Advance Cultivation Practices of basil (Ocimum sp.) Vipin Kumar1, RK Srivastava2 1 Technology and Business dissemination, CSIR– Central Institute of Medicinal and Aromatic Plants (CIMAP), Picnic Spot Road Khurram Nagar Lucknow- 226015,vipinkumar210428@gmail.com, 2 rksrivastava@cimap.res.in Abstract The ocimum genus is a most economically important medicinal and aromatic herb collectively called basil is a perennial, herbaceous and erect shrub. Basil is native to Asia and can be observed growing in tropical to sub-tropical regions of the world. Essential oil obtained from leaves and flowering top of the plants are used in food, flavours, perfumery and pharmaceutical industries. Use and production of basil has been carried out from ancestors but is commercially cultivated for its valuable medicinal and economically important essential oil purposes from last few decades in different part of the word. The present chapter deals its historical background, distribution, uses, species and varieties, agro-technology for its cultivation, diseases and their management and processing of oil. Keywords: Ocimum, Medicinal and aromatic plants, Essential oil, Cultivation, eugenol Introduction Ocimum sp. is commonly known as Basil in English and Tulsiin Sanskrit meaning ‘matchless one’. Common name of basil is derived from Greek word “besileus” which means“king” and the botanical name of ocimum derived from Greek word “Ozo” which means“fragrant”. Due tobasil popularity and uses, the plant often refers as “queen of herbs” (Joshi 2017).In different locations of the world, Ocimum basilicum is known by different name. In india, in hindi and Bengali it is known as Babui Tulsi. In various other countries, it its’ common name is, basilica, basilikum or basilienkraut, basilica, rehan and albahaca are known in French, German, Italian, Arabic and Spanish respectively (Kirtikar and Basu, 2003, Bilal et al., 2012). Ocimum sanctum (holy basil) is predominately valued in indian hindu custom. It is profoundly helpful and remedial spices used as flavor and delicacy,grownin most of the differentareas of India.Ocimum sanctum L. is grown in temple, household precinct. Basically ocimum is one of the most importantmedicinal plant asit has tremendous medicinal properties beside it has a good quantity of essential oil in different plant partsso it also falls in aromatic plants categories, The plant isalso grows in pot in the Indian houses as an ornamental plant for its attractive look, beauties and fragrance (Paton et.al. 1999). The medicinal properties of Tulsi have attributed not only in Ayurvedic and Siddha but also in Greek, Roman and Unani systems of medicine (Rajanet. al. 2005).In Ayurveda, the use of tulsiis described as sovereign sinceancestralperiodand isdocumented as ‘elixir of life’ and isclaimed for longevity (Puri 2002).In traditional system of Indian medicine, thedifferent plant part of basil viz. leaves, seed, flowers, stem, root even whole plant are used to treat severe ailments such as fever, cough and cold, malaria, asthma, diarrhea, skin disease etc. (Prakash and Gupta 2005).Globally Ocimum is considered an important economic medicinal and aromatic group of plants, widely distributed in tropical, subtropical and warm temperate regions of the world (Paton et.al. 1999, Kumar et. al. 2018). Due to antioxidant and anti-aging properties of Tulsi, Hindus use fresh leaves in the panchamrut or charanamrut drink after Pooja. Ocimum genus contains about 150-160 species and varieties which are native to central Asia and south Africa (Ghosh 1995, Pushpangadan and Bradu 1995). Ocimum is annual/perennial, herbaceous, erect, woodyplant withmultiple branches, belongs to the family Lamiaceae (Mukherjee and Dutta 2007, Hereman S. 1868). Due to the presence of incredible medicinal and aromatic properties basil have received attention of the researchers in last few yearssignificantly (Makri, O 2007, Simon, J.E. 1990, Baczek, K. 2019). The major important metabolites found in essential oil of ocimum species include methyl chavicol, methyl cinnamate, eugenol, methyl eugenol, linalool, citral, camphor, geraniol, linalyl, thymol,safrol, taxol, ursolic acid (Upadhyay et.al. 2015, Gupta et.al. 2018) . Area under cultivation and its production In india it is cultivate about (…) ha and production is (…..). Jammu and Kashmir is major produce state in india. The highest productivity is 150 kg/ha oil and 250 q/ha herb in Jammu & Kashmir. The total production of Ocimum in the word about (…..) and the major producer country is (---). There is very good scope for its cultivation and global basil essential oil market size will grow by USD 186.5 million during 2019-2023, progressing at a CAGR of almost 8% during the forecast period(Business wire). North America is the largest region of Basil Leaves in the world in the past few years. Asia Pacific market