ISOLASI DAN IDENTIFIKASI MOLEKULER BAKTERI AMILOLITIK
PADA PRODUK FERMENTASI GADUNG (Dioscorea hispida Dennst)
BERDASARKAN SEKUEN GEN 16S rRNA
Rizka Khoirunnisa1, Norma Stalis Ethica2, Ayu Rahmawati Sulistyaningtyas3
1.
2.
3.
Program Studi D IV Analis Kesehatan Fakultas Ilmu Keperawatan dan Kesehatan
Universitas Muhammadiyah Semarang
Laboratorium Biologi Molekuler Fakultas Ilmu Keperawatan dan Kesehatan
Universitas Muhammadiyah Semarang
Laboratorium Mikrobiologi Fakultas Ilmu Keperawatan dan Kesehatan
Universitas Muhammadiyah Semarang
ABSTRAK
Enzim amilase merupakan enzim yang dapat ditemukan pada mikroorganisme, hewan
dan tanaman. Salah satu cara untuk mendapatkan enzim amilase dari bakteri yaitu
dengan melakukan isolasi bakteri dari produk hasil fermentasi amilum. Salah satu
sumber makanan yang menghasilkan karbohidrat adalah umbi gadung. Gadung
mengandung karbohidrat dengan kadar sebesar 18%, lemak 0.16%, protein 1.18%,
serat kasar 0.93%, dan kadar abu 0.69%. Berdasarkan hasil isolasi bakteri yang berasal
dari umbi gadung paska fermentasi 72 jam didapatkan 4 isolat bakteri murni yaitu :
FDH-1, FDH-2, FDH-3, dan FDH-4. Setelah dilakukan isolasi dan identifikasi koloni
serta dilakukan uji aktivitas hidrolitik menggunakan media Starch agar isolat FDH-1
dipilih untuk isolasi dan identifikasi molekuler untuk dilanjutkan isolasi DNA, PCR,
elektroforesis yang kemudian diproses BLAST dan pensejajaran hubungan
kekerabatan. Isolat FDH-1 memiliki cabang yang sama dengan Pseudomonas stutzeri
strain Os. menunjukkan isolat FDH-1 memiliki hubungan kekerabatan yang sama
Pseudomonas stutzeri strain Os. Berdasarkan hasil yang diperoleh melalui produk
fermentasi umbi gadung dengan demikian strain bakteri Pseudomonas stutzeri diberi
nama isolat bakteri Pseudomonas stutzeri strain FDH-1.
Kata Kunci : Amilase, Gadung, Pseudomonas stutzeri strain Os.
ISOLATION AND IDENTIFICATION OF AMYLOLITIC BACTERIA
MOLECULARS ON FERMENTATION PRODUCTS OF GADUNG (Dioscorea
hispida Dennst) BASED ON GENE SEQUENCE 16S rRNA
Rizka Khoirunnisa1, Norma Stalis Ethica2, Ayu Rahmawati Sulistyaningtyas3
1.
2.
3.
Four years Diploma of Medical Laboratory Technology Study Program, Nursing
and Health Faculty, Muhammadiyah University of Semarang
Biology Molecular Laboratory, Nursing and Health Faculty, Muhammadiyah
University of Semarang
Microbiology Laboratory, Nursing and Health Faculty, Muhammadiyah
University of Semarang
ABSTRACT
Amylase is an enzyme that can be found in microorganisms, animals and plants. One
way to get the amylase enzyme from bacteria is by isolating bacteria from the
fermented starch products. One source of food that produces carbohydrates is the
gadung tuber. Gadung contains carbohydrates with content of 18%, 0.16% fat, 1.18
% protein, 0.93% crude fiber, and 0.69% ash content. Based on the results of bacterial
isolation from gadung tubers after 72 hours of fermentation, 4 pure bacterial isolates
were obtained, namely: FDH-1, FDH-2, FDH-3, and FDH-4. After isolation and colony
identification were carried out and hydrolytic activity tests were carried out usingmedia
Starchso that the FDH-1 isolate was selected for isolation and molecular identification
to be continued with DNA isolation, PCR, electrophoresis which was then processed
by BLAST and alignment of kinship relationships. FDH-1 isolate has the same branch
as Pseudomonas stutzeri strain Os. showed that FDH-1 isolates had the same kinship
relationship as Pseudomonas stutzeri strain Os. Based on result obtained through a
product fermentation of gadung, a strain of bacteria Pseudomonas stutzeri is named a
Pseudomonas stutzeri strain FDH-1.
Keyword : Amylase, Gadung, Pseudomonas stutzeri strain Os.