Search Upgrade to Teacher Create kadaaga_na… Forensic Serology Leave the first rating STUDY Flashcards Learn Write Spell What are blood antigens? Test PLAY Match Gravity Live 1/36 Created by wbowers17 Terms in this set (36) Original What are blood antigens? 3 antigens with shared characteristics. A, B, and D. What antigens do individuals A-A have with type A, B, AB or O B-B blood type? AB - A and B O - None What antigens do individuals D antigens. have with Rh + or Rh - blood? What are antibodies? Antibodies - produced by body and bind with antigens. What is agglutination? How Agglutination occurs when someone has an does it happen? incompatible kind of blood injected into their body. As a result, the blood clots together. How can agglutination Antigens and antibodies can be found on the blood reactions be used to determine cells in agglutination. blood type? What is blood serum? Serum - yellow fluid that remains after clotting of blood Erythrocytes? Erythrocytes - "red" cells that hold hemoglobin and transport oxygen Leukocytes? Leukocytes - immune "white" blood cells Platelets? Platelets - cell fragments that cause clotting What are immunoassay Techniques that are used to identify antibodies. techniques? What is enzyme-multiplied Enzyme-multiplied immunoassay technique - adds immunoassay technique? How antibodies to urine that bind to specific drugs. is it done? What is it used for? It is used for detecting drugs in serum and urine. Upgrade to remove ads What is radioimmunoassay? Only $3/month Radioimmunoassay (RIA)— a specific test that uses radioactive tags to find drugs. It is used measuring the amounts of antibodies such as hormones or drugs. What are polyclonal and Polyclonal antibodies are made for different types monoclonal antibodies? that all do the same thing. Monoclonal are manufacturing antibodies in an identical way. What is a presumptive test? Presumptive tests are designed to do one of the How is it different from a following: definitive test? Eliminate the identity of a particular substance Establish the probability (presumptively positive) of a substance identity Definitive tests are used to specify the identity of a substance. What color tests can be used Benzidine color tests and Kastle-Meyer tests can be as presumptive blood test? used. What is the Kastle-Meyer test? It is a presumptive blood test that is used to detect How is it done? What is it used the presence of hemoglobin. for? What is the luminol test? Presumptive blood test Reaction with blood emits light Spray on suspected area, turn out lights, see blue light What are microcrystalline tests? Oldest method of blood detection What are the two most Takayama test common? Teichmann tests What is the precipitin test? It is a confirmatory test to determine if a blood stain What is it used for? came from a human or an animal. What can be determined from Using the different properties of the blood spatter, blood spatter analysis? the origin of the spatter can be determined. Why are semen stains Semen stains are analyzed because they can be analyzed? used to extract cells for DNA typing. Upgrade to remove ads Only $3/month What is the acid phosphatase Acid phosphatase is an enzyme made by the test? prostate gland. It is used to detect the presence of semen. How can using a microscope When the semen stain is dissolved in water, the be used to identify semen? presence of sperm cells can be determined. What evidence is collected All samples of DNA and all evidence (Ex. clothing) following a rape or sexually- that the victim had are collected. related crime? What evidence is collected All samples of DNA and all clothing is collected from suspects? from suspects. How should DNA evidence be Package each DNA stained item separately in paper stored? bag or well-ventilated container; closed containers can lead to moisture and growth of DNA digesting bacteria. What is the structure of DNA? Double helix shape. How is DNA different from one The sequence of nucleotides are different. individual to the next? What are restriction enzymes? AN enzyme that cuts DNA at the restriction sites. It is a defense mechanism against viruses. What is PCR? How is it done? PCR takes a sample of DNA and amplifies it but What is it used for? producing copies of a chunk of DNA. It is best used for repetitive strands of DNA. What are RFLPs? What are RFLP - restriction fragment length polymorphism; VNTRs? What are STRs? How is process requires large amounts of DNA each one used in forensic VNTR - variable number tandem repeats; repetitive analysis? DNA, vary in length STR - short tandem repeats; easy to amplify with PCR They are all used in DNA fingerprinting. Why is repetitive DNA a better Because there's much more variation in length of choice for DNA typing than non-coding DNA. coding gene DNA? Why are STRs (Short Tandem Because STRs are easy to reproduce with PCR. Repeats) used more often in forensic analysis than VNTRs (Variable Number Tandem Repeats)? How can gender be The DNA fingerprinting will appear different. determined with STR analysis and fingerprinting? What is mDNA? A type of DNA found outside nucleus in mitochondria. It is also an easier type of DNA to get. 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