took up 37.87% the global market in 2019, while Europe and North America were about 4.35%, 51.23%. McCormick, Litehouse, Fresh Origins, Frontier Natural Products, Herbs Egypt, etc. are the key suppliers in the global Basil Leaves market. The global Basil Leaves market size is about US$ 62 million by 2026, from US$ 57 million in 2020, at a CAGR of 1.3% during 2021-2026(Source: Absolute Reports Pvt Ltd July 16, 2020). General History There are many theories about the origin of basil but itis believed that ocimum has origin fromindia. The historical literature evidencedthat tulsi was first depicted in 4000-5000 B.C. in Rigveda where it wasaccepted to be the "Sovereign of Herbs".In Sanskrit Tulsi is described as "matchless one". In mythological texts, several stories existed about origin of Tulsi but common andpopularly is associated with treachery by lord Vishnu, Tulsi was mislead to betray her husband when lord Vishnu in disguise of her husband and seduced her. In immense pain, she committed suicide, and in her response lord Vishnu affirmed that all women would worship her for her faithfulness and her blessing would prevent women from becoming widows (Gupta 1971, Shah et.al. 2018). Therefore, holy basil is worshiped by Hindus as a goddess (Gupta 1971).Many believe that Ocimum came to India from England during 1500BC and arrivedin USA in 1600BC (Darrah 1980). Distribution Ocimum species are used as a herbs and basil is distributed in India, Africa, Arabia, Australia, Sri Lanka, Bangladesh, Thailand, China, Myanmar, Malaysia and can be noticedgrowing wildly in tropical to subtropical regions of the world.Ocimum contain about 150-160 species (Ghosh 1995, Pushpangadan and Bradu 1995) in whichnine species of ocimum from india (Balyan and Pushpangadan 1988). The seven spices of ocimum include Ocimum basilicum, Ocimum canum, Ocimum filamentosum Forssk, Ocimum americanum L., Ocimum afrcanum Lour, Ocimum kilimandscharicum Gurke, Ocimum tenuiflorum L. and Ocimum gratissimumare currently recognized fromIndia.The species Ocimum minimum could not bejustified and was suggested a subspecies or even a variety of Ocimum basilicum. In India, Ocimum is commonly cultivated in Uttar Pradesh, Madhya Pradesh, Jammu Kashmir, Tamil Nadu which varies fromplains to hills uptothe altitude of 1800-2000 m in Himalayas. Uses The uses of basil are comprehensive worldwidedue toitshuge properties asmedicinal, aromatic, spices and ornamental. The uses of basil in Ayurvedic medicine is a log history. Not only in Ayurveda but also siddha system of medicine, basil uses for prevention and cure of many illnesses is mentioned (Govindarajan et.al. 2005). The Ayurvedic system of Indian medicine utilizes different part of Tulsi viz. root, stem, leaves, flower, seed and even whole plant use in fever, malaria, asthma, diarrhea, skin disease, bronchial, eye disease, bronchitis and insect bite (Prakash and Gupta 2005). The plant is also useful in the treatment of stomach pains, fever, cough, worms and gout. The leaf juice is used as a nasal douche, a nostrum for ear ache and which is given with honey. For centuries, the dry leaves of Tulsiare beingmixed stored grains to repel insects (Biswas and Biswas 2005).The English people used basil as food flavoring and insecticide in 1600s (Gille et.al. 2007). The seeds of tulsi are refreshing and have sedative properties. Tulsi seeds are taken with water, juice or cow’s milk as an antioxidant, nourishing, mucilaginous and demulcent.In aromatherapy, ocimum is usedtocontrol cold, cough, bronchitis, sinusitis, fever, flue, depression, nervous tension etc. (Srivastava et.al. 2009). Sweet basil (Ocimum basilicum) is used as food flavoring, perfumery and cosmetics industries. The essential oil of tulsiis also used in soaps, cream, food and flavours. The point of view Ocimum contain various economically and industrially important essential oil rich in eugenol, methyl chavicol, safrol, linlool, camphor, citral, geraniol, linalyl acetate, methyl cinnamate that can harnessed to producenew commercial products(Grayer et.al. 1996 and nacar et al. 2000). Basil leaves are used in addition with other herbs, food and flavors like garlic, mustard, sage paprika, pepper in soups, teas, rice, drink and vegetable.Jewish folklore implies that duringfasting, basil gives you strength (Miele et. al. 2001). Botany The genus Ocimum contains about 160 species which are originated in the world. Various species of Ocimum vary in growth habits, colour, fragrant, flavours and uses composition, making the true botanical identity of basil difficult.Basil belongs to the Ocimum genus and family Lamiaceae, identified for their medicinal and aromatic significance.It is an annual or perennial, herbaceous or under shrubs, erect, woodywhich attains height of 80-120 cm. The stem of the plant is branched, obtusely quadrangular and bear leaves. The