LO 01.2.01 . '<>llttC the gene that controls the · · t 1 ·1vc been nbl c t0 I s . insert the gene into another type of Using special enzymes, sctcntJs s " . fp . A fl·om one type of bnctcnn IIIH1 , productiOn o rotem t (l . A These b·H.:tcria then produce Protein A. bacteria that does not nonnally produce rotclll · · • What is the name of this technique? A- Cloning B- Meiosis C- Genetic modification c LO 81.2.01 GM crops are different from anything that has existed before because: They are produced by techniques of cross-breeding They are capable of drastically improving agricultural yields They involve .transplanting genes between different organisms LO 81.2.01 Which of the following is not an advantage of producing genetically modified food? A- Producing greater yield within an shorter period of time. B- Producing cereals which contain a variety of nutrients. C- Increasing the resistance to pests and thus reducing the usc of pesticides D- Upsetting the equilibrium in the ecosystem . LO 81.2.01 Large quantities ofuseful products can be produced through genetic engineering involving: A- bacteria containing recombinant plasmids B- yeast carrying foreign genes ;· ~ · ;. C- transgenic plants D- all ofthe above ... BT com is a type of genetic modified crops that is a type of com modified with Bt bacteria, the reason behind this modification A- To increase nutrition in com for developing countries B- To decrease the wastes comes from the com fields C- To get rid of European com borer which take out 5% of the field each year D- To increase resistance to pests BIOLOGY - Grade 11 Page 2 of 16 Scanned by CamScanner 7 LO 81.2.01 Which of the followii1g is not an advantage of producing genetically modified food'! AProducing greater yield within a short period of time. n- Producing cereals which contain a variety of nutrients. Increasing the resistance to pests and thus reducing the use of Cpesticides U · the LO 81.2.02 NliTRJENT AGAR PLATES In a transfommtion experiment, a sample Ampicillin No Ampicillin of E. coli bacteria was mixed with a plasmid containing the gene for resistance to the antibiotic ampicillin (ampr). Plasmid was not added to -;·Wild-lype · E. coli a second sample. Samples were plated on nutrient agar plates, some of which were II supplemented with the antibiotic ampicillin. The results of E. coli growth are summarized E. coli and below. The shaded ar~a represents anrpr plasmld . extensive growth of bacteria; dots represent IV individual colonies of bacteria. u 0 . Use this information to Answer questions 8 and 9: Plates that have only ampicillin-resistant bacteria growing include which of the following? AB- CD- I only Ill only IV only I and I LO 81.2.02 I. Plates I and III were included in the experimental design in order to A- Demonstrate that the E. coli cultures were viable B- Demonstrate that the plasmid can lose its ampr gene C- Demonstrate that the plasmid is needed for E. coli growth are the E. coli for transfonnation LO 81.2.02 2. How can you check to see if the GFP gene was moved into the bacteria Aa. Count how many bacteria you have on your plate Bb. See if the bacteria changed shape Cc. Observe the bacteria under the microscope. Dd. Look at the bacteria under UV light. BIOLOGY • Grade 11 Page 3 of 16 Scanned by CamScanner · that produce human ·msu1·m, scten · f lSts insert the human gene of interest To create bactena into the bacterial: A- Circular chromosome B- Plasmid C- Cell wall DLO 81.2.02 Use the following information to answer the question below. A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes, which make it resistant to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth containing no antibiotics. The bacteria containing the engineered plasmid would grow in --AAmpicillin and tetracycline broth only. B- Nutrient broth, the ampicillin broth, and the tetracycline broth. C- Nutrient broth and the tetracycline broth only. icillin broth and the nutrient broth. Asexual reproduction produces offspring that each contain AGenetic information