Uploaded by vaaninc13

1.1-Microscopes-CIE-IAL-Biology-QP

advertisement
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
Microscopes
Question Paper 1
Level
International A Level
Subject
Biology
Exam Board
CIE
Topic
Microscopes
Sub Topic
Booklet
Multiple Choice
Paper Type
Question Paper 1
Time Allowed :
60 minutes
Score
:
/ 50
Percentage
:
/100
Grade Boundaries:
A*
>85%
A
777.5%
B
70%
C
62.5%
D
57.5%
E
U
45%
<45%
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
1
2
What are the appropriate units for measuring diameters of alveoli, diameters of white blood cells
and the width of cell walls?
diameters of
alveoli
diameters of
white blood cells
width of
cell walls
A
mm
µm
µm
B
µm
mm
µm
C
µm
µm
nm
D
mm
m
mm
nm
The diagram shows a stage micrometer scale on which the small divisions are 0.1 mm. It is
viewed through an eyepiece containing a graticule.
0
10
20
30
40
50
60
70
80
90 100
The stage micrometer scale is replaced by a slide of a plant cell.
0
10
20
30
40
50
60
70
80
90 100
What is the width of a chloroplast?
A
0.5 mm
B
10 µm
C
50 µm
D
100 µm
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
3
The electronmicrograph shows a cell.
×5700
What is the actual diameter of the nucleus?
0.6 µm
A
4
5
B
6 µm
C
35 µm
D
350 µm
What is the diameter of a typical plant cell?
A
4.0 × 101 µm
B
1.0 × 100 µm
C
4.0 × 102 nm
D
1.0 × 102 nm
A specimen is viewed under a microscope using green light with a wavelength of 510 nm.
If the same specimen is viewed under the same conditions, but using red light with a wavelength
of 650 nm instead, what effect will this have on the magnification and on the resolution of the
microscope?
magnification
resolution
A
decreased
remains the same
B
increased
ncreased
C
remains the same
decreased
D
remains the same
increased
increased
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
6
7
8
9
Which statements about light microscopes are correct?
1
To calculate the magnification of a light microscope the eyepiece lens and objective
lens magnifications are added together.
2
As the magnification increases the resolution decreases.
3
The resolution of a light microscope is limited by the wavelength of light.
4
The scale on a stage micrometer is resolved more clearly than an eyepiece
graticule.
A
1,
1 2 and 3
B
1,
1, 3 and 4
C
2 and 3 only
D
2 and 4 only
What is the diameter of a typical prokaryote, such as Streptococcus?
A
7.5 × 101 nm
B
7.5 × 102 nm
C
7.5 × 100 µm
D
7.5 × 101 µm
What best describes an electron microscope in comparison with a light microscope?
magnification
resolution
A
higher
higher
B
higher
lower
C
lower
higher
D
lower
lower
What is the order of size of cell structures?
largest
smallest
A
e
centrioles
ribosomes
lysosomes
nucleoli
B
lysosomes
nucleoli
centrioles
ribosomes
C
nucleoli
lysosomes
centrioles
ribosomes
D
nucleoli
centrioles
ribosomes
lysosomes
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
10 At approximately which magnification is light microscopy not suitable because the resolution
becomes too low?
A
×100
×200
B
C
×400
D
×1500
11 The diagram shows a stage micrometer viewed with an eyepiece graticule scale, using a
magnification of ×400.
0.1mm
0
10
20
30
40
50
60
70
80
90 100
Using the same magnification, a chloroplast is measured and found to be 4 eyepiece graticule
divisions long.
How long is the chloroplast?
A
1.0 × 101 µm
B
4.0 × 102 µm
C
2.5 × 10–1 µm
D
2.5 × 10–2 µm
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
12 The diagram shows an electron micrograph of a plant cell.
X
Y
Z
What do structures X, Y and Z contain?
X
Y
Z
A
air
starch
DNA and RNA
B
cell sap
chlorophyll
protein
C
mineral ions
starch
DNA and RNA
D
water
mineral ions
starch
13 The eyepiece lens of a microscope is fitted with an eyepiece graticule.
Which statements about the graticule are correct?
A
1
It allows you to measure the actual length of cells.
2
It allows you to draw cells with correct proportions.
3
It changes in size as the objective lens changes from ×10 to ×40.
1, 2 and 3
B
1 and 3 only
C
1 only
D
2 only
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
14 The diameter of living cells varies considerably.
The diameter of a typical eukaryotic cell is 1.5 × 101 µm.
The diameter of a typical prokaryotic cell is 7.5 × 102 nm.
