BC-5150/BC-5000
AUTO HEMATOLOGY ANALYZER
SERVICE MANUAL
Introduction
Before starting to service this instrument, please read this manual thoroughly for proper
service.
After reading this manual, make sure it is well kept so that it is available when needed.
Product Name: Auto Hematology Analyzer
Model: BC-5150/BC5-5000
Registered Address: F1-4, Mindray Building, Keji 12th Road South, Hi-tech Industrial Park,
Nanshan, Shenzhen
Production Address: Building A3, Honghualing Industrial Park, Liuxian Road, Nanshan,
Shenzhen
Intellectual Property Statement
SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS CO., LTD. (hereinafter called Mindray)
owns the intellectual property rights to this manual and the relevant product.
© 2012 Shenzhen Mindray Bio-medical Electronics Co., Ltd. All rights Reserved.
Release, amendment, reproduction, distribution, rent, adaption and translation of this
manual in any manner whatsoever without the written permission of Mindray is strictly
forbidden.
,
,
,
are registered trademarks or trademarks
owned by Mindray.
Microsoft Corporation owns the copyright of SQL SERVER 2005 EXPRESS EDITION. ©2005
Microsoft Corporation. All rights reserved.
Statement
Mindray is responsible for safety, reliability and performance of this product only in the
condition that:
all installation operations, expansions, changes, modifications and repairs of this
product are conducted by Mindray authorized personnel;
all replacement parts and supporting accessories and consumables involved in the
service are original Mindray parts or Mindray authorized parts;
Ⅰ
the relevant electrical installation complies with the applicable national requirements;
the product is operated in accordance with this service manual.
Repair Service
Free Service:
Free service is provided for any product within the scope specified by Mindray warranty
rules.
Paid Service:
Mindray offers paid service for any product beyond the scope specified by Mindray
warranty rules;
even during the warranty period, only paid service is available if the need for service is caused
by the following reasons: artificial damage; improper use; grid voltage beyond the specified
range of the device; irresistible natural disasters; replacement with parts and consumables not
authorized by Mindray or service work by personnel not authorized by Mindray.
Return Procedure
In the event that it becomes necessary to return this product or part of this product to
Mindray, the following procedure should be followed::
Obtain return authorization: Contact the Mindray Service Department and obtain a
Customer Service Authorization (Mindray) number. The Mindray number must appear
on the outside of the shipping container. Returned shipments will not be accepted if the
Mindray number is not clearly visible. Please provide the model number, serial number,
and a brief description of the reason for return.
Freight policy: The customer is responsible for freight charges when shipping the
product to Mindray for service (including customs charges).
Company Contact
Manufacturer:: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
Address: Mindray Building, Keji 12th Road South, Hi-tech Industrial Park, Nanshan, Shenzhen
Postal Code: 518057
Website: www.mindray.com
24-hour Service Hotline: 4007005652
Phone: +86 755 81888998
Fax:
+86 755 26582680
II
This system is only intended for use by qualified service personnel trained by
Mindray or Mindray's agents.
It is important for the hospital or organization that employs this equipment to
carry out a reasonable service/maintenance plan. Neglect of this may result in
machine breakdown or injury of human health.
Be sure to operate the analyzer under the situation specified in this manual;
otherwise, the analyzer will not work normally and the analysis results will be
unreliable, which would damage the analyzer components and cause personal injury.
This manual is only provided for qualified service personnel trained by Mindray or
Mindray's agents.
III
Table of Contents
1
1.1
1.2
1.3
1.4
1.5
1.6
Overview .................................................................................................................... 1-1
Overview ..................................................................................................................... 1-1
Who Should Read This Manual .................................................................................. 1-1
How to Find Information ............................................................................................ 1-1
Conventions Used in This Manual .............................................................................. 1-2
Special Terms Used in This Manual............................................................................ 1-2
Symbol ........................................................................................................................ 1-4
2
Specifications ............................................................................................................. 2-1
2.1. Product Name .............................................................................................................. 2-1
2.1.1 Physical Specifications ....................................................................................... 2-2
2.1.2 Electrical Specifications ..................................................................................... 2-2
2.1.3 Environment Requirements ................................................................................ 2-3
2.1.4 Specifications ..................................................................................................... 2-3
2.2. Testing Parameters ...................................................................................................... 2-3
2.3. Performance Requirements ......................................................................................... 2-5
2.3.1 Background/Blank Count ................................................................................... 2-5
2.3.2 Carryover ........................................................................................................... 2-5
2.3.3 Repeatability ...................................................................................................... 2-6
2.3.4 Linearity Range .................................................................................................. 2-7
2.4. Display Range ............................................................................................................. 2-8
2.5. Product Description..................................................................................................... 2-8
Main unit ................................................................................................................... 2-12
Power/Status indicator............................................................................................... 2-12
Power switch ............................................................................................................. 2-12
[Aspirate] key ............................................................................................................ 2-13
USB port ................................................................................................................... 2-13
2.6. Product Configuration ............................................................................................... 2-13
2.7. Reagents, Controls and Calibrators ........................................................................... 2-13
2.7.1 Reagents ........................................................................................................... 2-14
2.7.2Reagent Consumption Volume .................................................................................... 2-14
2.7.3 Controls and Calibrators................................................................................... 2-15
2.8. Information Storage Capacity ................................................................................... 2-15
3
Operation Principles ................................................................................................. 3-1
Overview ..................................................................................................................... 3-1
Workflow..................................................................................................................... 3-1
Sample aspiration ........................................................................................................ 3-2
White Blood Cell Measurement .................................................................................. 3-2
Laser flow cytometry .................................................................................................. 3-2
3.1
3.2
3.3
3.4
1
Table of Contents
3.5
Hemoglobin Concentration Measurement................................................................... 3-3
Colorimetric method ................................................................................................... 3-3
Hemoglobin concentration parameters ........................................................................ 3-3
Impedance method ...................................................................................................... 3-3
Time Measurement ...................................................................................................... 3-4
Red blood cell parameters ........................................................................................... 3-4
Platelet parameters ...................................................................................................... 3-5
3.6 Parameter Flags ............................................................................................................... 3-6
4
Software and Interface.............................................................................................. 4-1
4.1. Start-up ........................................................................................................................ 4-1
Version Check ............................................................................................................. 4-1
4.2. Login ........................................................................................................................... 4-2
4.2.1 Log in using service level username and password............................................ 4-2
4.2.2 Login Check ....................................................................................................... 4-2
4.3. Review ........................................................................................................................ 4-4
4.3.1 Stability Trend Graph ......................................................................................... 4-4
4.3.2 Trend Graph ....................................................................................................... 4-5
4.4. Calibration ................................................................................................................... 4-5
4.4.1 Calibration Factor and Transfer Factor .............................................................. 4-5
4.4.2 Calibration .......................................................................................................... 4-7
4.5. Sample Probe Debug ................................................................................................... 4-8
4.6. Temperature Calibration.............................................................................................. 4-8
4.7. Gain Calibration .......................................................................................................... 4-9
4.8. Gain Setting................................................................................................................4-11
4.9. Performance .............................................................................................................. 4-12
4.9.1 Background test................................................................................................ 4-12
4.9.2 Reproducibility................................................................................................. 4-13
4.9.3 Carryover ......................................................................................................... 4-14
4.9.4 Reference range of normal samples ................................................................. 4-14
4.10. Advance Toolbox ...................................................................................................... 4-15
4.10.1 System Configuration..................................................................................... 4-15
4.10.2 One-key Export .............................................................................................. 4-16
4.11. Software Update ........................................................................................................ 4-17
4.12. Status Indicator.......................................................................................................... 4-18
4.13. Buzzer ....................................................................................................................... 4-18
5
Data Transmission ..................................................................................................... 5-1
5.1. LIS Connection ........................................................................................................... 5-1
5.2. Data Management Software Setup .............................................................................. 5-3
5.3. Communication Error Analysis ................................................................................... 5-4
5.3.1 Physical Connection ........................................................................................... 5-4
5.3.2 Communication Setup ........................................................................................ 5-4
5.3.3 Network Firewall ............................................................................................... 5-4
2
Table of Contents
6
6.1.
6.2.
6.3.
6.4.
7
Optical System ........................................................................................................... 6-1
Introduction to the Principles of Optical System ........................................................ 6-1
6.1.1 Operation Principles ........................................................................................... 6-1
6.1.2 Beam Path of the Optical system ....................................................................... 6-2
Physical Structure........................................................................................................ 6-2
6.2.1 Overall Structure ................................................................................................ 6-2
6.2.2 Light Source Assembly ...................................................................................... 6-4
6.2.3 Flow Cell Assembly ........................................................................................... 6-4
6.2.4 Optical Substrate Assembly ............................................................................... 6-5
6.2.5 Preamplifier and Shield Shell............................................................................. 6-5
6.2.6 Shield Shell Assembly........................................................................................ 6-6
6.2.7 Scattering Detection Assembly .......................................................................... 6-6
6.2.8 Laser Control Board ........................................................................................... 6-7
Determinating the Optical System Status .................................................................... 6-7
Maintenance and Replacement of the Optical System ................................................ 6-9
6.4.1 Maintenance of the Optical System.................................................................... 6-9
6.4.2 Replacement of the Optical System ................................................................. 6-14
Fluidics ....................................................................................................................... 7-1
7.1. Measurement Flow ...................................................................................................... 7-1
7.1.1 WBC&HGB Channel ......................................................................................... 7-2
7.1.2 RBC/PLT Channel.............................................................................................. 7-4
7.2. Sample Volume ........................................................................................................... 7-4
7.3. Temperature of Fluidics .............................................................................................. 7-5
7.4. Reagent Consumption Volume .................................................................................... 7-5
7.5. Introduction to Fluidic Parts........................................................................................ 7-6
7.5.1 Mindray Valves .................................................................................................. 7-6
7.5.2 2-way Mindray Pressure-proof Valve ................................................................. 7-6
7.5.3 LVM Fluidic Valve ............................................................................................. 7-7
7.5.4 Pinch Valve......................................................................................................... 7-7
7.5.5 Liquid Filter ....................................................................................................... 7-8
7.5.6 Syringe Linkage ................................................................................................. 7-9
7.5.7 Electromagnetic Metering Pump ........................................................................ 7-9
7.5.8 preheat bath ...................................................................................................... 7-10
7.5.9 Vacuum Pump ...................................................................................................7-11
7.5.10 Sample Probe ................................................................................................. 7-12
7.5.11 Probe Wipes.................................................................................................... 7-12
7.5.12 Hydraulic pressure sensor .............................................................................. 7-13
7.5.13 1-way Valve .................................................................................................... 7-14
7.5.14 Baths............................................................................................................... 7-14
7.6. Detailed Introduction of Fluidic Structure ................................................................ 7-14
7.6.1 Sampling and dispensing channel .................................................................... 7-15
7.6.2 WBC&HGB Channel ....................................................................................... 7-15
7.6.3 RBC/PLT Channel............................................................................................ 7-17
3
Table of Contents
7.6.4 Precautions for Assembly and Service ............................................................. 7-18
7.7. Introduction to Sequences ......................................................................................... 7-19
7.7.1 Measurement sequence in WB-CBC+DIFF mode ........................................... 7-19
7.7.2 Measurement sequence in PD-CBC+DIFF mode ............................................ 7-32
7.7.3 Measurement sequence in CBC mode.............................................................. 7-33
7.7.4 Introduction to the Maintenance Sequences ..................................................... 7-33
8
8.1.
8.2.
8.3.
8.4.
8.5.
8.6.
8.7.
8.8.
8.9.
Hardware System ...................................................................................................... 8-1
Hardware System Overview........................................................................................ 8-1
8.1.1 Functional Block Diagram ................................................................................. 8-1
8.1.2 Electrical Connection Block Diagram................................................................ 8-2
8.1.3 System Troubleshooting ..................................................................................... 8-2
Digital Control Board.................................................................................................. 8-4
8.2.1 Overview ............................................................................................................ 8-4
8.2.2 Components........................................................................................................ 8-4
8.2.3 Adjustment and Tests ......................................................................................... 8-8
8.2.4 Troubleshooting.................................................................................................8-11
Analog Drive Board .................................................................................................. 8-15
8.3.1 Overview .......................................................................................................... 8-15
8.3.2 Components...................................................................................................... 8-15
8.3.3 Sockets and Indicators...................................................................................... 8-19
8.3.4 Troubleshooting................................................................................................ 8-23
Power Board .............................................................................................................. 8-26
8.4.1 Overview .......................................................................................................... 8-26
8.4.2 Replacement and Connection ........................................................................... 8-27
8.4.3 Troubleshooting................................................................................................ 8-27
Optical Boards........................................................................................................... 8-28
8.5.1 Optical Path and Optical System Workflow ..................................................... 8-28
8.5.2 Functions of Optical Boards............................................................................. 8-29
8.5.3 Troubleshooting................................................................................................ 8-31
Touchscreen Drive Board .......................................................................................... 8-33
8.6.1 Overview .......................................................................................................... 8-33
8.6.2 Components...................................................................................................... 8-33
8.6.3 Troubleshooting................................................................................................ 8-33
Indicator Board ......................................................................................................... 8-34
8.7.1 Overview .......................................................................................................... 8-34
8.7.2 Components...................................................................................................... 8-34
8.7.3 Troubleshooting................................................................................................ 8-35
Motor, Photocoupler and Microswitch ...................................................................... 8-35
8.8.1 Overview .......................................................................................................... 8-35
8.8.2 Troubleshooting................................................................................................ 8-36
Liquid sensor board ................................................................................................... 8-37
8.9.1 Overview .......................................................................................................... 8-37
8.9.2 Composition ..................................................................................................... 8-37
4
Table of Contents
8.9.3 Troubleshooting................................................................................................ 8-37
9
Heating System .......................................................................................................... 9-1
9.1. Heating System Overview........................................................................................... 9-1
9.2. Heating System ........................................................................................................... 9-1
9.2.1 Diluent Heating System ..................................................................................... 9-1
9.2.2 Optical heating system ....................................................................................... 9-3
10
Mechanical System .................................................................................................. 10-1
10.1. Mechanical System Overview................................................................................... 10-1
Front view ................................................................................................................. 10-1
Back view.................................................................................................................. 10-2
Left view ................................................................................................................... 10-4
Left view ................................................................................................................... 10-6
10.2. Components .............................................................................................................. 10-6
10.2.1 Introduction .................................................................................................... 10-6
10.2.2 Overall ............................................................................................................ 10-7
10.2.3 Main unit ........................................................................................................ 10-8
10.2.4 Panel Module (BC-5150) ............................................................................. 10-12
10.2.5 Panel Module (BC-5000) ............................................................................. 10-13
10.2.6 Syringe Assembly......................................................................................... 10-14
10.2.7 Sample Probe Assembly ............................................................................... 10-15
10.2.8 RBC count pool module ............................................................................... 10-16
10.2.9 HGB count pool module .............................................................................. 10-17
10.2.10 Pump Assembly.......................................................................................... 10-18
10.2.11 Power Backplane Assembly ....................................................................... 10-18
10.2.12 Optical System ........................................................................................... 10-20
10.2.13 Power Assembly ......................................................................................... 10-21
10.3. Removal and Installation......................................................................................... 10-21
10.3.1 Tools ............................................................................................................. 10-21
10.3.2 Preparation for Disassembly ........................................................................ 10-21
10.4. Disassembling the Main Unit .................................................................................. 10-22
10.4.1 Removing the Back plate ............................................................................. 10-23
10.4.2 Removing the Analog drive board PCBA .................................................... 10-23
10.4.3 Removing the Pinaster board or the SD card ............................................... 10-24
10.4.4 Removing the power backplate Assembly ................................................... 10-25
10.4.5 Removing the Fan Assembly........................................................................ 10-26
10.4.6 Removing the Right Panel............................................................................ 10-26
10.4.7 Removing the RBC count pool module(FRU) ............................................. 10-27
10.4.8 Removing the Ruby Red Cell Counter(D50um) .......................................... 10-28
10.4.9 Removing the HGB amp module ................................................................. 10-29
10.4.10 Removing the Liquid detect board PCBA .................................................. 10-31
10.4.11 Removing the Reagent Bottle plate cosmetic piece ................................... 10-31
10.4.12 Removing the preheat Assembly ................................................................ 10-32
5
Table of Contents
10.4.13 Removing the Electromagnet Pinch Valve Assembly ................................ 10-33
10.4.14 Removing the Right Valve Assembly ......................................................... 10-34
10.4.15 Removing the Pump ................................................................................... 10-35
10.4.16 Removing the Vacuum Chamber Assembly ............................................... 10-36
10.4.17 Removing the Top Cover ........................................................................... 10-36
10.4.18Removing the Temperature detection assembly .......................................... 10-37
10.4.19 Removing the Sampling Probe Assembly .................................................. 10-37
10.2.20 Replace the Horizontal Motor of the Sampling Probe Assembly............... 10-38
10.4.21 Replace the Vertical Motor of the Sampling Probe Assembly ................... 10-39
10.4.22 Removing the Probe wipe .......................................................................... 10-39
10.4.23 Replacing the Sample Probe ...................................................................... 10-40
10.4.24 Removing the Syringe Motor Position Sensor ........................................... 10-41
10.4.25 Removing the transducer discreteness ....................................................... 10-42
10.4.26 Removing the Panel Module ...................................................................... 10-43
10.4.27 Removing the Indicator board PCBA ........................................................ 10-43
10.4.28 Removing the 6301 touch control board PCBA ......................................... 10-44
10.4.29 Removing the Touch Panel......................................................................... 10-45
10.4.30 Removing the LCD Module ....................................................................... 10-46
10.4.31 Removing the Microswitch Assembly ....................................................... 10-46
10.4.32 Removing the Syringe ................................................................................ 10-47
10.4.33 Removing the Syringe Motor ..................................................................... 10-48
10.3.34 Removing the Syringe Motor Position Sensor Assembly .......................... 10-49
10.4.35 Removing the liquid detect assembly......................................................... 10-49
10.4.36 Removing the Electromagnetic Pump/Valve from the Front Panel............ 10-50
10.4.37 Removing the Left Door Assembly ............................................................ 10-51
10.4.38 Removing the Optical System .................................................................... 10-52
10.4.39 Removing the Optical System Cover ......................................................... 10-52
10.4.40 Replacing the RBC/WBC Isolation Chamber Filter .................................. 10-52
10.4.41 Replacing the Power Board PCBA ............................................................ 10-53
11
Troubleshooting ........................................................................................................11-1
12
Debug........................................................................................................................ 12-1
12.1 Mechanical Position Adjustment ................................................................................. 12-1
12.2 Commissioning of detection elements ........................................................................ 12-2
12.2.1 Calibrate and verify the preheat temperature ................................................. 12-2
12.2.2 Counting channel measurement .................................................................. 12-3
13
Commissioning and Verification After Servicing ................................................. 13-1
14
Service BOM ............................................................................................................ 14-1
15
Appendices ............................................................................................................... 15-1
6
Table of Contents
A.
Fluidic diagram ........................................................................................................ A-1
B.
Connection and Tube ................................................................................................ B-1
C.
Hardware block diagram......................................................................................... C-1
D.
Cables and Wires ...................................................................................................... D-2
E.
Appendix Table .......................................................................................................... E-1
7
1 Overview
1.1 Overview
This chapter describes how to use the service manual. In this manual, the repair methods
of BC-5150/BC-5000 are described in detail. Before servicing BC-5150/BC-5000, please carefully
read and understand the content in order to properly carry out equipment maintenance and
ensure the safety of service personnel.
This manual must be used in conjunction with the BC-5150/BC-5000 Operator’s manual. It
does not contain information and procedures already covered in the Operator’s manual of
BC-5150/BC-5000.
Be sure to operate and service the analyzer strictly as instructed in this manual and the
operator’s manual.
1.2 Who Should Read This Manual
To use this manual effectively, you need to have the following capacities:
Comprehensive knowledge of electric circuit and fluidic system;
Comprehensive knowledge of reagents;
Comprehensive knowledge of quality control;
Thorough understanding of troubleshooting;
The ability to operate the analyzer skillfully
The ability to use basic mechanical tools and understand related terminology;
The ability to use a digital voltmeter and an oscilloscope masterly;
And the ability to read pneumatic/hydraulic schematics and understand related
terminology.
1.3 How to Find Information
This manual contains 14 chapters and 4 appendices. Refer to the table below to find the
information you need.
If you want to
See
BC-5150/BC-5000's physical specifications
Chapter 2 Specifications
BC-5150/BC-5000's parameters, respective ranges and test
principle
Chapter 3 Operation Principles
interface introduction and upgrading of
BC-5150/BC-5000's software
Chapter 4 Software and
Interface
1-1
Overview
BC-5150/BC-5000's external interface settings
Chapter 5 Data Transmission
learn about the optical system of BC-5150/BC-5000's and
Chapter 6 Optical System
its maintenance methods
composition, dosage, basic channels and time sequence of
BC-5150/BC-5000's fluidic system
Chapter 7 Fluidic System
BC-5150/BC-5000's hardware structure; composition,
commissioning and testing point and troubleshooting of
each board
Chapter 8 Hardware System
BC-5150/BC-5000's heating principle
Chapter 9 Heating System
BC-5150/BC-5000's error and troubleshooting
Chapter 10 Troubleshooting
BC-5150/BC-5000's structure, disassembly and
verification
Chapter 11 Mechanical System
Debug after BC-5150/BC-5000 being serviced
Chapter 12 Debug
Debug and verification after BC-5150/BC-5000 being
serviced
Chapter 13 Debug and
Verification After Servicing
BC-5150/BC-5000's service BOM
Chapter 14 Service BOM
BC-5150/BC-5000's fluidic diagrams
Appendix A Fluidic Diagrams
BC-5150/BC-5000's fluidic tube connectors
Appendix B Lists of tube
connectors
BC-5150/BC-5000's hardware diagrams
Appendix C Hardware
Diagrams
BC-5150/BC-5000's cables and wires
Appendix D Cables and Wires
1.4 Conventions Used in This Manual
This manual uses certain typographical conventions to clarify meaning in the text:
Format
Meaning
[××]
all capital letters enclosed in [ ] indicate a key name
(either on the pop-up keyboard or the external keyboard)
“××”
letters included in " " indicate text you can find on the
screen of BC-5150/BC-5000
italic letters indicate titles of the chapters that are referred
××
to
All illustrations in this manual are provided as examples only. They may not necessarily
reflect your analyzer setup or data displayed.
1.5 Special Terms Used in This Manual
You will find the following symbols in this manual.
When you read <
It means <
1-2
Overview
read the statement below the symbol. The statement is alerting
you to a potentially biohazardous condition.
read the statement below the symbol. The statement is alerting
WARNING
you to an operating hazard that can cause personnel injury.
read the statement below the symbol. The statement is alerting
CAUTION
you to a possibility of analyzer damage or unreliable analysis
results.
NOTE
read the statement below the symbol. The statement is alerting
you to information that requires your attention.
All the substances (samples, controls, calibrators, reagents and liquid wastes)
and areas in contact with these substances are potentially infectious. Wear proper
personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory
procedures when accessing these substances and areas in the laboratory.
In the event of main unit leak, the leaking fluid is biohazardous.
WARNING
It is important for the hospital or organization that employs this equipment to carry out a
reasonable service/maintenance plan. Neglect of this may result in machine breakdown or
injury of human health.
Never use combustible gas (e.g. anesthetic) or combustible liquid (e.g. ethanol) around the
analyzer. Otherwise, the risk of explosion may exist.
When servicing the analyzer, be sure to turn off the power. Servicing the analyzer when it
is on may bring risk of electric shock or damage to electronic components.
Please connect the analyzer to a socket having sole fuse and protective switch. Do not use
the same fuse and protective switch with other equipment (e.g. life supporting equipment).
Otherwise, the equipment failure, over current or impulse current that occurs at the startup
moment may lead to tripping.
To prevent personal injury during the maintenance, keep your clothes, hairs and hands
from the moving parts, such as sample probe, pincher and piercer.
Possible mechanical movement of the warned position may lead to personal injury during
normal operation, removal and service verification.
Be sure to dispose of reagents, waste, samples, consumables, etc. according to government
regulations.
1-3
Overview
The reagents are irritating to eyes, skin and diaphragm. Wear proper personal protective
equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory procedures when
handling them in the laboratory.
If the reagents accidentally spill on your skin, wash them off with plenty of water and if
necessary, go see a doctor; if the reagents accidentally spill into your eyes, wash them off
with plenty of water and immediately go see a doctor.
CAUTION
Improper maintenance may damage the analyzer. Maintain the analyzer strictly as
instructed by the service manual and inspect the analyzer carefully after the maintenance.
For problems not mentioned in the service manual, contact Mindray customer service
department for maintenance advice.
To prevent personal injury or damage to equipment components, remove metal jewelry
before maintaining or servicing electronic components of the equipment.
Electrostatic discharge may damage electronic components. If there is a possibility of ESD
damage with a procedure, then do that procedure at an ESD workstation, or wear an
antistatic wrist strap.
NOTE
The operator is required to follow the instructions below this symbol. The
instructions will emphasize important information or information that requires
particular attention of the operator.
1.6 Symbol
Symbols used in this service manual:
Symbol
Meaning
The operator is required to follow the instructions below
this symbol. Failure to do so may place the operator at a
potential risk of biohazard.
WARNING
CAUTION
The operator is required to follow the instructions below
this symbol. Failure to do so may cause personal injury.
The operator is required to follow the instructions below
this symbol. Failure to do so may cause malfunction or
damage of the product or affect the test results.
1-4
Overview
NOTE
The operator is required to follow the instructions below
this symbol. The instructions will emphasize important
information or information that requires particular attention
of the operator.
The analyzer system may contain the following symbols:
CAUTION
Ensure the labels are in good condition and not damaged while servicing the analyzer.
When you see
It means
CAUTION, CONSULT ACCOMPANYING
DOCUMENTS.
Note: It is recommended that the reader
refers to the accompanying documents for
important safety information.
BIOLOGICAL RISK
WARNING, LASER BEAM
PROTECTIVE EARTH (GROUND)
USB port
Network interface
ALTERNATING CURRENT
FOR IN VITRO DIAGNOSTIC USE
Batch code
1-5
Overview
USE BY (YYYY-MM-DD)
Serial number
MEASUREMENT AUTHORIZATION
SYMBOL
DATE OF MANUFACTURE
Pricking danger
Manufacturer
TEMPERATURE LIMITATION
CONSULT INSTRUCTIONS FOR USE
The device fully complies with requirements
of EU IVD Directive 98/79/EC
This electronic product contains certain toxic
substances, and has an Environmental
Protection Use Period (EPUP) of 20 years. It
can be used safely during the EPUP, but
shall be recycled after the EPUP.
1-6
2 Specifications
2.1. Product Name
Name: Auto Hematology Analyzer
Model: BC-5150/BC-5000
BC-5150 Appearance
BC-5000 Appearance
2-1
Specifications
2.1.1 Physical Specifications
Height
Depth
Width
Table 2-1
Dimensions and weight
Overall
BC-5150/BC-5000
Width ≤ 325 mm
Height ≤ 435 mm (rubber feet
Dimensions
included)
Depth ≤ 410 mm
Weight
≤25Kg
2.1.2 Electrical Specifications
Table 2-2
Main unit power supply
Parameter
Voltage
Value
(100V-240V~) ±10%
≤300VA
Input Power
50/60±1Hz
Frequency
2-2
Specifications
Only fuses of specified specification shall be used.
Fuse Specification: 250V
3.15A
D5X20
2.1.3 Environment Requirements
Operating environment, storage environment and running environment
Table 2-3
Overall environment requirements
Operating Environment
Storage Environment
Running Environment
Requirements
Requirements
Requirements
10℃～30℃
-10℃～40℃
10℃～35℃
20%～85%
10%～90%
10%～90%
70kPa～106kPa
50kPa～106kPa
70kPa～106kPa
Ambient
Temperature
Relative
Humidity
Atmospheric
Pressure
2.1.4 Specifications
Measurement mode
Two measurement modes are provided: CBC and CBC+DIFF.
Sample mode
Three sample modes are provided: whole blood mode, prediluted mode and Capillary Whole
Blood mode.
Each of the three sample modes can be used in both CBC and CBC+DIFF measurement mode.
Measurement speed
BC-5000's testing speed for OV-WB/OV-PD/OV_PWB modes is not lower than 40 samples
per hour.
BC-5150's testing speed for OV-WB/OV-PD/OV_PWB modes is not lower than 60 samples
per hour.
2.2. Testing Parameters
The analyzer provides quantified results for 25 report parameters and 4 research
parameters(as shown in the table below), 3 histograms and 1 scattergram, and 2 measurement
modes (CBC and CBC+DIFF).
Parameter Series
English Name
Abbreviation
2-3
CBC
CBC + DIFF
Specifications
White blood cell parameters (15 items), including 4 research parameters
White Blood Cell count
WBC
√
√
Basophils number
Bas#
/
√
Basophils percentage
Bas%
/
√
Neutrophils number
Neu#
/
√
Neutrophils percentage
Neu%
/
√
Eosinophils number
Eos#
/
√
Eosinophils percentage
Eos%
/
√
Lymphocytes number
Lym#
/
√
Lymphocytes percentage
Lym%
/
√
Monocytes number
Mon#
/
√
Monocytes percentage
Mon%
/
√
Abnormal Lymphocytes
ALY#
/
√
ALY%
/
√
LIC#
/
√
LIC%
/
√
Red Blood Cell count
RBC
√
√
Hemoglobin Concentration
HGB
√
√
Mean Corpuscular Volume
MCV
√
√
Mean Corpuscular
MCH
√
√
MCHC
√
√
RDW-CV
√
√
RDW-SD
√
√
Hematocrit
HCT
√
√
Platelet count
PLT
√
√
Mean Platelet Volume
MPV
√
√
Platelet Distribution Width
PDW
√
√
Plateletcrit
PCT
√
√
Platelet larger cell ratio
P-LCR
√
√
Platelet larger cell count
P-LCC
√
√
number
Abnormal Lymphocytes
percentage
Large Immature Cells
number
Large Immature Cells
percentage
Red blood cell parameters (8 items)
Hemoglobin
Mean Corpuscular
Hemoglobin Concentration
Red Blood Cell Distribution
Width - Coefficient of
Variation
Red Blood Cell Distribution
Width - Standard Deviation
Platelet
parameters (6 items)
Histogram
English Name
Abbreviation
CBC
CBC + DIFF
White Blood Cell Histogram
WBC Histogram
√
√
Red Blood Cell Histogram
RBC Histogram
√
√
2-4
Specifications
Platelet Histogram
PLT Histogram
√
√
Scattergram
Name
Differential Scattergram
Abbreviation
CBC
CBC + DIFF
Diff Scattergram
/
√
“√”means available in this measurement mode, and “/”means not available in
this measurement mode.
ALY%, LIC%, ALY# and LIC# are research parameters which are only intended
for research purpose and can not serve as basis for clinical diagnosis. For more
information on research parameters, please refer to 5.2.2 General Settings Research
Parameters.
2.3. Performance Requirements
2.3.1 Background/Blank Count
Background refers to the background count performed automatically by the analyzer
during the startup process; its result shall meet the requirements in the following table.
The blank count requirements apply to both whole blood and predilute modes.
Blank count test method: run diluent on the analyzer for 3 times in a row, the highest value
among the 3 results shall meet the requirements in the following table.
Table 2-4 Background/blank count requirements
Parameter
Background/blank count requirements
≤ 0.20× 109 / L
WBC
RBC
≤ 0.02× 1012/ L
HGB
≤1g/L
HCT
≤ 0.5 %
PLT
≤ 10 × 109 / L
2.3.2 Carryover
Carryover refers to the transfer of blood cells from high concentration sample to low
concentration sample.
