GENETICS REEBOB Activity Objective: To explore genetic variation in a species by constructing fictitious baby “Reebops.” Students will randomly receive the genotype of a “reebop” and construct the “reebop” according to its genes. Pre-Lab: Terms to be defined for this lab homologous pair gametes sperm egg/ovum zygote haploid diploid meiosis genetic variation phenotype genotype Materials: magic markers Antenna Pretzel sticks Tail Red, blue, or green sour straws Humps on back Green mini marshmallows connected with half toothpicks Yellow, orange, or pink mini marshmallows connected with half toothpicks Nose color Legs (number) Eyes Body Segments and Head Green, blue, or brown jelly beans connected with half toothpicks Large white marshmallows connected with toothpicks Procedure: 1. Divide into groups of 2. If there are an odd number of students, you will have to have one group of 3. 2. Each group will receive an envelope containing one set of parent chromosomes. One person from each group should take responsibility for the chromosomes. 3. One color of chromosomes is from the mother and the other is from the father. Separate the chromosomes into two separate piles according to their color. On your answer sheet, designate which chromosomes belong to which parent before you start. (ie. Mother’s Chromosomes = purple, Father’s Chromosomes = red) 4. Look at the 14 chromosomes in the Mother pile. Match up the homologous pairs. A homologous pair will be the same length. You should have 7 pairs when you are done sorting them out. Lay the mother’s homologous pairs in a row, face down to the left of your lab table. 5. Repeat step 4 for the father’s chromosomes and place them to the right of your lab table. 6. Look at the largest homologous pair of the mother. Choose ONE of the two. Place the rejected one back in the envelope. 7. Look at the largest homologous pair of the father. Choose ONE of the two. Place the rejected one back in the envelope. 8. Repeat steps 6-7 for the other 6 homologous pairs of the mother and father. 9. You should have 7 chromosomes from the mother and 7 from the father. Match up the homologous pairs- in each pair one should be from the mother and one should be from the father. 10. On your answer sheet, write down the 7 gene pairs (genotypes) that were selected in Table 1. 11. Next write down the phenotypes (physical traits or features) that your baby reebop will have. Use the genotypes and phenotypes below in the “Reebop Genetic Decoder”. 12. Based on your baby reebop’s traits, make a ‘shopping list’ of the parts you’ll need to build your baby by filling in Table 1 on your answer sheet. All baby reebops have a head so the minimum number of marshmallows you need is two ( one body segment & a head). Also, be sure you’ve accounted for the total number of toothpicks you will needthey will be used to connect body parts together AND used for the legs. Once you have checked your list thoroughly, place all of the chromosomes back in the envelope. 13. One person from each group should bring the envelope with ALL of the chromosomes and the shopping list to the Parts Manager (teacher!) to collect your reebop parts. (DO NOT EAT ANY PART OF YOUR CHILD OR POINTS WILL BE DEDUCTED FROM YOUR LAB). 14. Using the list you made and the parts you’ve collected, construct your baby reebop. Once you’ve finished, select a name for your baby and write it on a sheet of paper, along with the names of the members in your group. Take your baby reebop and its name tag to your teacher to turn in for a grade. Reebop Genetic Decoder: AA= 1 antenna Aa = 2 antenna aa = no antenna MM = 1 green back hump Mm = 2 green back humps mm = 3 green back humps QQ = yellow nose Qq = orange nose qq = pink nose EE = 2 green eyes Ee = 2 blue eyes ee = 2 brown eyes LL or Ll = 2 legs per body segment ll = 3 legs per body segment TT = red tail Tt = blue tail tt = green tail DD or Dd = 1 body segment dd = 2 body segments Name(s)___________________________________________________________ Period ________________ Genotype Phenotype Materials Needed 1 2 3 4 5 6 7 Qty ntity Total # of toothpicks needed: ________________________________________ 1. Which traits were heterozygous? What was the phenotype? 2. Which traits were homozygous dominant? 3. Which traits were homozygous recessive? What was the phenotype? 4. Why weren’t all of the marshmallow creatures in class identical? Explain in terms of the meiotic process. 5. If two babies both had red tails, would their genotypes for that trait have to be identical? Show the possible genotypes. 6. If two babies both had an orange nose and one green hump, would their genotype have to be identical for these traits? Show the possible genotypes. 7. If the chromosomes that determine gender had been a part of this activity, which allele (X or Y) would have determined the gender of the baby? 8. Did you have any genes which were an example of incomplete dominance? Explain. 9. Which trait involves multiple alleles, and what are the alleles involved? 10. Which trait, if any, was an example of co-dominance? How does this differ from incomplete dominance? 11. What genetic pattern did the gene for nose color illustrate? Explain. 12. Draw a monohybrid cross Punnet square for the trait of tail type (for this example, mom is heterozygous and dad is homozygous dominant) and predict the probability of getting a baby which displays your baby’s phenotype for this trait. 13. Which traits were homozygous recessive? What was the phenotype? 14. Why weren’t all of the marshmallow creatures in class identical? Explain in terms of the meiotic process. 15. If two babies both had red tails, would their genotypes for that trait have to be identical? Show the possible genotypes. 16. If the chromosomes that determine gender had been a part of this activity, which allele (X or Y) would have determined the gender of the baby? 17. What genetic pattern did the gene for nose color illustrate? Explain. 18. Draw a monohybrid cross Punnet square for the trait of tail type (for this example, mom is heterozygous and dad is homozygous dominant) and predict the probability of getting a baby which displays your baby’s phenotype for this trait. A a M m T E e L Q q t l D d A a M m T E e L Q q t l D d