leaves of the plants are petiolate, opposite, margin entire or serrate, dark green or yellowish green in colour, width about 5 cm long and 2 cm wide. The inflorescence is terminal, simple, branched, cymesessile and under branched. The flowers of are small, bracteolate,creamy white in colour. The calyxremain bilabiate, ovoid with the upper lobe rounded and spreading, stalked and peculiarly smooth, soft and cool in touch. Corolla is bilabiate, declinate withshort tube. Stamens remain four which are didynamous, declinate, exserted, filaments free. Disk is entire three or four lobed andovary is glabrous, style declinate, subulate and flattened. Nutlets are obovoid and oblong(Suddee et.al. (2005; Kumar et. al. 2018). Species of Ocimum: The important species of Tulsi viz. Ocimum sanctum, Ocimum basilicum, Ocimum canum, Ocimum americanum, Ocimum afrcanum, Ocimum citriodorum, Ocimum kilimandscharicum and Ocimum gratissimum are under cultivaton for medicinal and aromatic purposes(Chopra et. al. 1956, Sen 1993; Gupta et. al. 2002). Sweet basil or French basil (Ocimum basilicum)is a most important commercial cultivar available in the market (Darrah 1974).Ocimum basilicumis majorly being grown for its green leaves and economically important essential oil whichis mainly extractedfromits leaf, used in cosmetic, perfumery and pharmaceutical industries (Prakash and Gupta 2005).Methyl chavicol and linalool are the major chemical compounds in Ocimum basilicum (Hiltunen and Holm 1999:91). Ocimum basilicumnative to India and Asia,is an annual, green herb, branchedstem, white labiate flower, about one meter in height and.The essential oil is extracted from leaves and flowering top through distillation process. Due to greenness and attractive lookof plant isalso grown as an ornamental plant (Moraleset. al. 1996, Darrah, H.H. et. al.1974). Ocimum sanctumor Ocimum tenuiflorumknown as holy basil, the word ‘holy basil’ comes from its use and importance in Hindus. The Sanskrit name is tulsi (Monier-Williams 1899:451),commonly found as Black (Krishna Tulsi) and green (Rama Tulsi)colour and are grown for its plant parts used in medicinal purpose. It is well known sacred plant. It is much branched, erect herb 60 o 90 cm in height, leaves are ovate - lanceolate, acuminate opposite about 1.0 to 3.0 cm long margin with entire or toothed dotted with minute oil glands.Flowers are small purple or white present in small compact clusters on spikes.Fruits are small having yellowish or reddish color (Baser and jain 2016). Ocimum killimandscharicum is an economically important perennial herb/ under shrub up to 1 m tall with woody stem, small flower, widely used in colds, coughs, abdominal pains, diarrhea and measles. It is also a richest source of essential oil extracted from leaf which used as insect repellents, storage pest, antibacterial and antioxidant activity (kokwaro etal. 1976, prasad etal. 1986, khakkim et al. 2008) Brush basil (Ocimum minimum) is annual, bushy, much smaller plant withovate leaves andwhite flower. Generally brush basil ispropagated through seed and flower in July to august (Martins et al. 1999). Ocimum viride also known as ‘fever plant’, is native of western Africa. The leaves are used in preparation of decoction drunk as a remedy for the fevers. Calaminthaclinopodium, known as wild basil or hedge basil orhedge calamint. Wild basil is an erect plant andstem thickly covered with soft hairs (Grieveet. al. 1999, Pushpangadanet. al. 1995). Common Name 1. Holy basil Ocimum sanctum 9 2. Sweet basil Ocimum basilicum 48 Methyl (40-50) 0.5-2 chavicol (25-30) Herb 3. Bush or Greek Ocimum canum basil Hoary basil Ocimum americanum 24 26 72 Linalool camphor Methyl (70-75) Herb 4. Botanical Name Chr. No. Major chemical constituents % Eugenol (60-75) S.N. Oil (%) 0.5-1.0 2-4 2.5-5 chavicol 3.6-6.5 Habit Herb Herb 5. - Ocimum citriodorum - Citral (65-70) 1.5-2.5 Herb 6. Camphor basil Ocimum kilimandscharicu m 76 Camphor (70-80) 1.5-3.5 Shrub 7. Shrubby basil Ocimum gratissimum 10 Eugenol (70-80) 0.5-1.5 Shrub Thymol (60-70) 1.8-2.5 Shrub 8. - Ocimum viride - Chemical composition The oil extracted from the plant contain phenolic compounds such as carvacrol and eugenol; non phenolic compounds such as methyl cugcnol, methyl chavicol and caryophyllene etc. The leaves contain beta - carotene, sterols, fatty acids, proteins, carbohydrates, volatile oil, fixed oil, mineral matters and vitamin A, B and C. Major volatile oil (0.4-0.896) consists chiefly eugenol (-21%) and B - caryophyllene (37%). Eugenol content reaches maximum in spring and minimum in autumn (Baseer and jain 2016). A number of sesquiterpene and monoterpenes v Bommy acetate, -elemene, methyleugenol, neral B pinene, camphene etc. are found in oil. Ursolicacid, campestrol, cholesterol, stiprasterol, s -sitostorol and methyl esters are common fatty acids. The chemical