJKX0-bg&sig=ACfU3U39pg9spgI0rtlXvubvUIeNUxH8VQ&hl=en&sa=X&ved=2ahUKEwifhtq CkYLoAhXpFqYKHf4aCVA4ChDoATAAegQIBBAB#v=onepage&q=citrus%20sap%20 literature%20review&f=false https://onlinelibrary.wil ey.com/doi/abs/10.1002 /arch.21394 https://repository.up.ac. za/bitstream/handle/226 3/60855/Sam_Calibrati on_2016.pdf?sequence= 1&isAllowed=y APPENDICES