Biology Lesson 9.2: Photosynthesis: An Overview OBJECTIVES: 1. Describe the role pigments play in the process of photosynthesis. 2. Describe what electron carrier molecules do. 3. Identify the reactants and products of photosynthesis. CHLOROPHYLL & CHLOROPLASTS ➢ Light: sunlight is a mixture of different wavelengths of light ○ Wavelengths that are visible to our eyes are known as the visible spectrum. ➢ Pigments: light-absorbing compounds ○ Photosynthetic organisms use pigments to capture the energy in sunlight ○ Chlorophyll: principal pigment of green plants ■ Chlorophyll doesn’t absorb light well in the green region of the spectrum, so it reflects it instead. CHLOROPHYLL & CHLOROPLASTS ➢ Chloroplasts ○ Photosynthesis takes place inside organelles called chloroplasts ○ Chloroplasts are surrounded by two envelope membranes and they are filled with saclike chlorophyll-containing membranes called thylakoids ■ Thylakoids are interconnected and arranged in stacks known as grana ■ Stroma: fluid portion of the chloroplast, outside the thylakoids CHLOROPHYLL & CHLOROPLASTS ➢ Energy collection ○ Chlorophyll transfers light energy to its own electrons ■ Then these highenergy electrons are available to do chemical work HIGH ENERGY ELECTRONS ➢ High-energy electrons produced by chlorophyll are highly reactive and require a special “carrier” ○ An electron carrier is a compound that can accept a pair of high-energy electrons and transfer them, along with most of their energy, to another molecule HIGH ENERGY ELECTRONS ➢ Example: NADP+ accepts and holds two highenergy electrons, along with a hydrogen ion (H+). ■ This converts NADP+ into NADPH and NADPH can carry the high-energy electrons that were produced by light absorption in chlorophyll to chemical reactions elsewhere in the chloroplast. ■ The high-energy electrons can help build sugars like glucose from nothing more than carbon dioxide and water. AN OVERVIEW OF PHOTOSYNTHESIS ➢ Photosynthesis uses the energy of sunlight to convert water and carbon dioxide (low-energy reactants) into high-energy sugars and oxygen (products) AN OVERVIEW OF PHOTOSYNTHESIS ➢ Light-dependent reactions: first set of reactions that happen during photosynthesis ○ Requires direct involvement of light and light-absorbing pigments ○ Takes place in thylakoid membranes and uses energy from sunlight to add a third phosphate to ADP to make ATP ○ Light-dependent reactions also take low-energy electrons from water molecules and uses solar energy to raise them to a much higher energy level ■ High-energy electrons are then transferred to the electron carrier NADP+, which is converted to NADPH AN OVERVIEW OF PHOTOSYNTHESIS ➢ Light-independent reactions: second set of reactions that happen during photosynthesis ○ Plants absorb carbon dioxide from the atmosphere and complete the process of photosynthesis by producing carbon-containing sugars and other carbohydrates ○ No light is required to power the light-independent reactions, which take place outside the thylakoids, in the stroma of the chloroplast ➢ Light-dependent and light-independent reactions work together to capture the energy of sunlight and transform into energy-rich compounds such as carbohydrates