compounds obtained from essential oil from various species have different types of constituents which are used in perfumes. Tulsi species, Ocimum basilicum contains 0.5–2.0 percent essential oil, Ocimum canum 2.0–4.0 percent, Ocimum americanum 3.6–6.5 percent. Ocimum Kilimandscharicum with 1.5-3.5 percent and Ocimum sanctum contains 0.5-10 percent (Kumar N 2018). The composition of oil recorded, on the basis of oil profile of different geographical location,is divided into four different types– (i) European type (sweet basil) (ii)reunion type (iii) methyl cinnamate type (iv) eugenol (wealth of india 1976, Sobit et al. 1982, Simoom et al. 1990, Maroti et al. 1996, Datta et al.2010, Gupta etal. 2018). Climate Basilis tropical to sub-tropical in nature which favors decently to high precipitation with high humid conditions.Strenuous day and high temperature condition upgrade the development of plant growth and oil production. Partial shade isnot favourableas it influences the oil content. The plant can tolerate drought season and can slightlyoffensivetowards virus conditions. The family Ocimum is an incredibly adaptable and isfound in tropical and warm temperature areas of the world. It grows well in moderate rainfall and humid conditions but simultaneously requires sunlight for growth and high oil content. Thefavorable temperature for plant growth varies between 24 ° C to 30 ° C.so, it can be cultivated only as a summer crop in hills (Srivastava et.al. 2009, Chang et al. 2005). Soil The plant is hardy in nature and it can be grown on a various type of soils like well drained sandy and sandy loam, saline alkaline and moderately acidic soils except the ones with highly saline andalkaline. However, sandy loam soil with good organic matter is considered ideal which have pH ranges between 5.0 to 8.5. The water logged conditions is not suitable for its survival. Varieties Ocimum basilicum CSIR-CIMAP has developed high yielding varieties of Ocimum basilicum namely CIM-Soumya, CIMSnigdha, CIM-Surbhi, CIM-Sarda, CIM-Jyoti, Vikarsudha, Kusmohak, MC-05. CIM-Soumya- This variety was developed through selection. It is short duration crop (3 months).The plants are dwarf and early flowering. Fresh herb yield is 290 q/ha and dry herb yield is 197 kg/ha with oil content 0.68% from fresh herb, 0.99% from dry herb. The methyl chavicol content remain 62.54% and linalool content 24.61%. CIM-Snigdha- This variety was developed through intensive breeding approaches. It is short duration, early maturing, high yielding and suitable for rain fed condition. It’s herb yield is approx. 280-290 q/ha, oil yield 190-200 kg/ha and methyl chavicol upto 85-89%. CIMA-Surbhi- This variety was developed through conventionalbreeding. It’s oil yield ranges 100-120 kg/ha, linalool 70-75% with 99% purity. CIM-Vikarsudha- This varity was eveloped through intra- specific hybridization between exotic basil from Australia (EC-331886) a landrace (badaun). It is a tall (75-90 cm) late maturity, herb yield 335 q/ha, oil yield 167 kg/ha, oil content 0.5%, methyl chavicol 78% and linalool upto 0.1%. Kusmohak- it is introduction from Argentina and developed through selection. It is a medium tall, high yielding, superior oil quality, sutaible for perfumery, cosmetic and related industry. Herb yield is upto 391 q/ha, oil yield 134 kg/ha, methyl chavicol 37% and linalool 45%. MC-05-The fresh herb yield of this variety is 36 tonnes/ha, oil yield 127.4 kg/ha, oil 0.35%, methyl chavicol 87%. RRL-07- This variety was developed from Regional research laboratory, Jammu. It high citral content (75-80%). Thefresh herb yield is 40 tonnes/ha, oil yield 200 kg/ha and oil content 0.50%. RRL-011- It is also developed from Regional Research Laboratory, Jammu. Fresh herb yield is 50 tonnes/ha, oil yield 220 kg/ha, oil content 0.50%, linalool 40% and methyl chavicol 35%. Ocimum sanctum-CSIR-CIMAP has developed followinghigh yielding varieties of Ocimum sanctum namely CIM-Ayu, CIM-Angana, CIM-Kanchan. CIM-Ayu-It is developed through mass selection. The plant of this variety is tall having green leaves, high eugenol content. The fresh herb yield is 200 q/ha, dry herb yield 15.85 q/ha, oil yield 110 kg/ha, oil content 0.72%and eugenol 83%. CIM-Angana- This variety is developed through half- sib selection. Plant of this variety is tall, with green leaves, suitable for tea. The fresh herb yield is 181q/ha, dry leaf yield 14.33 q/ha, oil yield 91.71kg/haand eugenol 40.42%. CIM-Kanchan-This is developed through selection. It is a medium tall havinglight green slightly undulated leaves with light purple stem. The herb yield is 19.7 tones/ha, oil yield 94 kg/ha and methyl eugenol 70%. Hybrid varietiesCIM- Shishir CIM-Suvash Ocimum africanum- CIM-Jyoti was developed through selection by CIMAP. It is a dwarf early maturing, white flower, herb yield 200 q/ha, oil yield 150 kg/ha and