Virtual Lab: DNA Extraction from Cheek cells
Name: Tate Sorenson
Period: 5 Date: 9/16/20
Source: Genetic Science Learning Center. "DNA Extraction Virtual Lab." Learn.Genetics 14 July 2009
http://learn.genetics.utah.edu/content/labs/extraction/
Objective: In this virtual lab, you will identify the resources and process of DNA Extraction.
Introduction:
1. Name two (2) reasons scientists isolate DNA.
 Genetic Testing
 Body Identification
Step One: Collect Cheek Cells
2. What is contained inside the nucleus of every cheek cell? Two meters of DNA
Step Two: Burst Cells Open to Release DNA
3. “Lysis” is a Greek word that means what? To separate
4. The Lysis solution you added to your Eppendorf tube and placed in the warm water bath contained detergent
and enzymes.
a) What was the purpose of the detergent? Disrupt the cell membrane and nuclear envelope, causing the
cells to burst open and release the DNA.
b) What was the purpose of the proteinase K? Cuts apart the histones to free the DNA.
Step Three: Separate DNA from Proteins and Debris
5. What is the function of the salt solution? Causes proteins and other cellular debris to clump together.
6. What is the function of the centrifuge step? The protein sinks to bottom of the tube, while separating the
DNA from the clumps.
Step Four: Isolate Concentrated DNA
7. Isopropyl alcohol is used to precipitate out the DNA because it is not soluble.
a) What does this mean? _______________________________________________________________
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8. Why must the new tube be centrifuged? ________________________________________________________
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Analysis and Conclusions
9. Why do you think it is important to have a “balanced” centrifuge? ___________________________________
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10. Relate how shampoo performs the same function on your hair as it does on cell membranes in this lab.
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