Verification method:
Prepare a high concentration sample (centrifugated high value control or
2-5
Specifications
special high value linearity control) which is within the range specified in Table
13, mix and then test it for 3 consecutive times, the test results are i1, i2 and
i3; prepare a low concentration sample (diluted low value control, dilution ratio:
1:10) which is within the range specified in Table 13, test it for 3 consecutive
times, the test results are j1, j2 and j3. Calculate the carryover according to
the following equation, the result shall meet the requirements in Table 12.
Table 2-5 Carryover Requirements
Parameter
Carryover
WBC
≤0.5％
RBC
≤0.5％
HGB
≤0.6％
HCT
≤0.5％
PLT
≤1.0％
Table 2-6 Sample Concentration Range of Carryover Test
Parameter
Unit
WBC
×10 /L
RBC
HGB
HCT
PLT
High concentration
range
> 15.00
9
12
×10 /L
g/L
9
×10 /L
9
×10 /L
Low concentration
range
< 3.00
> 6.00
< 2.00
> 200
< 40
> 54%
<18%
> 300
< 100
2.3.3 Repeatability
Test a sample which meets repeatability requirement on the analyzer for 10 consecutive times,
calculate the CV(%) and absolute deviation (d) of each parameter, the results shall meet the
requirements in the following table.
In the equation:
s ---- standard deviation of sample test results;
x ---- mean value of sample test results;
xi ---- actual test result of the sample;
d ---- absolute deviation of the sample test results.
2-6
Specifications
Table 2-7
Whole Blood Repeatability Requirements
Condition
Parameter
Whole
Blood
Predilute
Repeatability
Repeatability
(CV/absolute
(CV/absolute
deviation d)
deviation d)
9
4.00×10 /L～15.00× 10 /
9
≤2.0％
≤4.0％
WBC
L
Neu%
50.0％～70.0％
±4.0(d)
±8.0(d)
Lym%
20.0％～40.0％
±3.0(d)
±6.0(d)
Mon%
5.0%～10.0％
±2.0(d)
±4.0(d)
Eos%
2.0％～5.0％
±1.5(d)
±2.5(d)
Bas%
0.5％～1.5％
±0.8(d)
±1.2(d)
3.50 × 1012 / L ~ 6.00 × 1012 /
≤1.5%
≤3.0%
≤1.5%
≤3.0%
≤1.0%
≤2.0%
100 × 10 / L ~ 149 × 10 / L
≤6.0%
≤10.0%
150 × 109 / L ~ 500 × 109 / L
≤4.0%
≤8.0%
/
≤4.0%
≤8.0%
RBC
L
HGB
110 g/L ~ 180 g/L
MCV
70 fL～120 fL
9
PLT
MPV
9
2.3.4 Linearity Range
Linearity was determined by running diluted samples. Samples of different concentrations were
tested in both whole blood and predilute modes; the slope and intercept were calculated per the linear
regression equation, and then the deviation between the theoretical value and test result was obtained,
which shall meet the requirements in the following table.
Table 2-8 Linearity Requirements
Parameter
WBC
Linearity Range
0.00 × 109/L ～ 100.00
Deviation Range (Whole
Deviation
Blood)
(Predilute)
±0.30×109/L or ±5％
±0.60×109/L or ±6％
±10％
±12％
×109/L
(for both BC-5000 and
BC-5150)
100.01 × 109/L ～
500.00×109/L
(for BC-5150 only)
2-7
Range
Specifications
RBC
0.00×1012/L～8.00×
±0.05×1012/L or ±5％
±0.10×1012/L or ±10％
1012/L
HGB
0 g/L～250g/L
±2g/L or ±2％
±4g/L or ±4％
PLT
0 × 109/L ～ 1000 ×
±10×109/L or ±8％
±20×109/L or ±16％
±12％
±20％
109/L
(for both BC-5000 and
BC-5150)
1001×109/L ~ 5000×
9
10 /L
(for BC-5150 only)
HCT
0%~67%
±2% (HCT value)
or
±3% (deviation percent)
±4%(HCTvalue) or
±6%
(deviation percent)
Note: The linearity ranges above are expressed in both absolute deviation and deviation percent,
meeting either of the ranges are OK.
2.4. Display Range
Table 2-9 Display Range
Parameter
Display Range
WBC
0.00×109/L～999.99×109/L
RBC
0.00×1012/L～18.00×1012/L
HGB
0 g/L～300g/L
PLT
0×109/L～9999×109/L
HCT
0%~80%
2.5. Product Description
BC-5150 AUTO HEMATOLOGY ANALYZER composed mainly of analysis unit,
information management unit, output unit and accessories.
The illustrations in this manual are based on BC-5150. The structures of BC-5000
and BC-5150 are basically the same.
2-8
Specifications
This analyzer is heavy and may cause personal injury if handled by only one
person. If necessary, it is recommended to use two person for handling the analyzer. It
is important to follow appropriate safety rules and use appropriate tools while
handling.
2-9
Figure 2-1 Front view of the main unit
1 ----
[Aspirate] key
2 ---- Sample probe
3 ---- Power/Status indicator
4 ---- Display
2-10
Specifications
Figure 2-2 Back view of the main unit
1 --- M-52D diluent inlet
2 --- Waste outlet
3 --- Waste sensor
4 --- Power input socket
5 --- Power switch
2-11
Specifications
Figure 2-3 Left view of the main unit
1 --- Access door
2 --- Panel Module
3 --- Left door assembly
4 --- Network interface and USB port
Main unit
The machine for analysis and data processing is the main part of the product.
Power/Status indicator
The Power/Status indicator is located in the middle of the right side of the analyzer (front
side). It tells you about the status of the analyzer including ready, running, error, sleep and
on/off, etc.
Power switch
The power switch is located on the rear side of the main unit. This switch can be used to
turn on/off the main unit.
To avoid damage, do not turn on/off the power of the analyzer continually in a short
time.
2-12
Specifications
[Aspirate] key
The [Aspirate] key is located on the middle of the right front side (the right one). You can
press the key to start the selected analysis cycle, dispense diluent or wake up the system from
sleep
USB port
There are 4 USB ports on the left side of the main unit for peripheral connection or data
transmission.
2.6. Product Configuration
The system configuration is composed mainly of main analyzer unit, accompanying
accessories and reagent system. The user can choose an optional external scanner/printer.
The USB port can be used to connect the following printer models: EPSON LQ-590K, HP
Laser Jet P1505n, HP Office Jet Pro K5300, and HP LaserJet P1606dn.
2.7. Reagents, Controls and Calibrators
As the analyzer, reagents, controls, and calibrators are components of a system,
performance of the system depends on the combined integrity of all components. You should
only use the Mindray-specified reagents, which are formulated specifically for the fluidic system
of your analyzer in order to provide optimal system performance. Do not use the analyzer with
reagents from multiple suppliers. In such use, the analyzer may not meet the performance
specified in this manual and may provide unreliable results. All references related to reagents in
this manual refer to the reagents specifically formulated for this analyzer.
Each reagent package must be examined before use. Product integrity may be
compromised in packages that have been damaged. Inspect the package for signs of leakage or
moisture. If there is evidence of leakage or improper handling, do not use the reagent.
Store and use the reagents as instructed by instructions for use of the reagents.
When you have changed the diluent, lyses or cleansers, run a background to see if the
results meet the requirement.
Pay attention to the expiration dates and open-container stability days of all the
reagents. Be sure not to use expired reagents.
After installing a new container of reagent, keep it still for a while before use.
2-13
Specifications
2.7.1 Reagents
M-52 D DILUENT
Used for diluting the blood samples to achieve functions such as blood cell counting,
volume measurement and hemoglobin measurement.
M-52 DIFF LYSE
Used for lysing red blood cells to categorize quartile groups of white blood cells.
M-52 LH LYSE
Used for lysing red blood cells to achieve functions such as white blood cell counting,
classification of basophils and hemoglobin measurement.
PROBE CLEANSER
Used for cleansing the instrument regularly.
2.7.2Reagent Consumption Volume
Table 2-10 3107 reagent volume
Diluent
Sample Mode
DIFF Lyse (ml)
LH Lyse (ml) Probe cleanser
(ml)
(ml)
CD
27.5
1
0.2
0
CBC
23.2
0
0.2
0
CD
27.5
1
0.2
0
CBC
23.2
0
0.2
0
CD
27.6
1
0.2
0
CBC
22.9
0
0.2
0
Shutdown
60.9
1
0.2
2
Normal Startup
65
1
0.2
0
Exit Standby 1
3.7
0
0
0
Exit Standby 2
16.9
0
0
0
Exit Standby 3
65
3
0.2
0
Whole Blood Mode
Capillary Blood Mode
Prediluted Mode
2-14
Specifications
2.7.3 Controls and Calibrators
The controls and calibrators are used for calibration and quality control of the analyzer.
The control is an industrial whole blood product, used for monitoring and evaluating the
accuracy of the hematology analyzer. They are available in low, normal, and high levels. The
control is also an industrial whole blood product, used for the calibration of this analyzer to
establish metrological traceability for measurement results. For use and storage of controls and
calibrators, please refer to the instructions for use of the controls and calibrators.
All references related to controls and calibrators in this manual refer to the controls and
calibrators specifically formulated for this analyzer by Mindray.
2.8. Information Storage Capacity
3.
Table 2-11
Data storage requirements
Storage
Capacity
BC-5000's storage capacity of sample data is not less than 20000
BC-5150's storage capacity of sample data is not less than 40000
Storage
Contents
The storage contents shall include at least the following
information: counting results and diagrams (including histograms
and scattergrams), sample information, patient information,
alarm message, special information of the instrument
2-15
3 Operation Principles
3.1 Overview
This analyzer employs Coulter principle to test the number of RBC and PLT, colorimetric
method to measure the hemoglobin concentration, and semiconductor laser flow cytometry
to obtain differential statistics of white blood cells. The analyzer will calculate the other
parameters based on these results.
3.2 Workflow
The whole system contains the following main functions: reagent system, sample allocation,
sample preparation, sample testing, signal processing, parameter analysis, data management,
status monitoring, scheduling control and information processing, human machine interface,
power supply, cleaning and maintenance. The relationship between these functions are as
shown in the chart below.
The scheduling control and information processing function block controls other function
blocks, which collaborate in accordance with the designed processes and requirements to
3-1
Operation Principles
complete the core task of the whole system, i.e. sample measurement and analysis.
3.3 Sample aspiration
he analyzer will aspire 15μL (in CBC+DIFF mode) or 11.5μL (in CBC mode) of whole blood
sample in the OV-WB mode.
In OV-PD mode, the operator shall mix 20 μL of capillary blood sample and 480 μL of
diluent outside the analyzer to obtain a diluted sample with the dilution ratio of 1:25, and
send this diluted sample to the analyzer. In this event, the analyzer will aspirate 200 μL of
diluted sample.
3.4 White Blood Cell Measurement
Laser flow cytometry
Figure 3-1 White blood cell measurement
After the blood sample is mixed by the lyse, the red blood cells will be lysed, and the white
blood cells will be dyed. Through the sample probe, the dyed fragments of white blood cell and
red blood cell are injected into the flow cell, which is filled with the diluent. Wrapped in the
sheath fluid formed by the diluent, the cells go through the laser detection zone in rows after a
secondary acceleration. When the cells are exposed to laser beam, the scattered light is related
to the cell size and the refractive index of both the cell membrane and the internal structure.
These scattered light signals are received and converted into electrical pulses by the photodiode.
From these electrical pulses, a two-dimensional distribution map of the cell size and internal
information and be obtained, which is called a scattergram. From the WBC scattergram and
histogram, the white blood cell differential and count can be obtained.
3-2
Operation Principles
3.5 Hemoglobin Concentration Measurement
Colorimetric method
After the diluted sample is added into the lyse, the red blood cells will lyse and release
hemoglobin, which combines the lyse to form hemoglobin complexes. According to the
Lambert-Beer's law, with the radiation of LED monochromatic light with a central wave length of
530 nm, it is possible to measure the transmitted light density of the hemoglobin complexes in
the solution and background, by which the hemoglobin concentration can be calculated.
Hemoglobin concentration parameters
The concentration of hemoglobin (HGB) in g/L can be calculated from the following
equation.
 Blank Photocurrent 

HGB(g/L) = Constant × Ln 
 Sample Photocurrent 
Red blood cell/Platelet measurement
Impedance method
This analyzer employs the impedance method to count the RBC/PLT. There is a small
opening in the RBC bath, which is called inspection aperture. A pair of electrodes on both sides
of the aperture are connected to a constant current power supply. Since the cells are poor
conductor of electricity, when the cells in the diluted sample pass through the aperture under a
constant negative pressure, the resistance between the electrodes changes to generate a pulse
signal across the electrodes, which is proportional to the cell size. The number of the pulses is
equal to the number of cells that pass the aperture, and the amplitude of the pulses is
proportional to the cell size.
3-3
Operation Principles
Figure 3-2 The counting principle
The collected electric pulses are amplified and then compared with the channel voltage
threshold corresponding to the size range of normal red blood cells/platelets, in order to
calculate the number of the pulses which amplitude are within the red blood cell/platelet
channel. Therefore, the collected electric pulses are classified according to the channel voltage
threshold. The numbers of the electric pulses within the red blood cell/platelet channel are the
numbers of the red blood cells/platelets. The size distribution of the cells is determined by the
numbers of the cells in each channel, which are classified according to the pulse voltage
amplitude. The two-dimensional diagram, in which the horizontal axis represents the cell size
and the vertical axis represents the relative number of the cells, are the histogram that reflects
the distribution of the cell groups.
Time Measurement
The basis of time measurement is under certain vacuum pressure and
with fixed aperture dimension, the sample volume passes through the
aperture within a certain period of time is definite. Thus analysis
results can be obtained by calculating sample volume from analysis
duration, dilution ratio, etc. And analysis accuracy can be ensured by
controlling vacuum generating process and monitoring real-time vacuum
pressure and aperture voltage.
Monitor and determine clogging: by monitoring aperture and sample
particle information, relevant characteristic information is concluded,
and the judgment is made in combination with the threshold.
Red blood cell parameters
Red blood cell counts
3-4
Operation Principles
RBC (1012/L) is the number of erythrocytes measured directly by counting the erythrocytes
passing through the aperture.
Mean red blood cell volume
Based on the RBC histogram, this analyzer calculates the mean cell volume (MCV) and
expresses the result in fL.
Hematocrit, mean corpuscular hemoglobin and mean corpuscular hemoglobin
concentration
This analyzer calculates the HCT (%), MCH (pg) and MCHC (g/L) as follows, where the RBC is
expressed in 1012/L, MCV in fL and HGB in g/L.
HCT =
RBC × MCV
10
MCH =
HGB
RBC
MCHC =
HGB
× 100
HCT
where the RBC is in 1012/L, the MCV is in fL, and the HGB is in g/L.
The coefficient variation of the red cell distribution width
Based on the RBC histogram, this analyzer calculates the CV (Coefficient of Variation, %) of
the erythrocyte distribution width.
The standard deviation of the red cell distribution width
RDW-SD (RBC Distribution Width – Standard Deviation, fL) is obtained by calculating the
standard deviation of the red blood cell size distribution.
Platelet parameters
Platelet count
PLT (109/L) is measured directly by counting the platelets passing through the aperture.
Mean platelet volume
Based on the PLT histogram, this analyzer calculates the mean platelet volume (MPV, fL).
Platelet distribution width
Platelet distribution width (PDW) is the geometric standard deviation (GSD) of the platelet
size distribution. Each PDW result is derived from the platelet histogram data and is reported as
10(GSD).
Plateletcrit
3-5
Operation Principles
This analyzer calculates the PCT as follows and express it in ％, where the PLT is expressed
in 109/L and the MPV in fL.
where the PLT is in 109/L and the MPV is in fL.
3.6 Parameter Flags
3.6.1 Flags
The following table lists all 22 flags and their indications.
Flag
Type
Flag
Meaning
Judgment criterion
Interference of PLT clump or 1. The DIFF and BASO
WBC Abnormal
NRBC to WBC count and channels
differential may exist:
Immature Cell?
Immature cells or blasts may
exist
Many scatter-points in the
immature cell area of the
scattergram
Abnormal
Abn./Atypical Lym?
are
unproportionate.
atypical
lymphocytes
lymphocytes
or
may
exist.
Many scatter-points in the
abnormal/
atypical
lymphocytes area of the
scattergram
Leucopenia
Low WBC analysis results
WBC < 2.50×10^9/L
Leucocytosis
High WBC analysis results
WBC > 18.00×10^9/L
Neutropenia
Low
neutrophils
analysis
NEUT# < 1.00×10^9/L
results
WBC
Neutrophilia
Lymphopenia
Lymphocytosis
Monocytosis
Eosinophilia
Basophilia
High
neutrophils
analysis
NEUT# > 11.00×10^9/L
results
Low
lymphocytes
analysis
LYMPH# < 0.80×10^9/L
results
High
lymphocytes
analysis
LYMPH# > 4.00×10^9/L
results
High
monocytes
analysis
MONO# > 1.50×10^9/L
results
High
eosinophils
analysis
EO# > 0.70×10^9/L
results
High basophils analysis results BASO# > 0.20×10^9/L
Wbc < 4.0×10^9/L and Rbc <
Pancytopenia
WBC, RBC and PLT low
3.5×12^9/L and Plt < 100
×10^9/L
RBC
RBC Histogram Abn.
Possible
presence
microcytes,
3-6
of The
distribution
of
macrocytes, histogram is abnormal
RBC
Operation Principles
anisocytosis,
RBC
agglutination
and
dimorphic
histogram
HGB
HGB Abn./Interfere?
abnormal
or
RBC
agglutination, or interference
may exist (e.g., WBC high)
MCHC > 380 g/L
or HGB interference
Microcytosis
MCV low
Mcv < 70fL
Macrocytosis
MCV high
Mcv > 110fL
Anemia
Anemia
HGB < 90g/L
Erythrocytosis
RBC high
RBC > 6.5×10^12/L
Possible
presence
PLT Scattergram Abn. microcytes,
red
blood
of
cell
debris, giant PLT or PLT clump
The
distribution
of
PLT
scattergram is abnormal
PLT
Thrombopenia
PLT low
PLT < 60×10^9/L
Thrombocytosis
PLT high
PLT > 600×10^9/L
3.6.2 Shielding Protocol
See the following table for the shielding relations of each flag.
Flag
Type
Flag
Shielding Relation
WBC Abnormal
R/？Flag parameter: WBC, related DIFF parameters
Immature Cell?
R/？Flag parameter: Neu％#, Mon％#, Bas％#.
Abn./Atypical
R/？Flag parameter: Lym％#, Mon％#, Bas％#, etc. Differ
slightly based on the situation.
Lym?
Leucopenia
/
Leucocytosis
/
Neutropenia
/
Neutrophilia
/
Lymphopenia
/
Lymphocytosis
/
Monocytosis
/
Eosinophilia
/
Basophilia
/
Pancytopenia
/
WBC
RBC
RBC Histogram Abn.
R/？Flag parameter: RBC, HCT, RDW－CV, RDW－SD, etc.
Position of the “?” differs slightly based on the situation.
HGB Abn./Interfere?
R/？Flag parameter: HGB, MCH, MCHC.
Microcytosis
/
Macrocytosis
/
3-7
Operation Principles
Anemia
/
Erythrocytosis
/
PLT Scattergram Abn. R/？Flag parameter: PLT, MPV, PDW, PCT, PLCR, PLCC, etc.
PLT
Thrombopenia
/
Thrombocytosis
/
3.6.3 Sensitivity Adjusting Mechanism
The sensitivity adjusting mechanism allows adjustment of flag rate to meet the needs of all
hospitals (see “Flag alarm sensitivity” in the Operator’s Manual).
3-8
4 Software and Interface
4.1. Start-up
Version Check
At startup, the machine will automatically check the software version. If the software version
does not match, a window will pop up as shown below:
Figure 4-1The software version match window
Please prepare the software upgrade package, select the appropriate language and click
the “OK” button. The system will perform the software upgrade progress.
Detection of unmatched software version at startup is caused by upgrading individual
software component, such as digital version FPGA chip writing software, drive board FPGA
writing software, MCU writing software, sequence and system software.
Always use controlled software upgrade package for software upgrade.
4-1
Software and Interface
4.2. Login
4.2.1 Log in using service level username and password
Username: "Service"
Password: "Se s700"(there is a space between Se and s700).
The login password is case sensitive.
4.2.2 Login Check
After login with service level password, the machine will automatically check the board backup
data and SD card data. If any board or SD card has been changed, or abnormal shutdown occurs after
configuration, a recovery or backup operation of the instrument data will be prompted after login with
service level password.
Figure 4-2Login check prompt window
Click “OK” to enter the Data Backup and Recovery screen, and perform data backup and
recovery according to the prompts.
4-2
Software and Interface
Figure 4-3 Data Backup and Recovery screen
1) If a new SD card is replaced, please follow the subsequent text guide and select “Restore” to
restore the important parameters to the SD card.
2) If the MPU is replaced, please follow the subsequent text guide and select “Backup” to back
up the data in the new MPU.
3) If abnormal shutdown occurs after configuration has been changed, please select “Backup” to
back up the data in the new MPU.
4-3
Software and Interface
4.3. Review
4.3.1 Stability Trend Graph
Figure 4-4 Stability trend graph screen
The stability and the limit of the RBC and PLT particles are considered to be 15% of the mean
by default; the stability and limit of other particles are considered to be 10% if the mean by default;
the limits of the fluids pressure, RBC aperture voltage, HGB voltage and vacuum in the vacuum
chamber are considered to be 10% of the mean by default.
The limit can be set manually; the data length is 6 digits;
If the limit is larger than the mean, the total low limit is 0.
4-4
Software and Interface
4.3.2 Trend Graph
Figure 4-5 Trend Graph Screen
If the mean of the parameter can be gained by calculation of the selected data, then the y-axis of
mean central line, upper limit data line, lower limit data line are as follow by default: Mean, Mean +
Mean * 10%, Mean – Mean * 10%;
If the results of the “Mean+ deviation” do not meet the requirements of the data format of the
parameter, then obtain the y-axis of the upper and lower data line by the rounding-off principle.
If the limit is larger than the mean, set the low limit as 0;
If the upper limit exceeds the parameter display range, click the “OK” button, the “OK” dialog
box will prompt”The upper limit exceeds the parameter display range, please reset”.
4.4. Calibration
4.4.1 Calibration Factor and Transfer Factor
The purpose of calibration is to obtain accurate blood analysis results.
The calibration method is multiplying the result by the calibration factor, so that the final
analysis result is close to the target. The calculation equation of the calibration factor is:
4-5
Software and Interface
There are two different analysis modes, CBC+DIFF and CBC. The two analysis modes
respectively correspond to two fluidics sequence. Therefore, the analysis results of the same sample in
different modes are different. However, this difference is relatively fixed. During calibration, it is
only required to obtain the calibration factor of one mode. The calibration factor of the other mode
can be calculated by multiplying this fixed difference coefficient, which is called the transfer factor.
The calculation equation of the transfer factor is:
There are two different sample modes, whole blood mode and prediluted mode, which also
correspond to different fluidics sequence. Therefore, different sample modes need to be calibrated
separately.
The calibration factors can be classified as factory calibration factor and user calibration factor.
For the CBC+DIFF mode, the analysis result will be calculated by the following equation:
Analysis result=measurement value × factory calibration factor × user calibration factor
For the CBC mode, the analysis result will be calculated by the following equation:
Analysis result=measurement value × factory calibration factor × transfer factor × user
calibration factor
The calibration will only generates calibration factors and transfer factors of five traceable
parameters: WBC, RBC, HGB, MCV and PLT.
If login with service level password, the calibration will modify the factory
calibration factor and transfer factor, and will modify the user calibration factor to
100.00%.
4-6
Software and Interface
4.4.2 Calibration
Figure 4-6Service level calibration screen
The service level calibration with calibrators will generate the factory calibration factor and
transfer factor at a time. The first 6 counts are performed in CBC+DIFF mode, and the last 6 counts
are performed in CBC mode. After all the 12 counts are completed, the new calibration factor and
transfer factor will be automatically calculated. The operator will be prompted to save the calibration
factor when exiting this screen.
Before the calibration, be sure to set up the Calibrator Lot No., the Calibrator Exp. Date,
Analysis Mode and Calibration Targets.
The range of Calibration factor is [0/75, 1.25]. .
Please use specified calibrators for calibration before their expiration date.
If the calibration factor and CV are beyond the above range, they will be displayed in
red, and the current result will not be saved.
4-7
Software and Interface
4.5. Sample Probe Debug
The purpose of sample probe debug is to check if the probe can move to each working position
properly.
Figure 4-7Sample probe debug screen
Enter the sample probe debug screen and click the “Initial position” button. Wait until the
initialization is completed before start the sample probe debug. For detailed information, please refer
to Section 12.1 “Mechanical position debug” in Chapter 12.
4.6. Temperature Calibration
The purpose of temperature calibration is to minimize the difference between the measurement
and the actual temperature in order to ensure the accuracy of sample analysis.
4-8
Software and Interface
Figure 4-8Temperature calibration screen
There are four quantities in this screen: total difference, new difference, machine measurement
value and meter measurement value. This screen does not include one quantity: the actual
measurement value, which is the actual temperature measured by the temperature sensor. These
quantities satisfy the following equation:
New difference = Meter measurement value – Actual measurement value
After clicking “Save”, the new difference is assigned to the total difference: Total difference =
New difference
Machine measurement value = Actual measurement value + Total difference
4.7. Gain Calibration
Gain calibration includes gain calibration of the optical channel, the impedance channel and
HGB. The purpose of optical channel gain calibration is to ensure the accuracy of the algorithm
categorization, and the purpose of impedance channel and HGB gain calibration is to ensure accurate
values of MCV and HGB to be calculated. If the scattergram is normal but there is no categorization
results or obvious deviation in the categorization results, check the optical system or recalibrate the
optical gain. If the HGB background voltage in the status screen is beyond the range, recalibrate the
HGB gain.
4-9
Software and Interface
Figure 4-9 Service level calibration screen
The optical gain, MCV gain and HGB gain can be calibrated simultaneously in the Gain
Calibration screen. As a software gain, the optical gain can be calibrated by mean method. Selecting
the “Select” box of the single count to involve this count in the mean calculation. As hardware gains,
the MCV gain and the HGB gain can be calibrated by successive approximation, and the “Select” box
has no effect on the results of the MCV gain and HGB gain.
Whether the gain calibration is successful is marked by color. Unsuccessful calibration result
will be displayed in red, which means a recalibration is necessary.
If a target has not been set up, then the corresponding gain factor will not be calibrated, as
G.S. MCV in the figure above.
Note: The G.S. MCV target is not the same as MCV calibration target, they are not the
same value.
When exiting the Gain Calibration screen, the operator will be prompted whether to save the
gain factor.
Only calibration with calibrators is supported in the Gain Calibration screen.
4-10
Software and Interface
Please use specified calibrators for gain calibration before their expiration date.
For the targets, please refer to the calibrator target sheet.
4.8. Gain Setting
The optical gain, MCV gain and HGB gain can be set up in the Gain Setting screen. The purpose
of gain setting and gain calibration is the same. Gain setting can be considered as manual gain
calibration.
Figure 4-10 Gain Setting screen
The optical gain is a software gain and shall be filled with the gain factor, which can be
calculated by the following equation:
The gain factor is a percentage value. The Gain Setting screen allows the gain factor to be set as
accurate to two decimal places.
The MCV gain and HGB gain are software gains, which require the digital potentiometer to be
set. The range of gain setting is [0, 255].
The MCV gain setting can be calculated by the following equation:
4-11
Software and Interface
The HGB gain setting does not need to be calculated by an equation. Just modify the
setting until the background voltage is equal to 4.5V.
The gain settings will have effect on the affectivity of the measurement. Please be
careful with the setting.
While the analyzer is in standby, the HGB voltage will not reflect the background
voltage. In this event, the operator must exit the standby mode before adjusting the HGB
gain.
4.9. Performance
4.9.1 Background test
Figure 4-11 Background Count screen
In the Background Count screen, pressing the aspirate key without using any sample will start
the background count. In the Background Count screen, if “pass” is displayed in the Result column,
4-12
Software and Interface
then the background test is passed.
4.9.2 Reproducibility
Figure 4-12 Reproducibility Test screen
Select the samples that satisfy the reproducibility range and perform 10 consecutive
measurements on the analyzer. Calculate the CV (%) or absolute deviation d of each measured
parameter and check if the reproducibility requirement is met.
Normal controls are usually used for reproducibility application at the client end.
4-13
Software and Interface
4.9.3 Carryover
Figure 4-13 Carryover Test screen
Test method: Under the stable condition of the analyzer, perform three consecutive
measurements on the high-level sample immediately followed by three consecutive
measurements on the low- level sample. The carryover can be calculated from the following
equation:
Carryover (%) =
First low - level sample result－Third low - level sample result
× 100％
Third high - level sample result－Third low - level sample result
Advanced Toolbox
4.9.4 Reference range of normal samples
Parameter
NEU_LAS_P
NEU_MAS_P
NEU_WAS_P
NEU_EQWIDTH_P
NEU_LAS_CV(%)
NEU_MAS_CV(%)
NEU_WAS_CV(%)
NEU_EQWIDTH_CV(%)
Mean
139.91
123.87
39.40
14.57
6.83
12.95
20.88
1.12
4-14
Deviation
Range
6.01
9.84
4.80
0.30
3.15
2.35
2.35
0.65
Software and Interface
4.10. Advance Toolbox
4.10.1 System Configuration
Figure 4-14System Configuration screen
In the System Configuration tab of the advanced toolbox, you can modify the language type, the
analyzer serial No. and data type to be saved.
After the “Save inf file” option is selected, the inf file will be saved to directory
/mnt/hd/soft/dat/rawdata. The inf files save the analyzer status and sample analysis data for
diagnostics of analyzer performance.
After the “Save raw data” option is selected, the dat file will be saved to directory
/mnt/hd/soft/dat/dat. The dat files save the raw pulse signals collected by the MPU FPGA for
diagnostics of analyzer performance.
The modification to language type will not take effect until after the analyzer is
restarted.
The saved inf files and raw data files will occupy relatively large amounts of disk
(SD card) space.
4-15
Software and Interface
At most 500 inf files can be saved. After 500 files are saved, the new files will
overwrite the old files.
4.10.2 One-key Export
Figure 4-15One-key Export screen
The contents that can be exported by One-Key operation include:
Inf files
Special information files
Analyzer information: includes version information, configuration parameter (gain and
calibration), algorithm parameters, analyzer status, software language and analyzer name
Software debug information: includes parameter setting, error log, upgrade log and system
log.
Closed-reagent information: includes closed-reagent information and counter information
The USB flash drive has been pre-formatted as FAT32.
The following USB drive models are recommended: Kingston 8/16G, SanDisk 8/16G,
4-16
Software and Interface
Maxell 4/8G.
There is enough free space in the USB drive. It is recommended to reserve 4G space.
4.11. Software Update
Create an upgrade USB drive
Unzip update.rar and copy the unzipped “update” directory to the root directory of the
USB drive.
The USB flash drive has been pre-formatted as FAT32.
The directory structure of USB drive: the root directory contains a directory named
update, which contains two directories: step1 and step2.
Upgrade
Insert the USB drive into the USB port on the analyzer. Enter the Advanced Toolbox
and launch Upgrade to upgrade the software according to the prompts. The upgrade process can
be divided into two steps.
Step 1: upgrade the guidance and operation system,
Step 2: upgrade the software components.
When upgrading the boot and operation system, the operator will be prompted to
restart the analyzer between step 1 and step 2. When upgrading the software components, the
upgrade process will directly go to step 2.
Never disconnect the USB drive or the power supply during the upgrade process.
Otherwise the analyzer may not be able to start.
The duration of the upgrade process varies with the upgrade contents. Typically it
will last for around 10 minutes. Interaction is needed during the process. Please do
not leave when upgrading the analyzer.
