ATLAS (PHOTOS) MALE REPRODUCTIVE SYSTEM -consists of gonads/testes, excretory ducts, accessory reproductive glands and penis Testis- subdivided by a connective tissue septa into lobules containing seminiferous tubules Seminiferous tubule- lined by the stratified germinal epithelium, consisting of two major cell types; spermatogenic cells & non proliferating supporting cells (sertoli cells) Spermatogenic cells- divide, differentiate and produce spermatozoa by a process called spermatogenesis. Interstitial cells of Leydig- epithelial connective tissue Germinal epithelium- specialized stratified epithelium that lines the seminiferous tubules SPERMATOGENESIS (1) Mitotic divisions of spermatogonia to from spermatocytes (2) Two meiotic divisions in which the number of chromosomes is reduced by half; spermatids (3) Spermiogenesis- morphological transformation into spermatozoa Blood-testis barrier- prevents the entrance of harmful substances from blood into the germinal epithelium SERTOLI CELLS (1) Support, protect and nutrition of the developing sperm cells (2) Phagocytosis of the excess cytoplasm of the developing spermatids (3) Release of spermatozoa into seminiferous tubules (4) Secretion of testicular fluid as transport medium (5) Production of androgen-binding protein and inhibin Follicle stimulating hormone- controls the androgen binding production of sertoli cells Androgen-binding protein- increases the concentration of testosterone in the seminiferous tubules for proper spermatogenesis Inhibin- hormone that suppresses/inhibits the production of FSH Interstitial cells of Leydig- clusters of epithelioid cells between the seminiferous tubules; produces testosterone Testosterone- hormone necessary for spermatogenesis; depends primarily on Luteinizing hormone Luteinizing hormone-stimulates the production of testosterone by interstitial cells *Testosterone- need to be high levels for spermatogenesis TESTIS VIEW (ORDER) Tunica albuginea-thick connective tissue Tunica vasculosa- vascular layer of loose connective tissue Interstitial connective tissue- connective tissue; surrounds, binds and supports the: seminiferous tubules Septa- thin and fibrous; divides the testis into compartments called lobules FEMALE REPRODUCTIVE SYSTEM -consists of the ovaries, uterine tubes, uterus, vagina, mammary glands and the external genitalia. Follicle stimulating hormone- influences the growth and maturation of ovarian follicles and stimulates the granulosa and thecal cells of the maturing follicles to produce estrogen. Luteinizing hormone-stimulates the lutein cells to secrete estrogen and large amounts of progesterone; induces rapid transformation of the granulosa and theca interna cells OVARY VIEW (ORDER) Germinal epithelium- low cuboidal or squamous cell that covered the ovarian surface Tunica albuginea- connective tissue layer Cortex- occupied greater part of the ovary Medulla- dense, irregular connective tissue SCHOENWOLF LAB GUIDE Oogenesis and Fertilization Oogenesis- begins in the paired ovaries Follicles contain a large central cell containing yolk in its cytoplasm - PRIMARY OOCYTE Follicle cells-surrounds the primary oocyte Germinal vesicle- large nucleus in the primary oocyte Vitelline membrane- lies between the follicle cells and the plasmalemma Theca folliculi externa- forms the surface layer of the ovary Theca folliculi interna- partially surrounds each follicle *Each ovary contains cells called oogonia Oogonia- undergo rapid mitotic divisions *Primary oocytes enter the prophase of the first meiotic division forming the first polar body and secondary oocyte. *secondary meiotic division is initiated by the secondary oocyte *Fertilization occurs externally (frogs) *second meiotic division is completed only when a sperm contacts and penetrates the secondary oocyte, resulting into a second polar body and a mature ovum Gastrulation Involution- cells located on the surface turn inward ● Germ layers ENDODERMMESODERM ECTODERM Blastopore+dorsal blastoporal lip= gastrula Yolk plug- yolk filled endodermal cells Exogastrulation- process which surface cells move but fail to involute over the blastoporal lips Cleavage and Blastulation Cleavage- consists of series of rapid mitotic divisions that result in blastulation (formation of blastula) Blastomeres- group of cells surrounding a cavity (blastocoel) Cleavage is total (holoblastic) *furrows that are pass through the vegetal hemisphere is slow than the animal hemisphere (more yolk in the animal hemisphere) Micromeres- 4 small animal hemisphere cells Macromeres- 4 larger vegetal hemisphere cells Animal hemisphere- heavily pigmented cortex; 4 or 5 cells thick; blastomeres contain very little yolk Vegetal hemisphere- a pigmented cortex; blastomeres are very large in few numbers; blastomeres are packed w yolk Neurulation Neural Ectoderm- induced to thicken as the neural plate *from gastrula to neurula