citral content 68-75%. Ocimum canumRRL-01- This variety was developed from Regional Research Laboratory, Jammu. Ocimum americanum- RRL-02 Ocimum biride- RRL-08 Ocimum gratissimum- RRL-08 Field preparation The field should be deep ploughingwith the help ofdisk plough in summer.Farm yard manure should be applied 12-15 ton/ha before or after first ploughingso that FYM canbe spared and mixed with the soil during second and third ploughing. Furthertwo ploughing should be done with the cultivatorfollowed by planking in order to break the clods aswell as to make the soil pulverize.Besides, weedstubbles and other grasses arealso removed manuallyto make the soil free from weeds. Now, the field becomereadyfor planting. Propagation Propagation of basil can be done by seed and/or vegetatively but commercial propagation is generally done by seed as this method is very cheap and easy. Vegetative method of propagation is not being used for commercial cultivation due to high labour cost. Sowing of basil can be done through direct sowing (broad casting)or in rows. However, in small farming it can be done through transplantation. 1. Direct sowing About 2-2.5 kg seeds are required for sowing of one hectare area. The seed is mixed with 8-10 timeswith fine dried soil or sand to ensure an even distribution in field. The spacing of plants can not be maintain through broadcasting so to overcome this problem the field is market into rows 50-60 cm apart. The seed with soil or sandis sown by seed drill or handin the rows but seed depth is kept not more than 2 cm. After sowing of seeds, the rows are covered with soil by foot or byusing light leveler. The irrigation is done after 24 hours of sowing (Bahl et al. 2018, Srivastava et.al. 2009). If the seeds are sown through broadcasting in the field without row than thinning is necessary for maintaining proper spacing. 2. Nursery preparation Basil is commonly raised through nursery and appropriate time for raising the nursery is last week of May to first week of June in north India and last week of march to first week of April in south India.The side of nursery isselected near to irrigation channels.About 500 m2 areaof nursery are required for the cultivation of one-hectareland. Before preparation of nursery the land is well pulverized and is free from weeds. The size of nursery bed is kept 2x4 m2 and the seedlings are transplanted when its height reaches upto 15-20 cm.Drainage channel are alsoprepared around the bed for preventing the nursery from water lodging. About 1ton FYM and 15:20:10 kg nitrogen, phosphorous, potash respectively is applied in the bed.500g seeds are required for raising nursery forone-hectare area. The seeds should be mixed with 810times fine dried soil or sand. The seedsare raised in 10-12 cm apart line and 1.5 - 2 cm depth in the soil. The appropriate moisture is kept in thebed.The nursery bed is mulched with hay and straw after raising nursery so the moisture canmaintainon hand and other hand sown seedscan be saved from the birds that is removedlater onafter germination ofseeds. The seedsare germinatedwithin8-12 days and the seedlings become ready for transplantationafter25-30 days (Srivastava etal. 2009). Transplanting The nursery isirrigated before transplantation and transplantationis usually done in the morning time. The seedlingsare planted in row at spacing 50*40 cm2 or 50*30 cm2depending upon soil fertility (Bahl et al. 2018). Intercropping Occimum is a small to medium bushy, annual and/or perennial herb that caneasily be grownunderneath of some biggest tree orchard viz. mango, guava, papaya etc. and also under themedium to tall annual and perennial crops viz. pigeon pea, maize, jowar, bajra. Manure and Fertilizer Generally, fertilizers are not required for plant growth but for good recovery of oil, fertilizer should be applied based on the soil fertility. Generally 50:60:40 kg nitrogen, phosphorus and potash are required per hectare. The 1/3 dose of nitrogen and full dose of phosphorus and potash are applied at planting time and last two doses are applied in two splitsduring the growing session (Srivastava et.al. 2009). Irrigation First irrigation is done just after transplantation and later 2-3 irrigationsare doneduring10-15 days interval. Irrigation depends on sowing time and availability of moisture content in the soil. Generally3-4 irrigations are essential but is not required during rainy session. The irrigation is stopped 10-15 days prior of harvesting. Intercultural operation Proper weeding is essential for successful growth of basil. Weed should be control before they srart competing for nutrient, moisture and light. First weeding is done one month after planting and second weeding is done 30-35 daysafter first weeding. Further weeding is not required because plants become busy thereby suppress the weed. Mulching can be done two months