4-17
Software and Interface
Troubleshooting
If the upgrade fails, try again.
4.12. Status Indicator
The system status is indicated by the three color indicator on the panel door. All the flash cycles
are 2 seconds. The indicator changes with the analyzer status as shown in the table below:
Table 4-1 Indication of the main unit status indicator
Analyzer Status
Indicator
Comments
Ready
Green light on
Sequence is allowed
Running
Green light flashing
Sequence is being performed
Running with error
Red light flashing
An error is present and the
system is running
Stop with fault
Red light on
An error occurs and the
system is not running
No fault is present, but
Yellow light on
Initialization and standby
fluidics action is not
status of sequence is not
allowed
involved in the startup
process
Enter/Exit standby
Yellow light flashing
Enter/Exit standby status
status
4.13. Buzzer
When an error occurs, the buzzer will beep. The alarm will be automatically cleared by tapping
the touchscreen or correcting the error. The buzzer alarm will stop when all the error are cleared. It
prompts to instruct the user with possible actions by the beep.
Table 4-2 Main unit buzzer prompts
Event
Buzzer
Comments
Prompts
Startup completed
One short beep
Startup completed means the
whole startup process has been
completed and the analyzer is
ready for operation
Open-vial aspiration completed
Two short beeps
When count operation can not be
One long beep
4-18
If these screens have already
Software and Interface
started in count related screens
prompted, then the buzzer need
(including Sample Analysis, QC,
not be responded again.
Calibration, Reproducibility,
Carryover, Background, Aging and
Optical Gain Calibration Count, etc.),
press the aspirate key
Error
Analyzer is ready
Long intermittent
Tap the touchscreen to stop the
beeps
buzzer
One short beep
Analyzer enters ready status
from other status
When the screen is black prompting
Silence
“Please power off the analyzer”
If an error occurs during the
shutdown process, the buzzer
will beeping when the screen is
black
4-19
5 Data Transmission
5.1. LIS Connection
Communication setup (“Menu”>“Setup”>“System Setup”>“Communication Setup”)
The operator can perform the following setups in the “Communication Setup” screen
Protocol setup
Transmission mode
Figure 5-1 Communication Setup screen
Protocol setup
IP address:
The IP address setting of the analyzer defaulted as 10.0.0.2
Subnet mask:
The subnet mask of the analyzer. A typical subnet mask is 255.255.255.0
Default gateway:
IP address of the gateway.
Mac address:
The Mac address of the analyzer, given by the factory. Can not be changed.
Comm. Protocol:
For selecting the protocol type. Click the pull-down list and select the appropriate
communication protocol type from the options.
5-1
Data Transmission
ACK synchronous transmission:
This function can be activated by selecting the “ACK synchronous transmission”
checkbox.
When this function is active, the ACK overtime is defaulted as “10” seconds. The
operator can adjust this value in the edit box.
The IP address of the analyzer is statically allocated. Before setup, please consult
your network administrator to avoid IP conflict.
For communication across subnets, the subnet mask and the gateway must be correct.
Please consult your network administrator.
Transmission mode
The operator can select required options by clicking the following checkboxes to activate
corresponding communication setup as needed:
Auto retransmit
The “Auto retransmit” can only be selected when the “ACK synchronous
transmission” is selected, if ACK is not selected after overtime, the software will
transmitted the content that has transmitted before automatically.
Auto communication
If it is selected, the software will transmit the sample information and count
result to the LIS automatically after the sample analysis.
Transmit as print bitmap data
If it is selected, the scattergram and histogram that transmitted to the LIS are the
same as the print graph, whose background are white.
Transmission method for histograms and scattergrams
Click the pulldown list and select the transmission method for histograms and scattergrams
as required from the following options:
Not transmitted
If it is selected, the graphic and image data will not be transmitted.
Bitmap
If it is selected, the image data will be transmitted, all the scattergram and
histogram of the LIS received are bitmap.
Data
If it is selected, the graph data will be transmitted during the transmission, the
scattergram and histogram that LIS received is the data, the data format is customized;
it can only be viewed by the Mindray data management software.
5-2
Data Transmission
5.2. Data Management Software Setup
Communication parameter setup
Figure 5-2 Communication Parameter Setup screen for data management software
As illustrated above, IP Address refers to the analyzer IP address, Port is fixed as 5100, and
Protocol shall match the analyzer configuration.
Communication instrument management
5-3
Data Transmission
Figure 5-3 Communication Instrument Management screen for data management software
The analyzer is the server, and the LIS and data management software is the client. The
connection needs to be initiated by the client.
5.3. Communication Error Analysis
5.3.1 Physical Connection
Check if the network cable works properly and if the physical network connections are
correct.
5.3.2 Communication Setup
Check if the network setup is correct, including the communication setup of the analyzer
and LIS.
5.3.3 Network Firewall
Please open the network connection license of LIS and data management software, and
open the network connection license of port 5100 to check for the firewall.
5-4
6 Optical System
6.1. Introduction to the Principles of Optical System
6.1.1 Operation Principles
The basic principle of optical system is the employment of flow cytometry-based laser scattering
method. As shown in Figure 6-1, wrapped in the diluent sheath, the processed and diluted blood
forms a sample stream carrying cells. This sample stream becomes very thin due to the focusing
effect of the sheath, forcing the cells to flow one by one through the center of the chamber with a
certain space between each other. Elongated elliptical Gaussian beam goes through the optical zone of
the flow cell, irradiates individual cells to generate scattered light, which is received by the detector
and transformed into photoelectric signals necessary for cell counting and categorizing.
Figure 6-1 Laser scatter principle
Since the sample stream has a certain width, different cells will pass the beam zone at slightly
different positions. In order to ensure the consistency of cell scatter signals, the Gaussian beam shall
has a certain width in the direction perpendicular to the cell movement to minimize the density
variation of the beam coving the sample stream, as shown in Figure 6-1. Meanwhile, in order to
prevent the beam from radiating several cells at the same time, the beam shall be small enough in the
direction of the cell movement, just being able to cover the entire cell. Therefore, the beam used for
radiating the sample stream shall be an elongated elliptical beam.
6-1
Optical System
6.1.2 Beam Path of the Optical system
As a component of the optical path, the optical zone of the flow cell is the zone that the laser
beam passes which are shown as follows:
Light source assembly: Beam shaping function component, which shapes the divergent elliptical
beam generated by the semiconductor laser to elongated beam and directs it into the flow cell.
Flow cell assembly: Both an optical component and a fluidic component. As the fluidic interface
of the optical system, it provides stable sample streams. The optical performance of this component is
also critical. Dirt, contamination or dust in the optical zone on the inside and outside surface of the
flow cell may have great impact on the performance of the optical system.
Scattering detection assembly: consists of aperture and photoelectric sensor. Used for collecting
the scattered light generated by the cells. There are three photoelectric sensors in the optical system
for collecting scattered light of three angle ranges, including Low Angle Scatter (LAS), Medium
Angle Scatter (MAS) and Wide Angle Scatter (WAS).
Figure 6-2 Optical path diagram of the optical system
The relative positional relationship between the three parts of the optical path is achieved by
precise commissioning and fastening with special instruments, and therefore is not field serviceable.
6.2. Physical Structure
6.2.1 Overall Structure
The overall structure of the optical system is shown in Figure 6-3. According to different
functions, the system can be divided into the following parts:
6-2
Optical System
Figure 6-3 Overall structure model of the optical system
1 ---Light source assembly
2 ---Flow cell assembly
3 ---Substrate assembly
4 ---Preamplifier and shield shell
5 ---Shield assembly
6 ---Scattering detection assembly
7 ---Laser control board
Figure 6-4 Physical structure of the optical system
6-3
Optical System
6.2.2 Light Source Assembly
The light source assembly is used for providing light source output and beam shaping for the
optical system, as shown in Figure 6-5. Both the removal of internal parts of the light source assembly
and the removal of the assembly from the substrate are forbidden. Generally, if the light source
assembly is determined to be faulty, the whole optical system shall be replaced.
Figure 6-5 Light source assembly
6.2.3 Flow Cell Assembly
The flow cell assembly is the fluidic interface of the optical system. The fluidics are turned into
stable sheath stream under the pressure of the shield fluid bath. After reaction, the cells are injected
by the sample syringe into the flow cell assembly and are wrapped by the sheath. Then the cells go
through the flow cell one by one for laser irradiation, as shown in Figure 6-6.
(a) Physical view of the flow cell assembly
(b) Operation principle of the flow cell
6-4
Optical System
Figure 6-6 Flow cell assembly
Both the removal of internal parts of the flow cell assembly and the removal of the assembly
from the substrate are forbidden. Generally, if the flow cell assembly is determined to be faulty, the
whole optical system shall be replaced.
6.2.4 Optical Substrate Assembly
The substrate assembly provides support, fixation and shock absorption for the optical system, as
shown in Figure 6-7. The screws in the substrate assembly is not removable. If loose screws result in
abnormal condition of the optical system, please replace the whole optical system.
Figure 6-7 Optical substrate assembly
6.2.5 Preamplifier and Shield Shell
The preamplifier and shield shell are used for pre-amplifying the optical system signals and
shielding signal noise. The preamplifier is connected by the preamplifier shield shell with the optical
system shield shell. If the preamplifier is determined to be faulty, it is possible to remove and replace
it from the optical system, as shown in Figure 6-8.
6-5
Optical System
Figure 6-8 Preamplifier and shield shell
6.2.6 Shield Shell Assembly
The shield shell is used for isolating the optical system from the outside to avoid interference of
dust, stray light and electromagnetic noise, and for connecting the optical system to the analyzer, as
shown in Figure 6-9.
Figure 6-9 Shield shell assembly
6.2.7 Scattering Detection Assembly
As the detection unit of the optical system, the scattering detection assembly consists of
medium/small angle PD and large angle PD and corresponding medium/small angle aperture and
6-6
Optical System
large angle aperture and support structure. The 3D model and physical view are shown in Figure 6-10.
The scattering assembly is a whole unit and can not be removed from the optical substrate, although
the large angle PD assembly is removable. When the scattering detection assembly is determined to
be faulty, it is necessary to replace the whole optical system.
Figure 6-10 Scattering detection assembly
6.2.8 Laser Control Board
The laser control board is used for providing stable driving current for the laser to keep a full and
stable laser output power, as shown in Figure 6-11. If the laser control board is determined to be
faulty, it is possible to remove and replace it from the optical system.
Figure 6-11 Las
6.3. Determinating the Optical System Status
When abnormal sample scattergram causes failed or incorrect categorization, and the reagent and
fluidic connections are determined to be normal, it will be necessary to check the optical system
status. In this section, the optical system status is tested with standard particles. Prior to the test,
prepare the following items:
Consumables: 4k-07 Std. part. (7µm) and 1.5 mL centrifuge tube, slobe cleanser.
The detailed procedure is as follows.
1.
First, add 0.5 mL of deionized water to a 1.5mL centrifuge tube. Shake the 7 μm std.
6-7
Optical System
part. bottle until the solution is well mixed, then add 3 drops of the solution in to the
1.5mL centrifuge tube. Cap the tube and shake it until this solution become well
mixed, as shown in Figure 6-12.
Figure 6-12 Preparation of the 7µm std. part. solution
2.
3.
Select the Maintenance menu and enter the “Optical” screen. Perform counting with
the prepared std. part. solution. After the counting is completed, the result will be
automatically displayed in the screen, as shown in Figure 6-13.
Determine the optical system status by the parameter Particle 1 according to the
counting result. The optical system is OK if all the following requirements are met:
Parameter
Total
LAS
MAS
WAS
4.
1500~3000
CG Position
CV
38~45
≤6.50
100~158
≤3.00
100~200
≤8.00
Generally, if the parameters fail to meet the requirements, it may be caused by a dirty
flow cell. The built-in maintenance program can be used to solve this problem. The
method is as follows: click the “Maintenance-->Maintenance” menu and select “Flow
Cell Cleaning” in the “Cleaning” screen. The machine will automatically complete the
cleaning operation (in about 1 minute).
After the maintenance, repeat step 2 and 3 and check if the result meets the requirements. If not,
probe cleanser should be used to clean the flow cell: click the “Maintenance-->Maintenance” menu
and select “Overall Soak” in the “Maintenance” screen, and carry out the operation of the program.
After the maintenance above, repeat step 2 and 3 again, and check if the result meets the requirements.
If still not, the manual maintenance is required. For details, please see the next chapter.
6-8
Optical System
Figure 6-13 Optical screen of the stanadard particle
6.4. Maintenance and Replacement of the Optical
System
When the optical system is determined as abnormal according to Section 6.3, and can not be
restored by automatic maintenance, then manual maintenance will be necessary. Furthermore, if the
optical system is determined as normal according to Section 6.3, but the scattergram or categorization
of the blood sample is abnormal, then other problems except the optical system shall be taken into
account.
Before performing maintenance and replacement on the optical system, prepare the following
items:
Tools: A crosshead screwdriver and an allen wrench set.
Consumables: 4k-07 Std. part. (7µm), 1.5 mL centrifuge tube, microfiber clean cloth,
dehydrated alcohol and probe cleanser.
6.4.1 Maintenance of the Optical System
Before maintenance of the optical system, it is necessary to open the top cover of the optical
system shield shell. As shown in Figure 14, use a crosshead screwdriver to remove the locking screw.
Open the top cover carefully to reveal the internal structure of the optical system, as shown in Figure
6-4.
6-9
Optical System
Tap "Status" > "Voltage&Current" in the menu to enter the Voltage&Current screen, the
laser will illuminate automatically.
Figure 6-14 Location of the screws on the shield shell
Precautions before proceeding to the next step are as follows:
Never look directly into the laser with eyes or through an optical instrument;
During test of optical system with the top cover open, please shelter the optical system to
prevent bright environmental light from irradiating the detector inside the optical system.
Generally, the following steps can be followed to determine which part needs to be maintained.
1.
Check if the wires are firmly connected inside the optical system and if the optical path is
blocked by wires.
2.
Check if the output spot is normal. Place a small piece of white paper near the output exit
and observe the light spot. The ideal spot shape is a vertical ellipse which is clipped at both
top and bottom, as shown in the left in Figure 6-15. The actual spot is shown in the right in
Figure 6-15 with a faint halo around it.
6-10
Optical System
Figure 6-15 Light spot at the output exit
Abnormal spots can be different, including dark spot, spot clustered into a dot, spot with dark
lines, spot with a scattered halo, seriously damaged spot, spot with multiple dark dots in the center, as
shown in Figure 6-16.
Figure 6-16 Example of abnormal output spots
Abnormal light spots are usually caused by damage or contamination of the laser control board,
the laser or the lens in the light source assembly.
Missing parts, dark lines or dark dots in the spot are usually caused by contaminated lens in the
light source assembly. A clean cloth dampened with dehydrated alcohol may be used to wipe the lens
gently, spiraling outward from the center. Be careful not to touch the interior of the lens barrel.
If the light spot disappears, darkens or diverges, then the laser control board may be damaged.
After the problem is determined, replace the laser control board (be aware of electrostatic) separately
and adjust the variable resistor on the board. Perform a std. part. test in accordance with 6.3 to ensure
the std. part. indicators to meet the requirements. After replacement, a gain calibration in accordance
with Section 4.6 as well as a calibration in accordance with Section 4.3 shall be performed on the
optical system.
6-11
Optical System
If the light spot disappears, converges, darkens or diverges, and the laser control board is
determined to be in good condition, then the laser could have been burned. Since the light source
assembly can not be replaced separately, it will be necessary to replace the optical system.
3.
Observe the light spots on the surface and inside the flow cell from the following angles, as
shown in Figure 6-17. If the exterior of the flow cell is very bright, then the exterior may be
stained. Use a clean cloth dampened with dehydrated alcohol to wipe the exterior, until the
bright part darkens or disappears, as shown in the right in Figure 6-17. If the interior of the
flow cell is very bright and can not be darkened by the built-in cleaning procedure, then it
will be necessary to manually rinse the inside of the flow cell.
Figure 6-17 Flow cell before and after cleansing
The cleansing procedure is as follows: Prepare a syringe with a 100 mm long Teflon
tube, a 200 mm long Teflon tube and probe cleanser, as shown in Figure 6-18. Shutdown
the analyzer, remove the tubes at the waste outlet and the sheath inlet of the flow cell.
Connect the syringe to the waste outlet. Connect one end of the other Teflon tube to the
sheath inlet, and put another end into the probe cleanser. Draw the probe cleanser with the
syringe until the probe cleanser enters the syringe. At this time, the flow cell will be filled
with the probe cleanser. After soaking for about 10 minutes, use clean water instead of the
cleanser. Draw the syringe forcefully for 2 to 3 times, until the bright spots in the flow cell
darken or disappear.
Connect the optical path and the tubes and verify the connection, and then turn on the
analyzer power.
6-12
Optical System
Figure 6-18 Preparation before cleaning the flow cell interior
4.
Check if the output light is perpendicular to the flow cell. Place an inner hexagon spanner
at the output exit. The output spot and the spot reflected by the flow cell shall appear
simultaneously and strictly coincide with each other, as shown in Figure 6-19.
Figure 6-19 Output spot and reflection spot
After the maintenance is completed, verify if the optical system is working correctly in
accordance with the steps in Section 6.3:
If the std. part. CV meets the requirement, but the CG position is too low or too high,
slightly adjust the variable resistor on the laser control board as shown in Figure 6-11, until
the CG position meets the requirement. After maintenance according to this procedure, a
gain calibration in accordance with Section 4.6 as well as a calibration in accordance with
Section 4.3 shall be performed on the optical system.
If the CG position meets the requirement but the CV doesn't, then the maintenance is not
qualified. Perform another check and maintenance in accordance with this section.
If the CG position and the CV can not meet the requirements after the maintenance, then it
will be necessary to replace the optical system.
6-13
Optical System
6.4.2 Replacement of the Optical System
If the problem is not solved after maintenance, or if the problem is not serviceable, then it is
necessary to replace the optical system with a new one. The replacement procedure is as follows:
1.
Power off the analyzer.
2.
First, gently disconnect the waste outlet tube from the optical system, and connect to a
syringe. Disconnect other tubes, aspirate remaining fluid with the syringe from the system.
Unscrew the screws and disconnect the signal line from the optical system, as shown in
Figure 6-20.
Note: Follow the procedure and prevent the corrosive fluid from falling onto the
frame!
Figure 6-20 Remove the optical system
3.
Remove the optical system carefully and short the tubes of the optical system in accordance
with Figure 6-21.
6-14
Optical System
Figure 6-21 Short the tubes of the optical system together
4.
Install the new optical system into the analyzer. Connect the signal wires, fluidics and
optical path. Turn on the analyzer and verify the status of the new system in accordance
with the steps in Section 6.3.
Note: The fluidic interfaces of the optical system are very fragile. Be careful to avoid
knocking them when connecting the tubes during installation!
5.
Perform a gain calibration in accordance with Section 4.6 as well as a calibration in
accordance with Section 4.3 on the optical system.
6-15
7 Fluidics
7.1. Measurement Flow
The fluidics of the analyzer can be divided into two measurement channels:
WBC&HGB Channel
RBC&PLT Channel
The system flowchart of the WB-CBC+DIFF mode is shown below (DIFF lyse and DIFF
sample configuration & measurement are not available in CBC mode):
Figure 7-1Fluidics flowchart (WB-CD mode)
7-1
Fluidics
The system flowchart of the PD-CBC+DIFF mode is shown below (DIFF lyse and DIFF sample
configuration & measurement are not available in CBC mode):
Figure 7-2Fluidics flowchart (PD-CD mode)
7.1.1 WBC&HGB Channel
DIFF Measurement
Reagents:
7-2
Fluidics
DIFF reagent: used for lysis of red blood cells and specialization of different white blood
cells;
Diluent: background solution used for providing sheath fluid and cleansing
Measurement principle: flow cytometry and laser scatter
Measurement parameters: MONO#, MONO%, LYMPH#, LYMPH %, NEUT#,
NEUT%, EOS#, EOS%
Graphics: 4 Differential scattergram
Dilution ratio: 1:105.8
Counting duration: 9.1 s
Counting flow: 0.008575417ml/s
Counting volume: the flow of the sample stream is constant, which can be
converted to counting volume by controlling the counting duration
Function description: Mix 15 µL of blood sample and 550 µL of diluent in the
WBC bath. After the secondary aspiration, add 1 mL of DIFF lyse. After the reaction
has been on for a certain time, place the prepared sample at the bottom end of the
flow cell. The sample will be wrapped by a sheath stream generated by the large
volume syringe of the syringe linkage and be pushed by the small volume syringe
into the flow cell for the measurement.
WBC Count and BASO Measurement
Reagents:
LH lyse: used for lysing red blood cells and platelets and separating the basophiles by
volume from the other white blood cells
Diluent: used for providing sheath fluid and cleansing
Measurement principle: flow cytometry and laser scatter
Measurement parameters: WBC, BASO# and BASO%
Graphics: WBC histogram
Dilution ratio: 1:123.8*
1
Counting duration: 11.5 s
Counting flow: 0.008575417ml/s
Counting volume: the flow of the sample stream is constant, which can be
converted to counting volume by controlling the counting duration
Function description: After DIFF reaction, add 200 µL of LH reagent to the
sample. After adequate reaction, place the prepared sample at the bottom end of
the flow cell. The sample will be wrapped by a sheath stream generated by the
large volume syringe of the syringe linkage and be pushed by the small volume
syringe into the flow cell for the measurement.
∗1
Dilution ratio refers to the Dilution ratio in the whole blood mode.
7-3
Fluidics
HGB Count
Reagents:
Diluent: used for diluting and cleansing
LH lyse: used for lysing red blood cells and combining hemoglobin
Measurement principle: colorimetric method
Measurement parameters: HGB
Dilution ratio: 1:123.8
Function description: The measurement principle of HGB channel is the colorimetric
method, which obtains HGB concentration by comparing the transmitted light intensity
between background and blood.
7.1.2 RBC/PLT Channel
Reagents:
Diluent: used for diluting, cleansing and equal volume processing conductive environment
and cells
Measurement principle: impedance method
Measurement parameters: RBC and PLT
Graphics: RBC histogram and PLT histogram
Dilution ratio: 1:7533.3
Counting duration: 9 s
Counting pressure: -30kpa
Measurement volume: The measurement volume is controlled by controlling
the vacuum and counting duration. Keep a stable vacuum to ensure a stable flow
out of the aperture. The measurement volume can be calculated by controlling the
counting duration
Function description: Aspirate 21.6 µL of sample (dilution ratio 1:69.75) with
the sample probe from the WBC bath. Move the probe to the RBC bath and mix this
sample with 2.4 mL of diluent to prepare a sample with dilution ratio of 1:7533.3.
After mixing, aspirate the sample with negative pressure in the vacuum chamber
through the aperture into the secondary bath. The cells will be measured while
passing through the aperture.
7.2. Sample Volume
Table 7-1 Sample volume
Item
Whole
Capillary
Prediluted Mode
7-4
Fluidics
Blood
Blood Mode
Mode
CBC+DIFF
15µL
15µL
CBC
11.7µL
11.7µL
Dilution outside the analyzer: 20µL of blood
sample; 480 µL of diluent, 200µL aspirated
Dilution outside the analyzer: 20µL of blood
sample; 480 µL of diluent, 200µL aspirated
7.3. Temperature of Fluidics
Table 7-2 Temperature of Fluidics
preheat bath
Item
Optical system
Diluent
Target
Temperature/
℃
Varies with the
diluent
temperature
/
/
Alarm
Temperature/
℃
＜target
temperature -1.5,
＞target
temperature +3
＞40
＜10, ＞40
7.4. Reagent Consumption Volume
Table 7-3 3107 reagent volume
Diluent
Sample Mode
DIFF Lyse (ml)
LH Lyse (ml) Probe cleanser
(ml)
(ml)
CD
27.5
1
0.2
0
CBC
23.2
0
0.2
0
CD
27.5
1
0.2
0
CBC
23.2
0
0.2
0
CD
27.6
1
0.2
0
CBC
22.9
0
0.2
0
Shutdown
60.9
1
0.2
2
Normal Startup
65
1
0.2
0
Exit Standby 1
3.7
0
0
0
Exit Standby 2
16.9
0
0
0
Exit Standby 3
65
3
0.2
0
Whole Blood Mode
Capillary Blood Mode
Prediluted Mode
7-5
Fluidics
7.5. Introduction to Fluidic Parts
A brief introduction to the fluidic parts and their respective functions is provided in this
section. The symbols mentioned below refer to the symbols in the fluidics diagram.
7.5.1 Mindray Valves
Symbol:
2-way valve
3-way valve
Appearance:
2-way valve
3-way valve
Spring pole
Function:
2-way valve: to build up or cut off a passage. When power off, the passage from
the inlet of the valve to outlet is cut off; when power on, the passage is build up.
3-way valve: to switch among passages. When power off, the public end and the
NO (normally open) end are connected; when power on, the public end and the
N.O.(normally open) end are connected.
Note: the operating voltage of Mindray valves is 12V, and maximal bearable pressure
is 200KPa. The internal movement of the valves is driven by electromagnet and the
restoration is driven by the spring, so it is recommended not put the valves power-on
for too long. When the electromagnet valve is working, the spring pole will lower
down, and it will rise to the initial position when power off. You can touch the spring
pole and feel the descending or ascending, in order to determine whether it is in
action.
7.5.2 2-way Mindray Pressure-proof Valve
Symbol
Same as the 2-way Mindray valve.
Appearance
7-6
Fluidics
Function: Compared with regular 2-way Mindray valve, the 2-way Mindray
pressure-proof valve can endure higher reverse pressure. The operation principle of
the 2-way Mindray pressure-proof valve is the same with that of regular 2-way
Mindray valve.
Note: When replacing the valves, please note the distinction between regular 2-way
valves and pressure-proof valves. SV03 in Liquid Flow Diagram is a 2-way
pressure-proof valve.
7.5.3 LVM Fluidic Valve
Symbol
Same as the Mindray valves.
Appearance
3-way LVM fluidic valve
Function: Same as the Mindray valves. Compared with 2-way Mindray valves, this
valve provides higher pressure resistance and smaller pump volume for preciser flow
control and broader range of temperature and pressure.
Note: the maximal bearable pressure of the LVM fluidic valve is 200KPa, and the CV of
the flow is about 0.03. SV11 in the fluidics diagram is a LVM fluidic valve.
7.5.4 Pinch Valve
Symbol:
7-7
Fluidics
Appearance:
Function: A clamp-on type valve switched by electromagnetic force. Used for
switching the fluid flow.
7.5.5 Liquid Filter
Symbol:
Appearance:
LF
7-8
Fluidics
Function: Used for filtering the impurities in the diluent.
7.5.6 Syringe Linkage
Symbol: SR
Appearance: N/A
Function: Composed of a large volume syringe and a small volume syringe, the syringe
linkage is driven by a motor and a linkage. The parameter and the function of the
syringe linkage are shown in the table below:
Table 7-4 Parameter and function list of the syringe linkage
Name
Specific
Function
ation
Used for quantitative aspiration, distribution
Small
volume
syringe
Full
scale
250µL
and secondary aspiration of blood samples, and for
injecting the sample into the flow cell for
measurement
Used for quantitative addition of diluent to
Large
volume
syringe
Full
scale
10mL
WBC and RBC bath, wipe fluid supply, cleaning
of interior and exterior of sample probe and
reaction bath, forcing the sheath into the flow cell,
cleaning of the flow cell and sample preparation.
7.5.7 Electromagnetic Metering Pump
Symbol
7-9
Fluidics
DP1, DP2
Appearance:
Function:
DP1: 1mL volume. Used for addition of DIFF reagent
DP2: 200µL volume. Used for addition of LH reagent
7.5.8 preheat bath
Symbol
preheat Bath
Appearance:
7-10
Fluidics
Function:
Used for heating DIFF reagents to ensure the temperature of DIFF reaction.
7.5.9 Vacuum Pump
Symbol
Appearance:
Function:
LP: Used for draining the wipe, WBC bath, RBC bath and vacuum chamber,
and for creating vacuum in the vacuum chamber
7-11
Fluidics
7.5.10 Sample Probe
Symbol
Appearance:
Open-vial sample probe
Function: Used for providing a rigid cavity with resistance to blood sample corrosion,
which can sample and dispense the blood as well as aspirate and dispense probe
cleanser.
Note: the sample probe is flat-tipped with a side opening to ensure normal aspiration in case that the
tip touches the bottom of the sample tube.
7.5.11 Probe Wipes
Symbol:
7-12
Fluidics
Appearance:
Open-vial probe wipe
Function: Provide a cavity for cleaning open-vial probe or piercing probe by liquid flow
and collecting waste fluids on the interior or exterior.
7.5.12 Hydraulic pressure sensor
Symbol:
Appearance:
Function: Used for monitoring the fluid pressure. When the pressure is obviously
abnormal or beyond the setting range, the sensor will send an alarm signal.
7-13
Fluidics
7.5.13 1-way Valve
Symbol:
Appearance:
Function: Used for controlling the flow direction of the DIFF tube to prevent reverse
aspiration
7.5.14 Baths
WBC bath: Used for providing a place for WBC sample reactions and
supplying well reacted DIFF and BASO samples, and for HGB measurement.
RBC bath: Composed of primary bath, secondary bath and aperture. Used for
providing a place for RBC sample reactions and for RBC/PLT measurement.
Vacuum chamber: Used for creating and keeping a stable negative pressure
for RBC impedance count.
preheat bath: Used for heating DIFF reagents to ensure the temperature of
DIFF reaction.
WBC isolation chamber: Provide a gas chamber to block interference signals
from outside
RBC isolation chamber: Provide a gas chamber to block interference signals
from outside
7.6. Detailed Introduction of Fluidic Structure
The fluidic structure diagram is shown as follows:
Please refer to Appendix A.
7-14
Fluidics
7.6.1 Sampling and dispensing channel
The structure of sampling and dispensing channel is shown below:
1.
2.
3.
Main function:
Aspirate and dispense samples. Aspirate 15 μL of blood sample by conjunctive use of
small volume syringe of the syringe linkage (SR) and the sample probe (SPB), and
dispense the blood sample.
Clean the interior and exterior of the sample probe. The interior is cleaned by the
collaboration of the large volume syringe of the syringe linkage and SV03 and 11 valves.
The exterior is cleaned by the diluent which is forced by the large volume syringe
through SV08, SV07, secondary RBC bath and SV09 into the wipe, with waste fluid
recovered by probe wipe, vacuum chamber and waste pump.
Aspirate and dispense probe cleanser. During the aspiration, the SV03 is energized.
2mL of probe cleanser will be aspirated by the SR from the SPB and stored mainly in the
cleanser tanks (T7 and T8). During the dispensation, the SPB is transported by the
sampling assembly to the RBC bath and WBC bath, and the SV03 is kept energized. A
certain volume of probe cleanser in the SPB will be dispensed by the SR to the reaction
baths.
7.6.2 WBC&HGB Channel
Part of the fluidic structure is shown as follows:
The blue lines are diluent flows; red lines are DIFF lyse flows; green lines are LH lyse flows;
orange lines are sample flows; purple lines are waste fluid flows. Similarly hereinafter.
7-15
Fluidics
DIFF measurement
As the background solution, the diluent flows through the large volume syring, SV03, SV11,
PV18 into the WBC bath. After the blood sample is dispensed by the sample probe to the WBC
bath, the DIFF lyse is added by the electromagnetic metering pump (DP1) through the SV01 and
the preheat bath. The diluent is forced by the SR through SV06, T13 and T14 into the isolation
chamber 1, generating bubbles which mix the sample fluid in the WBC bath. The sample is then
supplied along the illustrated orange lines to the bottom end of the flow cell. A sheath flow is
generated by the large volume syringe along the illustrated blue lines, and the sample is forced
by the small volume syringe into the flow cell for measurement to obtain the differential results
of the white blood cells. After the measurement, use the sheath to clean the flow cell and the
sample probe. Force the diluent through the SV03, SV11, C11, T35, SV14 and T36 into the
isolation chamber 2 to clean the sample supply tube.