from one parent B- Genetic information from two parents C- Less genetic information than either parent A un · ue combination of · information LO BI.2.02 Homologus chromosomes include AOne smaller and one bigger chromosome. B- One chromosome from each parent. C- One complete and one incomplete chromosome. D- None of the above. Which of the following is NOT true concerning mitosis? AB- CD- Animal cells have centrioles while plant cells do not. Both plant and animal cells undergo cytokinesis. Mitosis allows growth and increase in size in both plants and animals. Animal cells form a cell plate during c~okinesis while plant cells do not. . BIOLOGY -Grade 11 ,.. Page 4 of 16 Scanned by CamScanner LO 81.2.02 The term thnt refers to division of the cytoplasm is: ACytokinesis UAnaphase CApoptosis kinesis Generally, complex organisms do require more genes to control their synthesis and organization than do primitive orgat:lisms. However, the numbers of chromosomes vary from ants with 2, molds with··s(f4, humans with 46, potatoes with 100 and the crayfish with 200, Given this, then A- There must be no relationship between amount of genetic information and complexity of the organism. B- The number of genes per chromosome may vary among organisms, preventing a simple relationship between chromosome number and complexity. C- Mitosis must differ from organism to organism. Simpler have more DNA than more LO Bl.2.03 In a Mendelian monohybrid cross, which generation is always completely homozygous? AF 1 generation BF 2 generation CF 3 generation DP generation LO Bl.2.03 A cross between two true breeding lines one with .dark blue flowers and one with bright white flowers produces F 1 offspring that are light plhe. When the F 1 generation is mated a 1 :2:1 ratio of dark blue to light blue to white flowers is observed. What genetic phenomenon is consistent with these results? AEpistasis BIncomplete dominance CCo dominance lnbreedi LO Bl.2.03 A gene showing co-dominance: AHas both alleles independently expressed in the heterozygote BHas one allele dominant to the other CHas alleles tightly linked on the same chromosome Has alleles expressed at the same time in development D- BIOLOGY· Grade 11 Page 5 of 16 Scanned by CamScanner IIIII :J fj,IC -21- 8 i . cros~ed WI·than individual with a If an individual with a dominant phenotype IS recessive phenotype, 4 of their 9 offspring show the recessive phenotype. What is the genotype of the first parent? AB- CD- AA Aa aa AA or Aa I : II' jE : lllej:Jfjlll . Unattached earlobes (EE or Ee) are dominant over attached earlobes (ee). A couple both h ave unattached earlobes. Both notice that one of their parents on both sides has attached earlobes (ee). Therefore, they correctly assume that they are carriers for attached earlobl!s (Ee). The couple proceed to have four children. A 8 C D I Th~y can be certain that three will be heterozygous and one homozygous rcc esstve. If the first three are heterozygous, the fourth must be homozygous recessive. All children must have unattached earlobes since both parents possess the dominant gene for it. .. Two heterozygous, one homozygous recessive and one homozygous domina nt is a likely outcome, but all heterozygous, or two, three or all four homozygous are also possible. QUESTION 23-25 In Zea mays, the allele for coloured seed (C) is dominant over the allele for colourless seed (c). The allele for starchy endosperm (S) is dominant over the allele for waxy endosperm (s ). Pure breeding plants with coloured seeds and starchy endosperm were crossed with pure breeding plants with colourless seeds and waxy endo~erm . cState .: ,tJc :>c: : = ''''':''l"t: the genotype of the F 1 individuals produced as a result of this cross: ABC0- ' SSCC SsCc Sscc sscc ·24 • _ _ _...._......... ... ~ - · I LO B l . 2 . 