Meiosis and Mitosis Lab Overview: Unique Diploid (2x) 2 Identical 4 Mitosis is the division of a ____diploid_________ cell to produce _2___ new _____identical_____ cells. Meiosis is also the division of a ____diploid____cell. However, it produces __4____ new, _____Unique______cells. Cell cycle: 1. Define the term cell cycle? The cell cycle is the process of cells 2. What is the meaning of genome? Compare your genome to that of a prokaryotic cell. 3. Give 3 reasons why a mitotic cell division is important? a) b) c) 4. Give a brief explanation of what happens in each part of the cell cycle as indicated. G1 Interphase S Mitosis G2 Cytokinesis 5. List the steps and images of Mitosis in the correct order and mention a major event that occurs in each stage. 6. Why is it important for multi-cellular organisms to regulate Mitosis? Why would it be bad for cells to divide at any time? 7. How does cytokinesis differ in plants and animals? The following table represents the number & percent of cells counted for each phase of mitosis. 8. Calculate the percentage of cells in each phase of mitosis. Phase Number of Cells Interphase 46 Prophase 23 Metaphase 15 Anaphase 12 Telophase 14 Percent Cells 9. In which phase do cells spend the most time? Use the data above to support your answer. Cytokinesis 10 10. Why do cells spend a significant portion of Mitosis in prophase? 11. List the 6 steps of cell division in order and briefly describe the events of each. Technically, which step marks the end of mitosis? 12. How many chromosomes are present in a human cell at the beginning of prophase? ______ Chromatids? ______ 13. What role do cyclin and CDK play in regulating cell division? 14. What happens to cyclin levels as the cell passes through the S phase, the G2 phase and enters mitosis? 15. What happens to cyclin levels between mid-mitosis and the G1 phase? 16. List the 3 checkpoints cells must pass through in order to carry out the phases of the cell cycle. For each checkpoint, list the tasks and/or control mechanisms that allow the cell to proceed to the next step in the cell cycle. I. II. III. Meiosis No Mitosis Duplication +/tn Spore cells of a certain mold known as Sordaria can be viewed under a microscope. Like most fungi, Sordaria spends the majority of its life in the haploid stage. Some Sordaria produce black spores (+) and some produce tan spores (tn) which are packaged in a pod-like structure called an ascus. If one parent is homozygous black (++) and is crossed with a homozygous tan parent (tn/tn), the hybrid will have the genotype ______ as one parent would donate the + allele and the other would donate the tn allele. During Meiosis I, these chromosomes first undergo ________________ in which two sister chromatids are formed. During Meiosis II, the sister chromatids split and end up in separate cells. The next phase in the formation of spores is that each haploid cell undergoes ______________ to produce two identical haploid cells (2 black or 2 tan). When 4 black spores or 4 brown spores appear in a row, it is assumed that ________ crossing over occurred during Meiosis I. **According to this diagram, no crossing over occurred during Meiosis I between the tan (tn) and black (+) genes. 1. Why would the chromosomes carrying the black allele (+) and the tan allele (tn) be considered homologous chromosomes? 2. Assume that a cell has the following 4 alleles: AaBb located on 4 separate chromosomes. Using these four alleles, sketch the following phases of meiosis. Place each letter on a separate chromosome. The first has been completed for you. Prophase I Anaphase I Cytokinesis I Homologous chromosomes pair up and may cross over…no crossing over is shown here Anaphase II Cytokinesis II v 3. List four differences between meiosis and mitosis. Mitosis Meiosis