BASO measurement & WBC count
After DIFF sample enters the sample supply tube, the LH lyse is added by the
electromagnetic metering pump DP2 through SV02 to the WBC bath. The diluent is forced by
the SR through SV06, T13 and T14 into the isolation chamber 1, generating bubbles which mix
the sample fluid in the WBC bath. After the DIFF sample measurement is completed and the
sample supply tube is cleaned, the BASO sample is supplied along the illustrated orange lines to
the bottom end of the flow cell. A sheath flow is generated by the large volume syringe along
the illustrated blue lines, and the sample is forced by the small volume syringe into the flow cell
7-16
Fluidics
for measurement to obtain BASO differential result and WBC count. After the measurement, use
the sheath to clean the flow cell and the sample probe. Force the diluent through the SV03,
SV11 and PV18 into the WBC bath to clean the sample supply tube and the WBC bath. During
the cleaning process, part of the diluent flow away through C11, T35, SV14 and T36 to clean the
3-way connector C11. Finally, the WBC bath is drained by SV15, vacuum chamber (VC) and
waste pump.
HGB Count
The measurement principle of HGB channel is the colorimetric method, which obtains HGB
concentration by comparing the transmitted light intensity between background and blood. The
transmitted light intensity of the pure diluent in the WBC bath is measured at the beginning of
the count. The transmitted light intensity of the diluent with blood is measured after the BASO
reaction is completed (before preparation of the BASO sample). The HGB value can be
calculated by comparing the above two values.
7.6.3 RBC/PLT Channel
Part of the fluidic structure is shown as follows:
7-17
Fluidics
The diluent is added by the SR along the blue line (T3, T18, SV05, T21) to the RBC
bath. The diluted blood sample is dispensed by the sample probe to the RBC bath, and
then is mixed by the bubbles generated when waste cleaning fluid (for cleaning the
DIFF sample fluid in the sample supply tube) enters the isolation chamber 2 through
SV14 and T36. The sample is aspirated by the negative pressure in the vacuum
chamber into the secondary bath (orange lines in the illustration). The cells will be
measured while passing through the aperture. The sample volume is calculated from
the count duration. After the measurement, the RBC bath is drained by the waste
pump, the vacuum chamber and the SV13. In order to clean the secondary bath, the
diluent is forced by the syringe through SV07, T51 and T52 into the secondary bath,
then through T43 and SV09 into the probe wipe, and drained by the waste pump.
7.6.4 Precautions for Assembly and Service
Precautions for installation of sampling assembly
No.
Precautions
Strap the tube above the sample probe at the positioning hole to prevent the
tube connector from being stressed by the vertical motion of the sample probe
1
2
Move the sampling assembly horizontally and vertically to ensure that the
sample tube is unobstructed with no folding and interference with the preamplifier,
fluidics separator, right door, motor, valves and tube straps
3
Ensure the sample tube is not squeezed or deformed at the tube straps
4
Ensure there is no folding or interference when the wipe fluid tube is moving
horizontally or vertically in the sampling assembly
5
When the sample probe is in the top position, keep a distance of 5±0.1mm
between the probe tip and the lower end of the wipe
Precautions for installation of reaction bath assembly
No.
Precautions
1
Case T59 and T60 with flexible tubes
2
The waste tube for the reaction bath needs to be wrapped in the vertical direction
to a height above the liquid level with 3mL of liquid in the bath
3
Install the aperture so that the surface with the tapered bore faces the primary bath
Precautions for Servicing the Whole Fluidics
No.
Precautions
1
The tubes shall be unobstructed with no twisting, squeezing, creases and folds
2
Never bend any of the tubes. Pay special attention to the Teflon tubes. If any Teflon
tube is folded, be sure to replace it with a new tube. When a change in direction is
needed, use a large arc for transition.
3
When cutting the tubes, the cutting face shall be perpendicular to the axis of the
tube.
7-18
Fluidics
4
When connecting the adapter with the Teflon tube, make sure the insertion depth is
13~15mm. Keep no clearance between the two connected rigid tubes as far as
possible. Keep the end of the Teflon tubes smooth and unwrinkled.
5
Strap the connections between any 1.0 Teflon tubes and the adapter. Strap at a
position near the end of the Teflon tube, and leave 3-5 mm of Telfon tube aside.
6
When connecting hoses with connectors, valve ports or dosing tubes on the
reaction bath, the end of the hose shall be fully inserted beyond the barbs.
7
It is not necessary to over tighten the straps for fastening the tubes.
8
For T-adapters, the side ports and the middle port shall be treated differently. Please
assemble in strictly accordance with the illustration.
There are strict tolerance requirements for lengths of the following tubes: T4 and
9
T33: ±1 mm, T63: ±2 mm， T7, T8: ±3 mm.
Tolerances for the other tubes: for length less than 50 mm, the tolerance is ±1 mm;
for length between 50 and 400 mm, the tolerance is ±2 mm; for length more than
400 mm, the tolerance is ±5 mm.
10
When connecting or replacing tubes on the analyzer, never use any blades or other
sharp tools.
11
Never scald any hose with hot water.
12
Before or after assembly or maintenance, keep all the hoses, connectors or fluidic
components intact and in good condition without any scratches, deformation or
distortion.
13
Thick 50 tubes can not be used again after disconnected from valve ports or
connectors
14
Cut T75 and case it between T34 and C11
15
Assemble the check valves so that the bigger end is connected to the preheat bath
16
Assemble the filters in the right direction so that the words 43 μm is shown from
top to bottom
7.7. Introduction to Sequences
Taking the WB-CBC+DIFF mode as an example:
7.7.1 Measurement sequence in WB-CBC+DIFF mode
Measurement time in WB-CBC+DIFF mode adds up to 60 s.
The sequence is as follows:
7-19
Fluidics
0~0.9 s
The small volume syringe of the SR syringe linkage aspirate 15 µL of blood
sample from the sample probe
The large volume syringe aspirates 600 µl of diluent from the diluent tank
0.1 s
Measure the HGB blank voltage
0.3~2.8 s The liquid pump (LP) is turned on to drain the WBC bath
1~2.7 s
Aspirate 5360 µl of diluent with the SR (with SV03 and PV18 energized and
SV11 open to T64)
0.1~3.1s
Burn the aperture on the RBC bath
7-20
Fluidics
2.8~3.8 s
The sample probe travels up to the wipe position
4.2~5.5 s
The sampling assembly swings to the position above the WBC bath
2.9~4.2s
Use the SR to clean the interior and exterior of the sample probe, and keep
the LP pumping the waste fluid during the whole process
4.2~5.3 s
The WBC bath is drained for the second time
7-21
Fluidics
5.5~6 s
SR injects 240 μL of background solution to the WBC bath
5.2~6 s
The sample probe travels down into the WBC bath
6.1~6.5 s
The small volume syringe of the SR slowly injects 18 μL of blood through the
interior of the sample probe The large volume syringe injects 720 μl of fluid to clean the inlet of
the RBC bath
6.55~7.4 s
The syringe quickly injects 312 μl of fluid to clean the sample probe
(with SV03 energized)
5.3~7.3 s
The RBC bath is drained
7-22
Fluidics
7.5~8 s
Inject the diluent through SV06 into the isolation chamber 1 to generate
bubbles which mix the sample fluid in the WBC bath
7.4~8.1 s
The sample probe travels up to the wipe position and the LP draws waste
fluid from the wipe
8.1~8.4 s
The interior of the sample probe is cleaned and the LP draws waste fluid
from the wipe (to 9.2 s)
8.5~9.1 s
The syringe aspirates 1580 μl of diluent from the diluent tank
8.8~9.6 s
The sample probe travels down into the WBC bath
8.6~10.6 s
DP1 aspirates the DIFF reagents
7-23
Fluidics
9.6~10.3 s
The small syringe aspirates 12 μl of diluted sample through the probe
from the WBC bath; the large syringe aspirates 480 μl of diluent from the diluent tank
10.3~11.3 s
The sample probe travels up to the wipe position; the exterior of the probe is
cleaned by the wipe; the LP draws waste fluid from the wipe
10.6~11.6 s
Use DP1 to add DIFF reagent to the WBC bath
11.4~12.3 s
Inject the diluent through SV06 into the isolation chamber 1 to generate
bubbles which mix the sample fluid in the WBC bath
11.2~11.9 s
The sampling assembly swings to the position above the RBC bath
9.2~10.1 s
,11.4~12.1 s
The RBC bath is drained.
7-24
Fluidics
12.4~13.3 s
SR injects 1600 μL of background solution through SV05 to the RBC bath.
12.3~13.2 s
The sample probe travels down into the RBC bath
13.4~14.9 s
The sample probe dispenses the blood and clean the interior with 800 μL of
fluid
15.4~16.6 s
The sample probe travels up to the wipe position and the LP draws waste
fluid from the wipe
7-25
Fluidics
SV08
CV1
J29
Preheat
Bath
T26
T72
T54
T70
DIFF Reagent
Bottle
T55
C7
T16
T41
T46
C16
C8
T58
SV10
Transducer
T56
T57
T67
T66
T43
T74
SV09
T44
Waste
Tank
WASTE
C25
C24
T69
LP
T68
SR
15~17.3 s
The syringe aspirates the fluid
17.7~18.8 s
Aspirate 380 μL of DIFF sample fluid from the WBC bath into the sample
supply tube (T63 and T65)
19.2~19.8 s
Aspirate 200 μL of diluent through C11, T35 and SV14 into T63; adjust the
position of the DIFF sample fluid in the sample supply tube
18.1~19.5 s
DP2 aspirates the LH reagent
17~18 s
The sampling assembly swings to the top sampling position
7-26
T73
VC
Diluent Tank
SPB
J1-T4J2
J27-T22-J28
J3-T5
T2
T1
C9
SV15
J5-T7-J6T8
J4-T6
SV03
T39
T42
T10
T53
SV13
T38
T37
T36
T11
C20
C21
SV12
C13
T62
T40
T15
T14
J18-T60J19
C12
T61
C6
Isolation Chamber
2
C5
T52
T33
SV11
C26
T51
C10
J16-T59J17
Isolation Chamber
1
T34
Case
T75
SV14
T13
Hydraulic
Sensor
RBC
C18
C19
T71
T29
J9
C11
J10-T35J11
J12-T45J13
J22-T64-J23-T65J24
T3
T9
SV06
T12
C4
SV16
LH Reagent Bottle
J30-T78J31
T18
WBC
PV18
J20-T63J21
C1
T47
SV07
J25-T23-J26
C2
T50
SV02 DP2
SV04
C3
C15
DP1
Case
T79
Temperature
Sensor
C23
T49
J14-T48-J15-T80-J32
T77
T17
C27
C22
T30
SV01
T32
T20
T24
T21
SV05
T76
DILUENT
C14
T25
J7-T31J8
LF
C17
T19
Fluidics
19.5~20.5 s
Use DP2 to add LH reagent to the WBC bath
19.8~20.5 s
Inject the diluent through SV06 into the isolation chamber 1 to generate
bubbles which mix the sample fluid in the WBC bath
20.5~31.6s
The large syringe injects the diluent through SV04 into the flow cell to form
the sheath
20.5~20.7 s
SV03 ON; the sample in T65 enters the flow cell quickly by the help of the large
syringe
20.7~31.6 s SV03 OFF; the small syringe injects the sample from the supply tube into the flow
cell to form a stable sample stream
22.2~31.3 s DIFF measurement
31.2 s
Measure the HGB voltage
7-27
Fluidics
SV08
J29
J7-T31-J8
SV01
PV18
T72
T54
DIFF Reagent
Bottle
T55
T70
J18-T60-J19
T41
C16
C8
T57
T58
SV10
Transducer
T67
T66
T43
T44
Waste
Tank
WASTE
C25
C24
T69
LP
T68
SR
31.7~33.6 s
The large volume syringe aspirates the diluent from the diluent tank ; the
small syringe aspirates 156.3 μl of sample fluid from the WBC bath
34~35.5 s
Clean the sample supply tube with SV11 and SV14; clean the flow cell with
SV04 and SV16; inject the waste fluid into the isolation chamber 2 to generate bubbles for
mixing the sample in the RBC bath
31.5~39.1 s
Create negative pressure in the vacuum chamber
7-28
T73
VC
SV09
Diluent Tank
SPB
T74
J1-T4-J2
C7
T16
T46
J27-T22-J28
J3-T5
SV03
T1
C1
C9
T42
T10
T53
T39
SV15
J5-T7-J6-T8
J4-T6
T2
C2
C26
SV13
T38
T37
T36
T11
C20
C21
SV12
C13
T62
T40
T15
T14
SV11
Hydraulic
Sensor
T71
C6
T56
C5
C18
C19
T52
T33
C12
T61
SV14
T13
C10
Isolation Chamber
2
T34
Case
T75
J16-T59-J17
Isolation Chamber
1
SV16
C11
J10-T35-J11
T3
T9
J12-T45-J13
J22-T64-J23-T65-J24
J20-T63-J21
C3
RBC
T51
WBC
SV06
LH Reagent Bottle
J9
T29
SV07
J25-T23-J26
T12
T47
SV02 DP2
SV04
C4
T50
J30-T78-J31
T18
Case
T79
Temperature
Sensor
C15
DP1
J14-T48-J15-T80-J32
T77
T17
C27
C23
T49
T26
T32
C22
T30
SV05
Preheat
Bath
T20
T24
T21
T76
DILUENT
C14
T25
CV1
LF
C17
T19
Fluidics
SV08
CV1
T21
J29
SV05
Preheat
Bath
J7-T31J8
C27
T20
C22
C23
T49
T30
SV01
T32
T76
DILUENT
C14
T25
LF
C17
T19
C15
DP1
T50
J14-T48-J15-T80-J32
T47
SV02 DP2
Case
T79
C18
C19
SV07
SV04
WBC
RBC
PV18
J25-T23-J26
J20-T63J21
Temperature
Sensor
C11
T34
Case
T75
C10
T52
T33
C6
C20
C21
SV12
SV16
C12
T61
SV14
C13
T62
SV06
C5
T13
T12
C4
SV13
T14
T37
T38
T39
C9
T36
C3
SV11
C2
Hydraulic
Sensor
C26
T53
SV15
J5-T7-J6T8
T46
J4-T6
C16
C8
T57
C1
C7
T16
T11
Transducer
T58
T67
SV10
SV03
T66
T43
SPB
VC
SV09
WASTE
T44
Waste
Tank
C25
C24
T69
LP
T68
SR
35.8~37.1 s
Aspirate 800 μL of BASO sample fluid from the WBC bath into the sample
supply tube (T63 and T65)
37.4~51.2 s
The large syringe injects the diluent through SV04 into the flow cell to form
the sheath
37.4~37.6s
SV03 ON; the sample in T65 enters the flow cell quickly by the help of the
large syringe
37.6~51.2 s
SV03 OFF; the small syringe injects the sample from the supply tube into the
flow cell to form a stable sample stream
39.6~51.1 s
Perform BASO measurement and WBC count
35 s
The constant current source of RBC bath turns ON
35 s~49 s
SV12 valve ON; the fluid in the primary RBC bath is forced by the vacuum
through the aperture into the secondary bath
39.8~48.8 s
Perform RBC and PLT counts
7-29
Fluidics
51.3~52 s
PV18 stays ON; the sample supply tube and the 3-way connector C11 is
cleaned by SV03, SV11, and SV14 Clean the flow cell and the sample probe by SV04
52.1~53.8 s
The syringe aspirates the fluid
51.1~52.9 s
The RBC bath is drained
7-30
Fluidics
SV08
J29
J7-T31-J8
SV01
T72
T54
Bottle
DIFF Reagent
T70
J18-T60-J19
T41
C16
C8
T58
SV10
Transducer
T56
T57
T67
T66
T43
T74
SV09
Waste
Tank
WASTE
C25
C24
T69
SR
The WBC bath is drained
SV14 ON; C11 is flushed
SV04 ON, the sample probe is flushed
PV18 OFF; the pneumatic pinch tube and the WBC bath are flushed
The WBC bath is drained
7-31
LP
T68
T73
VC
Diluent Tank
SPB
T44
52.9~54.2 s
53.8~54.3 s
53.9~54.2 s
54.1~55.7 s
55.5~56.9 s
T55
C7
T16
T46
J27-T22-J28
J3-T5
J1-T4-J2
C9
T42
T10
T2
T1
SV03
T39
SV15
J4-T6
C1
SV13
T38
T37
T36
T11
C20
C21
SV12
C13
T62
T40
T15
T14
Isolation Chamber
2
C6
C5
J5-T7-J6-T8
T71
T52
T33
SV11
T53
T51
C10
C12
T61
SV14
T13
T12
T34
Case
T75
J16-T59-J17
Isolation Chamber
1
SV16
C11
J10-T35-J11
T3
T9
SV06
J12-T45-J13
J22-T64-J23-T65-J24
J20-T63-J21
C2
RBC
PV18
Temperature
Sensor
LH Reagent Bottle
T29
J9
WBC
C26
C18
C19
SV07
J25-T23-J26
Hydraulic
Sensor
T47
SV02 DP2
SV04
C3
T50
J30-T78-J31
T18
Case
T79
C4
C15
DP1
J14-T48-J15-T80-J32
T77
T17
C27
C23
T49
T26
T32
C22
T30
SV05
Preheat
Bath
T20
T24
T21
T76
DILUENT
C14
T25
CV1
LF
C17
T19
Fluidics
55.8~56.9 s
57~58.9 s
59.1~60 s
59.1~59.4 s
58.3~59.4 s
59.4~60 s
Add 2560 μL of diluent to the RBC bath with syringe
Add 2018 μL of diluent to the WBC bath with syringe
The sample probe travels to the sampling position
Use the small syringe to create a 4 μL isolation bubble in the sample probe
The liquid pump (LP) is turned on to drain the wipe
SV10 ON; the vacuum is released
7.7.2 Measurement sequence in PD-CBC+DIFF mode
Measurement sequence in PD-CBC+DIFF mode is basically the same with the sequence in
WB-CBC+DIFF mode, except that:
1. Because the blood sample has been prediluted outside the analyzer, 200 μL of blood is
aspirated in the PD mode.
7-32
Fluidics
7.7.3 Measurement sequence in CBC mode
Compared with the sequence in CBC+DIFF mode, the measurement sequence in CBC mode
is basically the same except that it does not include the fluidic actions of DIFF and optical
channels. The main differences are as follows:
1. Sample volume. The sample volume is 15 μL in the WB-CBC+DIFF mode, 11.7 μL in the
WB-CBC mode. The sample volume is the same between the PD-CBC+DIFF mode and
PD-CBC mode.
2. There is no actions related to DIFF measurement, including addition of DIFF reagent,
preparation of DIFF samples, DIFF measurement, and cleaning process of the tubes and
the flow cell after the DIFF measurement.
7.7.4 Introduction to the Maintenance Sequences
Probe cleanser maintenance (shutdown sequence)
The locations soaked by the probe cleanser are shown below.
The orange lines are the locations soaked by the probe cleanser, and the blue lines are the
locations passed by the probe cleanser.
An enhanced probe cleanser maintenance sequence will be called every 300 times of
sample measurement. The main difference between the enhanced and the regular probe
cleanser maintenance sequence is the soaking time. The soaking time of enhanced maintenance
is 6.67 minutes longer than regular maintenance.
In the Maintenance screen, the probe cleanser maintenance is defaulted as enhanced probe
cleanser maintenance.
7-33
T24
T72
T71
LH Reagent Bottle
T51
T70
DIFF Reagent
T40
Bottle
T54
J18-T60-J19
Isolation Chamber
1
J16-T59-J17
Isolation Chamber
1
J10-T35-J11
J12-T45-J13
J22-T64-J23-T65-J24
T3
T9
T73
T74
Startup cleaning
The fluidic actions on startup can be divided into three procedures:
1. Initialization of fluidics components: initialization of sampling assembly and syringe
assembly; 1 metering pump action (without consumption of reagents); creating vacuum and
releasing vacuum.
2. Cleanup: including cleaning of all the tubes and components on the analyzer; removing
bubbles from the flow cell; discarding 1 ml of DIFF reagent and 0.2 ml of LH reagent.
3. Background: WB-CBC+DIFF measurement
If the background fails, perform the cleanup again and measure the background.
For startup after an abnormal shutdown, perform the cleanup twice.
Standby
The instrument will enter standby status after idling for 15 to 30 minutes (defaulted as 15
minutes). After entering standby status, operations without fluidic actions can be performed
from the screen.
Exit standby status 1: Standby for less than 1 hour.
Clean the exterior of the sample probe and the WBC bath and rebuild the
7-34
Diluent Tank
T56
T42
J27-T22-J28
J1-T4-J2
J3-T5
T10
T41
T15
T55
J9
T29
J30-T78-J31
T18
T77
T17
T26
Fluidics
Fluidics
isolation bubble without consumption of lyse.
Exit standby status 2: Standby for more than 1 hour and less than 5 hour.
Clean the interior and exterior of the sample probe, the WBC bath, the RBC bath, the
sample supply tube and the flow cell, and rebuild the isolation
bubble without consumption of lyse.
Exit standby status 3: Standby for not less than 5 hour.
The same as cleanup on startup, including cleaning of all the tubes and components on the
analyzer; removing bubbles from the flow cell; discarding 3 ml of DIFF reagent to eliminate the
effects of bubbles in the preheat bath tube; discarding 0.2 ml of LH reagent to eliminate the
effects of crystal and bubbles at the inlet.
7-35
8 Hardware System
8.1. Hardware System Overview
The hardware system consists of not only power board, main control board, indicator board and
analog drive board for touchscreen, but also drivers and components that requires power supply, such
as motors, valves, pumps, sensors, display and input power filter, and connecting wires between
different boards and components.
8.1.1 Functional Block Diagram
The functional block diagram of the hardware system is shown in Figure 8-1.
Data Channel
Sensor
Signal
preprocess
Master Control
Signal
sampling
Signal
preprocess
Channel Control
Sample review
Touch screen
Result display
TFT monitor
Result print
USB printer
Data storage
Data manager
Network
interface
Input module
Bar-code
scanner
Drive/Detect
Control
Valve and
pump control
Motor
control
Optical
switch
control
Alarm
control
POWER
supervise
POWER
Start key
Supervisor
system
Indicator
board
State
supervise
Figure 8-1 Functional block diagram of the hardware system
The hardware system consists of five modules, including power supply, data stream channel,
control system, drive components and peripherals. The functions of each modules are as follows:
1.
Power supply: provides all kinds of power for each board, component and devices in the
hardware system;
2.
Data stream channel: for extraction, modulation, amplification, collection and preprocessing
of signals;
3.
Control system: for data collection, data processing, result display and sample storage. The
8-1
Hardware System
control system is also the scheduling and managing center, which controls and responds to
all the peripherals and devices;
4.
Drive/monitor: for controlling valves, pumps and motors, monitoring photocoupler and other
important parameters, collecting measurement data and triggering alarms;
5.
Peripheral interface: includes display/touchscreen, USB ports (for printer, keyboard and
barcode scanner) and Ethernet interface. Peripherals also includes operation indicators and
key inputs.
8.1.2 Electrical Connection Block Diagram
The diagram below is the hardware connection block diagram. For electrical connection diagram
of the optical system, please refer to the optical system hardware section.
Figure 8-2 Electrical connection block diagram of the hardware system
The detailed electrical connection diagram of the hardware system is shown in Appendix C.
8.1.3 System Troubleshooting
Common hardware system failures can be divided into board failures, wire failures and
component failures. Generally, the troubleshooting procedures of these failures can be found in the
board troubleshooting section below. However, when the system power supply can not be guaranteed
8-2
Hardware System
(such as failure to power up or immediate system self-protection after power-up), it will be necessary to
start troubleshooting from the system level. Figure 8-3 shown the flowchart for power anomaly check.
Figure 8-4 shows the filter which is located below the rear of the analyzer, used for controlling power
supply and frequency filtering.
Figure 8-3 Abnomal power troubleshooting flowchart
Figure 8-4 Actual picture of the filter
Figure 8-5 shows the troubleshooting flowchart for immediate self-protection after
power-up.
8-3
Hardware System
Figure 8-5 Troubleshooting flowchart for power-up protection
8.2. Digital Control Board
8.2.1 Overview
The digital control board consists mainly of a digital part and an analog part. The analog part
implements mainly the A/D conversion of analog signals into digital signals, such as optical channel
and monitoring voltage. As the essential part of the digital control board and the whole hardware
system, the digital part implements data processing, output, control and communication.
Section 8.2 of this manual is the guideline for service and troubleshooting of the control board.
8.2.2 Components
The block diagram of the control board is shown in Figure 8-6. The control board consists mainly
of digital circuit, plus part of the ADC circuit which implements the A/D conversion. The digital circuit
module implements data processing, result saving and output. As the essential part of the digital control
board and the whole hardware system, the digital circuit is also responsible for control and
communication. The ADC circuit is mainly responsible for digitizing WBC, RBC/PLT, optical signals
and all kinds of analog monitoring signals with an A/D converter.
The control part of the control board employs the “CPU+FPGA” structure to achieve the
following main functions:
A/D conversion
Data processing
Peripheral interface implementation
Control interface extension
8-4
Hardware System
Figure 8-6 Block diagram of the control board
Description
A/D conversion
Converts the analog signals to digital signals which can be processed by FPGA or CPU.
Data processing
The digital signals obtained by FPGA from A/D samples are digitally filtered to save
the particle parameters. The data then is transmitted to CPU by interruption or other methods
for further processing. After processing, the results will be shown on the LCD.
Peripheral interface implementation
The CPU module provides a platform for system software, interfaces for peripherals
including indicator board, LCD, Ethernet, USB printer, USB barcode scanner, keyboard and
USB drive, etc., the JTAG interface for online programming the FPGA configuration chip,
and the CPU debug interface.
Control interface extension
Provides control logic and interface for LCD, SD card and touchscreen, etc.
8-5
Hardware System
Figure 8-7 Schematic of the digital control board module
Interface definition
The functions of the 13 socket interfaces on the control board is listed in Table 8-1. The locations
of each interface on the board is shown in Figure 8-8.
J78 Indicator
J99 Start KEY
J81 Communication
interface
J68 POWER
J86 supervise
signal interaface
J77 control for
Analog&driver
board and optical
system
J2 USB
J3 USB
J1 Network
J85 Optical
signal input
interface
J16 TFT Monitor
8-6
J4 TFT Monitor
Backlight
J67 Touch
Screen
Hardware System
Figure 8-8 Interface locations of the digital control board
Table 8-1 Function list of the control board interfaces
Interface
Function
PIN Function Description
Description
J1
Ethernet interface
/
Network interface
J2
USB port
/
Connect different
USB peripherals
J3
USB port
/
Connects different
USB peripherals
J4
LCD backlight
PIN1: Backlight power
Drives backlight and
control interface
supply, 12V
control the
PIN3: Backlight power
brightness
supply, GND
PIN5: Enables backlight,
3.3V
PIN6: Drives backlight,
0.7~1.2V
J16
LCD signal
PIN3: Power supply, 3.3V
Provides differential
interface
PIN5: GND
signal for the LCD to
display data
J67
J68
Touchscreen
PIN1: Power supply, 3.3V
Connects the
signal interface
PIN4: GND
touchscreen
Power interface
PIN1: Power supply, 5V,
Supply power for
GND
the boards
PIN2: Power supply, 12V
GND
PIN3: Power supply, 5V
PIN4: Power supply, 12V
J77
J78
Analog board
PIN1: GND
Analog board
control and optical
PIN22: 3.3V
control and optical
SPI interface
PIN24: 5V
SPI interface
Indicator board
PIN1: 5V
Drives indicator and
interface
PIN2: RED_LED
buzzer
PIN4: GREEN_LED
PIN6: YELLOW_LED
8-7
Hardware System
PIN9: GND
J81
Analog board and
/
Analog board and
control board SPI
control board SPI
interface
communication
interface
J85
Optical signal
PIN1: FS Signal
Interface for optical
input interface
PIN3: SS Signal
signal inputs
PIN5: SF Signal
amplified by the
PIN7: WBC Signal
analog board
PIN9: RBC Signal
PIN11: 5V
PIN12: 5V
PIN13: GND
PIN14: GND
J86
Analog board
PIN13: 5V
Provides supervisory
supervisory signal
PIN20: GND
signals for the
interface
J99
control board
Aspirate key
PIN2: GND
Aspirate key switch
switch interface
interface
8.2.3 Adjustment and Tests
All the adjustable parameters of this board are adjusted by commands. To adjust the parameters,
perform parameter adjustment in the software interface.
Function definition of LED indicators
Functions of LED indicators on the control board are shown in Table 8-2:
Table 8-2 Function definition of LED indicators on the control board
Indicator
D6
Function
LED OFF Diagnosis
Network interface indicator
Network disconnected, network cable
which flashes after
failure, control board failure
connected with network
cable and PC
D7
Network interface indicator
Network disconnected, network cable
which is illuminated after
failure, control board failure
connected with network
cable and PC
8-8
Hardware System
D40
D44
USB related signals.
USB peripheral failure, control board
Illuminated after power-up.
failure
power chip indication signal.
Power board failure, control board
Illuminated after power-up.
failure
Function definition of test points
The function of test points are defined in Table 8-3. The zone code of test points are shown in
Figure 8-7.
In order to exclude problems caused by shorted peripherals, remove all the wires except the power
cable before diagnosing a power supply problem.
Table 8-3 Function definition of test points on the control board
No.
1
Test
Point
TP1
Description
Zone
VDD18 1.8 V
Zone 6
voltage monitoring
Diagnosis
The voltage is not 1.8V: control board failure
or power board failure
point
2
TP2
VDD 3.3V voltage
Zone 6
monitoring point
3
TP3
VDD12 1.2 V
The voltage is not 3.3V: control board failure
or power board failure
Zone 6
voltage monitoring
The voltage is not 1.2V: control board failure
or power board failure
point
4
TP4
Backlight enable
Zone 5
The voltage is not 3.3V: control board failure
Zone 5
The voltage is not 0.7~1.2V: control board
signal
5
TP5
Backlight PWM
signal
6
TP6
Backlight GND
failure
Zone 5
/
signal
8
TP9
Analog ground
Zone 1
/
9
TP10
ADC U79 SPI
Zone 1
/
Zone 1
/
Zone 1
The voltage is not 2.5V: control board failure
clock signal
10
TP11
ADC U78 SPI
clock signal
11
TP12
ADC U79 2.5V
reference voltage
or connection failure between control board
J86 and analog drive board
12
TP13
ADC U78 2.5V
Zone 1
8-9
The voltage is not 2.5V: control board failure
Hardware System
reference voltage
or connection failure between control board
J86 and analog drive board
13
TP14
FS analog signal
Zone 7
/
14
TP15
FS analog GND
Zone 7
/
15
TP16
FS AD conversion
Zone 7
/
clock
16
TP17
SF analog signal
Zone 7
/
17
TP18
SF analog GND
Zone 7
/
18
TP19
SF AD conversion
Zone 7
/
clock
19
TP20
SS analog signal
Zone 7
/
20
TP21
SS analog GND
Zone 7
/
21
TP22
SS AD conversion
Zone 7
/
Zone 7
/
clock
22
TP23
RBC AGND
23
TP24
RBC analog signal
Zone 7
/
24
TP25
RBC AD analog
Zone 7
/
signal
25
TP26
RBC VREF
Zone 7
/
reference voltage
26
TP27
WBC VREF
Zone 7
/
reference voltage
27
TP28
WBC AGND
Zone 7
/
28
TP29
WBC analog
Zone 7
/
Zone 7
/
Zone 5
/
Zone 5
/
signal
29
TP30
WBC AD
conversion clock
31
TP32
Touchscreen
interruption signal
32
TP33
Touchscreen reset
signal
33
TP34
Digital ground
Zone 5
/
34
TP35
VDD25 2.5 V
Zone 6
The voltage is not 2.5V: control board failure
voltage monitoring
or power board failure
point
8-10
Hardware System
35
TP36
5 V voltage
Zone 6
monitoring point
36
TP37
12V voltage
The voltage is not 5V: control board failure or
power board failure
Zone 8
monitoring point
The voltage is not 12V: control board failure
or power board failure (the ground connection
of this voltage is J68_PIN2 instead of GND)
37
TP44
DDR2 0.9V
Zone 6
voltage monitoring
The voltage is not 0.9V: control board failure
or power board failure
point
38
TP45
DDR2 0.9V
Zone 6
reference voltage
The voltage is not 0.9V: control board failure
or power board failure
monitoring point
39
TP38,
Digital ground
Zone 6
/
3.3 V voltage
Zone 5
The voltage is not 3.3V: control board failure
TP39,
TP40,
TP41,
TP42,
TP43,
TP46,
TP47
40
TP48
monitoring point
or power board failure
8.2.4 Troubleshooting
Table 8-4 lists common symptoms and relative corrections for the control board only from the
hardware side, not including symptoms caused by software. However, many problems will need to be
tested by software.