after planting toinhibit weed and maintain moisture. Chemical herbicide should not be used to control weed. Plant protection Insect-pest Generally, basil is a hardy crop and itvery less affected by insect, pest and diseases but some time the insect and diseases attack to the crop hencethe management is necessary to obtain maximum return. Leaf rollers- it affect thebasil crops which stick under the surface of the leavesand foldthe leavesbackwards lengthwise and alsotogether. Management- sparing of quinalphos 25 EC @ 300-400 ml per hectare, and spray of Malathion (0.2%) to control this insect. Lace wing- it is another pest which affect to the basil by ceasingthroughCochlochi labullita. The adult and nymphs feed on leaves and younger stems, sometimes gregariously and leave their excreta making it unsuitable for uses. Due to feeding, the leaves initially get curled and later on whole plant gets dried up. Management- Sprayingof Azadirachtin 10,000 ppm @ 5 ml/l remain effective to control this insect (Smitha et al. 2014 and Srivastava et.al. 2018). Aphids - Aphids are small, soft-bodied insects that are most commonly green and black in color, but may also be gray, brown, pink, red, yellow or lavender. They tend to feed on tender, young growth causing it to appear puckered or deformed. Though they are visible to the naked eye, they also leave behind an excretion known as honey dew, which is another method of identification. Management-it can be by botanical sprays such as insecticidal soap, pyrethrum, rotenone. Beneficial insects such as ladybugs will also help to eradicate the pest. Diseases Powdery mildew (oidium spp.), seedling blight (Rhizoctonia solani) and root-rot (Rhizoctonia bataticola) are major diseases of basil.Young plants remain more susceptible than old basil plants by downy mildew.Downy mildew- It is caused by Peronosporabelbahrii, the symptoms of downy mildew can be spotted on lower side of leaves and leaves become yellow. Management-Powdery mildew can be controlled by spraying wettable sulphur (4 g/litre of water). Root-Rot- it is ceased byRhizoctonia bataticola, the roots of basil plants get rot. Management- it can be managed by improved phyto-sanitary measures and by drenching the nursery beds with Bavistin 1% (Smitha et. al. 2014 and Srivastava et.al. 2018). Leaf Spot- bacterial leaf spots are encouraged by wet, humid conditions and disseminated through splashing water such as rainfall,black spots or lesions appears on the leaves. Management-effected plant should be remove from the field and it can be prevent diseaseresistant seed or plant material. Maintain good air circulation through consistent harvesting. Fusarium wilt-it is a soil based common diseases of basilcaused by fusarium oxysporum. The first symptom is yellowing shoot as well as yellowing leaves. In wet condition, the can show white or orange fungal growth on the stem, in the late stage of disease, the plant could develop twisted stem and the stem tissue become discolored. Management-infected plant should be remove and burn as soon as possible after identifying. Refrain from planting basil about 4-5 years in the infected field or soil. Disease fee seeds should be use for propagation. Crop rotation Crop rotation is important practice for maintainingthe soil fertility and high profit return. Tulsi crops is a high profitable crops compareto other some cereal crops, thus the planting of crops rotation of cereal crops with tulsi can proved high return. The following rotation are recommended. Basil-wheat-mint, Basil-pea-mint, Basil-potato-mint, Basil-potato, Basil-brassica-mint, Basil-camomilemint (Srivastava et.al. 2009, 2018) Harvesting The crop becomeready for first harvesting about 75-90 days after planting. The crop should be harvested when field is driedand sunny weather. The crop is cut 20-25 cm above from the ground level with the help of cutter. For distillation purposes, crop is harvested at 50 percent flowering. Distillation The distillation of basil is done by hydro- steam distillation using field distillation unit. Before distillation, the crop is dried in shade about 25-30 hours toremove the moisture. The dry herbage is filled in the tank of field distillation unit and heated. The process of distillation is completed about 3.5- 4 hours dependingupon the unit and heat. The oil is collected and stored in air tide aluminum pots. Yield An average herbage yield of basilis about 25-30 tons ofand about 120-150 kg of essential oil can be obtained per hectare. Storage The basil herbs are used for medicinal and aromatic purposes. For medicinal purposes especially in ayurvedic system fresh and dry leaves are mostly used to maintain its valuable compound in the herbage.The storage of basil is important process. The high percentage of water availability in the plant (about 92%) makes basil quite perishable thus it is stored