LAB #3 MICROSCOPE WORKSHEET
Name _____________________________
Lab__________________
Exercise 1A – Parts of the Compound Microscope (10)
Write the correct label for each part of the microscope shown below:
Exercise 1A – Functions of the Compound Microscope Parts (10)
Match each part of the compound microscope on the left with its function on the right:
____ stage clip
____ coarse focus knob
A. eyepiece, what you look in to see the image
B. adjusts position of slide left/right, front/back
____ condenser lens
C. used to bring the image into sharp focus
____ iris diaphragm
D. secures slide in place before viewing
____ fine focus knob
E. focuses light from the lamp on the slide
____ high power objective lens
F. used only with the low power objective
____ lamp
G. adjusts the amount of light passing through slide
____ low power objective lens
H. used only when you first examine a slide
____ mechanical stage knobs
I. light source used to illuminate specimen
____ ocular lens
J. used to produce the most magnified image
Exercise 1B & 2A – Total Magnification and Field Diameter (FOV) (20)
Fill in the chart below. For your actual microscope, you will find the magnifications of the ocular and
objective lenses printed on the side of each lens. Measure or calculate the field diameter for each total
magnification on your microscope in millimeters (mm) and then convert to μm. Remember 1000µm =
1mm
Table 3-8: YOUR MICROSCOPE MEASUREMENTS
Ocular Lens
Magnification
Objective
Lens Name
Objective Lens
Magnification
Total
Magnification
FOV in mm
FOV in μm
scanning
low power
high power
oil
immersion
Exercise 2B - Estimation of Size
Using FOV from your microscope, determine the approximate size of a single Euglena and Paramecium.
Add your measure bar!
Euglena (high power)
Paramecium (low power)
Estimated # across ______
total magnification ______
FOV _____
length _______
Estimated # across ______
total magnification______
FOV ______
length _______
Exercise 4B – Osmotic Conditions
Describe the appearance of your wet mount specimen and what happens with the addition of the NaCl
solution.