Before troubleshooting problems related to the control board, perform the following checks:
1.
whether there is any loose connecting wire or unreliable connection on the control board;
2.
whether the bit numbers on the wires are matching the bit numbers on the control board
sockets; whether there is any broken or damaged wire;
3.
whether the input power of board socket J68 is working properly (measured with a
multimeter, the voltage between PIN1 and PIN3 shall be 5V, and the voltage between PIN2
and PIN4 shall be 12V);
4.
Verify if the indicator on the data board is normal according to Table 8-2.
After the wire connections, input power and indicators are verified to be normal, troubleshoot the
problem in accordance with Table 8-4.
8-11
Hardware System
Table 8-4 Troubleshoot the control board
No.
1
Symptom
Evidence
LCD black
1. Check if the connecting wires between the
screen
Solution
Reconnect the
control board and the backlight interface and
connecting wires
LCD is reliable. If the problem is solved after
from the control
reconnection and power-up, then the problem is
board to the backlight
caused by unreliable wire connection. Otherwise,
and LCD
proceed to the next step.
2. Replace the connecting wires from the
Replace the
control board to the backlight and LCD. If the
connecting wires
problem is solved, then the problem is caused by
from the control
connection failure. Otherwise, proceed to the next
board to the backlight
step.
and LCD
3. Measure the voltage between PIN1 and
PIN3 at J4 with a multimeter. If the measured
Replace the control
board
value is not between 11.50~12.50, then the
problem is caused by backlight power failure.
Otherwise, proceed to the next step.
4. Measure the voltage between TP5 and
TP6 with a multimeter. If the measured value is
not between 0.7~1.1V, then the problem is caused
by backlight brightness control failure.
Otherwise, proceed to the next step.
5. Measure the voltage between TP4 and
TP6 with a multimeter. If the measured value is
not between 3.10~3.50V, then the problem is
caused by backlight enable control failure.
6. Replace the LCD. If the problem is
solved, then the problem is caused by LCD
Replace the
LCD
component failure. Otherwise, proceed to the next
step.
2
LCD
1. Reconnect the connecting wires from the
Reconnect the
display
control board to the backlight and LCD. If the
connecting wires
flickers
problem is solved after reconnection and
from the control
power-up, then the problem is caused by
board to the backlight
8-12
Hardware System
unreliable wire connection. Otherwise, proceed to
and LCD
the next step.
2. Replace the connecting wires from the
Replace the
control board to the backlight and LCD. If the
connecting wires
problem is solved, then the problem is caused by
from the control
connection failure. Otherwise, proceed to the next
board to the backlight
step.
and LCD
3. Replace the control board. If the problem
is solved, then the problem is caused by
Replace the
control board
differential conversion chip (U22) or AM1808
board failure. Otherwise, proceed to the next step.
4. Replace the LCD (LCD component). If
the problem is solved, then the problem is caused
Replace the
screen assembly
by LCD component failure.
3
LCD
1. Reconnect the connecting wires from the
Reconnect the
displays
control board to the LCD. If the problem is
connecting wires
strange
solved after reconnection and power-up, then the
from the control
patterns
problem is caused by unreliable wire connection.
board to the LCD
Otherwise, proceed to the next step.
2. Replace the connecting wires from the
Replace the
control board to the LCD. If the problem is
connecting wires
solved, then the problem is caused by connection
from the control
failure. Otherwise, proceed to the next step.
board to the LCD
4. Replace the control board. If the problem
is solved, then the problem is caused by control
Replace the
control board
board failure. Otherwise, proceed to the next step.
5. Replace the LCD (LCD component). If
the problem is solved, then the problem is caused
Replace the
screen assembly
by LCD component failure.
4
Failed
1. Check if the configured IP of the PC is
Set the IP
network
within the same network segment with the control
address to 10.0.0.3
connection
board (10.0.0.X). If not, set the IP to 10.0.0.3. If
the problem is solved, then the problem is caused
by incorrect IP address. Otherwise, proceed to the
next step.
8-13
Hardware System
2. D6 and D7 are not illuminated after
Reconnect or
power-up and connected with PC, and there is
replace the network
poor contact or bad network cable. Otherwise,
cable
proceed to the next step.
3. D6 and D7 are not illuminated, but the
network cable contact is normal.
5
USB port
1. If D40 is not illuminated after power-up,
is not
then the USB HUB chip (U56) fails. Otherwise,
working
proceed to the next step.
properly
2. If D40 is illuminated after power-up,
replace the USB peripherals (USB mouse, USB
Replace the
control board
Replace the
control board
Replace the USB
peripherals
keyboard or USB drive). If the problem is solved,
then the problem is caused by USB peripheral
failure. Otherwise, proceed to the next step.
3. After all these steps, if the problem still
exists, replace the digital control board.
6
Clock
1. Turn off the power and measure the
reset at
voltage between the two end of Battery clip B1
every
with a multimeter with the battery in place. If the
startup
measured value is less than 1.8V, then the
Replace the
control board
Replace the
button battery
problem is caused by a low battery. Otherwise,
proceed to the next step.
2. Measure the voltage of pin 1 or pin 4 at
X10 with a multimeter. If the measured value is
0V, then the problem is caused by crystal
resonator X10.
7
No
1 Check if the connecting wire of the
response
aspirate key is loose or broken. If so, reconnect or
when
replace the wire.
pressing the
2. If step 1 does not solve the problem,
aspirate key
remove the aspirate key switch plate to check if
there is fluid inside. If so, clean the fluid and
reinstall the switch plate.
8-14
Replace the
control board
Hardware System
8.3. Analog Drive Board
8.3.1 Overview
The functions of analog drive board includes measuring and amplifying the signals from the RBC
channel, the HGB channel and the optical channel, and outputting them to external boards; responding
commands of the control board; controlling mechanical components (such as motor drive assembly)
and fluidics components (such as valves and pumps); detecting component position when controlling
mechanical components (through the photocoupler); detecting pressure when controlling fluidics
components (such as pumps); reporting necessary information (temperature, voltage, etc.) to the control
board.
8.3.2 Components
The analog drive board can be divided by modules into four modules: power module, master
control module, detection module and power driving module. The modules are shown in Figure 8-9.
Analog part
Analog
power
Temp sensor and
optical signal
amplifier
1
2
Digital part
Liquid
pressure
sensor
Pump and Valve
Driver
4
3
Motor Driver
5
7
RBC
11
12
9
HGB
Signal
preprocess
8
Supervise
signal
6
Communication
Module
13
Master
Control
Figure 8-9 Module division of the analog drive board
The analog part modulates and amplifies the WBC, RBC/PLT and HGB signals so that the signals
are basically real and ready for A/D conversion before entering A/D. The A/D module is the interface
between the analog circuit and the digital circuit for sampling the above-mentioned sensor signals and
other supervisory signals and converting analog signals to digital signals for digital circuit.
8-15
For Debug
Air pressure
sensor
10
Hardware System
1. The power supplies power for all the electronic devices on the analog drive board, including
both input power from the analog drive board, and the power converted by the power chip;
2. The master control module is responsible for communicating with the control board and task
scheduling;
3. The detection module is responsible for collecting information such as temperature, pressure,
voltage and fluid in the tubes;
4. The power driving module is responsible for driving the power components such as motor,
heater, fan, valves and pumps;
5. The RBC detection circuit provides the RBC module with constant current source and functions
such like RBC signal modulation;
6. Provides functions such like modulation of optical signals and HGB signals.
Figure 8-10 Location of connectors on the analog drive board
Table 8-5 Function list of the analog drive board interfaces
Interface
J1
Function
Float sensor
PIN
Description
PIN2: GND
Detect float status
Analog drive board
PIN1: Optical
Outputs board status
status signal output
temperature
information to the
PIN2: Aperture
control board
interface
J2
supervisory voltage
8-16
Hardware System
PIN3: Gas pressure
PIN4: AGND
PIN5: Fluid pressure
PIN6: -12V supervise
PIN7: Constant current
source supervise
PIN8: Power 24V
supervise
PIN9: Laser current
supervise
PIN10: Power 12V
supervise
PIN11: Analog 12V
supervise
PIN13: Analog 5V
output
PIN20: AGND
J3
Optical board
control signal
PIN22: 3.3V, provided
by the control board
Controls the optical
boards. This signal is
PIN24: 5V provided by
only buffered by the
the control board to DPM
analog drive board.
PIN23: GND
J4
Optical analog
signal output
PIN1: FS output
Optical signal
PIN3: SS output
output modulated by
PIN5: SF output
the analog drive
PIN9: RBC output
board
PIN14: AGND
J5
HGB bath
interface
PIN1: AGND
PIN2: HGB LED+
PIN4: HGB current
input
PIN5: HGB LED-
J6
RBC bath
interface
PIN1: Aperture
electrode B
PIN2: Aperture
8-17
HGB bath interface
Hardware System
electrode A
PIN3: AGND
J8
Analog power
PIN1: AC120_A
interface
PIN2: AC120_B
Supply analog
power for the boards
PIN4: Analog 12V
PIN5: Analog -12V
PIN3: AGND
PIN6: AGND
J9
Optical system
PIN3: FS input
Optical board
signal input and
PIN7: SS input
analog signal output
control output
PIN11: SF input
and control signal
PIN15: Laser current
input
output
PIN18: Laser control
PIN16: AGND
J10
Temperature sensor
/
3-way temperature
interface
J11
Hydraulic sensor
sensor interface
/
Hydraulic sensor
interface
J12
J13
Photocoupler
interface
/
Photocoupler
interface for
interface for
sampling assembly
sampling assembly
Photocoupler
/
Photocoupler
interface for
interface for syringe
syringe assembly
assembly
J14
Valve 13-18 driver
/
Drives valve 13-18
J15
Valve 1-12 driver
/
Drives valve 1-12
Fan interface
/
Fan interface
Waste pump
/
Waste pump
J17, J18,
J19
J20
interface
interface
J21
Heater interface
/
Heater interface
J22
Power interface for
PIN1: Power 12V
Digital power
digital part of the
PIN3: Power 5V
interface
analog board
PIN5: Power 24V
8-18
Hardware System
PIN2: GND
J23
Motor driver
/
Motor driver
interface
J24
interface
Motor driver
/
Motor driver
interface
J31
interface
Controls analog
/
Controls analog
drive board DPM
drive board DPM by
by the control
the control board and
board and other
other preserved
preserved
interfaces
interfaces
8.3.3 Sockets and Indicators
Function definition of LED indicators
Functions of LED indicators on the analog drive board are shown in Table 8-6:
Table 8-6 Function definition of LED indicators on the analog drive board
Indicator
D102
Function
Heater status
indicator
D104-D109
Diagnosis
LED OFF indicates the heater is not
working
Motor status indicator
LED OFF indicates the motor is not
working
D112
MCU status indicator
LED OFF indicates MCU is not
working. May be caused by analog drive
board failure
D115-D117
FPGA controlled
LED
LED OFF indicates FPGA is not
working. May be caused by analog drive
board failure
D118
D119
Digital 24 V power
LED OFF indicates power failure.
indicator
Check the power board and the analog
Digital 12V power
drive board
indicator
D120
Digital 5V power
indicator
D121
Digital 3.3V power
indicator
8-19
Hardware System
D122
Analog 12V power
indicator
D123
Analog -12V power
indicator
D124
Analog 5V power
indicator
D125
Analog -5V power
indicator
Function definition of test points
Functions of test points on the analog drive board are shown in Table 8-7. The zone codes are
shown in Figure 8-8.
Table 8-7 Function definition of test points on the analog drive board
Test
Point
TP19
Description
Analog 12V
voltage AD
TP21
Diagnosis
Zone
If the voltage is not 2.28V, then the
Zone 8
problem may be caused by power failure or
monitoring point
analog drive board failure
FS baseline
/
voltage AD
Zone 8
monitoring point
TP22
Analog -12V
voltage AD
TP23
If the voltage is not 2.82V, then the
Zone 8
problem may be a power failure or analog
monitoring point
drive board failure
56V voltage
If the voltage is not between 1.25~1.5V,
monitoring point
Zone 8
then the problem may be a power failure or
analog drive board failure
TP26
Hydraulic sensor
output voltage
TP29
Pressure sensor
2.5V voltage
TP30
Zone 3
/
If the voltage is not 2.5V, then the problem
Zone 10
may be a power failure or analog drive
monitoring point
board failure
Pressure sensor
/
output voltage
Zone 12
monitoring point
TP39,
Motor lock
Zone 5
/
8-20
Hardware System
TP40,
voltage
TP49,
TP58
TP59
Reset chip
Above
Low signal indicates the chip is in reset
output signal
Zone 7
status
TP68
Digital ground
Zone 6
/
TP43,
Motor pulse
TP44,
output
TP51
monitoring point
TP69
D115 signal
monitoring point
TP86
Zone 5
Zone 7
Digital 1.2V
power
TP87
/
If the voltage is not 1.2V, then the problem
Zone 6
board failure
Digital 2.5V
If the voltage is not 2.5V, then the problem
Zone 13
monitoring point
Digital 3.3V
power
monitoring point
TP89
Digital 12V
power
monitoring point
TP90
Digital 24V
power
monitoring point
TP91
may be a power failure or analog drive
board failure
Right to
Zone 11
Right to
Zone 4
Right to
Zone 4
Digital 5V
power
TP92
may be a power failure or analog drive
monitoring point
power
TP88
/
If the voltage is not 3.3V, then the problem
may be a power failure or analog drive
board failure
If the voltage is not 12V, then the problem
may be a power failure or analog drive
board failure
If the voltage is not 24V, then the problem
may be a power failure or analog drive
board failure
If the voltage is not 5V, then the problem
Zone 5
may be a power failure or analog drive
monitoring point
board failure
AD U41
/
conversion chip
voltage
Zone 3
monitoring point
TP112
HGB analog
voltage output
Zone 9
/
8-21
Hardware System
TP113
HGB assembly
current to
voltage
/
Zone 9
monitoring point
TP115
HGB LED 2.5V
monitoring point
If the voltage is not 2.5V, then the problem
Zone 9
may be a power failure or analog drive
board failure
TP116
Burning voltage
switch
TP117
Constant current
source switch
TP119,
Laser control
TP120
switch
TP121
RBC relay
control signal
TP122
Zone 12
Zone 12
Zone 12
Analog 12V
voltage
TP123
Zone 12
/
/
/
If the voltage is not 12V, then the problem
Zone 1
may be a power failure or analog drive
monitoring point
board failure
AVDD analog
If the voltage is not 5V, then the problem
5V voltage
Zone 1
monitoring point
TP111,
/
Analog GND
may be a power failure or analog drive
board failure
Zone 11
TP124,
shield is
TP125
the analog
/
ground
TP126
Analog -12V
voltage
TP127
If the voltage is not -12V, then the problem
Zone 1
monitoring point
board failure
AVEE analog
If the voltage is not -5V, then the problem
-5V voltage
Zone 1
monitoring point
TP128
may be a power failure or analog drive
NE555 U75
pulse output
may be a power failure or analog drive
board failure
Zone 1
/
Key definitions
Functions of keys on the analog drive board are shown in Table 8-9:
8-22
Hardware System
Table 8-8 Function definition of keys on the analog drive board
Bit No.
Function
S2
Manually reset the
system
8.3.4 Troubleshooting
1) Validation before troubleshooting
Before troubleshooting driver related problems, check whether there is any loose connecting wire
or unreliable connection on the analog drive board, and whether the bit numbers on the wires are
matching the bit numbers on the analog drive board sockets; whether there is any broken or damaged
wire.
When analog drive board failure is suspected, verify whether the indicators on the analog board
are normal in accordance with the table below.
After all the indicators is verified to be normal, verify the MCU and FPGA from the software
interface (click Menu → click “Status” →“Version information”). If all the versions are correct,
troubleshoot the problem according to the problem types listed in (2).
2) Troubleshooting
Initially locate the problem by the alarm indication, then troubleshoot the problem according to
the type as shown in Table 6-7.
Table 8-11 Troubleshooting and solutions
No.
Problem Type
Description
Troubleshooting and solutions
1
Motor and
This problem
1. Check if the board power supply is working
photocoupler
type includes
properly;
but is not
2. Check if the connection between the motor of
limited to:
corresponding channel and the photocoupler is
a. the motor
reliable, if the connectors on both ends are connected
does not work;
properly, if the marks on the photocoupler and motor
b. the motor
connecting wires match their respective locations,
works, but
and if there is any broken or damaged wire;
motor failure or
3. After verifying 1 and 2, try correcting the problem
photocoupler
by performing Remove Error or Self-Test from the
failure is
software screen;
reported
4. Check if the photocoupler surface of the
corresponding channel is contaminated by dust or
fluids. If so, clean and reinstall the photocoupler. If
8-23
Hardware System
the problem is not solved, replace this photocoupler;
5. If the problem still exits after replacing the
photocoupler, replace the analog drive board;
6. If the problem persists, replace the corresponding
channel motor;
7. If the problem persists, then the problem may be
caused by mechanical component failure (such like
too much friction), please troubleshoot this problem
as a mechanical problem.
Abnormal
1. Check if the corresponding channel motor
motor noise
connecting wire is loose, broken or damaged. If so,
please reconnect or replace the wire with the power
off;
2. Check if any fastening screw of the mechanical
component is loose. If so, please tighten this screw;
3. If both 1 and 2 can be excluded, the problem may
be caused by analog drive board failure. Please
replace the analog drive board;
4. If the problem persists, it will be necessary to
replace the motor assembly.
2
Valves
The valve is not
1. From the Valve Self-test screen, check if the valve
working
is opening and closing correctly (a clap will be heard
properly
on normal open/close of the valve). If so, then the
problem is not in the valve drive. Please search for
the cause in the fluidics;
2. If the valve is not opening and closing correctly,
please check if there is any loose or broken wires or
unreliable connection. If so, please reconnect or
replace the connecting wire;
3. If the problem persists, use wires of other valves to
connect this valve, and check if the problem is in the
valve start circuit or in the valve from the Valve
Self-test screen (for example, if valve 2 is suspected,
use wires of valve 3 to connect valve 2; open and
close valve from the Valve Self-test screen; if the
8-24
Hardware System
valve is opening and closing correctly, then the
analog drive board is damaged and needs to be
replaced; if the valve is not opening and closing
correctly, then the valve 2 is damaged and needs to be
replaced).
3
Pumps
a. abnormal
1. Check if the pump is able to work properly from
pressure
the Pump Seft-test screen. If it is, search for the
b. the pump
problem in the gas circuit;
does not work
2. If the pump is not opening and closing correctly,
please check if there is any loose or broken wires or
unreliable connection. If so, please reconnect or
replace the connecting wire;
3. If the problem persists, replace the analog drive
board;
4. If the problem persists, it will be necessary to
replace the corresponding pump.
4
Communication
Communication
1. Check if the control board indicator and the analog
failure reported
board indicator are normal. If not, then replace
corresponding board(s);
2. Check if the connecting wire between the control
board and the analog board is loose. If so, then
reconnect the wire;
3. If the problem persists, replace the connecting wire
between the control board and the analog board;
4. If the problem persists, replace the analog drive
board and the control board one after another. Most
problems will be solved in this way.
5
Waste sensor
False alarm of
1. Check if the connecting wire on the waste
waste status
connector is loose, wet or broken. If so, please
disconnect and reconnect the connector, or reconnect
the connector after cleaning the fluid, or replace the
connecting wire.
2. If not, replace the waste cap assembly.
8-25
Hardware System
8.4. Power Board
8.4.1 Overview
The power board provides the analyzer with 6 reliable power outputs, including D5V, A+12V,
A-12V, AC120V and P24V.
Interface definition
There are 6 interfaces for external connections on the power board. 4 interfaces are in socket form,
namely J1, J2, J3 and J4; the AC input wire L and N are led from the board side to the plug for external
connection; the small inverter board is directly connected to the power board by the interface socket.
The locations of each interface on the board are shown below:
A small inverter board
Figure 8-11 Schematic diagram of the power board
The functions of each interface are listed below:
Table 8-12 AC input wires
PIN
Definition
L
Connected to utility live wire
N
Connected to utility neutral wire
Table 8-13 Output socket connectors
Name
D5V
Socket No.
J1
Description
PIN1: GND
PIN2, PIN3: 5V
P12V, P24V
J2
PIN1: GND
PIN2: 12V
8-26
Hardware System
PIN3: 24V
A+12V, A-12V
J3
PIN1, PIN4: GND
PIN2, PIN3, PIN5: -12V
PIN6: 12V
AC120V
J4
AC
8.4.2 Replacement and Connection
Purpose: The power board is one of the key components in the analyzer. If any board problem
occurs, it is required to replace or repair the board timely to ensure normal operation of the analyzer.
Please use the following procedure to replace the power board.
Tool: A 107 cross-head screwdriver and a multimeter.
Removal: 1. Shutdown the analyzer and disconnect the AC power cable;
2. Remove the power assembly from the main unit;
3. Open the power housing, remove 4 fastening screws on the power board, and remove
the power board from the housing.
Please wear antistatic gloves when removing the board;
Please make sure the power is off and disconnect the power cable before removing
the boards.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the screws are installed on the board;
2. Connect the power cable and turn on the AC control switch. Now the analyzer will be
initialized, and all the board indicators will be illuminated.
Make sure the power assembly is firmly connected by screws with the main unit.
Only perform these removal procedures after the power board and the surroundings
are cooled down.
8.4.3 Troubleshooting
The troubleshooting procedures for the power board are shown below.
8-27
Hardware System
Figure 8-12 Troubleshooting flowchart of the power board
8.5. Optical Boards
8.5.1 Optical Path and Optical System Workflow
Figure 8-13 shows the schematic diagram of the optical path. The laser is a semiconductor laser
with wavelength of 670 nm. The laser irradiates the cells in the flow cell, which generate scattered light.
This scattered light intensity is measured by photodiode (PD) from various angles. The measurement
signal is then modulated, amplified, and transmitted to analog drive board for further processing. The
scatter measured by the photodiode can be divided into three angles: low angle scatter, medium angle
scatter and high angle scatter. The type and size of the cell can then be determined by the distribution
of scatter intensity on these angles.
8-28
Hardware System
Figure 8-14 Electrical connection diagram of the optical system
Figure 8-14 shows the electrical connection diagram of the optical system. The analog
drive board controls the laser. When the PDs detect the light signal, they will transmit current
signals through the signal wire to the preamplifier. After I/V conversion, modulation and
amplification, the signals will be transmitted through the signal wire to the analog drive board for
further processing. A protective microswitch is connected between the analog drive board and
the laser analog board. When the optical system housing is opened, the microswitch will be
turned off, and the laser will stop working. Therefore, in order to test the laser, press the
microswitch manually to connect the electrical circuit (make sure the wire is connected to the “C”
pin and “ON” pin of the microswitch).
8.5.2 Functions of Optical Boards
SF PD board and FS/SS PD board
The SF PD board and FS/SS PD board are mainly used for photoelectric conversion, which
converts the light irradiated on the PD to electrical current and transmits the current signal to the
preamplifier for further processing.
Preamplifier
Overview
The preamplifier is mainly used for photoelectric conversion and amplification of the three
scatters from the flow cell (forward scatter (FS, also called low angle signal), side medium angle
scatter (SS, also called medium angle signal) and side high angle scatter (SF, also called large angle
signal)). The FS/SS preamplifier and the SF preamplifier share a common PCB, and achieve the
required amplification through welded resistors.
Note: LAS and FS refer to low angle signal, MAS and SS refer to medium angle signal, and WAS
and SF refer to large angle signal, unless otherwise noted. Corresponding abbreviations refer to the
same objects.
8-29
Hardware System
Function
The function of the preamplifier includes power modulation, I/V conversion and signal
modulation.
Power modulation: filters the -12V power supplied by the analog board. The filtered ripples
will be less than 50 mV.
Signal modulation: converts the current signals to voltage signals by I/V. The signals are then
sent to the amplification unit and processed to meet the input requirements of the analog drive
board.
Figure 8-15 Functional block diagram of the preamplifier
Laser Control Board
Overview
The laser control board controls the laser to provide stable and moderate-intensity laser.
Function
The functions of the laser control board include power modulation, laser driving current
monitoring and laser power control.
Power modulation: filters the ±12V power supplied by the analog board. The filtered ripples
will be less than 100mV.
Laser driving current monitoring: measures the electrical current of the laser and send the
results to the analog board for monitoring.
Laser power control: the laser is controlled by a constant power control method. The laser
output power is monitored in real time by a photodetector inside the laser. The result forms a
loop system by negative feedback to achieve constant output power. The power is controlled
within the range of 3 mW~5 mW by adjusting the potentiometer VR1 on the board.
8-30
Hardware System
Figure 8-16 Functional block diagram of the laser control board
Adjusting laser driving current: when a laser overcurrent problem is reported, it is usually
necessary to adjust the adjustable potentiometer VR1 on the laser analog board to adjust the laser
current. when adjusting, observe the laser current changes in the “Voltage and Current” screen in the
Analyzer Status screen. If the current does not change and is not zero when VR1 is being adjusted, then
either the laser analog board or the laser fails. Otherwise the problem may be caused by wire failure,
and it is required to check the wires before adjustment. It is also necessary to adjust the current after
replacing the laser analog board to keep the current within the acceptance. The adjusting method is the
same with above.
8.5.3 Troubleshooting
Table 8-14 Troubleshooting and solutions of optical problems
No.
Symptom
Possible Cause
Solution
1
Laser does
Power/microswitch
1. Check if the connecting wire between the
not work
/laser abnormality
optical system and analog drive board is loose or
broken. If loose, reconnect the wire. If broken,
replace the wire. Otherwise, proceed to the next
step.
2. Check if the microswitch is working
properly. Press the microswitch with power off.
Measure the continuity between “ON” and “C” pin
with a multimeter. If the measured resistance is
greater than 10 ohms, replace the microswitch.
8-31
Hardware System
Otherwise, proceed to the next step.
3. Check the power supply of the optical
system by measuring the analog drive board at J9.1
(AVSS，-12.6~-11.4V), J9.2 (AVCC，
11.4~12.6V), J9.13 (AVSS，-12.6~-11.4V), J9.17
(AVCC，11.4~12.6V), J9.20 (AVDD，
4.75~5.25V), J9.4~J9.16 (GND). If any
measurement is abnormal, check if there is
problem in the power board or the analog drive
board. Otherwise, proceed to the next step.
4. Check if the analog drive board J9.18 is
low. If not, check if the power board and the
analog drive board are working properly.
5. If 1 to 4 are all excluded, then the problem
is in the power board or the analog drive board.
Replace the laser analog board. If the problem
persists, replace the optical system.
2
No signal
output
1. Check if the connecting wire between the
Power/control
signal/other abnormality
optical system and analog drive board is loose or
from FS,
broken. If loose, reconnect the wire. If broken,
SS and SF
replace the wire. Otherwise, proceed to the next
channel
step.
2. Check the power supply of the optical
system by measuring the analog drive board at J9.1
(AVSS，-12.6~-11.4V), J9.2 (AVCC，
11.4~12.6V), J9.13 (AVSS，-12.6~-11.4V), J9.17
(AVCC，11.4~12.6V), J9.20 (AVDD，
4.75~5.25V). If any measurement is abnormal,
check if there is problem in the power board or the
analog drive board. Otherwise, proceed to the next
step.
3. Check if the analog drive board J9.18 is
low. If not, check if the power board and the
analog drive board are working properly.
4. If all the above problems are excluded,
8-32
Hardware System
replace the preamplifier and check if the problem
is solved. If the problem persists, replace the
optical system.
8.6. Touchscreen Drive Board
8.6.1 Overview
The touchscreen drive board provides the interface between the touchscreen and the control board,
converting the user's touch operation to identifiable signals for the control board. The touchscreen drive
board needs to be adjusted before use.
8.6.2 Components
The core of this board is a 4-wire touchscreen control chip TSC2004IRTJR, which communicates
with the control board through the I2C interface to transmit the position information of the touch
points.
Figure 8-17 Touchscreen connection
8.6.3 Troubleshooting
Table 8-15 Troubleshooting and solutions of the touchscreen
No.
1
Symptom
The
Possible Cause
Solution
Touchscreen analog
1. Check if the control board is working
touchscreen
board
properly. If not, please solve the control board
will not
failure/Touchscreen
problem first.
respond to
failure/Loose
touch
connection
2. Check if the connecting wire between the
touchscreen analog board and the control board is
loose or broken. If loose, reconnect the wire. If
broken, replace the wire.
3. If the problems in 1 and 2 are excluded,
then replace the touchscreen analog board and see
if the problem is solved.
8-33
Hardware System
4. After the replacement, if the problem
persists, replace the touchscreen.
2
3
The cursor
Loose connection
1. Check if there is any crack on the
can only move
between the
touchscreen. If there is, it will be necessary to
horizontally or
touchscreen analog
replace the touchscreen.
vertically
board and the
when touching
touchscreen/Broken
the screen
touchscreen
The cursor
can not move
2. Reconnect the wire between the
touchscreen analog board and the touchscreen.
Un-calibrated/Brok
en touchscreen
1. Perform touchscreen calibration in
accordance with the calibration procedure from the
to certain area
screen (if the deviation is too much between the
touch point and the actual cursor position to enter
the touchscreen calibration screen, use a USB
mouse to enter the screen and start the calibration
procedure).
2. If the problem persists, check if the
touchscreen is broken. If so, please replace the
touchscreen.
8.7. Indicator Board
8.7.1 Overview
The indicator board provides the user with sound or light indications to inform the current
analyzer status.
Figure 8-14 Indicator Board
8.7.2 Components
The indicator board consists of red, yellow and green indicators, control circuits and buzzer drive
circuit. The indicator board does not need to be adjusted before use.
8-34
Hardware System
8.7.3 Troubleshooting
Table 8-16 Troubleshooting and solutions of the indicator board
No.
Problem
Description
Troubleshooting and solutions
Dim indicator
There are 7 LEDs for each color. A dim color
Type
1
Indicator
indicated some LED(s) of this color is damaged.
Remove the indicator board and check if all the 7
LEDs are illuminated. If the number of illuminated
LEDs is less than 7, then the board is damaged and
needs to be replaced. IF not, please measure the
voltage between pin 6 and pin7 at J1 of the indicator
board. If the voltage is less than 4.5V, please check if
the 5V power supply of the control board is working
properly.
The indicator is
not illuminated.
1. Check if the control board is working properly.
If not, please solve the control board problem first;
2. Check if the connecting wire of the indicator
board is loose or broken. If loose, reconnect the wire.
If broken, it is necessary to replace all the front panel
signal wires together.