in a cool place or in the refrigerator for some time. Dehydration is the most common and cheapest way of preserving basil leaves ensuring microbial safety and extending their shelf-life. After harvesting the fresh herb is storedvery carefully in shade and cool place because over drying of leaves reduced the recovery of oil content. After distillation of basil, essential oil is storedin air tied aluminium container for a long period. The container size should be such that can fullyfilledwith the oil without air and the container is kept in dry and clean place. Cost Benefit ratio S.N. Cultural practices Cost of (Rs./ha/year) 1. Land preparation 2800 2. Seed (1 kg) 2500 3. Transplanting 3000 4. Irrigation 900 5. Fertilizer 2850 6. Plant Protection 700 7. Weeding 2400 8. Harvesting 3600 9. Transport 1000 10. Distillation 3000 11. Interest of variable cost 796 Total 23546 Total yield per ha- 120 kg, Price of oil per kg 1000 (Price of oil can be fluctuating) cultivation Gross return (Rs.) - 1,20,000 Net return (Rs.) - 96,454 Cultivation practices in brief S. N. 1. Cultivation practices Field Preparation 2. Seed 3. 4. 5. Nursery preparation Transplantation Irrigation 6. Fertilization 7. 8. Weeding Plant Protection Time May- June Description First deep ploughing withdisk harrow in summer after then 2-3 plowing by cultivator After preparation of field 500g/ha for nursery and 1.5-2 kg/ha for direct sowing Last week of May- June 500g seed and 500 m2 per ha area are required June - July After 25-30 day of nursery sowing Just after plantation Three to four irrigation are required in 25-30 day interval During field preparation and 50:60:40 NPK/ha after irrigation 20-25 day after plantation Two to three weeding are required Pest- Fusarium wilt, Damping off, lace wing. Diseases- powdery mildew, seedling blight, root-rot. 9. Harvesting 10. Distillation 11. Yield 12. Storage 75-90 days after plantation Cut the plant by cutter 10-15 cm above from ground level 20-25 hrs. after harvesting Hydro stem distillation about 3.5-5 hrs take in distillation. After harvesting and 25-30ton herbage and 120-150 kg/ha oil distillation Herbagebefore distillation Herbage store in shade, dry and cool and essential oil after place and essential oil store in air tied distillation aluminum container References Bilal A, Jahan N, Ahmed A, Bilal SN, Habib S and Hajra S (2012) Phytochemical and pharmacological studies on Ocimum basilicum L. a review. International Journal of Current Research and Review 4(23):73–83. Baseer M, JainK (2016) Review and botany, phytochemistry, pharmacology, contemporary application and toxicology of Ocimum sanctum, international journal pharm life science 7(2):4918-4929. Baiswas NP, Biswas AK (2005) evaluation of some leaf dusts as grain protectant against rice weevil sitophilusoryzae (linn), environ ecol 23:485-288. Balyan SS, Pushpangadam P (1988) A study on the taxonomy status and geographic distribution of genus Ocimum, Pafai J 10(20):13-19. Baczek K, Kosakowska O, Gniewosz M, Gientka I, Weglarz Z (2019) Sweet Basil (Ocimum basilicum L.) Productivity and Raw Material Quality from Organic Cultivation. Agronomy 9, pp 279. [CrossRef] Bahl JR, Singh AK, Lal RK, Gupta AK, (2018)High yielding improved varieties of medicinal and aromatic crops for enhanced income. In: Singh, B, Peter KV (eds) new age herbals. Springer Nature Singapore Pte Ltd, pp 257-259. Change X, Alderson P G, Wright CJ (2005) Effect of temperature integration on the growth and volatile oil content of basil (Ocimum basilicum L.), Journal of Horticultural Science & Biotechnology, 80 (5):593–598. Chopra RN, Nayer SI, Chopra IC (1996) Glossary of Indian medicinal plants. CSIR, new delhi. Darrah HH (1974) Investigations of the cultivars of basils (Ocimum). Econ. Bot.28:63–67. Darrah HH(1980)The Cultivated Basils. Buckeye Printing Co., MO. pp82. Datta AK, Mukherjee M, Bhattacharya A, Saha A, Mandal A, Das A (2010) the basils- an pverview. JTrop Med Plants 11(2):231-241. Gupta SM (1971) Plant myths and traditional in india. E. J. Brill, Leiden, Vangsgards Antikvariat Aap (Copenhagen, Denmark), pp 66-72. Gille E, Danila D, StanescuU, Hancianu M (2007) The phytochemical evaluation of some extract of ocimum sp. Planta med 73 (09):374. Govindrarajan R, Vijayakumar M, Pushpangadan p (2005) Antioxidant approach to disease management and the role of ‘Rasayana’ herbs of Ayurveda. J Ethnopharmacol 99(2):165-178. Ghosh GR (1995) Tulasi (genus Ocimum). New Approaches to Medicine and Health (NAMAH) 3, pp 23–29. Grieve M(1999)A Modern Herbal. Tiger Books International, London, UK.pp. 85-87. Gupta S, Srivastava A, Shasany AK, Gupta AK (2018) Genetics, Cytogenetic and genetic diversity in the genus ocimum. In: Shasany, AK and Kole, C. (eds.) The Ocimum genome, springer, pp 73-87. Grayer RG, Kite GC, Goldstone FJ, Bryan SE, Paton A, Putievsky E (1996) Infraspecific taxonomy and essential oil chemotypes in basil. Ocimum. Basilicum. Phytochem. 