Using these measurements, what is the maximum number of each cell type which could fit along
a line 1 cm long?
number of white
blood cells
number of
Streptococcus cells
A
6.7 × 104
1.3 × 102
B
6.7 × 103
1.3 × 105
C
6.7 × 102
1.3 × 104
D
6.7 × 101
1.3 × 103
15 Which size ranges can be viewed using a light microscope?
1
1 nm
A
4 only
B
1 and 2 only
C
2 and 3 only
D
3 and 4 only
2
3
1 µm
100 nm
4
100 µm
1 mm
16 The diagram shows a mitochondrion drawn from an electronmicrograph.
Y
X
The length of the mitochondrion from X to Y is 3000 nm.
What is the magnification of the drawing of the mitochondrion?
A
×100
B
×1000
C
×10 000
D
×100 000
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
17 The diagram shows a stage micrometer, with divisions 0.1 mm apart, viewed through an eyepiece
containing a graticule.
0 10 20 30 40 50 60 70 80 90 10
What is the area of the field of view of the microscope at this magnification? (π = 3.14)
× 102 µm2
A
π × 12.5 × 12.5
1
B
π × 50 × 50
5 =
C
π × 125 × 1
125
× 104 µm2
D
π × 250 × 250
2
× 105 µm2
× 103 µm2
18 A light microscope is used to observe two membranes that are 200 nm apart.
How far apart are the membranes when the objective lens is changed from low power (×40) to
high power (×400)?
A
2 µm
B
20 µm
C
200 nm
D
2000 nm
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
19 The electronmicrograph is of a chloroplast.
The length of the chloroplast along the line shown is 80 mm. The actual length of the chloroplast
is 10 µm.
What is the magnification of the chloroplast?
A
×8 × 102
B
×8 × 103
C
×8 × 104
D
×8 × 106
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
20 The diagram below is drawn from an electronmicrograph of an animal cell.
Which represents the same cell, seen under a light microscope at ×400 magnification?
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
21 An electron microscope has a higher resolution than a light microscope.
Which is a result of the higher resolution?
A
the ability to produce larger images of cells
B
the ability to see cristae in mitochondria
C
the ability to see mRNA in all cells
D
the ability to see the nucleus in eukaryotes
22 The diagram shows a chloroplast drawn from an electronmicrograph.
X
Y
The length of the chloroplast from X to Y is 5000 nm.
What is the magnification of the drawing of the chloroplast?
A
×100
B
×1000
C
×10 000
D
×100 000
23 Eyepiece graticules and stage micrometers are used to measure cells.
Which is the correct reason why an eyepiece graticule is calibrated?
A
An eyepiece graticule can be used to make measurements.
B
An eyepiece graticule is magnified by the objective lens.
C
An eyepiece graticule magnifies the specimen.
D
An eyepiece graticule makes comparisons.
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
24 Which group of structures are visible in a suitably stained plant cell using a high power (x400)
light microscope?
centriole
cilia
mitochondria
starch grains
A
key
B
= visible
C
= not visible
D
25 The magnification of the photomicrograph is ×4000.
What is the actual size of the nucleolus?
A
1 µm
B
2 µm
C
5 µm
D
20 µm
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
26 A microscope has a resolution of 200 nm. Which of the following organelles would not be
resolved using this microscope?
A
chloroplasts
B
lysosomes
C
mitochondria
D
ribosomes
27 Which range of sizes would include most eukaryotic cells?
µm
A
1 × 102 nm
B
mt
1 µm
C
mt
1 × 101 µm
× 102 µm
D
1 × 102 µm
mt
× 103 µm
× 101 µm
28 What restricts the resolution of the light microscope?
A
the inability to cut very thin sections
B
the low light intensity of microscope lamps
C
the low magnification produced by glass
D
the wavelengths of visible light
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
29 The diagram shows an electron micrograph of virus particles in a human nucleus.
virus particles
×24 000
What is the diameter of the labelled virus particles?
A
1.5 × 100 µm
B
1.5 × 10–2 µm
C
1.5 × 100 nm
D
1.5 × 102 nm
D
4 × 104 µm
30 A cell organelle measures 4 × 10–1 mm in diameter.
What is the diameter in µm?
A
4 × 101 µm
B
4 × 102 µm
C
4 × 103 µm
31 In the following table, which is the correct comparison between light and electron microscopes?
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
32 Plant cells are stained and then viewed through a light microscope.
Which structures would be clearly visible at a magnification of ×400?
A
chloroplast grana
B
lysosomes
C
nucleoli
D
ribosomes
33 Using a stage micrometer scale, one unit of an eyepiece graticule was calculated as 0.005 mm.
The diameter of a spongy mesophyll cell was counted as 3.5 units on the eyepiece graticule.
What is the estimate of the diameter of the cell?
