Introduction to Cell Cell All organisms are made of cells The cell is the simplest collection of matter that can live Basic structural and functional unit of life To study cells, biologists use microscopes and the tools of biochemistry Cytology It is the study of the structure and function of cells. The cell is the basic structural and functional unit of life so therefore cytology is the fundamental most important topic in all of biology. Importance An understanding of cell biology is important in many areas of study, for the cell is the building block of all living forms. This course complements studies in any area of applied biology including human health and fitness, horticulture, agriculture and wildlife management. How do we observe cells? Light microscopes (LM.s) Pass visible light through a specimen Magnify cellular structures with lenses Electron microscopes (EM.s) Focus a beam of electrons through a specimen (TEM) or onto its surface (SEM) People who were important in early cell discovery Robert Hooke (1665) Observed dead cork - called them “cells“ Compound Microscope Anton Von Leeuwenhoek (1674) - living cells in pond water; one celled organisms -- animalcules Robert Brown (1831) identifies the nucleus of a cell Mattias Scleiden (1838) stated that plants are made up of cells Theodor Schwann (1839) stated that animals are made up of cells Rudolph Virchow (1858) 1. 2. Studied the pathology of cells. (ability to cause disease) All cells arise from preexisting cells. New cells can only arise from other living cells by the process of cell division or reproduction Cell Theory 1. All living things are made of cells. (basic structure). 2. All cells carry out life functions. (basic function) 3. All cells come from pre-existing cells. All living things are composed of cells. Prokaryote – a cell without membrane bounded organelles Eukaryote – cell with defined membrane bounded organelles Unicellular - composed of one cell Multicellular - composed of more than one cell. Levels of organization Cell- basic unit of life. Tissue- group of cells performing the same function. Lung tissue Organs- group of tissues performing the same function. Lung, heart Organ system- group of organs that perform the same function.ex. Respiratory system Organism- group of organ systems working together. PROCESSES OF CELLS All life processes involve energy changes. 1. Nutrition - food is needed for energy and building materials. 2. Digestion - breaking down reactions of food into usable parts. 3. Absorption - water, food, ions and other materials 4. Biosynthesis - cells organize many organic substances for cell activity. 5. Respiration - cell energy is released when certain organic molecules are split - energy is used for cell activity. 6. Excretion - waste materials passed from cell to environment 7. Secretion - synthesized molecules which are passed out of a cell and which affect the activities of other cells (vitamins, hormones) 8. Reproduction - cells divide; unicellular - more organisms, multicellular - more cells. 9. Movement - motion of all types; cellular contractions, flowing substances within the cell 10. Egestion - elimination of insoluble compounds and nondigestible particles Prokaryote Eukaryote Eukaryotic cells – advanced cells Have nucleus Plasma membrane Cytoplasm - everything between plasma membrane and nucleus Organelles Fluid Cytoskeleton – threads of microtubules and microfilaments in cytoplasm Differences between plant and animal cell Plant cell Cell wall No centrioles 1 Large vacuole (Tonoplast) Chloroplast (chlorophyll) Animal cell No cell wall Centrioles present Many small vacuoles No chloroplasts Plant parts Cell wall- rigid, made of cellulose and gives support and structure. Chloroplasts- contain chlorophyll, photosynthetic organ Tonoplast- large vacuole that gives plant support. Cell Size Largest cell in the world The egg from an ostrich 15cm Other parts of a Cell Ribosome- synthesis proteins Nucleus- controls cell activity Cell membrane- regulates what enters and leaves the cell Mitochondria- carries out cellular respiration to release energy. Endoplasmic reticulum- system of channels that transport materials. Smooth ER- without ribosome Rough ER- with embedded ribosome. Lysosomes- digestive enzymes to break down substances. Nucleolus – synthesize ribosome. Cytoplasm- liquid portion of the cell Vacuoles – storage bins of the cell. Structure of cell wall Cell wall is present only in plant cells. Cell wall is non-protoplasmic. It is considered as metabolic byproduct of the protoplast. Cell wall provides support and protection to cell. It also plays an important role in absorption, translocation and secretion. Typical component of plant cell that fixes the size and shape of the mature plant cell. The kind of cell wall present determines the texture of a tissue. Functions Mechanical functions –Like a skeleton around each cell –Determine shape and size of cell –Determines the limits of expansion and water uptake •Metabolic Activity –Living part of cell –Receives signals such as hormones. –Wall signals transmitted through plasmalemma into cytoplasm, -biochemical response •Absorption or secretion–root hairs, rhizoids. Cell wall is divided into different layers. Cell walls vary greatly in composition and morphology. It is composed of different layers: The cell walls vary much in thickness in relation to age and type of cells. Young cells have thinner cell walls than the fully developed ones. •The cell wall is complex in its structure and usually consists of three layers: •Primary cell wall( usually consists of one layer •Intercellular substance(middle lamella) cements together primary wall of two adjacent cells. Secondary cell wall((made up of one-many layers , frequently three) 1. Middle lamella It is the outer most layer of cell wall. The cell wall of the adjacent cells is joined by middle lamella. Middle lamella is a thin layer. Cellulose is absent in it. It is mainly composed of pectic compounds. Middle lamella acts as an intercellular substance. It holds the cell together. 