43:1033–1039. [CrossRef] Heath HB (1981) sources book of flavors. AI, Westport, CT. Hiltunen R, Yvonne H (eds.) 1999. Basil: The genus Ocimum. (Medicinal and Aromatic Plants— Industrial Profiles, 10). Amsterdam: Harwood Academic. [Republished 2005 Taylor & Francis eLibrary.] Hakkim FL, Arivazhagan G, Boopathy R (2008) Antioxidant property of selected Ocimum species and their secondrymrtabolitr content. J med plant res 2(9):250-257. Hereman S (1868) Paxton’s Botanical Dictionary, Bradbury Evans and Co., London.Google Scholar Joshi RK (2017) Phytoconstitutents, traditional, medicinal and bioactive uses of basil (ocimum sanctum L.): a review. J Pharmacognology phytotochen 6(2):261-264. Kokwaro JO (1976) medical plants of east Africa. East African literature bureau, general printers limited, Nairobi 384. Kumar A, Shukla AK, Shasany AK, Sundaresan V (2018) Systematic position, phylogeny, and Taxonomic revision of india, Springer, pp 61-72. Kumar N (2018) Introduction to spices, plantation crops, medicinal and aromatic plants, medtech (3):293-297. Makri O, Kintzios S(2007) Ocimum sp. (basil): Botany, cultivation, pharmaceutical properties, and biotechnology. J. Herbs. Spices Med. Plants, 13, 123–150. [CrossRef] Morales MR, JE Simon (1996) New basil selections with compactinflorescencefor the ornamental market, In: J. Janick, ed. Progress In New Crops. ASHSPress, Alexandria, VA. pp. 543-546. Marotti M, Piccaglia R, Giovanelli E (1996) Differences in essential oil composition of basil (ocimum basilicum L.) Italian cultivars related to morphological characteristics. J Agric Food Chem 44:39263929. Monier W, Monier (1899) A Sanskrit-English dictionary etymologically and philologically arranged with special reference to cognate Indo-European languages. With the collaboration of Professor E. Leumann, Professor C. Cappeller, and other scholars. Oxford: Clarendon. [Reprinted 1956.] Mukherjee M, Dutta AK (2007) Basil- a review, plant arch 7(2):473-483. Proenca dC, Adzet T (1999) Composition of the essential oils of O. canum,O. gratissimumand O. minimum. Planta Med. 65:187-189. Miele M, Dondero R, Ciarallo G, Mazzei M (2001) Methyleugenol in Ocimum basilicum L. Cv. ‘Genovese Gigante’. Journal of Agricultural and Food Chemistry 49, 517–521. Nacar S, Tansi S(2000) Chemical components of different basil (Ocimum basilicum L.) cultivars grown in Mediterranean regions in Turkey. Israel J. Plant Sci. 48, 109–112. [CrossRef] Paton AJ, Harley RM, Harley, MM (1999) Ocimum: an overview of classification and relationships. In: Hiltunen R, Holm Y (eds) Ocimum. Medicinal and aromatic plants- industrial profile. Harwood Academic, Amsterdam, pp1-38. Puri HS (2002) Rasayana: Ayurvedic herbs for longevity and rejuvenation. CPC Press, Boca Rton, pp 272-280. Prakash p, Gupta N (2005) therapeutic uses of Ocimum sanctum linn (tulsi) with a note on eugenol and its pharmacological actions: a short review. Indian j physiolpharmacol, 49(2):659-666. Pushpangadan P, BraduBL (1995) Basil, In: K.L. Chadha and R. Gupta,eds. Advances in Horticulture, Medicinal and Aromatic Plants, MalhotraPublishingHouse, New Delhi, India. (11): 627-657. Prasad G, kumar AM, Singh AK, Battacharya AK, Singh K, Sharma VD (1986) Antimicrobial activity of essential oil of some Ocimum species and clove oil. Fitoterapia 57:429-432. Srivastava RK., Shukla SV, Dagar SS. (2009) cultivation and uses of aromatic plants. IDBC publishers, lucknow, india. Pushpangadan P, Bradu BL (1995) Medicinal and aromatic plants. In: Chadha KL, Gupta R (eds) Advance in horticulture. Mathura publication house, new Delhi, pp 627-657. Suddee S, Paton AJ, Parnel JAN (2005) Taxonomic revision of tribe ocimeae Dumort . (Lamiaceae) in continental south east asia III. Ociminae. Kew bull 60:3-75. Sobti, SN., Pushpangadam,P (1977) Cytotaxonomical studies in the genus I. In: Bir SS (ed) Taxonomy, cytogenetics, cytotaxonomy of plants. Kalyani publisher, new delhi india pp 373-377. Simon, JE., Quinn, J., Murray, RG (1990) Basil: a source of essential oil. In: Janick J, Simon JE., (eds) Advances in new crops. Timber, Portland, pp 484-989. Shah S, Rastogi S and Shasany AK (2018) Genomic resources of ocimum, The ocimum genome (springer) pp 99-110. Simon JE, Quinn J, Murray RG(1990) Basil: A source of essential oils. In Advances in New Crops; Janick, J., Simon, J.E., Eds.; Timber Press: Portland, OR, USA, pp. 484–489. Smitha GR, Thania S, Manivel VP (2014) Technical bulletin of basil, directorate of medicinal and aromatic plant research. Anand Gujrat, India. Wealth of India (1976) A dictionary of india raw material and industrial productios, publication and information directorate, CSIR, new delhi (3):77-89. Ocimum basilicum HybridTusli Kapoor Tulsi Ocimum sanctum CIM Krishna Intercropping with Maize Lemon Tulsi Field Preparation