INTERNSHIP REPORT
CREATED BY:
• AZIZ NUR WIBOWO
• ILHAM PURNAMA
• M. FAJAR MAULANA
• NIKHEN SYAWINDARI
XII CHEMISTRY 2
COMPANY PROFILE
COMPANY NAME: PT. TIRTA INVESTAMA SENTUL
ADDRESS
: JL. OLYMPIC RAYA NO. KAV. A7, SENTUL, BABAKAN
MADANG, KABUPATEN BOGOR, JAWA BARAT 16810
BUSINESS FIELD : PACKED MINERAL WATER MANUFACTURE
1973, TIRTO UTOMO
ESTABLISHED PT.
GOLDEN MISSISSIPPI
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PT. AQUA GOLDEN MISSISSIPPI BEKASI
PT. AQUA GOLDEN MISSISSIPPI CICADAS
PT. AQUA GOLDEN MISSISSIPPI MEKARSARI
PT. TIRTA INVESTAMA MAMBAL, BALI
PT. TIRTA SIBAYAKINDO BERASTAGI
PT. TIRTA INVESTAMA PANDAAN
PT. TIRTA INVESTAMA MANADO
PT. TIRTA INVESTAMA BABAKANPARI
PT. TIRTA INVESTAMA SUBANG
PT. TIRTA INVESTAMA WONOSOBO
PT. TIRTA INVESTAMA LAMPUNG
PT. TIRTA INVESTAMA KLATEN
PT. TIRTA INVESTAMA KETON CANDI
PT. TIRTA INVESTAMA CIHERANG
PT. TIRTA INVESTAMA CIANJUR
IBLC Sdn BHd BRUNEI DARUSSALAM
PT. TIRTA INVESTAMA GATSU, BALI
PT. TIRTA INVESTAMA SOLOK
PT. TIRTA INVESTAMA LANGKAT
PT. TIRTA INVESTAMA SENTUL
1989, THE COMPANY
NAME CHANGE TO PT.
AQUA GOLDEN
MISSISSIPPI
AQUA GROUP
1. PT. TIRTA INVESTAMA
2. PT. AQUA GOLDEN MISSISSIPPI
3. PT. TIRTA SIBAYAKINDO
4 SEPTEMBER 1998, MAKE AN
ALLIANCE AGREEMENT WITH
DANONE ASIA HOLDING Pte. Ltd AS
AQUA GROUP
PT. TIRTA INVESTAMA
SENTUL
SOURCE HOUSE
WATER TREATMENT
WASHER
1. STORAGE GIANT TANK
2. UV OUTLET
3. CARTRIDGE FILTER 5
MIKRON
4. CARTRIDGE FILTER 1
MIKRON
5. MIXING KATADYN
6. FINISH TANK
7. FINISH PRODUCT
1.
2.
3.
4.
5.
FILLING
AQUA 5 GALLON PRODUCT
PREWASH
DETERGENT (MIPCIP)
RINSE
SANITIZER (OXONIA)
FINAL RINSE
ANALYZING ACTIVITIES
PHYSICS-CHEMICAL
ANALYZING
1.
2.
3.
4.
5.
6.
Ph CONTENT
CONDUCTIVITY
TURBIDITY
TEMPERATURE
ODOR & TASTE
SEDIMENTATION
TEST
7. SILVER CONTENT
8. NITRITE
CONTENT
9. TOTAL DISSOLVED
SOLUTION
10. ALKALINITY
1. DETERGENT
CONCENTRATION
2. SANITIZER
CONCENTRATION
3. HARDNESS OF
SOFTWATER
MICROBIOLOGY
ANALYZING
1. HETEROTHROPIC
PLATE COUNT (HPC)
2. TOTAL
YEAST
&
MOLDS
3. COLIFORM
4. PSEUDOMONAS
AERUGINOSA
OBSERVATION PROCEDURES
to analyze NITRITE CONTENT
TOOLS
MATERIALS
1. ERLENMEYER 250 ML
2. VOLUMETRIC
PIPETTE 1 ML
3. FILLER PIPETTE
4. SPECTROFOTOMETER
5. CUVETTE
1. AQUADEST
2. NAFTIL (N-1)
ETIENDIAMIN
DIHIDROKLORIDA
SOLUTION
3. SULFANILIC ACID
SOLUTION
WORK PROCEDURE
1. Prepare tools and ingredients
2. Pour 50 ml of the sample into a 250 ml erlenmeyer then add 1
ml of sulfanylic acid, homogenize then wait for 2-8 minutes
3. Add 1 ml of Naphthyl (1-N) Etiendiamin dihydrochloride,
homogenize it then wait for 10 minutes
4. Fill the cuvette with aquades and put it in the
spectrophotometer, press zero
5. Discard the contents of the cuvette then rinse with the sample
solution
6. Refill the cuvette with sample solution then put it into the
spectrophotometer, press read
7. Record the results
OBSERVATION PROCEDURES
to analyze ALKALINITY CONTENT
TOOLS
1. ERLENMEYER 250 ML
2. DROP PIPETTE
3. MEASURING CUP 100
ML
4. BURETTE
MATERIALS
1. MINERAL WATER
SAMPLE
2. Na-EDTA
3. SULFANILIC ACID
4. BCG INDICATOR
5. SULFURIC ACID
WORK PROCEDURE
1. Use lab coats, masks, latex gloves, safety glasses and
safety shoes before doing the analysis
2. 100 ml of sample is put into Erlenmeyer
3. Plus 2 drops of Bromocresol Green indicator
4. Titrated with Sulfuric Acid 0.02 N until the blue color
changes to light green
OBSERVATION PROCEDURES
to analyze HARDNESS
TOOLS
1. ERLENMEYER 250 ML
2. DROP PIPETTE
3. MEASURING CUP 100
ML
4. BURETTE
5. SPATULA
WORK PROCEDURE
MATERIALS
1. BUFFER
HARDNESS
2. EBT
3. EDTA
1.
2.
3.
4.
Add 100 ml sample
Add 0.5 ml hardness buffer
Add EBT 1 small spoon, until it turns purple
Titrate with EDTA until it turns blue
OBSERVATION PROCEDURES
to analyze COLIFORM
TOOLS
1. Holder & vacuum
2. Sterile petridisk
3. Membrane filter 0.45
micron
4. Camping gas
5. Cotton
6. Sterile tweezers
7. Laminar air flow
8. Incubator
MATERIALS
1. 70% Alcohol
2. CCA Media
3. Mineral water
sample
WORK PROCEDURE
1. Rinse the filter holder using cotton that has been dipped in 70%
alcohol
2. 0.45 micron filter membrane mounted on the filter holder
3. The sample was homogeneous by means of a bottle in the round 3
times
4. Opened the sample bottle cap, skip the mouth of the bottle on a fire
(fixation)
5. Pour 250 ml into the filter cup holder, wait until the water is always
filtered
6. The membrane filter is taken from the holder using a sterile tweezers,
put it in a petridisk that already contains the media, try not to touch
the middle of the membrane, afraid of being contaminated.
7. The petridisk was incubated in a good position in an incubator at 36 ° C
for approximately 1 ° C for 1 day (18 hours)
8. Read with a microscope if the colony is dark blue or purple, positive E.
Coli. And if the colony is red, positive is coliform