2. Primary wall Primary wall is elastic and transparent. It is the first wall formed in the developing cell. It remains primary in many types of mature cells. It is present only in the living cells like meristems and other living tissues. But it is absent in non-living cells like tracheids, sclerenchyma etc. Primary wall undergoes extensive growth with the increase in size of cell. It is chiefly formed of cellulose and hemicelluloses and pectic material. The increase of thickness of primary layer occurs due to deposition of successive layers of cellulose towards the inner side of the cell. Lignin is deposited between the primary wall and middle lamella in mature tissues. Therefore, primary wall cannot be differentiated from the middle lamella. The primary wall in many cells has lamellae like structures called microfibrils. These microtibrils are arranged in criss cross manner. (3) Secondary wall The increase of surface area of primary wall stops with the growth of cell, now secondary wall is formed inner to the primary wall. secondary wall has no surface growth. It has compounds like pectin, silica, gum etc. Unequal lignification produces following thickenings in the cell wall: Annular: These are ring like thickening. In this case, ring of lignin are deposited one above the other. Scalariform: These are ladder like lignin thickenings Spiral: In this case, thickenings are arranged in spiral bands. Reticulate: In this case, lignin thickenings form networks. Pitted: The entire cell wall is thickened. It has only few tin areas called pits. Plasmodesmata The plasmodesmata are cytoplasmic strands that extend through the pores in adjacent cell walls. The protoplasts of the adjoining cells are not completely separated by the cell walls. The protoplasts of adjoining cells are connected by fine strands. These strands pass through pores and form plasmodesmata. Plasmodesmata are concerned with the transport of material & stimuli from cell to cell. Plasma Membrane OUTSIDE OF CELL Sugar Chain Lipid Bilayer Protein Marker Cholesterol INSIDE OF CELL Proteins Embedded Protein Cell Membrane Side Profile Different membranes All have similar functions & structures Plasma membrane separates inside of cell from outside of cell Other membrane define organelles to form compartments of eukaryotic cells Forms a selectively permeable layer Lets some things in or out but not all Like a window screen Nucleus - Structures Envelope Chromatin Double membrane Pores to get messages in and out DNA threads Protein balls called histones - wrapping Nucleolus - site of ribosome production Ribosomes Consists of 2 parts, which are made in nucleus Make protein in the Cytoplasm Produce proteins from ‘recipes’ in the nucleus copied into mRNA Some (proteins) will remain in cytoplasm Some will be exported out of cell Some will attach to membranes in cell Ribosomes are found on the endoplasmic reticulum and in the cytoplasm Endoplasmic Reticulum Rough ER Smooth ER Rough Endoplasmic Reticulum Rough ER - attached to nucleus Ribosomes stud surface Produces Membrane proteins - stay in cell Secretory proteins - exported from cell RER makin’ Proteins! Smooth Endoplasmic Reticulum No ribosomes, so not protein factories Makes steroids, lipids Liver cells have lots of smooth ER Detoxify chemicals Supply and demand Increases tolerance to drug So higher doses needed for same effect Golgi apparatus – the Post Office of the Cell Golgi apparatus – what it does Refines, stores and marks molecules for shipment Looks like stack of hollow pancakes Products of ER arrive & leave via transport vesicles Moving from one sac to the next Molecules get modified Labeled and / or stored Called the cell “Post office” because it marks and directs products in the cell Lysosome Lysosome Greek for breakdown body Sac of strong digestive enzymes Recylcer Compartmentalized for safety Can release to breakdown entire cell – “suicide sack” Functions Digest food vacuoles Digest invading bacteria Digest old organelles Lysosome Lysosomal diseases Genetic disorders ‘Recipe’ is messed up so protein doesn’t work If recipe for lysosome enzyme What should get broken down doesn’t Ex. Tay Sachs Lipids aren’t broken down Build up occurs Eventually causes death Usually in before age 5 Mitochondria Mitochondria Site of cellular respiration Conversion of food into energy (ATP) Double membrane ATP is what cells use to make things happen (drive chemical reactions) Big bag stuffed in smaller bag Folds of inner bag called cristae Space inside inner bag called matrix Also once free living bacteria Efficiency - gasoline engines converts 25% of energy mitochondria converts 54% of energy contains some of its own DNA (amount varies within organisms) believed to evolved from a primitive cell engulfing it and creating a symbiotic relationship DNA in mitochondria obtained only from mother of organism. Plant Organelles Chloroplasts - in plants Chloroplast 1. chlorophyll is green chemical that releases electrons, working like a solar panel in sunlight 2. forms glucose 3. photosynthesis 6CO2 + 6H2 O ---> C6H12 O6 + 6O2 Chloroplasts Structure of Chloroplast Double membrane Grana Stack of thylakoids Stroma Hollow disk Where sunlight energy is captured and converted to chemical energy Thick fluid filling chloroplast Contains some DNA Once free-living bacteria Cytoskeleton Role of the Cytoskeleton Network of fibers Give shape to cells Allow movement of cell Move organelles around Made of microtubules and microfilaments Centrioles Cell reproduction; goes to poles of cell during cell division and helps cell divide Centrioles at Work in Cell Division Cell Specialization Different kinds of cells suited for a different activity. Division of Labor Different cells divide their labor each has a specific function and supports each other. Levels of structure 1. 2. 3. 4. 5. Cell Tissue Organ Organ system Organism Tissue Level A group of cells that are alike in structure and activity in an organism muscles - Skeletal muscle cells – motion - Cardiac muscle cells – heartbeat - Bones - support - Nerve cells - coordination, perception and automatic body functions Organ Level several tissues working as a unit Animals heart brain stomach Plants roots stem leaf Organ System many organs involved in carrying out a function digestive nervous skeletal excretory respiratory endocrine – (hormones) circulatory muscular reproductive Organism complete living thing cell tissue organ organ system organism