(ii) To 2mL of the alcoholic solution, a few
drops of saturated solution of NaHCO„, are
added.
(iii) 100mg of the sample is shaken with
2mL of K103/KI mixture and 2m14 of ClIC1„ or
CC14 in a test tube.
(iv) 500mg of the sample is warmed with
2mL of CH 3 OH or C,H 2 OH and 2 drops of
conc. H 2 SO 4 . The product. is poured into 20mL
of water contained in a small beaker.
265
Effervescence of CO2
-000/1 group
present
Violet
colour
in
organic solvent layer
-COOH group
present
Characteristic sweet
smell of an ester
-COOff group
present
2. Test for alcoholic -OH group:
(i) 200mg of the sample is dissolved in 5mL of
anhydrous benzene and a thin piece of metallic
Na is added.
Effervescence of H2
gas
ii) 100mg of the sample is added to lmL of
ceric ammonium nitrate solution.
Pink or red colour
Alcoholic -OH group
present
Alcoholic -OH group
present
3. Test for phenolic -OH group:
(i) To 2mL of the alcoholic solution of the
sample, 2 drops of neutral FeC1 3 solution are
added..
Red, blue, green or
violet colour
Phenolic -OH group
present
•
(ii) Back Dye Test:
A few drops of aniline are dissolved in 5mL of Rose red dye
dilute HC1, cooled and diazotised by adding a
pinch of NaNO2. This diazotised solution is then
poured into the cold alkaline solution of the
sample.
Phenolic -OH group
present
4. Test for Ester group:
(i) A small quantity of the sample is hydrolysed
by a ser,Qng solution of NaOH. The solution is
cooled and then acidified with dilute 11C1.
(ii)
10
Ester group (-COOR)
present
A wine red
Ester group (-COOK)
colouration
present
Feigl Test (Hydroxamic Acid Test):
Orng of the substance is taken in a test tube
a n d l m L o f 5 % s o l u t i o n o f h yd r o x yl a m i n e
-
White precipitate
-------t_
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ORGANIC QUALITATIVE ANALYSIS