3. If there is no problem in the wires, please
replace the indicator board.
8.8. Motor, Photocoupler and Microswitch
8.8.1 Overview
The motor is used for driving assemblies such like sampling assembly and syringe assembly. The
photocoupler is used for detecting the motor position. The microswitch is used for starting the blood
test process. The schematic diagram of the motor and the photocoupler is shown below.
8-35
Hardware System
8.8.2 Troubleshooting
Table 8-17 Troubleshooting and solutions of motor, photocoupler and microswitch
No.
Problem
Description
Troubleshooting and solutions
Type
1
Motor
The motor does 1、 Check the analog drive board. If there is problem
with the analog drive board, solve it first.
not turn
2、 Check there is any loose or broken wire between the
motor and the analog drive board. If there is, replace
the corresponding wire.
3、 If there is no problem in the wires, replace the
motor.
The motor
won't stop at
the specified
position
2
Photocouple
The motor can
r
not move to
1、 Check the analog drive board. If there is problem
with the analog drive board, solve it first.
2、 Check there is any loose or broken wire between the
photocoupler and the analog drive board. If there is,
replace the corresponding wire.
3. If there is no problem in the wires, replace the
photocoupler.
certain position
8-36
Hardware System
8.9. Liquid sensor board
8.9.1 Overview
The liquid sensor board is used to detect whether there is liquid in the tube, judge whether
there is liquid or not by the refractive ratio for the refractive ratio is not the same when there is
liquid or not.
Figure 8-15 Liquid sensor board
8.9.2 Composition
The main part of the board is photocoupler, there is a LED indicator at the back of the board,
if there is not barrier between the photocoupler transmitting tube and receiving tube, the LED
light is off; if the photocoupler is blocked, the LED light is on. When testing the board function,
use the card to perform photocoupler barrier testing.
8.9.3 Troubleshooting
When testing the reagent, if there is reagent, the board TP4 output is low electrical level
(lower than 0.8V) and the LED light is off, if there is no reagent, output the high electrical level
(higher than 2V) and the LED light is on, when the error occurs, troubleshoot by the LED light
status and whether there is reagent at the testing point.
8-37
9 Heating System
9.1. Heating System Overview
The heating system consists of diluent heating system and optical heating system. The
diluent heating system provides the best temperature range for the RBC bath and the WBC bath.
The optical heating system provides the temperature range for normal operation of the optical
instruments.
The diluent heating system consists of temperature detection assembly and preheat
assembly. A diluent sensor and a preheat sensor work together to detect and control the
heating temperature of the preheat assembly.
The optical heating system consists of power assembly and fan assembly. The optical
system is heated by the heat generated by the power board. When the optical system
temperature exceeds a specified limit, a temperature sensor inside the Optical System will start
the fan assembly, which draws heat from the bottom of the power assembly and controls the
temperature of the Optical System.
The temperature ranges of each detection point are listed below:
Name
Temperature Range °C
Temperature of the diluent
10~40
detection assembly
Temperature of preheat
assembly
Variable. A range can be calculated by
the diluent temperature
Internal temperature of optical
15~40
system
9.2. Heating System
9.2.1 Diluent Heating System
Structure diagram
1. Temperature detection assembly
Symbol:
9-1
Heating System
Appearance:
Function:
a. Determine if the diluent temperature is within [10°C, 40°C]. If not, the analyzer
will stop working and beep; b. provides the diluent temperature to calculate the bath temperature.
2. Reagent preheating unit:
Symbol:
Appearance:
Function: perform and control heating of diluent, and detect the bath temperature after heating.
9-2
Heating System
9.2.2 Optical heating system
Optical System
Temperature Testing
Unit
J10
Analog Drive
Board
J17
J18
J19
Fan System
Function:
Power assembly: continuously heats the optical system;
Optical System: detects the temperature and determine whether to work;
Fan assembly: cools the optical system. Starts when the optical system temperature is
higher than 30℃, and stops when the optical system temperature is lower than 25℃.
9-3
10 Mechanical System
10.1. Mechanical System Overview
This section lists the locations of major analyzer components for the service personnel to
remove and replace the components. The diagrams in this manual is based on BC-5150.
BC-5000 and BC-5150 are basically the same.
Front view
Figure 10-1 Front view of the main unit
1 ---- Display
2 ---- Power/Status indicator
3 ---- Key supporting pad (wipe baffle)
4 ---- Sample probe
5 ---- [Aspirate] key
10-1
Mechanical System
Figure 10-2 Front view of the main unit (cover open)
1 --- Fluidic valve
2 --- Syringe
3 --- Metering pump
4 ---
5 --- Sampling assembly
6 --- Pressure & Temperature testing unit
Back view
10-2
[Aspirate] key
Mechanical System
Figure 10-3 Back view of the main unit
1 --- Diluent Inlet
2 --- Waste Outlet
3 --- Waste sensor
4 --- Power input socket
5 --- Power switch
6 --- Power backplane
7 --- Back panel
10-3
Mechanical System
Figure 10-4 Back view of the main unit (internal structure)
1 --- Analog drive board
2 --- Pinaster board
3 --- Fan assembly
Left view
Figure 10-5 Left view of the main unit
10-4
Mechanical System
1 --- Access door
2 --- Panel Module
3 --- Left door assembly
4 --- Network interface and USB port
Figure 10-6 Left of the analyzer (left door open)
1 --- Left door
2 --- DIFF reagent bottle
3 --- LH reagent bottle
4 --- Front Panel Module
5 --- Network port, USB port
10-5
Mechanical System
Left view
Figure 10-7 Right view of the main unit (right door open)
1 --- Fluid port
2 --- Waste water pump subassembly
3 --- Vacuum chamber assembly
4 --- valve
5 --- Temperature sensor
6 --- Sampling assembly
7 --- Panel Module
8 --- RBC unit
9 --- WBC unit
10.2. Components
10.2.1 Introduction
This section provides exploded view of the analyzer and material IDs for the service
personnel to understand the relationship between the components when removing and
replacing the components.
Note
All the material IDs listed in the “Material ID” column of all the parts lists are only used for the
service personnel to search the corresponding spare part ID. Please provide the spare part ID when
purchasing a spare part.
10-6
Mechanical System
10.2.2 Overall
Exploded view
Parts list
No.
Material ID
1
Material Description
Spare Part ID
Comments
Panel Module (BC-5150)
/
/
Top Cover (BC-5150)
/
/
Panel Module(BC-5000)
/
/
Top Cover (BC-5000)
/
/
The M3X8 Combination Bolt
/
/
Right Door
/
/
Assembly, Left door
/
/
Main Unit
/
/
115-014325-00
3
1
115-014323-00
3
4
/
2
5
115-015670-00
7
6
/
Screw, Flat Head Phillips M3X6
/
/
8
115-016034-00
DIFF Lyse Cap Assembly
115-016034-00
/
9
115-016035-00
LH Lyse Cap Assembly
115-016035-00
/
10
115-007635-00
801-3201-00053-00
/
M-68D CAP Component for
Diluent
11
115-013091-00
Waste Cap assm
115-013091-00
/
12
3102-20-69094
Diluent Assembly holder
801-3110-00167-00
/
10-7
Mechanical System
10.2.3 Main unit
Exploded view
10-8
Mechanical System
10-9
Mechanical System
Parts list
No.
Material ID
Material Description
Spare Part ID
Comments
1
/
Mainframe
/
/
2
/
syringe assembly
/
See Section
11.2.6
3
115-014601-00
1ml Pump(eject)
115-014601-00
/
4
115-015678-00
Switch assembly
115-015678-00
/
5
/
3-way Valve (Mindray)
801-3201-00003-00
/
6
0030-30-07587
2-way Valve (Mindray)
0030-30-07587
/
115-007667-00
/
mirco solenoid valve of
7
115-007667-00
two-way(higher operating
pressure)
8
/
exoteric sampling keystroke
801-3100-00194-00
/
8
043-002703-00
exoteric sampling keystroke
043-002703-00
/
9
/
key support plate
/
/
10
115-014598-00
200ul pump
115-014598-00
/
11
115-017730-00
liquid detect assembly
115-017730-00
/
12
115-015673-00
Vacuum/Pressure Chamber
115-015673-00
/
13
/
RBC isolated washer
/
/
14
/
/
/
/
RBC shielding bottom plate
welding piece
15
115-015672-00
Reagent preheating unit
115-015672-00
16
/
RBC count pool module
/
See Section
11.2.8
17
043-000711-00
filter
043-000711-00
/
18
/
Isolation chamber clamp
801-3003-00045-00
/
19
042-007065-00
RBC shielding box
042-007065-00
/
20
/
WBC heat preservation box
/
/
21
115-015676-00
Sample Probe Assembly
115-015676-00
/
22
/
right door
/
/
23
115-014726-00
TAKASAGO value assem
115-014726-00
/
24
/
GHB count pool module
/
LVMvalve assembly
115-015675-00
See Section
25
115-015675-00
26
/
27
115-015677-00
28
115-015680-00
29
/
waste water pump
subassembly
11.2.8
/
See Section
/
11.2.10
Temperature detection
assembly
115-015677-00
/
See Section
Power Assembly
15-015680-00
reagent bottle plate cosmetic
/
11.2.13
10-10
/
Mechanical System
No.
Material ID
Material Description
Spare Part ID
Comments
piece
30
115-016034-00
DIFF Lyse Cap Assembly
115-016034-00
/
31
/
Reagent bottle,500ml
/
/
32
/
Reagent bottle,220ml
/
/
33
115-015681-00
Assembly, Left door
/
/
34
115-016035-00
LH Lyse Cap Assembly
115-016035-00
/
35
/
Plate, fixing liquid detect board
/
/
36
051-001621-00
Liquid detect board PCBA
051-001621-00
/
37
043-000829-00
Reagent detection tube
043-000829-00
/
38
/
/
/
Male Lure Lock Ring,For MTLP
or LC23,Green
39
/
Plate,fixing liquid fetect board
/
/
40
051-001621-00
Liquid detect board PCBA
051-001621-00
/
41
043-000829-00
Reagent detection tube
043-000829-00
/
42
/
Optical System
115-018419-00
/
43
115-017923-00
assembly, fan
115-017923-00
/
44
/
Assembly, power back plate
/
/
back plate
/
/
/
USB grounging sheet
/
/
/
Conductive Fabric Gasket
/
/
051-001159-00
/
45
See Section
11.2.11
46
47
051-001159-00
Pinaster board(5
ALL_AM1808)
48
/
Analog Drive Board PCBA
115-018411-00
49
115-017477-00
3107 wire assembly
/
/
50
115-017475-00
tube package
/
/
51
023-000549-00
023-000549-00
/
801-3100-00226-00
/
Secure Digital Memory Card
8GB
52
/
Lithium battery 3V 35mAh
D12.5*2.0
10-11
Mechanical System
10.2.4 Panel Module (BC-5150)
Exploded view
Parts list
No.
Material ID
Material Description
Spare Part ID
Comments
1
043-002706-00
BC-5150 front cover
043-002706-00
/
2
045-000016-01
Touch Panel Defend cushion
/
/
3
/
Touch Panel
801-3110-00184-00
/
4
/
screen module(BC-5150)
/
5
/
The M3X8 combination Bolt
/
6
/
touch panel shield box
7
051-000881-00
6301 touch control board PCBA
11
/
115-018418-00
/
/
M4 Strap Natural Nylon Color
/
94ULV-2
8
051-000675-00
Indication board PCBA
051-000675-00
/
9
/
shield(5150)
/
/
10
/
/
/
Cross pinhead screw with washer
M3X10
10-12
Mechanical System
10.2.5 Panel Module (BC-5000)
Exploded view
Parts list
No.
Material ID
Material Description
Spare Part ID
Comments
1
043-002705-00
BC-5000 front cover
043-002705-00
/
2
045-000016-01
Touch Panel Defend cushion
/
/
3
021-000005-00
Touch Panel
801-3110-00184-00
/
4
/
screen module(BC-5000)
/
5
/
The M3X8 combination Bolt
/
6
/
Touch panel shield box
7
051-000881-00
6301 touch control board PCBA
11
/
115-018417-00
/
/
M4 Strap Natural Nylon Color
/
94ULV-2
8
051-001062-00
Indicator board PCBA
051-001062-00
/
9
/
Shield(5000)
/
/
10
/
/
/
Cross pinhead screw with washer
M3X10
10-13
Mechanical System
10.2.6 Syringe Assembly
Exploded view
Parts list
No.
Material ID
Material Description
Spare Part ID
Comments
1
024-000366-00
Stepping motorSST42D2120
024-000366-00
/
2
/
coupling
/
/
3
/
Rubber Ring + Positioning Sleeve
/
/
4
041-005167-00
special bolt 3
041-005167-00
/
5
115-011902-00
10ml syringe(3107)
115-011902-00
/
6
/
The M3X8 combination Bolt
/
/
7
/
the fixed plate of two injecter
/
/
8
115-012708-00
250ul syringe(with nozzle)
115-012708-00
/
9
/
801-3003-00015-00
/
/
/
/
/
Syringe Motor Position Sensor
Assembly
10
/
Screw, Pan Head W/Washer
Phillips M3X6
11
/
M3X12 Hexagon socket screws
10-14
Mechanical System
10.2.7 Sample Probe Assembly
Exploded view
Parts list
No.
Material ID
Material Description
Spare Part ID
Comments
1
/
Elevator Motor(2S42Q-05640A)
801-1805-00013-00
/
2
/
Upper Positioning Sleeve
/
/
3
/
M3X5 Hexagon Socket Set Screw
/
/
4
/
Transducer discreteness
801-3001-00055-00
/
5
/
SCREW M3X4
/
/
6
/
801-3003-00015-00
/
Syringe Motor Position Sensor
Assembly
7
/
Lower Positioning Sleeve
/
/
8
/
probe wipe
801-3102-00057-00
/
9
/
/
/
Cross pinhead screw with washer
M3X10
10
/
probe wipe clamp
801-3900-00096-00
/
11
/
Sample Probe
801-3101-00002-00
/
12
/
The M3X8 combination Bolt
/
/
13
/
probe press plate
/
/
10-15
Mechanical System
10.2.8 RBC count pool module
Exploded view
Parts list
No.
Material ID
Material Description
Spare Part ID
1
/
Add liquid connector
Comments
/
115-018414-00
2
/
3
/
pool cover
/
M3X6 Stainless Steel Cross Recess
/
/
Pinhead Screw
4
/
Count pool support plate
/
/
5
/
Count pool adjusting plate
/
/
6
/
RBC count pool
7
/
Front bath washer
9
/
RBC back pool
8
045-000809-00
Ruby Red Cell Counter(D50um)
/
115-018412-00
/
/
10-16
045-000809-00
/
Mechanical System
10.2.9 HGB count pool module
Exploded view
Parts list
No.
Material ID
Material Description
1
/
add liquid connector
Spare Part ID
Comments
/
115-018413-00
2
/
3
/
pool cover
/
M3X6 Stainless Steel Cross Recess
/
/
Pinheads Screw
4
/
Count pool support plate
/
/
5
/
Count pool adjusting plate
/
/
6
043-002923-00
HGB count pool
043-002923-00
/
7
/
HGB amp module
115-018415-00
/
10-17
Mechanical System
10.2.10 Pump Assembly
Exploded view
Parts list
No.
Material ID
Material Description
Spare Part ID
Comments
1
/
pump mounting plate
/
/
2
/
The M3X8 combination Bolt
/
/
3
/
Rubber Ring Positioning Sleeve
/
/
4
/
Rubber Ring
/
/
5
/
Rotation (Waste) pump
/
115-018416-00
6
/
cable of waste pump
/
10.2.11 Power Backplane Assembly
Exploded view
10-18
Mechanical System
Parts list
No.
Material ID
Material Description
Spare Part ID
Comments
1
/
power back plate
/
/
2
/
LockNut,1/4-28UNF,Red Nylon
/
/
3
/
/
/
/
/
/
/
Lock Nut, Panel
Mount,1/4-28UNF,White Nylon
4
/
5
/
Coded Lock Ring, Red
Coded Lock Ring, For FTLLB or
FTLB,White
6
/
Female Luer,1/4-28UNF,1/8ID
/
/
7
/
BNC socket
/
/
8
/
Screw, Flat Head Phillips M3X6
/
/
9
0030-10-13055
Filter power 115/250VAC6A panel
mount
10
M07-00131F---
FUSE Time-lag 250V 3.15AD5X20
10-19
Mechanical System
10.2.12 Optical System
Exploded view
Parts list
No.
Material ID
Material Description
Spare Part ID
Comments
1
051-001142-00
3107 Preamplifier
051-001142-00
/
2
/
Shield of Preamp PCBA
/
/
3
M07-00143S---
SWITCH
/
/
4
/
SUS316 Flat Washer - A Class 4
/
/
5
/
Laser control board
/
/
6
/
Coverplate of Optics Shield
/
/
10-20
Mechanical System
10.2.13 Power Assembly
Exploded view
Parts list
No.
Material ID
Material Description
Spare Part ID
Comments
1
/
power cover plate
/
/
2
051-001319-00
3107 Power Source PCBA
051-001319-00
/
3
/
Power bottom plate
/
/
4
/
The M3X8 combination Bolt
/
/
10.3. Removal and Installation
10.3.1 Tools
The following tools may be needed during removal and replacement of components:
Crosshead screwdriver (107)
Flathead screwdriver
Tweezers
Pliers
Cutting pliers
Hex wrench set
10.3.2 Preparation for Disassembly
Before disassembling the analyzer, please make the following preparations:
10-21
Mechanical System
Stop the blood tests. Adjust the sample probe to the horizontal sampling position. Shut
down the analyzer and disconnect all the connections with accessories and peripherals.
Disconnect the external power supply.
All the analyzer components and surfaces are potentially infectious. Take proper
protective measures for operation and maintenance.
The reagents are irritating to eyes, skin and diaphragm. Wear proper personal protective equipment (e.g.
gloves, lab coat, etc.) and follow safe laboratory procedures when handling them in the laboratory.
If the reagents accidentally spill on your skin, wash them off with plenty of water and if necessary, go see a
doctor; if the reagents accidentally spill into your eyes, wash them off with plenty of water and
immediately go see a doctor.
Please eliminate static electricity before disassembly. While removing the components with electrostatic
sensitive mark, please wear protective equipment such like an antistatic wrist strap or antistatic gloves
to avoid ESD damage to the components.
During reassembly, please connect the wires correctly and keep them in proper positions to avoid short
circuit caused by damaged wires.
Use screws of suitable models during reinstallation. Using wrong screws may result in equipment damage.
Furthermore, during usage after reinstallation, a wrong screw may become loose and fall off, resulting
in unexpected product damage or personal injury.
Please disassemble the equipment in the correct order. Failure to do so may result in irreversible damage
to the equipment.
Please make sure all connections have been disconnected before disassembling the components. Be careful
not to break the wires or the connectors during disassembly.
Please store the removed screws and other parts in separate places for reinstallation purpose. Be careful
not to drop, contaminate or lose these parts.
During disassembly, separate the materials by module to avoid misusing or missing materials during
reassembly.
During reassembly, please assemble first the components then the main unit. Be careful with the wire
connections. Place the wires in proper position.
10.4. Disassembling the Main Unit
During the disassembly, make sure the site is smooth without foreign materials to avoid screen
scratches.
10-22
Mechanical System
All operations must be done by professionals. Insulating gloves must be worn when servicing.
After assembly, check all the fluidic tubes. Folding is strictly prohibited.
10.4.1 Removing the Back plate
Place the analyzer flat on the table as shown below. Unscrew the 4 M3×8 combination
screws and remove the back plate.
10.4.2 Removing the Analog drive board PCBA
1. Remove the back plate in accordance with Section 10.4.1.
2. Remove the 3 M3×8 combination screws as shown below. Remove the top cover of the
shield box and disconnect the RBC_PLT signal wire connector.
3. Remove all the cables from the analog drive board as shown below. Unscrew the 8
M3×8 combination screws and remove the analog drive board.
10-23
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Check all the connections on the analog drive board and make sure there is
no mistakes.
2. Start the analyzer and power on the analog drive board. Check if the analog drive
board power and indicators are working properly.
3. Perform blank background count. If no alarm occurs, then the analog drive board is
successfully replaced. Otherwise troubleshooting is needed.
10.4.3 Removing the Pinaster board or the SD card
1. Remove the back plate in accordance with Section 10.4.1.
2. Remove all the cables from the Pinaster board as shown below. Unscrew the 6 M3×8
combination screws and remove the analog drive board.
3. As shown below, unscrew the 2 M3×8 combination screws and remove the USB ground
plate and conductive foam as a unit.
10-24
Mechanical System
4. Remove the SD card from the board.
Installation:
Reverse the removal procedure.
Verification: 1. Check all the connections on the Pinaster board and make sure there is no
mistakes.
2. Start the analyzer and power on the Pinaster board. Check if the Pinaster board
power and indicators are working properly.
3. Perform blank background count. If no alarm occurs, then the Pinaster board is
successfully replaced. Otherwise troubleshooting is needed.
10.4.4 Removing the power backplate Assembly
1. Remove the power backplate in accordance with Section 10.4.1.
2. Place the analyzer flat on the table as shown below. Unscrew the 4 M3x8
combination screws. Remove the diluent and waste tubes, and cables and ground wires
connected with the power board.
10-25
Mechanical System
During installation, verify that the ground wire is connected to the correct ground pin properly.
10.4.5 Removing the Fan Assembly
1. Remove the power backplate assembly in accordance with Section 10.4.4.
2. Remove all the cables from the fan as shown below. Unscrew the 2 M3×8
combination screws and remove the fan assembly.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the electrical connections are correct.
3. After startup, press the “
” button from the system menu, select
“Maintenance”→“Self-test”→“Fan Self-test”→click “Spin”. When the “Spin” column
displays “spin”, check if the fans on the both sides of the back plate power switch can spin
properly. If so, click the “Stop” button and check if the fan is stopped when the “Stop”
column displays “stop”.
10.4.6 Removing the Right Panel
Place the analyzer flat on the table as shown below. Unscrew the 2 M3x8 combination
screws and remove the back plate.
10-26
Mechanical System
10.4.7 Removing the RBC count pool module(FRU)
1. Follow step 1 and 2 in Section 10.4.2 to remove the top cover from the analog board
shield box and disconnect the RBC_PLT signal wire connector.
2. Remove the right door in accordance with Section 10.4.6.
3. As shown below, unscrew the 2 M3×8 combination screws and remove the RBC shield
box.
4. As shown below, rotate the RBC back pool 90° counterclockwise and remove the RBC
back pool. Use tweezers to remove the front bath washer and the Ruby Red Cell Counter(D50um).
10-27
Mechanical System
5. As shown below, rotate the secondary RBC bath 90° counterclockwise and remove the
secondary RBC bath. Use tweezers to remove the primary bath washer and the microsensor.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. Verify normal operations by starting the analyzer.
Before removing the reaction bath shield box, please adjust the sample probe position so that the
sample probe leaves the shield box. Otherwise the sample probe may bend or hurt the operator.
Install the Ruby Red Cell Counter(D50um) so that the concave of the sensor is facing the center of
the RBC count pool.
pool
10.4.8 Removing the Ruby Red Cell Counter(D50um)
1. Follow step 2 and 3 in Section 10.4.7 to remove the RBC shield box.
10-28
Mechanical System
2. As shown below, cut off the straps which fasten the tube between the reaction bath to the
isolation chamber filter. Rotate the secondary RBC bath 90° counterclockwise and remove the
secondary RBC bath.
3. As shown below, unscrew the 2 M3 screws which fasten the RBC count pool module.
Remove the RBC count pool module. Use tweezers to remove the ront bath washer and the Ruby
Red Cell Counter(D50um).
Note: Use angled tweezers to push the Ruby Red Cell Counter(D50um) out from the RBC
count pool center
Before removing the reaction bath shield box, please adjust the sample probe position so that the
sample probe leaves the shield box. Otherwise the sample probe may bend or hurt the operator.
Install the Ruby Red Cell Counter(D50um) so that the concave of the sensor is facing the center of
the RBC count pool.
pool
10.4.9 Removing the HGB amp module
1. Remove the right door in accordance with Section 10.4.6.
2. As shown below, unscrew the 2 M3×8 combination screws and remove the WBC heat
preservation box.
10-29
Mechanical System
3. Follow the steps in Section 10.4.1 to remove the back plate, and disconnect the HGB unit
connector.
4. As shown below, unscrew the 2 M3 screws which fasten the pool cover. Remove the lower
part of the HGB count pool module. Rotate the bath counterclockwise 90° and remove the HGB
amp module.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. Verify normal operations by starting the analyzer.
Before removing the WBC heat preservation box,, please adjust the sample probe position so that
the sample probe leaves the shield box. Otherwise the sample probe may bend or hurt the
operator.
10-30
Mechanical System
10.4.10 Removing the Liquid detect board PCBA
1. Place the analyzer flat on the table as shown below. Unlock and open the left access door.
Loosen the strap which fastens the reagent bottle tubes and move the tubes towards the top end of
the reagent bottle plate cosmetic piece. Remove the reagent bottle (with the tubes) and put it away.
2. Unscrew the M3×8 compound screw, remove the liquid sensor board installation kit and
PCBA assembly of the liquid sensor board.
3. Remove the pipe and wires of the liquid sensor board and cancel the liquid sensor board
PCBA.
Note: During the installation, connect the PCBA connector at the square hole to the DIFF
reagent pipe, connect the PCBA connector at the round aperture to the LH reagent pipe.
10.4.11 Removing the Reagent Bottle plate cosmetic piece
10-31
Mechanical System
1.Perform step1and step 2 of section 10.4.10, take off the liquid sensor board installation kit
and PCBA assembly of the liquid sensor board.
2.Unscrew the 3 M3×8 combination screws and remove the reagent bottle plate cosmetic
piece.
10.4.12 Removing the preheat Assembly
1. Follow step 1 and 2 in Section 10.4.9 to remove WBC heat preservation box.
2. As shown below, remove the tubes from the Reagent preheating unit . Unscrew the 2 M3x8
combination screws and remove the Reagent preheating unit . Pull the connector out from the
hole. Disconnect the cable connector of the Reagent preheating unit (leave the connector at the
left side for installation purpose).
10-32
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the electrical connections are correct;
3. After startup, press the “
” button from the system menu, select
“Maintenance”→“Temp.& Press. Calibration” and check if the “preheat bath temperature”
column is displayed in red.
Note: If the wire head retracts back from the hole, please follow the steps in Section 11.4.10
to remove the reagent bottle baffle.
Before removing the WBC shield box, please adjust the sample probe position so that the sample
probe leaves the shield box. Otherwise the sample probe may bend or hurt the operator.
10.4.13 Removing the Electromagnet Pinch Valve Assembly
1. Follow step 1 and 2 in Section 10.4.9 to remove the WBC shield box.
2. As shown below, remove the tubes from the pinch valve. Unscrew the 2 M3x12 screws and
remove the valve assembly. Pull the connector out from the hole. Disconnect the cable connector
of the Reagent preheating unit (leave the connector at the left side for installation purpose).
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the electrical connections are correct;
3. After startup, press the “
” button from the system menu, select
10-33
Mechanical System
“maintenance”→“Self-test”→“Valve Self-test” and click on valve 18 to see if it is working
correctly.
Note: If the wire head retracts back from the hole, please follow the steps in Section 11.4.10
to remove the reagent bottle plate cosmetic.
Before removing the WBC shield box, please adjust the sample probe position so that the sample
probe leaves the shield box. Otherwise the sample probe may bend or hurt the operator.
10.4.14 Removing the Right Valve Assembly
1. Remove the right door in accordance with Section 10.4.6.
2. As shown below, remove the tubes from the valve which needs service. Unscrew the 2
M3x12 screws and remove the valve assembly. Pull the connector out from the hole. Disconnect
the cable connector of the Reagent preheating unit (leave the connector at the left side for
installation purpose).
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. After startup, press the “
” button from the system menu, select
“maintenance”→“Self-test”→“Valve Self-test” and click on the number (which is printed on the
fluidics separator) to see if the corresponding valve is working correctly.
Note: If the wire head retracts back from the hole, please follow the steps in Section 11.4.10
10-34
Mechanical System
to remove the reagent bottle plate cosmetic.
10.4.15 Removing the Pump
1. Remove the right door in accordance with Section 10.4.6.
2. As shown below, remove the tubes from the pump. Unscrew the 4 M3x12 screws and
washers and remove the pump assembly. Pull the connector out from the hole. Disconnect the
cable connector of the Reagent preheating unit (leave the connector at the left side for installation
purpose).
4. As shown below, unscrew the 4 M3×8 combination screws and remove the pump.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
10-35
Mechanical System
3. Verify normal operations by starting the analyzer.
Note: If the wire head retracts back from the hole, please follow the steps in Section 11.4.10
to remove the reagent bottle baffle.
10.4.16 Removing the Vacuum Chamber Assembly
1. Remove the right door in accordance with Section 10.4.6.
2. Remove the tubes from the vacuum chamber. Unscrew the 2 M3×8 screws and remove the
vacuum chamber assembly.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. After startup, press the “
” button from the system menu, select
“Status”→“Temp.& Pressure” and check if the “Vacuum” column is displayed in red.
4. Verify normal operations by starting the analyzer.
10.4.17 Removing the Top Cover
As shown below, unscrew the 2 M3×8 screws. Lift and remove the top cover from the rear
edge.
10-36
Mechanical System
10.4.18Removing the Temperature detection assembly
1. Remove the top cover in accordance with Section 10.4. 17.
2. Remove the cable connectors from the temperature detection assembly.
Installation:
Reverse the removal procedure.
Verification: 1. Verify the tube connections and the electrical connections are correct.
2. After startup, press the “
” button from the system menu, select
“Status”→“Temp.& Pressure” and check if the “Diluent temperature” column is displayed
in red.
10.4.19 Removing the Sampling Probe Assembly
10-37
Mechanical System
1. Remove the right door in accordance with Section 10.4.6.
2. Follow the steps in Section 11.4.17 to remove the top cover, and disconnect the cable
connectors of the motor and the upper/lower sensors from the sample probe assembly.
3. As shown below, remove the tubes from the sample probe. Unscrew the 4 M3×12 screws
and washers, and remove the sample probe assembly.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. After startup, press the “
” button from the system menu, select
“Maintenance”→“Sample Probe Debug” to verify the three posiaons of the sample probe
(“Initial position”, “Up"," Middle position” and “Down position” of “RBC bath” and “WBC
bath”) and ensure the reliable operation of the sample probe.
4. Verify normal operations by starting the analyzer.
10.2.20 Replace the Horizontal Motor of the Sampling Probe
Assembly
1. Remove the sample probe assembly in accordance with Section 10.4.19.
2. As shown below, first remove 2 set screws, then remove the 4 M3×10 screws, the Lower
positioning sleeve and the motor.
10-38
Mechanical System
10.4.21 Replace the Vertical Motor of the Sampling Probe
Assembly
1. Remove the sample probe assembly in accordance with Section 10.4.18.
2. As shown below, unscrew the 1 M3×4 screw and remove the Transducer discreteness.
3. As shown below, first remove 2 set screws from the fixing hole, then remove the 4 M3×10
screws, the Upper positioning sleeve and the motor.
10.4.22 Removing the Probe wipe
1. After startup, press the “
” button from the system menu, select
“Maintenance”→“Sample Probe Debug”. Click on the “Initial position”, the “RBC bath”， then
click on the “Up” to adjust the sample probe to above the RBC bath.
10-39
Mechanical System
2. Follow step 1 and 2 in Section 10.4.7 to remove the right panel and the RBC shield box.
3. As shown below, remove the probe wipe clamp and the Probe wipe. Disconnect the tube from
the Probe wipe.
Installation:
Reverse the removal procedure.
Verification: 1. Verify the tube connection is correct.