0 3 In colorless seed with waxy endosperm parent, the percentage of the gametes that carries the allele of dominance · A- 12 BC0- y. % zero BIOLOGY • Gr~d~ 11 Scanned by CamScanner LO BI.2.03 The observed percentages of phenotypes in the F2 generation are shown below. coloured starchy 126 colourless starchy 44 coloured waxy42 colourless waxy 14. What is the percentage of the F2? A3:3:3:3 B9:3:3: I Cl : l : 1: 1 D3:1 LO Bl.2.03 Human blood types are genetically detennined. The table below shows the symbols used to represent two of the alleles for blood types and gives a description of each allele. Symbol I" •• Allrle Dncrlption produc~ antigen A on roo bJoOO c~lls produces antigen B on roo blood Q?lll Two Alleles Controlling Human Blood Type In homozygous individuals, two lA alleles result in blood type A and two IB alleles result in blood type B. The IA and IB alleles are co-dominant, resulting in blood type AB in individuals heterozygous for the two alleles. A male and a female both have blood type AB. If they have a child, what is the probability that the child will also have blood type AB? A1/4 B1/2 C3/4 DIll 27 I LO BI.2.03 In the fruit fly Drosophila melanogaster, vestigial wings and hairy body are produced by two recessive genes located on different chrom.os~mes. The normal alleles, long wings and hairless body, are dominant. They givelOO% Fl progeny obtained from a cross between a vestigial-winged, hairy male and a normal, homozygous female. If the Fl from this cross are permitted to mate randomly among themselves, -What phenotypic ratio would be expected in the F2 generation? 9 normal long wing, hairless body: 3 vestigial wing, hairless body: 3 nonnal Along wing, hairy body: 1 vestigial wing, hairy body 3 nonnal long wings ,hairless body : 3 normal long wing , hairy body B: I vestigial wings hairless body : 1 vestigial wings ,hairy body 1normal long wing, hairless body: I vestigial wing, hairless body C: 1 normal long wing, hairy body: 1 vestigial wing, hairy body D100% nonnal long wings ,hairless body BIOLOGY - Grade 11 Page 7 of 16 Scanned by CamScanner LO 81.2.04 your answer to the following question on the pedigree chart below, which shows a history of ear lobe shape KEY E=Allele for free ear lobes (dominant) e=Allele for attached ear lobes (recessive) 0 =Male with free ear lobes 0 =Female with free ear lobes I =Male with attached ear lobes I =Female with attached ear lobes What could the genotype of individual 1 be?. AEE, only BEe, only . Cee DEE or Ee Study the following pedigree to Answer question 29&30 LO BI.2.0" Deduce what is the type of inheritance of this disease AAutosomal recessive BAutosomal Dominance X- linked recessive trait CDLinked trait LO 81.2.0-' From the previous pedigree, the individual (13) genotype could be AXaX3 only BXA XA only CCould be xAxu or XAXA DXAX11 only BIOLOGY- Grade 11 Page 8 of 16 Scanned by CamScanner / ' -Jl- LOBI.2.0 4 - Using the pedigree below to deduce the type of inheritance in this family A- 8CD- - Autosomal recessive Sex-linked recessive Autosomal dominant Linked gene :n - LO 81.2.0-t Scientist study the genome of bacteria in termites digestive tracts , to find answer about breaking down of cellulose to produce ethanol , this is an example of applied genome in field of AAlternative energy 8Ecosystem biodiversity CEvolution DHuman Health LOBI.2.0 . t - - 3.l - How does the X chromosome differ from the Y chromosome in humans? A- The Y chromosome is longer. 8- Some genes on the X chromosome are absent from the Y chromosome. C- The genes are the same but some on the Y chromosome are not expressed. D- The X chromosome determines sex. BIOLOGY - Grade 11 Page 9 of 16 Scanned by CamScanner Key 0 0 =affected = unaffected LO BI.2.04 According to the pedigree diagrammed above the original parents of this family tree had: A3 daughters and 2 sons B2 daughters and 3 sons C2 daughters and 1 son 1 dau and 2 sons LO 81.2.04 Which of the statements below is/are true regarding the trait of interest in the above pedigree? If it is autosomal recessive, all the chlidren of the affected daughter would be Aaffected as well. If it is X-linked recessive, all the children of the affected daughter would be af Bfected. It is likely autosomal dominant; the affected individuals