A
0.18 µm
B
1.8 µm
C
18.0 µm
34 Which structures are measured using these units?
D
180 µm
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
35 The diagram below is drawn from an electron micrograph of an animal cell.
Which represents the same cell, seen under a light (optical) microscope at ×400 magnification?
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
36 The diagram shows a stage micrometer, with divisions 0.1 mm apart, viewed through an eyepiece
containing a graticule.
0 10 20 30 40 50 60 70 80 90 10
What is the area of the field of view of the microscope at this magnification? (π = 3.14)
× 102 µm2
A
π × 12.5 × 12.5
1
B
π × 55 × 55
5 =
C
π × 125 × 1
125
× 104 µm2
D
π × 250 × 250
2
× 105 µm2
× 103 µm2
37 When making measurements in experiments, which methods have parallax errors?
1
using a calibrated eyepiece graticule to measure length
2
using a measuring cylinder to measure volume
3
using a ruler to measure length of a shoot
A
1 and 2 only
B
2 and 3 only
C
3 and 1 only
D
1, 2 and 3
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
38 What describes the features of an electron microscope?
maximum
magnification
m
resolution / nm
A
2.5 × 103
2.5 × 102
dead
B
2.5 × 104
5.0 × 10–1
living
C
2.5 × 105
5.0 × 10–1
dead
D
5.0 × 105
2.5 × 102
living
specim
39 The magnification of this electron micrograph is 5 × 103.
What is the actual size of the nucleolus?
A
0.2 µm
B
0.5 µm
C
2 µm
D
20 µm
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
40
The diameter of living cells varies considerably.
Typical diameters are:
a prokaryote, such as Streptococcus
-
750 nm
a eukaryotic cell, such as a white blood cell -
15 µm
Given these measurements, the diameter of the white blood cell is how many times greater than
the prokaryote?
A
B
x2
C
x 20
D
x 50
x 200
41 The diagram shows a graduated slide, with divisions of 0.1 mm viewed using an eyepiece
graticule.
0
10 20 30 40 50 60 70 80 90 100
Pollen grains were grown in a sugar solution and viewed using the eyepiece graticule. Diagram 1
shows the pollen grains at first and diagram 2 shows them after four hours.
diagram 1
0
diagram 2
0
10 20 30 40 50 60 70 80 90 100
at start
10 20 30 40 50 60 70 80 90 100
after 4 hours
What is the growth rate of the pollen tubes?
A
5 µmh–1
B
10 µmh–1
C
5 mmh–1
D
10 mmh–1
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
42 What is the resolution, in nanometres, of an electron microscope and of a light microscope?
43 The diagram shows a cell surface membrane. The lipid bilayer has an approximate width of
8 nm.
How many times has the diagram been magnified?
A
2.5 × 102
B
2.5 × 104
C
2.5 × 106
D
2.5 × 108
400 nm
D
4000 nm
44 A lysosome measures 0.4 µm in diameter.
What is the diameter in nm?
A
4 nm
B
40 nm
C
45 What describes resolution in microscopy?
A
the ability to distinguish between two objects that are very close together
B
the clarity of the image formed by the microscope
C
the number of times the image has been magnified by the objective lens
D
the power of the microscope to focus on very small objects
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
46 What is responsible for the high resolution of the electron microscope?
A
high magnification
B
short wavelength of the electron beam
C
use of heavy metal stains
D
very thin sections
47 When making measurements in experiments, which methods could have parallax errors?
A
1
using a calibrated eyepiece graticule to measure length
2
using a measuring cylinder to measure volume
3
using a ruler to measure length of a shoot
1 and 2 only
B
1 and 3 only
C
2 and 3 only
D
1, 2 and 3
48 Which combination of lenses for a light microscope will give the greatest magnification?
Save My Exams! – The Home of Revision
For more awesome GCSE and A level resources, visit us at www.savemyexams.co.uk/
49 Which steps are needed to find the actual width of a xylem vessel viewed in transverse section
using a ×10 objective lens?
A
1
Convert from mm to µm by multiplying by 10–3.
2
Calibrate the eyepiece graticule using a stage micrometer on ×4 objective lens.
3
Measure the width of the xylem vessel using an eyepiece graticule.
4
Multiply the number of eyepiece graticule units by the calibration of the eyepiece
graticule.
1 , 2 and 3
B
2,
2, 3 and 4
C
1 and 2 only
D
3 and 4 only
50 The eyepiece of a microscope is fitted with an eyepiece graticule and a stage micrometer scale is
placed on the microscope.
Which statements about the stage micrometer scale are correct?
A
1
It allows you to measure the actual length of cells.
2
It allows you to calibrate the eyepiece graticule.
3
It changes in size as the objective lens changes from ×10 to ×40.
1, 2 and 3
B
2 and 3 only
C
1 only
D
2 only
Download