Mr. Rocco z Penn Trafford High School Forensic Entomotoxicology z The Maggot Mystery z Entomotoxicology Entomotoxicology is used to study the effect of toxins in the cadaver with the help of the insects. z How is it used? Arthropods are used to identify whether the toxins were present in a body at the time of death or not study the effects of toxins on the developmental frequency of insects that feeds on the cadaver study the effects of these toxins on the cadaver when the other cadaver tissues are absent The organic materials of entomotoxicological use can be investigated are: lar-vae, pupae, adult insects, puparial cases, exuviae (cast beetle skins), beetle faecal material (frass), fly predators z How is it used? The sampling sites for drug exposure in insects are the core organs (e.g. liver), the head-area or muscles in cases where no core organs are left. Later the accomplishment of sample collection from the body, they are washed by using normal water and the specimens are freeze for storing at an extreme low temperature lies between 68°F to 39.2°F. Insect samples are mainly of two types: Organic and In-organic. Drugs are distributed in the body according to their physicochemical properties z How to Sample So…different drug concentrations in different organs and tissues Thus also in insects reared on these different substrates. The best sampling sites for drug detection in insects are the internal organs (e.g. liver), the head-area or muscles in cases where no internal organs are left. z What drugs have been detected Substances like Cocaine, Heroin, Morphine, methamphetamine, Methylene Dioxymethamphetamine, Triazolam, Oxazepam, Chloripriamine, Barbiturates, Malathion, Nortriptyline and Amitryptiline, and Paracetamol z Cocaine and Meth Cocaine and methamphetamine accelerate the rate of fly development. Cocaine causes larvae to “develop more rapidly 36 to 76 hours after hatching”. The amount of methamphetamine, on the other hand, affects the rate of pupal development. A lethal dose of methamphetamine increases larval development through approximately the first two days and afterwards the rate drops if exposure remains at the median lethal dosage Methamphetamine found in larvae will untimatley seed up development but lead to increased mortality z Heroin of the effects of heroin on fly development has shown that it actually speeds up larval growth and then decreases the development rate of the pupal stage. 1. Select one sample z from each of the Maggot maggot collections (choose, A, B, C and D) 2. Add a small amount of alcohol to each sample. Crush the maggot sample (6-10 maggots) in a vial or small dish 3. Allow the alcohol to evaporate 4. Take a few drops of the Maggot A Soup and place it in 4 separate spot plate wells Our Lab Stew: Drug Detection z Our Lab Maggot Stew: Drug Detection 5. Place of few drops of the 4 drug indicators into each of the wells. A positive result will result in color change and indicate a positive test for the presence of a specific drug. Each indicator is specific for a drug.