would be heterozygo Cus. All of these statements are true. DLO Bl.2.04 Identify the correct order of organization of genetic material, from largest to smallest. AGene, chromosome, nucleotide, genome Chromosome, gene, genome, nucleotide Chromosome, genome, nucleotide, gene Genome, chromo nucleotide LO 81.2.04 Scientist study the genome of bacteria in termites digestive tracts , to find answer about breaking down of cellulose to produce ethanol , this is an example of applied genome in field of AAlternative energy 8Ecosystem biodiversity Evolution CHuman Health DBIOLOGY - Grade 11 Page 10 of 16 Scanned by CamScanner ./ / During which phase of the cell cycle d oes DN . . LO Bl.2.05 A rephcanon occur? I ABC- Prophase· Metaphase Anaphase LO B1.2.05 The sequence of one strand of DNA is 5 TCGA TG 3 . The sequence of the complementary strand would be. A3' AGCTAC 5' B5' TCGATC 3' C5' CTAGCT 3' D3'GCTAGC 5' LO BI.2.05 IN a DNA sample, the percentage of thymine is 20% .Then what will be the percentage of guanine? A20% B40% C- Starting with N (heavy) DNA and after 2 generation in N medium . E.coli cell will contain 14 15 15 14 14 Aa. 25% N 15 N DNA, 50% N N DNA ,and 25% N N DNA 14 14 Bb. 50% N 15 N 15 DNA and 50% N N DNA Cc. 50% N 15 N 15 DNA and 50% N 15N 14 DNA 14 14 14 Dd. 50% N 15 N DNA and 50% N N DNA BIOLOGY- Grade 11 Page 11 of 16 Scanned by CamScanner . . tions (42-44} on the diagram bclo"v which atld 011 your knowledge of Base your answer to the followmg gucs . of a doubl e-s tranded DNA mo 1ecu 1e ' represents a portton biology If I LO 81.2.05 The base sequence of strand II is most likely: A3' C-A-C-T-G-G 5 ' B3' G-G-T:.C-A-C5 ' CS'G-T-G-A-C-C3' . D3' G-T-G-A-C-CS' LO BI.2.05 Which carbon in molecule No.3 will bind to the nitrogenous base ( C): ACarbon No:4 BCarbon No:3 CCarbon No: 5 DCarbon No: 1 I LO BI.2.05 When bonded together chemically, deoxyribose, phosphate, and an adenine molecule make up ADNA nucleotide BRNA nucleotide CDNA molecule DAn RNA molecule I LO BI.2.06 1. Which of the following best describes the formation of a zygote? AB- CD- A sperm cell nucleus and an egg cell nucleus fuse. A cell's DNA replication and mitosis are accelerated. A succession of cell divisions produces a solid mass of cells. A cel1 with 46 chromosomes divides tofom1 cells with 23 chromosomes each. BIOLOGY- Grade 11 Page 12 of 16 Scanned by CamScanner LO 81.2.06 Base your answer to .the. fall owmg · question · 011 The d1agram . . below shows a process that can occur dunng meiOSIS The most likely result of this process is Aa new combination of inheritable traits that can appear in the offspring Ban inability to pass either of these chromosomes on to offspring . a loss of genetic information that will produce a genetic disorder in the offspn Cng an increase in the chromosome number of the organism in which this process Doccurs LO BI.2.06 3. Which diagram illustrates fertilization that would most likely lead to the development of a abnormal human female? A) C) @+~ @+~ B) D) @+~ @+~ a AB- b C- c D- d LO 81.2.06 Hemophilia is an X-linked recessive disorder. A daughter can be afflicted with hemophilia if she inherits . . .. , ·., r Atwo normal X chromosomes; hemophilia usually arises spontaneously Ban Xh allele from her mother only. an Xh allele from her father and her mother CDan Xh allele from her father only BIOLOGY- Grade 11 Page 13 of 16 Scanned by CamScanner LO 81.2.06 4. Wild type fmit flies have red eyes. A white-eyed female fly is crossed with a red-eyed male fly. All of the females from the cross are red-eyed and all of the males, white-eye d. What type of inheritance pattern is this? AAutosomal dominant BAutosomal recessive CIncomplete dominance DSex-linked on X chromosome LO BI.2.06 If a female inherits and expresses an X-linked recessive disorder, what must be true about her parents? ABoth parents must have the disease BHer must father has the disease. CNeither parent has the disease. DHer mother has the disease BEST OF LUCK BIOLOGY- Grade 11 Page 14 of 16 Scanned by CamScanner