2. After startup, press the “
” button from the system menu, select
“Maintenance”→“Sample Probe Debug” to verify the three posiaons of the sample probe
(“Initial position”, “Up", "Middle position” and “Down position” of “RBC bath” and “WBC
bath”) and ensure the reliable operation of the sample probe.
3. Start the analyzer and perform the sample probe cleaning sequence. Check if any
fluid flows out from the bottom of the wipe.
10.4.23 Replacing the Sample Probe
1. After startup, press the “
” button from the system menu, select
“Maintenance”→“Sample Probe Debug”. Click on the “Initial position”, the “RBC bath”， then
click on the “Up” to adjust the sample probe to above the RBC bath.
2. Remove the probe wipe in accordance with Section 10.4.22.
3. As shown below, remove the tubes from the sample probe. Remove the M3 screws
and the probe press plate, and remove the sample probe.
10-40
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Verify the tube connection is correct.
2. After startup, press the “
” button from the system menu, select
“Maintenance”→“Sample Probe Debug” to verify the three positions of the sample probe
(“Initial position”, “Up"," Middle position” and “Down position” of “RBC bath” and “WBC
bath”) and ensure the reliable operation of the sample probe.
10.4.24 Removing the Syringe Motor Position Sensor
1.
Place the analyzer flat on the table. Follow the steps in Section 10.4.10 to remove the
reagent bottle cosmetic piece, and disconnect the cable connectors of the photocoupler assembly
of the sampling assembly motor.
2. As shown below, unscrew the 4 M3×4 screws and remove the Syringe Motor Position
Sensor Assembly.
10-41
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the electrical connections are correct;
3. After startup, press the “
” button from the system menu, select
“Maintenance”→“Sample Probe Debug”→“Iniaal posiaon” so that the sample probe goes
back into the initial position. Then click on “RBC bath” and “WBC bath” respectively to
verify normal operations of the sample probe.
4. Verify normal operations by starting the analyzer.
10.4.25 Removing the transducer discreteness
1. Follow the steps in Section 10.4.17 to remove the top cover, and disconnect the cable
connectors from the photocoupler assembly of the sample probe assembly motor.
2. Remove the right door in accordance with Section 10.4.6.
3. Move the sample probe by hand to above the WBC bath. Cut the strap fastening the sensor
assembly with diagonal pliers and remove the cable connectors.
4. Move the sample probe by hand to the front side. As shown below, unscrew the M3×4
screws, and remove the sensor assembly from the front side.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the electrical connections are correct;
3. After startup, press the “
” button from the system menu, select
“Maintenance”→“Sample Probe Debug”→“Iniaal posiaon” so that the sample probe goes
back into the initial position. Then click on “Up”, “Middle position” and “Down position”
10-42
Mechanical System
respectively to verify normal operations of the sample probe.
4. Verify normal operations by starting the analyzer.
10.4.26 Removing the Panel Module
1. Place the analyzer flat on the table. Unlock and open the left access door.
2. Remove the right door in accordance with Section 10.4.6.
3. Remove the top cover in accordance with Section 10.4.17.
4. As shown below, unscrew the 6 M3×6 flathead screws. Remove the Panel Module and
place it flat on the table.
5. Follow the steps in Section 10.4.1 to remove the back plate, and disconnect the cable
connectors of the Panel Module from the Pinaster board.
6. Follow the steps in Section 10.4.10 to remove the reagent bottle baffle. Move the front
cover signal wire from the back to the front and remove it.
Note: During installation, the excess part of the signal wire shall be completely inserted into
the analyzer. Failure to do so will result in signal interference.
10.4.27 Removing the Indicator board PCBA
1. Follow step 1-4 in Section 10.4.26 to remove the top cover, and put it on the table. Remove
the cable connector from the indicator.
2. As shown below, unscrew the 2 M3×10 screws and remove the indicator board cover and
the indicator board PCBA.
10-43
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections are correct;
3. Start the analyzer and verify the indicators can be illuminated.
10.4.28 Removing the 6301 touch control board PCBA
1. Follow step 1-4 in Section 10.4.26 to remove the top cover, and put it on the table. Remove
the cable connector from the touchscreen drive board.
2. As shown below, unscrew the 2 M3×8 combination screws and remove the Touch panel
shield box.
3. As shown below, remove the cables from the touchscreen. Unscrew the 2 M3×8
combination screws and remove 6301 touch control board PCBA.
10-44
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections are correct;
3. Start the analyzer and verify normal operations of the touchscreen.
10.4.29 Removing the Touch Panel
1. Follow step 1 and 2 in Section 10.4.28 to remove the shield box and disconnect the cables.
2. As shown below, unscrew the 6 M3×8 combination screws which fasten the LCD Module,
and remove the Touch Panel (without disconnecting the cables).
3. Remove the touchscreen from the front cover.
Installation:
Reverse the removal procedure.
Verification: 1. Check if each connecting wire between the LCD Module and the Pinaster
board is locked.
2. Check if all the components are installed and fastened in position.
3. Calibrate the touchscreen.
4. Start the analyzer and verify normal operations of the touchscreen.
10-45
Mechanical System
10.4.30 Removing the LCD Module
1. Follow step 1-4 in Section 10.4.26 to remove the top cover, and put it on the table. Cut all
the straps fastening the screen assembly with diagonal pliers.
2. Follow step 1 and 2 in Section 10.4.28 to remove the Touch panel shield box and disconnect
the cables.
3. As shown below, unscrew the 6 M3×8 combination screws which fasten the LCD Module,
and the 2 screws which fasten the strap. Remove the LCD Module and disconnect all the cables
from the screen.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections are correct;
3. Start the analyzer and verify that the screen is working correctly.
10.4.31 Removing the Microswitch Assembly
1. Follow step 1-4 in Section 10.4.26 to remove the top cover, and put it on the table.
2. Remove the back plate in accordance with Section 10.4.1. Disconnect the connections
from the microswitch.
3. As shown below, unscrew the 2 M3×4 screws and remove the exoteric sampling keystroke
and the key support plate as a unit.
10-46
Mechanical System
4. As shown below, unscrew the 2 M3×8 combination screws and remove the microswitch
from the small hole in the front panel.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections are correct;
3. The Start key can be pressed down and released normally with an audible “click”.
4. Verify normal operations by starting the analyzer.
10.4.32 Removing the Syringe
1. Follow step 1-4 in Section 10.4.26 to remove the top cover, and put it on the table.
2. Remove the tubes from the syringe assembly.
10-47
Mechanical System
3. As shown below, unscrew the 4 M3×12 screws and flat washers. Remove the syringe
assembly and remove the cables from the syringe.
4. As shown below, unscrew the 3 M3×8 combination screws and special bolt 3, then remove
the syringe.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections and the tube connections are correct;
3. Verify normal operations by starting the analyzer.
10.4.33 Removing the Syringe Motor
1. Follow step 1-3 in Section 10.4.32 to remove the syringe assembly.
2. As shown below, first remove the 2 M3×12 hex screws which lock the coupler, then
remove the 4 M3×12 hex screws which fasten the motor. Finally, remove the motor.
10-48
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections and the tube connections are correct;
3. Verify normal operations by starting the analyzer.
10.3.34 Removing the Syringe Motor Position Sensor Assembly
1. Follow step 1-3 in Section 10.4.30 to remove the syringe assembly.
2. As shown below, unscrew the 2 M3×6 screws which lock the coupler, and remove the
motor position sensor assembly.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections and the tube connections are correct;
3. Verify normal operations by starting the analyzer.
10.4.35 Removing the liquid detect assembly
1. Follow step 1-4 in Section 10.4.26 to remove the top cover, and put it on the table.
10-49
Mechanical System
2. Remove the tubes from the hydraulic detection assembly, and disconnect the cable
connections from the top.
3. As shown below, unscrew the 2 M3×8 combination screws and remove the liquid detect
assembly.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. Verify normal operations by starting the analyzer.
10.4.36 Removing the Electromagnetic Pump/Valve from the
Front Panel
1. Follow step 1-4 in Section 10.4.26 to remove the top cover, and put it on the table.
2. Remove the tubes from the assembly.
3. As shown below, unscrew the 2 M3x8 combination screws and remove the electromagnetic
pump/valve. Pull the connector out from the hole. Disconnect the cable connectors (leave the
connectors at the left side for installation purpose).
10-50
Mechanical System
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections and the electrical connections are correct.
3. Verify normal operations by starting the analyzer.
10.4.37 Removing the Left Door Assembly
1. Place the analyzer flat on the table. Unlock and open the left access door.
2. As shown below, unscrew the 2 M3×8 combination screws and remove the left door
assembly.
Note: When installing the left door assembly, the hook at the bottom of the left door shall
engage the bottom plate so that the access door in the left door will not swing.
10-51
Mechanical System
10.4.38 Removing the Optical System
Please refer to Section 6.4.2 Replacement of the Optical System.
10.4.39 Removing the Optical System Cover
1. Remove the left door assembly in accordance with Section 10.4.37.
2. Remove the top cover in accordance with Section 10.4.17.
3. As shown below, unscrew the 4 M3×8 combination screws and remove the top cover of the
optical system shield.
4. Please refer to Section 6.4 Maintenance of the Optical System.
10.4.40 Replacing the RBC/WBC Isolation Chamber Filter
10-52
Mechanical System
1. Follow step 1 and 2 in Section 10.4.7 to remove the right panel and the RBC/WBC shield
box.
2. As shown below, unscrew the 1 M3×8 screw. Remove the clip on the chamber and
disconnect the tubes from the filter. Remove the isolation chamber filter.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the tube connections are correct;
3. Verify normal operations by starting the analyzer.
10.4.41 Replacing the Power Board PCBA
1. Remove the left door assembly in accordance with Section 10.4.37.
2. Remove the top cover in accordance with Section 10.4.17.
3. Remove all the cable connections from above the power box and place the DIFF reagent
bottle in a front position on the table.
4. As shown below, unscrew the 2 M3×8 combination screws Remove the power box
assembly and disconnect the connecting wires of the power socket from inside.
10-53
Mechanical System
5. As shown below, unscrew the 2 M3×8 combination screws on both sides and remove the
cover plate.
6. As shown below, unscrew the 4 M3×8 combination screws on both sides and remove the
power board PCBA.
Installation:
Reverse the removal procedure.
Verification: 1. Check if all the components are installed and fastened in position.
2. Verify the cable connections are correct;
10-54
Mechanical System
3. Verify normal operations by starting the analyzer.
10-55
11 Troubleshooting
Repair Guide
Error
Error
Trigger
Related
Potential
Code
Mechanism
Factors
Failure
Related
Screen
Comm 0x0100
unicatio
0001
n failure
1. Serial
communicati
Analog drive
Damaged
board
communicatio
on protocol
error.
/
2. No header,
between the digital board J81
and the analog drive board J31.
Damaged
For regular problems:
communicatio
jumbo
n module
1. Check the connection
frames,
insufficient
Connection
frame length,
between the
checksum
control board
error,
For occasional problems:
1. Check the connection
n module
Control board
Troubleshooting Procedure
between the control board J81
Loose wire
and the analog drive board J31.
2. Replace the related boards.
J81 and the
unrecognizabl analog board
J31
e command
Control 0x0100
board
failure
0002
AD is not
Control board
within
Analog drive
&
board
Current
[2.44-2.55]
/
Power board
and connecting
wires
Voltage
1. Check if the parameters are
within normal ranges in the
Voltage & Current Status screen
Status
2. If so, replace the control
screen
board
3. Otherwise, check if the power
board and connecting wires and
the connection between the
analog drive board J2 and
control board J86 are normal
4. If so, replace the analog drive
board
11-1
Troubleshooting
System
clock
0x0100
0004
failure
Check the
Button battery
/
/
1. Check if the button battery is
system clock
installed on the digital board
is earlier than
2. If the battery is installed,
January 1,
2000
Control board
replace the battery and reset date
/
and time in the Setup screen.
Save and exit and reset the
analyzer
3. If the problem persists,
replace the control board
/
Diluent 0x0100
The diluent
Reagent
is used
level is lower
information
Setup
Reagent Setup screen
setup
screen
2. Verify there is sufficient
0104
up
than 4%
Reagent
1. Verify there is no alarm in the
reagent in the diluent tank
LH lyse 0x0100
is used
0105
up
The LH lyse
level is lower
than 10%
Reagent
1. Metering
Reagent
information
pump failure
Setup
Reagent Setup screen
setup
2. Leaking or
screen
2. Verify there is enough reagent
folding lyse
DIFF
0x0100
The DIFF
lyse is
0106
lyse level is
used up
1. Verify there is no alarm in the
in the reagent tank
related tubes
and valves
lower than
3%
Waste
0x0100
level is
0110
Float status
Waste sensor
Unable to
Sensor
1. Check if the indicated
assembly
change the
Status
information is as expected from
status
11-2
Troubleshooting
full
correctly due
screen
to foreign
the Sensor Status screen
2. Check if the connecting wire
matter on the
of the waste sensor is correct
float
3. Check if the float is able to
change the status correctly
BNC
connector of
the waste
sensor is not
connected
Power
0x0100
failure
0201
56V power is
Power board
Damaged
power board & Current
not within
[47, 60]
0x0100
12V power is
0202
not within
Voltage
Power cable
Unreliable
1. Check if the Voltage &
Current Status screen shows
Status
expected information
screen
2. Check if the voltage at the
test port of the related power
power cable
board is as expected
[11.5, 12.5]
0x0100
-12V power
Analog drive
Damaged
0203
is not within
board
board
3. Check if the connection
between the power board and
the analog drive board is normal
[-12.5, -11.5]
4. Check if there is no short
0x0100
0204
P12V power Peripherals and Short circuit
circuit in analog peripherals,
is not within
connection power drive
connections
[11.5, 12.5]
tempera
/
connections
/
0x0100
P24V power
0205
is not within
5. Replace the analog drive
[22.0, 29.0]
board
preheat 0x0100
bath
peripherals and their respective
0401
The preheat
preheat bath
bath
temperature
ture
reading at the
sensor
analog drive
failure
board is 0 or
70
Temperature
Damaged
Temp.&
1. Verify the corresponding
temperature
Pressure
temperature value from the
sensor
Status
Status screen
screen
2. First, check if the ambient
Loose wire
temperature is overheating
sensor
3. If the temperature reads “0”,
connection
11-3
Troubleshooting
Optical 0x0100
The optical
assembl
assembly
0402
y
Optical system
temperature
tempera
reading at the
ture
analog drive
sensor
board is 0 or
failure
70
Diluent 0x0100
The optical
tempera
assembly
0403
ture
temperature
sensor
reading at the
failure
analog drive
Damaged
the temperature sensor is
temperature
shorted. Replace related
components
sensor
Temperature
4. If the temperature reads “70”,
Loose wire
the temperature sensor is open.
sensor
First check for an unreliable
connection
connection, then replace related
Temperature
Loose wire or
sensor
damaged
connection
temperature
components
sensor
board is 0 or
70
preheat 0x0100
After startup,
assembl
the preheat
0404
y failure
preheat
Damaged
assembly
heater
bath
Status
screen
then there is an assembly failure
temperature
rises less than
2. If the problem persists after
sensor
1 degree per
preheat bath temperature is less
than 35℃ or greater than 55℃,
Damaged
temperature
1. Enter the Status screen. If the
reconnecting the wires, replace
minute for
Damaged
consecutive 3
the preheat assembly
temperature
minutes
switch
Assembly
Loose wire
related
connections
Laser
0x0100
failure
0809
Laser current Optical system
is not within
[20, 70]
Damaged
Voltage
laser
& Current
Laser drive
Damaged
board
board
1. First, check for an abnormal
laser current from the Status
Status
screen
screen
2. Open the optical system
shield box and turn the box
Optical system
Damaged
shield box
11-4
switch on and off manually. If
Troubleshooting
switch
the laser is not illuminated,
check in turn if the connections
Optical system
Loose wire
of optical system, box switch
related
and laser are reliable. If there is
connections
no connection problem, check if
the laser drive board and analog
drive board are working
properly according to the
hardware troubleshooting
procedures.
3. If the laser is illuminated
properly, then check if the laser
drive board is working properly
according to the hardware
troubleshooting procedures.
Otherwise, replace the optical
system.
4. If the laser is damaged,
replace the optical system
Syringe
/
Photocoupler
Damaged
System
1. Confirm the problem from
assembl
photocoupler
Self-test
the System Self-test screen
y failure
or dirty
2. Check the wires, the
surface
photocoupler, the motor and
Syringe motor
Damaged
motor
Related
Loose wire
connections
Drive
Movement
mechanism
limited by
mechanical
interference or
other causes
11-5
motion interference
Troubleshooting
/
Sampli
ng
/
Vertical
photocoupler
assembl
Lifting motor
y lifting
System
1. Confirm the problem from
Self-test
the System Self-test screen
2. Check the wires, the
/
photocoupler, the motor and
mechan
Mechanical
Motion
ism
stop
interference
Related
Loose wire
failure
motion interference
connections
/
Sampli
ng
/
Horizontal
photocoupler
assembl
Self-test
the System Self-test screen
2. Check the wires, the
photocoupler, the motor and
motor
swingin
motion interference
g
Mechanical
Motion
mechan
stop
interference
Related
Loose wire
ism
failure
connections
Backgr 0x0100
ound
1. Confirm the problem from
/
Deflecting
y
System
0702
abnorm
Background
Reagent
unqualified at
contaminated
startup
ality
1. Verify the reagent quality
Reagent
2. If the background PLT is too
or expired
Analyzer tubes
high, verify the impedance
channel shield (please refer to
Abnormal
the treatment of impedance
shutdown
channel signal interference
Bad
alarm)
maintenance
3. Verify the cleanness of the
practice
reaction bath and correct
maintenance practice
4. Check there is no leaks in
tube connections and valves
HGB
blank
0x0100
HGB voltage WBC reaction
in not within
There is
Voltage
foreign matter & Current
11-6
1. Check there is no foreign
Troubleshooting
voltage
0801
[3.2, 4.9]
bath
abnorm
in the bath
The bath is
al
Status
matter in the WBC bath
screen
2. Check the bath is correctly
filled with reagent
not filled with
3. Verify the HGB blank voltage
diluent while
from the Voltage & Current
measuring
Analyzer setup
Status screen
HGB gain
4. Verify the HGB gain settings
setup incorrect
HGB assembly
5. Check the HGB assembly
Damaged
HGB
assembly
Fluid
0x0100
pressure
0803
overloa
d
MCU reports
Hydraulic
Related tubes
absolute
sensor related
folded
pressure
tubes
Valve
above 320
Status
screen
Status screen
failure
Optical system
close to current local
atmospheric pressure from the
blockage or
kPa
1. Check if the fluid pressure is
2. Check there is no folding
tube, blocked or broken valve in
Severely
analyzer tubes
blocked
3. Check if there is obstruction
Hydraulic
Loose
sensor and
connection or
connection
damaged
in the sample probe or optical
system
4. Check if there is loose
sensor
connection in hydraulic sensor
and its connecting wire
Abnor
0x0100
mal
0804
vacuum
Vacuum is
Waste pump
not within the
Dirt blockage
or failure
range
Status
screen
1. Enter the Status screen.
Check if the pressure building
process is normal (e.g. if the
specified by
Related
Dirt blockage
the sequence
solenoids
or failure
Related tubes
Leakage
waste pump is working
properly)
2. Check if the pressure can be
maintained from the Status
screen. If not, check if there is
Analog drive
Damaged
11-7
gas leaking in related tubes or
Troubleshooting
board
board
valves;
3. If pressure building fails,
check if the waste pump fails or
if the tubes are leaking
4. If the waste pump is not
working properly, check if the
related connection and the
analog drive board is working
correctly
Abnor
0x0100
preheat bath
Temperature
Loose wire or
mal
0805
temperature
sensor
damaged
tempera
out of range
connection
temperature
temperature is as expected from
ture of
[expected
sensor
the Status screen. Determine if
preheat
temperature
the abnormal status is caused by
-1.5, expected
too great temperature difference
temperature
during diluent replacement
+3]
(recoverable, not a problem)
bath
Status
1. Check if the preheat bath
screen
temperature and the diluent
2. Solve the problem of the
sensor according to Temperature
Sensor Failure
Refer to preheat Assembly Failure
preheat
assembly
High
0x0100
analyze
0806
r
tempera
ture
Optical
Fan assembly Damaged fan
Status
or loose wire
screen
temperature is
above 40℃
Analyzer air
Air
inlet/outlet
inlet/outlet
blockage
1. Check if the optical system
temperature is as expected from
the Status screen
2. Check if the fan assembly is
working correctly (check for fan
assembly damage or analog
Optical system Loose wire or
damaged
temperature
sensor
11-8
drive board damage), and if the
air inlet/outlet is blocked
3. Solve the problem of the
Troubleshooting
log drive board
sensor according to Temperature
Damaged
Sensor Failure
board
Diluent 0x0100
Diluent
tempera
temperature
080C
ture
above 40℃
above
upper
Fan assembly Damaged fan
Status
or loose wire
screen
Ambient
Room
Temperature
temperature
temperature is as expected from
the Status screen
2. Check if the fan assembly is
working correctly, and if the air
too high
limit
1. Check if the diluent
inlet/outlet is blocked
Temperature
Loose wire or
sensor
damaged
connection
temperature
3. Solve the problem of the
sensor according to Temperature
Sensor Failure
sensor
4. Check if the ambient
temperature is within the
specified operation temperature
range of the product
Diluent 0x0100
Diluent
tempera
temperature
080D
ture
below 10℃
below
lower
Ambient
Temperature
Temperature
too low
Diluent
Temperature
temperature
too low
Status
screen
1. Check if the diluent
temperature is as expected from
the Status screen
2. Solve the problem of the
sensor according to Temperature
limit
Sensor Failure
3. Check if the ambient
temperature is within the
specified operation temperature
range of the product
Blocka 0x0100
ge
0901
Aperture
RBC bath
Aperture
System
voltage is too
assembly
blockage or
Self-test
high (24V) or
changes
dramatically
bad
connection
between
primary bath
and secondary
11-9
/
Troubleshooting
bath
Apertur 0x0100
e
0902
voltage
Aperture
Aperture
voltage below
17V
is too
(temperature
low
corrected)
Wrong
System
1. Run aperture voltage test
aperture
Self-test
from the System Self-test screen
specification
Diluent
Loose wire or
temperature
damaged
sensor
temperature
assembly
sensor
to verify low aperture voltage
2. If the aperture has been
replaced, check if there is wrong
aperture specification,
installation problem or cracked
aperture
3. Troubleshoot the temperature
sensor according to Diluent
Temperature Sensor Failure
Impeda 0x0100
nce
0903
channel
PLT noise
RBC bath
Loose shield
proportion
assembly
wire
RBC bath
Loose shield
shield box
box
Lab
Unreliable
electromagneti
ground
c environment
connection
Analog drive
Loose
board
impedance
over 10%
signal
interfer
ence
/
/
/
1. Problem 1: Clean the flow
channel shield
box
Abnor
0x0100
mal
0904
optical
Abnormal
Flow cell
Dirt blockage
sample
cell
stream
2. Problem 2: Replace the
system
optical system
signal
11-10
12 Debug
12.1 Mechanical Position Adjustment
Figure 1.1
Tooling
Fixture
Materials
Consumables
Operation
Procedure
1.1
1.2
Figure 1.2
Figure 1.3
1.3
Operation Content
Click on “Service → Sample Probe Debug”. Enter the
Debug screen as shown in Figure 1.1.
First, click on the “Initial position” so that the sample
probe is above the RBC bath, as shown in Figure 1.1.
Click on the “WBC bath”(or “RBC bath”) button from
the screen shown in Figure 1.1. Click the “Middle
position” button so that the probe wipe is shown.
Check if the sample probe is basically in the center of
the bath. If there is any deviation, loosen the four
screws shown in Figure 1.3 and slightly adjust the
bath in left/right and front/back directions so that the
sample probe is in the center of the bath. Then
tighten one screw in each direction.
Quality Control
QC Point
Adjust the
sample probe
position
Fine tuning of
the relative
position
between the
sample probe
and the reaction
bath
QC Standard
The sample
probe is
basically in
the center of
the bath
Adjustment screw for front and
back position
Adjust
ment
screw
for left
and
right
positio
n
Note:
1. In Figure 1.1, before clicking on Initialization, all the buttons except the “Initialization” button will be
grey. Only after clicking on Initialization, the three buttons in the “Sample probe horizontal position”
column will be illuminated and available for use. The “Middle position” and “Down position” buttons will
not be available until clicking on the “WBC bath” or “RBC bath” button.
12-1
Debug
12.2 Commissioning of detection elements
12.2.1 Calibrate and verify the preheat temperature
Process
Temperature Calibration
Figure 2.1
Materials
Tooling Fixture
Operation
Procedure
2.1
Machine
measurement value
Fill in the sum of the
machine measurement
value and the FRU value
2.2
12-2
Operation Content
Click the Start Menu bar, then
click “Service→
Temp.&
Press. Calibration” to enter the
“Temperature calibration”
screen. Click on the “Preheat
bath temperature calibration”
button to enter the screen
shown in Figure 2.1
Record the machine
measurement value, and enter
the sum of the machine
measurement value and the
value on the service FRU as the
meter measurement value
Quality Control
QC Point
Measure the
preheat bath
temperature
calibration
factor
QC Standard
Debug
12.2.2
Counting channel measurement
Figure 3.1
Tooling
Fixture
Crosshead
screwdriver
Operation
Procedure
3.1
3.2
3.3
Operation Content
Open the reaction bath shield box, run
several blank counts in the
“WB-CBC+DIFF” mode.
Ensure that there is no gas bubble in the
sample tube and no fluid residue in the
sample probe during a complete count.
Otherwise, replace the sample tube.
Check there is no gas in the sample supply
tube and no bubble in the WBC/RBC bath
related tubes.
Check if the fluid level of the WBC and
RBC reaction bath is normal during the
whole count process; if the probe tip is
below the fluid level every time the probe
enters the bath; if there is any splash or
bubble when adding fluids; if the bubbles
contact with the filling tube; if the WBC
and RBC bath can be drained correctly.
12-3
Materials
Quality Control
QC Point
QC Standard
Sample
tube,
sample
probe, DIFF
bath
No gas bubble in the sample
tube, no fluid residue in the
sample probe, no gas in the
sample supply tube and no
bubble in the related tubes.
WBC and
RBC
reaction
bath
There is no splash when WBC
or RBC bath is filled; bubbles
can be correctly generated;
bubbles do not contact with the
filling tube; the probe tip is
below the fluid level every time
the probe enters the bath; the
WBC and RBC bath can be
correctly drained; no fluid
residue is on the bath wall.
Debug
3.4
3.5
3.6
Click “Service→Self-test” from the main
menu bar to enter the “Self-test” screen,
as shown in Figure 3.1. Click on the
“Aperture voltage” button and record the
date generated in the Status Screen in the
record sheet
Installed the shield cover with M3×8
stainless steel panhead combination
screws and tighten the screws with a
crosshead screwdriver.
Run a count again and observe the sample
probe assembly during the whole process.
The sample probe shall not interfere with
the shield box while the reaction bath is
mixing.
Aperture
voltage
Aperture voltage is normal and
no alarm is triggered.
Tighten the
screws
The relative
position
between
the sample
probe and
the reaction
bath shield
box
The relative position between
the sample probe and the
reaction bath shield box
Note: The above-mentioned “gas bubble” refers to big bubble which separates the fluid in the tube.
12-4
13 Commissioning and Verification
After Servicing
Component Name
Material ID
Commissioning
Items
Requirements After
Replacement
Pinaster board(5
051-001159-00
ALL_AM1808)
1. Perform data
Version
backup according to
information is
the prompts
correct
2. Check the
corresponding version
information from the
“Version Info.” screen
Analog Drive Board
115-018411-00
1. Perform HGB gain
calibration
HGB blank
voltage is 4.5V
2. Recalibrate the
MCV gain
Syringe transfer assembly
115-020624-00
10ml syringe
115-011902-00
Optical Sensor block of
3101-20-68304
Recalibration
Verify the
reproducibility
injector
special bolt 3
041-005167-00
10ml syringe FRU
115-015338-00
250ul syringe(with
115-012708-00
nozzle)
Stepping motor
024-000366-00
Sampling Assembly
115-015676-00
Elevator
801-1805-00013-
Motor(2S42Q-05640A)
Rotation Motor Position
Sensor Assembly
sensor package
sampling assembly
horizontally and
00
801-3003-00015-
vertically to ensure
that the sample line is
00
801-3001-00055-
unobstructed with no
folding and
00
Ф1.5 mop FRU
1. Move the
801-3102-00057-
13-1
interference with the
Verify the
reproducibility
Commissioning and Verification After Servicing
00
Sample Probe(for
BC-5300 only)
pre-amplifier, fluidics
801-3101-00002-
separator, right door,
motor, valves and line
00
straps
2. Ensure the sample
line is not squeezed or
deformed at the line
straps
3. Ensure there is no
folding or interference
when the wipe fluid
line is moving
horizontally or
vertically in the
sample assembly
4. Mechanical
commissioning of the
relative position
between the sample
probe and the
WBC/RBC bath
5. Recalibration
RBC cound pool
115-018412-00
module(FRU)
1. Mechanical
commissioning of the
relative position
between the sample
probe and the bath
2. The waste line for
the reaction bath needs
to be wrapped in the
vertical direction to a
height above the liquid
level with 3mL of
liquid in the bath
3. Perform HGB gain
13-2
Verify the
reproducibility
Commissioning and Verification After Servicing
calibration
4. Recalibration
HGB cound pool module
115-015987-00
1. Mechanical
commissioning of the
relative position
between the sample
1. Verify the
reproducibility
2. HGB blank
voltage is 4.5V
probe and the bath
2. The waste line for
the reaction bath needs
to be wrapped in the
vertical direction to a
height above the liquid
level with 3mL of
liquid in the bath
3. Perform HGB gain
calibration
Front bath washer
(molding WH02-205)
Microsensor (D50µm)
801-3005-0005700
045-000809-00
1. Install the aperture
1. Verify the
so that the surface with
reproducibility
the tapered bore faces
2. Verify the
the primary bath
aperture voltage
2. Recalibration
RBC shielding bottom
042-007064-00
plate welding piece
Mechanical position
Verify that the
adjustment of RBC
sampling assembly
bath
will not contact
with the RBC bath
when moving
vertically in the
RBC bath position
HGB FRU
115-018415-00
Perform HGB gain
calibration
Vacuum unit
115-015673-00
Waste pump assembly
115-018416-00
/
HGB blank
voltage is 4.5V
Verify that the
reaction baths can
be correctly
(FRU)
drained and
vacuum can be
13-3
Commissioning and Verification After Servicing
correctly built
2-way Valve (Mindray)
801-3201-00002-
/
00
2-way Valve (Mindray)
1. Verify the
orientation of the
inlet/outlet is
801-3201-00004-
correct;
00
2. The hose shall
be fully inserted;
3. Thick 50 tubes
can not be used
again after
disconnected from
valve ports or
connectors
3-way Valve (Mindray)
801-3201-00003-
/
00
1. Verify the NO,
NC and Common
terminals are
connected
correctly;
2. The hose shall
be fully inserted;
3. Thick 50 tubes
can not be used
again after
disconnected from
valve ports or
connectors
LVM valve assembly
115-015675-00
Recalibration
1. Verify the NO,
NC and Common
terminals are
connected
correctly;
2. The hose shall
be fully inserted;
3. Verify the
reproducibility
13-4
Commissioning and Verification After Servicing
1ml Pump(eject)
115-014601-00
Recalibration
/
200ul pump
115-014598-00
Recalibration
/
TAKASAGO value assem
115-014726-00
/
1. Verify the
pinch valve
assembly (T34) is
properly positioned
in the valve;
2. Cut T75 and
case it between
T34 and C11
BC-5000 front cover
043-002705-00
BC-5150 front cover
043-002706-00
Touch Panel
801-3110-00184-
Perform touchscreen
calibration
Verify normal
operations of the
touchscreen
00
LCD Module(5000)
115-018417-00
LCD Module(5150)
115-018418-00
Reagent preheating unit
115-015672-00
1. J9 and C10 must
be full inserted
2. FRU record
Verify the
temperatures are
within the ranges
parameters enters new
from the Status
temperature value
screen
from the Temperature
Calibration screen
Battery Lithium 3V
35mAh D12.5*2.0
801-3100-0022600
Reset date and time
After startup, the
in the Setup screen.
date remains the
Save and reset the
same
analyzer
Fan assembly
115-017923-00
/
Verify if the fan
rotates
Secure Digital Memory
023-000549-00
Card 8GB
1. Backup the data
/
before replacing the
SD card (if possible);
2. Restore the
configuration data;
exoteric sampling
043-002703-00
13-5
Adjust the 2 M3 hex
1. Verify there is
Commissioning and Verification After Servicing
keystoke
Start key for open-vial
mode
801-3100-0019400
screws which fasten
a uniform gap
the aspirate key, in
between the key
order to achieve a
and the front cover
uniform gap between
the key and the front
2. Key operation
is normal
cover
tube package
115-017475-00
/
See the Fluidic
System Precautions
for Assembly and
Service section in
the Service Manual
connector package
115-017476-00
/
See the Fluidic
System Precautions
for Assembly and
Service section in
the Service Manual
13-6
14 Service BOM
Part N.O.
Part Name
051-001159-00
Pinaster board (5 ALL_AM1808)
115-018411-00
Analog Drive Board
051-001319-00
3107 Power board PCBA
051-001062-00
Indicator board PCBA
051-000881-00
6301 touch control board PCBA
115-020624-00
Syringe transfer assembly
115-011902-00
10ml syringe
3101-20-68304
Optical Sensor block of injector
041-005167-00
special bolt 3
115-015338-00
10ml syringe FRU
115-012708-00
250ul syringe(with nozzle)
024-000366-00
Stepping motor
115-015676-00
Sampling Assembly
801-1805-00013-00
Elevator Motor(2S42Q-05640A)
801-3003-00015-00
Rotation Motor Position Sensor Assembly
042-007187-00
probe press plate
801-3001-00055-00
sensor package
801-3102-00057-00
Ф1.5 mop FRU
801-3101-00002-00
Sample Probe(for BC-5300 only)
3005-20-44746
probe wipe clamp
115-018412-00
RBC count pool module(FRU)
042-007065-00
RBC shielding box
115-015987-00
HGB count pool module
Service BOM
043-000711-00
filter
801-3003-00045-00
Isolation chamber clamp
801-3005-00057-00
Preventive Kit
045-000809-00
Ruby Red Cell Counter(D50um)
M6M-010063---
Seal for pressure sampling connector
042-007064-00
RBC shielding bottom plate welding piece
115-018414-00
RBC pool cover
115-018415-00
HGB FRU
115-015673-00
Vacuum unit
115-018416-00
waste water pump assembly
801-1805-00006-00
Vacuum chamber assembly
801-3201-00002-00
2-way Valve (Mindray)
801-3201-00003-00
3-way Valve (Mindray)
801-3201-00004-00
2-way Valve (Mindray)
115-015675-00
LVM valve assembly
115-014601-00
1ml Pump(eject)
115-014598-00
200ul pump
115-014726-00
TAKASAGO value assem
043-002705-00
BC-5000 front cover
043-002706-00
BC-5150 front cover
801-3110-00184-00
Touch Panel, resistive, 10.4″, four wire
115-018417-00
LCD Module(5000)
115-018418-00
LCD Module (5150)
115-015672-00
Reagent preheating unit
115-015678-00
Micro-switch assembly
M07-00131F---
FUSE Time-lag 250V 3.15AD5X20
0030-10-13055
Power filter
14-2
Service BOM
115-015677-00
Diluent temperature testing unit
115-017730-00
liquid detect assembly
801-3100-00226-00
Battery Lithium 3V 35mAh D12.5*2.0
115-017923-00
Fan assembly
509B-10-05996
Chinese power cord
801-3201-00053-00
M-68D CAP Assembly
115-016034-00
DIFF Lyse Cap Assembly
115-013091-00
Waste bottle cap assembly
801-3110-00167-00
Diluent Assembly holder
023-000549-00
Secure Digital Memory Card 8GB
110-002557-00
Installer CD
043-002703-00
exoteric sampling keystoke
801-3100-00194-00
Start key for open-vial mode
115-017475-00
tube package
115-017476-00
connector package
051-001621-00
Liquid detect board PCBA
043-000829-00
Reagent detection tube
14-3
15 Appendices
A.
Fluidic diagram
A-1
B.
Connection and Tube
ID
Material
type
1
2
3
4
5
6
7
syringe
WBC
RBC
Vacuum
Pump
probe
probe wipe
chamber
8
9 Valve
10 Valve
Valve
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
Valve
Valve
Valve
Valve
Valve
Valve
Valve
Valve
Valve
Valve
Valve
Valve
Valve
valVe
Name in
the
diagram
Part NO.
Name
SR
WBC
RBC
VC
LP
SPB
SPB
Isolation
chamber1,I
solation
chamber2
SV1
SV2
115-015652-00
115-015987-00
115-015986-00
115-015673-00
115-015674-00
3101-20-68488
3102-20-69178
syringe assembly
GHB cound pool module
RBC cound pool module
Vacuum/Pressure Chamber
waste water pump subassembly
sampling probe
probe wipe
115-002439-00
Isolation chamber filter
115-010088-00
115-010088-00
SV3
115-007667-00
115-010089-00
115-010089-00
115-010089-00
115-010089-00
115-010088-00
115-010089-00
115-010089-00
082-000446-00
115-010089-00
115-010089-00
115-010089-00
115-010089-00
115-010089-00
115-014726-00
3-way Valve (Mindray)
3-way Valve (Mindray)
mirco solenoid valve of two-way(higher
operating pressure)
2-way Valve (Mindray)
2-way Valve (Mindray)
2-way Valve (Mindray)
2-way Valve (Mindray)
3-way Valve (Mindray)
2-way Valve (Mindray)
2-way Valve (Mindray)
Valve.3-way -75~250kPa DC12V
2-way Valve (Mindray)
2-way Valve (Mindray)
2-way Valve (Mindray)
2-way Valve (Mindray)
2-way Valve (Mindray)
TAKASAGO value assem
115-017730-00
liquid detect assembly
SV4
SV5
SV6
SV7
SV8
SV9
SV10
SV11
SV12
SV13
SV14
SV15
SV16
PV18
Hydraulic
Sensor
26
Sensor
Preheat
Preheat Bath
27
Bath
28 Pump
DP1
29 Pump
DP2
30 Valve
CV1
Temperatur
31 Sensor
e Sensor
32 Filter
LF
33 /
/
34 /
/
35 Tube
T1
36 Tube
T2
37 Tube
T3
38 Tube
T4
39 Tube
T5
40 Tube
T6
41 Tube
T7
42 Tube
T8
43 Tube
T9
44 Tube
T10
45 Tube
T11
46 Tube
T12
47 Tube
T13
Position
in the
Fluidic
diagram
D2
B3
B4
D5
D4
D2
D2
C3,C4
A4
A4
C2
B2
C2
A2
B5
A4
D3
C3
C2
B5
C5
C3
C3
C2
B3
C2
115-015672-00
Preheat Bath
A3
115-014601-00
115-014598-00
BA40-10-62038
1ml Pump(eject)
200ul pump
Check Valve A of WU
A4
B4
A3
115-015677-00
0010-10-12408
/
/
082-000108-00
082-000108-00
082-000108-00
0040-10-32301
M90-100031--M90-100031--M90-100031--M6G-020007--082-000108-00
082-000108-00
082-000108-00
082-000108-00
M90-100071---
Diluent temperature testing unit
B1
Inline Filter 43um 1/8” I.D. Tubing
A5
/
/
/
/
Tube.M-87-D3,2mmX3.5mm,AV31X2103
C1
Tube.M-87-D3,2mmX3.5mm,AV31X2103
C1
Tube.M-87-D3,2mmX3.5mm,AV31X2103
B1
Tubing. FEP,0.040"X0.066"（1feet）
D2
tube.PTFE,1.7mmIDX2.55mmOD
C2
tube.PTFE,1.7mmIDX2.55mmOD
C2
tube.PTFE,1.7mmIDX2.55mmOD
C2
Tube OD3mm ID1mm EVA
C2
Tube.M-87-D3,2mmX3.5mm,AV31X2103
C1
Tube.M-87-D3,2mmX3.5mm,AV31X2103
C1
Tube.M-87-D3,2mmX3.5mm,AV31X2103
C1
Tube.M-87-D3,2mmX3.5mm,AV31X2103
C2
Tubing.3/32"X5/32",S-50-HL AAX02004,Tygon(20feet)
C2
B-1
Appendices
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
T14
T15
T16
T17
T18
T19
T20
T21
T22
T23
T24
T25
T26
T27
T28
T29
T30
T31
T32
T33
T34
T35
T36
M90-100071--M90-100071--M90-100071--082-000108-00
082-000108-00
082-000108-00
M90-000025--082-000108-00
M90-100031--M90-100031--082-000108-00
082-000055-00
082-000055-00
082-000108-00
082-000108-00
082-000108-00
082-000055-00
M90-100031--M90-100031--M6G-020009--M6G-020034--0040-10-32301
3001-10-07069
Position
in the
Material description
Fluidic
diagram
Tubing.3/32"X5/32",S-50-HL AAX02004,Tygon(20feet)
C3
Tube.3/32"X5/32",S-50-HLAAX02004,Tygon
C3
Tube.3/32"X5/32",S-50-HLAAX02004,Tygon
C4
Tube.M-87-D3,2mmX3.5mm,AV31X2103
A1
B1
Tube.M-87-D3,2mmX3.5mm,AV31X2103
A4
Tube.M-87-D3,2mmX3.5mm,AV31X2103
A5
Tube.1/8"X1/4",R-3603 AAC02007,Tygon
A2
Tube.M-87-D3,2mmX3.5mm,AV31X2103
C2
Tube.PTFE,0.066"IDX0.098"OD
B2
Tube.PTFE,0.066"IDX0.098"OD
B5
Tube.M-87-D3,2mmX3.5mm,AV31X2103
A4
Tube.1/16"X3/16",F-5500-A,Fluran
A4
Tube.1/16"X3/16",F-5500-A,Fluran
Tube.M-87-D3,2mmX3.5mm,AV31X2103
B3
Tube.M-87-D3,2mmX3.5mm,AV31X2103
B3
Tube.M-87-D3,2mmX3.5mm,AV31X2103
B4
Tube.1/16"X3/16",F-5500-A,Fluran
A4
A3
Tube.PTFE,0.066"IDX0.098"OD
A3
Tube.PTFE,0.066"IDX0.098"OD
B3
Tubing. Silica gel 0.031"ID 0.156"OD
B3
3-way valve tubing(1.6*3.2
B3
Tube.PTFE,0.040"IDX0.066"OD
C3
Tube.1/16"X1/8",S-50-HLAAX02002,Tygon
71 Tube
T37
M90-100071---
Tube.3/32"X5/32",S-50-HLAAX02004,Tygon
72 Tube
T38
73 Tube
T39
74 Tube
75 Tube
T40
M90-100071---
Tube.3/32"X5/32",S-50-HLAAX02004,Tygon
T41
M90-100071---
Tube.3/32"X5/32",S-50-HLAAX02004,Tygon
C5
C5
T42
M90-100071--3001-10-07069
3001-10-07069
0040-10-32301
M90-100071--082-000055-00
M90-100031--M6G-020056--M6G-020056--3001-10-07069
3001-10-07069
082-000108-00
3001-10-07069
3001-10-07069
M90-000025--M90-100071--M90-100071--M90-100031--M90-100031--M6G-020006--M6G-020006---
Tube.3/32"X5/32",S-50-HLAAX02004,Tygon
C5
Tube.1/16"X1/8",S-50-HLAAX02002,Tygon
Tube.1/16"X1/8",S-50-HLAAX02002,Tygon
Tube.PTFE,0.040"IDX0.066"OD
Tube.3/32"X5/32",S-50-HLAAX02004,Tygon
Tube.1/16"X3/16",F-5500-A,Fluran
Tube.PTFE,0.066"IDX0.098"OD
Tube.EVA,ID:1/16＂,OD：1/8＂，clear
Tube.EVA,ID:1/16＂,OD：1/8＂，clear
Tube.1/16"X1/8",S-50-HLAAX02002,Tygon
Tube.1/16"X1/8",S-50-HLAAX02002,Tygon
Tube.M-87-D3,2mmX3.5mm,AV31X2103
Tube.1/16"X1/8",S-50-HLAAX02002,Tygon
Tube.1/16"X1/8",S-50-HLAAX02002,Tygon
Tube.1/8"X1/4",R-3603 AAC02007,Tygon
Tube.3/32"X5/32",S-50-HLAAX02004,Tygon
Tube.3/32"X5/32",S-50-HLAAX02004,Tygon
Tube.PTFE,0.066"IDX0.098"OD
Tube.PTFE,0.066"IDX0.098"OD
Tube.Silicone,1/16"X3/16",TYGON 3350
Tube.Silicone,1/16"X3/16",TYGON 3350
C4
D3
B2
C3
A4
A4
A4
A5
B5
B4
C2
B5
C5
C5
C2
C4
B3
B4
B3
B4
Material
ID
type
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Name in
the
diagram
Part NO.
C4
M90-100071---
Tube.3/32"X5/32",S-50-HLAAX02004,Tygon
C4
M90-100071---
Tube.3/32"X5/32",S-50-HLAAX02004,Tygon
C4
T43
T44
T45
T46
T47
T48
T49
T50
T51
T52
T53
T54
T55
T56
T57
T58
T59
T60
T61
T62
B-2
Appendices
ID
Material
type
97 Tube
98 Tube
99 Tube
100 Tube
101 Tube
Name in
the
diagram
T63
T64
T65
0040-10-32301
M90-100031--0040-10-32301
Tube.PTFE,0.040"IDX0.066"OD
Tube.PTFE,0.066"IDX0.098"OD
Tube.PTFE,0.040"IDX0.066"OD
T66
M90-000025---
Tube.1/8"X1/4",R-3603 AAC02007,Tygon
T67
T68
M90-100071--M90-000025---
Tube.3/32"X5/32",S-50-HLAAX02004,Tygon
Tube.1/8"X1/4",R-3603 AAC02007,Tygon
Part NO.
Material description
Position
in the
Fluidic
B3
B2
B2
C5
C5
102
103
104
105
106
107
108
109
110
111
112
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
Tube
T69
T70
T71
T72
T73
T74
T75
T76
T77
T78
M90-000025--M6G-020034--M6G-020034--M6G-020055--M90-000025--M6G-020055--M90-000025--M90-000025--M6G-020006--0040-10-32301
Tube.1/8"X1/4",R-3603 AAC02007,Tygon
3-way valve tubing(1.6*3.2)
3-way valve tubing(1.6*3.2)
Tube.TPU,ID：1/8＂,OD：1/4＂,clear
Tube.1/8"X1/4",R-3603 AAC02007,Tygon
Tube.TPU,ID：1/8＂,OD：1/4＂,clear
Tube.1/8"X1/4",R-3603 AAC02007,Tygon
Tube.1/8"X1/4",R-3603 AAC02007,Tygon
Tube.Silicone,1/16"X3/16",TYGON 3350
Tube.PTFE,0.040"IDX0.066"OD
D5
D4
C5
B5
B5
D5
D4
B3
A4
B2
B2
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
T79
Tube
Transit tube J1
Transit tube J2
Transit tube J3
Transit tube J4
Transit tube J5
Transit tube J6
Transit tube J7
Transit tube J8
Transit tube J9
Transit tube J10
Transit tube J11
Transit tube J12
Transit tube J13
Transit tube J14
Transit tube J15
Transit tube J16
Transit tube J17
Transit tube J18
Transit tube J19
Transit tube J20
Transit tube J21
Transit tube J22
Transit tube J23
Transit tube J24
Transit tube J25
Transit tube J26
Transit tube J27
Transit tube J28
Transit tube J29
Transit tube J30
Transit tube J31
M90-100071--M6G-020009--M6G-020009--082-000055-00
082-000055-00
082-000055-00
M6G-020006--082-000055-00
082-000055-00
082-000055-00
M6G-020009--M6G-020009--M6G-020009--0030-20-13339
082-000055-00
0030-20-13339
M90-100071--M6G-020006--M90-100071--M6G-020006--M6G-020009--M6G-020009--082-000055-00
0030-20-13339
M6G-020009--082-000055-00
0030-20-13339
082-000055-00
082-000055-00
082-000055-00
0030-20-13339
0030-20-13339
Tube.3/32"X5/32",S-50-HLAAX02004,Tygon
Tubing. Silica gel 0.031"ID 0.156"OD（1feet）
Tubing. Silica gel 0.031"ID 0.156"OD（1feet）
Tube.1/16"X3/16",F-5500-A,Fluran
Tube.1/16"X3/16",F-5500-A,Fluran
Tube.1/16"X3/16",F-5500-A,Fluran
Tube.Silicone,1/16"X3/16",TYGON 3350
Tube.1/16"X3/16",F-5500-A,Fluran
Tube.1/16"X3/16",F-5500-A,Fluran
Tube.1/16"X3/16",F-5500-A,Fluran
Tubing. Silica gel 0.031"ID 0.156"OD（1feet）
Tubing. Silica gel 0.031"ID 0.156"OD（1feet）
Tubing. Silica gel 0.031"ID 0.156"OD（1feet）
Transit tube(1.5mm-3mm)(Mould MR13339)
Tube.1/16"X3/16",F-5500-A,Fluran
Transit tube(1.5mm-3mm)(Mould MR13339)
Tube.3/32"X5/32",S-50-HLAAX02004,Tygon
Tube.Silicone,1/16"X3/16",TYGON 3350
Tube.3/32"X5/32",S-50-HLAAX02004,Tygon
Tube.Silicone,1/16"X3/16",TYGON 3350
Tubing. Silica gel 0.031"ID 0.156"OD（1feet）
Tubing. Silica gel 0.031"ID 0.156"OD（1feet）
Tube.1/16"X3/16",F-5500-A,Fluran
Transit tube(1.5mm-3mm)(MouldMR13339)
Tubing. Silica gel 0.031"ID 0.156"OD（1feet）
Tube.1/16"X3/16",F-5500-A,Fluran
Transit tube(1.5mm-3mm)(MouldMR13339)
Tube.1/16"X3/16",F-5500-A,Fluran
Tube.1/16"X3/16",F-5500-A,Fluran
Tube.1/16"X3/16",F-5500-A,Fluran
Transit tube(1.5mm-3mm)(MouldMR13339)
Transit tube(1.5mm-3mm)(MouldMR13339)
B2
D2
C2
C2
C2
C2
C2
A3
A3
B3
B3
B3
B2
B2
A4
A4
B3
B3
B4
B4
B2
B3
B2
B2
B2
B2
B2
C2
C2
A3
B2
B2
B-3
Appendices
ID
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
Material
type
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Connection
Tube
Transit tube
Transit tube
Transit tube
Tube
Tube
Name
in
the
diagr
am
C1
C2
C3
C4
C5
C6
C7
C8
C9
C10
C11
C12
C13
C14
C15
C16
C17
C18
C19
C20
C21
C22
C23
C24
C25
C26
C27
T80
J32
J33
J34
T81
T82
Part NO.
M90-100028--M90-100028--M90-100028--M90-100028--M90-100028--M90-100028--M90-100028--M90-100028--M90-100028--043-000880-00
M90-100028-03
M90-100026--M90-100026--043-000892-00
043-000892-00
M90-100027--M90-100027--082-001140-00
082-001141-00
082-001140-00
082-001141-00
M90-100009--M90-100025--M90-100009--M90-100025--M90-100100--M90-100100--0040-10-32301
0030-20-13339
082-000055-00
082-000055-00
M6G-020056--M6G-020056---
Material description
3/32 PE TEE FITTING-WHITE NYL T420-1
3/32 PE TEE FITTING-WHITE NYL T420-1
3/32 PE TEE FITTING-WHITE NYL T420-1
3/32 PE TEE FITTING-WHITE NYL T420-1
3/32 PE TEE FITTING-WHITE NYL T420-1
3/32 PE TEE FITTING-WHITE NYL T420-1
3/32 PE TEE FITTING-WHITE NYL T420-1
3/32 PE TEE FITTING-WHITE NYL T420-1
3/32 PE TEE FITTING-WHITE NYL T420-1
8.5mm three way connector
Connection.Y,400Barb,3/32"ID,White Nylon
Nylon plug (located at the reagent inlet)
Nylon plug (located at the reagent inlet)
straight tube tie-in
straight tube tie-in
Connection.Straight Through Reduction,1/8"&3/32"ID
Connection.Straight Through Reduction,1/8"&3/32"ID
Connection.Male Luer 2mm"ID natural PVDF
Connection.FemaleLuer,1/4-28UNF,1/8"ID
Connection.Male Luer 2mm"ID natural PVDF
Connection.FemaleLuer,1/4-28UNF,1/8"ID
Connection.FemaleLuer,1/4-28UNF,1/8"ID
Connection.Male Luer,1/8"Barb,White Nylon
Connection.FemaleLuer,1/4-28UNF,1/8"ID
Connection.Male Luer,1/8"Barb,White Nylon
Connection.Elbow,400Barb,3/32"ID,White,L420-1
Connection.Elbow,400Barb,3/32"ID,White,L420-1
Tube.PTFE,0.040"IDX0.066"OD
Transit tube(1.5mm-3mm)(Mould MR13339)
Tube.1/16"X3/16",F-5500-A,Fluran
Tube.1/16"X3/16",F-5500-A,Fluran
Tube.EVA,ID:1/16＂,OD：1/8＂，clear
Tube.EVA,ID:1/16＂,OD：1/8＂，clear
B-4
Position
in the
fluidic
diagram
C1
C1
C1
C1
C3
B5
C5
C5
C4
B3
B3
B3
B4
A4
A4
C5
A5
B5
B5
B5
B5
A5
A5
B4
B4
C2
A2
A4
A4
C2
C2
B5
A5
C.
Hardware block diagram
C-1
D.
Cables and Wires
Material ID
009-002857-00
Name
Heater connecting wire
009-002858-00
Temperature sensor
connecting wire
Indicator board connecting
wire
Analog board supervisory
signal wire
Optical signal output wire
Analog main control board
SPI signal wire
Optical control signal wire
Syringe motor connecting
wire
Digital power connecting
wire
Analog power connecting
wire
Sampling motor connecting
wire
Electromagnetic metering
pump connecting wire
Valve connecting wire
Waste pump connecting
wire
Float switch connecting
wire
Sampling photocoupler
connecting wire
Touchscreen control cable
009-002863-00
009-002865-00
009-002866-00
009-002867-00
009-002869-00
009-002870-00
009-002871-00
009-002872-00
009-002875-00
009-002877-00
009-002878-00
009-002880-00
009-002881-00
009-002882-00
009-002911-00
009-002913-00
009-002967-00
009-002988-00
009-002989-00
009-003176-00
009-003322-00
009-003381-00
009-003642-00
2800-20-28762
009-004057-00
LCD signal wire
RBC connecting wire
Safety ground
Component ground
Syringe assembly
photocoupler connecting
wire
Start key connecting wire
Fan adapter cable
Hydraulic sensor adapter
cable
HGB connecting wire
LH&DIFF detect board wire
Connection
Connects A-J21 with heater and
temperature switch
Connects A-J10 with temperature
sensor
Connects B-J78 with indicator board
Connects A-J2 and B-J86
Connects A-J4 and B-J85
Connects A-J31 and B-J81
Connects A-J3 and B-J77
Connects A-J32 and syringe motor
Connects C-J1/C-J2 and A-J22/B-J68
Connects A-J8 and C-J3 C-J4
Connects A-J24 and sampling motor
Connects A-J16 and electromagnetic
metering pump
Connects A-J14 A-J15 and valves
Connects A-J20 and waste pump
Connects A-J1 and float switch
Connects A-J12 and sampling
photocoupler
Connects B-J67 and touchscreen drive
board
Connects B-J4/B-J16 and LCD/backlight
Connects A-J6 and RBC bath
/
/
Connects A-J13 and syringe assembly
photocoupler
Connects B-J99 and start key
Connects A-J17 A-J18 and fan
Connects A-J11 and hydraulic sensor
Connects A-J5 and HGB bath
Connects A-J26 and Liquid detect
board PCBA
Note: A refers to the analog drive board, B refers to the main control board, C refers to the power
board
E.
No.
Appendix Table
Product Model
Inspection Item
Description
1
Electrical connection
Power cable
connection
2
Indicator color
Color
3
Indicator sound
Sound
4
Touchscreen
Touchscreen
5
Time and date
Enter time and date
6
Version
7
Syringe and sampling
mechanism self-test
8
Valves self-test
9
Fan self-test
10
Mechanical position
adjustment
11
Mechanical position
adjustment
12
Pressure verification
13
Temperature
calibration
14
Temperature
calibration
15
Temperature
calibration
16
17
Counting channel
measurement
Counting channel
measurement
Counting channel
measurement
18
19
20
21
Counting channel
measurement
Counting channel
measurement
Counting channel
measurement
SERIAL NUMBER
Requirement
Refer to related
graphic files in
the appendix
Red indicates
problems, green
indicates OK
Alarms when
problems exist
Touchscreen
calibration
completed
Correct time and
date entry
Result
Conclusion
□OK □NG
□PASS □FAIL
□OK □NG
□PASS □FAIL
□OK □NG
□PASS □FAIL
□OK □NG
□PASS □FAIL
□OK □NG
□PASS □FAIL
In latest versions
□OK □NG
□PASS □FAIL
Normal operation
□OK □NG
□PASS □FAIL
Valves
Normal operation
□OK □NG
□PASS □FAIL
Fan
Normal operation
□OK □NG
□PASS □FAIL
Refer to
Mechanical
position
adjustment
□OK □NG
□PASS □FAIL
Refer to
Mechanical
position
adjustment
□OK □NG
□PASS □FAIL
□OK □NG
□PASS □FAIL
Version and
configuration
information
Syringe and
sampling
mechanism self-test
Adjustment of the
relative position
between the
sample probe and
the WBC bath
Adjustment of the
relative position
between the
sample probe and
the RBC bath
Vacuum
Preheat bath
temperature
calibration
Preheat bath
temperature
calibration
Preheat bath
temperature
calibration
Bubbles in sample
tube or not
Fluid residue on the
sample probe
Sample probe
below the fluid level
and clear from the
reaction bath
Bubbles in sample
supply tube or not
Bubbles in WBC
bath or not
Bubbles in RBC bath
or not
Pressure within
range
Machine
measurement
value
□PASS □FAIL
FRU value
□PASS □FAIL
Overall difference
□PASS □FAIL
No bubbles
□OK □NG
□PASS □FAIL
No residue
□OK □NG
□PASS □FAIL
Normal down
position
□OK □NG
□PASS □FAIL
No bubbles
□OK □NG
□PASS □FAIL
Bubbles in bath
□OK □NG
□PASS □FAIL
Bubbles in bath
□OK □NG
□PASS □FAIL
E-1
Connection and Tube
22
Counting channel
measurement
Counting channel
measurement
23
Counting channel
measurement
24
25
Counting channel
measurement
26
Counting channel
measurement
Aperture voltage
splash when WBC
bubbling or not,
bubbles on filling
tube or not
splash when RBC
bubbling or not,
bubbles on filling
tube or not
WBC bath can be
drained correctly or
not
RBC bath can be
drained correctly or
not
[16, 21]V
□PASS □FAIL
No splash and no
contact
□OK □NG
□PASS □FAIL
No splash and no
contact
□OK □NG
□PASS □FAIL
Drained correctly
□OK □NG
□PASS □FAIL
Drained correctly
□OK □NG
□PASS □FAIL
WBC bath wall
No residue
□OK □NG
□PASS □FAIL
RBC bath wall
No residue
□OK □NG
□PASS □FAIL
29
Counting channel
measurement
Counting channel
measurement
Counting channel
measurement
RBC bath shield box
installation
Screws tightened
□OK □NG
□PASS □FAIL
30
Counting channel
measurement
Sample probe
movement
□OK □NG
□PASS □FAIL
31
Counting channel
measurement
Sample probe tube
□OK □NG
□PASS □FAIL
32
Maintenance
Perform
maintenance and
cleaning
Refer to the user's
manual
□OK □NG
□PASS □FAIL
RBC aperture
voltage
[16, 21]V
□PASS □FAIL
RBC
≤0.02×1012/L
□PASS □FAIL
HGB
≤1g/L
□PASS □FAIL
HCT
≤0.5%
□PASS □FAIL
PLT
≤10×109/L
□PASS □FAIL
27
28
Sample probe
moves with no
interference
Sample probe
tube does not
interfere with
other structures
37
Reaction bath voltage
measurement and
setup
Analyzer status
verification - count
Analyzer status
verification - count
Analyzer status
verification - count
Analyzer status
verification - count
38
Optical system tube
connection
Connect the optical
tubes and fill with
fluids
Refer to the
service
instructions
39
Analyzer voltage
Power (+12V)
[11.5, 12.5]V
□PASS □FAIL
Analyzer voltage
Power (+24V)
[22.0, 29.0]V
□PASS □FAIL
Analyzer voltage
Analog (+12V)
[11.5, 12.5]V
□PASS □FAIL
Analyzer voltage
Constant current
source voltage
(directly measured)
[47.0, 60.0]V
□PASS □FAIL
Analyzer voltage
LAS blank voltage
[0, 0.5]V
□PASS □FAIL
44
Analyzer current
Laser current
[20, 70]mA
□PASS □FAIL
45
Background Count
WBC
≤0.2×109/L
□PASS □FAIL
46
Background Count
RBC
≤0.02×1012/L
□PASS □FAIL
Background Count
HGB
≤1g/L
□PASS □FAIL
33
34
35
36
40
41
42
43
47
E-2
□OK □NG
□PASS □FAIL
Connection and Tube
48
49
50
51
52
53
54
55
56
57
58
Background Count
HCT
≤0.5%
□PASS □FAIL
Background Count
PLT
≤10×109/L
□PASS □FAIL
Standard particle
graphic parameter test
Standard particle
graphic parameter test
Standard particle
graphic parameter test
Standard particle
graphic parameter test
Standard particle
graphic parameter test
Standard particle
graphic parameter test
Standard particle
graphic parameter test
Standard particle
graphic parameter test
Standard particle
graphic parameter test
Optical gain
Total LAS
1500~3000
LAS CG Position
38~42
LAS CV
≤6.50%
Total MAS
1500~3000
MAS CG Position
107~158
MAS CV
≤3.0%
Total WAS
1500~3000
WAS CG Position
135~220
WAS CV
≤8.0%
59
Optical gain
60
Optical gain
61
Optical gain
62
Impedance gain
63
HGB gain
64
65
Shutdown
LAS
MAS
WAS
Width
RBC
HGB
Shutdown process
N/A: Not
applicable
N/A: Not
applicable
N/A: Not
applicable
N/A: Not
applicable
N/A: Not
applicable
N/A: Not
applicable
No failure or
alarm occurs
Tested by:
E-3
□PASS □FAIL
□PASS □FAIL
□PASS □FAIL
□PASS □FAIL
□PASS □FAIL
□PASS □FAIL
□PASS □FAIL
□PASS □FAIL
□PASS □FAIL
□PASS □FAIL
□PASS □FAIL
□PASS □FAIL
□PASS □FAIL
□PASS □FAIL
□PASS □FAIL
□OK □NG
Date:
□PASS □FAIL
P/N: 046-008515-00(3.0)